FastQCFastQC Report
Mon 6 Jun 2016
ERR1211378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809160
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278890.31659091218686003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214980.24404142960282252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163290.18536387124311512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG95250.050.2749332
GGTATCA170100.043.311361
ACCTGGG111050.042.403463
GTATCAA281600.037.4985281
CCTGGGG102900.034.2535364
TAACGCA11050.033.5988434
GTACCTG156200.031.0022561
CTGGGGG71550.030.865015
GTACATG327600.029.7647291
TGGGGGG212150.029.5669486
ATCAACG354250.029.2782173
CAACGCA357700.029.0434365
TATCAAC361900.028.9703182
TACATGG331900.028.7287712
ATAACGC12950.028.6687973
TCAACGC368250.028.254944
ATGGGGG204000.027.8237045
AACGCAG380200.027.7071466
ACATGGG333100.027.5254733
CATGGGG259100.027.007654
Produced by FastQC (version 0.11.5)