FastQCFastQC Report
Mon 6 Jun 2016
ERR1211372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10985929
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323320.2943037407214265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254690.23183292009260212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190050.17299401807530343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG85150.037.031462
GGTATCA294000.030.8981231
GTATCAA442150.030.647611
ACCTGGG101000.030.196893
GTACATG411850.027.1256181
TACATGG419600.026.1843622
ACATGGG413500.025.491343
CAACGCA528550.025.0792435
ATCAACG531500.024.9512713
TGGGGGG229950.024.6633196
TATCAAC548100.024.589652
CATGGGG309450.024.2377994
TCAACGC553700.024.019434
AACGCAG559900.023.9676466
ATGGGGG229550.023.6020345
CCTGGGG98950.022.749434
GTACTAG48750.022.665511
GTACCTG142900.022.6045971
ACGCAGA606550.021.7601937
GGGCTAT88450.021.7271696
Produced by FastQC (version 0.11.5)