##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211374_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19250848 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64714297261087 31.0 31.0 34.0 30.0 34.0 2 31.729858913228135 33.0 31.0 34.0 30.0 34.0 3 31.79436469499941 33.0 31.0 34.0 30.0 34.0 4 35.43391917072952 37.0 35.0 37.0 33.0 37.0 5 35.48707449147175 37.0 35.0 37.0 33.0 37.0 6 35.54287125429487 37.0 35.0 37.0 33.0 37.0 7 35.47618499714922 37.0 35.0 37.0 33.0 37.0 8 35.486282786088175 37.0 35.0 37.0 33.0 37.0 9 37.095996498439966 39.0 37.0 39.0 33.0 39.0 10-11 37.02829090957448 39.0 37.0 39.0 33.0 39.0 12-13 36.93920779489818 39.0 37.0 39.0 33.0 39.0 14-15 38.15411295128401 40.0 38.0 41.0 33.0 41.0 16-17 38.16278615362814 40.0 38.0 41.0 33.0 41.0 18-19 38.17139660548979 40.0 38.0 41.0 33.0 41.0 20-21 38.15732478382251 40.0 38.0 41.0 33.0 41.0 22-23 38.02828896160834 40.0 38.0 41.0 33.0 41.0 24-25 37.92515703723805 40.0 37.5 41.0 33.0 41.0 26-27 37.77821397789853 40.0 37.0 41.0 32.5 41.0 28-29 37.677614305613965 40.0 37.0 41.0 32.0 41.0 30-31 37.51768968826724 40.0 37.0 41.0 32.0 41.0 32-33 37.33630986022018 40.0 36.0 41.0 31.5 41.0 34-35 37.21621696872782 39.5 36.0 41.0 31.0 41.0 36-37 37.082044255920565 39.0 36.0 41.0 31.0 41.0 38-39 36.91983605085865 39.0 35.5 41.0 30.5 41.0 40-41 36.74334616324434 39.0 35.0 41.0 30.0 41.0 42-43 36.53761320020811 39.0 35.0 40.0 30.0 41.0 44-45 36.35575284787454 39.0 35.0 40.0 29.5 41.0 46-47 36.09141711575511 38.5 35.0 40.0 28.5 41.0 48-49 35.81358519894812 38.0 35.0 40.0 27.5 41.0 50-51 35.11105365332478 37.5 34.0 39.5 26.5 40.5 52-53 35.39759765387997 38.0 34.5 39.5 28.0 40.5 54-55 35.779690172609534 38.0 35.0 40.0 28.0 41.0 56-57 35.65490543585405 38.0 35.0 40.0 28.0 41.0 58-59 35.411257415777214 38.0 34.0 40.0 27.5 41.0 60-61 35.17480414369278 37.0 34.0 40.0 27.0 41.0 62-63 34.88504358353461 37.0 34.0 40.0 26.5 41.0 64-65 34.66324551001597 36.0 34.0 40.0 26.0 41.0 66-67 34.46041101669911 36.0 34.0 39.5 26.0 41.0 68-69 34.138826949337506 35.5 33.0 39.0 26.0 41.0 70-71 33.81875663347402 35.0 33.0 39.0 25.5 41.0 72-73 33.470010905493616 35.0 33.0 38.0 25.0 40.0 74-75 33.099589041480144 35.0 33.0 37.0 24.0 40.0 76-77 32.75606396663669 35.0 32.0 37.0 24.0 39.0 78-79 32.40822087421812 35.0 32.0 36.0 23.0 39.0 80-81 32.062991069276535 35.0 32.0 36.0 22.5 38.5 82-83 31.7214352843054 35.0 31.0 35.5 20.5 37.0 84-85 31.41830375472291 35.0 31.0 35.0 20.0 37.0 86-87 31.123313269108976 34.5 31.0 35.0 19.5 36.5 88-89 30.865775029754534 34.0 31.0 35.0 19.0 36.0 90-91 30.601832449147174 34.0 30.0 35.0 18.0 36.0 92-93 30.33882008730213 34.0 30.0 35.0 17.5 35.5 94-95 30.091317873373683 34.0 29.0 35.0 16.0 35.0 96-97 29.832635346764985 34.0 29.0 35.0 15.0 35.0 98-99 29.563361598408548 34.0 29.0 35.0 11.5 35.0 100 28.301108761546505 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3152885437170312 1101 2 -0.3030826853817885 1101 3 -0.3675920902913994 1101 4 -0.2396898498798592 1101 5 -0.22731735025431732 1101 6 -0.2806125982936649 1101 7 -0.28785280527552715 1101 8 -0.3011951169610185 1101 9 -0.4263396275202922 1101 10-11 -0.3758960926954771 1101 12-13 -0.40954812135358765 1101 14-15 -0.6085705623959967 1101 16-17 -0.570403024286513 1101 18-19 -0.6042799226882991 1101 20-21 -0.6213642546728977 1101 22-23 -0.6185461514387214 1101 24-25 -0.6588066660980729 1101 26-27 -0.6982848574546878 1101 28-29 -0.71709279943898 1101 30-31 -0.780250852842876 1101 32-33 -0.7570062897238685 1101 34-35 -0.8053943334299447 1101 36-37 -0.8572382675805912 1101 38-39 -0.8198393785544624 1101 40-41 -1.013181799238346 1101 42-43 -0.9773822860550183 1101 44-45 -0.9505626703085213 1101 46-47 -1.014389641235553 1101 48-49 -1.064565644771605 1101 50-51 -0.9461149113527796 1101 52-53 -0.7376911831104991 1101 54-55 -0.7480935952957921 1101 56-57 -0.758580243747673 1101 58-59 -0.7528989106367732 1101 60-61 -0.7625803426968432 1101 62-63 -0.8259055477745676 1101 64-65 -0.7763490770065076 1101 66-67 -0.8687574729378227 1101 68-69 -0.9148689835253165 1101 70-71 -0.8945747724232476 1101 72-73 -0.9612597432857015 1101 74-75 -0.9900610407865145 1101 76-77 -0.9713810259841296 1101 78-79 -1.0645337515296092 1101 80-81 -1.1756617170111099 1101 82-83 -1.1587254561066942 1101 84-85 -1.1670299097214105 1101 86-87 -1.2283091772591739 1101 88-89 -1.3406726807923803 1101 90-91 -1.4483519261202247 1101 92-93 -1.5356159350236425 1101 94-95 -1.5887980152312515 1101 96-97 -1.7587988403883585 1101 98-99 -1.8305120179120635 1101 100 -1.915287591890099 1102 1 -0.3707240828711953 1102 2 -0.4035568515998875 1102 3 -0.46353427955060766 1102 4 -0.3385960554706102 1102 5 -0.2706561408940473 1102 6 -0.3304424725514252 1102 7 -0.3992014008805853 1102 8 -0.3468289858022757 1102 9 -0.5228705206617477 1102 10-11 -0.4871992815437025 1102 12-13 -0.5296847576897434 1102 14-15 -0.6822800218325966 1102 16-17 -0.7183334177489655 1102 18-19 -0.7366750373332209 1102 20-21 -0.7616865459142801 1102 22-23 -0.7121050449116524 1102 24-25 -0.7523052372225223 1102 26-27 -0.7947207050501888 1102 28-29 -0.7904020393144222 1102 30-31 -0.7837386357234379 1102 32-33 -0.8652804955747797 1102 34-35 -0.89048299110042 1102 36-37 -0.9378467185784913 1102 38-39 -1.0122085765400541 1102 40-41 -1.0849495527245807 1102 42-43 -1.1236523165310572 1102 44-45 -1.101954610332804 1102 46-47 -1.2159247350886702 1102 48-49 -1.2302042578563075 1102 50-51 -1.0638030391695565 1102 52-53 -0.9093558027685233 1102 54-55 -0.8945077951580416 1102 56-57 -0.8548416410082282 1102 58-59 -0.8237702750350593 1102 60-61 -0.8435691746879428 1102 62-63 -0.8966258920112367 1102 64-65 -0.9933983172186842 1102 66-67 -0.955730437685709 1102 68-69 -0.948147879810783 1102 70-71 -0.9981396079497884 1102 72-73 -0.9868335591162989 1102 74-75 -1.098515602588467 1102 76-77 -1.1156688634701268 1102 78-79 -1.168702842068182 1102 80-81 -1.1717737047918817 1102 82-83 -1.2148019729711947 1102 84-85 -1.2983338004644942 1102 86-87 -1.311111831887061 1102 88-89 -1.3781934830154405 1102 90-91 -1.4484634581777662 1102 92-93 -1.494829149477141 1102 94-95 -1.5218638455575224 1102 96-97 -1.6401762172676584 1102 98-99 -1.7474764352909986 1102 100 -1.6018755811927967 1103 1 -0.3977888797806628 1103 2 -0.4561007174912497 1103 3 -0.47946495727109806 1103 4 -0.32466142479925253 1103 5 -0.2949125285454173 1103 6 -0.274895224732731 1103 7 -0.3719133369171601 1103 8 -0.3571006007108579 1103 9 -0.4370203520254847 1103 10-11 -0.42739182448931246 1103 12-13 -0.5268370209651394 1103 14-15 -0.6469402603207115 1103 16-17 -0.682806312323514 1103 18-19 -0.6691078933675314 1103 20-21 -0.6509841412538577 1103 22-23 -0.6858321842544939 1103 24-25 -0.661410732831051 1103 26-27 -0.7142345918686672 1103 28-29 -0.7436643510573404 1103 30-31 -0.7484760095890337 1103 32-33 -0.8232352746654286 1103 34-35 -0.7943930790913925 1103 36-37 -0.8095030617838361 1103 38-39 -0.8058804123444006 1103 40-41 -0.8495298355271075 1103 42-43 -0.867212170386729 1103 44-45 -0.8336325997696363 1103 46-47 -0.9491388386391364 1103 48-49 -0.9689215083654972 1103 50-51 -0.9420822721904045 1103 52-53 -0.7782390663817012 1103 54-55 -0.7594573469092154 1103 56-57 -0.7358172943276742 1103 58-59 -0.6951201017175208 1103 60-61 -0.7329711693615195 1103 62-63 -0.7399614245805353 1103 64-65 -0.6855424072255758 1103 66-67 -0.7085963609790724 1103 68-69 -0.7234774608876151 1103 70-71 -0.7607957013969511 1103 72-73 -0.8022279498231697 1103 74-75 -0.8780771898200399 1103 76-77 -0.9561433860299395 1103 78-79 -0.9685140164249404 1103 80-81 -0.9822408503009292 1103 82-83 -0.9836725251437244 1103 84-85 -1.0432176583738801 1103 86-87 -1.1887542162531943 1103 88-89 -1.19331468796506 1103 90-91 -1.2100155798631995 1103 92-93 -1.2719396708231727 1103 94-95 -1.2777473876439238 1103 96-97 -1.410830514039322 1103 98-99 -1.476734071146364 1103 100 -1.579490206605584 1104 1 -0.4189227092339607 1104 2 -0.39848451406006546 1104 3 -0.42617096404707766 1104 4 -0.21987237527675774 1104 5 -0.26342169582706276 1104 6 -0.265143254038712 1104 7 -0.2601231455965305 1104 8 -0.36822070431796305 1104 9 -0.42218757724863565 1104 10-11 -0.42577193826686965 1104 12-13 -0.49145184592333635 1104 14-15 -0.6351515923081266 1104 16-17 -0.6391699906155281 1104 18-19 -0.6667512176088763 1104 20-21 -0.6551987712531115 1104 22-23 -0.6472803049808675 1104 24-25 -0.6715253484414987 1104 26-27 -0.6405087858150793 1104 28-29 -0.7459281208093742 1104 30-31 -0.7827869110137087 1104 32-33 -0.7572631307028459 1104 34-35 -0.7763117105986055 1104 36-37 -0.8583984490824292 1104 38-39 -0.8360769083551602 1104 40-41 -0.827521537386275 1104 42-43 -0.8044348920443269 1104 44-45 -0.8771084506873663 1104 46-47 -0.7966937444961673 1104 48-49 -0.780594416563261 1104 50-51 -0.7678428130431598 1104 52-53 -0.635876008577597 1104 54-55 -0.5773728014188393 1104 56-57 -0.5279938841200291 1104 58-59 -0.5519658546601249 1104 60-61 -0.6369035850445144 1104 62-63 -0.6212594460894323 1104 64-65 -0.6120495217038737 1104 66-67 -0.6092508103845375 1104 68-69 -0.6740852670775794 1104 70-71 -0.6449933811556079 1104 72-73 -0.6733184303877451 1104 74-75 -0.6854591318925785 1104 76-77 -0.7308694385347323 1104 78-79 -0.7399060520673473 1104 80-81 -0.786917910751658 1104 82-83 -0.792681422374006 1104 84-85 -0.8523977159645888 1104 86-87 -0.8754483340753723 1104 88-89 -0.9119225602283905 1104 90-91 -0.9441182893057274 1104 92-93 -1.0545172617455556 1104 94-95 -1.136467355472071 1104 96-97 -1.15905031406038 1104 98-99 -1.227463220788625 1104 100 -1.1079825271117478 1105 1 -0.4428996599420856 1105 2 -0.4597891129825449 1105 3 -0.45360024894116435 1105 4 -0.30793222819367827 1105 5 -0.3492026030215669 1105 6 -0.3214155130202201 1105 7 -0.3244743151134699 1105 8 -0.35553133339121956 1105 9 -0.4464249443871111 1105 10-11 -0.44537789458461674 1105 12-13 -0.5128127769649709 1105 14-15 -0.6321156787348414 1105 16-17 -0.6128555335295118 1105 18-19 -0.5935234545412058 1105 20-21 -0.6851284579632164 1105 22-23 -0.6651524994378377 1105 24-25 -0.6600502060358977 1105 26-27 -0.690239009197029 1105 28-29 -0.7060801463704607 1105 30-31 -0.7446123165535212 1105 32-33 -0.8322119386637254 1105 34-35 -0.7975904556288071 1105 36-37 -0.7929543823608682 1105 38-39 -0.8258577688554354 1105 40-41 -0.8362039331669706 1105 42-43 -0.7868235503532475 1105 44-45 -0.8692095633407675 1105 46-47 -0.9011538076167795 1105 48-49 -0.9171963243227665 1105 50-51 -0.928019642066161 1105 52-53 -0.8003261197067246 1105 54-55 -0.7325434864422604 1105 56-57 -0.6792314422058894 1105 58-59 -0.6172701501460267 1105 60-61 -0.6789282777973469 1105 62-63 -0.7416083547179753 1105 64-65 -0.7908964258148714 1105 66-67 -0.7701858561898263 1105 68-69 -0.7921494105956484 1105 70-71 -0.7449260315009312 1105 72-73 -0.7666561078313023 1105 74-75 -0.7534378243130462 1105 76-77 -0.8269751764459912 1105 78-79 -0.911921558976978 1105 80-81 -0.9071471796543626 1105 82-83 -0.9612006991104174 1105 84-85 -0.9121628187761317 1105 86-87 -0.9638133183294961 1105 88-89 -0.950424945436044 1105 90-91 -1.053260901455669 1105 92-93 -1.0258018873456507 1105 94-95 -1.1188590570632613 1105 96-97 -1.159117365868621 1105 98-99 -1.193421082721354 1105 100 -1.222272096327064 1106 1 -0.45496864744141874 1106 2 -0.4914811679855049 1106 3 -0.5381395556259498 1106 4 -0.3421554511033307 1106 5 -0.33026076949416705 1106 6 -0.35690760519286613 1106 7 -0.3541697721307173 1106 8 -0.3304377466681174 1106 9 -0.523529051411451 1106 10-11 -0.6053713757470192 1106 12-13 -0.5452633033564283 1106 14-15 -0.6928889248788224 1106 16-17 -0.6715156728588454 1106 18-19 -0.706375222544736 1106 20-21 -0.766845912108451 1106 22-23 -0.6664830280885425 1106 24-25 -0.70772280130128 1106 26-27 -0.7549696718970651 1106 28-29 -0.7421007600132157 1106 30-31 -0.740943234872411 1106 32-33 -0.8531992255597274 1106 34-35 -0.8161965348615112 1106 36-37 -0.8172559266174062 1106 38-39 -0.9430136732465755 1106 40-41 -0.9232000640295084 1106 42-43 -0.8854143222186863 1106 44-45 -0.8895789400600904 1106 46-47 -0.8947693522399689 1106 48-49 -0.8584837179456386 1106 50-51 -0.8703933962103818 1106 52-53 -0.8158608478012255 1106 54-55 -0.729350720267675 1106 56-57 -0.7856929348304149 1106 58-59 -0.7425490152791028 1106 60-61 -0.6643008173343006 1106 62-63 -0.74965658010467 1106 64-65 -0.7390465375741471 1106 66-67 -0.7391336098016978 1106 68-69 -0.7082952215147245 1106 70-71 -0.7932834832859683 1106 72-73 -0.7801150423962397 1106 74-75 -0.7295071828130304 1106 76-77 -0.7999985089762021 1106 78-79 -0.7828687215812238 1106 80-81 -0.8504895235839847 1106 82-83 -0.8554034264744068 1106 84-85 -0.7958454515980478 1106 86-87 -0.8274795602846723 1106 88-89 -0.8459315273472363 1106 90-91 -0.8520901324090637 1106 92-93 -0.8838693850984782 1106 94-95 -0.9137624735285605 1106 96-97 -0.9625134165609062 1106 98-99 -1.0161300437537086 1106 100 -1.1008059309478817 1107 1 -0.49888448518672845 1107 2 -0.48968055985050896 1107 3 -0.48840549136669154 1107 4 -0.33667418998832943 1107 5 -0.31999891321285645 1107 6 -0.36763104855045725 1107 7 -0.36377964947915586 1107 8 -0.39810616434480295 1107 9 -0.5551498801780639 1107 10-11 -0.5113121931772397 1107 12-13 -0.5646836784540099 1107 14-15 -0.7099067238695156 1107 16-17 -0.7581877432148332 1107 18-19 -0.6993946896209309 1107 20-21 -0.7073540836902055 1107 22-23 -0.7442609415825601 1107 24-25 -0.8123107859351464 1107 26-27 -0.8191258659083331 1107 28-29 -0.831003635267642 1107 30-31 -0.8856964593942465 1107 32-33 -0.8543519226901353 1107 34-35 -0.9002178107457581 1107 36-37 -0.8800038807270809 1107 38-39 -0.848101346817387 1107 40-41 -0.9302632047255486 1107 42-43 -0.8534250736784372 1107 44-45 -0.8722483910546188 1107 46-47 -0.8101592730966587 1107 48-49 -0.9306463208765052 1107 50-51 -0.9566814584060808 1107 52-53 -0.8026350199543302 1107 54-55 -0.7254509009776484 1107 56-57 -0.6805460531505005 1107 58-59 -0.7011218741383942 1107 60-61 -0.6864499715447465 1107 62-63 -0.655827134052295 1107 64-65 -0.692594789631265 1107 66-67 -0.6607651954092546 1107 68-69 -0.6465872583646401 1107 70-71 -0.6223915666246427 1107 72-73 -0.6432539190765993 1107 74-75 -0.5430525149304444 1107 76-77 -0.6522082503341196 1107 78-79 -0.7073884722165786 1107 80-81 -0.6680991324705126 1107 82-83 -0.7168749116637088 1107 84-85 -0.7264560760359302 1107 86-87 -0.8426837892901204 1107 88-89 -0.7896748230063544 1107 90-91 -0.8179180719826675 1107 92-93 -0.8290955847604273 1107 94-95 -0.8583476061282873 1107 96-97 -0.850836730547698 1107 98-99 -0.8380267472455536 1107 100 -0.9560204833089969 1108 1 -0.4631370766221572 1108 2 -0.45890696956590205 1108 3 -0.4863826622192349 1108 4 -0.3263514258961635 1108 5 -0.3203301946699 1108 6 -0.3565131454163506 1108 7 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44-45 1.2263835819092392 2311 46-47 1.2274926120443581 2311 48-49 1.2373709469283227 2311 50-51 1.201603809874996 2311 52-53 1.1109435827394378 2311 54-55 1.0344001804330105 2311 56-57 1.027474178593664 2311 58-59 1.0330507937063516 2311 60-61 0.9881500100520313 2311 62-63 0.9533743830273309 2311 64-65 0.9388284095804309 2311 66-67 0.9683076822777252 2311 68-69 0.9841356840563193 2311 70-71 0.9731135572962373 2311 72-73 1.0035356398170663 2311 74-75 0.9897626109211117 2311 76-77 1.0050449892694004 2311 78-79 1.0146499669171547 2311 80-81 0.9930122316798027 2311 82-83 1.0779899091608733 2311 84-85 1.0461143225942457 2311 86-87 1.120428740397685 2311 88-89 1.1448804991179777 2311 90-91 1.2019184319113592 2311 92-93 1.2382919252287294 2311 94-95 1.2619499839529986 2311 96-97 1.315664436188392 2311 98-99 1.3950260395796548 2311 100 1.4825101748078566 2312 1 0.49101200421748104 2312 2 0.5169254177618576 2312 3 0.5836292681931745 2312 4 0.43245502986513884 2312 5 0.4343620150467302 2312 6 0.3012277223333726 2312 7 0.44558720853333256 2312 8 0.47415844069573865 2312 9 0.6488578628540935 2312 10-11 0.6741895840096461 2312 12-13 0.7307169671305545 2312 14-15 0.8988717978395542 2312 16-17 0.89438966963165 2312 18-19 0.8647763491324483 2312 20-21 0.872566415237614 2312 22-23 0.9311722069107873 2312 24-25 0.9573324901826652 2312 26-27 0.9607257625735599 2312 28-29 0.9995342272982768 2312 30-31 0.8880052391020641 2312 32-33 1.0081007663857164 2312 34-35 1.0386698636982743 2312 36-37 1.0464128913780684 2312 38-39 1.1040017267146496 2312 40-41 1.1463907557905344 2312 42-43 1.1597124975093678 2312 44-45 1.1793531264138295 2312 46-47 1.1190599001245118 2312 48-49 1.1862190907986516 2312 50-51 1.1195336056042606 2312 52-53 1.001183254336695 2312 54-55 0.9615604646917859 2312 56-57 0.9166779236802824 2312 58-59 0.9484418770388743 2312 60-61 0.9003278558678502 2312 62-63 0.9264980679788763 2312 64-65 0.9035601404346139 2312 66-67 0.9281239588730585 2312 68-69 0.9523302638036952 2312 70-71 0.9835459554216257 2312 72-73 1.0207170209913485 2312 74-75 1.0173582598743494 2312 76-77 1.0091605867745201 2312 78-79 1.0266128989865635 2312 80-81 0.8643138522715681 2312 82-83 1.0472753087657019 2312 84-85 1.1440974574717835 2312 86-87 1.1666849509129875 2312 88-89 1.1914601543045542 2312 90-91 1.2463931763328624 2312 92-93 1.2671840830561152 2312 94-95 1.3483865258198868 2312 96-97 1.3681326718864426 2312 98-99 1.213730204388522 2312 100 1.3782059017369441 2313 1 0.5206596978055913 2313 2 0.5354634264827034 2313 3 0.5823035545136896 2313 4 0.44890407266761656 2313 5 0.461192769219835 2313 6 0.48416740774402456 2313 7 0.5274568090606024 2313 8 0.5323079965024107 2313 9 0.7044809339594025 2313 10-11 0.6144729312234389 2313 12-13 0.7095917466788393 2313 14-15 0.8941415642392982 2313 16-17 0.8814470092087845 2313 18-19 0.8874227603024707 2313 20-21 0.9340203323747573 2313 22-23 0.945978131318256 2313 24-25 0.9925700508103503 2313 26-27 1.0249819023222955 2313 28-29 1.0170940537610562 2313 30-31 1.0542862365714214 2313 32-33 1.077283344527551 2313 34-35 1.0574577461826848 2313 36-37 1.0483025451094719 2313 38-39 1.1239430646582989 2313 40-41 1.1257528937201968 2313 42-43 1.1907213163632164 2313 44-45 1.12653738374987 2313 46-47 1.1139098448706548 2313 48-49 1.2291627809356882 2313 50-51 1.1824996627411224 2313 52-53 0.9999703887361804 2313 54-55 0.9470619528216844 2313 56-57 0.9308128819889916 2313 58-59 0.9661824348579415 2313 60-61 0.9512288834934921 2313 62-63 0.9366927985500126 2313 64-65 0.9125969161784369 2313 66-67 0.9301210198558039 2313 68-69 0.9907228055864792 2313 70-71 0.9884516817044187 2313 72-73 1.0138999527523254 2313 74-75 1.0432534631575265 2313 76-77 1.0208868179125261 2313 78-79 1.0675827758385665 2313 80-81 1.0799873241544837 2313 82-83 1.1197634727130215 2313 84-85 1.1933114184460543 2313 86-87 1.2575906976191007 2313 88-89 1.2836601306652575 2313 90-91 1.306564669602782 2313 92-93 1.3793493901396587 2313 94-95 1.477075620663932 2313 96-97 1.4877037886557076 2313 98-99 1.533262618506157 2313 100 1.6462502159958206 2314 1 0.4531210392294369 2314 2 0.4520553533961724 2314 3 0.5370607691822933 2314 4 0.41514056618030537 2314 5 0.4166829939049137 2314 6 0.43606980790099925 2314 7 0.47988689206285784 2314 8 0.4710154028277813 2314 9 0.6165694178726255 2314 10-11 0.5957871191275856 2314 12-13 0.3552154591845209 2314 14-15 0.736999154110201 2314 16-17 0.7602118453737035 2314 18-19 0.7885406958666508 2314 20-21 0.7940750784530337 2314 22-23 0.7334014451074751 2314 24-25 0.7223233514721201 2314 26-27 0.8650511389638069 2314 28-29 0.8799186300841271 2314 30-31 0.815385662408616 2314 32-33 0.8863487583384071 2314 34-35 0.9518094516652127 2314 36-37 1.019826608748879 2314 38-39 1.0225497987507381 2314 40-41 1.0717732933791666 2314 42-43 1.159500972723663 2314 44-45 1.1453274488374845 2314 46-47 1.1292971118923347 2314 48-49 1.1772836749948752 2314 50-51 1.1304560946927253 2314 52-53 0.961113867488578 2314 54-55 0.93850195359785 2314 56-57 0.9085522016616849 2314 58-59 0.9283988380959727 2314 60-61 0.9155893429129236 2314 62-63 0.8974865250870678 2314 64-65 0.8616977582292478 2314 66-67 0.9093956908140797 2314 68-69 0.940626403206565 2314 70-71 0.9647628483809711 2314 72-73 0.9492981702551546 2314 74-75 1.007241097607725 2314 76-77 1.0878682352084468 2314 78-79 1.0961436889332745 2314 80-81 1.1519775252169246 2314 82-83 1.0331955471884662 2314 84-85 1.0672292663654055 2314 86-87 1.2710096470647159 2314 88-89 1.3563569621215485 2314 90-91 1.3982512814335806 2314 92-93 1.450010802455541 2314 94-95 1.4608002447023374 2314 96-97 1.5077602564148975 2314 98-99 1.5732359875916337 2314 100 1.508876907485547 2315 1 0.4219372870028337 2315 2 0.44342471413418494 2315 3 0.48589390024079293 2315 4 0.3971835919741551 2315 5 0.3710304475281134 2315 6 -0.3271298840660819 2315 7 0.2223011342922092 2315 8 0.25685383771654813 2315 9 0.5055846573620784 2315 10-11 0.5579673810965247 2315 12-13 0.6164432229625945 2315 14-15 0.7409344118867622 2315 16-17 0.6176121086447068 2315 18-19 0.7478998126805649 2315 20-21 0.7541634833081758 2315 22-23 0.6382357675118371 2315 24-25 0.7623588141967659 2315 26-27 0.8414414900533842 2315 28-29 0.8893996719463999 2315 30-31 0.931127264674906 2315 32-33 0.9656806647358209 2315 34-35 0.9750925629504081 2315 36-37 1.0275060646627026 2315 38-39 1.030617485649202 2315 40-41 1.0543947435130931 2315 42-43 1.1103345495953292 2315 44-45 1.117356686549094 2315 46-47 1.1542650608578242 2315 48-49 1.1441777251459015 2315 50-51 1.064513550543893 2315 52-53 0.9369851695540348 2315 54-55 0.7874505459790839 2315 56-57 0.7716031060723125 2315 58-59 0.7230074261610611 2315 60-61 0.6449269054832101 2315 62-63 0.8068732811279915 2315 64-65 0.863745491761982 2315 66-67 0.8751807269379697 2315 68-69 0.9548839176978063 2315 70-71 0.9864414220948134 2315 72-73 1.0076284156780773 2315 74-75 1.0237223756131115 2315 76-77 1.0259427429176853 2315 78-79 0.9991011761556692 2315 80-81 1.0454754826810948 2315 82-83 1.1704773490048588 2315 84-85 1.0951629803360383 2315 86-87 1.1747075023191371 2315 88-89 1.3005268559647796 2315 90-91 1.362171452509667 2315 92-93 1.4673569639502944 2315 94-95 1.5158308185760383 2315 96-97 1.5536477951359515 2315 98-99 1.650516654943246 2315 100 1.608978536530877 2316 1 0.11538385813590324 2316 2 -0.0017951944346563664 2316 3 0.15975417901143985 2316 4 0.10646863138815377 2316 5 0.10936135820971771 2316 6 0.16042030285929343 2316 7 0.15222666925981798 2316 8 0.1851236649031094 2316 9 0.3292864142195526 2316 10-11 0.20264427195599666 2316 12-13 0.3018344529396657 2316 14-15 0.5059714631443555 2316 16-17 0.5259971465787743 2316 18-19 0.44986661594755617 2316 20-21 0.4242424620492642 2316 22-23 0.32541691889051094 2316 24-25 0.40890369593447673 2316 26-27 0.5040130417216346 2316 28-29 0.622228842451257 2316 30-31 0.6165180561815546 2316 32-33 0.6905116691776172 2316 34-35 0.7114711261186599 2316 36-37 0.7754345329176857 2316 38-39 0.8059037213119637 2316 40-41 0.7874356408737526 2316 42-43 0.8459034916325976 2316 44-45 0.7078777337741542 2316 46-47 0.7135113322394702 2316 48-49 0.7971556546033653 2316 50-51 0.5574360708933028 2316 52-53 0.30926111048220406 2316 54-55 0.24056854064412647 2316 56-57 0.3849907968611177 2316 58-59 0.4328195740557206 2316 60-61 0.5073688844322533 2316 62-63 0.516575711765924 2316 64-65 0.5623996256588981 2316 66-67 0.5561589170425378 2316 68-69 0.5735978336660921 2316 70-71 0.4576597564316458 2316 72-73 0.5794344547449057 2316 74-75 0.6644177068569306 2316 76-77 0.7333844635677877 2316 78-79 0.8297515367863539 2316 80-81 0.9360209802487027 2316 82-83 0.9685490822143485 2316 84-85 1.042617709207601 2316 86-87 1.0921825085269319 2316 88-89 1.1401901028569164 2316 90-91 1.1506393736008214 2316 92-93 1.2618873237941948 2316 94-95 1.3125903257851554 2316 96-97 1.3863011792267166 2316 98-99 1.511108085802089 2316 100 1.4518542242536085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 66.0 1 1001.0 2 503.0 3 371.0 4 373.0 5 246.0 6 388.0 7 2008.0 8 6556.0 9 12213.0 10 16475.0 11 20229.0 12 23254.0 13 25545.0 14 28420.0 15 33742.0 16 43736.0 17 59762.0 18 79285.0 19 101056.0 20 129536.0 21 165202.0 22 179397.0 23 173493.0 24 166223.0 25 171273.0 26 192824.0 27 229775.0 28 279876.0 29 342255.0 30 425752.0 31 542754.0 32 705656.0 33 942909.0 34 1355624.0 35 1888581.0 36 2790593.0 37 3838589.0 38 3283225.0 39 991562.0 40 520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88849294707385 20.823738170593334 12.810008177275503 23.477760705057314 2 15.57842717058806 20.686378036452176 40.70505249140732 23.030142301552445 3 17.45477990729367 28.70740228532481 29.983393749064437 23.854424058317083 4 11.799664635947424 17.495294090934856 40.46437005037839 30.24067122273933 5 12.575526150010402 38.978139062267154 34.98738832022942 13.458946467493021 6 28.268216952169485 39.10176653942291 19.24369529085498 13.386321217552622 7 26.01981983976782 32.99785692830271 24.318216442081006 16.66410678984846 8 26.287823369195895 34.19501018290244 20.913984683937624 18.603181763964038 9 27.691715665296552 14.21583613891576 21.541723083622855 36.550725112164834 10-11 24.942910825413986 26.08893233557924 28.46518226194659 20.502974577060183 12-13 25.66120333702761 24.40523055529276 28.29228168739462 21.641284420285007 14-15 23.268607468432972 25.10884261227465 26.341308496141657 25.281241423150718 16-17 21.687042664239517 27.5939219147268 27.44758434289814 23.27145107813554 18-19 21.257864994329232 27.534380437993377 29.72190543326352 21.485849134413876 20-21 22.896187825624857 26.465760146306295 28.725048606269567 21.913003421799285 22-23 23.19426521278744 26.240209097735264 28.377938732012964 22.187586957464333 24-25 22.416417922079944 26.56635310560539 29.132355500827416 21.884873471487246 26-27 22.218901093876806 26.735717618305888 28.60313532296227 22.442245964855037 28-29 21.716784439913823 27.30036586436007 28.717294693379543 22.265555002346563 30-31 22.65948492111651 26.758095576146125 28.503108349300042 22.079311153437324 32-33 22.763826103366416 27.17072159692596 27.779274186630342 22.28617811307728 34-35 22.71834135755294 26.855070100394084 28.82078719247935 21.60580134957362 36-37 22.53958520448134 26.72451391198868 28.633339474803503 22.10256140872648 38-39 22.604068450711353 26.572243740430395 28.99734034635197 21.82634746250628 40-41 22.274170195745818 26.016385706831958 29.397183435806785 22.31226066161544 42-43 22.156013146045222 26.30316074734455 28.663936337928153 22.876889768682073 44-45 21.925602494216136 27.14913614150472 28.66115840596884 22.264102958310307 46-47 22.016347408784686 26.6169130959393 28.355341459330674 23.01139803594534 48-49 22.517486649376668 26.359285290126454 28.406329767190947 22.71689829330593 50-51 22.34520691925577 26.52014363852836 27.742568630773906 23.39208081144197 52-53 21.58664639509724 27.061815026095125 27.924810981715908 23.426727597091723 54-55 21.75802472779951 26.917958813123207 27.878647629233765 23.44536882984352 56-57 22.41603036396461 26.44414906484849 27.732494112055207 23.407326459131696 58-59 21.872519376988592 26.485998749170935 28.369115456802586 23.272366417037883 60-61 21.99067448157719 27.14166209648025 27.55526504959572 23.312398372346834 62-63 22.180412959290795 26.43288938695828 27.446054914228863 23.940642739522065 64-65 21.478856334308162 26.32092184025534 27.830082505428205 24.37013932000829 66-67 22.18568741109709 25.952212872355574 27.595806514529393 24.26629320201794 68-69 21.692400732866684 26.22487240360339 27.754194813707546 24.32853204982238 70-71 21.94344442736188 25.800870320381907 27.873920391437085 24.38176486081913 72-73 22.440207941647664 26.068052519908168 27.74773751790803 23.744002020536144 74-75 22.3961217584374 26.335409639031237 27.489984693995783 23.778483908535584 76-77 22.087535805669585 26.119199777672726 27.500318096547666 24.292946320110026 78-79 21.898710442363463 26.168758536519093 27.64130402084889 24.291227000268556 80-81 21.973477310141394 26.37928422763811 27.765748218918535 23.881490243301958 82-83 22.68815961553164 25.553906047230523 28.28098519211897 23.476949145118866 84-85 22.904988591760898 25.98125052130446 27.19554475384 23.918216133094642 86-87 21.97583937749795 26.501987582179943 27.621265813630664 23.90090722669144 88-89 22.718268379699396 25.475392143965575 27.524934967393815 24.28140450894122 90-91 22.88059891790519 25.296608135288036 27.714111083262775 24.108681863543996 92-93 23.27363929041538 25.57138541079184 27.247290296921058 23.907685001871716 94-95 22.428393082673132 25.389826074430122 27.733210643132455 24.44857019976429 96-97 22.69260847125983 25.249580683045643 27.45026143108599 24.60754941460854 98-99 22.54265114170296 25.625610656259763 27.252394905289645 24.57934329674763 100 23.20231273222775 25.082535226080218 26.729851875145883 24.98530016654615 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1125.0 1 854.5 2 1191.0 3 1824.0 4 1573.5 5 1477.5 6 2089.0 7 3773.0 8 6311.0 9 7916.0 10 7841.0 11 8034.5 12 9547.0 13 11414.0 14 13593.0 15 16173.0 16 18428.0 17 20506.0 18 22703.0 19 24531.5 20 26123.0 21 28272.0 22 31199.5 23 35045.0 24 39337.0 25 44857.5 26 54706.5 27 65870.5 28 80954.0 29 115611.5 30 165328.5 31 210455.0 32 278407.0 33 348190.0 34 407695.0 35 466896.0 36 523578.0 37 615832.5 38 645320.5 39 599021.5 40 559040.5 41 524983.0 42 522270.0 43 554339.5 44 602003.0 45 682924.5 46 751319.5 47 800353.0 48 843894.0 49 841004.5 50 816899.5 51 813145.5 52 800939.5 53 773367.5 54 739963.0 55 660431.0 56 583697.0 57 492930.0 58 398744.5 59 353148.5 60 293008.5 61 222157.5 62 172720.0 63 137281.0 64 100928.0 65 65469.0 66 44182.0 67 31569.0 68 23616.0 69 17976.5 70 14163.0 71 10743.0 72 8427.0 73 7296.0 74 5874.0 75 4490.0 76 3386.5 77 2358.0 78 1678.0 79 1241.0 80 888.0 81 664.5 82 541.5 83 428.5 84 304.5 85 232.5 86 181.0 87 122.5 88 94.0 89 80.5 90 74.0 91 56.0 92 42.0 93 32.5 94 25.5 95 20.0 96 19.0 97 13.5 98 8.5 99 8.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21565803231109612 2 0.14439363917890788 3 0.02327689668527849 4 0.022341872939831014 5 0.026991018785250395 6 0.05375867078686612 7 0.06047006344863353 8 0.05331193721959677 9 0.0413644115833235 10-11 0.04345003399330772 12-13 0.041735823793320685 14-15 0.02028741798802837 16-17 0.018542040329859757 18-19 0.017955053200773285 20-21 0.021310749531656997 22-23 0.02456255433526876 24-25 0.021806831574380514 26-27 0.019261489156217948 28-29 0.01844594066713321 30-31 0.01689535962259948 32-33 0.02478332383072164 34-35 0.02515993061708243 36-37 0.02411322347981762 38-39 0.02263276921619245 40-41 0.02369765737072985 42-43 0.029445456117050014 44-45 0.038686607467889204 46-47 0.04368638721785139 48-49 0.04020082647787775 50-51 0.02833381677524024 52-53 0.04396429705330383 54-55 0.042037109222409316 56-57 0.044491546554208936 58-59 0.047842048308729054 60-61 0.024585929928904952 62-63 0.025868990290713424 64-65 0.026409226232527522 66-67 0.03214663582612049 68-69 0.036185418948817216 70-71 0.04002161359333365 72-73 0.04379287603330513 74-75 0.024965134003447537 76-77 0.01842516236167882 78-79 0.017635586754412066 80-81 0.021009464102568366 82-83 0.019212140680763778 84-85 0.011737145293547588 86-87 0.012786449718994196 88-89 0.018596583381677523 90-91 0.019406937294398668 92-93 0.019806919674395643 94-95 0.023585973978912513 96-97 0.024243087888907545 98-99 0.0294610398461408 100 0.029775311716138425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.9250848E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.813657790478096 #Duplication Level Percentage of deduplicated Percentage of total 1 68.0889620231038 32.55582329481545 2 15.850356040788027 15.157269991829523 3 6.520331340334465 9.352826741618445 4 3.2453415406446617 6.206865993504273 5 1.840403161286857 4.399820347514193 6 1.1733535419370924 3.3661394832855316 7 0.7329865785995267 2.45327386039198 8 0.5071020194868688 1.9397121939684399 9 0.3651159463572221 1.5711776021673698 >10 1.5590995123326363 12.4527363628405 >50 0.07217911232089878 2.3686220272087644 >100 0.03923250408517488 3.685430224251448 >500 0.0034118252124055353 1.1299559342206706 >1k 0.001998804389617766 1.6960517501853036 >5k 5.4021051830984874E-5 0.17865363259621372 >10k+ 7.202806910797983E-5 1.485640559602018 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67424 0.3502391167391691 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49288 0.2560302798089726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34469 0.17905185267682752 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 21371 0.11101329146643306 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.194576363597074E-6 0.0 3 0.0 0.0 0.0 5.194576363597074E-6 0.0 4 0.0 0.0 0.0 1.0389152727194147E-5 1.5583729090791223E-5 5 0.0 0.0 0.0 1.5583729090791223E-5 1.5583729090791223E-5 6 0.0 0.0 0.0 1.5583729090791223E-5 3.1167458181582445E-5 7 0.0 0.0 0.0 1.5583729090791223E-5 3.1167458181582445E-5 8 0.0 0.0 0.0 1.5583729090791223E-5 3.636203454517952E-5 9 0.0 0.0 0.0 2.0778305454388295E-5 5.194576363597074E-5 10-11 0.0 0.0 0.0 3.376474636338098E-5 5.194576363597074E-5 12-13 0.0 0.0 0.0 4.4153899090575125E-5 5.4543051817769276E-5 14-15 0.0 0.0 0.0 5.4543051817769276E-5 8.311322181755318E-5 16-17 0.0 0.0 0.0 9.86969509083444E-5 9.869695090834441E-5 18-19 0.0 0.0 0.0 1.4285084999891953E-4 1.2466983272632978E-4 20-21 0.0 0.0 0.0 3.5582848090639954E-4 1.2986440908992685E-4 22-23 0.0 0.0 0.0 0.001088263748173587 1.5064271454431514E-4 24-25 0.0 0.0 0.0 0.002763514625433643 1.7921288454409904E-4 26-27 0.0 0.0 0.0 0.005420540435413547 1.9219932545309172E-4 28-29 0.0 0.0 0.0 0.016749911484418765 1.9219932545309172E-4 30-31 0.0 0.0 0.0 0.06030903158136203 1.9999118999848732E-4 32-33 0.0 0.0 0.0 0.14992066842977514 2.2076949545287564E-4 34-35 0.0 0.0 0.0 0.27413078114792655 2.3375593636186832E-4 36-37 0.0 0.0 0.0 0.4789113705536504 2.3375593636186832E-4 38-39 2.597288181798537E-6 0.0 0.0 0.8164185806256431 2.441450890890625E-4 40-41 5.194576363597074E-6 0.0 0.0 1.199653646426381 2.597288181798537E-4 42-43 5.194576363597074E-6 2.597288181798537E-6 0.0 1.5754994273499017 2.6232610636165224E-4 44-45 5.194576363597074E-6 5.194576363597074E-6 0.0 1.9921927595085682 2.7011797090704785E-4 46-47 5.194576363597074E-6 5.194576363597074E-6 0.0 2.4843840645357544 2.8310441181604053E-4 48-49 5.194576363597074E-6 5.194576363597074E-6 0.0 3.0303937779779884 3.2725831090661564E-4 50-51 5.194576363597074E-6 5.194576363597074E-6 0.0 3.6144277904017525 3.2725831090661564E-4 52-53 5.194576363597074E-6 5.194576363597074E-6 0.0 4.1770289807493155 3.766067863607878E-4 54-55 5.194576363597074E-6 5.194576363597074E-6 0.0 4.737988684965982 4.3114983817855713E-4 56-57 5.194576363597074E-6 5.194576363597074E-6 0.0 5.365384423584873 4.3894170272395274E-4 58-59 5.194576363597074E-6 5.194576363597074E-6 0.0 6.085947486573059 4.571227199965425E-4 60-61 5.194576363597074E-6 5.194576363597074E-6 0.0 6.82682134314291 4.727064490873337E-4 62-63 5.194576363597074E-6 7.791864545395611E-6 0.0 7.556771005620115 4.88290178178125E-4 64-65 5.194576363597074E-6 1.0389152727194147E-5 0.0 8.26564886907839 4.88290178178125E-4 66-67 5.194576363597074E-6 1.0389152727194147E-5 0.0 9.009774530451853 4.93484754541722E-4 68-69 5.194576363597074E-6 1.0389152727194147E-5 0.0 9.80857830262854 4.986793309053191E-4 70-71 5.194576363597074E-6 1.0389152727194147E-5 0.0 10.654785181411228 5.038739072689162E-4 72-73 5.194576363597074E-6 1.0389152727194147E-5 0.0 11.500527145609379 5.869871290864693E-4 74-75 5.194576363597074E-6 1.0389152727194147E-5 0.0 12.287759998936151 5.869871290864693E-4 76-77 5.194576363597074E-6 1.0389152727194147E-5 0.0 13.123616684314374 5.921817054500664E-4 78-79 5.194576363597074E-6 1.0389152727194147E-5 0.0 13.992038168915986 5.973762818136635E-4 80-81 5.194576363597074E-6 1.0389152727194147E-5 0.0 14.905148593973626 6.181545872680518E-4 82-83 5.194576363597074E-6 1.0389152727194147E-5 0.0 15.824715877451215 6.804895036312166E-4 84-85 5.194576363597074E-6 1.5583729090791223E-5 0.0 16.678122439073853 6.856840799948137E-4 86-87 5.194576363597074E-6 2.597288181798537E-5 0.0 17.531928983076487 6.856840799948137E-4 88 5.194576363597074E-6 2.597288181798537E-5 0.0 18.172550113117094 6.908786563584108E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 35655 0.0 50.25052 1 GTATCAA 67730 0.0 43.49527 1 CAACGCA 82670 0.0 34.486145 5 ATCAACG 83875 0.0 34.013466 3 TATCAAC 88760 0.0 33.043736 2 AACGCAG 91490 0.0 31.86585 6 GTACTAG 13505 0.0 30.629126 1 TCAACGC 94660 0.0 30.336573 4 ACGCAGA 97620 0.0 29.119 7 CGCAGAG 99020 0.0 28.634897 8 GTACATG 99540 0.0 27.90107 1 TAGGCAT 16920 0.0 27.67074 5 TACATGG 100470 0.0 27.229704 2 GCAGAGT 105475 0.0 26.539576 9 ACATGGG 100695 0.0 26.529926 3 TATTCTC 10390 0.0 26.376354 5 GCATCGT 14810 0.0 25.836079 8 GCTATTG 21805 0.0 25.5458 8 CTATTGA 21930 0.0 25.400454 9 AGGGCTA 22075 0.0 25.34008 5 >>END_MODULE