FastQCFastQC Report
Mon 6 Jun 2016
ERR1211374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19250848
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674240.3502391167391691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492880.2560302798089726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344690.17905185267682752No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC213710.11101329146643306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA356550.050.250521
GTATCAA677300.043.495271
CAACGCA826700.034.4861455
ATCAACG838750.034.0134663
TATCAAC887600.033.0437362
AACGCAG914900.031.865856
GTACTAG135050.030.6291261
TCAACGC946600.030.3365734
ACGCAGA976200.029.1197
CGCAGAG990200.028.6348978
GTACATG995400.027.901071
TAGGCAT169200.027.670745
TACATGG1004700.027.2297042
GCAGAGT1054750.026.5395769
ACATGGG1006950.026.5299263
TATTCTC103900.026.3763545
GCATCGT148100.025.8360798
GCTATTG218050.025.54588
CTATTGA219300.025.4004549
AGGGCTA220750.025.340085
Produced by FastQC (version 0.11.5)