##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211374_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19250848 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926363399679847 31.0 31.0 34.0 30.0 34.0 2 32.07963638796587 33.0 31.0 34.0 30.0 34.0 3 32.14650284496558 33.0 31.0 34.0 30.0 34.0 4 35.830103640109776 37.0 35.0 37.0 35.0 37.0 5 35.681869390896445 37.0 35.0 37.0 33.0 37.0 6 35.71175929496716 37.0 35.0 37.0 33.0 37.0 7 35.63482071023572 37.0 35.0 37.0 33.0 37.0 8 35.62248426666711 37.0 35.0 37.0 33.0 37.0 9 37.17101485607284 39.0 37.0 39.0 33.0 39.0 10-11 37.13737054596244 39.0 37.0 39.0 33.0 39.0 12-13 37.042760246197986 39.0 37.0 39.0 33.0 39.0 14-15 38.18085146171224 40.0 37.5 41.0 33.0 41.0 16-17 38.1835666408046 40.0 37.5 41.0 33.0 41.0 18-19 38.19805397663521 40.0 37.5 41.0 33.0 41.0 20-21 38.156172782622356 40.0 37.5 41.0 33.0 41.0 22-23 38.04156819481407 40.0 37.0 41.0 33.0 41.0 24-25 37.90268696215357 40.0 37.0 41.0 33.0 41.0 26-27 37.748997135087244 40.0 37.0 41.0 32.5 41.0 28-29 37.69441982503836 40.0 37.0 41.0 32.5 41.0 30-31 37.730536779470704 40.0 37.0 41.0 33.0 41.0 32-33 37.56894823542319 40.0 36.0 41.0 32.0 41.0 34-35 37.44873402459985 40.0 36.0 41.0 32.0 41.0 36-37 37.29658693476776 39.0 36.0 41.0 31.5 41.0 38-39 37.16425372534239 39.0 36.0 41.0 31.0 41.0 40-41 37.083195114313924 39.0 36.0 41.0 31.0 41.0 42-43 36.93045623236961 39.0 35.0 41.0 31.0 41.0 44-45 36.72904990990527 39.0 35.0 41.0 30.5 41.0 46-47 36.464994840746755 39.0 35.0 40.0 30.0 41.0 48-49 36.19695724572756 39.0 35.0 40.0 29.0 41.0 50-51 35.98230576647845 38.0 35.0 40.0 28.5 41.0 52-53 35.74862403463993 38.0 34.5 40.0 28.0 41.0 54-55 35.52359306457565 38.0 34.0 40.0 28.0 41.0 56-57 35.24815106846202 38.0 34.0 40.0 27.0 41.0 58-59 34.94692753275076 37.0 34.0 40.0 26.5 41.0 60-61 34.67248403810575 37.0 33.5 40.0 26.0 41.0 62-63 34.359970324424154 36.0 33.0 39.0 26.0 41.0 64-65 34.00099525485838 35.5 33.0 39.0 26.0 40.5 66-67 33.6528937582386 35.0 32.5 38.5 25.0 40.0 68-69 33.31092035530071 35.0 32.0 38.0 25.0 40.0 70-71 32.99479667077523 35.0 32.0 37.5 24.0 40.0 72-73 32.68286861960574 35.0 31.5 37.0 24.0 39.5 74-75 32.34756577995941 35.0 31.0 36.0 23.0 39.0 76-77 31.410925040808593 34.0 30.5 35.0 22.5 38.0 78-79 31.95619109869861 34.0 31.0 35.5 23.5 38.0 80-81 31.888578648587327 34.0 31.0 35.0 24.0 37.0 82-83 31.64825152117974 34.0 31.0 35.0 23.0 37.0 84-85 31.39929313763217 34.0 31.0 35.0 23.0 36.0 86-87 31.16678078285175 34.0 31.0 35.0 22.0 36.0 88-89 30.956191618156247 34.0 30.0 35.0 20.5 36.0 90-91 30.748090213999923 34.0 30.0 35.0 20.0 35.0 92-93 30.564335945097067 34.0 30.0 35.0 20.0 35.0 94-95 30.410180632042806 34.0 29.5 35.0 19.5 35.0 96-97 30.245431448006862 34.0 29.0 35.0 19.0 35.0 98-99 30.057100861219205 34.0 29.0 35.0 19.0 35.0 100 28.191338324420826 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3268345860053863 1101 2 -0.31418062774430666 1101 3 -0.3868225625739008 1101 4 -0.23373547239578585 1101 5 -0.23909337104756645 1101 6 -0.31092468340366963 1101 7 -0.3031631891017099 1101 8 -0.37738504665995265 1101 9 -0.5279739749119656 1101 10-11 -0.49519683301762285 1101 12-13 -0.5053633280835044 1101 14-15 -0.7086931897238813 1101 16-17 -0.7045907477060283 1101 18-19 -0.6970718747138349 1101 20-21 -0.7565726593038917 1101 22-23 -0.7695456632522948 1101 24-25 -0.832114289270983 1101 26-27 -0.8578813806399026 1101 28-29 -0.8446213330697248 1101 30-31 -0.7978918826790462 1101 32-33 -0.6662163671325061 1101 34-35 -0.831467278674964 1101 36-37 -0.8110277656844787 1101 38-39 -0.8227609112334093 1101 40-41 -0.8692992203914045 1101 42-43 -0.8417670401821695 1101 44-45 -0.8655374045588715 1101 46-47 -0.9001082082084366 1101 48-49 -0.8812863943296634 1101 50-51 -0.9396288569089108 1101 52-53 -0.890058744758015 1101 54-55 -0.9139968996832835 1101 56-57 -0.9565129182660002 1101 58-59 -0.9329955736532938 1101 60-61 -0.9146060033648808 1101 62-63 -0.9323315212528556 1101 64-65 -0.9785208869044553 1101 66-67 -1.0117389566882196 1101 68-69 -1.0215881477949935 1101 70-71 -1.059002594615933 1101 72-73 -1.1082182172221877 1101 74-75 -1.1476730468380651 1101 76-77 -0.943927301964905 1101 78-79 -0.9178987367686382 1101 80-81 -0.7723538996414696 1101 82-83 -0.8666547249527987 1101 84-85 -0.9007979179167975 1101 86-87 -1.0333385970475923 1101 88-89 -0.9998064603454999 1101 90-91 -1.1604140418902738 1101 92-93 -1.28326402811091 1101 94-95 -1.268379912092584 1101 96-97 -1.2198758377685195 1101 98-99 -1.3344544254273814 1101 100 -1.3154905861517925 1102 1 -0.3847499200816209 1102 2 -0.35501484073306244 1102 3 -0.3971469711676754 1102 4 -0.24783465823490047 1102 5 -0.2680691232201724 1102 6 -0.36447418323155745 1102 7 -0.3754125094034322 1102 8 -0.38956960373330674 1102 9 -0.5041747299039159 1102 10-11 -0.5195068584117095 1102 12-13 -0.5377049962509588 1102 14-15 -0.6700752270207673 1102 16-17 -0.7634818088462225 1102 18-19 -0.7861846263593222 1102 20-21 -0.8270680979944061 1102 22-23 -0.8408798053508448 1102 24-25 -0.8526306423814773 1102 26-27 -0.936671974148517 1102 28-29 -0.9460585416620333 1102 30-31 -0.8302072982319331 1102 32-33 -0.8014099860478368 1102 34-35 -0.8557341151774196 1102 36-37 -0.8840616382842157 1102 38-39 -0.9352493412612901 1102 40-41 -0.9350456572499937 1102 42-43 -0.9904638600685232 1102 44-45 -0.9494317149286289 1102 46-47 -1.0176250454556097 1102 48-49 -0.9936048847139176 1102 50-51 -0.9957679612450505 1102 52-53 -0.9730489287701687 1102 54-55 -1.0517145111254393 1102 56-57 -0.9849534984240833 1102 58-59 -1.1126785973360356 1102 60-61 -1.1770757752961742 1102 62-63 -1.1228219625276026 1102 64-65 -1.127234540404011 1102 66-67 -1.1329929587290266 1102 68-69 -1.1520809109676478 1102 70-71 -1.1932026457705618 1102 72-73 -1.2071738513855905 1102 74-75 -1.237241432083124 1102 76-77 -1.0545551663394441 1102 78-79 -0.9856660578542709 1102 80-81 -0.8794722555080448 1102 82-83 -0.9286577540775731 1102 84-85 -0.9884780508591255 1102 86-87 -1.0618643328001731 1102 88-89 -1.0527804265972236 1102 90-91 -1.0609036122496 1102 92-93 -1.1478430527535686 1102 94-95 -1.170989186343796 1102 96-97 -1.2368302390661263 1102 98-99 -1.2511487081280919 1102 100 -1.3413290221449294 1103 1 -0.39511778546314247 1103 2 -0.3325318135079378 1103 3 -0.414963841203317 1103 4 -0.19647708260187358 1103 5 -0.18005405752497694 1103 6 -0.31543961995313197 1103 7 -0.34165202912110004 1103 8 -0.3715087667757544 1103 9 -0.49975180268452135 1103 10-11 -0.4993684972074064 1103 12-13 -0.6154116614026819 1103 14-15 -0.7859635909894607 1103 16-17 -0.8147948363938795 1103 18-19 -0.7893233889447657 1103 20-21 -0.7587106253748388 1103 22-23 -0.8915625972450769 1103 24-25 -0.8317317175508165 1103 26-27 -0.8694120624620254 1103 28-29 -0.8959788975505489 1103 30-31 -0.7691966695051704 1103 32-33 -0.8531249898354858 1103 34-35 -0.8051416390113744 1103 36-37 -0.7924389553858688 1103 38-39 -0.8330056532919485 1103 40-41 -0.9248513961957769 1103 42-43 -0.9504369790008838 1103 44-45 -0.9136320123291242 1103 46-47 -0.9092126549907391 1103 48-49 -0.902709744456935 1103 50-51 -0.9448545784305367 1103 52-53 -0.943854334577857 1103 54-55 -0.9334411966103104 1103 56-57 -1.0207325419162459 1103 58-59 -1.1046943928352775 1103 60-61 -1.059655083059269 1103 62-63 -1.0792391894703073 1103 64-65 -1.0597375715341215 1103 66-67 -1.0613039079062432 1103 68-69 -1.0960873491483127 1103 70-71 -1.0150153862934204 1103 72-73 -1.10376461052563 1103 74-75 -1.1529120760276754 1103 76-77 -1.0246259974854155 1103 78-79 -0.8889281940004885 1103 80-81 -0.8488182016043915 1103 82-83 -0.8564410897727903 1103 84-85 -0.9189473558326569 1103 86-87 -0.9250809733235847 1103 88-89 -0.9193985807472806 1103 90-91 -1.044028320446433 1103 92-93 -1.026563181874831 1103 94-95 -1.0527886865815503 1103 96-97 -1.1611586614753513 1103 98-99 -1.2995317146934582 1103 100 -1.3056207716794077 1104 1 -0.45931478915520074 1104 2 -0.40024326183079495 1104 3 -0.45498838794823016 1104 4 -0.26046114850984736 1104 5 -0.25799400878639744 1104 6 -0.4232959109371919 1104 7 -0.37169900160822067 1104 8 -0.39213603912880757 1104 9 -0.5383484792618844 1104 10-11 -0.5272376690832701 1104 12-13 -0.6092154102550822 1104 14-15 -0.7669314305679862 1104 16-17 -0.7598219074203101 1104 18-19 -0.8173319584850347 1104 20-21 -0.7411623534937277 1104 22-23 -0.8154420005197309 1104 24-25 -0.9111380259421011 1104 26-27 -0.9343399399465468 1104 28-29 -0.9710032246575722 1104 30-31 -0.8711210656171744 1104 32-33 -0.8474950555910183 1104 34-35 -0.9065850189608895 1104 36-37 -0.8613779809340585 1104 38-39 -0.9848947190618773 1104 40-41 -1.020787917717385 1104 42-43 -0.9687621267090165 1104 44-45 -0.9688952637151118 1104 46-47 -0.9607864343283978 1104 48-49 -0.9808460893914202 1104 50-51 -1.0150428631377153 1104 52-53 -1.001460362221131 1104 54-55 -0.9672178707802104 1104 56-57 -1.0105193912426955 1104 58-59 -1.073248487879475 1104 60-61 -1.117622004893832 1104 62-63 -1.0986101981531888 1104 64-65 -1.0468044575550053 1104 66-67 -1.1266130247154678 1104 68-69 -1.0352871065683544 1104 70-71 -1.0418904700185863 1104 72-73 -1.0420787007375019 1104 74-75 -1.0683696957216071 1104 76-77 -0.9536909246204104 1104 78-79 -0.9265799605702618 1104 80-81 -1.0135919424769924 1104 82-83 -1.0120548208708335 1104 84-85 -1.0012859532845795 1104 86-87 -0.9192622877159025 1104 88-89 -0.9869427921379064 1104 90-91 -0.9446250796734823 1104 92-93 -1.0703828288254975 1104 94-95 -1.1099573856607954 1104 96-97 -1.1591583283447306 1104 98-99 -1.1251512198988465 1104 100 -1.1961917078233242 1105 1 -0.4340892485025094 1105 2 -0.42024560684973267 1105 3 -0.44523808781973173 1105 4 -0.2999555631098758 1105 5 -0.33939024658189254 1105 6 -0.39408014820973136 1105 7 -0.40557663011205847 1105 8 -0.39263213049989787 1105 9 -0.5376750459522128 1105 10-11 -0.5478100769939971 1105 12-13 -0.5831244122957244 1105 14-15 -0.7216076633242423 1105 16-17 -0.7553267407896698 1105 18-19 -0.7235434468348174 1105 20-21 -0.8520555517173918 1105 22-23 -0.7901680866977685 1105 24-25 -0.8312336367332378 1105 26-27 -0.8726825621625522 1105 28-29 -0.8427658754404632 1105 30-31 -0.8350238519948832 1105 32-33 -0.8176557851923505 1105 34-35 -0.8666097531150072 1105 36-37 -0.9647858446243092 1105 38-39 -0.9413308131838463 1105 40-41 -0.9201200198614998 1105 42-43 -0.8996416959605966 1105 44-45 -0.9762308798273835 1105 46-47 -1.114358608859689 1105 48-49 -1.0335916158162775 1105 50-51 -1.07615626014465 1105 52-53 -1.104509626080862 1105 54-55 -1.119217493621882 1105 56-57 -1.1435128657783622 1105 58-59 -1.1069881948308975 1105 60-61 -1.1392270941243368 1105 62-63 -1.1119297830386117 1105 64-65 -1.0923089846990237 1105 66-67 -1.1089405043556937 1105 68-69 -1.1379977756333446 1105 70-71 -1.1774087305706402 1105 72-73 -1.155565123257766 1105 74-75 -1.1822637583923594 1105 76-77 -1.17799399061078 1105 78-79 -1.0666951946351695 1105 80-81 -0.9108206607147658 1105 82-83 -0.9911336765260046 1105 84-85 -0.9276571077468638 1105 86-87 -0.9546367858268816 1105 88-89 -1.0025399821206165 1105 90-91 -1.0230113974785873 1105 92-93 -1.0836841650837918 1105 94-95 -1.1017265355034063 1105 96-97 -1.1721157594783307 1105 98-99 -1.1367953885730486 1105 100 -1.1618364879427325 1106 1 -0.4694427049593841 1106 2 -0.4054537938761875 1106 3 -0.4239241301838632 1106 4 -0.25713544634518115 1106 5 -0.312037955082765 1106 6 -0.40650964948971335 1106 7 -0.4299433583931531 1106 8 -0.44108202785246675 1106 9 -0.5645003607294754 1106 10-11 -0.5822293959027434 1106 12-13 -0.6915625299638535 1106 14-15 -0.8173374852896202 1106 16-17 -0.8319230934171387 1106 18-19 -0.8065991316149663 1106 20-21 -0.7778668741650492 1106 22-23 -0.7839056555538306 1106 24-25 -0.8668469807646417 1106 26-27 -0.9203771864081958 1106 28-29 -0.8810304861153853 1106 30-31 -0.8641303356687828 1106 32-33 -0.9029942815239593 1106 34-35 -0.8960741288489515 1106 36-37 -0.9635793200950715 1106 38-39 -0.9740077866138677 1106 40-41 -0.9822540151535364 1106 42-43 -0.9417136432845936 1106 44-45 -1.1009837037903552 1106 46-47 -1.1011272091841917 1106 48-49 -1.1211799400607632 1106 50-51 -1.116473145013174 1106 52-53 -1.185400476737179 1106 54-55 -1.1352286351294865 1106 56-57 -1.1546962230001725 1106 58-59 -1.1518222934383147 1106 60-61 -1.251723156648083 1106 62-63 -1.1960174140392539 1106 64-65 -1.2690322090228747 1106 66-67 -1.2211456646764134 1106 68-69 -1.2126026758097552 1106 70-71 -1.1344294612226378 1106 72-73 -1.232215727008473 1106 74-75 -1.229484231954732 1106 76-77 -1.149590454416611 1106 78-79 -1.0628875969018843 1106 80-81 -0.9985907104482621 1106 82-83 -0.99040834107522 1106 84-85 -0.9446173012381109 1106 86-87 -1.0045193719060173 1106 88-89 -1.0884229695732124 1106 90-91 -1.1043775301830827 1106 92-93 -1.1150799003863447 1106 94-95 -1.0406038934147404 1106 96-97 -1.0844013750742079 1106 98-99 -1.1236733070321847 1106 100 -1.2069707898781807 1107 1 -0.46802181283986144 1107 2 -0.4024597986508489 1107 3 -0.4600753498341845 1107 4 -0.3055247453320149 1107 5 -0.33682496326863287 1107 6 -0.4146009403776816 1107 7 -0.42268771179904263 1107 8 -0.46251450064848854 1107 9 -0.6365950260547208 1107 10-11 -0.5575713228929757 1107 12-13 -0.5986115322067462 1107 14-15 -0.8084404990287126 1107 16-17 -0.8090585497065064 1107 18-19 -0.8303475368482722 1107 20-21 -0.8233904831609848 1107 22-23 -0.853216021485018 1107 24-25 -0.8422069945541679 1107 26-27 -0.8286990444823843 1107 28-29 -0.8760192468886174 1107 30-31 -0.9290841425812957 1107 32-33 -0.9051502301355825 1107 34-35 -0.9304979630735914 1107 36-37 -0.9228341708700114 1107 38-39 -0.9149531910870152 1107 40-41 -0.9230238047064745 1107 42-43 -0.9358788567790839 1107 44-45 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0.8159835342047153 2116 96-97 0.8902004689148058 2116 98-99 0.9998229252386466 2116 100 0.64764751308158 2201 1 0.7424995094495692 2201 2 0.6812829789477775 2201 3 0.6601712709195198 2201 4 0.4388449639938443 2201 5 0.5283541629343844 2201 6 0.532516961285495 2201 7 0.5830159649814988 2201 8 0.6076314427742204 2201 9 0.7507170871225597 2201 10-11 0.681563610091672 2201 12-13 0.7070396130823937 2201 14-15 0.9085120646124594 2201 16-17 0.8471925646588829 2201 18-19 0.8802025093587034 2201 20-21 0.8649775615087592 2201 22-23 0.8568817806518965 2201 24-25 0.8391306786361596 2201 26-27 0.8622200606288644 2201 28-29 0.9199950415789573 2201 30-31 1.0331310501432327 2201 32-33 0.8605946889361817 2201 34-35 1.0237751277509446 2201 36-37 1.0591185917368264 2201 38-39 1.1138304511883987 2201 40-41 1.0985743919639503 2201 42-43 0.1851960899468068 2201 44-45 0.9134235354585982 2201 46-47 1.1084228911445564 2201 48-49 1.1147918216603188 2201 50-51 1.1147081491974546 2201 52-53 1.0994580689367623 2201 54-55 1.1497896906324954 2201 56-57 1.1540061834134434 2201 58-59 1.1089264897302442 2201 60-61 1.189334828320952 2201 62-63 1.197245058087553 2201 64-65 1.1931826913464718 2201 66-67 1.1652208668734687 2201 68-69 1.1544701157169257 2201 70-71 1.2216846237638492 2201 72-73 1.198173953619758 2201 74-75 1.1756400706616859 2201 76-77 1.1868561095399244 2201 78-79 0.998335577232293 2201 80-81 0.9531288825450481 2201 82-83 0.941150646478274 2201 84-85 0.9230954894224475 2201 86-87 0.8904795904285514 2201 88-89 0.9119179274224614 2201 90-91 0.3248372896195697 2201 92-93 0.7115650488896712 2201 94-95 0.8573055763227586 2201 96-97 0.8957575796620638 2201 98-99 0.9022723183151982 2201 100 1.063055168630104 2202 1 0.7381944754642582 2202 2 0.6906974154035339 2202 3 0.6603258076250924 2202 4 0.45821770397432005 2202 5 0.4583116052782046 2202 6 0.511118879374429 2202 7 0.5847383267619648 2202 8 0.6130195103817968 2202 9 0.7684716891014673 2202 10-11 0.7851995343801761 2202 12-13 0.7907266070225631 2202 14-15 0.9546888276772236 2202 16-17 0.9519468634835562 2202 18-19 0.9246696194445505 2202 20-21 0.9056076000553119 2202 22-23 0.9343161893084542 2202 24-25 0.9017251822129637 2202 26-27 0.9675398721068476 2202 28-29 0.9680937062146953 2202 30-31 1.09226611928608 2202 32-33 0.6838687060177406 2202 34-35 1.0611813089406255 2202 36-37 1.1497830659127786 2202 38-39 1.169714388377436 2202 40-41 1.1562798950259037 2202 42-43 1.2130672171281347 2202 44-45 1.20093672591792 2202 46-47 1.2498021860811406 2202 48-49 1.2336000819402884 2202 50-51 1.201003465069789 2202 52-53 1.193635308088325 2202 54-55 1.2118347271433194 2202 56-57 1.1670497951457364 2202 58-59 1.190122123334227 2202 60-61 1.1858867389587004 2202 62-63 1.2394358709355302 2202 64-65 1.2242587287444806 2202 66-67 1.258252543938525 2202 68-69 1.2292092719175045 2202 70-71 1.2185361777659196 2202 72-73 1.2439715417561672 2202 74-75 1.2508088307794054 2202 76-77 1.235939271062641 2202 78-79 1.0760376251250925 2202 80-81 1.0452060937952758 2202 82-83 1.0222315816975076 2202 84-85 0.9702202188236306 2202 86-87 0.996830700841226 2202 88-89 1.0535325092808385 2202 90-91 1.1097218762922019 2202 92-93 1.053751996869238 2202 94-95 1.0507423856080997 2202 96-97 0.9342619695954859 2202 98-99 0.9537475200195473 2202 100 1.1351419283104995 2203 1 0.6941538170427641 2203 2 0.6584921521448805 2203 3 0.6316454318222142 2203 4 0.396968857049508 2203 5 0.5167088113976845 2203 6 0.5329552567343683 2203 7 0.5406691886587041 2203 8 0.553079678295056 2203 9 0.7184047279174237 2203 10-11 0.7414185799810937 2203 12-13 0.7365545334534715 2203 14-15 0.9188622032226519 2203 16-17 0.8961675966952356 2203 18-19 0.8674063227675219 2203 20-21 0.9223985690996912 2203 22-23 0.8940797576927721 2203 24-25 0.884018495276969 2203 26-27 0.9179856397416586 2203 28-29 0.9204026217741301 2203 30-31 1.0024247234427293 2203 32-33 1.050497177624763 2203 34-35 1.0726234302685143 2203 36-37 1.1025874659540662 2203 38-39 1.1297835896549557 2203 40-41 1.1238168649094504 2203 42-43 1.1665381709370664 2203 44-45 1.1696503300191878 2203 46-47 1.1768645119285708 2203 48-49 1.162259363659146 2203 50-51 1.0963003155056725 2203 52-53 1.134044042724149 2203 54-55 1.1217158986493914 2203 56-57 1.152752157632726 2203 58-59 1.1578388177635546 2203 60-61 1.1765781691558317 2203 62-63 1.1920020890215497 2203 64-65 1.177855084548689 2203 66-67 1.1853847936553024 2203 68-69 1.1725505712555915 2203 70-71 1.1713060481628048 2203 72-73 1.2080395918536482 2203 74-75 1.2042733280732492 2203 76-77 1.1854376086644294 2203 78-79 1.0162751497050202 2203 80-81 0.9499960289652272 2203 82-83 0.9444000760587024 2203 84-85 0.9941478357889189 2203 86-87 0.9748546553820567 2203 88-89 0.9891904096374269 2203 90-91 1.0465816622907234 2203 92-93 1.0627298168216441 2203 94-95 1.0650815608052504 2203 96-97 0.9950213578390645 2203 98-99 1.0277582257998077 2203 100 1.2914511541776577 2204 1 0.7444704359747156 2204 2 0.7153659417173657 2204 3 0.6787283891311588 2204 4 0.47478502106866216 2204 5 0.5637537438041491 2204 6 0.5576769916519737 2204 7 0.6043677343062157 2204 8 0.6125454642261801 2204 9 0.7909991669929539 2204 10-11 0.7900036348727824 2204 12-13 0.8193327904559098 2204 14-15 0.9964743061186638 2204 16-17 0.9783340727579599 2204 18-19 0.9447432045175503 2204 20-21 0.9505886480048034 2204 22-23 0.972408751983842 2204 24-25 0.9563980724344532 2204 26-27 0.9797797124652661 2204 28-29 1.0106537745303328 2204 30-31 1.107454820354782 2204 32-33 1.161450051869842 2204 34-35 1.1646511136183975 2204 36-37 1.2108323061123585 2204 38-39 1.223675182060738 2204 40-41 1.1953128109832178 2204 42-43 1.288115419098233 2204 44-45 1.280778433651598 2204 46-47 1.2616681850442575 2204 48-49 1.2784721069113232 2204 50-51 1.2790012209214723 2204 52-53 1.2914034944875468 2204 54-55 1.3202695414469545 2204 56-57 1.2911256338473152 2204 58-59 1.3149208898944167 2204 60-61 1.3420496785110672 2204 62-63 1.3222334707099463 2204 64-65 1.3565466529433152 2204 66-67 1.3331136000340678 2204 68-69 1.3717759988283618 2204 70-71 1.3833018608786887 2204 72-73 1.3628071444687038 2204 74-75 1.3710743857011352 2204 76-77 1.3459382907440869 2204 78-79 1.2029310515898821 2204 80-81 1.1801379704970536 2204 82-83 1.1231969211068922 2204 84-85 1.1137465106533675 2204 86-87 1.1579066289660567 2204 88-89 1.1615199147152602 2204 90-91 1.2132427203766092 2204 92-93 1.2165769165584948 2204 94-95 1.2660738899266342 2204 96-97 1.1717286666576427 2204 98-99 1.1543149529523618 2204 100 1.3719651901498402 2205 1 0.7130268985166843 2205 2 0.5148589936308028 2205 3 0.6153203957522919 2205 4 0.4255129305779377 2205 5 0.5518362064529825 2205 6 0.5624434014832858 2205 7 0.6000288332786994 2205 8 0.609215137420513 2205 9 0.7637153544074096 2205 10-11 0.7953499674092868 2205 12-13 0.8022807036888011 2205 14-15 0.9757034555750792 2205 16-17 0.9610414937348821 2205 18-19 0.9742776591948683 2205 20-21 0.9521190902391083 2205 22-23 0.9712639539870693 2205 24-25 0.9945007893635776 2205 26-27 1.0301164026402816 2205 28-29 1.0560245548999632 2205 30-31 1.1293694176806497 2205 32-33 1.1736779953738008 2205 34-35 1.1829871673877435 2205 36-37 1.2054024589561365 2205 38-39 1.2064526338490609 2205 40-41 1.1832740383051288 2205 42-43 1.2728125634104188 2205 44-45 1.2661310071488998 2205 46-47 1.31306606913126 2205 48-49 1.2780946751599203 2205 50-51 1.26145426120803 2205 52-53 1.320035911566741 2205 54-55 1.2975473623132388 2205 56-57 1.2801437495750676 2205 58-59 1.2987518405826677 2205 60-61 1.2859472690001752 2205 62-63 1.3052648985625481 2205 64-65 1.3599007591560834 2205 66-67 1.3658917945857425 2205 68-69 1.4167429816858501 2205 70-71 1.4340709916190946 2205 72-73 1.4426288385240724 2205 74-75 1.418799020345844 2205 76-77 1.3183792856578975 2205 78-79 1.2264424772767732 2205 80-81 1.1656008757667244 2205 82-83 1.1892728145150748 2205 84-85 1.1817983772266167 2205 86-87 1.230600974908679 2205 88-89 1.2775248344722705 2205 90-91 1.327802043311845 2205 92-93 1.3303949248647307 2205 94-95 1.2947647104089768 2205 96-97 1.2978692643713714 2205 98-99 1.3856005534646556 2205 100 1.5440617144780937 2206 1 0.7230947065077693 2206 2 0.7102003547723399 2206 3 0.6821230326723011 2206 4 0.35439010840433127 2206 5 0.5198069373699354 2206 6 0.5540611736567129 2206 7 0.6123592695350055 2206 8 0.6181241029870677 2206 9 0.8079405227287566 2206 10-11 0.7868612217087261 2206 12-13 0.8119398495994616 2206 14-15 1.0079620337365185 2206 16-17 1.0071859066218707 2206 18-19 0.9750030445807099 2206 20-21 0.9898455638302011 2206 22-23 0.9991281213491234 2206 24-25 1.0148605278196001 2206 26-27 1.0427465852509314 2206 28-29 1.086196365425124 2206 30-31 1.1583815851777572 2206 32-33 1.183430094421162 2206 34-35 1.2185786906673286 2206 36-37 1.2903582502927193 2206 38-39 1.254593139343548 2206 40-41 1.2659009640527472 2206 42-43 1.3327606873106745 2206 44-45 1.3454471137283477 2206 46-47 1.3470460309612946 2206 48-49 1.3884150180443342 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0.809038694084208 2207 10-11 0.8224395860950722 2207 12-13 0.8655485089679402 2207 14-15 1.0770040862869834 2207 16-17 1.0321208834396884 2207 18-19 1.0328902876366541 2207 20-21 1.0393718241639007 2207 22-23 1.0495311049845455 2207 24-25 1.0693198604993626 2207 26-27 1.0822301347051067 2207 28-29 1.1160906033893312 2207 30-31 1.1617704437032685 2207 32-33 1.215289279000011 2207 34-35 1.2149673198788875 2207 36-37 1.209081262178735 2207 38-39 1.2628785632125243 2207 40-41 1.293839787015159 2207 42-43 1.3594039512458451 2207 44-45 1.3544082641699617 2207 46-47 1.3558028532363267 2207 48-49 1.3492530911186762 2207 50-51 1.3253726575890497 2207 52-53 1.332901279553802 2207 54-55 1.305314740752813 2207 56-57 1.3310755782224533 2207 58-59 1.249459604799739 2207 60-61 1.2230873326801373 2207 62-63 1.318535224691253 2207 64-65 1.3261027249869528 2207 66-67 1.3696283879411482 2207 68-69 1.4251839300547502 2207 70-71 1.4304726655779305 2207 72-73 1.44433891539407 2207 74-75 1.4067480548660356 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1.336951212829554 2311 60-61 1.3566196675816542 2311 62-63 1.3434872607222204 2311 64-65 1.3732814281252317 2311 66-67 1.394975795111904 2311 68-69 1.4061037989590872 2311 70-71 1.4181810490923823 2311 72-73 1.4094246393831824 2311 74-75 1.2802963147361908 2311 76-77 1.1712728994972963 2311 78-79 1.1447152199088286 2311 80-81 1.1434260004870502 2311 82-83 1.1646751142945 2311 84-85 1.1958551784931153 2311 86-87 1.2362740601247388 2311 88-89 1.272814429406747 2311 90-91 1.3121368590076976 2311 92-93 1.3388050916857601 2311 94-95 1.380048358833509 2311 96-97 1.452580056027383 2311 98-99 1.418413329487148 2311 100 1.5618170264433964 2312 1 0.2143314293988574 2312 2 0.21834475327713676 2312 3 0.38175223816342907 2312 4 0.2995924808754822 2312 5 0.367412512721927 2312 6 0.48724747310441074 2312 7 0.5193767219280545 2312 8 0.33944955162471757 2312 9 0.5014616100839788 2312 10-11 0.39308351236436323 2312 12-13 0.5460353369515758 2312 14-15 0.9403204603415745 2312 16-17 0.961895252777154 2312 18-19 1.0079035906163014 2312 20-21 0.9849536241792478 2312 22-23 0.6171160596800931 2312 24-25 0.5858840236151437 2312 26-27 0.9124738310094429 2312 28-29 1.0643221044062656 2312 30-31 0.9716113779993876 2312 32-33 0.839099686043852 2312 34-35 0.9950161076822539 2312 36-37 1.0768668864539777 2312 38-39 1.0671973934371692 2312 40-41 1.0859569041694783 2312 42-43 0.9580406582267713 2312 44-45 0.8885197489633825 2312 46-47 1.053484006070569 2312 48-49 1.1119942967690761 2312 50-51 1.1365919014607186 2312 52-53 1.1795468240216351 2312 54-55 1.1486781121540162 2312 56-57 1.1670354895929123 2312 58-59 1.1967170961624234 2312 60-61 1.1745675020600999 2312 62-63 1.1986924843657718 2312 64-65 1.257395698947576 2312 66-67 1.23531129516347 2312 68-69 1.282758797317726 2312 70-71 1.1415487904902903 2312 72-73 1.2550110789713926 2312 74-75 1.293658480520719 2312 76-77 1.2122043253621655 2312 78-79 1.0851491042734942 2312 80-81 0.9774827452802661 2312 82-83 0.9857152247312335 2312 84-85 0.8304095091077848 2312 86-87 1.0125374211352174 2312 88-89 0.6738090219186468 2312 90-91 1.0478525068617621 2312 92-93 1.2413994302073377 2312 94-95 1.2990907509282543 2312 96-97 1.3386016416585491 2312 98-99 1.3594843347906291 2312 100 1.4878864140212151 2313 1 -0.2969907942958159 2313 2 0.04292444183353439 2313 3 0.3098398951411241 2313 4 0.1689815551760745 2313 5 0.305880772111756 2313 6 0.4123695902281739 2313 7 0.2013776739689277 2313 8 0.17346526408804408 2313 9 0.35347992379428916 2313 10-11 0.44717158852837713 2313 12-13 0.6510957433072377 2313 14-15 0.7353586554136626 2313 16-17 0.8807551159094942 2313 18-19 0.7590255216198543 2313 20-21 0.9661565581322691 2313 22-23 1.0068147039043964 2313 24-25 0.8408227240858182 2313 26-27 1.0459048728278972 2313 28-29 0.9346789702548151 2313 30-31 0.9496327504482736 2313 32-33 1.1161403799228822 2313 34-35 1.1409805175998926 2313 36-37 0.9853844351215457 2313 38-39 0.7315056923558245 2313 40-41 0.8929757035111976 2313 42-43 0.9307399886653513 2313 44-45 1.144682549015208 2313 46-47 1.1909922320012711 2313 48-49 1.2230596209860352 2313 50-51 1.188596065553682 2313 52-53 1.1438378058602225 2313 54-55 1.1972018330997614 2313 56-57 1.2042529165707663 2313 58-59 1.147793012319248 2313 60-61 1.1900730926433383 2313 62-63 1.2092664036579919 2313 64-65 1.3435044839412242 2313 66-67 1.354233365091801 2313 68-69 1.362823492801418 2313 70-71 1.2880194399629303 2313 72-73 1.3562813135364493 2313 74-75 0.9862301982530823 2313 76-77 1.1356207372456169 2313 78-79 1.0095820441206307 2313 80-81 1.1079576156337758 2313 82-83 1.0979640906825487 2313 84-85 1.1136210198844587 2313 86-87 1.2091490111469732 2313 88-89 1.215366716085267 2313 90-91 1.3176009495605854 2313 92-93 1.0367969567123687 2313 94-95 1.0670905566756907 2313 96-97 1.3409023897711947 2313 98-99 1.4404074912210518 2313 100 1.4451165184740162 2314 1 -0.2668137865760336 2314 2 -0.07760468669340881 2314 3 0.20333050405572806 2314 4 -0.11165302073239758 2314 5 0.27114780319385545 2314 6 0.3056991192312353 2314 7 0.22228506910969514 2314 8 0.2589120405855425 2314 9 0.3607300866217926 2314 10-11 0.527797402364591 2314 12-13 0.6187128808840683 2314 14-15 0.6896081414403881 2314 16-17 0.8719531503127484 2314 18-19 0.9035994275901729 2314 20-21 0.5201970756658127 2314 22-23 0.7643517131138466 2314 24-25 0.9826270709896434 2314 26-27 0.9556069575502661 2314 28-29 0.9049443824024479 2314 30-31 0.873597510707377 2314 32-33 0.9923604261702295 2314 34-35 1.0397352397180626 2314 36-37 0.9805379741671274 2314 38-39 0.9901837068702477 2314 40-41 1.0248219161265553 2314 42-43 0.8209032925402866 2314 44-45 0.8922342081804757 2314 46-47 1.0099177943407085 2314 48-49 0.9137796373435094 2314 50-51 0.9905029748031922 2314 52-53 1.0882561067075969 2314 54-55 1.1529061304562944 2314 56-57 1.0051709289446933 2314 58-59 0.9892699204623625 2314 60-61 1.0538203154762158 2314 62-63 0.8073607553875846 2314 64-65 0.9541603795865115 2314 66-67 1.1045738821794089 2314 68-69 1.1830695277528065 2314 70-71 1.2321550072992764 2314 72-73 1.2390266705420814 2314 74-75 1.2401634738073568 2314 76-77 1.0049387985401097 2314 78-79 0.9983147890452635 2314 80-81 1.0155486827312394 2314 82-83 0.9853244654943545 2314 84-85 0.9064234340101187 2314 86-87 1.048442278609297 2314 88-89 1.099722691420812 2314 90-91 1.2031053800716869 2314 92-93 1.2220650990228883 2314 94-95 1.2813724271165832 2314 96-97 1.383800740754019 2314 98-99 1.351847670673596 2314 100 1.3986002798351826 2315 1 -0.0016719669517790692 2315 2 0.19344465292959256 2315 3 0.17321728413236315 2315 4 0.002338967110965484 2315 5 0.2852074690966546 2315 6 0.4090475985345208 2315 7 0.4881079199923235 2315 8 0.45085099103653903 2315 9 0.6328655039384827 2315 10-11 0.6271916152007933 2315 12-13 0.6914203880236371 2315 14-15 0.8085293211498552 2315 16-17 0.7923297081957941 2315 18-19 0.8830916695483566 2315 20-21 0.8924446422377414 2315 22-23 0.9816339740606921 2315 24-25 0.9203841554076462 2315 26-27 1.0277374994507582 2315 28-29 1.0404898558740072 2315 30-31 0.9984756197964728 2315 32-33 1.0142103350474443 2315 34-35 1.0357549663817167 2315 36-37 1.0905295879520551 2315 38-39 1.1027181409195137 2315 40-41 1.0460449731935526 2315 42-43 1.089469031210399 2315 44-45 1.038213406341093 2315 46-47 1.017565696966173 2315 48-49 1.122819706583961 2315 50-51 1.0133168704103497 2315 52-53 1.0428425757560191 2315 54-55 0.9561487183040143 2315 56-57 1.0870568402120355 2315 58-59 1.1083249667229467 2315 60-61 1.1650175119144208 2315 62-63 1.155755405124495 2315 64-65 1.1965951281496743 2315 66-67 1.2012244437236888 2315 68-69 1.1256887955121329 2315 70-71 0.7662494108911702 2315 72-73 1.031396131401948 2315 74-75 1.1621695777021586 2315 76-77 1.0868486133688577 2315 78-79 0.9977253471161838 2315 80-81 1.0209900722705179 2315 82-83 1.0008168584502322 2315 84-85 0.903045708816915 2315 86-87 0.875928134102633 2315 88-89 1.0493312786783413 2315 90-91 1.1002749990826892 2315 92-93 1.133474137837485 2315 94-95 1.1963359662567399 2315 96-97 1.2528856499482366 2315 98-99 1.173748788673116 2315 100 0.9793209291909903 2316 1 0.5580485192456699 2316 2 0.5398398412291598 2316 3 0.5311914832574516 2316 4 0.3554677963437811 2316 5 0.4493652635982812 2316 6 0.4767163974333073 2316 7 0.5320679095449847 2316 8 0.5318277657151143 2316 9 0.5805953657495664 2316 10-11 0.5784422585745901 2316 12-13 0.7146547596950299 2316 14-15 0.8797079670288426 2316 16-17 0.8654939945382267 2316 18-19 0.8844966954571802 2316 20-21 0.9389975555939216 2316 22-23 0.9683012826818711 2316 24-25 1.0273970932999035 2316 26-27 1.0667572283148559 2316 28-29 1.0164080585870963 2316 30-31 0.8976834682691006 2316 32-33 0.8855629134441116 2316 34-35 0.8193181827490648 2316 36-37 0.8115588835594068 2316 38-39 0.7452665659185556 2316 40-41 0.8635597276506246 2316 42-43 1.031100369240491 2316 44-45 0.9492738729916042 2316 46-47 0.9766210938405351 2316 48-49 0.7150857319928363 2316 50-51 0.7948208466992668 2316 52-53 0.9616501624132496 2316 54-55 1.0288773426138746 2316 56-57 1.1060557742723347 2316 58-59 1.0341542947437645 2316 60-61 1.0454908674731485 2316 62-63 1.0956014655533082 2316 64-65 1.0836533545279963 2316 66-67 1.070203434989807 2316 68-69 1.042184334905464 2316 70-71 0.9366154101933049 2316 72-73 0.9657825230717663 2316 74-75 1.0428792054007658 2316 76-77 0.9023944544653517 2316 78-79 0.8563691376771452 2316 80-81 0.8467060861276927 2316 82-83 0.7559329222219979 2316 84-85 0.832964889587199 2316 86-87 0.827026014020614 2316 88-89 0.7597101187859536 2316 90-91 0.8325850115539488 2316 92-93 0.8763663439366702 2316 94-95 0.7363171828363235 2316 96-97 0.9249579514443802 2316 98-99 1.02206713902536 2316 100 0.6885080230106588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 3.0 11 41.0 12 110.0 13 659.0 14 2458.0 15 6770.0 16 16548.0 17 33484.0 18 55856.0 19 79490.0 20 110096.0 21 149093.0 22 172934.0 23 171088.0 24 173229.0 25 193846.0 26 232492.0 27 285986.0 28 351883.0 29 428751.0 30 522857.0 31 650822.0 32 817056.0 33 1064273.0 34 1476181.0 35 1996590.0 36 2954095.0 37 3973073.0 38 2935172.0 39 395895.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.56302801351877 21.918805798191507 13.803501805483812 23.714664382805914 2 15.212369616775822 21.34755940868472 40.27623304055141 23.163837933988045 3 16.978104911435846 28.747545497608495 30.86115639483291 23.413193196122748 4 11.699372032658314 18.123857880468254 40.366840493884524 29.809929592988905 5 12.362469216969469 39.23546915764496 35.129849505876706 13.272212119508858 6 28.21045744117129 39.584860863524604 18.930694322177096 13.27398737312701 7 25.6802638310724 33.236868093242656 24.28721025299568 16.795657822689268 8 26.298493936584293 34.58469715231843 20.703952665198084 18.412856245899192 9 27.38523704984938 14.191095735602902 21.447914496271764 36.97575271827595 10-11 24.843246548143657 26.32856973846525 28.426226744830092 20.401956968561002 12-13 25.482622414005412 24.473643016043575 28.399056049103855 21.644678520847158 14-15 23.0774858895122 25.301632933897096 26.24733190220967 25.373549274381034 16-17 21.614582893687775 27.723428748875474 27.41282914588174 23.249159211555014 18-19 21.229919326151244 27.542781492015312 29.71957391175703 21.507725270076413 20-21 22.787971355234397 26.467830220665096 28.739525071286476 22.004673352814034 22-23 22.99113844208677 26.296470843686233 28.428340622406363 22.284050091820635 24-25 22.283233457099453 26.644926820282933 29.185958046683297 21.88588167593432 26-27 22.144980018021027 26.781794420878956 28.654670553179844 22.418555007920173 28-29 21.604085007247534 27.31717952106439 28.76514335487646 22.313592116811616 30-31 22.541958416933124 26.714636013299568 28.57087015007483 22.172535419692476 32-33 22.721473017283493 27.174243311180028 27.85237876157844 22.251904909958036 34-35 22.633483871369993 26.918770250414102 28.901731325974772 21.546014552241132 36-37 22.494285792079527 26.734883303788017 28.67969892666214 22.09113197747032 38-39 22.53460806389236 26.47614792656859 29.14494108963656 21.844302919902486 40-41 22.147014739841183 25.97922592172447 29.595667051654633 22.27809228677971 42-43 22.12567095624999 26.23516208142018 28.82979983387453 22.8093671284553 44-45 21.862611799660495 27.099462605233143 28.73828702378971 22.299638571316656 46-47 22.04585611678984 26.52586079253958 28.38332575688699 23.044957333783593 48-49 22.516591158762132 26.261213003044542 28.495036925201696 22.727158912991634 50-51 22.243028257897638 26.471369484733064 27.774814269138247 23.510787988231048 52-53 21.497458898748405 26.961143506473466 28.000828036060355 23.54056955871777 54-55 21.664179182403604 26.84349390434314 27.854100515075224 23.638226398178027 56-57 22.354972771214175 26.368183988749273 27.687825246427884 23.589017993608675 58-59 21.60839677614125 26.51097955937861 28.364408717878852 23.516214946601284 60-61 21.910128381273697 27.258334294777775 27.346378537048476 23.48515878690005 62-63 22.007755461868875 26.615195267536112 27.153001936398024 24.224047334196992 64-65 21.14223342588146 26.500057176901283 27.562511030777692 24.795198366439564 66-67 21.71291274330425 26.170556799370885 27.435014712625303 24.68151574469956 68-69 21.28445233901396 26.364283506704073 27.554672857839563 24.796591296442404 70-71 21.369245886533935 25.919563534294355 27.7128438780454 24.99834670112631 72-73 21.654094578115 26.185923830380553 27.577123120688984 24.58285847081546 74-75 21.417088805585895 26.449963824086936 27.42742922704613 24.70551814328104 76-77 21.04378466802032 26.23860366264984 27.426775386496345 25.2908362828335 78-79 20.79095023183916 26.247931568359984 27.599085678079703 25.362032521721144 80-81 20.59582846105058 26.517771108331438 27.687368313304084 25.19903211731389 82-83 21.13408752321554 25.609834036935364 28.264365993325896 24.9917124465232 84-85 21.379927980122556 25.92004639872029 27.209507787948283 25.490517833208866 86-87 20.25925939880933 26.463025094024147 27.667493038893458 25.610222468273065 88-89 20.798126885108378 25.586100757759645 27.563300399540324 26.052471957591656 90-91 20.730550691363135 25.281431616400283 27.87600562512481 26.112012067111774 92-93 20.887962471685924 25.563364673868893 27.441058568768383 26.1076142856768 94-95 19.851807687196672 25.27282766723357 28.103514717306627 26.771849928263126 96-97 20.020651795175024 25.160533227221567 27.93309192386498 26.88572305373843 98-99 19.666691571475052 25.695459846503944 27.809909252628174 26.82793932939283 100 19.994997742281793 25.211950408236287 27.528123541841616 27.264928307640307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 986.0 1 724.0 2 855.0 3 1269.0 4 1052.0 5 1003.5 6 1552.0 7 3051.0 8 4983.5 9 6032.0 10 5973.0 11 6069.0 12 7207.5 13 8976.5 14 11153.5 15 13646.0 16 16090.0 17 18344.5 18 20957.0 19 23529.5 20 25852.0 21 28398.5 22 31290.5 23 35238.5 24 40006.0 25 45946.5 26 56641.0 27 68104.5 28 83692.5 29 119638.0 30 168779.0 31 213211.0 32 281532.0 33 350942.0 34 407724.0 35 468756.5 36 528497.0 37 619624.5 38 650258.5 39 603299.0 40 560617.0 41 532308.0 42 537817.0 43 567099.5 44 611635.0 45 698377.0 46 768939.5 47 819243.5 48 867727.5 49 858871.5 50 826573.5 51 811381.0 52 790897.0 53 759581.5 54 727018.0 55 652832.0 56 571791.5 57 480022.0 58 387823.0 59 344260.5 60 284952.5 61 212586.0 62 163561.0 63 128546.0 64 92609.5 65 59290.0 66 39544.5 67 27892.0 68 20745.0 69 15487.5 70 12040.0 71 9159.5 72 7137.5 73 6094.0 74 4966.0 75 3715.0 76 2538.0 77 1611.0 78 1062.0 79 690.0 80 464.5 81 321.0 82 213.0 83 151.5 84 98.5 85 55.5 86 35.0 87 24.5 88 17.0 89 12.0 90 5.5 91 2.5 92 2.0 93 1.5 94 1.5 95 2.0 96 1.5 97 1.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05062114666325348 2 5.194576363597074E-6 3 5.194576363597074E-6 4 0.01657589317623826 5 0.004124493632696076 6 5.194576363597074E-6 7 2.3375593636186835E-4 8 3.376474636338098E-4 9 0.0016570698599874664 10-11 8.07756624539345E-4 12-13 3.038827172704288E-4 14-15 3.895932272697805E-5 16-17 9.609966272654586E-5 18-19 0.0 20-21 5.194576363597074E-6 22-23 2.597288181798537E-6 24-25 1.1428067999913562E-4 26-27 1.0389152727194147E-5 28-29 8.311322181755318E-4 30-31 7.869783190849566E-4 32-33 0.013653943971714908 34-35 0.022482126501648134 36-37 0.030315547657952524 38-39 0.020012105440757724 40-41 1.558372909079122E-4 42-43 0.001755766810895811 44-45 0.008747666596297473 46-47 0.013495509392625197 48-49 0.005127046870870312 50-51 0.009833333056289261 52-53 0.015033103996249932 54-55 0.009428156099928689 56-57 0.0028076685245242185 58-59 0.00808016353357525 60-61 0.001553178332715525 62-63 8.155484890847406E-4 64-65 0.01851866473622357 66-67 0.04431493095784664 68-69 0.053693738582321154 70-71 0.04683170320600941 72-73 0.03926580273243028 74-75 0.02757021404979147 76-77 0.02781955371524413 78-79 0.011773507328092769 80-81 0.0019453688481671043 82-83 0.02659623098161702 84-85 0.046429123537830644 86-87 0.04735116084236913 88-89 0.058376649174103915 90-91 0.033634881954291054 92-93 0.025450826893443867 94-95 0.006929564869038497 96-97 0.0036647736245177358 98-99 0.02197305801801562 100 0.018383605750770044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.9250848E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.31506081504818 #Duplication Level Percentage of deduplicated Percentage of total 1 68.78087638100439 33.23152225260537 2 15.481726575596221 14.96001122043758 3 6.368917505158418 9.231439097632617 4 3.194066233809809 6.172860173352514 5 1.8314959344321833 4.424441872730221 6 1.1349130870966646 3.2900036893721647 7 0.7224077749315433 2.4432222905356804 8 0.4938957931883256 1.9090084227352329 9 0.35913550813068823 1.5616488524559688 >10 1.5150667512148825 12.291639384116761 >50 0.07298945905055809 2.4105983153221726 >100 0.0388210207322552 3.653129313294622 >500 0.0036185510095457173 1.2088588557276307 >1k 0.0019279273353920705 1.6510595674977122 >5k 7.074865458992295E-5 0.23506282544738794 >10k+ 7.074865458992295E-5 1.325493866736427 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58609 0.3044489260940609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45800 0.23791159745274598 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32969 0.17125998813143192 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.194576363597074E-6 0.0 5 0.0 0.0 0.0 1.0389152727194147E-5 0.0 6 0.0 0.0 0.0 1.0389152727194147E-5 1.5583729090791223E-5 7 0.0 0.0 0.0 1.0389152727194147E-5 1.5583729090791223E-5 8 0.0 0.0 0.0 1.0389152727194147E-5 1.5583729090791223E-5 9 0.0 0.0 0.0 1.0389152727194147E-5 3.1167458181582445E-5 10-11 0.0 0.0 0.0 2.0778305454388295E-5 3.1167458181582445E-5 12-13 0.0 0.0 0.0 3.636203454517952E-5 3.376474636338098E-5 14-15 0.0 0.0 0.0 4.155661090877659E-5 8.051593363575464E-5 16-17 0.0 0.0 0.0 8.830779818115025E-5 1.0389152727194148E-4 18-19 0.0 0.0 0.0 1.40253561817121E-4 1.0908610363553855E-4 20-21 0.0 0.0 0.0 3.5582848090639954E-4 1.1168339181733709E-4 22-23 0.0 0.0 0.0 0.0011064447654461768 1.4285084999891953E-4 24-25 0.0 0.0 0.0 0.002776501066342636 1.7142101999870343E-4 26-27 0.0 0.0 0.0 0.005438721452686136 1.8181017272589757E-4 28-29 0.0 0.0 0.0 0.0166849792798738 1.8700474908949464E-4 30-31 0.0 0.0 0.0 0.06005449733954577 1.9219932545309172E-4 32-33 0.0 0.0 0.0 0.14954406164341436 2.0778305454388296E-4 34-35 0.0 0.0 0.0 0.2735489885952037 2.2856135999827125E-4 36-37 0.0 0.0 0.0 0.4779763468082029 2.3115864818006978E-4 38-39 0.0 0.0 0.0 0.815465375862923 2.441450890890625E-4 40-41 0.0 0.0 0.0 1.1982277352145734 2.519369536344581E-4 42-43 0.0 0.0 0.0 1.5735774340953705 2.597288181798537E-4 44-45 0.0 0.0 0.0 1.9899097431967674 2.7011797090704785E-4 46-47 0.0 0.0 0.0 2.481685482114866 2.8310441181604053E-4 48-49 0.0 0.0 0.0 3.0258770938298403 3.2206373454301857E-4 50-51 0.0 0.0 0.0 3.607734578757258 3.2206373454301857E-4 52-53 0.0 0.0 0.0 4.168174825337564 3.610230572699966E-4 54-55 0.0 0.0 0.0 4.72695280748152 4.051769563605718E-4 56-57 0.0 0.0 0.0 5.351408415878614 4.1816339726956445E-4 58-59 0.0 0.0 0.0 6.068382026599555 4.20760685451363E-4 60-61 0.0 0.0 0.0 6.805487218017617 4.20760685451363E-4 62-63 0.0 0.0 0.0 7.531122784824856 4.4673356726934835E-4 64-65 0.0 0.0 0.0 8.234522967507717 4.519281436329454E-4 66-67 0.0 0.0 0.0 8.973713781335762 4.7270644908733376E-4 68-69 0.0 0.0 0.0 9.767777502580666 4.88290178178125E-4 70-71 0.0 0.0 0.0 10.608654746014306 5.090684836325133E-4 72-73 0.0 0.0 0.0 11.450409872853394 6.025708581772606E-4 74-75 0.0 0.0 5.194576363597074E-6 12.23428183527292 6.025708581772606E-4 76-77 0.0 0.0 5.194576363597074E-6 13.067084109749349 6.129600109044547E-4 78-79 0.0 0.0 5.194576363597074E-6 13.932362875650984 6.233491636316489E-4 80-81 0.0 0.0 5.194576363597074E-6 14.840463131805933 6.363356045406415E-4 82-83 0.0 0.0 5.194576363597074E-6 15.75495531417629 6.623084863586269E-4 84-85 5.194576363597074E-6 0.0 5.194576363597074E-6 16.603769870293505 6.649057745404254E-4 86-87 5.194576363597074E-6 0.0 5.194576363597074E-6 17.45276883387163 6.752949272676196E-4 88 5.194576363597074E-6 0.0 5.194576363597074E-6 18.090065435039538 6.752949272676196E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 35140 0.0 51.62007 1 GTATCAA 65915 0.0 43.14055 1 CAACGCA 84085 0.0 32.90257 5 ATCAACG 84465 0.0 32.66481 3 TATCAAC 89680 0.0 31.59261 2 GTACTAG 12630 0.0 30.898176 1 AACGCAG 92570 0.0 30.570839 6 TCAACGC 95155 0.0 29.168303 4 TAGGCAT 16165 0.0 27.96275 5 ACGCAGA 100165 0.0 27.63362 7 TATTCTC 10615 0.0 27.444304 5 CGCAGAG 101795 0.0 27.135674 8 AGGGCTA 22245 0.0 26.804626 5 GCTATTG 22095 0.0 26.687834 8 GTACATG 97525 0.0 26.655617 1 CTATTGA 22275 0.0 26.619827 9 ATTTAGG 22745 0.0 26.294159 1 TACATGG 98280 0.0 26.179104 2 ACATGGG 98470 0.0 25.441902 3 CTAGGCA 17710 0.0 25.39468 4 >>END_MODULE