##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211378_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8809160 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98399518228753 31.0 31.0 34.0 30.0 34.0 2 32.12407596183972 33.0 31.0 34.0 30.0 34.0 3 32.19469086723365 34.0 31.0 34.0 30.0 34.0 4 35.864836147827944 37.0 35.0 37.0 35.0 37.0 5 35.723427432354505 37.0 35.0 37.0 35.0 37.0 6 35.75686285639039 37.0 35.0 37.0 33.0 37.0 7 35.68698752207929 37.0 35.0 37.0 33.0 37.0 8 35.665751445086705 37.0 35.0 37.0 33.0 37.0 9 37.23132489363344 39.0 37.0 39.0 33.0 39.0 10-11 37.198280085728946 39.0 37.0 39.0 33.0 39.0 12-13 37.10501722071117 39.0 37.0 39.0 33.0 39.0 14-15 38.27285223562746 40.0 38.0 41.0 33.0 41.0 16-17 38.25674667051115 40.0 38.0 41.0 33.0 41.0 18-19 38.26644129519727 40.0 38.0 41.0 33.5 41.0 20-21 38.229386456824486 40.0 38.0 41.0 33.5 41.0 22-23 38.125448964486964 40.0 37.0 41.0 33.0 41.0 24-25 38.004305972419616 40.0 37.0 41.0 33.0 41.0 26-27 37.873837006025546 40.0 37.0 41.0 33.0 41.0 28-29 37.78291522687748 40.0 37.0 41.0 33.0 41.0 30-31 37.81712722892989 40.0 37.0 41.0 33.0 41.0 32-33 37.64104165436886 40.0 36.5 41.0 33.0 41.0 34-35 37.530975938682005 40.0 36.0 41.0 32.0 41.0 36-37 37.39057503780156 40.0 36.0 41.0 32.0 41.0 38-39 37.27059855877292 39.5 36.0 41.0 31.0 41.0 40-41 37.20297497150693 39.0 36.0 41.0 31.0 41.0 42-43 37.07122069527628 39.0 36.0 41.0 31.0 41.0 44-45 36.92443859573444 39.0 35.5 41.0 31.0 41.0 46-47 36.70595715141966 39.0 35.0 41.0 30.0 41.0 48-49 36.49073407680187 39.0 35.0 41.0 30.0 41.0 50-51 36.30973963465302 39.0 35.0 40.0 29.5 41.0 52-53 36.104384810810565 39.0 35.0 40.0 29.0 41.0 54-55 35.9059301340877 38.0 35.0 40.0 28.0 41.0 56-57 35.67816369551694 38.0 34.5 40.0 28.0 41.0 58-59 35.41960306090479 38.0 34.0 40.0 27.5 41.0 60-61 35.15489252096681 37.5 34.0 40.0 27.0 41.0 62-63 34.846742311412214 37.0 34.0 40.0 26.0 41.0 64-65 34.49203272502713 36.5 33.0 39.0 26.0 41.0 66-67 34.1429340595471 36.0 33.0 39.0 26.0 40.5 68-69 33.78263688024738 35.5 33.0 39.0 25.5 40.0 70-71 33.40580327749751 35.0 32.0 38.0 25.0 40.0 72-73 33.04418639234615 35.0 32.0 37.5 24.5 39.5 74-75 32.66814877922526 35.0 31.5 37.0 24.0 39.0 76-77 31.682893147587286 34.0 30.5 36.0 23.0 38.0 78-79 32.183281890668354 34.5 31.0 36.0 24.0 38.0 80-81 32.09131409805248 34.5 31.0 35.5 24.0 37.0 82-83 31.81369279250235 34.0 31.0 35.0 24.0 37.0 84-85 31.5301024161214 34.0 31.0 35.0 23.0 36.5 86-87 31.273982422841677 34.0 31.0 35.0 23.0 36.0 88-89 31.05475448283378 34.0 30.5 35.0 21.5 36.0 90-91 30.830911800898157 34.0 30.0 35.0 20.0 35.0 92-93 30.638437263030752 34.0 30.0 35.0 20.0 35.0 94-95 30.455561994560206 34.0 30.0 35.0 20.0 35.0 96-97 30.26980200155293 34.0 29.0 35.0 19.0 35.0 98-99 30.07279786040894 34.0 29.0 35.0 18.5 35.0 100 28.289937746618293 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23614157790964896 1101 2 -0.224255340326728 1101 3 -0.29621235186948525 1101 4 -0.14351339921253015 1101 5 -0.15262678543804498 1101 6 -0.20152670832120378 1101 7 -0.2565137175890797 1101 8 -0.30911723529775514 1101 9 -0.4503605595751097 1101 10-11 -0.3945979920473732 1101 12-13 -0.4206790118457491 1101 14-15 -0.5923446429381016 1101 16-17 -0.6324796383808575 1101 18-19 -0.6503832638258729 1101 20-21 -0.6416741446771468 1101 22-23 -0.7625795176516661 1101 24-25 -0.7424947023156321 1101 26-27 -0.7618875589522034 1101 28-29 -0.81642763176297 1101 30-31 -0.7109232538908614 1101 32-33 -0.6058302463029861 1101 34-35 -0.7556313698182819 1101 36-37 -0.7310078404520155 1101 38-39 -0.7288591892884781 1101 40-41 -0.8146539694953745 1101 42-43 -0.7503645642448546 1101 44-45 -0.7816606414538043 1101 46-47 -0.7973608675258888 1101 48-49 -0.7233695460713463 1101 50-51 -0.8222877997391294 1101 52-53 -0.8023709075612757 1101 54-55 -0.8602621941938864 1101 56-57 -0.867760772085461 1101 58-59 -0.845371469388752 1101 60-61 -0.8833752489966571 1101 62-63 -0.8622119297876196 1101 64-65 -0.7670451828475251 1101 66-67 -0.8743282046096255 1101 68-69 -0.8435539986959171 1101 70-71 -1.0328537724127287 1101 72-73 -0.9679550049808796 1101 74-75 -1.0118530459940231 1101 76-77 -0.8641909324287909 1101 78-79 -0.7350330665396037 1101 80-81 -0.6560835315711948 1101 82-83 -0.7612910666294574 1101 84-85 -0.7962713688548 1101 86-87 -0.8705255776404144 1101 88-89 -0.8673544903600146 1101 90-91 -1.0591213890203477 1101 92-93 -1.151885236305862 1101 94-95 -1.1436741406210054 1101 96-97 -1.0842631396226494 1101 98-99 -1.165373278365827 1101 100 -1.2481117414511829 1102 1 -0.3124793429160313 1102 2 -0.2837904062951111 1102 3 -0.3872437210593809 1102 4 -0.1836429326390956 1102 5 -0.250700042683242 1102 6 -0.325423568694589 1102 7 -0.29811473240694397 1102 8 -0.36683102050663763 1102 9 -0.46280108127256625 1102 10-11 -0.48099145741353055 1102 12-13 -0.490584023846651 1102 14-15 -0.6215271838624048 1102 16-17 -0.7641051082102095 1102 18-19 -0.696786549812515 1102 20-21 -0.727636549690267 1102 22-23 -0.6826567684503999 1102 24-25 -0.814624173337883 1102 26-27 -0.8358622361468946 1102 28-29 -0.911288662585477 1102 30-31 -0.7590607817737549 1102 32-33 -0.7471274978250406 1102 34-35 -0.7560173192041262 1102 36-37 -0.7759033499783712 1102 38-39 -0.8477681208344947 1102 40-41 -0.9052881719956858 1102 42-43 -0.877907757472137 1102 44-45 -0.7907981104136255 1102 46-47 -0.8944209492097812 1102 48-49 -0.9526798175952109 1102 50-51 -0.9564561082258081 1102 52-53 -0.9663576942866214 1102 54-55 -1.0280502205473354 1102 56-57 -0.9959460995427349 1102 58-59 -1.1037356212970906 1102 60-61 -1.0966805635682135 1102 62-63 -1.0787350713753199 1102 64-65 -1.0754044545840742 1102 66-67 -1.0756913616591675 1102 68-69 -1.2072317986654753 1102 70-71 -1.1584203187770328 1102 72-73 -1.279755128229393 1102 74-75 -1.2794705548255543 1102 76-77 -1.032670297650597 1102 78-79 -0.989005313640817 1102 80-81 -0.8614342708720955 1102 82-83 -0.8945342296022467 1102 84-85 -1.0290558456407517 1102 86-87 -1.0578841243781163 1102 88-89 -1.0250723273363604 1102 90-91 -0.9678311595948941 1102 92-93 -1.103245580971059 1102 94-95 -1.1264969190194982 1102 96-97 -1.1727645071989343 1102 98-99 -1.214236054897345 1102 100 -1.154927584719406 1103 1 -0.3734975858589884 1103 2 -0.3581873324252278 1103 3 -0.3871976870552736 1103 4 -0.24483792342498845 1103 5 -0.19356192232226732 1103 6 -0.3697199880609574 1103 7 -0.34980601522281063 1103 8 -0.46274799107621334 1103 9 -0.5900935949743769 1103 10-11 -0.5523316201254644 1103 12-13 -0.5931275527728133 1103 14-15 -0.7686660609022482 1103 16-17 -0.8267425140292701 1103 18-19 -0.7449225623519524 1103 20-21 -0.7317378709392628 1103 22-23 -0.9046897632213557 1103 24-25 -0.782970380225251 1103 26-27 -0.8801779297153374 1103 28-29 -0.8272091372601977 1103 30-31 -0.8162040089052098 1103 32-33 -0.7853004366180016 1103 34-35 -0.7965859747400401 1103 36-37 -0.7462306996489332 1103 38-39 -0.7890692447624161 1103 40-41 -0.9129618781032391 1103 42-43 -0.9280009052903253 1103 44-45 -0.9932300210878466 1103 46-47 -1.0281123437413342 1103 48-49 -1.0557435470055552 1103 50-51 -1.0840100333675338 1103 52-53 -1.192381894045944 1103 54-55 -1.1557051119809572 1103 56-57 -1.1763909763802616 1103 58-59 -1.3196406538773076 1103 60-61 -1.2097988907688233 1103 62-63 -1.2238626034282234 1103 64-65 -1.1201806830465273 1103 66-67 -1.2746013219075465 1103 68-69 -1.2800580640606825 1103 70-71 -1.2259004937094957 1103 72-73 -1.3453177636217326 1103 74-75 -1.3315367103172164 1103 76-77 -1.179075293614705 1103 78-79 -0.9910149699023947 1103 80-81 -0.9567540659322802 1103 82-83 -0.95609994432035 1103 84-85 -0.9523064438798343 1103 86-87 -1.098845725198835 1103 88-89 -1.0366638600673497 1103 90-91 -1.1829456657295978 1103 92-93 -1.1133458474161984 1103 94-95 -1.1508209701547507 1103 96-97 -1.1971193532155944 1103 98-99 -1.2104802728396962 1103 100 -1.364015168025123 1104 1 -0.43262238264482633 1104 2 -0.3551111993011027 1104 3 -0.3841567403331183 1104 4 -0.21584174120690847 1104 5 -0.3063492895361719 1104 6 -0.32356680817788686 1104 7 -0.33189289468998595 1104 8 -0.33290748257130076 1104 9 -0.5014444269796954 1104 10-11 -0.43487825602544916 1104 12-13 -0.5306823810954313 1104 14-15 -0.6950957233829484 1104 16-17 -0.7214084094003965 1104 18-19 -0.7979450480837755 1104 20-21 -0.6761825127739414 1104 22-23 -0.8102570739569117 1104 24-25 -0.8159378284644845 1104 26-27 -0.8835461967547431 1104 28-29 -0.9002460544925839 1104 30-31 -0.8492444906232635 1104 32-33 -0.7151345758765828 1104 34-35 -0.7885834669925629 1104 36-37 -0.8027752394283141 1104 38-39 -0.9057838008480559 1104 40-41 -0.9942962777804851 1104 42-43 -0.9424450788462266 1104 44-45 -0.9884802989032337 1104 46-47 -0.9241472914193665 1104 48-49 -0.9538589950803882 1104 50-51 -0.9285679872053976 1104 52-53 -0.8743779503527023 1104 54-55 -0.8962955329292015 1104 56-57 -0.8845997642046157 1104 58-59 -0.9709865378460165 1104 60-61 -1.049947819793971 1104 62-63 -1.0308423804980578 1104 64-65 -0.9441466670140386 1104 66-67 -0.9776595916698483 1104 68-69 -0.9185162774657982 1104 70-71 -0.9901772002950153 1104 72-73 -0.9052641276907991 1104 74-75 -0.8430955252313375 1104 76-77 -0.9006194494382314 1104 78-79 -0.73908944358406 1104 80-81 -0.8280473487679139 1104 82-83 -0.8955088946341725 1104 84-85 -0.7841677554239048 1104 86-87 -0.7552000062574677 1104 88-89 -0.8345464622499534 1104 90-91 -0.8262105233884007 1104 92-93 -1.067498866185172 1104 94-95 -1.15822667624629 1104 96-97 -1.1480028364499866 1104 98-99 -1.1177657982935116 1104 100 -1.0933834353920027 1105 1 -0.42996425186231946 1105 2 -0.4099929201732415 1105 3 -0.4441167347993762 1105 4 -0.2886442132177862 1105 5 -0.33360954849255364 1105 6 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1105 68-69 -1.145530901174709 1105 70-71 -1.1176054120845507 1105 72-73 -1.2061473019604065 1105 74-75 -1.221610206446428 1105 76-77 -1.1170571970401575 1105 78-79 -1.0235531554134845 1105 80-81 -0.9246070860705586 1105 82-83 -0.9661141630108894 1105 84-85 -0.9212434087725363 1105 86-87 -0.8823010464259227 1105 88-89 -0.9175647430550455 1105 90-91 -0.8779116506483575 1105 92-93 -0.8988679516323366 1105 94-95 -0.9542634607956479 1105 96-97 -1.0522322830895732 1105 98-99 -1.0581710283093742 1105 100 -1.1366384552617532 1106 1 -0.41902901675938864 1106 2 -0.37314144793331394 1106 3 -0.4174119709612114 1106 4 -0.19556890912345182 1106 5 -0.2462817204623846 1106 6 -0.3377754989039232 1106 7 -0.3602343486191799 1106 8 -0.38996882017573853 1106 9 -0.4813133588898282 1106 10-11 -0.5094419426280723 1106 12-13 -0.5981324645591286 1106 14-15 -0.7578818066233097 1106 16-17 -0.7792406957462887 1106 18-19 -0.74358314338361 1106 20-21 -0.6863888934440823 1106 22-23 -0.703401103024774 1106 24-25 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2115 48-49 0.8596693769183403 2115 50-51 0.9165516423560405 2115 52-53 0.9572204285209764 2115 54-55 0.9306620455592736 2115 56-57 0.849501755474364 2115 58-59 0.8474194933527244 2115 60-61 0.8546560575968698 2115 62-63 0.9189032426382795 2115 64-65 0.9697615667447508 2115 66-67 0.983471885596586 2115 68-69 0.9891679242380604 2115 70-71 1.0723483864311092 2115 72-73 1.0348845447206827 2115 74-75 0.701904107732787 2115 76-77 0.6880822145852079 2115 78-79 0.5203219926019429 2115 80-81 0.7199872665706444 2115 82-83 0.7764540527335164 2115 84-85 0.9261623330113729 2115 86-87 0.8990937399213337 2115 88-89 0.8958743426600364 2115 90-91 0.9373841313405435 2115 92-93 1.0103431433936159 2115 94-95 0.9674301910325944 2115 96-97 1.0009502148656395 2115 98-99 1.1120429813425972 2115 100 0.9762355036342178 2116 1 0.17130548710218463 2116 2 0.30111380860497405 2116 3 0.3578993425599464 2116 4 0.19974972400025592 2116 5 0.32816269555926425 2116 6 0.2603991757979074 2116 7 0.35565903553412426 2116 8 0.3788090450752932 2116 9 0.5397222459027944 2116 10-11 0.5796087153602798 2116 12-13 0.45284068379443454 2116 14-15 0.6336134439110879 2116 16-17 0.7183063369086469 2116 18-19 0.6188840592388445 2116 20-21 0.7425425080646519 2116 22-23 0.8294382895845871 2116 24-25 0.8061773821540328 2116 26-27 0.82273177536117 2116 28-29 0.7935666993265897 2116 30-31 0.6057599916648329 2116 32-33 0.617861001103222 2116 34-35 0.6232490998405993 2116 36-37 0.6852695946854013 2116 38-39 0.6422251540225759 2116 40-41 0.7636038596687911 2116 42-43 0.8119915512909515 2116 44-45 0.8295846535304676 2116 46-47 0.7447925447263586 2116 48-49 0.6783525041809284 2116 50-51 0.8639191143923242 2116 52-53 0.8387109146790408 2116 54-55 0.8560454747993234 2116 56-57 0.8864391048399014 2116 58-59 0.9833032810982232 2116 60-61 0.916182509013737 2116 62-63 0.9596185684907255 2116 64-65 0.7823126988372877 2116 66-67 0.759818748823335 2116 68-69 0.7477097864728677 2116 70-71 0.6433500288661236 2116 72-73 0.822011138498631 2116 74-75 0.8251692026051458 2116 76-77 0.7764810158910649 2116 78-79 0.786937110253227 2116 80-81 0.695052489691367 2116 82-83 0.6276367495774942 2116 84-85 0.5596701810898885 2116 86-87 0.576540358855393 2116 88-89 0.5576287858043898 2116 90-91 0.4411960689646435 2116 92-93 0.5927628614053226 2116 94-95 0.728950959766987 2116 96-97 0.8304943557645501 2116 98-99 0.9259754005111382 2116 100 0.534821569330461 2201 1 0.766845081086938 2201 2 0.7011538783413158 2201 3 0.6577940083604759 2201 4 0.4362548761870073 2201 5 0.5498093968063529 2201 6 0.5538072469187796 2201 7 0.6070949283371689 2201 8 0.6305358949783582 2201 9 0.7811993628577127 2201 10-11 0.7127865890600233 2201 12-13 0.7611646157390979 2201 14-15 0.9409296728774876 2201 16-17 0.8993763031375082 2201 18-19 0.9225307285740243 2201 20-21 0.9148952404001633 2201 22-23 0.9081362255684411 2201 24-25 0.8256125961209548 2201 26-27 0.9061310465985741 2201 28-29 0.9434749745651203 2201 30-31 1.0836966552707779 2201 32-33 0.9256262496907439 2201 34-35 1.0642945057546456 2201 36-37 1.1350395285087274 2201 38-39 1.138918927261308 2201 40-41 1.1178974063405605 2201 42-43 0.230747482402073 2201 44-45 0.9039831712792861 2201 46-47 1.0987463959227668 2201 48-49 1.157772387991706 2201 50-51 1.1987646909394698 2201 52-53 1.222843821387535 2201 54-55 1.2375752261385244 2201 56-57 1.222520258989121 2201 58-59 1.227032661034734 2201 60-61 1.2225925103082034 2201 62-63 1.2720691485433946 2201 64-65 1.266834624830942 2201 66-67 1.2794836166756625 2201 68-69 1.1910182026824856 2201 70-71 1.2676670554774105 2201 72-73 1.259172052850836 2201 74-75 1.2363767503323047 2201 76-77 1.231943912413378 2201 78-79 1.0740773736918499 2201 80-81 0.9808280786202594 2201 82-83 0.9591694653960836 2201 84-85 0.9217007224403666 2201 86-87 0.9401991541453043 2201 88-89 0.9835024185518648 2201 90-91 0.3446613703216208 2201 92-93 0.7263953364419926 2201 94-95 0.9063412934481754 2201 96-97 0.9363014322667134 2201 98-99 0.9039190089768567 2201 100 1.0690129793080345 2202 1 0.7030496775583224 2202 2 0.67723515415382 2202 3 0.6414926581918223 2202 4 0.4211460297615659 2202 5 0.42303789998161534 2202 6 0.48758003089606916 2202 7 0.5517367432958693 2202 8 0.5435731108063706 2202 9 0.7461608135767008 2202 10-11 0.7272759146690149 2202 12-13 0.7480308545658545 2202 14-15 0.9358389679186843 2202 16-17 0.8963628115420974 2202 18-19 0.8734855210506467 2202 20-21 0.8670970138499072 2202 22-23 0.8753466613577814 2202 24-25 0.8391914945833534 2202 26-27 0.864795710117761 2202 28-29 0.9070431823719929 2202 30-31 1.0218524892938703 2202 32-33 0.6376195891927168 2202 34-35 1.0448015704912237 2202 36-37 1.1494077903090485 2202 38-39 1.1566913852359377 2202 40-41 1.1068443904241363 2202 42-43 1.1775098877176546 2202 44-45 1.1256885350322605 2202 46-47 1.1442321226807692 2202 48-49 1.1896760257113286 2202 50-51 1.1489252674347057 2202 52-53 1.195586745610079 2202 54-55 1.143944078803834 2202 56-57 1.1071254954039702 2202 58-59 1.126886342704175 2202 60-61 1.1144265531069095 2202 62-63 1.1924903217873322 2202 64-65 1.2514351095135652 2202 66-67 1.2408465874028565 2202 68-69 1.2587454791332249 2202 70-71 1.2870773770827242 2202 72-73 1.2258514580776065 2202 74-75 1.177830497249765 2202 76-77 1.1963161898874688 2202 78-79 1.0120218135062444 2202 80-81 1.0073270883841907 2202 82-83 1.0184412851367917 2202 84-85 0.9984018277389985 2202 86-87 1.0014826246229447 2202 88-89 0.9884772239831179 2202 90-91 1.0678120946743768 2202 92-93 1.0615956019216597 2202 94-95 1.0509928374376436 2202 96-97 0.8528760767860923 2202 98-99 0.8821833955956002 2202 100 1.1246517569571175 2203 1 0.6600774047834115 2203 2 0.6307666281815472 2203 3 0.5920902315153569 2203 4 0.3471337628020592 2203 5 0.48261857468652636 2203 6 0.5091560643479269 2203 7 0.5079077501134748 2203 8 0.4681004432039231 2203 9 0.6729786991903666 2203 10-11 0.6881604519680451 2203 12-13 0.6762096498178778 2203 14-15 0.8577705860031131 2203 16-17 0.8293594252502245 2203 18-19 0.8159162528026087 2203 20-21 0.7998935385557857 2203 22-23 0.7470109221594541 2203 24-25 0.7454406643385312 2203 26-27 0.8310402157420285 2203 28-29 0.8349706901910565 2203 30-31 0.971194455697372 2203 32-33 1.0016892777291488 2203 34-35 0.9835076394701829 2203 36-37 0.9974781998669755 2203 38-39 1.010615989412102 2203 40-41 1.0213717006818257 2203 42-43 1.1373335105075384 2203 44-45 1.1264335875111087 2203 46-47 1.1076733527862572 2203 48-49 1.0849258353085176 2203 50-51 1.10495517875718 2203 52-53 1.147756894877027 2203 54-55 1.095656301926411 2203 56-57 1.161491125629908 2203 58-59 1.168131936871653 2203 60-61 1.1517895073615279 2203 62-63 1.1696900923445668 2203 64-65 1.1940228959111323 2203 66-67 1.2095676210834938 2203 68-69 1.1923849790822985 2203 70-71 1.2155994324441863 2203 72-73 1.2282527106033996 2203 74-75 1.1938604491619813 2203 76-77 1.1369573750490432 2203 78-79 1.061186559539891 2203 80-81 0.9981681503743296 2203 82-83 0.9860466081499943 2203 84-85 0.9410929334922535 2203 86-87 0.9209226318700559 2203 88-89 0.9628813295991208 2203 90-91 1.0253090947230845 2203 92-93 1.1093243011604876 2203 94-95 1.0455913568712738 2203 96-97 1.0667403627534071 2203 98-99 1.0870386949929554 2203 100 1.2609820158016234 2204 1 0.6856263170684933 2204 2 0.6547748048085893 2204 3 0.6252485669376924 2204 4 0.4516722275830958 2204 5 0.5165254842345348 2204 6 0.5355552447493324 2204 7 0.5408019321088489 2204 8 0.564438978555323 2204 9 0.744771310608094 2204 10-11 0.7511061193842963 2204 12-13 0.765087523849445 2204 14-15 0.9289007823636339 2204 16-17 0.8868628359871948 2204 18-19 0.8944580261038126 2204 20-21 0.9178863513240358 2204 22-23 0.8919855645358368 2204 24-25 0.91100698051644 2204 26-27 0.9052333759488036 2204 28-29 0.9319594815355714 2204 30-31 1.0606584892305762 2204 32-33 1.1278663057557239 2204 34-35 1.0949929330821462 2204 36-37 1.1120676547438677 2204 38-39 1.1201703818202375 2204 40-41 1.176041224937677 2204 42-43 1.2483447798687237 2204 44-45 1.2332566866617611 2204 46-47 1.146702066431068 2204 48-49 1.2222295870430102 2204 50-51 1.194401929689441 2204 52-53 1.229110892405366 2204 54-55 1.1727626036048662 2204 56-57 1.2313249775458246 2204 58-59 1.3112648544725403 2204 60-61 1.287427422502077 2204 62-63 1.302154581379611 2204 64-65 1.339606342925741 2204 66-67 1.3391081838258856 2204 68-69 1.316027469615932 2204 70-71 1.2546560790740884 2204 72-73 1.3321135241535345 2204 74-75 1.351494439882437 2204 76-77 1.3397584798568545 2204 78-79 1.1863626904545939 2204 80-81 1.1310239551369285 2204 82-83 1.1362204752979146 2204 84-85 1.109470204216148 2204 86-87 1.1194579217312395 2204 88-89 1.163327683763498 2204 90-91 1.220176962890509 2204 92-93 1.25954982192469 2204 94-95 1.3122522547043367 2204 96-97 1.2085170218972863 2204 98-99 1.1830812303269092 2204 100 1.4248681255825595 2205 1 0.708243730842387 2205 2 0.5264795740536243 2205 3 0.6095946551234306 2205 4 0.4158246154829257 2205 5 0.5357194002996835 2205 6 0.5457628776810708 2205 7 0.5749056624247686 2205 8 0.5852507575525507 2205 9 0.7842432572461178 2205 10-11 0.7626782874126761 2205 12-13 0.7692723337070291 2205 14-15 0.9788771931826687 2205 16-17 0.9534760122772994 2205 18-19 0.9617634973419271 2205 20-21 0.94135828440605 2205 22-23 0.9458769841356514 2205 24-25 0.9349894974297683 2205 26-27 0.9807066817303323 2205 28-29 1.0155972232315236 2205 30-31 1.081522046773678 2205 32-33 1.0923526258022918 2205 34-35 1.1056198345573023 2205 36-37 1.1181928314292477 2205 38-39 1.1346806068410515 2205 40-41 1.105926146589944 2205 42-43 1.148988754592473 2205 44-45 1.1445768285392148 2205 46-47 1.1918941704204045 2205 48-49 1.2234233001146961 2205 50-51 1.2091854699501852 2205 52-53 1.2128769222363545 2205 54-55 1.1830029567649731 2205 56-57 1.1601482994039216 2205 58-59 1.2113449359918818 2205 60-61 1.234809092735091 2205 62-63 1.2447579381159244 2205 64-65 1.202633353303355 2205 66-67 1.220763050799313 2205 68-69 1.2348365145364895 2205 70-71 1.2652539140877295 2205 72-73 1.2371343023891157 2205 74-75 1.2724606081002108 2205 76-77 1.1869795547609812 2205 78-79 1.0541141465408614 2205 80-81 1.0339687054066253 2205 82-83 1.028449964552724 2205 84-85 1.0282944996418486 2205 86-87 1.0429604299579438 2205 88-89 1.0808399880520483 2205 90-91 1.11771786418689 2205 92-93 1.113793215288407 2205 94-95 1.1758229831389393 2205 96-97 1.1881346303076796 2205 98-99 1.2544673718462818 2205 100 1.3794067259782317 2206 1 0.6781461970319747 2206 2 0.6582505830334071 2206 3 0.6464130813284541 2206 4 0.3046540027229554 2206 5 0.5153485870696031 2206 6 0.5249076402019597 2206 7 0.5643061293526799 2206 8 0.5683481192980651 2206 9 0.7388147160715448 2206 10-11 0.7697743742743199 2206 12-13 0.787868377072499 2206 14-15 0.9709567447237859 2206 16-17 0.9298667858643199 2206 18-19 0.9249486613119444 2206 20-21 0.9115556531267615 2206 22-23 0.9075200949878024 2206 24-25 0.9706124847437536 2206 26-27 0.9922628858784321 2206 28-29 1.0339684689388804 2206 30-31 1.073437781873828 2206 32-33 1.0977948888800029 2206 34-35 1.083420662335918 2206 36-37 1.0759942996988556 2206 38-39 1.1280676408490322 2206 40-41 1.165365701160546 2206 42-43 1.231045875585643 2206 44-45 1.2233772090469515 2206 46-47 1.2221023597311031 2206 48-49 1.2693296827664824 2206 50-51 1.2346969386161675 2206 52-53 1.1945643944771902 2206 54-55 1.1536123016019744 2206 56-57 1.2114217114389163 2206 58-59 1.294652538953116 2206 60-61 1.254375278567366 2206 62-63 1.2943244313010638 2206 64-65 1.309065527866835 2206 66-67 1.3461423718502488 2206 68-69 1.3342712073607288 2206 70-71 1.396177495889539 2206 72-73 1.328435531269811 2206 74-75 1.3540568597805347 2206 76-77 1.28067768426002 2206 78-79 1.1692755517769697 2206 80-81 1.1031363827491383 2206 82-83 1.071128709252104 2206 84-85 1.0959379147601531 2206 86-87 1.161164797929601 2206 88-89 1.221202756466809 2206 90-91 1.2506836674260242 2206 92-93 1.2851023280617575 2206 94-95 1.3352546534404475 2206 96-97 1.3064083738245174 2206 98-99 1.3362797633575916 2206 100 1.558327828592418 2207 1 0.701971770425768 2207 2 0.668520840943863 2207 3 0.6222357178015514 2207 4 0.4151866311570984 2207 5 0.555603195260403 2207 6 0.5393418268251864 2207 7 0.5813955984871768 2207 8 0.6023865944317706 2207 9 0.8201013366586167 2207 10-11 0.7596297744317795 2207 12-13 0.8099694106928936 2207 14-15 0.9956347452807464 2207 16-17 0.9598827321536803 2207 18-19 0.9981893296302005 2207 20-21 0.9827342251986622 2207 22-23 0.9782260242997296 2207 24-25 1.0231758601507437 2207 26-27 1.024272743608428 2207 28-29 1.0279616208013493 2207 30-31 1.094460846407749 2207 32-33 1.1645052859968743 2207 34-35 1.1106562956573072 2207 36-37 1.1742745382012387 2207 38-39 1.1858908759133513 2207 40-41 1.2144342001371982 2207 42-43 1.3188516108823407 2207 44-45 1.2806741359093294 2207 46-47 1.2731745402751002 2207 48-49 1.3170449208359472 2207 50-51 1.3054316865233346 2207 52-53 1.30338077322628 2207 54-55 1.2814872187631465 2207 56-57 1.2923497131082513 2207 58-59 1.1925516610177667 2207 60-61 1.2141944708975032 2207 62-63 1.3159932515640733 2207 64-65 1.3047666252750076 2207 66-67 1.3142108999499413 2207 68-69 1.3838302172821315 2207 70-71 1.3824011066999944 2207 72-73 1.3669111942260628 2207 74-75 1.3445425736211618 2207 76-77 1.2920199021138217 2207 78-79 1.1713549682191982 2207 80-81 1.1913464027044576 2207 82-83 1.1225909361109068 2207 84-85 1.1904135460173642 2207 86-87 1.1964570856960108 2207 88-89 1.2738131652450662 2207 90-91 1.3324426310184734 2207 92-93 1.3727341541661673 2207 94-95 1.4321371596295158 2207 96-97 1.347065632074603 2207 98-99 1.407986006578163 2207 100 1.5794581959082485 2208 1 0.7437952221889681 2208 2 0.7032350740046027 2208 3 0.6454970713835309 2208 4 0.4645502363412177 2208 5 0.551198312361393 2208 6 0.5584995441060485 2208 7 0.6079253335374233 2208 8 0.615889481092303 2208 9 0.8021414851538893 2208 10-11 0.7750397487035983 2208 12-13 0.8214615199273823 2208 14-15 1.0399157101281276 2208 16-17 0.9972252071436642 2208 18-19 0.9751118510096148 2208 20-21 0.9641533441422538 2208 22-23 1.0387953449089053 2208 24-25 1.0552763579955382 2208 26-27 1.065956774479055 2208 28-29 1.0689428061536148 2208 30-31 1.1204179313210645 2208 32-33 1.1583352252760406 2208 34-35 1.2139891694217582 2208 36-37 1.1808833511471306 2208 38-39 1.2237784477664988 2208 40-41 1.2464171082707054 2208 42-43 1.2965819713027642 2208 44-45 1.3120350805622323 2208 46-47 1.2970606022353977 2208 48-49 1.294086776524857 2208 50-51 1.3321110208692346 2208 52-53 1.3653798157642427 2208 54-55 1.3453872475594508 2208 56-57 1.382731367763114 2208 58-59 1.4029354649417698 2208 60-61 1.4618999426334938 2208 62-63 1.467541239710016 2208 64-65 1.4800954448207975 2208 66-67 1.4711055743183579 2208 68-69 1.4482262950802465 2208 70-71 1.4509651433763437 2208 72-73 1.4682367181310312 2208 74-75 1.451710041044187 2208 76-77 1.385401308040187 2208 78-79 1.2486696819110605 2208 80-81 1.2517726710019836 2208 82-83 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1.1861057508535744 2310 84-85 1.2580886885901528 2310 86-87 1.2925786359678852 2310 88-89 1.3246085612147027 2310 90-91 1.2936032484766784 2310 92-93 1.3584677903535542 2310 94-95 1.4658389529465303 2310 96-97 1.4848410821636975 2310 98-99 1.4558045425024808 2310 100 1.5055890447660012 2311 1 0.3958570879820833 2311 2 0.5077321835779998 2311 3 0.5326707917619373 2311 4 0.39306131468405425 2311 5 0.0042205381824445 2311 6 0.17432262095461937 2311 7 0.4025995707065064 2311 8 0.22560636553068036 2311 9 0.39363444418503946 2311 10-11 0.569509707185297 2311 12-13 0.6215830845111725 2311 14-15 0.6816022781301854 2311 16-17 0.9205845081602746 2311 18-19 0.9455125011397811 2311 20-21 0.9585529364900012 2311 22-23 0.9662688313832462 2311 24-25 0.8350223331061244 2311 26-27 0.8967668701841944 2311 28-29 0.9047343151013507 2311 30-31 0.9434998390260816 2311 32-33 1.1262737276040085 2311 34-35 1.0381530008186601 2311 36-37 1.142434833060321 2311 38-39 1.1477828253351419 2311 40-41 1.1238684628675344 2311 42-43 1.1709480765896885 2311 44-45 1.204097277866687 2311 46-47 1.2436181361162681 2311 48-49 1.239110008028426 2311 50-51 1.2661642486210525 2311 52-53 1.2537181858960835 2311 54-55 1.2512347516612365 2311 56-57 1.2841273478606254 2311 58-59 1.3363270819673616 2311 60-61 1.367368563488995 2311 62-63 1.3834165617895238 2311 64-65 1.35593781960322 2311 66-67 1.425982713722071 2311 68-69 1.4238151943033728 2311 70-71 1.4502801088533985 2311 72-73 1.4180744206087894 2311 74-75 1.289870674092505 2311 76-77 1.1488899080467263 2311 78-79 1.1318790730202934 2311 80-81 1.1626066290848804 2311 82-83 1.2478573275936249 2311 84-85 1.2582379700739885 2311 86-87 1.3266442380110988 2311 88-89 1.3624252694526362 2311 90-91 1.3803283152493862 2311 92-93 1.4198746724074915 2311 94-95 1.5175395330950217 2311 96-97 1.5959836386631352 2311 98-99 1.5331176978456043 2311 100 1.617841718398278 2312 1 0.18533688712814111 2312 2 0.20313404994401907 2312 3 0.3795622464135846 2312 4 0.2983733129453725 2312 5 0.3721850129330804 2312 6 0.48249882546704015 2312 7 0.5289433225459277 2312 8 0.3339111600357185 2312 9 0.5349035096889594 2312 10-11 0.3929158387545968 2312 12-13 0.5423896652398739 2312 14-15 0.9265057496072018 2312 16-17 0.9492318591611522 2312 18-19 0.9747828525436333 2312 20-21 0.9513149227526085 2312 22-23 0.5873910597934895 2312 24-25 0.54073583550813 2312 26-27 0.852360294888868 2312 28-29 1.0228119309342176 2312 30-31 0.9781694895239141 2312 32-33 0.7803388783576608 2312 34-35 0.9622725849839782 2312 36-37 1.0610091820734837 2312 38-39 1.0685548542950585 2312 40-41 1.0922990718135992 2312 42-43 0.9952472928246223 2312 44-45 0.8594850038613302 2312 46-47 1.0660916234859243 2312 48-49 1.1048846178438296 2312 50-51 1.0803051271080406 2312 52-53 1.1053659036501031 2312 54-55 1.1219962987256267 2312 56-57 1.1206604620476384 2312 58-59 1.1384255076455148 2312 60-61 1.2150859733967678 2312 62-63 1.204323252544178 2312 64-65 1.274746071096267 2312 66-67 1.2420421820658447 2312 68-69 1.2920510346887752 2312 70-71 1.0671464477061434 2312 72-73 1.151301653614759 2312 74-75 1.251858965158668 2312 76-77 1.1678832476284384 2312 78-79 1.1008539391101166 2312 80-81 0.9753958139420469 2312 82-83 0.9810973697928809 2312 84-85 0.8333605924042793 2312 86-87 1.0299502459710013 2312 88-89 0.6874730831963731 2312 90-91 1.0260205142741015 2312 92-93 1.2252272444337358 2312 94-95 1.3183249055305808 2312 96-97 1.3482057869310218 2312 98-99 1.383342988418235 2312 100 1.5122882878979986 2313 1 -0.23045796945750752 2313 2 0.03800119563214821 2313 3 0.3180383380298153 2313 4 0.19076167083641593 2313 5 0.3214126469894367 2313 6 0.38722409811833103 2313 7 0.15254127704338316 2313 8 0.12370722990275596 2313 9 0.34872708958803145 2313 10-11 0.42306620476114887 2313 12-13 0.6071809628667921 2313 14-15 0.6916361054581444 2313 16-17 0.8321062216267165 2313 18-19 0.7220386257409004 2313 20-21 0.8560179878608736 2313 22-23 0.8881825146976183 2313 24-25 0.7478953325805264 2313 26-27 0.9195703453523905 2313 28-29 0.8456432443787207 2313 30-31 0.8310706475336005 2313 32-33 0.960318176182291 2313 34-35 1.0396159526037465 2313 36-37 0.8905630315457032 2313 38-39 0.6330116951799383 2313 40-41 0.7897105629364205 2313 42-43 0.8266450738677733 2313 44-45 0.9779639669101741 2313 46-47 0.9699323016652812 2313 48-49 0.981189218876473 2313 50-51 0.937420609027896 2313 52-53 0.9717150500841782 2313 54-55 1.035057034693125 2313 56-57 1.04728275856462 2313 58-59 0.9727742586397312 2313 60-61 1.0168219364051652 2313 62-63 1.043919144463274 2313 64-65 1.1424898889782398 2313 66-67 1.1325480934423808 2313 68-69 1.1262220579343492 2313 70-71 1.0810789218565091 2313 72-73 1.2446436731440969 2313 74-75 0.8843498857696162 2313 76-77 0.9893030924182966 2313 78-79 0.8429549739942033 2313 80-81 0.907415563466845 2313 82-83 0.9758095479924584 2313 84-85 1.0077314296133686 2313 86-87 1.0756156634072056 2313 88-89 1.0617232752992578 2313 90-91 1.1576268449067584 2313 92-93 0.8889327271175169 2313 94-95 0.9469495612058161 2313 96-97 1.2948101403643832 2313 98-99 1.2903117756311495 2313 100 1.3731769221475822 2314 1 -0.2880219883050046 2314 2 -0.10911383345540315 2314 3 0.16937062708018402 2314 4 -0.1039052209738216 2314 5 0.24676187143341366 2314 6 0.23533067628688542 2314 7 0.1635457945925154 2314 8 0.23247140724038218 2314 9 0.28235955227103204 2314 10-11 0.46282110078438876 2314 12-13 0.5733037866273634 2314 14-15 0.7095687832227497 2314 16-17 0.8720364174435744 2314 18-19 0.9474443036206921 2314 20-21 0.5777410471408402 2314 22-23 0.7866338883577697 2314 24-25 0.9489085751216066 2314 26-27 0.9161672109035877 2314 28-29 0.8802715815611606 2314 30-31 0.885276980682427 2314 32-33 0.9905170300384398 2314 34-35 1.006404743023488 2314 36-37 0.9354492206545544 2314 38-39 0.9843681111807072 2314 40-41 1.0539650776007576 2314 42-43 0.8398790579087532 2314 44-45 0.9621978963701778 2314 46-47 1.0683261821255812 2314 48-49 0.8871197480549569 2314 50-51 1.098481081811002 2314 52-53 1.1956848226655765 2314 54-55 1.2340000318017488 2314 56-57 1.1011375098810205 2314 58-59 1.1112160981502797 2314 60-61 1.1657566410636875 2314 62-63 0.9033766408912669 2314 64-65 1.0561770181187384 2314 66-67 1.1618599400767664 2314 68-69 1.2203791167092817 2314 70-71 1.2821349805536357 2314 72-73 1.2518072978829693 2314 74-75 1.1989108352690536 2314 76-77 0.9956490998057745 2314 78-79 0.9959560896477484 2314 80-81 0.9999780666480866 2314 82-83 0.9928845487304692 2314 84-85 0.8511108156743852 2314 86-87 1.0275051704164042 2314 88-89 1.1256236029691706 2314 90-91 1.233060381383737 2314 92-93 1.232489434981371 2314 94-95 1.3050311356595437 2314 96-97 1.3482679274661535 2314 98-99 1.3283620824774651 2314 100 1.3798707549433296 2315 1 -0.043594154168047794 2315 2 0.1356223592373098 2315 3 0.1493433173481833 2315 4 -0.03228255216692588 2315 5 0.27989297578626804 2315 6 0.3955747656633122 2315 7 0.44356243602811674 2315 8 0.4126987514208267 2315 9 0.6308938936710433 2315 10-11 0.6114199552006241 2315 12-13 0.6592373896685118 2315 14-15 0.778881828116063 2315 16-17 0.7639198654840058 2315 18-19 0.8207567707416175 2315 20-21 0.8265605263882492 2315 22-23 0.930093556006355 2315 24-25 0.8211433307330509 2315 26-27 0.912839580932463 2315 28-29 0.9543082096670048 2315 30-31 0.9188027635804232 2315 32-33 0.9342232885532766 2315 34-35 0.8903763194238152 2315 36-37 0.9414988744926376 2315 38-39 0.9503165614675524 2315 40-41 0.9403790955243281 2315 42-43 1.0215867514296448 2315 44-45 0.9247176324591067 2315 46-47 0.935312923624231 2315 48-49 1.0290117995602301 2315 50-51 0.9389807511612531 2315 52-53 0.9932444520265804 2315 54-55 0.8949807550254363 2315 56-57 1.0663769871085549 2315 58-59 1.0624004796536823 2315 60-61 1.0640449406627894 2315 62-63 1.0571987712163775 2315 64-65 1.1095319500398233 2315 66-67 1.0609795462231233 2315 68-69 0.9716402463070324 2315 70-71 0.6099488582969315 2315 72-73 0.8758045186611909 2315 74-75 1.0430522734640064 2315 76-77 1.0254523709900951 2315 78-79 0.8932495131073424 2315 80-81 0.9174139263045582 2315 82-83 0.9244271465168445 2315 84-85 0.8222967051619712 2315 86-87 0.8358683780924565 2315 88-89 0.9325027216077544 2315 90-91 1.032982379518124 2315 92-93 1.0389658336453564 2315 94-95 1.1193494066173209 2315 96-97 1.21913294230216 2315 98-99 1.107505999680832 2315 100 1.0209931551837776 2316 1 0.48820264198792884 2316 2 0.4776151323928488 2316 3 0.511664658218244 2316 4 0.31581283138348937 2316 5 0.41657930947895494 2316 6 0.42831320573579035 2316 7 0.5036037832436762 2316 8 0.5144647608738353 2316 9 0.5933926714392399 2316 10-11 0.5418389033845656 2316 12-13 0.6574737698801485 2316 14-15 0.8550477641943388 2316 16-17 0.84502525988119 2316 18-19 0.8422950846948183 2316 20-21 0.8892381249970498 2316 22-23 0.9805482821118972 2316 24-25 0.986713377713599 2316 26-27 1.0346213140350358 2316 28-29 0.9683360955921572 2316 30-31 0.8409763587058237 2316 32-33 0.7977423284857323 2316 34-35 0.7342651582414916 2316 36-37 0.7265357572043314 2316 38-39 0.6916834616809595 2316 40-41 0.7861692392149422 2316 42-43 0.9149625107660029 2316 44-45 0.8805791854325378 2316 46-47 0.9098723947484686 2316 48-49 0.6260609419392367 2316 50-51 0.7319617301613022 2316 52-53 0.9123875268794279 2316 54-55 0.9686179832155872 2316 56-57 1.021551786564764 2316 58-59 0.9327082388045369 2316 60-61 1.017112262200726 2316 62-63 1.0405639846531898 2316 64-65 1.0143632631394865 2316 66-67 0.9754366606179588 2316 68-69 0.9695352955880594 2316 70-71 0.9209682724740844 2316 72-73 0.977407665591663 2316 74-75 1.001866343070212 2316 76-77 0.7689727458683109 2316 78-79 0.7693848735124007 2316 80-81 0.7565523481880767 2316 82-83 0.7178955870195445 2316 84-85 0.7320485182877796 2316 86-87 0.749506572463563 2316 88-89 0.6369857281909788 2316 90-91 0.7500808426843868 2316 92-93 0.7899253893018248 2316 94-95 0.6143993194482853 2316 96-97 0.8319449187168573 2316 98-99 0.9618803818225068 2316 100 0.628865656087747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 16.0 12 51.0 13 280.0 14 1097.0 15 3093.0 16 7295.0 17 14918.0 18 24269.0 19 34025.0 20 45353.0 21 59664.0 22 71931.0 23 76811.0 24 79956.0 25 87585.0 26 103455.0 27 125437.0 28 153715.0 29 186590.0 30 227642.0 31 281768.0 32 354335.0 33 460730.0 34 640461.0 35 854189.0 36 1256202.0 37 1865489.0 38 1575118.0 39 217668.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60209027400532 19.83753418493318 13.58612229423819 23.97425324682331 2 15.69470868959129 20.967890241521324 40.59481267226387 22.742588396623514 3 17.455909530534125 28.066455825527065 31.265035485789795 23.21259915814902 4 12.245776615638244 17.600229803883277 38.39078241182809 31.76321116865039 5 12.322683562444402 38.24226105901943 34.17183307472912 15.263222303807044 6 28.823129293401255 37.47868979078363 19.791312631695334 13.906868284119774 7 26.299430875527648 33.40059676620149 23.38752512303975 16.912447235231117 8 26.181133065868284 35.410255228498265 20.845620780086467 17.562990925546984 9 28.144966126619398 15.452500640823196 20.38874639544047 36.01378683711693 10-11 24.913403405242548 25.84582057166268 29.338529554715247 19.902246468379523 12-13 25.239095552514517 24.097706528507054 28.655208485282607 22.00798943369582 14-15 23.05309690813775 25.399808403914243 26.674657972380334 24.872436715567677 16-17 22.169839191645096 27.506880487511253 27.47721814946856 22.846062171375095 18-19 21.849483946255944 27.044383346425764 29.202704911705542 21.90342779561275 20-21 22.87531387782717 26.656934372857343 28.418702804807722 22.049048944507764 22-23 22.521641682440023 26.41313732822138 28.584326674581156 22.48089431475744 24-25 22.015251293413005 26.234753857478605 28.928843284562667 22.821151564545726 26-27 22.087043486552634 26.365567205045657 28.723096186242504 22.824293122159204 28-29 22.06625937470414 27.493643489137003 28.44067790068382 21.99941923547504 30-31 23.43414878904812 26.384972555314356 28.644878737371343 21.535999918266178 32-33 23.146041238972092 26.46662387166404 28.398439034492064 21.988895854871807 34-35 23.017697212916907 26.81421100015635 28.519166312879857 21.648925474046884 36-37 22.54050635800527 26.804939829945233 28.558868793659016 22.095685018390483 38-39 22.62028806210095 26.855620490052782 28.483172609382386 22.040918838463885 40-41 22.633461083208907 26.14241842649584 29.04213362006579 22.18198687022946 42-43 22.373700181597975 26.677397335617055 28.22884704868953 22.720055434095443 44-45 21.52417351835858 27.120711347957148 28.79808045720267 22.5570346764816 46-47 21.627319333435928 26.291062995337562 28.380409483687203 23.701208187539308 48-49 22.062018150133582 26.463617683231305 28.396611053248467 23.077753113386652 50-51 21.950634003648503 26.854872004086616 28.300468099891813 22.894025892373072 52-53 21.783747433798766 27.03556724826704 27.92411265240244 23.256572665531756 54-55 21.569192869767683 26.590017204747625 28.558303000757977 23.282486924726715 56-57 22.10391189224574 26.538179502753778 27.928410548178263 23.429498056822222 58-59 21.497112891524157 26.781165958941035 28.463160736528124 23.25856041300668 60-61 22.233415951493697 27.028347543895308 27.927836753765284 22.81039975084571 62-63 22.23851980002452 26.921080294423483 27.672906818873255 23.167493086678746 64-65 21.64300223451946 26.506404523015732 27.746752719972946 24.103840522491858 66-67 21.542854647585038 26.478033181099832 27.62247886155303 24.356633309762096 68-69 21.103421611953806 26.72814681909268 27.94358453579642 24.224847033157097 70-71 21.450047195091937 26.463743482210727 28.03540648370046 24.050802838996876 72-73 21.45074102067438 27.096377857487887 27.26138539213166 24.191495729706077 74-75 21.75969067111965 27.129933265986715 26.96614847470587 24.14422758818776 76-77 21.517110521972477 26.803353264311568 27.212828271387195 24.466707942328757 78-79 21.267131790884154 26.843961924648337 27.533536411260844 24.355369873206666 80-81 21.291404247561115 27.173881393479576 27.073722288874503 24.460992070084806 82-83 21.784410745170995 27.031326077714635 27.142291669694636 24.041971507419735 84-85 21.828821767407664 27.430475286117893 26.46573152547016 24.27497142100428 86-87 21.294293003046242 27.574541626944676 26.71461017478131 24.416555195227772 88-89 21.24764664891324 27.020837841260754 26.870388070788803 24.861127439037205 90-91 21.91768571763078 26.655629684296805 26.91202433390025 24.514660264172168 92-93 21.645277445211317 27.121295870705424 26.625186444126715 24.608240239956544 94-95 21.13613597789284 26.874390613346726 26.73513386057489 25.254339548185545 96-97 21.06846144825451 26.750974054686466 26.930289118934176 25.250275378124844 98-99 21.046291953773842 26.891449968908887 26.69792033850569 25.36433773881158 100 20.87278609880496 27.047917149447976 26.84926766167776 25.230029090069305 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1539.0 1 1153.0 2 1989.0 3 3031.0 4 2152.5 5 1565.0 6 2096.5 7 3865.0 8 5946.5 9 6860.5 10 6479.5 11 5948.5 12 6348.0 13 7150.0 14 8179.0 15 9310.5 16 10317.0 17 11347.5 18 12515.5 19 13723.5 20 15018.0 21 16647.0 22 18969.0 23 22540.5 24 27367.0 25 33179.5 26 40694.0 27 49771.5 28 60803.5 29 74996.0 30 92113.5 31 108701.0 32 129205.5 33 149403.5 34 166557.0 35 184976.0 36 205108.5 37 230821.5 38 245456.5 39 244946.5 40 244517.0 41 246720.0 42 252790.0 43 263282.0 44 276842.0 45 300006.0 46 321236.5 47 338474.5 48 362272.5 49 382079.0 50 396076.5 51 403140.5 52 391014.0 53 366565.5 54 342397.0 55 307214.5 56 268976.0 57 233324.0 58 197116.0 59 163370.5 60 127023.0 61 96270.0 62 75247.0 63 57910.5 64 41878.5 65 29651.0 66 21531.5 67 16309.5 68 12350.0 69 9071.0 70 6951.5 71 5282.0 72 3953.0 73 3002.0 74 2317.5 75 1695.5 76 1179.0 77 737.5 78 483.0 79 317.5 80 196.0 81 124.5 82 87.0 83 52.5 84 32.5 85 22.5 86 13.0 87 9.0 88 6.5 89 4.0 90 3.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050822098815324054 2 0.0 3 0.0 4 0.018730503248890928 5 0.0037688042900798713 6 2.2703640301685973E-5 7 1.702773022626448E-4 8 3.859618851286615E-4 9 0.002020623986850052 10-11 7.548960400310587E-4 12-13 2.8379550377107465E-4 14-15 3.405546045252896E-5 16-17 1.0784229143300837E-4 18-19 0.0 20-21 0.0 22-23 2.2703640301685973E-5 24-25 6.243501082963642E-5 26-27 0.0 28-29 9.308492523691249E-4 30-31 8.797660616903314E-4 32-33 0.014507626152777337 34-35 0.02346421225179245 36-37 0.030683969867728596 38-39 0.020694368134986763 40-41 2.1568458286601674E-4 42-43 0.001600606641268861 44-45 0.009223353872559927 46-47 0.013786785573198807 48-49 0.005272920460066567 50-51 0.010108795844325679 52-53 0.015824437290275124 54-55 0.009484445736029315 56-57 0.0030025564298979696 58-59 0.008848743807582108 60-61 0.0018219671342102992 62-63 9.081456120674389E-4 64-65 0.018997271022435737 66-67 0.04468644002379341 68-69 0.05450008854419717 70-71 0.04789332921640656 72-73 0.03974839825817671 74-75 0.02816954170431687 76-77 0.028578207229747216 78-79 0.011885355697932606 80-81 0.001992244436472944 82-83 0.026971924678402932 84-85 0.04744493232044826 86-87 0.047836570115652344 88-89 0.059313260288154604 90-91 0.03415762683388655 92-93 0.025337262576681546 94-95 0.006930286202089643 96-97 0.004120710714756004 98-99 0.022493631628895377 100 0.019150520594472118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8809160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.85443418136745 #Duplication Level Percentage of deduplicated Percentage of total 1 75.79017573994142 43.84797733940714 2 14.264995026804073 16.505864317515407 3 4.526220561064775 7.855857886214225 4 1.9133460988286781 4.427822237634397 5 1.0069212411479809 2.912742933590835 6 0.5829907247700378 2.0237159108733516 7 0.39027509976337915 1.5805401550320926 8 0.26965262527185835 1.2480480048498939 9 0.19949419708246297 1.038746150520489 >10 0.9726042039915823 9.87494666333832 >50 0.0522805176404426 2.066907350318771 >100 0.026995570288597955 2.931322123133263 >500 0.0026735180483031906 1.0671126677542497 >1k 0.0011711901195991079 1.198265616204901 >5k 1.273032730209417E-4 0.4521262314598905 >10k+ 7.638196381256504E-5 0.968004412152804 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27889 0.31659091218686003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21498 0.24404142960282252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16329 0.18536387124311512 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.2703640301685973E-5 0.0 2 0.0 0.0 0.0 2.2703640301685973E-5 0.0 3 0.0 0.0 0.0 2.2703640301685973E-5 0.0 4 0.0 0.0 0.0 2.2703640301685973E-5 0.0 5 0.0 0.0 0.0 2.2703640301685973E-5 0.0 6 0.0 0.0 0.0 3.405546045252896E-5 1.1351820150842987E-5 7 0.0 0.0 0.0 3.405546045252896E-5 1.1351820150842987E-5 8 0.0 0.0 0.0 4.5407280603371946E-5 2.2703640301685973E-5 9 0.0 0.0 0.0 4.5407280603371946E-5 3.405546045252896E-5 10-11 0.0 0.0 0.0 1.2487002165927286E-4 3.405546045252896E-5 12-13 0.0 0.0 0.0 1.8730503248890926E-4 5.675910075421493E-5 14-15 0.0 5.675910075421493E-6 0.0 2.2703640301685973E-4 6.811092090505792E-5 16-17 0.0 1.1351820150842987E-5 0.0 3.235268742990251E-4 1.3054593173469437E-4 18-19 0.0 1.1351820150842987E-5 0.0 4.427209858828765E-4 1.702773022626448E-4 20-21 0.0 1.1351820150842987E-5 0.0 6.867851191260006E-4 1.9298094256433077E-4 22-23 0.0 1.1351820150842987E-5 0.0 0.0017595321233806627 2.383882231677027E-4 24-25 0.0 1.702773022626448E-5 0.0 0.004069627524077211 2.8379550377107465E-4 26-27 0.0 2.2703640301685973E-5 0.0 0.007094887594276867 2.8947141384649616E-4 28-29 0.0 2.2703640301685973E-5 0.0 0.01616499189480041 2.9514732392191766E-4 30-31 0.0 2.2703640301685973E-5 0.0 0.05159969849565679 3.121750541481821E-4 32-33 0.0 2.2703640301685973E-5 0.0 0.13579614855445923 3.7461006497781853E-4 34-35 0.0 3.9731370527950454E-5 0.0 0.2450687693264738 3.973137052795045E-4 36-37 0.0 5.108319067879344E-5 0.0 0.40905148731547614 3.973137052795045E-4 38-39 0.0 6.811092090505792E-5 0.0 0.7069005444332945 4.086655254303475E-4 40-41 0.0 9.081456120674389E-5 0.0 1.101586303347879 4.1434143550576896E-4 42-43 0.0 1.2487002165927286E-4 0.0 1.4586351025523432 4.2001734558119047E-4 44-45 0.0 1.3054593173469437E-4 0.0 1.8315537463276863 4.37045075807455E-4 46-47 0.0 1.3622184181011585E-4 0.0 2.2461562736969247 4.597487161091409E-4 48-49 0.0 1.4757366196095883E-4 0.0 2.709719201376749 4.8812826648624843E-4 50-51 0.0 1.5892548211180182E-4 0.0 3.228713066853139 5.108319067879343E-4 52-53 0.0 1.5892548211180182E-4 0.0 3.7749967079721563 5.335355470896204E-4 54-55 0.0 1.5892548211180182E-4 0.0 4.338750800303321 5.448873672404634E-4 56-57 0.0 1.5892548211180182E-4 0.0 4.895818670565639 5.448873672404634E-4 58-59 0.0 1.5892548211180182E-4 0.0 5.546283641119017 5.448873672404634E-4 60-61 0.0 1.646013921872233E-4 0.0 6.212045189325657 5.448873672404634E-4 62-63 0.0 1.702773022626448E-4 0.0 6.923639711391324 5.562391873913063E-4 64-65 0.0 1.702773022626448E-4 0.0 7.559875175385621 5.562391873913063E-4 66-67 5.675910075421493E-6 2.0433276271517376E-4 0.0 8.24399829268625 5.619150974667278E-4 68-69 1.1351820150842987E-5 2.0433276271517376E-4 0.0 9.011154298480218 5.732669176175708E-4 70-71 1.1351820150842987E-5 2.0433276271517376E-4 0.0 9.807234742018537 5.902946478438353E-4 72-73 1.1351820150842987E-5 2.0433276271517376E-4 0.0 10.52984052963052 6.470537485980502E-4 74-75 1.1351820150842987E-5 2.0433276271517376E-4 0.0 11.245340077828079 6.584055687488932E-4 76-77 1.1351820150842987E-5 2.1568458286601674E-4 0.0 12.001291837133166 6.811092090505792E-4 78-79 1.1351820150842987E-5 2.1568458286601674E-4 0.0 12.80089702082832 6.811092090505792E-4 80-81 1.1351820150842987E-5 2.1568458286601674E-4 0.0 13.569733096004612 6.981369392768436E-4 82-83 1.1351820150842987E-5 2.1568458286601674E-4 0.0 14.368396078627248 7.265164896539511E-4 84-85 1.1351820150842987E-5 2.1568458286601674E-4 0.0 15.15574129655949 7.265164896539511E-4 86-87 1.1351820150842987E-5 2.1568458286601674E-4 0.0 15.931422519286743 7.321923997293726E-4 88 1.1351820150842987E-5 2.1568458286601674E-4 0.0 16.50606868305264 7.378683098047942E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 9525 0.0 50.274933 2 GGTATCA 17010 0.0 43.31136 1 ACCTGGG 11105 0.0 42.40346 3 GTATCAA 28160 0.0 37.498528 1 CCTGGGG 10290 0.0 34.253536 4 TAACGCA 1105 0.0 33.598843 4 GTACCTG 15620 0.0 31.002256 1 CTGGGGG 7155 0.0 30.86501 5 GTACATG 32760 0.0 29.764729 1 TGGGGGG 21215 0.0 29.566948 6 ATCAACG 35425 0.0 29.278217 3 CAACGCA 35770 0.0 29.043436 5 TATCAAC 36190 0.0 28.970318 2 TACATGG 33190 0.0 28.728771 2 ATAACGC 1295 0.0 28.668797 3 TCAACGC 36825 0.0 28.25494 4 ATGGGGG 20400 0.0 27.823704 5 AACGCAG 38020 0.0 27.707146 6 ACATGGG 33310 0.0 27.525473 3 CATGGGG 25910 0.0 27.00765 4 >>END_MODULE