FastQCFastQC Report
Mon 6 Jun 2016
ERR1211378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809160
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313370.35573198806696665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229840.2609102343469752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166660.18918943463394922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG91450.051.7209852
GGTATCA158550.044.5117571
ACCTGGG109550.042.1371573
GTATCAA278650.040.7800141
ATAACGC11100.033.455713
GTACATG341700.032.5108221
CCTGGGG106200.032.2235224
TATCACG7900.032.1700062
GTACCTG149950.032.0466651
ATCAACG350450.031.950813
CAACGCA351950.031.934845
TATCAAC357250.031.551332
TACATGG345000.031.539382
TAACGCA11750.031.2049064
TCAACGC363400.030.9286384
AACGCAG372550.030.6236046
ACATGGG348050.029.902133
ATGGGGG220800.029.7415435
CATGGGG273850.029.5244544
TGGGGGG248750.028.7433386
Produced by FastQC (version 0.11.5)