FastQCFastQC Report
Mon 6 Jun 2016
ERR1211375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14851622
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355410.23930719486396837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251640.16943603870338203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182140.12263980324842634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189450.049.3917731
GTATCAA354300.043.8048741
CAACGCA430850.035.0893755
ATCAACG431450.035.0624853
TATCAAC456300.033.732352
TCAACGC458700.033.1022834
AACGCAG467650.032.8307576
TACCTGG104250.032.3285522
ACGCAGA510250.029.5374837
CGCAGAG523350.028.7148698
GTACATG470000.027.0466861
TACATGG470550.026.3487072
ACATGGG473700.025.4151883
GCAGAGT590100.024.6694979
ACCTGGG136700.023.7627493
CATGGGG363500.023.355954
ATGGGGG271350.022.1924345
AGAGTAC544100.021.43769510-11
GTACTAG62400.020.6133231
TAACGCA15300.020.585794
Produced by FastQC (version 0.11.5)