FastQCFastQC Report
Mon 6 Jun 2016
ERR1211373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15347205
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412150.26855052760421194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294010.19157234167393997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211380.13773191926477818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228850.047.0215031
GTATCAA412200.042.164991
TACCTGG123000.036.964762
CAACGCA511300.033.2640085
ATCAACG511700.033.164513
TATCAAC531200.032.3142132
TCAACGC537200.031.7214034
AACGCAG550650.031.467656
GTACATG577250.029.9620691
ACCTGGG152150.029.4752983
TACATGG585150.029.0292342
ACGCAGA607400.028.0325477
ACATGGG590300.027.5858843
CGCAGAG622750.027.2715958
ATGGGGG373750.026.652115
CATGGGG469900.026.640734
GTACTAG59650.025.7488881
TGGGGGG382400.025.102826
GCAGAGT682200.024.2813059
GTACCTG205250.022.6561071
Produced by FastQC (version 0.11.5)