FastQCFastQC Report
Mon 6 Jun 2016
ERR1211374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19250848
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586090.3044489260940609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458000.23791159745274598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329690.17125998813143192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA351400.051.620071
GTATCAA659150.043.140551
CAACGCA840850.032.902575
ATCAACG844650.032.664813
TATCAAC896800.031.592612
GTACTAG126300.030.8981761
AACGCAG925700.030.5708396
TCAACGC951550.029.1683034
TAGGCAT161650.027.962755
ACGCAGA1001650.027.633627
TATTCTC106150.027.4443045
CGCAGAG1017950.027.1356748
AGGGCTA222450.026.8046265
GCTATTG220950.026.6878348
GTACATG975250.026.6556171
CTATTGA222750.026.6198279
ATTTAGG227450.026.2941591
TACATGG982800.026.1791042
ACATGGG984700.025.4419023
CTAGGCA177100.025.394684
Produced by FastQC (version 0.11.5)