FastQCFastQC Report
Mon 6 Jun 2016
ERR1211376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602734
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170210.3698019481464712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126100.2739676027335058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88750.19282018035367676No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49260.10702334742785484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73400.053.1379931
GTATCAA140200.044.518321
TACCTGG40250.043.4961432
ACCTGGG45000.038.130063
ATCAACG180350.033.859473
CAACGCA183050.033.359685
TATCAAC185300.033.347482
TCAACGC189800.032.19844
GTACATG192800.031.9818461
AACGCAG194750.031.8631236
TACATGG195700.030.998152
ACATGGG195450.029.6801873
ATGGGGG127950.029.428945
CATGGGG154450.029.2802184
CCTGGGG44300.029.182074
TGGGGGG135400.029.0949256
ACGCAGA214450.028.4982787
CGCAGAG216950.028.0810688
TAACGCA5100.027.6527424
GTGGTTA38650.026.691041
Produced by FastQC (version 0.11.5)