FastQCFastQC Report
Mon 6 Jun 2016
ERR1211371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10331823
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319860.3095871851463193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238630.2309660163554873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168010.1626140904659323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207650.037.13891
GTATCAA333650.035.567171
TACCTGG78850.033.5427132
CAACGCA401600.029.0761875
ATCAACG403500.028.9280433
TATCAAC418150.028.1928652
TCAACGC419350.027.9799884
GTACATG349450.027.9329621
AACGCAG426500.027.787286
ACCTGGG96250.027.0579513
TACATGG353950.026.8978482
ACATGGG353550.025.9546343
ACGCAGA467500.025.1091067
CGCAGAG476550.024.5722478
CATGGGG266950.024.2660394
ATGGGGG199350.023.133025
GTACTAG42350.022.9153291
GCAGAGT514750.022.2724349
GTACCTG125800.021.7948881
AGAGTAC421800.021.31307210-11
Produced by FastQC (version 0.11.5)