FastQCFastQC Report
Mon 6 Jun 2016
ERR1211377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9974706
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282220.282935657451959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211160.2116954625028547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145210.14557822556374092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146650.050.349841
GTATCAA276100.043.7926641
CAACGCA339000.034.1547245
ATCAACG345000.033.656113
TACCTGG67600.033.0037042
TATCAAC362700.032.806552
AACGCAG370100.031.86916
TCAACGC370150.031.5344334
GTACTAG52700.030.3109991
ACGCAGA403100.028.5956697
CGCAGAG411400.027.9140878
GTACATG388850.027.8106881
TACATGG394850.026.8929212
GCATCGT53450.026.3838278
ACATGGG394700.025.9284973
TAGGCAT68550.025.510655
ACCTGGG83900.025.3817943
GCAGAGT451050.024.6369579
GCTATTG84800.024.6122538
ATTTAGG88300.024.3874651
Produced by FastQC (version 0.11.5)