FastQCFastQC Report
Mon 6 Jun 2016
ERR1211375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14851622
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313650.21118905396326407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233370.15713435205932388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173820.11703772153640862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188950.049.0984341
GTATCAA342400.041.4038731
ATCAACG427300.032.1933823
CAACGCA429300.032.178895
TATCAAC456050.030.8639052
TCAACGC455950.030.3149934
AACGCAG466650.030.1463626
TACCTGG103300.028.3435482
ACGCAGA511000.027.0701897
CGCAGAG524100.026.2949478
GTACATG456900.025.1928731
TACATGG457800.024.648072
ACATGGG458050.023.670723
GTACTAG61400.023.0507761
GCAGAGT596400.022.610799
CATGGGG356400.021.9429654
ACCTGGG128800.021.747393
ATGGGGG261150.021.3752425
AGAGTAC551650.021.32281110-11
TATCACG11750.020.7984982
Produced by FastQC (version 0.11.5)