FastQCFastQC Report
Mon 6 Jun 2016
ERR1211377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9974706
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249070.24970159521493668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198480.1989833083802169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139520.13987379678157932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153900.049.9510841
GTATCAA279100.041.4083371
CAACGCA349450.032.3379675
ATCAACG353250.031.8886413
TATCAAC372400.030.9044152
TACCTGG64500.030.6006762
AACGCAG380800.030.2046076
TCAACGC380000.029.7679654
GTACTAG50800.027.951751
ACGCAGA418450.027.0265887
CGCAGAG427250.026.3379618
GTACATG382550.026.1423761
TACATGG390800.025.3247552
ACATGGG385950.024.7181023
ACCTGGG80300.024.4630643
GCAGAGT468650.023.6503759
ATTTAGG87800.023.4555361
TAGGCAT67550.022.6764415
GGGCTAT91600.022.6204786
TAGTACT67650.022.2994354
Produced by FastQC (version 0.11.5)