FastQCFastQC Report
Mon 6 Jun 2016
ERR1211373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15347205
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367470.23943773475365712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281280.1832776717324099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207240.13503435967656652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224550.046.860331
GTATCAA409550.042.075121
TACCTGG121800.037.231132
CAACGCA523900.032.295885
ATCAACG524050.032.201383
TATCAAC541350.031.614622
ACCTGGG145600.030.8874723
AACGCAG563300.030.6195356
TCAACGC553950.030.539834
GTACATG562850.028.1678181
TACATGG568700.027.5409092
ACGCAGA622100.027.2118517
CGCAGAG633000.026.6319338
TGGGGGG344900.026.5232496
ACATGGG574800.026.202523
CATGGGG453650.025.669364
ATGGGGG353850.025.6011925
GTACTAG60550.023.8386971
GCAGAGT701050.023.6111769
CCTGGGG153050.022.7827014
Produced by FastQC (version 0.11.5)