##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211373_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15347205 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.966741045030673 31.0 31.0 34.0 30.0 34.0 2 32.11587302052719 33.0 31.0 34.0 30.0 34.0 3 32.18929003685036 34.0 31.0 34.0 30.0 34.0 4 35.86285672211976 37.0 35.0 37.0 35.0 37.0 5 35.712977770219396 37.0 35.0 37.0 33.0 37.0 6 35.739845789510206 37.0 35.0 37.0 33.0 37.0 7 35.66512228122319 37.0 35.0 37.0 33.0 37.0 8 35.64614410246035 37.0 35.0 37.0 33.0 37.0 9 37.20851985752454 39.0 37.0 39.0 33.0 39.0 10-11 37.17911574127015 39.0 37.0 39.0 33.0 39.0 12-13 37.085908313598466 39.0 37.0 39.0 33.0 39.0 14-15 38.25601700765709 40.0 38.0 41.0 33.0 41.0 16-17 38.24402870099148 40.0 38.0 41.0 33.0 41.0 18-19 38.242130244562446 40.0 38.0 41.0 33.5 41.0 20-21 38.20165580638299 40.0 38.0 41.0 33.0 41.0 22-23 38.10227963332737 40.0 37.0 41.0 33.0 41.0 24-25 37.97713482683004 40.0 37.0 41.0 33.0 41.0 26-27 37.84505569580911 40.0 37.0 41.0 33.0 41.0 28-29 37.76998404595494 40.0 37.0 41.0 33.0 41.0 30-31 37.82023524804679 40.0 37.0 41.0 33.0 41.0 32-33 37.6704964193806 40.0 37.0 41.0 33.0 41.0 34-35 37.566050235205694 40.0 36.5 41.0 32.0 41.0 36-37 37.42939163841234 40.0 36.0 41.0 32.0 41.0 38-39 37.31794059569805 39.5 36.0 41.0 31.0 41.0 40-41 37.25621508932734 39.0 36.0 41.0 31.0 41.0 42-43 37.131288237825714 39.0 36.0 41.0 31.0 41.0 44-45 36.98578105915703 39.0 36.0 41.0 31.0 41.0 46-47 36.777641955000924 39.0 35.0 41.0 30.0 41.0 48-49 36.57293865560537 39.0 35.0 40.5 30.0 41.0 50-51 36.397923107171636 39.0 35.0 40.0 30.0 41.0 52-53 36.19238682222594 38.5 35.0 40.0 29.0 41.0 54-55 35.99420024688534 38.0 35.0 40.0 29.0 41.0 56-57 35.76399644104578 38.0 34.5 40.0 28.0 41.0 58-59 35.50137526670166 38.0 34.0 40.0 28.0 41.0 60-61 35.24095840252346 37.5 34.0 40.0 27.5 41.0 62-63 34.942313111735984 37.0 34.0 40.0 27.0 41.0 64-65 34.589672223704575 36.5 33.0 39.0 26.0 41.0 66-67 34.23979815868752 36.0 33.0 39.0 26.0 40.0 68-69 33.88261784474763 35.5 33.0 39.0 26.0 40.0 70-71 33.518740904288435 35.0 32.0 38.0 25.5 40.0 72-73 33.16496241498045 35.0 32.0 37.5 25.0 39.5 74-75 32.7999380343196 35.0 32.0 37.0 25.0 39.0 76-77 31.823298737457407 34.0 30.5 36.0 23.5 38.0 78-79 32.33766014723854 34.5 31.0 36.0 25.0 38.0 80-81 32.24521458467519 34.5 31.5 35.5 25.0 37.5 82-83 31.979687636934543 34.0 31.0 35.0 24.5 37.0 84-85 31.707685731701638 34.0 31.0 35.0 24.0 36.5 86-87 31.458706389860566 34.0 31.0 35.0 23.5 36.0 88-89 31.2350543307397 34.0 31.0 35.0 23.0 36.0 90-91 31.01880593241571 34.0 30.5 35.0 22.5 35.0 92-93 30.836112992561187 34.0 30.0 35.0 20.5 35.0 94-95 30.665305930298057 34.0 30.0 35.0 20.0 35.0 96-97 30.491780457744586 34.0 29.5 35.0 20.0 35.0 98-99 30.30218000606625 34.0 29.0 35.0 19.5 35.0 100 28.457033642282095 32.0 25.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3615171628539571 1101 2 -0.31563829013082767 1101 3 -0.35900892716243504 1101 4 -0.17595299704178302 1101 5 -0.2093871247383845 1101 6 -0.300679089761303 1101 7 -0.2750217730547462 1101 8 -0.3808385614166099 1101 9 -0.5190385219502502 1101 10-11 -0.47672910254233614 1101 12-13 -0.5329375634804236 1101 14-15 -0.7015232310146686 1101 16-17 -0.6951411356109318 1101 18-19 -0.636582401740732 1101 20-21 -0.7132109648246825 1101 22-23 -0.7775360235855828 1101 24-25 -0.7869062753543972 1101 26-27 -0.8160010104393294 1101 28-29 -0.8455262917324973 1101 30-31 -0.7441260920671127 1101 32-33 -0.623818815593836 1101 34-35 -0.7750063018173705 1101 36-37 -0.7656014241348501 1101 38-39 -0.7583213481945847 1101 40-41 -0.7950199316330142 1101 42-43 -0.7577973693798654 1101 44-45 -0.8109148446945653 1101 46-47 -0.9012457986583158 1101 48-49 -0.8933346228829393 1101 50-51 -0.8960133824165482 1101 52-53 -0.9336657849663439 1101 54-55 -0.9461788458527067 1101 56-57 -0.9358395420279706 1101 58-59 -0.9457466029513455 1101 60-61 -0.9297104643183047 1101 62-63 -0.9617625438892574 1101 64-65 -1.0271013319771995 1101 66-67 -1.0097151285692334 1101 68-69 -1.0585528586231518 1101 70-71 -1.0283616943142775 1101 72-73 -1.101355253098621 1101 74-75 -1.1435409173732225 1101 76-77 -0.9460584589182552 1101 78-79 -0.8576678199310095 1101 80-81 -0.7621535680162665 1101 82-83 -0.8664515422327952 1101 84-85 -0.8264275541786823 1101 86-87 -0.9056997157474633 1101 88-89 -0.9090371657184662 1101 90-91 -1.0598932804238075 1101 92-93 -1.1842260335305816 1101 94-95 -1.2156234038152043 1101 96-97 -1.1672542973630264 1101 98-99 -1.3554524624874844 1101 100 -1.1936820005581232 1102 1 -0.33174670292496344 1102 2 -0.28701028742477774 1102 3 -0.3536818929154357 1102 4 -0.1948427343347916 1102 5 -0.2503146648934731 1102 6 -0.3095484273792124 1102 7 -0.34509338533351297 1102 8 -0.34212623454427415 1102 9 -0.4744208517847355 1102 10-11 -0.5300204656796552 1102 12-13 -0.5538812787180944 1102 14-15 -0.7040156342899095 1102 16-17 -0.7251804514796802 1102 18-19 -0.7579608997964016 1102 20-21 -0.7116254589278199 1102 22-23 -0.7485644894202608 1102 24-25 -0.799412348883088 1102 26-27 -0.8158897292729819 1102 28-29 -0.8395426725287862 1102 30-31 -0.8091675669669911 1102 32-33 -0.7886707815557514 1102 34-35 -0.8249789769556344 1102 36-37 -0.8095083931600087 1102 38-39 -0.8129915507728427 1102 40-41 -0.847118091089051 1102 42-43 -0.861065379117413 1102 44-45 -0.8941505596267092 1102 46-47 -0.9808121635527058 1102 48-49 -1.005770782634876 1102 50-51 -1.059970947064734 1102 52-53 -1.062741919015302 1102 54-55 -1.1071803490711218 1102 56-57 -1.0190047334235501 1102 58-59 -1.1053606618945793 1102 60-61 -1.1457299337887576 1102 62-63 -1.1192080341698514 1102 64-65 -1.0791443623262253 1102 66-67 -1.1707095613818836 1102 68-69 -1.1745670949904508 1102 70-71 -1.2983636515630508 1102 72-73 -1.2237244203047126 1102 74-75 -1.210039367219352 1102 76-77 -1.026120133315498 1102 78-79 -0.9436622066105187 1102 80-81 -0.8558707232235676 1102 82-83 -0.9672330055985867 1102 84-85 -0.9896570174680939 1102 86-87 -1.0703600185541866 1102 88-89 -1.0333329934208848 1102 90-91 -0.9545363218127001 1102 92-93 -1.1155862135188421 1102 94-95 -1.1482579617601978 1102 96-97 -1.2035835565112727 1102 98-99 -1.1969482265823217 1102 100 -1.2159982269728538 1103 1 -0.3975236536363056 1103 2 -0.38151495514997436 1103 3 -0.41730674411356006 1103 4 -0.2510339876656502 1103 5 -0.2594697405261783 1103 6 -0.37441243355071663 1103 7 -0.3764577685148467 1103 8 -0.38329000968198557 1103 9 -0.543929447805688 1103 10-11 -0.49636668218371227 1103 12-13 -0.6060328141919769 1103 14-15 -0.7545751700198622 1103 16-17 -0.851593270117327 1103 18-19 -0.7967530534631351 1103 20-21 -0.734036373397366 1103 22-23 -0.8983324761172469 1103 24-25 -0.8202534076598198 1103 26-27 -0.868703503791842 1103 28-29 -0.9028609727025483 1103 30-31 -0.8437695615494292 1103 32-33 -0.8534981815231504 1103 34-35 -0.8484149112469908 1103 36-37 -0.8189492461612602 1103 38-39 -0.8553627850495218 1103 40-41 -0.9408561417543595 1103 42-43 -0.9311859629404395 1103 44-45 -0.901219291667104 1103 46-47 -0.9083539005168149 1103 48-49 -1.026260190293776 1103 50-51 -1.051793147706256 1103 52-53 -1.0665249093225526 1103 54-55 -1.0560467765236297 1103 56-57 -1.0424406984527437 1103 58-59 -1.1870728303982645 1103 60-61 -1.1595264947492296 1103 62-63 -1.1880544116377152 1103 64-65 -1.1277910772463784 1103 66-67 -1.1208171494517316 1103 68-69 -1.1456543694884473 1103 70-71 -1.1548634661729054 1103 72-73 -1.214135638894433 1103 74-75 -1.239112559606994 1103 76-77 -1.12964021674291 1103 78-79 -0.999289815074718 1103 80-81 -1.006674800487179 1103 82-83 -0.9853348055490265 1103 84-85 -0.9556155606691412 1103 86-87 -0.9564814808031628 1103 88-89 -0.9694569116231513 1103 90-91 -1.134202970671911 1103 92-93 -1.0634503722729711 1103 94-95 -1.1334752489746407 1103 96-97 -1.2684403777130377 1103 98-99 -1.3387258660671506 1103 100 -1.4156260767905628 1104 1 -0.4438695106664916 1104 2 -0.3774352552325233 1104 3 -0.41746816078544313 1104 4 -0.2457136379855953 1104 5 -0.26660922422217226 1104 6 -0.41156837616007635 1104 7 -0.3994754282655961 1104 8 -0.40443807913040075 1104 9 -0.5229111512388087 1104 10-11 -0.5479744686172268 1104 12-13 -0.6206790799089887 1104 14-15 -0.8090497707001347 1104 16-17 -0.8447229228191517 1104 18-19 -0.8535241931341417 1104 20-21 -0.784287899601523 1104 22-23 -0.8941173908899245 1104 24-25 -0.909921136747549 1104 26-27 -0.9330815550445735 1104 28-29 -0.9138429617582631 1104 30-31 -0.9081340894262482 1104 32-33 -0.8989649660760648 1104 34-35 -0.9314805352851181 1104 36-37 -0.8928951515462202 1104 38-39 -1.0240618100176562 1104 40-41 -1.0890217491067418 1104 42-43 -0.9379831971195998 1104 44-45 -1.016456211160289 1104 46-47 -1.0543661413695062 1104 48-49 -1.0319733846985457 1104 50-51 -1.0670699351421788 1104 52-53 -1.0460150729988413 1104 54-55 -1.0380258374678846 1104 56-57 -1.0507378275819903 1104 58-59 -1.0910754541453613 1104 60-61 -1.1479171741172607 1104 62-63 -1.1588969422053665 1104 64-65 -1.091684833018256 1104 66-67 -1.2198692123272963 1104 68-69 -1.14490625451419 1104 70-71 -1.1344216559157232 1104 72-73 -1.0096505155425035 1104 74-75 -1.0054340568796079 1104 76-77 -0.9552824545954017 1104 78-79 -0.9088155835720038 1104 80-81 -1.0345742347634967 1104 82-83 -1.0244862190146016 1104 84-85 -0.9416528821556547 1104 86-87 -0.8490762908073073 1104 88-89 -0.9868718877541625 1104 90-91 -1.00767521185902 1104 92-93 -1.2297190339129038 1104 94-95 -1.2489738738286675 1104 96-97 -1.2756782456106173 1104 98-99 -1.1822875393548138 1104 100 -1.2672509303609942 1105 1 -0.42763225390629955 1105 2 -0.40882256450092314 1105 3 -0.4595208785547342 1105 4 -0.29402764607569054 1105 5 -0.31055506819001266 1105 6 -0.38204165293326753 1105 7 -0.4026590592795287 1105 8 -0.4336476715785125 1105 9 -0.613979154686497 1105 10-11 -0.6211943493257692 1105 12-13 -0.6282170384394945 1105 14-15 -0.8185522984700029 1105 16-17 -0.7783433984011907 1105 18-19 -0.7460074193921571 1105 20-21 -0.8518739916206854 1105 22-23 -0.8792669572063545 1105 24-25 -0.8686403653205872 1105 26-27 -0.8753020240602538 1105 28-29 -0.9237466203689806 1105 30-31 -0.8820453579689769 1105 32-33 -0.8173352095103326 1105 34-35 -0.8029921180877153 1105 36-37 -0.9532585705976331 1105 38-39 -0.898246100130649 1105 40-41 -0.8928064629859094 1105 42-43 -0.9366749013052313 1105 44-45 -0.9630115172848832 1105 46-47 -1.0469511159520337 1105 48-49 -0.9783792936934006 1105 50-51 -0.961183283992419 1105 52-53 -0.8959167184638801 1105 54-55 -1.0023646737178424 1105 56-57 -1.04568225264412 1105 58-59 -1.128668367932903 1105 60-61 -1.0728935194041185 1105 62-63 -1.0722842553954806 1105 64-65 -1.0807094156688706 1105 66-67 -1.1195393485530047 1105 68-69 -1.155519408711747 1105 70-71 -1.1367269666533488 1105 72-73 -1.1859971951166308 1105 74-75 -1.0927699954366332 1105 76-77 -0.9992827927676764 1105 78-79 -0.9680866071303811 1105 80-81 -0.8524831943226481 1105 82-83 -0.9217691636735594 1105 84-85 -0.9155173365139859 1105 86-87 -0.9357731863345506 1105 88-89 -0.9056140729252569 1105 90-91 -0.9550060139784158 1105 92-93 -1.0045901717238372 1105 94-95 -0.9681587625055812 1105 96-97 -1.0926293017855748 1105 98-99 -1.109940634332503 1105 100 -1.154716478997745 1106 1 -0.4018339971724032 1106 2 -0.37106802124340277 1106 3 -0.4067069127928882 1106 4 -0.24139933355122878 1106 5 -0.3046625724922478 1106 6 -0.3560553670542177 1106 7 -0.3652455223396984 1106 8 -0.3991369366185751 1106 9 -0.5458937728721338 1106 10-11 -0.5384002089498594 1106 12-13 -0.6469588686594179 1106 14-15 -0.7579572934347638 1106 16-17 -0.8005150714746208 1106 18-19 -0.7762079398968424 1106 20-21 -0.7258336073972842 1106 22-23 -0.7445255783111264 1106 24-25 -0.8749339298933023 1106 26-27 -0.799060082439091 1106 28-29 -0.7994850235374997 1106 30-31 -0.8313720337740875 1106 32-33 -0.8191039848464712 1106 34-35 -0.8200706026565001 1106 36-37 -0.8325237961882408 1106 38-39 -0.8483240223279296 1106 40-41 -0.8895828132390946 1106 42-43 -0.7635569679656271 1106 44-45 -0.8816398636880223 1106 46-47 -0.8723396288144087 1106 48-49 -0.8888245542083908 1106 50-51 -0.9356045347372444 1106 52-53 -0.9346164965829402 1106 54-55 -0.9763697055091711 1106 56-57 -1.022639076069126 1106 58-59 -0.9850663490077167 1106 60-61 -1.067456606465086 1106 62-63 -0.9955328526627127 1106 64-65 -1.0611495496495351 1106 66-67 -0.9815344916793265 1106 68-69 -1.0515325205938524 1106 70-71 -0.946194387128088 1106 72-73 -1.04557986789505 1106 74-75 -1.0640824490871843 1106 76-77 -0.9683030967328072 1106 78-79 -0.8971839046844039 1106 80-81 -0.8273625235070909 1106 82-83 -0.8230341243455612 1106 84-85 -0.7873380975431523 1106 86-87 -0.8164338177555059 1106 88-89 -0.8469135047924219 1106 90-91 -0.8700071705224097 1106 92-93 -0.8761691010298058 1106 94-95 -0.852833101447743 1106 96-97 -0.9487890027770938 1106 98-99 -0.9770126865954332 1106 100 -1.1175848342259727 1107 1 -0.3720644964001174 1107 2 -0.33643534379936924 1107 3 -0.4457097427110668 1107 4 -0.23086710768071583 1107 5 -0.2762854176920939 1107 6 -0.34816653960792365 1107 7 -0.3807770580757506 1107 8 -0.3916412408159289 1107 9 -0.5142145489776269 1107 10-11 -0.49321933939653206 1107 12-13 -0.5209247883972665 1107 14-15 -0.7029423854179342 1107 16-17 -0.6718488034786603 1107 18-19 -0.7290789714373034 1107 20-21 -0.7151177709643903 1107 22-23 -0.7682193393654444 1107 24-25 -0.7518778549714256 1107 26-27 -0.7806842174837882 1107 28-29 -0.7928569206043292 1107 30-31 -0.8387232357075689 1107 32-33 -0.8477245579167914 1107 34-35 -0.8450824530616785 1107 36-37 -0.7838529340816436 1107 38-39 -0.7909243973605555 1107 40-41 -0.7646352254655611 1107 42-43 -0.7888230922630655 1107 44-45 -0.8156595866916518 1107 46-47 -0.9542183360087577 1107 48-49 -1.035833438562186 1107 50-51 -0.9601344887859611 1107 52-53 -1.000241269709072 1107 54-55 -0.9080281366028728 1107 56-57 -0.9458862534949404 1107 58-59 -0.8887900569975429 1107 60-61 -0.9835685487122277 1107 62-63 -0.9714554923655854 1107 64-65 -0.9199765776678106 1107 66-67 -0.8823213751525998 1107 68-69 -0.8548963076635445 1107 70-71 -0.883071768615693 1107 72-73 -0.919452246067614 1107 74-75 -0.8557240390537686 1107 76-77 -0.8897827109231464 1107 78-79 -0.7768634962403169 1107 80-81 -0.7958521345986256 1107 82-83 -0.7210524667488798 1107 84-85 -0.7835168948940883 1107 86-87 -0.806734591631642 1107 88-89 -0.7643031414512471 1107 90-91 -0.7359752970808735 1107 92-93 -0.7284639774105166 1107 94-95 -0.7491559665858603 1107 96-97 -0.8302772508086953 1107 98-99 -0.9300971994124652 1107 100 -1.021794957408403 1108 1 -0.4543581957153435 1108 2 -0.4491693450790919 1108 3 -0.4611406230468127 1108 4 -0.2888114479639654 1108 5 -0.35581573302098946 1108 6 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2311 58-59 1.3214656657449666 2311 60-61 1.281595062880001 2311 62-63 1.3330711070717314 2311 64-65 1.3062522257706135 2311 66-67 1.340373809163026 2311 68-69 1.3747902042933546 2311 70-71 1.3945257438497052 2311 72-73 1.382628352305261 2311 74-75 1.2878805777109008 2311 76-77 1.1839414933779686 2311 78-79 1.1758033872608138 2311 80-81 1.1768064657580126 2311 82-83 1.1627889360929942 2311 84-85 1.184981847997566 2311 86-87 1.2540286350852092 2311 88-89 1.2710287966814136 2311 90-91 1.3196074220143394 2311 92-93 1.3016350137104702 2311 94-95 1.3716949342328526 2311 96-97 1.4370726738763615 2311 98-99 1.4661312314069121 2311 100 1.5139358754788361 2312 1 0.1878724427982199 2312 2 0.1846038353696855 2312 3 0.3757389944696854 2312 4 0.29462643755371687 2312 5 0.3804141835471171 2312 6 0.4690133024386469 2312 7 0.5084680313146492 2312 8 0.32629378833591005 2312 9 0.5072170656430615 2312 10-11 0.366802936677324 2312 12-13 0.5347752667477224 2312 14-15 0.8806552905189804 2312 16-17 0.9636888986650192 2312 18-19 0.9464924730725244 2312 20-21 0.9509665992609868 2312 22-23 0.6173074047584493 2312 24-25 0.5590658319580015 2312 26-27 0.8655586774292274 2312 28-29 1.0048434651556448 2312 30-31 0.9458373701532281 2312 32-33 0.8281233327799953 2312 34-35 0.9781125892435867 2312 36-37 1.0766740921515492 2312 38-39 1.0564695225473884 2312 40-41 1.095320238751917 2312 42-43 1.0181936445541453 2312 44-45 0.9559696994930249 2312 46-47 1.1008076976849281 2312 48-49 1.1549197776789484 2312 50-51 1.1788667240081239 2312 52-53 1.2346522527244517 2312 54-55 1.2182589340584045 2312 56-57 1.2379317188868129 2312 58-59 1.254428858320054 2312 60-61 1.28805525513242 2312 62-63 1.2958120090820202 2312 64-65 1.362632206677425 2312 66-67 1.3245276032016378 2312 68-69 1.3102739827423378 2312 70-71 1.1570410290555273 2312 72-73 1.2647543406832327 2312 74-75 1.2948220931629564 2312 76-77 1.192972235537642 2312 78-79 1.136548122853398 2312 80-81 0.9983990010649748 2312 82-83 1.0278877166208389 2312 84-85 0.9092522849088347 2312 86-87 1.0653570398303494 2312 88-89 0.754244406468942 2312 90-91 1.0898458660189654 2312 92-93 1.2107722148327866 2312 94-95 1.2944174492828537 2312 96-97 1.3600351384824307 2312 98-99 1.3634036720246065 2312 100 1.450023076552153 2313 1 -0.30579222662164085 2313 2 0.04298565371883001 2313 3 0.305922959055394 2313 4 0.16073156903544827 2313 5 0.29181432933381757 2313 6 0.40988938875756986 2313 7 0.23074458492072836 2313 8 0.16149963168415837 2313 9 0.32596392085480375 2313 10-11 0.4056367676192423 2313 12-13 0.6180127161592068 2313 14-15 0.6690698681498688 2313 16-17 0.8404310799893651 2313 18-19 0.7320025481673724 2313 20-21 0.949130715198109 2313 22-23 0.973541851128715 2313 24-25 0.8485642888753944 2313 26-27 1.0513103693701282 2313 28-29 0.9481167107731423 2313 30-31 0.9220189141632886 2313 32-33 1.0547736877810223 2313 34-35 1.067705827539477 2313 36-37 0.9277339649760137 2313 38-39 0.7060141497371433 2313 40-41 0.8525010437890259 2313 42-43 0.8755373555991142 2313 44-45 1.0878908615486083 2313 46-47 1.119049225012823 2313 48-49 1.1265316545148707 2313 50-51 1.076039135075014 2313 52-53 1.0437357652136185 2313 54-55 1.13142551562904 2313 56-57 1.1819081929424442 2313 58-59 1.1294447077541037 2313 60-61 1.1439286409671396 2313 62-63 1.1898511174597033 2313 64-65 1.2800834390041658 2313 66-67 1.2780103792635416 2313 68-69 1.2344784857683848 2313 70-71 1.1304867790826023 2313 72-73 1.207365824248832 2313 74-75 0.903239878627268 2313 76-77 1.0455492289180803 2313 78-79 0.8913653767424137 2313 80-81 1.0255837205791352 2313 82-83 1.0489424116746804 2313 84-85 1.0058584548130973 2313 86-87 1.099795937296566 2313 88-89 1.1279982680597698 2313 90-91 1.2273925194383786 2313 92-93 0.9596115130750036 2313 94-95 0.9725201825385952 2313 96-97 1.2494267144297986 2313 98-99 1.3326699506438224 2313 100 1.4128724483355057 2314 1 -0.3180067808762068 2314 2 -0.11604690100994475 2314 3 0.1641277140235431 2314 4 -0.09510954466438903 2314 5 0.23282770432334843 2314 6 0.3120872358108997 2314 7 0.20676272325507483 2314 8 0.25048670392372685 2314 9 0.3093911900455453 2314 10-11 0.473659168472814 2314 12-13 0.5706359011544677 2314 14-15 0.6672251457371559 2314 16-17 0.8228728213006988 2314 18-19 0.8992815726258385 2314 20-21 0.5365813780200241 2314 22-23 0.739266479396143 2314 24-25 0.9685037496732747 2314 26-27 0.9353113163599929 2314 28-29 0.863280173007837 2314 30-31 0.8676809694010572 2314 32-33 0.9754272243693336 2314 34-35 1.0133774396370967 2314 36-37 0.9207488321489734 2314 38-39 0.892939203014194 2314 40-41 0.9396172076424065 2314 42-43 0.7908002819397666 2314 44-45 0.8514755607054028 2314 46-47 0.9537643276554562 2314 48-49 0.8681360196838241 2314 50-51 0.9949872646635427 2314 52-53 1.0878299541459029 2314 54-55 1.1259083945634103 2314 56-57 0.9894463103493081 2314 58-59 0.9794196398533757 2314 60-61 1.103555123034674 2314 62-63 0.793332148202083 2314 64-65 0.94689415958252 2314 66-67 1.0504288735354592 2314 68-69 1.1091461785773689 2314 70-71 1.2373366517287891 2314 72-73 1.1817069777023619 2314 74-75 1.165427998447747 2314 76-77 0.9591235193500829 2314 78-79 0.9370366605170801 2314 80-81 0.991711591541474 2314 82-83 0.9453841274300601 2314 84-85 0.8500919591583163 2314 86-87 1.005988658252594 2314 88-89 1.0629129910545885 2314 90-91 1.1675391928347771 2314 92-93 1.2053980914458684 2314 94-95 1.249899849516197 2314 96-97 1.3402573643885525 2314 98-99 1.3496674248642577 2314 100 1.347768155220134 2315 1 -0.024434514481345815 2315 2 0.14445282092788148 2315 3 0.20640744281941892 2315 4 0.0011471198269532579 2315 5 0.2913412770546415 2315 6 0.41259301417932903 2315 7 0.47282097381349786 2315 8 0.4095008584708282 2315 9 0.6523924298777501 2315 10-11 0.6290821847500183 2315 12-13 0.70441963053851 2315 14-15 0.8487039797472633 2315 16-17 0.8201238595334601 2315 18-19 0.8901626597726136 2315 20-21 0.8648762312308165 2315 22-23 0.9552124418903176 2315 24-25 0.9075066704620838 2315 26-27 0.9784565187593088 2315 28-29 1.0279067616702875 2315 30-31 0.9981023690377384 2315 32-33 1.0539832509547153 2315 34-35 1.07366851590956 2315 36-37 1.0891628600213679 2315 38-39 1.1173123717682785 2315 40-41 1.0694303339012805 2315 42-43 1.116855843340474 2315 44-45 1.0093752498793123 2315 46-47 1.0291775147582598 2315 48-49 1.1456429164762056 2315 50-51 1.084971829970172 2315 52-53 1.097556618973968 2315 54-55 1.0517383574197154 2315 56-57 1.1436767993623889 2315 58-59 1.2161088421976984 2315 60-61 1.2569813290164689 2315 62-63 1.231192701276072 2315 64-65 1.3041652814425007 2315 66-67 1.289432912864747 2315 68-69 1.1499527313136966 2315 70-71 0.791727412444672 2315 72-73 1.0752859961201793 2315 74-75 1.2665320066326657 2315 76-77 1.1539813149887728 2315 78-79 1.0536408753407045 2315 80-81 1.0606704942672494 2315 82-83 1.0274165865424578 2315 84-85 0.9672717213123327 2315 86-87 0.9699042804529618 2315 88-89 1.0117763791626189 2315 90-91 1.108619217065165 2315 92-93 1.164876405334656 2315 94-95 1.2292735350126733 2315 96-97 1.2611016428151807 2315 98-99 1.2384688976791232 2315 100 1.1200710599415764 2316 1 0.5354105449669326 2316 2 0.5242385147480348 2316 3 0.5160777931091047 2316 4 0.3480781903383914 2316 5 0.4395475561083515 2316 6 0.4668197865020005 2316 7 0.5250696836029576 2316 8 0.5313909058649315 2316 9 0.6052245512050405 2316 10-11 0.553607415281526 2316 12-13 0.681210813937156 2316 14-15 0.8588824649818108 2316 16-17 0.880775592847094 2316 18-19 0.8995246157168424 2316 20-21 0.9103994159122308 2316 22-23 0.9466909054927655 2316 24-25 1.010825624149902 2316 26-27 1.05883249030542 2316 28-29 0.9880650507596371 2316 30-31 0.8891419955298687 2316 32-33 0.8538448646119576 2316 34-35 0.7807412737971049 2316 36-37 0.7690771881408907 2316 38-39 0.7053272901675314 2316 40-41 0.8133810979924689 2316 42-43 0.9598074439167377 2316 44-45 0.9181504219959606 2316 46-47 0.9766135424416476 2316 48-49 0.6935989899145127 2316 50-51 0.815032381495989 2316 52-53 1.0024492990495375 2316 54-55 1.0296042335418392 2316 56-57 1.0617400209920973 2316 58-59 0.9541968260204499 2316 60-61 1.056706896228988 2316 62-63 1.0992638633508847 2316 64-65 1.0705728267114054 2316 66-67 1.0368212907575511 2316 68-69 1.0230816373784464 2316 70-71 0.9253623894682761 2316 72-73 0.955317780798346 2316 74-75 1.000186353603297 2316 76-77 0.8553500374305365 2316 78-79 0.808992010817235 2316 80-81 0.8035969719826355 2316 82-83 0.7103752356343378 2316 84-85 0.816631768884033 2316 86-87 0.796202434292784 2316 88-89 0.7279260176010958 2316 90-91 0.8010129723945134 2316 92-93 0.850261821456531 2316 94-95 0.7874415161214259 2316 96-97 0.9695753349053113 2316 98-99 1.0324104041357778 2316 100 0.7181883528098787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 4.0 11 22.0 12 83.0 13 410.0 14 1582.0 15 4595.0 16 10840.0 17 22047.0 18 35825.0 19 50372.0 20 68411.0 21 92147.0 22 111094.0 23 120259.0 24 128865.0 25 145904.0 26 175408.0 27 217545.0 28 267815.0 29 327514.0 30 400638.0 31 496327.0 32 625670.0 33 813716.0 34 1127506.0 35 1513057.0 36 2231206.0 37 3260648.0 38 2726257.0 39 371420.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52312472468703 20.438471980677683 13.815627652286167 24.222775642349117 2 15.334179741522968 21.223056576099687 40.22703156698565 23.215732115391695 3 17.19700865382385 27.87370259755458 31.181673222040967 23.747615526580606 4 12.218101804893982 17.723251327296598 38.92658479878817 31.13206206902125 5 12.567338209592888 38.509952596011665 34.74466883509499 14.178040359300455 6 29.246591105454776 38.17516206860872 19.15148192465546 13.426764901281041 7 26.205257252472443 33.22212940748724 23.7031233099501 16.86949003009022 8 26.04040747604117 35.47262164465608 20.791814250975424 17.69515662832733 9 27.455050572382174 15.288904336076946 20.542885690243654 36.713159401297226 10-11 24.737069664431573 26.093539561943334 29.212572933669428 19.95681783995566 12-13 25.2274719941743 24.047706794558266 28.837298539326223 21.887522671941216 14-15 22.90763183310829 25.41881017690689 26.434493984976736 25.239064005008082 16-17 22.223070006503796 27.728746923175642 27.23849774377345 22.80968532654712 18-19 21.957157671380553 27.425182630974177 28.917063400143544 21.70059629750173 20-21 22.9125065032206 26.798722646767537 28.220330172855267 22.06844067715659 22-23 22.76476800709862 26.555846050008654 28.13939177001258 22.53999417288015 24-25 22.353953509480416 26.537382877201622 28.461773809002928 22.646889804315038 26-27 22.354183288180156 26.713205438371883 28.24672586116422 22.685885412283742 28-29 22.18310940933017 27.418451072011546 28.19377549165037 22.204664027007908 30-31 23.24395516695886 26.60682420636012 28.206585177083266 21.942635449597752 32-33 23.152790225247013 26.747889424222837 27.82374503035752 22.27557532017262 34-35 22.953439912589346 26.929210372943757 28.33298818551692 21.784361528949972 36-37 22.664741206612582 26.89520772081151 28.23994464258892 22.20010642998699 38-39 22.729483531872653 26.83916725060584 28.239490528715212 22.191858688806303 40-41 22.681464647894515 26.31400087188636 28.588555027051054 22.41597945316807 42-43 22.464184854181575 26.625404991448125 28.063464684042 22.846945470328297 44-45 21.882468704588256 27.036137158943546 28.430501704060422 22.650892432407776 46-47 21.984209061589926 26.532087690854905 28.07714127534084 23.406561972214327 48-49 22.31040856281508 26.57092514802375 28.17225653909349 22.946409750067687 50-51 22.22195613137181 26.73712252786395 27.930334721871418 23.11058661889282 52-53 21.9188928769623 27.005570395744666 27.79361034196612 23.28192638532692 54-55 21.749092725887323 26.717845196042955 28.13956984542829 23.39349223264143 56-57 22.268058575151418 26.56056122383986 27.70467017227397 23.466710028734756 58-59 21.793746127626143 26.67273191951666 28.147850589773782 23.385671363083414 60-61 22.24318985991401 27.011338618776616 27.596954075052533 23.148517446256843 62-63 22.199985958215755 26.79139202788288 27.449029707821747 23.559592306079622 64-65 21.687064283775918 26.48764594693161 27.598910499263702 24.226379270028772 66-67 21.754370079661513 26.420790466615312 27.50504048785026 24.31979896587292 68-69 21.433468203617455 26.604357410327562 27.716216623679312 24.24595776237567 70-71 21.632097816154353 26.309102189908305 27.776028334141962 24.28277165979538 72-73 21.74304817881684 26.73419413681597 27.3110431538563 24.211714530510886 74-75 21.880165685166116 26.77386394195492 27.14058563105095 24.20538474182801 76-77 21.64242046708281 26.51927706072872 27.237347593241317 24.600954878947153 78-79 21.439025048265485 26.514910004565557 27.583954836043322 24.462110111125636 80-81 21.548507377589793 26.762426400359367 27.268682329088083 24.420383892962754 82-83 21.89271496503934 26.420739544390393 27.445183328379922 24.241362162190345 84-85 21.97215612624252 26.68274482768107 26.871227698331317 24.473871347745092 86-87 21.471913832575904 26.861846602934026 27.126853127460304 24.539386437029766 88-89 21.53466610136241 26.31948787763349 27.248279165549892 24.897566855454208 90-91 21.93040135555499 26.006393728863454 27.356770096652905 24.70643481892865 92-93 21.868695506067763 26.26280842446261 27.1002335754511 24.76826249401853 94-95 21.41375142813558 26.04301222131522 27.302453001445258 25.24078334910394 96-97 21.27829485186564 25.94151098405589 27.430243868873173 25.349950295205296 98-99 21.237880722873623 26.06712807596377 27.308265491276813 25.386725709885795 100 21.171644657097996 26.093703392742757 27.36280566784654 25.37184628231271 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 896.0 1 695.5 2 1145.5 3 1798.5 4 1423.5 5 1205.0 6 1771.0 7 3254.0 8 5261.5 9 6460.5 10 6453.5 11 6462.5 12 7266.0 13 8478.0 14 9949.0 15 11500.0 16 13058.5 17 14593.5 18 16326.0 19 18048.5 20 19802.0 21 22108.0 22 25519.5 23 30509.5 24 37262.0 25 46213.5 26 58703.5 27 74342.5 28 94082.0 29 120980.0 30 152362.0 31 182906.0 32 222294.0 33 260199.0 34 292257.0 35 328221.5 36 367305.0 37 418867.5 38 448706.5 39 445805.5 40 442377.5 41 447416.0 42 463270.0 43 485894.5 44 511493.5 45 551155.0 46 589960.5 47 620615.0 48 656323.0 49 677257.0 50 684763.5 51 687063.0 52 662950.5 53 620812.0 54 578268.0 55 521247.5 56 460559.5 57 396226.0 58 329651.0 59 275336.0 60 220078.5 61 170428.0 62 133909.5 63 103163.5 64 74355.0 65 51912.0 66 37532.0 67 27901.5 68 21073.5 69 15786.0 70 12038.5 71 8988.5 72 6688.0 73 4986.0 74 3804.5 75 2798.0 76 1862.0 77 1174.0 78 730.0 79 466.5 80 306.5 81 191.0 82 112.5 83 68.5 84 56.0 85 39.5 86 20.5 87 12.0 88 13.0 89 11.0 90 8.0 91 4.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05111028359887028 2 0.0 3 6.5158444159702045E-6 4 0.016165809996022077 5 0.003948601716077944 6 6.5158444159702045E-6 7 1.628961103992551E-4 8 2.932129987186592E-4 9 0.0018961107250473294 10-11 8.242543186202307E-4 12-13 2.6714962105477835E-4 14-15 4.8868833119776536E-5 16-17 6.190052195171694E-5 18-19 0.0 20-21 9.773766623955308E-6 22-23 3.2579222079851022E-6 24-25 7.493221078365735E-5 26-27 9.773766623955308E-6 28-29 9.480553625236647E-4 30-31 8.535756184920967E-4 32-33 0.014090513549535567 34-35 0.023049799621494598 36-37 0.03078410694325123 38-39 0.020466267310562412 40-41 1.400906549433594E-4 42-43 0.0017983730588077764 44-45 0.009806345846035158 46-47 0.01393413328355228 48-49 0.005538467753574673 50-51 0.01022335988865725 52-53 0.01579766478651976 54-55 0.009851956756946948 56-57 0.002987514664722339 58-59 0.00860743047349664 60-61 0.001785341369975836 62-63 9.936662734354562E-4 64-65 0.01908490829437673 66-67 0.045115706736177694 68-69 0.054684224261029944 70-71 0.04826611751129929 72-73 0.0403265610904396 74-75 0.028480755942205765 76-77 0.028816321929628227 78-79 0.012191144902280251 80-81 0.002104617746358376 82-83 0.02765975954579352 84-85 0.04788819853517302 86-87 0.04817815361168369 88-89 0.05963300809495931 90-91 0.03451442787139417 92-93 0.025952608308809327 94-95 0.007480189389533795 96-97 0.0038899591163342124 98-99 0.022261382447162203 100 0.01885685373981777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.5347205E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.2653469815432 #Duplication Level Percentage of deduplicated Percentage of total 1 71.67159837620812 38.176125562305344 2 15.267431021442857 16.264500217478606 3 5.633783976909928 9.002563750474971 4 2.6425757014489464 5.630308466506921 5 1.3902050708660716 3.7024877737591084 6 0.8392495945292379 2.6821752514031583 7 0.535928092553079 1.998247706489745 8 0.3933541305023818 1.6761715398266077 9 0.2659842002509785 1.2750966646178978 >10 1.2665809825207053 11.42935426645724 >50 0.06001803909447256 2.1742369997899704 >100 0.02972406568580567 2.922171408767721 >500 0.002184236470398603 0.8053298678567338 >1k 0.0012761649882787331 1.1953917006742363 >5k 3.5448842824378014E-5 0.15410502783548713 >10k+ 7.089768564875603E-5 0.911733795756213 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36747 0.23943773475365712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28128 0.1832776717324099 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20724 0.13503435967656652 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.5158444159702045E-6 6.5158444159702045E-6 5 0.0 0.0 0.0 6.5158444159702045E-6 6.5158444159702045E-6 6 0.0 0.0 0.0 6.5158444159702045E-6 1.3031688831940409E-5 7 0.0 0.0 0.0 6.5158444159702045E-6 1.3031688831940409E-5 8 0.0 0.0 0.0 6.5158444159702045E-6 1.3031688831940409E-5 9 0.0 0.0 0.0 6.5158444159702045E-6 2.6063377663880818E-5 10-11 6.5158444159702045E-6 0.0 0.0 3.5837144287836124E-5 2.6063377663880818E-5 12-13 6.5158444159702045E-6 0.0 0.0 4.886883311977653E-5 3.2579222079851024E-5 14-15 6.5158444159702045E-6 3.2579222079851022E-6 0.0 6.190052195171694E-5 6.515844415970205E-5 16-17 6.5158444159702045E-6 6.5158444159702045E-6 0.0 1.0425351065552327E-4 7.819013299164245E-5 18-19 6.5158444159702045E-6 6.5158444159702045E-6 0.0 1.791857214391806E-4 9.773766623955306E-5 20-21 6.5158444159702045E-6 6.5158444159702045E-6 0.0 3.6814520950231654E-4 9.773766623955306E-5 22-23 6.5158444159702045E-6 6.5158444159702045E-6 0.0 0.0012314945946183685 1.0099558844753817E-4 24-25 6.5158444159702045E-6 9.773766623955306E-6 0.0 0.003215569219281296 1.1076935507149347E-4 26-27 6.5158444159702045E-6 1.3031688831940409E-5 0.0 0.005672042564102062 1.1076935507149347E-4 28-29 6.5158444159702045E-6 1.3031688831940409E-5 0.0 0.014507527592157659 1.1076935507149347E-4 30-31 6.5158444159702045E-6 1.3031688831940409E-5 0.0 0.049360779373182284 1.1076935507149347E-4 32-33 1.9547533247910612E-5 1.3031688831940409E-5 0.0 0.12784738328575138 1.2380104390343388E-4 34-35 1.9547533247910612E-5 1.3031688831940409E-5 0.0 0.23411428986580946 1.3683273273537428E-4 36-37 1.9547533247910612E-5 1.3031688831940409E-5 0.0 0.39682795662141734 1.3683273273537428E-4 38-39 1.9547533247910612E-5 1.3031688831940409E-5 0.0 0.6849846600732837 1.433485771513445E-4 40-41 1.9547533247910612E-5 1.3031688831940409E-5 0.0 1.0480019000202316 1.433485771513445E-4 42-43 1.9547533247910612E-5 1.9547533247910612E-5 0.0 1.3920221955724186 1.433485771513445E-4 44-45 1.9547533247910612E-5 1.9547533247910612E-5 0.0 1.7664030681808187 1.466064993593296E-4 46-47 1.9547533247910612E-5 2.6063377663880818E-5 0.0 2.176376740911456 1.6941195481522532E-4 48-49 1.9547533247910612E-5 2.6063377663880818E-5 0.0 2.6373206065860204 2.0199117689507633E-4 50-51 1.9547533247910612E-5 2.6063377663880818E-5 0.0 3.1536361181074994 2.0199117689507633E-4 52-53 1.9547533247910612E-5 2.6063377663880818E-5 0.0 3.67521968983929 2.2479663235097205E-4 54-55 1.9547533247910612E-5 2.6063377663880818E-5 0.0 4.208652324641522 2.5086001001485287E-4 56-57 1.9547533247910612E-5 2.6063377663880818E-5 0.0 4.7613588272261955 2.606337766388082E-4 58-59 1.9547533247910612E-5 2.6063377663880818E-5 0.0 5.403081538299645 2.704075432627635E-4 60-61 1.9547533247910612E-5 2.6063377663880818E-5 0.0 6.054685527429913 3.0624468755059963E-4 62-63 1.9547533247910612E-5 2.6063377663880818E-5 0.0 6.733978597405847 3.1927637638254E-4 64-65 1.9547533247910612E-5 2.6063377663880818E-5 0.0 7.368221119089762 3.225342985905251E-4 66-67 1.9547533247910612E-5 2.6063377663880818E-5 0.0 8.037759969974989 3.323080652144804E-4 68-69 1.9547533247910612E-5 2.6063377663880818E-5 0.0 8.772535455152909 3.3882390963045064E-4 70-71 1.9547533247910612E-5 2.6063377663880818E-5 0.0 9.542216318867181 3.51855598462391E-4 72-73 1.9547533247910612E-5 2.6063377663880818E-5 0.0 10.278422031894406 3.9746650937418245E-4 74-75 1.9547533247910612E-5 2.6063377663880818E-5 0.0 10.97841268165767 3.9746650937418245E-4 76-77 1.9547533247910612E-5 2.6063377663880818E-5 0.0 11.714080837520578 4.0398235379015266E-4 78-79 1.9547533247910612E-5 2.6063377663880818E-5 0.0 12.503898918402406 4.0398235379015266E-4 80-81 1.9547533247910612E-5 2.6063377663880818E-5 0.0 13.277472999155222 4.300457314540335E-4 82-83 1.9547533247910612E-5 2.6063377663880818E-5 0.0 14.075921315966003 4.561091091179143E-4 84-85 1.9547533247910612E-5 2.6063377663880818E-5 0.0 14.846726162842028 4.561091091179143E-4 86-87 1.9547533247910612E-5 2.6063377663880818E-5 0.0 15.60234257638443 4.593670313258994E-4 88 1.9547533247910612E-5 2.6063377663880818E-5 0.0 16.16771262259154 4.821724867817951E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22455 0.0 46.86033 1 GTATCAA 40955 0.0 42.07512 1 TACCTGG 12180 0.0 37.23113 2 CAACGCA 52390 0.0 32.29588 5 ATCAACG 52405 0.0 32.20138 3 TATCAAC 54135 0.0 31.61462 2 ACCTGGG 14560 0.0 30.887472 3 AACGCAG 56330 0.0 30.619535 6 TCAACGC 55395 0.0 30.53983 4 GTACATG 56285 0.0 28.167818 1 TACATGG 56870 0.0 27.540909 2 ACGCAGA 62210 0.0 27.211851 7 CGCAGAG 63300 0.0 26.631933 8 TGGGGGG 34490 0.0 26.523249 6 ACATGGG 57480 0.0 26.20252 3 CATGGGG 45365 0.0 25.66936 4 ATGGGGG 35385 0.0 25.601192 5 GTACTAG 6055 0.0 23.838697 1 GCAGAGT 70105 0.0 23.611176 9 CCTGGGG 15305 0.0 22.782701 4 >>END_MODULE