##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211376_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4602734 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.349137056366935 31.0 31.0 34.0 28.0 34.0 2 31.403152343802617 31.0 31.0 34.0 28.0 34.0 3 31.465544391659392 31.0 31.0 34.0 28.0 34.0 4 35.09891859924993 37.0 35.0 37.0 32.0 37.0 5 35.15475128477987 37.0 35.0 37.0 32.0 37.0 6 35.21589885489798 37.0 35.0 37.0 32.0 37.0 7 35.14812326760573 37.0 35.0 37.0 32.0 37.0 8 35.146628938365765 37.0 35.0 37.0 32.0 37.0 9 36.707551859394876 39.0 37.0 39.0 32.0 39.0 10-11 36.62494063311067 39.0 37.0 39.0 32.0 39.0 12-13 36.525950011449716 39.0 37.0 39.0 32.0 39.0 14-15 37.700591539724 40.0 37.0 41.0 32.0 41.0 16-17 37.68533647610312 40.0 37.0 41.0 32.0 41.0 18-19 37.68992125115203 40.0 37.0 41.0 32.0 41.0 20-21 37.6784330791221 40.0 37.0 41.0 32.0 41.0 22-23 37.561426317488696 40.0 37.0 41.0 32.0 41.0 24-25 37.46979708147375 40.0 37.0 41.0 31.5 41.0 26-27 37.335244987001204 40.0 36.5 41.0 31.0 41.0 28-29 37.19155071746488 40.0 36.0 41.0 31.0 41.0 30-31 37.0196917744975 39.0 36.0 41.0 30.5 41.0 32-33 36.79236177889055 39.0 35.5 41.0 30.0 41.0 34-35 36.67778846224874 39.0 35.0 41.0 30.0 41.0 36-37 36.56139068649198 39.0 35.0 41.0 30.0 41.0 38-39 36.407051113533825 39.0 35.0 41.0 29.5 41.0 40-41 36.24195000189018 39.0 35.0 40.0 29.0 41.0 42-43 36.05811850087361 39.0 35.0 40.0 28.0 41.0 44-45 35.928799709042494 39.0 35.0 40.0 27.5 41.0 46-47 35.71396511290898 38.0 35.0 40.0 26.5 41.0 48-49 35.47384033055136 38.0 34.0 40.0 26.0 41.0 50-51 34.810020196691795 37.5 33.5 39.5 25.0 40.5 52-53 35.12491749468903 38.0 34.0 40.0 26.0 40.5 54-55 35.54078021888729 38.5 34.0 40.0 26.0 41.0 56-57 35.47611256700909 38.0 34.0 40.0 26.0 41.0 58-59 35.289731385737255 38.0 34.0 40.0 26.0 41.0 60-61 35.063696272693576 38.0 34.0 40.0 26.0 41.0 62-63 34.76885824816294 37.0 34.0 40.0 25.5 41.0 64-65 34.56420281945469 37.0 33.5 40.0 25.5 41.0 66-67 34.36833999097058 36.5 33.0 40.0 25.0 41.0 68-69 34.03957973673907 36.0 33.0 39.0 24.5 41.0 70-71 33.67210825131325 35.5 33.0 39.0 24.0 41.0 72-73 33.2704318780968 35.0 32.0 38.5 23.0 40.0 74-75 32.87253651851269 35.0 32.0 37.5 22.5 40.0 76-77 32.4824029587632 35.0 32.0 37.0 21.5 39.0 78-79 32.10949068531877 35.0 31.0 36.5 20.0 39.0 80-81 31.757819374311005 35.0 31.0 36.0 20.0 38.5 82-83 31.40627407536477 35.0 31.0 35.5 19.5 37.0 84-85 31.081235304929635 34.5 30.5 35.0 18.5 37.0 86-87 30.751995878971066 34.0 30.0 35.0 18.0 36.5 88-89 30.47454306505655 34.0 30.0 35.0 17.0 36.0 90-91 30.208512809995103 34.0 29.0 35.0 16.0 36.0 92-93 29.95181255314776 34.0 29.0 35.0 15.0 35.5 94-95 29.685722876881435 34.0 29.0 35.0 11.5 35.0 96-97 29.409205159368323 34.0 28.5 35.0 10.0 35.0 98-99 29.135254068560123 34.0 27.0 35.0 10.0 35.0 100 27.93416087047394 33.0 25.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3178823774086901 1101 2 -0.26502555932512806 1101 3 -0.37021809924023685 1101 4 -0.34299070575760027 1101 5 -0.25476785688749715 1101 6 -0.3064341279912739 1101 7 -0.2796124059859437 1101 8 -0.39114377316079185 1101 9 -0.4712396530640035 1101 10-11 -0.43097171289934266 1101 12-13 -0.3959963718639159 1101 14-15 -0.6109070928097253 1101 16-17 -0.6623525394374639 1101 18-19 -0.6788558537245422 1101 20-21 -0.6687910569086455 1101 22-23 -0.6150306803655923 1101 24-25 -0.7472839521213714 1101 26-27 -0.669670297571443 1101 28-29 -0.7468979940088118 1101 30-31 -0.7614293077635423 1101 32-33 -0.6790314439453482 1101 34-35 -0.7620183959188154 1101 36-37 -0.776571025553423 1101 38-39 -0.7298794250455174 1101 40-41 -0.8433079003912383 1101 42-43 -0.8300379523021562 1101 44-45 -0.8523045595635708 1101 46-47 -0.9024337704477645 1101 48-49 -0.8645838247078288 1101 50-51 -0.8645073521419704 1101 52-53 -0.5070903142444578 1101 54-55 -0.6046298785128741 1101 56-57 -0.730486557444209 1101 58-59 -0.6456508792062507 1101 60-61 -0.6217202471440046 1101 62-63 -0.7586649718052527 1101 64-65 -0.7268540597721724 1101 66-67 -0.783083038081962 1101 68-69 -0.7512294187903379 1101 70-71 -0.7163408485765927 1101 72-73 -0.8297888893131642 1101 74-75 -0.9213735894959463 1101 76-77 -0.9771796107805208 1101 78-79 -0.908378796179143 1101 80-81 -1.039346505513329 1101 82-83 -1.0579474581711565 1101 84-85 -1.249928123605244 1101 86-87 -1.255244535077722 1101 88-89 -1.4281365489954148 1101 90-91 -1.4266188496182863 1101 92-93 -1.4436654320469486 1101 94-95 -1.55824211510107 1101 96-97 -1.7623904303131468 1101 98-99 -1.8698682237546116 1101 100 -1.6607422835840602 1102 1 -0.35733430275714184 1102 2 -0.3533789729797441 1102 3 -0.43069530216960317 1102 4 -0.30897227910983815 1102 5 -0.294456021309891 1102 6 -0.3387988811214413 1102 7 -0.3429244778026259 1102 8 -0.38263916649884777 1102 9 -0.5021869717505183 1102 10-11 -0.43994879770917095 1102 12-13 -0.6325898044176625 1102 14-15 -0.7213488598779989 1102 16-17 -0.721294188386203 1102 18-19 -0.7118112045395648 1102 20-21 -0.7324574873362408 1102 22-23 -0.7400011504813548 1102 24-25 -0.7625213723906867 1102 26-27 -0.8016098203685118 1102 28-29 -0.8321802997021805 1102 30-31 -0.7448925725875455 1102 32-33 -0.7659674231563045 1102 34-35 -0.9264407913830226 1102 36-37 -0.7841306759195916 1102 38-39 -0.8715047498742479 1102 40-41 -1.0535606762003553 1102 42-43 -1.1051383539085862 1102 44-45 -1.0848819278602804 1102 46-47 -1.153555906048986 1102 48-49 -1.2316993456149845 1102 50-51 -0.9988092656784744 1102 52-53 -0.9927978502709252 1102 54-55 -0.9182372491719732 1102 56-57 -0.9288092600192144 1102 58-59 -0.9427215146893602 1102 60-61 -0.9737164673188161 1102 62-63 -0.8598934149897559 1102 64-65 -0.9588408303840339 1102 66-67 -1.0884220411530663 1102 68-69 -1.1009813677626994 1102 70-71 -0.9698253749172494 1102 72-73 -0.9065665883445746 1102 74-75 -1.0658337832119855 1102 76-77 -1.0186395682574911 1102 78-79 -1.0934721106133516 1102 80-81 -1.1183684757472037 1102 82-83 -1.2549708458394768 1102 84-85 -1.1856710954927934 1102 86-87 -1.356118619651312 1102 88-89 -1.4469175553738687 1102 90-91 -1.3889387173244039 1102 92-93 -1.4366963793656318 1102 94-95 -1.5075688337403328 1102 96-97 -1.6883294806320706 1102 98-99 -1.7294240942955987 1102 100 -1.6588523709925163 1103 1 -0.42406351828671873 1103 2 -0.5068116156458871 1103 3 -0.5247995133683112 1103 4 -0.36205444535870157 1103 5 -0.31191461481390803 1103 6 -0.34097220469290335 1103 7 -0.48362694122257466 1103 8 -0.44811886872592055 1103 9 -0.6503645014293724 1103 10-11 -0.6392741201684586 1103 12-13 -0.6354559585805504 1103 14-15 -0.7808125988067616 1103 16-17 -0.8178740842295511 1103 18-19 -0.8338078926379993 1103 20-21 -0.7055501441832348 1103 22-23 -0.7881967139256361 1103 24-25 -0.8258144670038092 1103 26-27 -0.8836748665229663 1103 28-29 -0.8717077272104845 1103 30-31 -0.8941348318268822 1103 32-33 -0.9984007273677733 1103 34-35 -0.9476001564621797 1103 36-37 -0.8756170361087641 1103 38-39 -1.0138257789852219 1103 40-41 -1.0340623315276005 1103 42-43 -0.9428608975413795 1103 44-45 -0.9238557198541884 1103 46-47 -1.027553021839374 1103 48-49 -1.002650978090962 1103 50-51 -1.0229588687214601 1103 52-53 -0.7994493167397465 1103 54-55 -0.8593618577739264 1103 56-57 -0.8865354516127368 1103 58-59 -0.718735560205694 1103 60-61 -0.7491852977770819 1103 62-63 -0.643104917387312 1103 64-65 -0.6615071708596787 1103 66-67 -0.6589246567268177 1103 68-69 -0.7177504055984159 1103 70-71 -0.753007055994928 1103 72-73 -0.7901669553311663 1103 74-75 -0.8462929946679694 1103 76-77 -0.9606737813360269 1103 78-79 -0.9370011092172277 1103 80-81 -0.8781839056342733 1103 82-83 -0.9512522790694611 1103 84-85 -0.9521340771227003 1103 86-87 -0.994123392255343 1103 88-89 -1.0535051879520019 1103 90-91 -1.1726965734122459 1103 92-93 -1.2493837894170774 1103 94-95 -1.207484618333023 1103 96-97 -1.2538058754036676 1103 98-99 -1.214593870593955 1103 100 -1.4112671523688967 1104 1 -0.4922670946296286 1104 2 -0.47585570965362933 1104 3 -0.4449338178503872 1104 4 -0.287096996450785 1104 5 -0.30253054173679317 1104 6 -0.33002675537647264 1104 7 -0.3826947036342929 1104 8 -0.43094016752581155 1104 9 -0.5373574805801482 1104 10-11 -0.46630403882748084 1104 12-13 -0.5506402642393482 1104 14-15 -0.7163968955267705 1104 16-17 -0.7193172695882168 1104 18-19 -0.8106911468643361 1104 20-21 -0.717571758820938 1104 22-23 -0.7062206019133299 1104 24-25 -0.647895223503177 1104 26-27 -0.7389184317161295 1104 28-29 -0.9375457046566211 1104 30-31 -0.8937423016459931 1104 32-33 -0.8287974883676341 1104 34-35 -0.7979806278913628 1104 36-37 -0.9323512767668944 1104 38-39 -0.9382290650976159 1104 40-41 -0.8697920673729271 1104 42-43 -0.9336093681742668 1104 44-45 -1.0291629254603336 1104 46-47 -0.8457559774318568 1104 48-49 -0.7954420037023695 1104 50-51 -0.7750183297028173 1104 52-53 -0.7020770288523295 1104 54-55 -0.5985672238212842 1104 56-57 -0.6317212621864812 1104 58-59 -0.6884507933779105 1104 60-61 -0.6831731863425503 1104 62-63 -0.7036786883392523 1104 64-65 -0.790087848226861 1104 66-67 -0.7869830940637286 1104 68-69 -0.7851963695439323 1104 70-71 -0.7384686492485955 1104 72-73 -0.7517601010373767 1104 74-75 -0.839301401679883 1104 76-77 -0.8651101442696891 1104 78-79 -0.8702031007573154 1104 80-81 -0.8461612805228356 1104 82-83 -0.8758338285100145 1104 84-85 -1.0371102759321076 1104 86-87 -1.093269730875022 1104 88-89 -1.1488654887708982 1104 90-91 -1.1573092499779563 1104 92-93 -1.1558754030248792 1104 94-95 -1.2321778002910584 1104 96-97 -1.2971460762158884 1104 98-99 -1.2654067999508278 1104 100 -1.2997521996247379 1105 1 -0.42594293799648497 1105 2 -0.427051614496996 1105 3 -0.4279145251536782 1105 4 -0.26352092088676926 1105 5 -0.363653602780559 1105 6 -0.37396444529397854 1105 7 -0.3964413726324878 1105 8 -0.40728253510885537 1105 9 -0.5270352830364047 1105 10-11 -0.5260390432012585 1105 12-13 -0.512694900754525 1105 14-15 -0.6379049739136278 1105 16-17 -0.6438334644182149 1105 18-19 -0.7349348027330151 1105 20-21 -0.7252107197316491 1105 22-23 -0.6476029210344549 1105 24-25 -0.6779314588949106 1105 26-27 -0.7457357598899037 1105 28-29 -0.7210879953077765 1105 30-31 -0.8463881180759145 1105 32-33 -0.8575985659746053 1105 34-35 -0.8816485797028477 1105 36-37 -0.8820282997703544 1105 38-39 -0.8737247636609027 1105 40-41 -0.9217834560904592 1105 42-43 -0.9219066066258037 1105 44-45 -0.9006866521977628 1105 46-47 -0.916394265188579 1105 48-49 -0.981489949429168 1105 50-51 -0.9714116786740803 1105 52-53 -0.8043611004809108 1105 54-55 -0.8013958289414305 1105 56-57 -0.7461565502465817 1105 58-59 -0.7430015741351923 1105 60-61 -0.775630843081025 1105 62-63 -0.8864655188857782 1105 64-65 -0.8391211973792423 1105 66-67 -0.7541048476104635 1105 68-69 -0.8494123650798215 1105 70-71 -0.8399926465844487 1105 72-73 -0.7893327448528851 1105 74-75 -0.919217630447875 1105 76-77 -0.9662019118465643 1105 78-79 -0.9395259136698826 1105 80-81 -0.842034600791262 1105 82-83 -0.8430165206041664 1105 84-85 -0.8477881971929335 1105 86-87 -0.8713923718983025 1105 88-89 -0.8909895925349325 1105 90-91 -0.821813195647831 1105 92-93 -0.8949565952707133 1105 94-95 -0.9679214683420518 1105 96-97 -0.9890335033941895 1105 98-99 -1.033714965834772 1105 100 -1.090264717334918 1106 1 -0.3851515365466831 1106 2 -0.39369169652469793 1106 3 -0.48724407791775803 1106 4 -0.28889875011223154 1106 5 -0.2681153645652614 1106 6 -0.26707994531717816 1106 7 -0.40963832601786265 1106 8 -0.3540462866053815 1106 9 -0.5819035776344919 1106 10-11 -0.6476123866412493 1106 12-13 -0.5537749989537417 1106 14-15 -0.7135487459663636 1106 16-17 -0.6339407319898669 1106 18-19 -0.7486543944761124 1106 20-21 -0.7811677147652176 1106 22-23 -0.6727590776607357 1106 24-25 -0.7165878652410598 1106 26-27 -0.7009741285210112 1106 28-29 -0.6362650488961279 1106 30-31 -0.7210669673864416 1106 32-33 -0.8248292213100683 1106 34-35 -0.7850433395179763 1106 36-37 -0.7423259684335974 1106 38-39 -0.858402592875656 1106 40-41 -0.7790929513284226 1106 42-43 -0.7429996746142322 1106 44-45 -0.7540857624213615 1106 46-47 -0.7647497947131257 1106 48-49 -0.650937555777638 1106 50-51 -0.7889761207068702 1106 52-53 -0.6180959240113069 1106 54-55 -0.6602445490587598 1106 56-57 -0.6663733270289427 1106 58-59 -0.5653174176477833 1106 60-61 -0.6176529922321023 1106 62-63 -0.6588325922084906 1106 64-65 -0.630555409184339 1106 66-67 -0.69044551331082 1106 68-69 -0.5926978417769035 1106 70-71 -0.7237709484828585 1106 72-73 -0.7151137047865888 1106 74-75 -0.6557466066863995 1106 76-77 -0.7168714032784571 1106 78-79 -0.6871669957557671 1106 80-81 -0.8454713431196765 1106 82-83 -0.7585561796671563 1106 84-85 -0.6885422451355012 1106 86-87 -0.6288849593273156 1106 88-89 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0.6619377204035075 2115 60-61 0.7173132544565348 2115 62-63 0.6558379190244139 2115 64-65 0.6314315838329705 2115 66-67 0.5953841976975696 2115 68-69 0.6375340189012348 2115 70-71 0.6295238063481108 2115 72-73 0.7237172024015734 2115 74-75 0.7570753387622418 2115 76-77 0.716255775684715 2115 78-79 0.6738628320455291 2115 80-81 0.7353572480476487 2115 82-83 0.7774990714929579 2115 84-85 0.8332959396468631 2115 86-87 0.679784461377885 2115 88-89 0.723195275074989 2115 90-91 0.9352042716221227 2115 92-93 1.016017230721392 2115 94-95 1.1108941650406159 2115 96-97 1.194514957750144 2115 98-99 1.2187280297057903 2115 100 1.2704907998030528 2116 1 0.31917004346692934 2116 2 0.2968506949846983 2116 3 0.30043749159742816 2116 4 0.11091506680499919 2116 5 0.11398235284654845 2116 6 0.17156575729840995 2116 7 0.23777028433457303 2116 8 0.1595483591956608 2116 9 0.21351648256743516 2116 10-11 0.264930775846544 2116 12-13 0.2959887863861823 2116 14-15 0.5106931936184509 2116 16-17 0.4981426321005813 2116 18-19 0.5030413479186748 2116 20-21 0.4720433282701393 2116 22-23 0.4595149805563281 2116 24-25 0.293395895001062 2116 26-27 0.33841958718480214 2116 28-29 0.4777003226908576 2116 30-31 0.5837955277949405 2116 32-33 0.5345779455083388 2116 34-35 0.5623952077618597 2116 36-37 0.712231416145201 2116 38-39 0.6997857719119622 2116 40-41 0.6310593579159374 2116 42-43 0.613916165711224 2116 44-45 0.6029879644390306 2116 46-47 0.5996072174995035 2116 48-49 0.5407161928998008 2116 50-51 0.4352555665055462 2116 52-53 0.2906272045206322 2116 54-55 0.3440922044660013 2116 56-57 0.3638465658542671 2116 58-59 0.3859694601357333 2116 60-61 0.4276829985161541 2116 62-63 0.2343868987817288 2116 64-65 0.11727928976499413 2116 66-67 0.22537430739568975 2116 68-69 0.3501164754561117 2116 70-71 0.1022052824169748 2116 72-73 0.28210995276776885 2116 74-75 0.4155237852823035 2116 76-77 0.466680920729317 2116 78-79 0.6465415744073653 2116 80-81 0.7480856559699163 2116 82-83 0.49918436149406986 2116 84-85 0.6987636774549841 2116 86-87 0.7779578841066552 2116 88-89 0.8934995409551192 2116 90-91 0.9392805146753354 2116 92-93 0.9435471220209841 2116 94-95 1.1428907227853138 2116 96-97 1.1836762024752048 2116 98-99 1.1784099784379585 2116 100 1.0599565509029958 2201 1 0.662628523726017 2201 2 0.697392302526513 2201 3 0.7243039923260959 2201 4 0.6330329470660985 2201 5 0.5660994698384627 2201 6 0.6091095998434639 2201 7 0.625239250244519 2201 8 0.6959524505750139 2201 9 0.8359092965199721 2201 10-11 0.8149296462651776 2201 12-13 0.47718055103244694 2201 14-15 0.7579291044426739 2201 16-17 0.8574455578160993 2201 18-19 0.9563364187320289 2201 20-21 0.9824139542513564 2201 22-23 0.9886998710763848 2201 24-25 0.9602203467247179 2201 26-27 0.9501665243266899 2201 28-29 0.90979634038559 2201 30-31 0.9090801484483748 2201 32-33 0.9141789495743282 2201 34-35 0.9159933912239779 2201 36-37 0.4514958676778491 2201 38-39 0.6741009266820654 2201 40-41 0.7615632609580842 2201 42-43 0.7802339984087538 2201 44-45 0.8069931570925206 2201 46-47 0.825332602941522 2201 48-49 0.7594242411563741 2201 50-51 0.9796436245285491 2201 52-53 0.9716432604686176 2201 54-55 0.9342163885750807 2201 56-57 0.8699447908293934 2201 58-59 0.8423510853175387 2201 60-61 0.7432006243684484 2201 62-63 0.7294703513268743 2201 64-65 0.7552096201793077 2201 66-67 0.6714622927335085 2201 68-69 0.015899699027634995 2201 70-71 0.478639164812364 2201 72-73 0.5153475175556821 2201 74-75 0.44318678761550956 2201 76-77 0.4439282299916627 2201 78-79 0.37579855858759714 2201 80-81 0.32968014666305123 2201 82-83 0.3754212976360911 2201 84-85 0.29554022281316605 2201 86-87 0.2556873678266811 2201 88-89 0.2187540869499962 2201 90-91 0.22069575826546028 2201 92-93 0.07928387311833518 2201 94-95 0.16265264195332563 2201 96-97 0.14030827758317344 2201 98-99 0.09272440771037793 2201 100 0.07453456460292784 2202 1 0.7787295605391797 2202 2 0.8048883555938069 2202 3 0.8351063302861483 2202 4 0.6896675689534391 2202 5 0.6828877218544918 2202 6 0.6810945542759157 2202 7 0.6966410555727052 2202 8 0.715258825250281 2202 9 0.893186460978221 2202 10-11 0.9445549033696565 2202 12-13 0.9826983498333277 2202 14-15 1.1816981730248557 2202 16-17 1.0929264058644392 2202 18-19 1.0895454338877855 2202 20-21 1.1247672312549 2202 22-23 0.674546473566032 2202 24-25 0.8482826826092733 2202 26-27 1.0056326981806265 2202 28-29 1.0900903460617073 2202 30-31 1.1257417211232408 2202 32-33 1.1597461200141979 2202 34-35 1.0245592190071235 2202 36-37 0.9596251206873134 2202 38-39 0.9746523458288294 2202 40-41 0.9299696460691109 2202 42-43 0.3047211959466054 2202 44-45 0.7306309406070497 2202 46-47 0.870229800502706 2202 48-49 0.8607345922060148 2202 50-51 1.048595358263917 2202 52-53 1.063182402439267 2202 54-55 1.0042757193688558 2202 56-57 0.8465468621820023 2202 58-59 0.8698171749274906 2202 60-61 0.8101368790732835 2202 62-63 0.8133165818603914 2202 64-65 0.7550595696912126 2202 66-67 0.7255673723834235 2202 68-69 0.79563006434919 2202 70-71 0.7466716042849555 2202 72-73 0.678132912272261 2202 74-75 0.679451483672544 2202 76-77 0.6824555256256701 2202 78-79 0.5921108393783356 2202 80-81 0.6680306611052202 2202 82-83 0.5843338812387735 2202 84-85 0.602566550078599 2202 86-87 0.49030595391528564 2202 88-89 0.5566392918406997 2202 90-91 0.42948496378346945 2202 92-93 0.4394844058667964 2202 94-95 0.4516427402243721 2202 96-97 0.4000147732899322 2202 98-99 0.42514523148245686 2202 100 0.4247766713205259 2203 1 -0.26735949270246095 2203 2 -0.12062233334217609 2203 3 0.35303518224372255 2203 4 0.37666620595491906 2203 5 0.5178584254957173 2203 6 0.5216799678489608 2203 7 0.5442401447845313 2203 8 0.6101375640351279 2203 9 0.7124446870257302 2203 10-11 0.7276768487872403 2203 12-13 0.7824165218054162 2203 14-15 0.9867902475098163 2203 16-17 0.9559333028532251 2203 18-19 0.9044284662080884 2203 20-21 0.8666254700974179 2203 22-23 0.926714115776825 2203 24-25 0.8418997467020404 2203 26-27 0.8696960842197754 2203 28-29 0.8255054659142687 2203 30-31 0.8635390473274143 2203 32-33 0.9058190518486455 2203 34-35 0.8109536148545686 2203 36-37 0.7270526096463641 2203 38-39 0.7825161835376946 2203 40-41 0.77692459233009 2203 42-43 0.7656933115437781 2203 44-45 0.6972733688468864 2203 46-47 0.7506777023019353 2203 48-49 0.900319329686134 2203 50-51 1.0381286078590435 2203 52-53 0.9186018758977426 2203 54-55 0.8745137470276916 2203 56-57 0.8342712027886705 2203 58-59 0.7884814093416068 2203 60-61 0.7661165395190537 2203 62-63 0.7368587621294083 2203 64-65 0.7117467474132511 2203 66-67 0.7996426854105465 2203 68-69 0.7487566036544919 2203 70-71 0.7670019977704428 2203 72-73 0.6921729534325394 2203 74-75 0.6556398198532136 2203 76-77 0.6018141744490109 2203 78-79 0.5350444744455416 2203 80-81 0.5529619585308296 2203 82-83 0.6056496178346507 2203 84-85 0.509601971220512 2203 86-87 0.5281881446485777 2203 88-89 0.5578020305006 2203 90-91 0.47397730690348183 2203 92-93 0.5183185375364197 2203 94-95 0.4392538104875747 2203 96-97 0.5120936944486729 2203 98-99 0.381144114472427 2203 100 0.4312708126494762 2204 1 0.7681759108345894 2204 2 0.7450042683905416 2204 3 0.797327136355225 2204 4 0.6526388038389825 2204 5 0.6929099832525338 2204 6 0.5948902020534845 2204 7 0.6979265123416525 2204 8 0.7250703180609861 2204 9 0.8704406591443643 2204 10-11 0.8822216653288706 2204 12-13 0.8283576750032751 2204 14-15 1.0319791867787416 2204 16-17 1.0573215695029177 2204 18-19 1.0932338841662457 2204 20-21 1.0720246256919381 2204 22-23 1.103155149423138 2204 24-25 1.1124382582821468 2204 26-27 1.0765629453589156 2204 28-29 1.1148230636992977 2204 30-31 1.1744069306080291 2204 32-33 1.1660630564117085 2204 34-35 1.1792847036756129 2204 36-37 1.0553789112131184 2204 38-39 1.155891823943307 2204 40-41 1.0998152125833158 2204 42-43 1.0470691712446651 2204 44-45 1.1054026355438396 2204 46-47 1.1297188537712444 2204 48-49 1.1171239906453323 2204 50-51 1.2580381218536942 2204 52-53 1.1252441559809725 2204 54-55 1.078099154188756 2204 56-57 1.0961244978150617 2204 58-59 1.084084123765983 2204 60-61 1.0461293708145973 2204 62-63 1.0018588441054845 2204 64-65 0.9999597639821332 2204 66-67 0.9296229636736868 2204 68-69 0.9276150412142741 2204 70-71 0.9073977070315067 2204 72-73 0.8284499585209133 2204 74-75 0.770044421700625 2204 76-77 0.7860348857891495 2204 78-79 0.7587300252093954 2204 80-81 0.7552981208176419 2204 82-83 0.7287879542010991 2204 84-85 0.8069776686013519 2204 86-87 0.7889953174140061 2204 88-89 0.7302897773032875 2204 90-91 0.7259984807667195 2204 92-93 0.7307828065401978 2204 94-95 0.692155837655708 2204 96-97 0.6439977768051719 2204 98-99 0.705501698405353 2204 100 0.7124519699038423 2205 1 0.7106227809141537 2205 2 0.787276137429405 2205 3 0.859023097513596 2205 4 0.6317651651084262 2205 5 0.6635044838327246 2205 6 0.7010703562850082 2205 7 0.7581144730232197 2205 8 0.7574348942025395 2205 9 0.8966382613995734 2205 10-11 0.9394603644393626 2205 12-13 0.9747195264298583 2205 14-15 1.2054995054699518 2205 16-17 1.194647746189247 2205 18-19 1.1854851559566555 2205 20-21 1.1627381601946993 2205 22-23 1.198611022381364 2205 24-25 1.243491934555017 2205 26-27 1.2959419970449133 2205 28-29 1.2879460053629614 2205 30-31 1.3142659260442926 2205 32-33 1.2959228352390397 2205 34-35 1.2425436410804807 2205 36-37 1.304210571400148 2205 38-39 1.3383749776666676 2205 40-41 1.3420926647858096 2205 42-43 1.279256703191706 2205 44-45 1.344756884580839 2205 46-47 1.3780280347320613 2205 48-49 1.3979859291335899 2205 50-51 1.4047990074111851 2205 52-53 1.3451529344441369 2205 54-55 1.2341302947664374 2205 56-57 1.2117025404453088 2205 58-59 1.1608932681794784 2205 60-61 1.208724224958722 2205 62-63 1.2572932417325688 2205 64-65 1.197984580543526 2205 66-67 1.1925771142459638 2205 68-69 1.1315893869234017 2205 70-71 1.2291929756627127 2205 72-73 0.9813580601939265 2205 74-75 1.0784447957907943 2205 76-77 1.0317955328330157 2205 78-79 1.0480370358746924 2205 80-81 1.018918117371598 2205 82-83 1.083328586757876 2205 84-85 1.0555320462295654 2205 86-87 1.024300935165563 2205 88-89 1.064325740670892 2205 90-91 0.9950866998872065 2205 92-93 1.0292162138021936 2205 94-95 0.9655036136463657 2205 96-97 0.9198939956631449 2205 98-99 0.9639844924462366 2205 100 0.9225583992791364 2206 1 0.7497585792486099 2206 2 0.7661529922235637 2206 3 0.7678951650831429 2206 4 0.6591832078741504 2206 5 0.6612469874774973 2206 6 0.6490789153552114 2206 7 0.7011811949388758 2206 8 0.6837005810094752 2206 9 0.869783762747339 2206 10-11 0.9073985459813443 2206 12-13 0.9428125394095019 2206 14-15 1.1043935004398975 2206 16-17 1.1260248769730055 2206 18-19 1.103689867195662 2206 20-21 1.0718178310385298 2206 22-23 1.139553791880914 2206 24-25 1.1792335862620646 2206 26-27 1.2009233227828986 2206 28-29 1.1618635260286183 2206 30-31 1.2251683868353496 2206 32-33 1.3015633291907207 2206 34-35 1.280806633732844 2206 36-37 1.2047846917704277 2206 38-39 1.2403966661801107 2206 40-41 1.264886957065677 2206 42-43 1.2771233930320065 2206 44-45 1.337625499599035 2206 46-47 1.368013424977697 2206 48-49 1.378548126315728 2206 50-51 1.3526693527910965 2206 52-53 1.3193005534511073 2206 54-55 1.2223963045158541 2206 56-57 1.2290729933389386 2206 58-59 1.17362866468185 2206 60-61 1.2026262595620167 2206 62-63 1.1562552034716944 2206 64-65 1.1705111574473577 2206 66-67 1.1168038891837355 2206 68-69 1.1422904865841375 2206 70-71 1.0441809908192567 2206 72-73 0.9436863200615022 2206 74-75 1.0003904155167618 2206 76-77 0.9528254982615323 2206 78-79 0.9128031758299073 2206 80-81 0.9476181308773874 2206 82-83 0.9284865874243344 2206 84-85 0.9731079462858041 2206 86-87 0.9635056086817357 2206 88-89 0.9234877231758141 2206 90-91 0.980004206178787 2206 92-93 0.9914305207961611 2206 94-95 1.038189768352158 2206 96-97 1.0137051926611989 2206 98-99 0.9631418030110304 2206 100 0.9831302891135785 2207 1 0.6460011936317933 2207 2 0.6273123212736635 2207 3 0.7403034018826382 2207 4 0.5204711906576236 2207 5 0.5042145480155042 2207 6 0.6187593011683816 2207 7 0.6793505502726518 2207 8 0.6003695334023718 2207 9 0.8418206234569254 2207 10-11 0.85058714712995 2207 12-13 0.8785874419613791 2207 14-15 1.0438231703853802 2207 16-17 1.0375990544489753 2207 18-19 1.037371588743703 2207 20-21 0.9866271818792214 2207 22-23 1.0532514090222094 2207 24-25 1.1238806984320107 2207 26-27 1.0936034695744112 2207 28-29 1.1206833380940324 2207 30-31 1.155026306093049 2207 32-33 1.1226542040056344 2207 34-35 1.1813207262146292 2207 36-37 1.2115655707344715 2207 38-39 1.1539165408961907 2207 40-41 1.185699604576655 2207 42-43 1.2608586005737763 2207 44-45 1.2286500186931235 2207 46-47 1.19886652766602 2207 48-49 1.2279691627467173 2207 50-51 1.2595653017440753 2207 52-53 1.1983255540715163 2207 54-55 1.106026281575872 2207 56-57 1.1469023327466417 2207 58-59 1.1912251647778547 2207 60-61 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18-19 1.0199331382000807 2310 20-21 1.0643665944091367 2310 22-23 1.1228385097336613 2310 24-25 1.1589345257669805 2310 26-27 1.1907168683249552 2310 28-29 1.2003725795863076 2310 30-31 1.1582190620204855 2310 32-33 1.2122282426885107 2310 34-35 1.2533336169379155 2310 36-37 1.2348609492388718 2310 38-39 1.2857794324224656 2310 40-41 1.2433650881661436 2310 42-43 1.2633681160474595 2310 44-45 1.2974860527614638 2310 46-47 1.3401500861052114 2310 48-49 1.397478908411891 2310 50-51 1.3267305738785922 2310 52-53 1.1725272446406265 2310 54-55 1.1140903711685368 2310 56-57 1.1435429824219696 2310 58-59 1.1465322027487375 2310 60-61 1.104533234055026 2310 62-63 1.1228021061170423 2310 64-65 1.1651833525115904 2310 66-67 1.1281333935101898 2310 68-69 1.1298548187397301 2310 70-71 1.1223747428695532 2310 72-73 1.0734384026217754 2310 74-75 1.1004131099114645 2310 76-77 1.0696417758430243 2310 78-79 1.0379842200947351 2310 80-81 1.1493715148697774 2310 82-83 1.159019099026981 2310 84-85 1.1270120812905589 2310 86-87 1.2050699623486025 2310 88-89 1.2285218992133267 2310 90-91 1.263023098455701 2310 92-93 1.2691495727942872 2310 94-95 1.308139001744106 2310 96-97 1.234626036548498 2310 98-99 1.3011830401244318 2310 100 1.4614260583836476 2311 1 0.6053292743284082 2311 2 0.6310703237471813 2311 3 0.6469429416982315 2311 4 0.5155323414613093 2311 5 0.5025991401878827 2311 6 0.5126657250710593 2311 7 0.5672502543529063 2311 8 0.5405993039150871 2311 9 0.7206272028547147 2311 10-11 0.6030172870965274 2311 12-13 0.7278927665936976 2311 14-15 0.9527180012132064 2311 16-17 0.9304449506473418 2311 18-19 0.9604021034790193 2311 20-21 0.9811146756595974 2311 22-23 1.037340066760585 2311 24-25 1.0692245517246306 2311 26-27 1.0690062550593353 2311 28-29 1.1031126878223532 2311 30-31 1.1301456722800154 2311 32-33 1.1557782640679406 2311 34-35 1.1237268688134705 2311 36-37 1.1202680869439732 2311 38-39 1.1352103077696114 2311 40-41 1.150445695082709 2311 42-43 1.2205739329791 2311 44-45 1.3105325130626753 2311 46-47 1.26289580914014 2311 48-49 1.2981451690854087 2311 50-51 1.3002125698100215 2311 52-53 1.1484911544410394 2311 54-55 1.0819884928023598 2311 56-57 1.0681426256662832 2311 58-59 1.0462848924533645 2311 60-61 1.0567422255075698 2311 62-63 1.059062390916914 2311 64-65 1.0996232331323341 2311 66-67 1.0884435031715327 2311 68-69 1.1046188892097746 2311 70-71 1.0624315819105448 2311 72-73 1.0690649925816444 2311 74-75 1.0716935000104115 2311 76-77 1.082456128996597 2311 78-79 1.0779652161600524 2311 80-81 1.0696704813148727 2311 82-83 1.198724969947076 2311 84-85 1.1629667482682038 2311 86-87 1.1457884838424839 2311 88-89 1.1530533874373674 2311 90-91 1.185650651569535 2311 92-93 1.2716951498444473 2311 94-95 1.3174990442438173 2311 96-97 1.3325665283185018 2311 98-99 1.4352768763709811 2311 100 1.4310280555244184 2312 1 0.6243056155356612 2312 2 0.5647445086470988 2312 3 0.6440906100218378 2312 4 0.47316990476235077 2312 5 0.5232251746321523 2312 6 0.402298634722996 2312 7 0.5104142899979607 2312 8 0.5430739466364187 2312 9 0.7008017386984804 2312 10-11 0.7130535781532714 2312 12-13 0.8126572776159406 2312 14-15 0.951701412730273 2312 16-17 0.9028141147700808 2312 18-19 0.9239489988003768 2312 20-21 0.9393870238562343 2312 22-23 0.9826417395986269 2312 24-25 1.059916259129018 2312 26-27 1.1005398539464721 2312 28-29 1.0621590669288068 2312 30-31 0.9951769554863787 2312 32-33 1.0690675491197155 2312 34-35 1.147782809296693 2312 36-37 1.1583128171115291 2312 38-39 1.2662735013208817 2312 40-41 1.3121014196848861 2312 42-43 1.3272000853761696 2312 44-45 1.3314841349307116 2312 46-47 1.3290448836393338 2312 48-49 1.2901803725076846 2312 50-51 1.2897661184917837 2312 52-53 1.1819153411411065 2312 54-55 1.1532308820184554 2312 56-57 1.0545722940852116 2312 58-59 1.1025319157740725 2312 60-61 1.1077114785749487 2312 62-63 1.1641119705215104 2312 64-65 1.1148511443289664 2312 66-67 1.072404906549167 2312 68-69 1.0845511417892268 2312 70-71 1.1144207323109896 2312 72-73 1.1197416438607348 2312 74-75 1.170286158614914 2312 76-77 1.0854559702403677 2312 78-79 1.0198145410689037 2312 80-81 0.932674330896262 2312 82-83 1.1138243674620476 2312 84-85 1.1234008899781998 2312 86-87 1.1809983866651557 2312 88-89 1.2581808193136261 2312 90-91 1.186037889900355 2312 92-93 1.3035153500144467 2312 94-95 1.3136810426589953 2312 96-97 1.3484793714916918 2312 98-99 1.2453462902176078 2312 100 1.41322740191508 2313 1 0.5669208524303109 2313 2 0.6183568900292187 2313 3 0.6032067852065985 2313 4 0.5290850849880258 2313 5 0.5006940295392113 2313 6 0.5515610074656863 2313 7 0.6006667190380597 2313 8 0.5941383223325545 2313 9 0.7493748250580552 2313 10-11 0.6539689319797048 2313 12-13 0.8038173780566709 2313 14-15 0.9472263370955005 2313 16-17 0.9503635488552931 2313 18-19 0.9781824512809081 2313 20-21 0.9982221823889219 2313 22-23 1.0610353586438421 2313 24-25 1.1539567041127654 2313 26-27 1.0823250903829233 2313 28-29 1.0727258371986892 2313 30-31 1.0928438289624225 2313 32-33 1.1540054430250848 2313 34-35 1.1405827262661248 2313 36-37 1.160694480783448 2313 38-39 1.1184858044236137 2313 40-41 1.1908829975107125 2313 42-43 1.2216299611937558 2313 44-45 1.1420494466834938 2313 46-47 1.132046988980001 2313 48-49 1.2660495624066854 2313 50-51 1.2467417760105803 2313 52-53 1.0814105182548275 2313 54-55 1.0441443272517503 2313 56-57 1.1294175372991972 2313 58-59 1.0893114227086542 2313 60-61 1.1153749199673655 2313 62-63 1.0875817847335085 2313 64-65 0.9742443768502298 2313 66-67 1.0350451771470617 2313 68-69 1.046782655355699 2313 70-71 1.0799675973756848 2313 72-73 1.0442440780276314 2313 74-75 1.0472451145900976 2313 76-77 1.0666514886200176 2313 78-79 1.0889936822304165 2313 80-81 1.12258268187788 2313 82-83 1.2062595637875866 2313 84-85 1.2683500114196065 2313 86-87 1.3888666837215737 2313 88-89 1.390713510231656 2313 90-91 1.4666655645956794 2313 92-93 1.4390044821669967 2313 94-95 1.4817934799114596 2313 96-97 1.5111943648647426 2313 98-99 1.5588115129771651 2313 100 1.730239646893704 2314 1 0.4785860834245419 2314 2 0.5276234085119071 2314 3 0.5501450147469029 2314 4 0.49186606562648905 2314 5 0.5008509918258071 2314 6 0.5280290265820753 2314 7 0.5798972213707358 2314 8 0.5678673893186996 2314 9 0.7181271999163386 2314 10-11 0.7126783950854971 2314 12-13 0.502966129419363 2314 14-15 0.8295389079352589 2314 16-17 0.877131378864604 2314 18-19 0.9425519293677169 2314 20-21 0.9529294982665419 2314 22-23 0.8088655853711586 2314 24-25 0.7553018934457114 2314 26-27 0.9385365331656104 2314 28-29 0.9513033121229242 2314 30-31 0.8769249531438206 2314 32-33 0.9598595828374528 2314 34-35 0.9765900968651167 2314 36-37 0.9561354356046508 2314 38-39 1.0694478993902408 2314 40-41 1.1061459492773196 2314 42-43 1.1566477402549609 2314 44-45 1.2035766744865768 2314 46-47 1.1820322190807175 2314 48-49 1.2089535529573894 2314 50-51 1.1076987262863724 2314 52-53 0.959460880280588 2314 54-55 0.9231498359452743 2314 56-57 0.9345045968490453 2314 58-59 0.8711203056803782 2314 60-61 0.8710158432834376 2314 62-63 0.9272927991344275 2314 64-65 0.8933551086063574 2314 66-67 0.9721742083328166 2314 68-69 0.8548009425226439 2314 70-71 0.8880057865386419 2314 72-73 0.9656491569573618 2314 74-75 0.9430253545296239 2314 76-77 0.9773994712519567 2314 78-79 1.0555267276802525 2314 80-81 1.14856020544849 2314 82-83 1.0186325269939438 2314 84-85 1.0424490334776166 2314 86-87 1.1869146840217084 2314 88-89 1.3088577440566773 2314 90-91 1.3052339127727635 2314 92-93 1.3842014610539444 2314 94-95 1.2959872520416589 2314 96-97 1.443446843607802 2314 98-99 1.4954192146088268 2314 100 1.29283719111665 2315 1 0.3415616699188462 2315 2 0.30008064733925366 2315 3 0.3508791417641106 2315 4 0.29092682738346554 2315 5 0.25391580147449844 2315 6 -0.35215940035487137 2315 7 0.13888543085879235 2315 8 0.22592436664246662 2315 9 0.3738959229029746 2315 10-11 0.5183093066176099 2315 12-13 0.5074947618081751 2315 14-15 0.609578351194699 2315 16-17 0.5190680888670727 2315 18-19 0.671055534407877 2315 20-21 0.6681388608215073 2315 22-23 0.6091565829589669 2315 24-25 0.6604781453566275 2315 26-27 0.6797773714115323 2315 28-29 0.7671862216031968 2315 30-31 0.872477689190994 2315 32-33 0.8409064484085889 2315 34-35 0.8535070811514558 2315 36-37 0.929861113217548 2315 38-39 0.9385491331992171 2315 40-41 0.8719610522724679 2315 42-43 1.016638128383704 2315 44-45 1.0033107772142174 2315 46-47 0.9597122882631695 2315 48-49 1.0778059575777448 2315 50-51 1.0412204362121855 2315 52-53 0.9088222851685828 2315 54-55 0.7499130521946924 2315 56-57 0.8181059540183355 2315 58-59 0.6689642840417989 2315 60-61 0.629218584199954 2315 62-63 0.7401685580977784 2315 64-65 0.8746090991821944 2315 66-67 0.8352404312227577 2315 68-69 0.8486170067702972 2315 70-71 0.8947859610293136 2315 72-73 0.9252555638733568 2315 74-75 1.0011710950423733 2315 76-77 0.8287411040349895 2315 78-79 0.8533662273451128 2315 80-81 0.8962505465378428 2315 82-83 1.0041834182758116 2315 84-85 0.928428251606384 2315 86-87 1.0808629253928537 2315 88-89 1.2092894707900221 2315 90-91 1.3065416267849095 2315 92-93 1.430702476099846 2315 94-95 1.4369536377240557 2315 96-97 1.4905215241075638 2315 98-99 1.5745020442440811 2315 100 1.6616393820296231 2316 1 0.030248541607672763 2316 2 -0.048913295819723146 2316 3 0.12382035188282359 2316 4 0.048751547929441585 2316 5 0.05608921467946004 2316 6 0.10673692743259977 2316 7 0.11838059450950311 2316 8 0.12906866076971113 2316 9 0.24539981765931174 2316 10-11 0.17924243434398335 2316 12-13 0.21416955816253846 2316 14-15 0.4352875298325998 2316 16-17 0.4517171095736501 2316 18-19 0.44294939213163076 2316 20-21 0.3977413897168134 2316 22-23 0.257910230573124 2316 24-25 0.36605375616573355 2316 26-27 0.4706143441702082 2316 28-29 0.6160726796813805 2316 30-31 0.6308131995485908 2316 32-33 0.6159658512120885 2316 34-35 0.6971491237163221 2316 36-37 0.7858910070762306 2316 38-39 0.8110148652394074 2316 40-41 0.7594307237279523 2316 42-43 0.8576034776670767 2316 44-45 0.7301177992319552 2316 46-47 0.716517682106435 2316 48-49 0.7620623412120793 2316 50-51 0.5466720245451313 2316 52-53 0.255597357258047 2316 54-55 0.14998747846291138 2316 56-57 0.37134403195437216 2316 58-59 0.3826908974085228 2316 60-61 0.45582127751053036 2316 62-63 0.5110040384963312 2316 64-65 0.5477810499061917 2316 66-67 0.5820380644738208 2316 68-69 0.5180649597523299 2316 70-71 0.3881577250325279 2316 72-73 0.5677365854146359 2316 74-75 0.6421282954642678 2316 76-77 0.7324037296652719 2316 78-79 0.7850737988997096 2316 80-81 0.8024831313423277 2316 82-83 0.8090302811958203 2316 84-85 0.921158236844164 2316 86-87 0.9360089713655348 2316 88-89 1.011178648510775 2316 90-91 0.9804825264479753 2316 92-93 1.131008074383665 2316 94-95 1.1222240113557476 2316 96-97 1.2227305542284697 2316 98-99 1.455313280980807 2316 100 1.2335131871628633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 20.0 1 293.0 2 131.0 3 102.0 4 71.0 5 70.0 6 86.0 7 470.0 8 1702.0 9 3214.0 10 4672.0 11 6399.0 12 8071.0 13 9414.0 14 10731.0 15 13039.0 16 16186.0 17 20215.0 18 25275.0 19 30597.0 20 36544.0 21 43514.0 22 47646.0 23 49730.0 24 49448.0 25 50425.0 26 55832.0 27 64119.0 28 76402.0 29 89738.0 30 108127.0 31 133719.0 32 169781.0 33 221361.0 34 310260.0 35 407330.0 36 585912.0 37 873172.0 38 843097.0 39 235542.0 40 277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03176983061461 18.926706210391135 12.70185741701762 23.33966654197663 2 15.933950449129286 20.458206737853143 41.65712214349683 21.95072066952074 3 17.982456235664063 28.433806679582368 30.69758900912393 22.886148075629638 4 12.243532434207353 17.159069325942365 38.62691545257423 31.970482787276055 5 12.35469203730837 38.33987404795983 33.95530610674846 15.350127807983338 6 28.327712121492088 37.02056570078921 20.65063307924659 14.00108909847211 7 26.16732462819684 33.94364075235232 23.47023672484077 16.418797894610062 8 26.20859619608139 35.277320244487626 21.098546547711152 17.415537011719834 9 28.678908339171173 15.72475516401408 20.759844137879767 34.836492358934976 10-11 24.95447354727252 25.784306991532883 29.494066237130546 19.767153224064053 12-13 25.171470499662174 24.25219017420866 28.637472603464005 21.938866722665164 14-15 23.267870385209854 25.519564727261862 27.038086364587095 24.174478522941186 16-17 22.03186594316866 27.464533245020245 27.769435045062163 22.73416576674893 18-19 21.618628987237493 27.11161776846169 29.545231808413984 21.724521435886825 20-21 22.923606161228076 26.85310582222145 28.63444437683629 21.58884363971419 22-23 22.494948965920706 26.584977223894736 28.741682391498657 22.1783914186859 24-25 21.83976274669337 26.131525465570714 29.30063994242083 22.728071845315085 26-27 21.69301551159627 26.391650801439354 29.017546025095054 22.897787661869323 28-29 21.832370323424215 27.880443421705554 28.62891068058193 21.658275574288304 30-31 23.54825503330912 26.474355227294506 28.94105751698269 21.03633222241369 32-33 23.22160160711833 26.416855444539294 28.668781326550658 21.692761621791718 34-35 22.975216507709806 27.018334522978922 28.70129276027948 21.305156209031793 36-37 22.493287889428185 27.054687089983737 28.693812253881585 21.758212766706496 38-39 22.504993603905916 27.240330695395 28.550831861664864 21.703843839034224 40-41 22.59279880249515 26.287800158554646 29.169480924763036 21.94992011418716 42-43 22.312594192304484 26.865196434522787 28.17135145643348 22.650857916739245 44-45 21.220477365568208 27.34552302283782 29.188625897944416 22.24537371364956 46-47 21.264678555541778 26.425817846962968 28.642524401269696 23.66697919622556 48-49 21.856005905095927 26.59151551087513 28.6680257139454 22.884452870083543 50-51 21.833393967765087 27.024461972648872 28.531738321786708 22.61040573779933 52-53 21.583545796585856 27.30388724339078 28.03796548688926 23.0746014731341 54-55 21.336957274223785 26.67912201631034 28.99610613644596 22.987814573019914 56-57 21.916888142797927 26.726454172753773 28.216986543626867 23.139671140821434 58-59 21.480957213330576 26.878403292397547 28.77037329120092 22.870266203070962 60-61 22.257992976023488 26.995716310974764 28.379692425847132 22.366598287154616 62-63 22.33943949264953 26.767172866781387 28.142742955844145 22.75064468472494 64-65 21.726982087598927 26.277233540999802 28.223674759841956 23.772109611559316 66-67 21.69900653810118 26.053875709790287 28.14976061591117 24.097357136197363 68-69 21.10300872922915 26.465932576523386 28.55982389834859 23.871234795898875 70-71 21.550419542944944 26.076510279071126 28.707161752228778 23.665908425755152 72-73 21.680481025521196 26.854418049868634 27.839368341543334 23.625732583066835 74-75 22.114010473170325 26.548645737711755 27.647576688503158 23.68976710061476 76-77 21.763087913729645 26.196580151842348 27.97113094025452 24.069200994173492 78-79 21.416456732934957 26.224122086578678 28.50080478520699 23.858616395279377 80-81 21.753229255402378 26.348811738321658 27.936710071104464 23.961248935171504 82-83 22.45384442624121 26.286584886846764 28.04015823122808 23.219412455683948 84-85 22.603748633521196 26.701414141238555 27.227591322938355 23.467245902301897 86-87 21.914774152024368 26.9410905247586 27.646989034084168 23.497146289132868 88-89 21.902411696932216 26.046161715946425 27.81628667949347 24.235139907627893 90-91 22.865866213362583 25.65044844182154 27.925748095704535 23.557937249111347 92-93 22.813873999051232 25.986432579368017 27.59437206511684 23.60532135646391 94-95 22.549175244973004 25.869394600135582 27.50871249941732 24.072717655474094 96-97 22.478419516365506 25.6464800420119 27.587498439385744 24.287602002236852 98-99 22.364908330652945 25.867816191875377 27.35090148044474 24.41637399702694 100 22.593711512478244 25.72737137466154 27.32336679250712 24.355550320353096 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 748.0 1 561.0 2 1154.0 3 1697.0 4 1138.0 5 908.0 6 1181.0 7 1975.5 8 3274.5 9 4000.5 10 3792.0 11 3606.0 12 3841.5 13 4295.5 14 4802.5 15 5267.5 16 5707.0 17 6236.5 18 6780.0 19 7219.0 20 7717.5 21 8353.5 22 9427.0 23 11261.0 24 13701.0 25 16772.5 26 20813.0 27 25592.0 28 31108.0 29 38758.5 30 48952.0 31 58549.0 32 69978.5 33 81380.0 34 91693.5 35 102040.5 36 112890.5 37 127344.0 38 134631.0 39 133323.0 40 130993.5 41 129225.0 42 130973.0 43 136046.0 44 142738.0 45 154397.5 46 165958.5 47 176619.0 48 188772.5 49 199206.0 50 209284.0 51 213311.0 52 206267.5 53 193211.0 54 177140.5 55 155720.5 56 136165.5 57 119247.0 58 99706.5 59 79259.0 60 59546.5 61 44509.0 62 34772.5 63 27031.5 64 19784.5 65 14097.5 66 10522.0 67 8138.5 68 6280.0 69 4864.0 70 3858.0 71 2992.5 72 2345.0 73 1850.5 74 1447.5 75 1147.5 76 855.5 77 591.0 78 428.5 79 337.5 80 249.0 81 176.0 82 131.5 83 94.5 84 65.5 85 56.0 86 44.0 87 34.5 88 33.5 89 23.5 90 19.5 91 19.0 92 13.0 93 9.0 94 6.5 95 7.5 96 7.0 97 4.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.217522889656452 2 0.14621744380622473 3 0.023877113037598957 4 0.023464314905010802 5 0.028244082756031526 6 0.056466439294558406 7 0.06346228133105238 8 0.05611881981448417 9 0.04347416122678391 10-11 0.045885771369798906 12-13 0.04378919138060118 14-15 0.022193331180989387 16-17 0.020085888083039342 18-19 0.01944496466665247 20-21 0.02289943324989017 22-23 0.0260931872230722 24-25 0.02324705272996441 26-27 0.020661632846912293 28-29 0.02019451917056254 30-31 0.01827174892140193 32-33 0.0262018183105954 34-35 0.02658202711692659 36-37 0.025278454066648217 38-39 0.023844523711342 40-41 0.02533276961040981 42-43 0.03134006875044267 44-45 0.04081269958246555 46-47 0.045679372303504835 48-49 0.04262683874410296 50-51 0.030155989896439812 52-53 0.0463746112636533 54-55 0.044180263295684696 56-57 0.04699380846253553 58-59 0.05035050906700235 60-61 0.02627786007186164 62-63 0.02737503405584594 64-65 0.027918189493461927 66-67 0.03369736334969607 68-69 0.03812951172064256 70-71 0.04244216589531352 72-73 0.04663532587370897 74-75 0.02741848649085522 76-77 0.020020709430525422 78-79 0.019227702491606075 80-81 0.02289943324989017 82-83 0.02145463978583164 84-85 0.012536027500177068 86-87 0.0142523986830436 88-89 0.019814310364231348 90-91 0.020998389218234208 92-93 0.021183062067023643 94-95 0.025104644326611098 96-97 0.02564779976422709 98-99 0.03072087155156044 100 0.030525335594018686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4602734.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7717753563512 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42612930242016 53.555410846159845 2 11.850367728896941 15.58839448310327 3 3.1832066640780985 6.2809546086775425 4 1.279206942138578 3.3654284653049413 5 0.6526660764844033 2.146350328262166 6 0.38858468393668694 1.5334742723281507 7 0.2543669148157593 1.171111450554517 8 0.18201539729225624 0.9577180657682633 9 0.12850730049380307 0.7606937969756583 >10 0.5915999648857633 6.8147359199940265 >50 0.03680916588081916 1.6739392635939945 >100 0.023036598060289515 2.949819870679361 >500 0.002422467601183227 1.075894126433479 >1k 9.689868412526613E-4 1.182203806593794 >5k 3.726872466355961E-5 0.21754667904205469 >10k+ 7.453744932711922E-5 0.7263240165290279 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17021 0.3698019481464712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12610 0.2739676027335058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8875 0.19282018035367676 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4926 0.10702334742785484 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.3452435009279266E-5 0.0 5 0.0 0.0 0.0 4.3452435009279266E-5 0.0 6 0.0 0.0 0.0 4.3452435009279266E-5 0.0 7 0.0 0.0 0.0 6.51786525139189E-5 0.0 8 0.0 0.0 0.0 6.51786525139189E-5 0.0 9 0.0 0.0 0.0 6.51786525139189E-5 0.0 10-11 0.0 0.0 0.0 6.51786525139189E-5 2.1726217504639633E-5 12-13 0.0 0.0 0.0 1.1949419627551799E-4 4.3452435009279266E-5 14-15 0.0 0.0 0.0 1.7380974003711706E-4 4.3452435009279266E-5 16-17 0.0 0.0 0.0 2.7157771880799544E-4 4.3452435009279266E-5 18-19 0.0 0.0 0.0 2.9330393631263503E-4 4.3452435009279266E-5 20-21 0.0 0.0 0.0 5.540185463683107E-4 4.3452435009279266E-5 22-23 0.0 0.0 0.0 0.001607740095343333 5.4315543761599086E-5 24-25 0.0 0.0 0.0 0.0037803618458072965 6.51786525139189E-5 26-27 0.0 0.0 0.0 0.007169651776531079 6.51786525139189E-5 28-29 0.0 0.0 0.0 0.01779377213629986 6.51786525139189E-5 30-31 0.0 0.0 0.0 0.05642298685954913 6.51786525139189E-5 32-33 0.0 0.0 0.0 0.14567428836860874 8.690487001855853E-5 34-35 0.0 0.0 0.0 0.2670152131320211 8.690487001855853E-5 36-37 0.0 0.0 0.0 0.44563731034641585 8.690487001855853E-5 38-39 0.0 0.0 0.0 0.7738118257539975 8.690487001855853E-5 40-41 0.0 0.0 0.0 1.2099439159421337 8.690487001855853E-5 42-43 0.0 0.0 0.0 1.6082506614546919 8.690487001855853E-5 44-45 0.0 0.0 0.0 2.0203970075177056 8.690487001855853E-5 46-47 0.0 0.0 0.0 2.4732365589669096 8.690487001855853E-5 48-49 0.0 0.0 0.0 2.9834115984108576 1.0863108752319817E-4 50-51 0.0 0.0 0.0 3.562219758951962 1.0863108752319817E-4 52-53 0.0 2.1726217504639633E-5 0.0 4.1779950785772115 1.1949419627551799E-4 54-55 0.0 2.1726217504639633E-5 0.0 4.822047070284748 1.412204137801576E-4 56-57 0.0 2.1726217504639633E-5 0.0 5.445491744689135 1.5208352253247743E-4 58-59 0.0 2.1726217504639633E-5 0.0 6.177730453248004 1.5208352253247743E-4 60-61 0.0 2.1726217504639633E-5 0.0 6.917182266018414 1.5208352253247743E-4 62-63 0.0 2.1726217504639633E-5 0.0 7.732154410834951 1.7380974003711706E-4 64-65 0.0 2.1726217504639633E-5 0.0 8.447370627978936 1.7380974003711706E-4 66-67 0.0 2.1726217504639633E-5 0.0 9.202649990201476 1.7380974003711706E-4 68-69 0.0 2.1726217504639633E-5 0.0 10.035796115960643 1.7380974003711706E-4 70-71 0.0 2.1726217504639633E-5 0.0 10.88869137343153 1.7380974003711706E-4 72-73 0.0 2.1726217504639633E-5 0.0 11.66903192754567 1.7380974003711706E-4 74-75 0.0 2.1726217504639633E-5 0.0 12.456531270327591 1.7380974003711706E-4 76-77 0.0 2.1726217504639633E-5 0.0 13.288677990081547 1.8467284878943688E-4 78-79 0.0 4.3452435009279266E-5 0.0 14.150035609270489 1.955359575417567E-4 80-81 0.0 4.3452435009279266E-5 0.0 14.967766983710117 1.955359575417567E-4 82-83 0.0 5.4315543761599086E-5 0.0 15.84036792045771 1.955359575417567E-4 84-85 0.0 6.51786525139189E-5 0.0 16.678424171372928 1.955359575417567E-4 86-87 0.0 6.51786525139189E-5 0.0 17.504737401726885 2.0639906629407652E-4 88 0.0 8.690487001855853E-5 0.0 18.135764526040393 2.1726217504639634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7340 0.0 53.137993 1 GTATCAA 14020 0.0 44.51832 1 TACCTGG 4025 0.0 43.496143 2 ACCTGGG 4500 0.0 38.13006 3 ATCAACG 18035 0.0 33.85947 3 CAACGCA 18305 0.0 33.35968 5 TATCAAC 18530 0.0 33.34748 2 TCAACGC 18980 0.0 32.1984 4 GTACATG 19280 0.0 31.981846 1 AACGCAG 19475 0.0 31.863123 6 TACATGG 19570 0.0 30.99815 2 ACATGGG 19545 0.0 29.680187 3 ATGGGGG 12795 0.0 29.42894 5 CATGGGG 15445 0.0 29.280218 4 CCTGGGG 4430 0.0 29.18207 4 TGGGGGG 13540 0.0 29.094925 6 ACGCAGA 21445 0.0 28.498278 7 CGCAGAG 21695 0.0 28.081068 8 TAACGCA 510 0.0 27.652742 4 GTGGTTA 3865 0.0 26.69104 1 >>END_MODULE