##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211376_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4602734 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05980011010847 33.0 31.0 34.0 30.0 34.0 2 32.1958716276022 33.0 31.0 34.0 30.0 34.0 3 32.27292800322591 34.0 31.0 34.0 30.0 34.0 4 35.92409554842839 37.0 35.0 37.0 35.0 37.0 5 35.785140527347444 37.0 35.0 37.0 35.0 37.0 6 35.820705041829484 37.0 35.0 37.0 35.0 37.0 7 35.753136939914405 37.0 35.0 37.0 33.0 37.0 8 35.73105636780227 37.0 35.0 37.0 33.0 37.0 9 37.3110414375456 39.0 37.0 39.0 34.0 39.0 10-11 37.28218315462071 39.0 37.0 39.0 34.0 39.0 12-13 37.193356057508424 39.0 37.0 39.0 33.0 39.0 14-15 38.37709587388713 40.0 38.0 41.0 33.5 41.0 16-17 38.35714534013914 40.0 38.0 41.0 33.0 41.0 18-19 38.37129204077402 40.0 38.0 41.0 33.5 41.0 20-21 38.33303456163228 40.0 38.0 41.0 34.0 41.0 22-23 38.2377784594982 40.0 38.0 41.0 34.0 41.0 24-25 38.118649589570026 40.0 37.0 41.0 33.0 41.0 26-27 37.990328357015635 40.0 37.0 41.0 33.0 41.0 28-29 37.9014756229667 40.0 37.0 41.0 33.0 41.0 30-31 37.93756210982429 40.0 37.0 41.0 33.0 41.0 32-33 37.758556218977674 40.0 37.0 41.0 33.0 41.0 34-35 37.64774859898486 40.0 37.0 41.0 33.0 41.0 36-37 37.51848129828923 40.0 36.0 41.0 32.0 41.0 38-39 37.401893526760404 40.0 36.0 41.0 32.0 41.0 40-41 37.332340626245184 40.0 36.0 41.0 32.0 41.0 42-43 37.20295372272219 40.0 36.0 41.0 31.0 41.0 44-45 37.066628551639084 39.0 36.0 41.0 31.0 41.0 46-47 36.858900058095905 39.0 35.5 41.0 30.5 41.0 48-49 36.6444998776814 39.0 35.0 41.0 30.0 41.0 50-51 36.47238141504593 39.0 35.0 41.0 30.0 41.0 52-53 36.280293082328896 39.0 35.0 40.0 29.0 41.0 54-55 36.08289605699569 38.5 35.0 40.0 29.0 41.0 56-57 35.86805559478345 38.0 35.0 40.0 28.0 41.0 58-59 35.62167214094927 38.0 34.0 40.0 28.0 41.0 60-61 35.354774575285035 38.0 34.0 40.0 27.5 41.0 62-63 35.04414908617356 37.0 34.0 40.0 27.0 41.0 64-65 34.69763807771642 37.0 34.0 39.5 26.0 41.0 66-67 34.35763396277082 36.0 33.0 39.0 26.0 41.0 68-69 33.99992298055895 36.0 33.0 39.0 26.0 40.0 70-71 33.624160444640076 35.0 33.0 38.5 25.5 40.0 72-73 33.25547359460703 35.0 32.0 37.5 25.0 40.0 74-75 32.876576834550946 35.0 32.0 37.0 24.5 39.0 76-77 31.86222710241348 34.0 30.5 36.0 23.5 38.5 78-79 32.352451064954 35.0 31.5 36.0 24.5 38.5 80-81 32.25835112348443 35.0 32.0 36.0 24.5 37.5 82-83 31.97397818340143 34.5 31.5 35.0 24.0 37.0 84-85 31.685643033032107 34.0 31.0 35.0 23.5 36.5 86-87 31.428859456140632 34.0 31.0 35.0 23.0 36.0 88-89 31.214455799531322 34.0 31.0 35.0 22.5 36.0 90-91 30.996237996807984 34.0 31.0 35.0 20.5 35.5 92-93 30.80841506374255 34.0 30.0 35.0 20.0 35.0 94-95 30.63555084869123 34.0 30.0 35.0 20.0 35.0 96-97 30.461852672781003 34.0 30.0 35.0 19.5 35.0 98-99 30.277452813914515 34.0 29.0 35.0 19.0 35.0 100 28.430314678189095 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.29353997729503334 1101 2 -0.24570921090147735 1101 3 -0.30556988298788923 1101 4 -0.19447797062868233 1101 5 -0.2167485635971289 1101 6 -0.26107004215672447 1101 7 -0.2257288314569763 1101 8 -0.33745254735361385 1101 9 -0.48229304003523765 1101 10-11 -0.4557843980882268 1101 12-13 -0.4886721411631214 1101 14-15 -0.5829872892316601 1101 16-17 -0.6165484189051895 1101 18-19 -0.6291958789569279 1101 20-21 -0.7255687557453214 1101 22-23 -0.6638670712304986 1101 24-25 -0.6762407735322924 1101 26-27 -0.6724345329937549 1101 28-29 -0.6763061496596805 1101 30-31 -0.6008544816324957 1101 32-33 -0.5242640682925099 1101 34-35 -0.5602105358538054 1101 36-37 -0.6169273467859284 1101 38-39 -0.6126149864173556 1101 40-41 -0.6105576143642466 1101 42-43 -0.5774914618711549 1101 44-45 -0.6177040117455803 1101 46-47 -0.6679787683743115 1101 48-49 -0.6674447362724081 1101 50-51 -0.7813353485456531 1101 52-53 -0.8088739688635513 1101 54-55 -0.7728310902120086 1101 56-57 -0.7616342317211249 1101 58-59 -0.7661742981053195 1101 60-61 -0.9034766660422591 1101 62-63 -0.8656891668478792 1101 64-65 -0.8593878722534285 1101 66-67 -0.8258072729424342 1101 68-69 -0.8973049293741084 1101 70-71 -0.8399196189507805 1101 72-73 -0.8783635538598276 1101 74-75 -1.0839167342674294 1101 76-77 -0.9034486314519015 1101 78-79 -0.8671583008110275 1101 80-81 -0.7460888283289435 1101 82-83 -0.7903632394700146 1101 84-85 -0.7923016092364001 1101 86-87 -0.8748863158763704 1101 88-89 -0.9469017468109122 1101 90-91 -1.0936553241531008 1101 92-93 -1.097480661724326 1101 94-95 -1.0831843449645646 1101 96-97 -1.2207156404189305 1101 98-99 -1.1141287060259977 1101 100 -1.0070718929587983 1102 1 -0.3332281417174272 1102 2 -0.253505100341588 1102 3 -0.28761571336823266 1102 4 -0.1368356365866319 1102 5 -0.17658792102685084 1102 6 -0.32178348416003644 1102 7 -0.20706594461549344 1102 8 -0.310521292924129 1102 9 -0.38449006342290915 1102 10-11 -0.42542767708657436 1102 12-13 -0.4442591952618642 1102 14-15 -0.5740102044218318 1102 16-17 -0.7043112348749432 1102 18-19 -0.6301408352526963 1102 20-21 -0.6923771658563567 1102 22-23 -0.7298958923975221 1102 24-25 -0.7187638068419986 1102 26-27 -0.7487397538772953 1102 28-29 -0.6996938179800196 1102 30-31 -0.6861367873258502 1102 32-33 -0.6280911412903833 1102 34-35 -0.7483749582492649 1102 36-37 -0.7117773349739736 1102 38-39 -0.7692414924414521 1102 40-41 -0.7663572836295458 1102 42-43 -0.7938864536027879 1102 44-45 -0.7918122092414492 1102 46-47 -0.7609388439708127 1102 48-49 -0.7900528156487425 1102 50-51 -0.7943284976125042 1102 52-53 -0.7944633853530334 1102 54-55 -0.9770597696355807 1102 56-57 -0.9433020795831553 1102 58-59 -0.950086417169814 1102 60-61 -0.8900110388275166 1102 62-63 -0.9566412103158726 1102 64-65 -0.8828936601107387 1102 66-67 -0.99778931877281 1102 68-69 -0.9622706747083853 1102 70-71 -0.9653625672144202 1102 72-73 -1.0146734995248394 1102 74-75 -0.9074461460321359 1102 76-77 -0.846751253705623 1102 78-79 -0.931060970312565 1102 80-81 -0.8366865131860237 1102 82-83 -0.7950880209488709 1102 84-85 -0.760645573328059 1102 86-87 -0.7727129163960988 1102 88-89 -0.7895665235649858 1102 90-91 -0.8917890354689497 1102 92-93 -0.9012841108148031 1102 94-95 -1.0579067640526816 1102 96-97 -1.1246844568611714 1102 98-99 -1.0627467074434342 1102 100 -1.0089618055503387 1103 1 -0.3673274654729113 1103 2 -0.3016884912653133 1103 3 -0.34545432591560044 1103 4 -0.1745374842234355 1103 5 -0.1285796625816289 1103 6 -0.21426287803465982 1103 7 -0.2194497085659748 1103 8 -0.2680713489583937 1103 9 -0.44795964602907645 1103 10-11 -0.4213290619940935 1103 12-13 -0.4634457987008318 1103 14-15 -0.6808470347928974 1103 16-17 -0.7626638043151175 1103 18-19 -0.6502756211120371 1103 20-21 -0.577747319735991 1103 22-23 -0.7663189476177692 1103 24-25 -0.6366750662933995 1103 26-27 -0.7004356137183905 1103 28-29 -0.8118661162402816 1103 30-31 -0.6736294546699497 1103 32-33 -0.6680746723309383 1103 34-35 -0.5834765874096561 1103 36-37 -0.5406249453726346 1103 38-39 -0.6624746337309091 1103 40-41 -0.73949952519839 1103 42-43 -0.6875737193732618 1103 44-45 -0.7353632973102577 1103 46-47 -0.7677532595534657 1103 48-49 -0.7711660520259116 1103 50-51 -0.6489345170405016 1103 52-53 -0.820954639762995 1103 54-55 -0.7976015460243744 1103 56-57 -0.8420996622778176 1103 58-59 -0.9377423426850484 1103 60-61 -0.8756118416391416 1103 62-63 -0.8888359873448479 1103 64-65 -0.8859047458881903 1103 66-67 -0.8685409532115784 1103 68-69 -0.8561687501000463 1103 70-71 -0.8042067449080648 1103 72-73 -0.9368663685315468 1103 74-75 -0.9487472914256543 1103 76-77 -0.8656191214557687 1103 78-79 -0.7450040477025226 1103 80-81 -0.6635386977413873 1103 82-83 -0.6526624545566087 1103 84-85 -0.7373921551677114 1103 86-87 -0.7485823989978222 1103 88-89 -0.8559092005684796 1103 90-91 -0.8993592317382415 1103 92-93 -0.8771503944022179 1103 94-95 -0.9573744589150799 1103 96-97 -0.9198754545529475 1103 98-99 -1.059987259385096 1103 100 -1.0275444459310847 1104 1 -0.4237639888300784 1104 2 -0.3522817019613953 1104 3 -0.3877508563417642 1104 4 -0.1994988843427521 1104 5 -0.2633720005134066 1104 6 -0.38688590591056027 1104 7 -0.3377087638032634 1104 8 -0.34184974990962047 1104 9 -0.5304160839234768 1104 10-11 -0.5776543414775261 1104 12-13 -0.5599966178646838 1104 14-15 -0.7399003990334805 1104 16-17 -0.7938964030613889 1104 18-19 -0.7014247812852901 1104 20-21 -0.788251588231816 1104 22-23 -0.8354291704380188 1104 24-25 -0.8650724657485611 1104 26-27 -0.8885548662441991 1104 28-29 -0.8259303506304576 1104 30-31 -0.7392040450124568 1104 32-33 -0.6931259193267749 1104 34-35 -0.834411734428187 1104 36-37 -0.7996429916130339 1104 38-39 -0.8560981882190859 1104 40-41 -0.9319532350296313 1104 42-43 -0.7525171414807446 1104 44-45 -0.8869694971279856 1104 46-47 -0.9487396848752425 1104 48-49 -1.0213225351109543 1104 50-51 -0.9027563154635061 1104 52-53 -0.8277229245334965 1104 54-55 -0.8541980610559321 1104 56-57 -0.9987023092439884 1104 58-59 -1.015645927081522 1104 60-61 -0.8966625410791238 1104 62-63 -1.0482392443089665 1104 64-65 -1.0011554016705233 1104 66-67 -1.0119182155230035 1104 68-69 -1.0314327471584477 1104 70-71 -0.9618102304810705 1104 72-73 -1.130607352806372 1104 74-75 -1.0143285480879243 1104 76-77 -0.8758598301174061 1104 78-79 -0.7914229816915963 1104 80-81 -0.880654066204805 1104 82-83 -0.9623813712078579 1104 84-85 -0.8376416212683679 1104 86-87 -0.8637951080368786 1104 88-89 -0.9343902987720618 1104 90-91 -0.8494734336226877 1104 92-93 -0.9247372838078114 1104 94-95 -1.084391708867635 1104 96-97 -1.2336840099404007 1104 98-99 -1.172104286061817 1104 100 -1.1965951900651 1105 1 -0.3340676757173071 1105 2 -0.3406705503743197 1105 3 -0.4060241193425078 1105 4 -0.24337431506319263 1105 5 -0.24977232790514847 1105 6 -0.3211684395483161 1105 7 -0.35603544573412194 1105 8 -0.3612483142071454 1105 9 -0.45525338527890824 1105 10-11 -0.45200657630778807 1105 12-13 -0.5185745229327239 1105 14-15 -0.6036610237945581 1105 16-17 -0.5959429895432393 1105 18-19 -0.6227390476002697 1105 20-21 -0.7004962258013592 1105 22-23 -0.6280837009993689 1105 24-25 -0.7260448218025957 1105 26-27 -0.7237533431740104 1105 28-29 -0.7256960350428514 1105 30-31 -0.7217768871949346 1105 32-33 -0.7476265153979469 1105 34-35 -0.7692042813931792 1105 36-37 -0.918764564473328 1105 38-39 -0.8621991043941648 1105 40-41 -0.8813833774075235 1105 42-43 -0.9517413703237736 1105 44-45 -0.9706322202450011 1105 46-47 -1.046338711497171 1105 48-49 -0.8974891538748722 1105 50-51 -0.9893785066034511 1105 52-53 -1.029683512467308 1105 54-55 -1.102576518920955 1105 56-57 -1.0087330474989855 1105 58-59 -0.9418614476147695 1105 60-61 -1.071401009328227 1105 62-63 -1.008304470385859 1105 64-65 -1.0411184482766274 1105 66-67 -1.0283439705410942 1105 68-69 -1.0839219668841409 1105 70-71 -1.0239522670500207 1105 72-73 -1.0908294127653804 1105 74-75 -0.9889607552503108 1105 76-77 -0.8720001372282802 1105 78-79 -0.8519471955061775 1105 80-81 -0.7897307078693245 1105 82-83 -0.8122849314420968 1105 84-85 -0.7698598447671365 1105 86-87 -0.7866409505994874 1105 88-89 -0.901081897866387 1105 90-91 -0.8012565424168336 1105 92-93 -0.9178224162253095 1105 94-95 -0.9471707675258223 1105 96-97 -1.0271730024065526 1105 98-99 -0.9745287400513511 1105 100 -1.0506307395814858 1106 1 -0.37336161513755073 1106 2 -0.3419246145505568 1106 3 -0.38511403103457553 1106 4 -0.1752203444635967 1106 5 -0.251638556702126 1106 6 -0.31287249990455734 1106 7 -0.331068762255164 1106 8 -0.32806991327800006 1106 9 -0.5018405809123863 1106 10-11 -0.4296727739661321 1106 12-13 -0.4850640283223484 1106 14-15 -0.6310084308064532 1106 16-17 -0.6341508805627214 1106 18-19 -0.708761812023404 1106 20-21 -0.6095680811552029 1106 22-23 -0.6522863038080402 1106 24-25 -0.6901190736314575 1106 26-27 -0.7465402952060458 1106 28-29 -0.7441882713317511 1106 30-31 -0.6544494406286105 1106 32-33 -0.6097261211073572 1106 34-35 -0.643373565001987 1106 36-37 -0.6641433537889441 1106 38-39 -0.6752558642592277 1106 40-41 -0.7025624624008984 1106 42-43 -0.6295437432680089 1106 44-45 -0.7578120058041478 1106 46-47 -0.7104641127714544 1106 48-49 -0.7244866155294005 1106 50-51 -0.8347996802557915 1106 52-53 -0.7391975236221455 1106 54-55 -0.7686020628907784 1106 56-57 -0.8127169554880638 1106 58-59 -0.8039858860129314 1106 60-61 -0.8716760869417612 1106 62-63 -0.779866128800137 1106 64-65 -0.8422475346469156 1106 66-67 -0.7932166348351828 1106 68-69 -0.8914735848557029 1106 70-71 -0.818242613097155 1106 72-73 -0.7003454008915426 1106 74-75 -0.7311940706569615 1106 76-77 -0.8164571542817782 1106 78-79 -0.6105431097439826 1106 80-81 -0.640748317701334 1106 82-83 -0.5877101222573309 1106 84-85 -0.569347669891858 1106 86-87 -0.6886865212353861 1106 88-89 -0.6231100036481756 1106 90-91 -0.6570240535877936 1106 92-93 -0.5951958024621788 1106 94-95 -0.5685626207108925 1106 96-97 -0.7330476182767391 1106 98-99 -0.8581439552085222 1106 100 -0.8335942185452794 1107 1 -0.4039983306667452 1107 2 -0.3358298148433718 1107 3 -0.35201338461854803 1107 4 -0.2712747888473217 1107 5 -0.28363031294937713 1107 6 -0.3386737881229749 1107 7 -0.3698427826843158 1107 8 -0.40723736280797596 1107 9 -0.4145090393523958 1107 10-11 -0.4629123415647243 1107 12-13 -0.5073881368579762 1107 14-15 -0.6612578917491447 1107 16-17 -0.6174427719960462 1107 18-19 -0.6738265166864039 1107 20-21 -0.6661698259513571 1107 22-23 -0.6723756542476167 1107 24-25 -0.6996062450754508 1107 26-27 -0.6537172572393573 1107 28-29 -0.6900922819957245 1107 30-31 -0.7200768852237189 1107 32-33 -0.7778781530001027 1107 34-35 -0.826055998023655 1107 36-37 -0.805844253545466 1107 38-39 -0.8469355779290595 1107 40-41 -0.7938848551588649 1107 42-43 -0.7736828108365756 1107 44-45 -0.8465166976912499 1107 46-47 -0.9472795628119499 1107 48-49 -0.9790304026776724 1107 50-51 -0.9732315530383744 1107 52-53 -0.9435554609074899 1107 54-55 -0.9800580776147498 1107 56-57 -0.9494538550992786 1107 58-59 -0.8653254225341342 1107 60-61 -0.9212823215196266 1107 62-63 -0.9549408282426128 1107 64-65 -0.8686136971442195 1107 66-67 -0.807346145273165 1107 68-69 -0.8427667941942687 1107 70-71 -0.8832554628349385 1107 72-73 -0.987791922299742 1107 74-75 -0.9976279749496157 1107 76-77 -1.0562344686116631 1107 78-79 -0.77490724202476 1107 80-81 -0.735996049068568 1107 82-83 -0.7166983728938909 1107 84-85 -0.7124467015344145 1107 86-87 -0.8609108436692132 1107 88-89 -0.763611546037108 1107 90-91 -0.7890464452570285 1107 92-93 -0.831379868528618 1107 94-95 -0.6965710660408391 1107 96-97 -0.7234730248509358 1107 98-99 -0.6481476773799528 1107 100 -0.7331585423136104 1108 1 -0.4021564632165209 1108 2 -0.32681944540218666 1108 3 -0.390660523017484 1108 4 -0.1748433834453209 1108 5 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0.994167056498874 2311 34-35 0.9874629004641235 2311 36-37 1.0289437614361248 2311 38-39 1.0439068746075577 2311 40-41 1.0697826437948592 2311 42-43 1.1591478058442206 2311 44-45 1.115307693063805 2311 46-47 1.1451612966207776 2311 48-49 1.1679258385100795 2311 50-51 1.1797995422269238 2311 52-53 1.1696792395868982 2311 54-55 1.147168166515094 2311 56-57 1.2773994613606376 2311 58-59 1.207567565827489 2311 60-61 1.1717989697548887 2311 62-63 1.2487356518598176 2311 64-65 1.2204190984727106 2311 66-67 1.2664247429505693 2311 68-69 1.265422999495975 2311 70-71 1.2212703870280066 2311 72-73 1.2608477857895863 2311 74-75 1.1293407982708672 2311 76-77 1.0801974741567477 2311 78-79 1.0060169805405792 2311 80-81 0.9862114426179289 2311 82-83 1.0672200891363666 2311 84-85 1.1399847525101698 2311 86-87 1.1626007175976447 2311 88-89 1.2010555458588748 2311 90-91 1.2145246113473327 2311 92-93 1.225109965952246 2311 94-95 1.286144846904378 2311 96-97 1.2964674161756484 2311 98-99 1.393684939377632 2311 100 1.4885522074256414 2312 1 0.11296433686366925 2312 2 0.1388496995776478 2312 3 0.339121310806604 2312 4 0.25054299812057934 2312 5 0.30937936351549666 2312 6 0.4145784200131928 2312 7 0.46306105655971663 2312 8 0.2836158396759032 2312 9 0.33772840198462006 2312 10-11 0.2520897196803418 2312 12-13 0.49972156873160145 2312 14-15 0.7819722309432748 2312 16-17 0.8384680469494654 2312 18-19 0.8296395504588858 2312 20-21 0.9003257807220066 2312 22-23 0.5280022234924076 2312 24-25 0.46433047861140153 2312 26-27 0.7750156567063087 2312 28-29 0.9479706598431363 2312 30-31 0.8801395433591921 2312 32-33 0.6632256903518083 2312 34-35 0.8772204491856499 2312 36-37 1.0072360758859276 2312 38-39 1.0171766803043383 2312 40-41 0.9661284902964411 2312 42-43 0.9207772482326888 2312 44-45 0.8392541843282757 2312 46-47 0.9831461760328963 2312 48-49 1.0733946820000924 2312 50-51 1.0856492635120176 2312 52-53 1.1415336117780228 2312 54-55 1.10987891712346 2312 56-57 1.0612686798308104 2312 58-59 1.201874389156501 2312 60-61 1.1153127491440813 2312 62-63 1.1798321567882866 2312 64-65 1.2268682702486586 2312 66-67 1.2034074350927497 2312 68-69 1.2027214725216453 2312 70-71 1.0093116883353233 2312 72-73 1.0925494331169077 2312 74-75 1.1800915472196607 2312 76-77 1.105854503783121 2312 78-79 0.980372071935129 2312 80-81 0.9202784891794522 2312 82-83 0.8925368729614682 2312 84-85 0.8515649050327347 2312 86-87 0.9817752297822615 2312 88-89 0.6106153796414482 2312 90-91 1.0427215177610485 2312 92-93 1.1720917631619052 2312 94-95 1.2786602622027878 2312 96-97 1.3195345933767477 2312 98-99 1.2780895057965402 2312 100 1.4454016129127076 2313 1 -0.3237667818203036 2313 2 0.03417094592145986 2313 3 0.27460771543065476 2313 4 0.1134790735833704 2313 5 0.21782374437024998 2313 6 0.304854923045518 2313 7 0.13875781657395692 2313 8 0.10906186672607987 2313 9 0.2857740262945754 2313 10-11 0.4328899866938727 2313 12-13 0.6080505575339146 2313 14-15 0.664832732885678 2313 16-17 0.7799895483002643 2313 18-19 0.6458136218690953 2313 20-21 0.8693352195272652 2313 22-23 0.9025400241609702 2313 24-25 0.7283367435345482 2313 26-27 0.8817766719452038 2313 28-29 0.7917012799240695 2313 30-31 0.7899521892235981 2313 32-33 0.9058798402119663 2313 34-35 0.9475016167654786 2313 36-37 0.7958065098424711 2313 38-39 0.5890977490136251 2313 40-41 0.6944895109768581 2313 42-43 0.7176686914478765 2313 44-45 0.8917761803566364 2313 46-47 0.9258313946761234 2313 48-49 1.0022481670877923 2313 50-51 0.8813694768873148 2313 52-53 0.823568017756493 2313 54-55 0.9191323756532839 2313 56-57 1.062879495219029 2313 58-59 0.8908575293079366 2313 60-61 0.93806894579442 2313 62-63 1.0334102376383285 2313 64-65 1.0917603152798137 2313 66-67 1.148599374251127 2313 68-69 1.1533834091711128 2313 70-71 1.0916479227527915 2313 72-73 1.090950461835078 2313 74-75 0.6906194519004529 2313 76-77 0.9122869499748418 2313 78-79 0.8381054206274499 2313 80-81 0.8971991142423477 2313 82-83 0.9519075881444827 2313 84-85 1.0249403555571597 2313 86-87 1.0304600399053392 2313 88-89 0.973043342940425 2313 90-91 1.0900584347374505 2313 92-93 0.8242951628806665 2313 94-95 0.8503705734990668 2313 96-97 1.1321770399061855 2313 98-99 1.3348692211874003 2313 100 1.3805324236228387 2314 1 -0.31494007259264123 2314 2 -0.17186943083708073 2314 3 0.09025590753233814 2314 4 -0.16662771645070507 2314 5 0.18082114471552302 2314 6 0.21588561013367524 2314 7 0.1257395912175383 2314 8 0.1888887561488346 2314 9 0.2688092401008433 2314 10-11 0.44786220360687423 2314 12-13 0.5393870804672929 2314 14-15 0.5815365825349019 2314 16-17 0.7038164068343278 2314 18-19 0.8017351716144887 2314 20-21 0.4747257174029542 2314 22-23 0.6589431889528399 2314 24-25 0.8365708737309987 2314 26-27 0.8327835091176823 2314 28-29 0.7827737847349283 2314 30-31 0.7102772078752935 2314 32-33 0.855590727677594 2314 34-35 0.9038027163002127 2314 36-37 0.865118699180357 2314 38-39 0.872495096291324 2314 40-41 0.8331708116811711 2314 42-43 0.6757467359735259 2314 44-45 0.6875342911325717 2314 46-47 0.8232164215411686 2314 48-49 0.7364779232199012 2314 50-51 0.7987233313604065 2314 52-53 0.9734564953227292 2314 54-55 1.029230747855614 2314 56-57 0.8823914916483986 2314 58-59 0.8278719210195931 2314 60-61 0.8829824413793403 2314 62-63 0.7002194220399431 2314 64-65 0.8490996858723108 2314 66-67 0.922434483579373 2314 68-69 0.8747087342903441 2314 70-71 0.9426090333908874 2314 72-73 0.9594609735854931 2314 74-75 0.9802724058322596 2314 76-77 0.8285496083320112 2314 78-79 0.769344852974136 2314 80-81 0.8282182989379478 2314 82-83 0.843970861047449 2314 84-85 0.7634709479409771 2314 86-87 0.8812315927313144 2314 88-89 1.0036371684563612 2314 90-91 1.0673537206688124 2314 92-93 1.0974745668965902 2314 94-95 1.1489401850634735 2314 96-97 1.2697520978564576 2314 98-99 1.275472169804825 2314 100 1.3275078720499423 2315 1 -0.024026991572071665 2315 2 0.12214231975889334 2315 3 0.11755460916474902 2315 4 -0.11646906107701938 2315 5 0.21101756302015673 2315 6 0.3600427238550097 2315 7 0.42744567711560677 2315 8 0.30757114192891066 2315 9 0.5895802545782445 2315 10-11 0.5446930942689079 2315 12-13 0.613703857759397 2315 14-15 0.7616243336038409 2315 16-17 0.6958105405918076 2315 18-19 0.7670978968353026 2315 20-21 0.7798990172970974 2315 22-23 0.846212758789072 2315 24-25 0.8408394638441834 2315 26-27 0.8489279025561984 2315 28-29 0.8789524039148588 2315 30-31 0.8635236237656798 2315 32-33 0.9166816851990589 2315 34-35 0.8916713984326421 2315 36-37 0.9732208998974272 2315 38-39 0.9590614846151766 2315 40-41 0.9620911054954391 2315 42-43 0.990950272953846 2315 44-45 0.8900946704186765 2315 46-47 0.9204917492812257 2315 48-49 1.0208492304405965 2315 50-51 0.91540254655024 2315 52-53 0.9383542206908189 2315 54-55 0.8688048342742505 2315 56-57 0.8941141529735788 2315 58-59 0.9523033676545936 2315 60-61 1.0348694420998896 2315 62-63 1.0042783601174605 2315 64-65 1.0496737305442707 2315 66-67 1.044366915609352 2315 68-69 0.9475624816043151 2315 70-71 0.5763488623772517 2315 72-73 0.8473842075496307 2315 74-75 1.0384057890654645 2315 76-77 1.0379782322439937 2315 78-79 0.9393066143859983 2315 80-81 0.8420181274202108 2315 82-83 0.8754080417543797 2315 84-85 0.7897167635879576 2315 86-87 0.7368603515468699 2315 88-89 0.9250187829231642 2315 90-91 1.045294570948375 2315 92-93 1.0795279067341674 2315 94-95 1.0748045661434062 2315 96-97 1.1375709369998894 2315 98-99 1.1563500544817877 2315 100 1.094687865993567 2316 1 0.5026800710781316 2316 2 0.4859342438536842 2316 3 0.46626203196267113 2316 4 0.29884354442876315 2316 5 0.41739204986158285 2316 6 0.400326828318299 2316 7 0.48686557724251855 2316 8 0.44406626547444006 2316 9 0.4540746904237736 2316 10-11 0.41962241887369345 2316 12-13 0.5710702128359699 2316 14-15 0.7616353425559268 2316 16-17 0.7556780848429199 2316 18-19 0.7665933544841579 2316 20-21 0.8128640540476653 2316 22-23 0.813795101836007 2316 24-25 0.9415746029971714 2316 26-27 0.9505130478527946 2316 28-29 0.8759471517339605 2316 30-31 0.8085315433405782 2316 32-33 0.7837333935599347 2316 34-35 0.7526295319790606 2316 36-37 0.6868118267313505 2316 38-39 0.6590675563158896 2316 40-41 0.7589548164262325 2316 42-43 0.9038268322424869 2316 44-45 0.9074576413210096 2316 46-47 0.8327084744838871 2316 48-49 0.6895439889490902 2316 50-51 0.69124705221234 2316 52-53 0.837067323724547 2316 54-55 0.9944577218552766 2316 56-57 1.0199050439205664 2316 58-59 0.9083586028746851 2316 60-61 1.0970995170550353 2316 62-63 1.0726936765896724 2316 64-65 0.9851825382651711 2316 66-67 1.0577074966359632 2316 68-69 1.0145796451837654 2316 70-71 0.8584265600139034 2316 72-73 0.8991588777883734 2316 74-75 0.9214794213789261 2316 76-77 0.8668880440004969 2316 78-79 0.7825901709581942 2316 80-81 0.78387361998454 2316 82-83 0.6863141264453425 2316 84-85 0.8315537534432629 2316 86-87 0.8522583123821654 2316 88-89 0.7731891957492785 2316 90-91 0.8362487914434631 2316 92-93 0.9432737817724934 2316 94-95 0.9120975750753608 2316 96-97 1.0060025352169184 2316 98-99 1.0945975986785754 2316 100 0.725193496457397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 5.0 12 25.0 13 124.0 14 576.0 15 1550.0 16 3833.0 17 7791.0 18 12916.0 19 17937.0 20 23649.0 21 31137.0 22 37235.0 23 39000.0 24 40315.0 25 43757.0 26 51118.0 27 62457.0 28 74873.0 29 91438.0 30 111969.0 31 138634.0 32 173403.0 33 226383.0 34 320618.0 35 424129.0 36 632640.0 37 990301.0 38 907216.0 39 137694.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7679823075062 19.81395405455519 13.491287329342494 23.926776308596114 2 15.612416446399033 20.925476032288636 41.0761082434918 22.38599927782053 3 17.500055401854635 28.13195374749008 31.436033453160668 22.93195739749462 4 12.120646483733712 17.654898226639585 38.495430515080486 31.729024774546215 5 12.16038524580191 38.454076072314415 34.088252471229104 15.297286210654573 6 28.396122826128995 37.415218867742524 20.240252858409807 13.948405447718681 7 26.0124591302247 33.95135798783721 23.366050879815 16.67013200212309 8 26.132587454399175 35.64645460188372 20.954945485412622 17.266012458304484 9 28.335607390541078 15.62629273634262 20.583491213738203 35.454608659378096 10-11 24.847084286849956 25.891634451872697 29.507578053659383 19.753703207617963 12-13 24.964822434905617 24.191366823164692 28.68629542028459 22.157515321645104 14-15 23.091630895343148 25.622475344808766 26.887419592600697 24.398474167247393 16-17 22.01877298295588 27.59000823749984 27.656327552454453 22.734891227089825 18-19 21.659974267467987 27.182224738601015 29.39232421426048 21.765476779670518 20-21 22.75934754416561 26.92273264601705 28.442340670206157 21.875579139611183 22-23 22.32110306613417 26.747548304985692 28.576537336287522 22.35481129259262 24-25 21.682810350120548 26.25680330082436 29.16555591259012 22.894830436464975 26-27 21.719973576571398 26.493658605261416 28.853701827403217 22.93266599076397 28-29 21.80276336259485 27.941250436293824 28.5510026257474 21.704983575363926 30-31 23.437503903965755 26.474900609784513 28.87904826990903 21.2085472163407 32-33 23.091890238132308 26.51616595067519 28.585687507720614 21.806256303471887 34-35 22.904490060238345 27.060023208713265 28.67695122693254 21.358535504115853 36-37 22.375787714367025 27.072457714463738 28.718197083409898 21.833557487759343 38-39 22.432472251914394 27.197306995514193 28.581446283874158 21.788774468697255 40-41 22.556749787327142 26.324940228362472 29.19888896287885 21.919421021431535 42-43 22.317784039610977 26.846379275825832 28.206874128425856 22.62896255613733 44-45 21.077721526716033 27.40832893062759 29.18383884869326 22.330110693963114 46-47 21.211737987903568 26.42904039144734 28.593041373172966 23.766180247476125 48-49 21.741477939672325 26.63079688641877 28.61674729165125 23.010977882257645 50-51 21.701772700719633 26.958242250592996 28.52761945921704 22.81236558947033 52-53 21.438404840133842 27.288084160406466 28.049260526053605 23.224250473406087 54-55 21.209742613248032 26.687444789551435 28.92073086004285 23.182081737157688 56-57 21.79798931388465 26.670281284002463 28.152582188185484 23.37914721392741 58-59 21.10343138804612 26.89447925795776 28.82747935062721 23.174610003368908 60-61 22.094835905972758 27.13996627172165 28.175550865520115 22.58964695678548 62-63 22.12976782379085 27.05078193955947 27.8249162799272 22.994533956722478 64-65 21.36647524352722 26.52678370228302 27.921973503247717 24.184767550942045 66-67 21.20216220351918 26.32014809994268 27.954353239751455 24.523336456786684 68-69 20.6949622637695 26.682228962478106 28.279728300009932 24.343080473742457 70-71 21.122200771802127 26.32857746979887 28.41696660483685 24.13225515356215 72-73 21.114294544852292 26.92689390036997 27.590991940276062 24.36781961450168 74-75 21.41847915791267 26.73887619186084 27.319549261480653 24.523095388745837 76-77 20.95452448286331 26.40928074084586 27.706069595994126 24.930125180296702 78-79 20.668419317752427 26.28882108031188 28.17164985829514 24.871109743640556 80-81 20.787650730184755 26.51933299533865 27.66903273808392 25.023983536392674 82-83 21.393520715871535 26.397762814528146 27.71408472375071 24.49463174584961 84-85 21.435940095131876 26.796829777933606 26.977351240424806 24.78987888650971 86-87 20.63300955046258 26.891352959556574 27.338874322433398 25.13676316754745 88-89 20.634121124269463 26.194773258296237 27.556077821999654 25.615027795434642 90-91 21.407091650463368 25.713723124643366 27.717691027165582 25.161494197727684 92-93 21.0943482210876 26.11620669500722 27.408623668609604 25.380821415295575 94-95 20.46055024502508 25.846734715428042 27.52888393107401 26.16383110847287 96-97 20.367933127875904 25.638286650593784 27.759783625494027 26.233996596036285 98-99 20.207207609520832 25.806422171162062 27.648032172028035 26.338338047289067 100 20.11424404208433 25.88991988749002 27.7813765305353 26.214459539890345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 654.0 1 512.0 2 920.5 3 1338.0 4 946.5 5 735.0 6 998.5 7 1787.0 8 2785.0 9 3314.0 10 3083.5 11 2885.0 12 3245.5 13 3646.0 14 4119.5 15 4814.5 16 5438.0 17 5927.5 18 6463.0 19 6928.5 20 7541.0 21 8370.5 22 9439.5 23 11177.0 24 13479.5 25 16482.0 26 20743.5 27 25643.5 28 31360.0 29 39272.0 30 49299.5 31 58917.5 32 70160.5 33 81493.0 34 91759.0 35 102043.0 36 112544.0 37 126831.0 38 134185.0 39 132751.0 40 130780.0 41 129393.0 42 131476.0 43 137173.0 44 143619.0 45 155056.0 46 167646.0 47 177440.5 48 188771.5 49 199103.0 50 208611.5 51 214540.5 52 211184.0 53 199970.5 54 182592.5 55 158516.5 56 137314.5 57 118863.0 58 98177.5 59 77904.0 60 58129.0 61 42932.5 62 33182.0 63 25451.5 64 18313.5 65 12903.0 66 9484.5 67 7185.5 68 5562.5 69 4256.0 70 3308.5 71 2536.0 72 1990.5 73 1594.5 74 1233.5 75 916.0 76 643.5 77 416.5 78 274.5 79 188.0 80 135.5 81 96.0 82 54.5 83 30.0 84 19.5 85 15.5 86 12.0 87 10.0 88 6.5 89 4.5 90 4.5 91 2.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05138250439847273 2 0.0 3 0.0 4 0.01722889048117923 5 0.0036065521057701794 6 0.0 7 1.0863108752319816E-4 8 4.562505675974323E-4 9 0.0020422644454361257 10-11 7.604176126623872E-4 12-13 2.9330393631263503E-4 14-15 3.2589326256959446E-5 16-17 5.431554376159908E-5 18-19 0.0 20-21 2.1726217504639633E-5 22-23 0.0 24-25 1.0863108752319816E-4 26-27 1.0863108752319817E-5 28-29 9.668166789564637E-4 30-31 9.233642439471844E-4 32-33 0.013198677134068578 34-35 0.022030384549704587 36-37 0.0297106024375947 38-39 0.019227702491606075 40-41 1.6294663128479725E-4 42-43 0.0015316983340770943 44-45 0.009787660985840156 46-47 0.01376355878918921 48-49 0.005485869919921508 50-51 0.010048375595895831 52-53 0.015479929972055738 54-55 0.009939744508372633 56-57 0.0027375034055845938 58-59 0.007701944105394751 60-61 0.001477382790315495 62-63 9.668166789564637E-4 64-65 0.019271154926615356 66-67 0.044690829407043726 68-69 0.054597984589159404 70-71 0.04785199405396879 72-73 0.03980243046849981 74-75 0.027451075817112175 76-77 0.028048546798489764 78-79 0.012231860455112114 80-81 0.0021183062067023643 82-83 0.026897057270743867 84-85 0.047211070637581926 86-87 0.04717848131132496 88-89 0.05861733482751773 90-91 0.03394721485099943 92-93 0.02608232411431988 94-95 0.007560723691614593 96-97 0.0034436054744853817 98-99 0.021128746523262045 100 0.018054486746355534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4602734.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.09173534753133 #Duplication Level Percentage of deduplicated Percentage of total 1 80.9376533181566 52.68372309435689 2 12.009900470668427 15.63490525973882 3 3.3862712273435935 6.612548116356279 4 1.3475499443259698 3.5085745737458636 5 0.6728606031726955 2.1898832153748695 6 0.3964913237443123 1.5484984987654282 7 0.2609344526100509 1.188927343264247 8 0.17461304791269683 0.9092693042367256 9 0.11980210888111835 0.7018314448829299 >10 0.6282727316797612 7.053764603604032 >50 0.037454880375781784 1.6822001902674244 >100 0.024773706851555503 3.139214800685408 >500 0.0022563853963384435 0.9850707363333662 >1k 0.0010529796344025827 1.3295099200848697 >5k 3.7606415514375E-5 0.20527811370815913 >10k+ 7.521283102875E-5 0.6268007845946962 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14167 0.3077953233882297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11439 0.24852620203557277 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8386 0.18219605999390795 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 4794 0.1041554867172424 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.1726217504639633E-5 0.0 8 0.0 0.0 0.0 2.1726217504639633E-5 0.0 9 0.0 0.0 0.0 2.1726217504639633E-5 0.0 10-11 0.0 0.0 0.0 2.1726217504639633E-5 0.0 12-13 0.0 0.0 0.0 7.604176126623871E-5 2.1726217504639633E-5 14-15 0.0 1.0863108752319817E-5 0.0 1.3035730502783779E-4 3.2589326256959446E-5 16-17 0.0 2.1726217504639633E-5 0.0 2.2812528379871616E-4 4.3452435009279266E-5 18-19 0.0 2.1726217504639633E-5 0.0 2.498515013033558E-4 4.3452435009279266E-5 20-21 0.0 2.1726217504639633E-5 0.0 5.105661113590314E-4 4.3452435009279266E-5 22-23 0.0 2.1726217504639633E-5 0.0 0.0015642876603340536 5.4315543761599086E-5 24-25 0.0 2.1726217504639633E-5 0.0 0.0037151831932933773 6.51786525139189E-5 26-27 0.0 2.1726217504639633E-5 0.0 0.00710447312401716 6.51786525139189E-5 28-29 0.0 2.1726217504639633E-5 0.0 0.01775031970129058 6.51786525139189E-5 30-31 0.0 2.1726217504639633E-5 0.0 0.05639039753329217 7.604176126623871E-5 32-33 0.0 2.1726217504639633E-5 0.0 0.14540271064980076 1.303573050278378E-4 34-35 0.0 2.1726217504639633E-5 0.0 0.26699348691451646 1.303573050278378E-4 36-37 0.0 2.1726217504639633E-5 0.0 0.44587629873896684 1.303573050278378E-4 38-39 0.0 2.1726217504639633E-5 0.0 0.7745070647141459 1.303573050278378E-4 40-41 0.0 2.1726217504639633E-5 0.0 1.2116494240162479 1.303573050278378E-4 42-43 0.0 2.1726217504639633E-5 0.0 1.6116942669291774 1.303573050278378E-4 44-45 0.0 2.1726217504639633E-5 0.0 2.0259589191988936 1.303573050278378E-4 46-47 0.0 2.1726217504639633E-5 0.0 2.4797870135445583 1.303573050278378E-4 48-49 0.0 2.1726217504639633E-5 0.0 2.9898208325747264 1.303573050278378E-4 50-51 0.0 2.1726217504639633E-5 0.0 3.568476909593298 1.303573050278378E-4 52-53 0.0 2.1726217504639633E-5 0.0 4.1834592222796285 1.8467284878943688E-4 54-55 0.0 2.1726217504639633E-5 0.0 4.826815975027016 1.955359575417567E-4 56-57 0.0 2.1726217504639633E-5 0.0 5.448891897728611 1.955359575417567E-4 58-59 0.0 2.1726217504639633E-5 0.0 6.179631497279661 1.955359575417567E-4 60-61 0.0 2.1726217504639633E-5 0.0 6.917942683631077 1.955359575417567E-4 62-63 0.0 2.1726217504639633E-5 0.0 7.729688485148175 2.3898839255103598E-4 64-65 0.0 2.1726217504639633E-5 0.0 8.441710948318978 2.498515013033558E-4 66-67 0.0 2.1726217504639633E-5 0.0 9.194828551899807 2.7157771880799544E-4 68-69 0.0 2.1726217504639633E-5 0.0 10.025063364513352 2.8244082756031526E-4 70-71 0.0 2.1726217504639633E-5 0.0 10.875101624382378 2.8244082756031526E-4 72-73 0.0 2.1726217504639633E-5 0.0 11.654171194772498 2.8244082756031526E-4 74-75 0.0 2.1726217504639633E-5 0.0 12.440138839220342 2.8244082756031526E-4 76-77 0.0 2.1726217504639633E-5 0.0 13.271698951101673 2.8244082756031526E-4 78-79 0.0 2.1726217504639633E-5 0.0 14.1315791875003 2.8244082756031526E-4 80-81 0.0 2.1726217504639633E-5 0.0 14.946768594491882 2.9330393631263503E-4 82-83 0.0 2.1726217504639633E-5 0.0 15.81699051042272 3.0416704506495485E-4 84-85 0.0 2.1726217504639633E-5 0.0 16.654481879682812 3.0416704506495485E-4 86-87 0.0 2.1726217504639633E-5 0.0 17.479317727246457 3.258932625695945E-4 88 0.0 2.1726217504639633E-5 0.0 18.109215088249723 3.4761948007423413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7670 0.0 52.90289 1 TACCTGG 4015 0.0 45.764984 2 GTATCAA 13740 0.0 40.447784 1 ACCTGGG 4800 0.0 37.303085 3 CCTGGGG 4165 0.0 32.722317 4 TAACGCA 500 0.0 31.957289 4 ATCAACG 17240 0.0 31.894045 3 CAACGCA 17340 0.0 31.678535 5 TATCAAC 17675 0.0 31.240688 2 GTACATG 18815 0.0 31.212042 1 TCAACGC 18065 0.0 30.46351 4 TACATGG 19080 0.0 30.442636 2 AACGCAG 18475 0.0 30.39235 6 TGGGGGG 12300 0.0 29.949648 6 ATGGGGG 12155 0.0 29.341782 5 ACATGGG 19315 0.0 29.07597 3 ATAACGC 585 0.0 28.920622 3 CATGGGG 15150 0.0 28.32169 4 CTGGGGG 3235 0.0 28.1791 5 ACGCAGA 20450 0.0 27.181425 7 >>END_MODULE