##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211375_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14851622 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.343866010056004 31.0 31.0 34.0 28.0 34.0 2 31.410072044656133 31.0 31.0 34.0 28.0 34.0 3 31.481952947630905 31.0 31.0 34.0 28.0 34.0 4 35.10618314955767 37.0 35.0 37.0 32.0 37.0 5 35.15673742571687 37.0 35.0 37.0 32.0 37.0 6 35.208668588521846 37.0 35.0 37.0 32.0 37.0 7 35.13204194127753 37.0 35.0 37.0 32.0 37.0 8 35.13278078313601 37.0 35.0 37.0 32.0 37.0 9 36.70827603880573 39.0 37.0 39.0 32.0 39.0 10-11 36.63249801940825 39.0 37.0 39.0 32.0 39.0 12-13 36.54175523723941 39.0 37.0 39.0 32.0 39.0 14-15 37.73532493622582 40.0 37.0 41.0 32.0 41.0 16-17 37.735429335597146 40.0 37.0 41.0 32.0 41.0 18-19 37.72861832869164 40.0 37.0 41.0 32.0 41.0 20-21 37.709560544969435 40.0 37.0 41.0 32.0 41.0 22-23 37.58579588141955 40.0 37.0 41.0 32.0 41.0 24-25 37.49612170307054 40.0 37.0 41.0 31.5 41.0 26-27 37.36427293934629 40.0 37.0 41.0 31.0 41.0 28-29 37.24742125809558 40.0 36.0 41.0 31.0 41.0 30-31 37.09952744555443 39.5 36.0 41.0 30.5 41.0 32-33 36.92512090598589 39.0 36.0 41.0 30.0 41.0 34-35 36.80794417606373 39.0 36.0 41.0 30.0 41.0 36-37 36.682153875179424 39.0 35.5 41.0 30.0 41.0 38-39 36.53440533296633 39.0 35.0 41.0 30.0 41.0 40-41 36.38001728026744 39.0 35.0 40.5 29.0 41.0 42-43 36.19361366724793 39.0 35.0 40.0 28.5 41.0 44-45 36.0671718887001 39.0 35.0 40.0 28.0 41.0 46-47 35.86300445836825 38.5 35.0 40.0 27.0 41.0 48-49 35.64269852141402 38.0 34.0 40.0 27.0 41.0 50-51 34.99725198365539 37.5 33.5 39.5 26.5 40.5 52-53 35.3071147043737 38.0 34.0 40.0 26.5 40.5 54-55 35.7211599514181 39.0 35.0 40.0 27.0 41.0 56-57 35.65014962675457 38.5 34.0 40.0 27.0 41.0 58-59 35.45336041410157 38.0 34.0 40.0 26.5 41.0 60-61 35.23885906199337 38.0 34.0 40.0 26.0 41.0 62-63 34.97902424395127 37.5 34.0 40.0 26.0 41.0 64-65 34.77890542864611 37.0 34.0 40.0 26.0 41.0 66-67 34.58914541455472 36.5 33.5 40.0 26.0 41.0 68-69 34.26984648545458 36.0 33.0 39.0 26.0 41.0 70-71 33.91793074857413 36.0 33.0 39.0 25.0 41.0 72-73 33.544064479960504 35.0 32.5 39.0 24.5 40.5 74-75 33.170158047383644 35.0 32.0 37.5 24.0 40.0 76-77 32.800521350462596 35.0 32.0 37.0 23.0 39.0 78-79 32.43013867441549 35.0 31.5 37.0 22.5 39.0 80-81 32.059662607895625 35.0 31.0 36.0 21.5 39.0 82-83 31.716558871482185 35.0 31.0 36.0 20.5 37.0 84-85 31.402463515432856 35.0 31.0 35.0 20.0 37.0 86-87 31.099340799274316 34.0 30.5 35.0 19.5 36.5 88-89 30.828390191993844 34.0 30.0 35.0 19.0 36.0 90-91 30.573135143084034 34.0 30.0 35.0 18.0 36.0 92-93 30.32955447559869 34.0 29.0 35.0 18.0 36.0 94-95 30.091649753811403 34.0 29.0 35.0 17.0 35.0 96-97 29.852804899020455 34.0 29.0 35.0 15.0 35.0 98-99 29.597080507435486 34.0 29.0 35.0 14.0 35.0 100 28.389367302776762 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3331653714049949 1101 2 -0.3040118284202329 1101 3 -0.41300759345267934 1101 4 -0.2653264897159886 1101 5 -0.23460099035416704 1101 6 -0.2809897341964458 1101 7 -0.32247785451087907 1101 8 -0.3128750139130645 1101 9 -0.491292175480595 1101 10-11 -0.43067795392476427 1101 12-13 -0.4345090009887329 1101 14-15 -0.6768073004923494 1101 16-17 -0.6501332378851217 1101 18-19 -0.639527764307033 1101 20-21 -0.6290734363624679 1101 22-23 -0.6101553238055786 1101 24-25 -0.6749386752012043 1101 26-27 -0.7342840532241013 1101 28-29 -0.744116813846567 1101 30-31 -0.7856298123710772 1101 32-33 -0.7657675927268386 1101 34-35 -0.8224940618574053 1101 36-37 -0.8101758299516817 1101 38-39 -0.7955827211856885 1101 40-41 -1.0134477238461557 1101 42-43 -1.0598702580660557 1101 44-45 -1.0750466106429712 1101 46-47 -1.0697110996238877 1101 48-49 -1.1316920217279502 1101 50-51 -0.9497948258294144 1101 52-53 -0.7595121968904408 1101 54-55 -0.747696893922658 1101 56-57 -0.8289746384634711 1101 58-59 -0.7608102317429797 1101 60-61 -0.7679895757749833 1101 62-63 -0.8761281653656994 1101 64-65 -0.8176681489517748 1101 66-67 -0.8316458519831187 1101 68-69 -0.8837529395001837 1101 70-71 -0.8879583308504237 1101 72-73 -0.9750315709792048 1101 74-75 -1.0062284937987584 1101 76-77 -0.9567555156654173 1101 78-79 -0.9792328894854521 1101 80-81 -1.0920571326324513 1101 82-83 -1.1099479284575544 1101 84-85 -1.095324757345356 1101 86-87 -1.210517021197056 1101 88-89 -1.300989223402162 1101 90-91 -1.3881968249657461 1101 92-93 -1.4611720301157334 1101 94-95 -1.5251638978584303 1101 96-97 -1.623741163767363 1101 98-99 -1.6333025871901405 1101 100 -1.607143289267139 1102 1 -0.3809532577521004 1102 2 -0.36578600977118114 1102 3 -0.4513562326148879 1102 4 -0.4106407669948311 1102 5 -0.2645680404636508 1102 6 -0.2979344678024205 1102 7 -0.402788447276059 1102 8 -0.3367194047036861 1102 9 -0.4902642256083354 1102 10-11 -0.42583442818305883 1102 12-13 -0.5189382707305441 1102 14-15 -0.659093302662157 1102 16-17 -0.6996873670297887 1102 18-19 -0.6791384563510334 1102 20-21 -0.7109476912047583 1102 22-23 -0.7369474984611628 1102 24-25 -0.7288417726102665 1102 26-27 -0.8023727006372638 1102 28-29 -0.8387635295150773 1102 30-31 -0.7460020255037492 1102 32-33 -0.8088220665992054 1102 34-35 -0.8240613915511759 1102 36-37 -0.8381473377581656 1102 38-39 -0.8851033605259815 1102 40-41 -1.0463778323984556 1102 42-43 -1.0529511805969847 1102 44-45 -1.084420338032274 1102 46-47 -1.153778676811143 1102 48-49 -1.1244667576067116 1102 50-51 -1.0073906537295585 1102 52-53 -0.8276094030176253 1102 54-55 -0.8143992020244539 1102 56-57 -0.7831135901831274 1102 58-59 -0.7953348578221195 1102 60-61 -0.8486348371459016 1102 62-63 -0.8482836226487223 1102 64-65 -0.9307847079447882 1102 66-67 -0.9400192502137159 1102 68-69 -0.9273465078094034 1102 70-71 -0.9203406591002548 1102 72-73 -0.9124967943160911 1102 74-75 -1.006475213070459 1102 76-77 -1.0046742720682786 1102 78-79 -1.065855936492305 1102 80-81 -1.0372342348326065 1102 82-83 -1.0873322853119305 1102 84-85 -1.1766809768777868 1102 86-87 -1.1544969686810838 1102 88-89 -1.2499676181111106 1102 90-91 -1.2869208752641086 1102 92-93 -1.2739410567190887 1102 94-95 -1.278478538892518 1102 96-97 -1.413561302717028 1102 98-99 -1.5137339699990449 1102 100 -1.532400409820525 1103 1 -0.46260003961795704 1103 2 -0.5166746192943243 1103 3 -0.5731020781978415 1103 4 -0.3620593000680117 1103 5 -0.3909180523519211 1103 6 -0.4003561861702494 1103 7 -0.45919773828141786 1103 8 -0.45495240089913125 1103 9 -0.5747168522252437 1103 10-11 -0.6033235637912142 1103 12-13 -0.684457769163771 1103 14-15 -0.7354104940404369 1103 16-17 -0.7752209977960334 1103 18-19 -0.7984838993266479 1103 20-21 -0.7768689456395279 1103 22-23 -0.7849084480415343 1103 24-25 -0.8220816837415796 1103 26-27 -0.8228736941957706 1103 28-29 -0.8842547777968406 1103 30-31 -0.8853398241026866 1103 32-33 -0.9479931011787315 1103 34-35 -0.9193882492246175 1103 36-37 -0.9542901662937666 1103 38-39 -0.9678122738617034 1103 40-41 -0.9780256138156602 1103 42-43 -1.0090308580310179 1103 44-45 -1.056331876068704 1103 46-47 -1.0577140519895991 1103 48-49 -1.05356240334946 1103 50-51 -1.0129112364010666 1103 52-53 -0.8233756485029602 1103 54-55 -0.8678598277150158 1103 56-57 -0.8769466224126674 1103 58-59 -0.7954066239900541 1103 60-61 -0.8383959199220001 1103 62-63 -0.8848913548262587 1103 64-65 -0.8513077508077558 1103 66-67 -0.9597316226000174 1103 68-69 -0.9122422541570927 1103 70-71 -0.8760184420860995 1103 72-73 -0.8205725841680689 1103 74-75 -0.9636389752593715 1103 76-77 -1.0076187616533403 1103 78-79 -0.9786963344578794 1103 80-81 -1.0087608967525235 1103 82-83 -0.9850018440417436 1103 84-85 -1.0465494788498724 1103 86-87 -1.1587423711370484 1103 88-89 -1.1360669861763775 1103 90-91 -1.1169391434676186 1103 92-93 -1.2271256108488728 1103 94-95 -1.1825585517470607 1103 96-97 -1.305745878546091 1103 98-99 -1.3958059360549164 1103 100 -1.3401720703341198 1104 1 -0.537605529466191 1104 2 -0.49580689211100903 1104 3 -0.5109013478344337 1104 4 -0.36324061749530756 1104 5 -0.4047896113098659 1104 6 -0.37978587855284474 1104 7 -0.40956633334782566 1104 8 -0.4718359733310393 1104 9 -0.5859784330716664 1104 10-11 -0.5606755862328967 1104 12-13 -0.5883653130394109 1104 14-15 -0.7585752833916288 1104 16-17 -0.7848347509415916 1104 18-19 -0.7859065595805603 1104 20-21 -0.7898507851988441 1104 22-23 -0.8109110455227437 1104 24-25 -0.8247382396954137 1104 26-27 -0.8742842828878565 1104 28-29 -0.9403391599480244 1104 30-31 -0.8888718782713028 1104 32-33 -0.9927588625070882 1104 34-35 -0.9447528422613729 1104 36-37 -1.0237170793635926 1104 38-39 -1.0369576601623152 1104 40-41 -1.0282197756665141 1104 42-43 -1.0732741773083276 1104 44-45 -1.1656855833630644 1104 46-47 -1.1409323891710486 1104 48-49 -1.1364277169677877 1104 50-51 -1.1323294057991546 1104 52-53 -0.9453827691744578 1104 54-55 -0.8784157024109405 1104 56-57 -0.8708793497594556 1104 58-59 -0.8726484823220986 1104 60-61 -0.8900661658083777 1104 62-63 -0.9022018122681885 1104 64-65 -0.927773684161437 1104 66-67 -0.9124327884393679 1104 68-69 -0.958633324925799 1104 70-71 -0.8996756863618671 1104 72-73 -1.0048869582460043 1104 74-75 -1.0201958721839688 1104 76-77 -1.0568300317879675 1104 78-79 -0.9772825491874109 1104 80-81 -0.9398077681056627 1104 82-83 -0.9846123693389579 1104 84-85 -1.0226370377862146 1104 86-87 -1.068638802052913 1104 88-89 -1.1176256313640565 1104 90-91 -1.121877977656883 1104 92-93 -1.1915749936941253 1104 94-95 -1.1031071289519936 1104 96-97 -1.1738967727472414 1104 98-99 -1.2784429661292727 1104 100 -1.2430848687348224 1105 1 -0.49846709300780034 1105 2 -0.44257616906239505 1105 3 -0.4643914938292184 1105 4 -0.3733732602050708 1105 5 -0.3637552541331033 1105 6 -0.33835531334096913 1105 7 -0.404589625384979 1105 8 -0.3562356884328963 1105 9 -0.49284827172321855 1105 10-11 -0.4903744703461044 1105 12-13 -0.5615980104955796 1105 14-15 -0.7195325406732493 1105 16-17 -0.7242642438573057 1105 18-19 -0.6804850208320374 1105 20-21 -0.7363240282540033 1105 22-23 -0.7315259926412878 1105 24-25 -0.718631903787113 1105 26-27 -0.7255685468601385 1105 28-29 -0.7314260433721103 1105 30-31 -0.7454629215982109 1105 32-33 -0.8355398762518362 1105 34-35 -0.8271979772593667 1105 36-37 -0.8231125796148078 1105 38-39 -0.8390684391535927 1105 40-41 -0.8376161055630718 1105 42-43 -0.7410054923299825 1105 44-45 -0.7711120573183337 1105 46-47 -0.9374184375040144 1105 48-49 -0.8575583506883433 1105 50-51 -0.8478812579914603 1105 52-53 -0.7561516171554032 1105 54-55 -0.6992372227191694 1105 56-57 -0.6733989577040305 1105 58-59 -0.6370524192320843 1105 60-61 -0.6490925980339384 1105 62-63 -0.7136882171109491 1105 64-65 -0.7138466114001858 1105 66-67 -0.6443662973491087 1105 68-69 -0.6533932810448562 1105 70-71 -0.6695059196354762 1105 72-73 -0.6838161247206642 1105 74-75 -0.7675626334728776 1105 76-77 -0.8306412849115645 1105 78-79 -0.8608488370029193 1105 80-81 -0.9076039306069319 1105 82-83 -0.8414635221553333 1105 84-85 -0.7789214117516146 1105 86-87 -0.8250363744466434 1105 88-89 -0.8691566838283826 1105 90-91 -0.8047354360485812 1105 92-93 -0.8852492097435736 1105 94-95 -1.0648697616207308 1105 96-97 -1.1103809028738993 1105 98-99 -1.0566010047902914 1105 100 -1.1188645995911166 1106 1 -0.4627240804654207 1106 2 -0.49751780375220633 1106 3 -0.5299305692774112 1106 4 -0.35758791116241895 1106 5 -0.37409967068413863 1106 6 -0.4462667814893635 1106 7 -0.46562256640589084 1106 8 -0.46520052945025725 1106 9 -0.6407585518304444 1106 10-11 -0.7060871365565546 1106 12-13 -0.6466978870979432 1106 14-15 -0.8448684648354927 1106 16-17 -0.8059425530598077 1106 18-19 -0.8536429371120775 1106 20-21 -0.857800832938608 1106 22-23 -0.7453619999340972 1106 24-25 -0.8152544793396501 1106 26-27 -0.8384218565997017 1106 28-29 -0.8991862314999608 1106 30-31 -0.931444349652665 1106 32-33 -0.9996600206091557 1106 34-35 -0.8824990652780542 1106 36-37 -0.9658002342234084 1106 38-39 -1.0218563368978764 1106 40-41 -0.9749682014483056 1106 42-43 -0.9698119710463473 1106 44-45 -0.9986230338524109 1106 46-47 -0.9798699321901907 1106 48-49 -0.9908391072764857 1106 50-51 -0.8978951250730347 1106 52-53 -0.8451491479622604 1106 54-55 -0.8119163835318304 1106 56-57 -0.8828683441045371 1106 58-59 -0.8582567973649304 1106 60-61 -0.794900481501081 1106 62-63 -0.8621045847200364 1106 64-65 -0.8293999213492569 1106 66-67 -0.7955094815643804 1106 68-69 -0.7011298416627767 1106 70-71 -0.8018984908989424 1106 72-73 -0.7492475358931117 1106 74-75 -0.7274585441607826 1106 76-77 -0.8627363007495283 1106 78-79 -0.8689241736342979 1106 80-81 -0.9150804676018289 1106 82-83 -0.8632724134249408 1106 84-85 -0.8605764979702251 1106 86-87 -0.8797075963551073 1106 88-89 -0.9025492786796541 1106 90-91 -0.9015069362815922 1106 92-93 -0.9099200523502482 1106 94-95 -0.9550762962911428 1106 96-97 -0.9743966855598032 1106 98-99 -1.0196988182577584 1106 100 -1.042215921738233 1107 1 -0.4931899805372062 1107 2 -0.4943082237259233 1107 3 -0.49551011898024555 1107 4 -0.35952554198792797 1107 5 -0.34724458710908124 1107 6 -0.43346526170804367 1107 7 -0.41959199978403205 1107 8 -0.44187400462825366 1107 9 -0.5555706928976107 1107 10-11 -0.5289831916221459 1107 12-13 -0.6328666915988705 1107 14-15 -0.7614398566000276 1107 16-17 -0.8289320490587002 1107 18-19 -0.8603231029885805 1107 20-21 -0.8052061101265124 1107 22-23 -0.7401179077965523 1107 24-25 -0.817058093821359 1107 26-27 -0.848594833657387 1107 28-29 -0.8816270497465979 1107 30-31 -0.8860671484799525 1107 32-33 -0.9052518510210774 1107 34-35 -0.845901695173005 1107 36-37 -0.8546974273036199 1107 38-39 -0.8686202331442487 1107 40-41 -0.8433718251717437 1107 42-43 -0.8365647928533733 1107 44-45 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22-23 1.1690615269274929 2310 24-25 1.2093348728284212 2310 26-27 1.237155330480661 2310 28-29 1.241260987737789 2310 30-31 1.2951668702991839 2310 32-33 1.3068317819293327 2310 34-35 1.314506023203684 2310 36-37 1.3136451061890355 2310 38-39 1.3532210908150901 2310 40-41 1.3118199486481217 2310 42-43 1.380497139994283 2310 44-45 1.4249518751116597 2310 46-47 1.4954483530462 2310 48-49 1.519686613086364 2310 50-51 1.481378877601287 2310 52-53 1.364834801256393 2310 54-55 1.3569441759404057 2310 56-57 1.2923103975531873 2310 58-59 1.2839598149563471 2310 60-61 1.3431730723478452 2310 62-63 1.29279244893862 2310 64-65 1.2763509768888568 2310 66-67 1.2365142556310076 2310 68-69 1.2619075756434626 2310 70-71 1.2382713614733518 2310 72-73 1.1908350606096434 2310 74-75 1.1654183020185513 2310 76-77 1.1847947055329087 2310 78-79 1.2172467665622762 2310 80-81 1.2799835776565978 2310 82-83 1.2760686081551889 2310 84-85 1.3045390527435252 2310 86-87 1.32610476587055 2310 88-89 1.3822455654764845 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1.2730692531501973 2311 50-51 1.2467188068755064 2311 52-53 1.1623904841124215 2311 54-55 1.0705899727303603 2311 56-57 1.031537520843699 2311 58-59 1.0716486748325664 2311 60-61 1.0731912644738628 2311 62-63 1.048583954444375 2311 64-65 1.0319226670395025 2311 66-67 1.029234592770031 2311 68-69 1.0050782012068709 2311 70-71 1.0042323986465718 2311 72-73 1.0445644464440633 2311 74-75 1.0231743795060524 2311 76-77 0.9864439127914082 2311 78-79 1.035695565867158 2311 80-81 1.0131149325037079 2311 82-83 1.0846421471231942 2311 84-85 0.9972223099186017 2311 86-87 1.0771168046468667 2311 88-89 1.1187505309351273 2311 90-91 1.1131270828199291 2311 92-93 1.1570022838974268 2311 94-95 1.204667594431637 2311 96-97 1.2278246285237415 2311 98-99 1.2763303970047524 2311 100 1.4647938844994677 2312 1 0.6162395630987056 2312 2 0.629430865848807 2312 3 0.6666205526119207 2312 4 0.5459202834426407 2312 5 0.5757607366652095 2312 6 0.4241665182502672 2312 7 0.5748426950237544 2312 8 0.616878906145601 2312 9 0.8216377918944673 2312 10-11 0.8314395716517353 2312 12-13 0.8453388374357402 2312 14-15 1.0515742688719598 2312 16-17 1.0683362540623236 2312 18-19 1.0476465892709186 2312 20-21 1.0709069584589983 2312 22-23 1.0749231877935088 2312 24-25 1.101321005060612 2312 26-27 1.1584930102829034 2312 28-29 1.1812194099161815 2312 30-31 1.1229065871497568 2312 32-33 1.1819272755216161 2312 34-35 1.2238777984779716 2312 36-37 1.202199819328051 2312 38-39 1.2614297124240395 2312 40-41 1.2870058470543597 2312 42-43 1.338740116544848 2312 44-45 1.352331317579747 2312 46-47 1.3075375713380524 2312 48-49 1.378178416487728 2312 50-51 1.2948739334726795 2312 52-53 1.171163431476586 2312 54-55 1.1685785085146492 2312 56-57 1.1217642717844782 2312 58-59 1.1431583998664365 2312 60-61 1.1372396023227935 2312 62-63 1.1437122058057483 2312 64-65 1.1580831474831115 2312 66-67 1.1142130094592204 2312 68-69 1.14039850538272 2312 70-71 1.194498848116389 2312 72-73 1.2115558075664623 2312 74-75 1.1882573559678917 2312 76-77 1.167489486928801 2312 78-79 1.1336445849552277 2312 80-81 0.9269729724706615 2312 82-83 1.101424840333717 2312 84-85 1.1963001815958343 2312 86-87 1.192253266988967 2312 88-89 1.2393162118762113 2312 90-91 1.2987237262056084 2312 92-93 1.3971941155638703 2312 94-95 1.4018557365134505 2312 96-97 1.457611494404489 2312 98-99 1.3416094924562252 2312 100 1.5100339999072894 2313 1 0.5716866389108546 2313 2 0.5950530280364319 2313 3 0.6250073303237649 2313 4 0.5317842598312694 2313 5 0.5135331850253948 2313 6 0.5571105556523221 2313 7 0.621167744681479 2313 8 0.6148877815288927 2313 9 0.7666476650350873 2313 10-11 0.6735950614714454 2313 12-13 0.7538990932601664 2313 14-15 1.0139304302942165 2313 16-17 0.9806316791925767 2313 18-19 0.9489343580177305 2313 20-21 0.9539082259233354 2313 22-23 0.9960489548030154 2313 24-25 1.0563558474690353 2313 26-27 1.0825534412834372 2313 28-29 1.0657540581958926 2313 30-31 1.1087576013184872 2313 32-33 1.1624993833307684 2313 34-35 1.1217358005837994 2313 36-37 1.1472886068115429 2313 38-39 1.1772289981975135 2313 40-41 1.1650799866588528 2313 42-43 1.1949034727350138 2313 44-45 1.1714270085259244 2313 46-47 1.1907090108478329 2313 48-49 1.2391411252495743 2313 50-51 1.1933103087668258 2313 52-53 1.0663306778605204 2313 54-55 1.0366419851582052 2313 56-57 1.0159313530874599 2313 58-59 1.0609644074671252 2313 60-61 1.0572050451702424 2313 62-63 1.0535998780413038 2313 64-65 1.0644166331720868 2313 66-67 1.0247733150245324 2313 68-69 1.0655364203081703 2313 70-71 1.0456967457941957 2313 72-73 1.035775114480188 2313 74-75 1.0276282022073673 2313 76-77 1.021754271943351 2313 78-79 1.0375949271114138 2313 80-81 1.0766004940648237 2313 82-83 1.0940268068315682 2313 84-85 1.1403302395188284 2313 86-87 1.1914675901442955 2313 88-89 1.2372098148809627 2313 90-91 1.2684464172788807 2313 92-93 1.3066614656997189 2313 94-95 1.3602699374670983 2313 96-97 1.3850636321448135 2313 98-99 1.4547559992496453 2313 100 1.550449360295488 2314 1 0.49516784094073785 2314 2 0.5083089364803541 2314 3 0.5493759315173996 2314 4 0.4764214220267533 2314 5 0.4832700206714975 2314 6 0.5126607621283128 2314 7 0.5372069711607921 2314 8 0.5396450415276135 2314 9 0.6765937726833329 2314 10-11 0.6853068399287991 2314 12-13 0.45116831465265506 2314 14-15 0.84679901750102 2314 16-17 0.851274956569462 2314 18-19 0.910541849774809 2314 20-21 0.9094934102733632 2314 22-23 0.8276051586700603 2314 24-25 0.8238920902633851 2314 26-27 0.9195838651360191 2314 28-29 0.969786182386045 2314 30-31 0.9326132721169813 2314 32-33 0.9863784734401833 2314 34-35 1.028968569071104 2314 36-37 1.1290735632304703 2314 38-39 1.1547395988969456 2314 40-41 1.1617291658377553 2314 42-43 1.2533640301258373 2314 44-45 1.275084840941318 2314 46-47 1.3006581397259396 2314 48-49 1.325809172653436 2314 50-51 1.2546843718186267 2314 52-53 1.0805241693975205 2314 54-55 1.0076635927919213 2314 56-57 1.0099377162206196 2314 58-59 1.0316938642079378 2314 60-61 1.0417279734249192 2314 62-63 1.0156162225556855 2314 64-65 0.9740889938980786 2314 66-67 1.0072494941780405 2314 68-69 1.0344326275749722 2314 70-71 1.0435781091216185 2314 72-73 1.0450729401419423 2314 74-75 1.0628026226979799 2314 76-77 1.0905128214565067 2314 78-79 1.0795524048829037 2314 80-81 1.116872307190281 2314 82-83 1.0022193908771975 2314 84-85 1.0470976127992948 2314 86-87 1.1785188650465592 2314 88-89 1.2949119843719252 2314 90-91 1.357477960349435 2314 92-93 1.3993570594058653 2314 94-95 1.4464974104334694 2314 96-97 1.4478077264267242 2314 98-99 1.4595639082337009 2314 100 1.414839789581972 2315 1 0.4496648811159858 2315 2 0.47708342118827574 2315 3 0.529184935584496 2315 4 0.3973588364220433 2315 5 0.37453274422810523 2315 6 -0.3089064175655025 2315 7 0.2558453890941337 2315 8 0.30970652713344293 2315 9 0.557046920702561 2315 10-11 0.6173008789281411 2315 12-13 0.6662711485401971 2315 14-15 0.779298761974097 2315 16-17 0.654736205981088 2315 18-19 0.7784430766867771 2315 20-21 0.7915070671653197 2315 22-23 0.7091555418381077 2315 24-25 0.7941236725793317 2315 26-27 0.841829641395897 2315 28-29 0.8957540265745934 2315 30-31 0.9568481892546146 2315 32-33 1.0009923122960203 2315 34-35 0.964258635915634 2315 36-37 1.0197650350506748 2315 38-39 1.0715869556413082 2315 40-41 1.086668773898964 2315 42-43 1.134889715823931 2315 44-45 1.1482089035799063 2315 46-47 1.1441417461985992 2315 48-49 1.1930043809611988 2315 50-51 1.1356115555453812 2315 52-53 0.9737697872979396 2315 54-55 0.8203632698179817 2315 56-57 0.7836541648184792 2315 58-59 0.7471262460911561 2315 60-61 0.7060693944671073 2315 62-63 0.882786086854928 2315 64-65 0.950052462416771 2315 66-67 0.9084139726774083 2315 68-69 0.9469832999218184 2315 70-71 1.0214366687069472 2315 72-73 1.0190425155726004 2315 74-75 1.0160657637363855 2315 76-77 0.9820962387692802 2315 78-79 0.9509492670417998 2315 80-81 0.9828719321189858 2315 82-83 1.1046064671405595 2315 84-85 1.029547446163999 2315 86-87 1.0378840677960035 2315 88-89 1.1528319093311943 2315 90-91 1.22585219548861 2315 92-93 1.3352651260645736 2315 94-95 1.3917742399015545 2315 96-97 1.404529554398529 2315 98-99 1.4905137962400126 2315 100 1.5824665675474918 2316 1 0.07822622524359346 2316 2 -0.01103122987630556 2316 3 0.12428221890692015 2316 4 0.04511682318620558 2316 5 0.0773265974001518 2316 6 0.1447382740029397 2316 7 0.14543034508739083 2316 8 0.17270604047698868 2316 9 0.3130295534966976 2316 10-11 0.16456814830515754 2316 12-13 0.24808160380620592 2316 14-15 0.46812520843631233 2316 16-17 0.4858055708232101 2316 18-19 0.4519357362306309 2316 20-21 0.4021649209823508 2316 22-23 0.30948631270204885 2316 24-25 0.35401678990322694 2316 26-27 0.46805592240564664 2316 28-29 0.5564282820310353 2316 30-31 0.5654402692500469 2316 32-33 0.660898124876141 2316 34-35 0.6322627914743251 2316 36-37 0.6790819349245183 2316 38-39 0.7499100678185684 2316 40-41 0.7285116665725369 2316 42-43 0.7659803238429888 2316 44-45 0.6593731419994953 2316 46-47 0.5912281893632141 2316 48-49 0.7140558404568367 2316 50-51 0.5229121221870159 2316 52-53 0.2626169182981428 2316 54-55 0.18313786916362318 2316 56-57 0.34742335451313267 2316 58-59 0.36887289674530166 2316 60-61 0.4311460948493462 2316 62-63 0.48765545353236206 2316 64-65 0.457617621020475 2316 66-67 0.48328675792724596 2316 68-69 0.5109867559751677 2316 70-71 0.3467879662002744 2316 72-73 0.4563257107776124 2316 74-75 0.5651204721569485 2316 76-77 0.655497963810582 2316 78-79 0.7301225591663467 2316 80-81 0.7759933854692953 2316 82-83 0.7845246499235294 2316 84-85 0.8138503979406728 2316 86-87 0.8892502036931056 2316 88-89 0.9846078690148055 2316 90-91 0.9754172883665788 2316 92-93 1.048455445146626 2316 94-95 1.070535238608553 2316 96-97 1.1609164376991217 2316 98-99 1.3258422571691604 2316 100 1.2167437980974576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 74.0 1 797.0 2 399.0 3 279.0 4 280.0 5 190.0 6 277.0 7 1170.0 8 3838.0 9 7160.0 10 10752.0 11 15894.0 12 22082.0 13 27904.0 14 33338.0 15 39481.0 16 48520.0 17 60642.0 18 74339.0 19 89234.0 20 106526.0 21 127007.0 22 138834.0 23 142770.0 24 146190.0 25 155570.0 26 173566.0 27 203896.0 28 242147.0 29 288714.0 30 347892.0 31 430761.0 32 544255.0 33 706400.0 34 968064.0 35 1324250.0 36 1943073.0 37 2822743.0 38 2795691.0 39 805694.0 40 929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41969868521713 19.781671856199498 12.950592044011936 23.848037414571436 2 15.290852811890854 20.81116636141177 40.95925044385642 22.938730382840955 3 17.57224823880427 27.699020117781448 30.385211916291265 24.343519727123013 4 11.944907744616598 17.48598229345876 39.97272393898648 30.596386022938155 5 12.662675273398854 38.25890067161041 35.48725919008163 13.591164864909096 6 29.03524146450307 38.51377552780275 19.316742552122747 13.134240455571437 7 25.933844901927944 32.836712850853374 24.30446474686733 16.924977500351357 8 25.31634619912636 35.36240079727994 21.36671690424927 17.95453609934443 9 26.65196892146981 15.385823221139585 21.181889749936023 36.780318107454576 10-11 24.311840100025563 26.16130388095848 29.554394573986514 19.97246144502944 12-13 25.08609904543589 24.237114108663995 29.117908530721003 21.558878315179104 14-15 22.559246015063064 25.438324121729543 26.816773127517813 25.185656735689577 16-17 21.894211781033604 27.95908275140636 27.514504708427935 22.6322007591321 18-19 21.615849325247293 27.605571987579268 29.25760517566151 21.520973511511926 20-21 22.737476742495318 26.878785795107135 28.675581456979334 21.708156005418214 22-23 22.591908472758295 26.84722626037761 28.39640971336718 22.164455553496907 24-25 22.180538704688285 26.88533003061947 28.78727970262106 22.146851562071188 26-27 22.13265630343453 27.030779906602415 28.534792560034234 22.301771229928818 28-29 21.96469567396457 27.42761731610227 28.531770253407963 22.075916756525196 30-31 22.62423244769647 27.01723498566695 28.42930326374996 21.929229302886622 32-33 22.516936683428998 27.172060889927373 28.180114264839723 22.1308881618039 34-35 22.59068909701583 27.16557662518956 28.489877931316492 21.753856346478116 36-37 22.481996242998726 27.053463453558628 28.387803700589682 22.07673660285296 38-39 22.382773888018715 27.08451009947368 28.526185141426442 22.00653087108116 40-41 22.37489039400776 26.650166430715693 28.752419380013045 22.2225237952635 42-43 22.20893987749672 26.879596680706243 28.376845874821672 22.534617566975363 44-45 22.054848587918414 27.23685997763469 28.45383367190923 22.254457762537662 46-47 22.044926627565914 26.898078189044682 28.23685039709545 22.820144786293955 48-49 22.267747406673276 26.84564090670666 28.29484705372474 22.591764632895327 50-51 22.17561926945662 27.068989926461267 27.988900962820868 22.766489841261244 52-53 21.860543069309198 27.300764650296035 27.93354860735188 22.905143673042886 54-55 21.842609484195634 27.125259056764605 28.082531312137082 22.949600146902682 56-57 22.152994279996506 26.981959014803785 27.966784060353035 22.898262644846678 58-59 21.968760819523666 26.947526049126864 28.210042255992303 22.87367087535717 60-61 22.053814523501607 27.193263964441723 27.862638657076307 22.890282854980367 62-63 22.096876038981886 26.87499471721963 27.843109892237344 23.185019351561145 64-65 21.795068795471987 26.691985116453115 27.95333449782577 23.559611590249123 66-67 21.992611644859725 26.59970174198924 27.7835466677605 23.624139945390535 68-69 21.78059615561752 26.761800951522467 27.861398272925932 23.59620461993408 70-71 21.97100985563868 26.43692685945716 27.899798340413174 23.692264944490987 72-73 22.168662922421962 26.581298049066156 27.762510486333824 23.487528542178058 74-75 22.236698755854746 26.706503812286066 27.606075761878003 23.45072166998119 76-77 22.09433966266755 26.578641062034308 27.60233159212917 23.724687683168973 78-79 21.960540036855804 26.56011793965627 27.737053048769546 23.74228897471838 80-81 22.05937904567919 26.662587889081408 27.68083880805229 23.59719425718712 82-83 22.443811180270462 26.35116928898332 27.841002801670918 23.364016729075303 84-85 22.607044451539938 26.48554100103904 27.372246516039056 23.53516803138197 86-87 22.16768881451658 26.673142929718104 27.623750194702048 23.535418061063275 88-89 22.425447989557952 26.143725374923104 27.602135026092828 23.828691609426116 90-91 22.71397617270646 26.01173598795207 27.619369821679847 23.65491801766163 92-93 22.857499984173653 26.07019772426572 27.449281948577443 23.623020342983185 94-95 22.520019269845797 25.975084706300866 27.58969870088831 23.915197322965025 96-97 22.676779607451454 25.824337295883325 27.50996137688159 23.988921719783626 98-99 22.60005866439822 25.96566078439891 27.339391055464123 24.09488949573875 100 23.024439336222745 25.641113891079932 27.101878354740112 24.232568417957218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 701.0 1 563.0 2 1017.0 3 1554.0 4 1261.5 5 1178.0 6 1622.0 7 2802.5 8 4565.5 9 5714.5 10 5765.5 11 5837.0 12 6709.5 13 7828.5 14 8997.0 15 10262.0 16 11454.5 17 12712.0 18 14187.0 19 15640.0 20 17360.0 21 19748.5 22 23144.5 23 28340.0 24 35632.0 25 45573.0 26 59175.5 27 75785.0 28 96911.5 29 125486.0 30 158582.5 31 190660.0 32 230451.0 33 270607.0 34 307727.0 35 345131.0 36 383634.5 37 434436.5 38 464724.0 39 468136.0 40 470140.5 41 472123.0 42 481740.0 43 500393.0 44 522646.0 45 554263.0 46 579819.0 47 597833.5 48 613649.0 49 615612.5 50 607330.5 51 598061.0 52 580061.5 53 551232.0 54 517203.0 55 465392.5 56 411753.5 57 354558.5 58 297332.0 59 254974.5 60 209454.5 61 164584.0 62 129764.0 63 101440.0 64 75411.0 65 53595.0 66 38982.0 67 29715.5 68 23078.5 69 17823.5 70 14192.5 71 10833.5 72 8304.5 73 6558.5 74 5151.5 75 4097.0 76 3072.5 77 2189.5 78 1569.0 79 1131.5 80 837.5 81 599.5 82 428.0 83 319.0 84 246.0 85 189.5 86 134.0 87 102.5 88 80.5 89 78.5 90 68.0 91 51.0 92 47.5 93 33.0 94 21.0 95 19.5 96 14.0 97 8.0 98 4.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21794925833690085 2 0.1477212387980249 3 0.02432730916528848 4 0.023142253418515498 5 0.028111407629415833 6 0.05519935802298227 7 0.06173063117281062 8 0.054896360815000544 9 0.04215701153719102 10-11 0.044550689480246666 12-13 0.042853905115549 14-15 0.020533110794228402 16-17 0.01900802484738704 18-19 0.01843233015222176 20-21 0.021472402138971756 22-23 0.024717838900020484 24-25 0.021728266447934106 26-27 0.01914942354444518 28-29 0.01897772512658887 30-31 0.017186674963852434 32-33 0.02523293415358942 34-35 0.025535931361571146 36-37 0.02430710935142303 38-39 0.022711324056052598 40-41 0.023913212981046785 42-43 0.029811558629757744 44-45 0.03946706965744213 46-47 0.044705554719881774 48-49 0.041173953929072526 50-51 0.02833360558193576 52-53 0.04488398640902657 54-55 0.043227601672059794 56-57 0.04511965090412347 58-59 0.04860411879591333 60-61 0.024643772915847174 62-63 0.0259466609101686 64-65 0.026606521496439915 66-67 0.03194937226385105 68-69 0.0364572973914903 70-71 0.04080025737256173 72-73 0.04514995062492164 74-75 0.025842295205197117 76-77 0.01884305970081921 78-79 0.017994667518470373 80-81 0.02167440027762624 82-83 0.020085348253544293 84-85 0.012264653651971482 86-87 0.013527142018562012 88-89 0.018923858956281004 90-91 0.019768884502985598 92-93 0.01995741609906312 94-95 0.023923312887979508 96-97 0.0245730735673181 98-99 0.03001019013276799 100 0.030521918750692684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.4851622E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.71673766505264 #Duplication Level Percentage of deduplicated Percentage of total 1 72.49332219330232 41.84078059498969 2 15.354846630862188 17.724633097611804 3 5.524805292200666 9.566212131013211 4 2.4823123339390953 5.730838791227494 5 1.310496512637848 3.781879171544251 6 0.7803753413112047 2.7024431312840833 7 0.4740692873373807 1.9153212884617046 8 0.31718257193803123 1.464539463717923 9 0.23976584186163136 1.2454651976191449 >10 0.9661076331131242 9.278002904205158 >50 0.038562829230582255 1.5119031244836603 >100 0.01693599039150161 1.7516067849840147 >500 7.473890882332732E-4 0.30171066955144565 >1k 4.2151659120336635E-4 0.44621264430658103 >5k 0.0 0.0 >10k+ 4.863619506666182E-5 0.7384510049999072 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35541 0.23930719486396837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25164 0.16943603870338203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18214 0.12263980324842634 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.3466542576965668E-5 0.0 2 0.0 0.0 0.0 2.0199813865448502E-5 0.0 3 0.0 0.0 0.0 2.0199813865448502E-5 0.0 4 0.0 0.0 0.0 2.6933085153931335E-5 1.3466542576965668E-5 5 0.0 0.0 0.0 4.0399627730897004E-5 1.3466542576965668E-5 6 1.3466542576965668E-5 0.0 0.0 4.0399627730897004E-5 1.3466542576965668E-5 7 1.3466542576965668E-5 0.0 0.0 4.0399627730897004E-5 1.3466542576965668E-5 8 1.3466542576965668E-5 0.0 0.0 4.0399627730897004E-5 1.3466542576965668E-5 9 1.3466542576965668E-5 0.0 0.0 5.386617030786267E-5 2.0199813865448502E-5 10-11 1.3466542576965668E-5 0.0 0.0 8.753252675027683E-5 2.0199813865448502E-5 12-13 1.3466542576965668E-5 6.733271288482834E-6 0.0 1.1109897625996676E-4 4.0399627730897004E-5 14-15 2.0199813865448502E-5 6.733271288482834E-6 0.0 1.21198883192691E-4 6.05994415963455E-5 16-17 2.0199813865448502E-5 6.733271288482834E-6 0.0 1.4813196834662234E-4 9.763243368300108E-5 18-19 2.0199813865448502E-5 6.733271288482834E-6 0.0 1.9189823172176076E-4 1.0773234061572534E-4 20-21 2.0199813865448502E-5 6.733271288482834E-6 0.0 3.1646375055869317E-4 1.3129879012541525E-4 22-23 2.0199813865448502E-5 6.733271288482834E-6 0.0 9.157248952336653E-4 1.5823187527934658E-4 24-25 2.0199813865448502E-5 6.733271288482834E-6 0.0 0.002511510190604097 1.6833178221207083E-4 26-27 2.0199813865448502E-5 6.733271288482834E-6 0.0 0.004797455793044019 1.6833178221207083E-4 28-29 2.0199813865448502E-5 6.733271288482834E-6 0.0 0.012668649929280452 1.7169841785631224E-4 30-31 2.0199813865448502E-5 6.733271288482834E-6 0.0 0.045176883710075576 1.7506505350055367E-4 32-33 2.0199813865448502E-5 6.733271288482834E-6 0.0 0.11874797244368326 1.7506505350055367E-4 34-35 2.0199813865448502E-5 1.3466542576965668E-5 0.0 0.2159225436790675 1.952648673660022E-4 36-37 2.0199813865448502E-5 2.0199813865448502E-5 0.0 0.3668555528816987 1.952648673660022E-4 38-39 2.0199813865448502E-5 3.366635644241417E-5 0.0 0.6299816949286752 2.0873140994296784E-4 40-41 2.0199813865448502E-5 4.376626337513842E-5 0.0 0.9487751573531834 2.188313168756921E-4 42-43 2.0199813865448502E-5 5.386617030786267E-5 0.0 1.2660199673813406 2.2556458816417495E-4 44-45 2.0199813865448502E-5 5.386617030786267E-5 0.0 1.6110159550249796 2.3229785945265777E-4 46-47 2.0199813865448502E-5 5.386617030786267E-5 0.0 2.0044645628605413 2.3566449509689917E-4 48-49 2.0199813865448502E-5 5.386617030786267E-5 0.0 2.437626004755575 2.491310376738648E-4 50-51 2.6933085153931335E-5 5.386617030786267E-5 0.0 2.9048005665643792 2.558643089623477E-4 52-53 2.6933085153931335E-5 5.386617030786267E-5 0.0 3.3675480025010063 2.82797394116279E-4 54-55 2.6933085153931335E-5 5.386617030786267E-5 0.0 3.845226467519844 3.130971149144518E-4 56-57 2.6933085153931335E-5 5.386617030786267E-5 0.0 4.363654690376579 3.1646375055869317E-4 58-59 2.6933085153931335E-5 5.386617030786267E-5 0.0 4.938002731284166 3.1646375055869317E-4 60-61 2.6933085153931335E-5 5.386617030786267E-5 0.0 5.5342709368714065 3.2319702184717603E-4 62-63 2.6933085153931335E-5 5.386617030786267E-5 0.0 6.14160527382127 3.3666356442414166E-4 64-65 2.6933085153931335E-5 5.386617030786267E-5 0.0 6.722410521894512 3.3666356442414166E-4 66-67 2.6933085153931335E-5 6.05994415963455E-5 0.0 7.336303065079356 3.3666356442414166E-4 68-69 2.6933085153931335E-5 6.05994415963455E-5 0.0 8.003617382667024 3.3666356442414166E-4 70-71 2.6933085153931335E-5 6.05994415963455E-5 0.0 8.703756397786046 3.400302000683831E-4 72-73 3.366635644241417E-5 6.05994415963455E-5 0.0 9.396764878610565 4.0399627730897006E-4 74-75 3.366635644241417E-5 6.05994415963455E-5 0.0 10.066136210576865 4.174628198859357E-4 76-77 3.366635644241417E-5 6.733271288482834E-5 0.0 10.752431619926767 4.2419609117441855E-4 78-79 3.366635644241417E-5 6.733271288482834E-5 0.0 11.476951810381383 4.3429599810714274E-4 80-81 3.366635644241417E-5 7.069934852906975E-5 0.0 12.227371528847153 4.477625406841085E-4 82-83 3.366635644241417E-5 8.753252675027683E-5 0.0 12.993486502686373 4.7132899019379835E-4 84-85 3.366635644241417E-5 9.426579803875967E-5 0.0 13.727584771548859 4.7132899019379835E-4 86-87 3.366635644241417E-5 9.426579803875967E-5 0.0 14.46070334943887 4.814288971265226E-4 88 3.366635644241417E-5 9.426579803875967E-5 0.0 15.017948881273709 4.915288040592469E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18945 0.0 49.391773 1 GTATCAA 35430 0.0 43.804874 1 CAACGCA 43085 0.0 35.089375 5 ATCAACG 43145 0.0 35.062485 3 TATCAAC 45630 0.0 33.73235 2 TCAACGC 45870 0.0 33.102283 4 AACGCAG 46765 0.0 32.830757 6 TACCTGG 10425 0.0 32.328552 2 ACGCAGA 51025 0.0 29.537483 7 CGCAGAG 52335 0.0 28.714869 8 GTACATG 47000 0.0 27.046686 1 TACATGG 47055 0.0 26.348707 2 ACATGGG 47370 0.0 25.415188 3 GCAGAGT 59010 0.0 24.669497 9 ACCTGGG 13670 0.0 23.762749 3 CATGGGG 36350 0.0 23.35595 4 ATGGGGG 27135 0.0 22.192434 5 AGAGTAC 54410 0.0 21.437695 10-11 GTACTAG 6240 0.0 20.613323 1 TAACGCA 1530 0.0 20.58579 4 >>END_MODULE