FastQCFastQC Report
Mon 6 Jun 2016
ERR1211372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10985929
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369090.33596612539549453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270420.2461512358217498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193550.1761799115941856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84350.037.663892
GTATCAA440500.031.8531861
ACCTGGG99600.030.819953
GGTATCA288700.030.763711
GTACATG429950.029.3252871
TACATGG435400.028.462232
ACATGGG435450.027.3664053
CAACGCA520250.026.5742515
GTACTAG47050.026.437381
ATCAACG524500.026.3499583
TATCAAC540050.025.8054222
CATGGGG318500.025.6961154
AACGCAG551550.025.45036
TCAACGC547900.025.3361284
ATGGGGG242900.024.2881435
TGGGGGG256150.023.6012276
GTACCTG138500.023.4392431
ACGCAGA592650.023.3604357
CGCAGAG600250.022.9772558
CCTGGGG105450.021.5317154
Produced by FastQC (version 0.11.5)