FastQCFastQC Report
Mon 6 Jun 2016
ERR1211376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602734
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141670.3077953233882297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114390.24852620203557277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83860.18219605999390795No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT47940.1041554867172424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76700.052.902891
TACCTGG40150.045.7649842
GTATCAA137400.040.4477841
ACCTGGG48000.037.3030853
CCTGGGG41650.032.7223174
TAACGCA5000.031.9572894
ATCAACG172400.031.8940453
CAACGCA173400.031.6785355
TATCAAC176750.031.2406882
GTACATG188150.031.2120421
TCAACGC180650.030.463514
TACATGG190800.030.4426362
AACGCAG184750.030.392356
TGGGGGG123000.029.9496486
ATGGGGG121550.029.3417825
ACATGGG193150.029.075973
ATAACGC5850.028.9206223
CATGGGG151500.028.321694
CTGGGGG32350.028.17915
ACGCAGA204500.027.1814257
Produced by FastQC (version 0.11.5)