##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211371_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10331823 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738538978068053 31.0 31.0 34.0 30.0 34.0 2 31.82010619035963 33.0 31.0 34.0 30.0 34.0 3 31.892287062989755 33.0 31.0 34.0 30.0 34.0 4 35.521164367604825 37.0 35.0 37.0 33.0 37.0 5 35.56751494871718 37.0 35.0 37.0 33.0 37.0 6 35.633650518403186 37.0 35.0 37.0 33.0 37.0 7 35.573576802467485 37.0 35.0 37.0 33.0 37.0 8 35.577834424767055 37.0 35.0 37.0 33.0 37.0 9 37.21461275517399 39.0 37.0 39.0 34.0 39.0 10-11 37.14689411539474 39.0 37.0 39.0 33.5 39.0 12-13 37.07053764858341 39.0 37.0 39.0 33.0 39.0 14-15 38.330723338949966 40.0 38.0 41.0 33.0 41.0 16-17 38.32986274542257 40.0 38.0 41.0 33.0 41.0 18-19 38.32434295477188 40.0 38.0 41.0 33.5 41.0 20-21 38.30570790846882 40.0 38.0 41.0 34.0 41.0 22-23 38.186628729508826 40.0 38.0 41.0 33.5 41.0 24-25 38.10142842168318 40.0 38.0 41.0 33.0 41.0 26-27 37.974860244895794 40.0 38.0 41.0 33.0 41.0 28-29 37.858410756746416 40.0 37.5 41.0 33.0 41.0 30-31 37.70779967872079 40.0 37.0 41.0 33.0 41.0 32-33 37.5296926786299 40.0 37.0 41.0 32.0 41.0 34-35 37.41101134814253 40.0 37.0 41.0 32.0 41.0 36-37 37.285301538750716 40.0 36.0 41.0 31.5 41.0 38-39 37.14279880714178 39.5 36.0 41.0 31.0 41.0 40-41 36.9853121757893 39.0 36.0 41.0 31.0 41.0 42-43 36.79648538307325 39.0 36.0 41.0 30.0 41.0 44-45 36.662386541077986 39.0 35.0 41.0 30.0 41.0 46-47 36.44602303001126 39.0 35.0 40.5 29.5 41.0 48-49 36.207977140142646 39.0 35.0 40.0 29.0 41.0 50-51 35.55211011648186 38.0 34.5 39.5 28.5 40.5 52-53 35.8620316569496 38.5 35.0 40.0 28.5 40.5 54-55 36.263930237674415 39.0 35.0 40.5 29.0 41.0 56-57 36.179552001616756 39.0 35.0 41.0 29.0 41.0 58-59 35.97509815063614 38.5 35.0 40.0 28.5 41.0 60-61 35.748912074858424 38.0 35.0 40.0 28.0 41.0 62-63 35.473701494886235 38.0 34.0 40.0 28.0 41.0 64-65 35.26199209955494 37.0 34.0 40.0 28.0 41.0 66-67 35.064196996019 37.0 34.0 40.0 28.0 41.0 68-69 34.731816156742134 36.0 34.0 39.5 27.0 41.0 70-71 34.37286914419653 36.0 34.0 39.0 26.0 41.0 72-73 33.99538067967289 35.0 33.5 39.0 26.0 40.5 74-75 33.616293900892416 35.0 33.0 37.5 26.0 40.0 76-77 33.24439830221637 35.0 33.0 37.0 25.0 39.0 78-79 32.87844173288683 35.0 33.0 37.0 25.0 39.0 80-81 32.51369201737196 35.0 32.0 36.0 24.0 39.0 82-83 32.17478149790216 35.0 32.0 36.0 23.5 37.0 84-85 31.866245192160182 35.0 32.0 35.0 23.0 37.0 86-87 31.570801832358143 35.0 31.0 35.0 20.5 36.5 88-89 31.30459852051279 34.5 31.0 35.0 20.0 36.0 90-91 31.05249954436889 34.0 31.0 35.0 19.5 36.0 92-93 30.810418209835767 34.0 31.0 35.0 19.0 36.0 94-95 30.573687044387036 34.0 30.0 35.0 18.0 35.0 96-97 30.33208210206466 34.0 30.0 35.0 17.5 35.0 98-99 30.0798140850845 34.0 29.0 35.0 16.5 35.0 100 28.876184870762884 33.0 27.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2548913487434916 1101 2 -0.19689849081487765 1101 3 -0.29237969075864 1101 4 -0.18592498207330266 1101 5 -0.16484998987075983 1101 6 -0.2658094457905662 1101 7 -0.2500073855812843 1101 8 -0.22131910278061184 1101 9 -0.40509590858574995 1101 10-11 -0.33460751717602477 1101 12-13 -0.32654591028530433 1101 14-15 -0.5144794900474849 1101 16-17 -0.46339266528119083 1101 18-19 -0.49371281355748664 1101 20-21 -0.4935954843427339 1101 22-23 -0.503806399117579 1101 24-25 -0.5443747195435193 1101 26-27 -0.5479752659954684 1101 28-29 -0.5726808519493218 1101 30-31 -0.5928601181373736 1101 32-33 -0.613990270667351 1101 34-35 -0.6068504446952971 1101 36-37 -0.5939580026387574 1101 38-39 -0.6906487287415217 1101 40-41 -0.7143514342905775 1101 42-43 -0.7749986485041518 1101 44-45 -0.7927199314074684 1101 46-47 -0.8320483855543728 1101 48-49 -0.9312289263607312 1101 50-51 -0.7763857127562588 1101 52-53 -0.6826831887800111 1101 54-55 -0.6242880666795401 1101 56-57 -0.6808849474687477 1101 58-59 -0.62988422388473 1101 60-61 -0.6383367283973556 1101 62-63 -0.6518092688732366 1101 64-65 -0.605962028321251 1101 66-67 -0.6229798443241776 1101 68-69 -0.7183070348376432 1101 70-71 -0.7290431352946669 1101 72-73 -0.7418429867167191 1101 74-75 -0.8195038970760464 1101 76-77 -0.7683702791867759 1101 78-79 -0.8345834675065369 1101 80-81 -0.9203463686112627 1101 82-83 -0.9123225449153125 1101 84-85 -0.9643604424519978 1101 86-87 -0.9492672918454907 1101 88-89 -1.1190933933098712 1101 90-91 -1.2483144087158955 1101 92-93 -1.3062296182920718 1101 94-95 -1.347451003993779 1101 96-97 -1.490143263183949 1101 98-99 -1.4688176190644562 1101 100 -1.44859244794322 1102 1 -0.35032136242478273 1102 2 -0.3168782974061699 1102 3 -0.37518563224124435 1102 4 -0.28423107168610784 1102 5 -0.177480995077417 1102 6 -0.17560936362331603 1102 7 -0.27834822441211315 1102 8 -0.27224536667498 1102 9 -0.39743693401153735 1102 10-11 -0.3800060517275412 1102 12-13 -0.460702858854539 1102 14-15 -0.6329172635534803 1102 16-17 -0.6418362014847077 1102 18-19 -0.5951270770347108 1102 20-21 -0.6607497803641706 1102 22-23 -0.6152766282201725 1102 24-25 -0.6280138788093268 1102 26-27 -0.7045649088531931 1102 28-29 -0.7300802420784507 1102 30-31 -0.7076843856761812 1102 32-33 -0.7815765351743522 1102 34-35 -0.8393404432759581 1102 36-37 -0.792927737779145 1102 38-39 -0.8404885505363282 1102 40-41 -0.9780809939007682 1102 42-43 -0.929106905845245 1102 44-45 -0.8945808360936951 1102 46-47 -0.9776221217769177 1102 48-49 -0.9818475482564608 1102 50-51 -0.7979648812769895 1102 52-53 -0.6573688885216029 1102 54-55 -0.7139006787212736 1102 56-57 -0.6662316551551299 1102 58-59 -0.6508649381973299 1102 60-61 -0.7334180106086592 1102 62-63 -0.6971599972365823 1102 64-65 -0.816461595388219 1102 66-67 -0.8422893412824237 1102 68-69 -0.776479554732326 1102 70-71 -0.7832917655871654 1102 72-73 -0.7372733686775206 1102 74-75 -0.827300122994366 1102 76-77 -0.839858026702764 1102 78-79 -0.8861205960440373 1102 80-81 -0.9385929524388175 1102 82-83 -1.0167350186710138 1102 84-85 -1.0434237989210793 1102 86-87 -1.0524457603311674 1102 88-89 -1.0900639770130276 1102 90-91 -1.1332639415621024 1102 92-93 -1.252575870480996 1102 94-95 -1.304645776856514 1102 96-97 -1.3056077408127784 1102 98-99 -1.483428845673739 1102 100 -1.4300079296656811 1103 1 -0.36125100909313446 1103 2 -0.41399322049014486 1103 3 -0.42961883847081594 1103 4 -0.3145508358524154 1103 5 -0.20075706221388145 1103 6 -0.22992316002829227 1103 7 -0.2644232832533433 1103 8 -0.30068775611781007 1103 9 -0.3902685791494491 1103 10-11 -0.46663716169104674 1103 12-13 -0.45729008888455525 1103 14-15 -0.5962941597433726 1103 16-17 -0.636366036008198 1103 18-19 -0.6581259495838054 1103 20-21 -0.6446449156634557 1103 22-23 -0.6422714265267686 1103 24-25 -0.6336784676728726 1103 26-27 -0.715061277184823 1103 28-29 -0.6689665809638186 1103 30-31 -0.7415999999721876 1103 32-33 -0.7471525819118412 1103 34-35 -0.6405401590080899 1103 36-37 -0.7850304393593532 1103 38-39 -0.7707165357560513 1103 40-41 -0.8166335336117072 1103 42-43 -0.8165601103257956 1103 44-45 -0.8819681674879121 1103 46-47 -0.8616504246823098 1103 48-49 -0.9033069834164493 1103 50-51 -0.8321492971134958 1103 52-53 -0.6503827901409025 1103 54-55 -0.6534093418875813 1103 56-57 -0.6168362774120055 1103 58-59 -0.585507415674904 1103 60-61 -0.5661197160181786 1103 62-63 -0.5934607425289755 1103 64-65 -0.6165278473665765 1103 66-67 -0.6566857959261299 1103 68-69 -0.6779856529172363 1103 70-71 -0.7237658856610167 1103 72-73 -0.7308347104504662 1103 74-75 -0.7876507543885225 1103 76-77 -0.7964636045206461 1103 78-79 -0.8111995915028878 1103 80-81 -0.7458950185347177 1103 82-83 -0.7524023214369997 1103 84-85 -0.8214303683911943 1103 86-87 -0.9223510274387543 1103 88-89 -0.9117981230172134 1103 90-91 -0.8913829035880845 1103 92-93 -0.9931393996611924 1103 94-95 -1.0607557638951128 1103 96-97 -1.171542254714069 1103 98-99 -1.220347986536762 1103 100 -1.2319027240092062 1104 1 -0.45316526759398457 1104 2 -0.42399323796146504 1104 3 -0.4523630985162228 1104 4 -0.2440145564892049 1104 5 -0.2664366685028625 1104 6 -0.28174536450006826 1104 7 -0.3149651232185917 1104 8 -0.3747972747626136 1104 9 -0.42865089915482457 1104 10-11 -0.4764775998944444 1104 12-13 -0.4827650219540516 1104 14-15 -0.6447222768029235 1104 16-17 -0.6563291332729762 1104 18-19 -0.6773496440192801 1104 20-21 -0.6240311415478956 1104 22-23 -0.6739038905642971 1104 24-25 -0.6812607957245049 1104 26-27 -0.6913255078708502 1104 28-29 -0.8408773669215819 1104 30-31 -0.7567164937821715 1104 32-33 -0.7533249635026706 1104 34-35 -0.831642305652629 1104 36-37 -0.8903645611372042 1104 38-39 -0.8455341741217168 1104 40-41 -0.907154159143559 1104 42-43 -0.8825981265980118 1104 44-45 -0.8974762549859889 1104 46-47 -0.9142446490508149 1104 48-49 -0.8836787452664794 1104 50-51 -0.7897951910248935 1104 52-53 -0.7045236750009778 1104 54-55 -0.7311314811157814 1104 56-57 -0.6452014745784638 1104 58-59 -0.6799166260264471 1104 60-61 -0.6769889529553978 1104 62-63 -0.7140754822891608 1104 64-65 -0.7114738994689844 1104 66-67 -0.6966723255376834 1104 68-69 -0.6744114810448636 1104 70-71 -0.7388867945210222 1104 72-73 -0.8063938883407644 1104 74-75 -0.7641624875846418 1104 76-77 -0.824221472600172 1104 78-79 -0.7862650770830726 1104 80-81 -0.7938651508865853 1104 82-83 -0.7967755691992693 1104 84-85 -0.8951677360949262 1104 86-87 -0.8901155195099157 1104 88-89 -0.8993520497839533 1104 90-91 -0.8851753314514781 1104 92-93 -0.9500152687118444 1104 94-95 -0.9960032000613985 1104 96-97 -1.0873112338926454 1104 98-99 -1.0413743128425779 1104 100 -1.0897325177394848 1105 1 -0.4237587803977583 1105 2 -0.3963424050046278 1105 3 -0.4113084969137155 1105 4 -0.2593352788194423 1105 5 -0.2636343081817927 1105 6 -0.2590721902877391 1105 7 -0.29431107732519024 1105 8 -0.27457362176983935 1105 9 -0.4301752979857909 1105 10-11 -0.3942913535345056 1105 12-13 -0.4529655448262204 1105 14-15 -0.5829432497151927 1105 16-17 -0.5988802887142128 1105 18-19 -0.5417751246602549 1105 20-21 -0.649350517426214 1105 22-23 -0.5723152032335577 1105 24-25 -0.6165933544663247 1105 26-27 -0.6909135119001348 1105 28-29 -0.6934807019547691 1105 30-31 -0.6571305391992794 1105 32-33 -0.7300774748759622 1105 34-35 -0.7336331996990069 1105 36-37 -0.7387161421763224 1105 38-39 -0.7770983276328138 1105 40-41 -0.7206103661381036 1105 42-43 -0.7665847257375233 1105 44-45 -0.7875013131891251 1105 46-47 -0.8540812841300536 1105 48-49 -0.8777225731926706 1105 50-51 -0.8039957706861927 1105 52-53 -0.6639076892733655 1105 54-55 -0.5638135332092702 1105 56-57 -0.5570582775840762 1105 58-59 -0.559353488536992 1105 60-61 -0.6262993772182881 1105 62-63 -0.7299290721292238 1105 64-65 -0.6598149126923758 1105 66-67 -0.6373097102334171 1105 68-69 -0.624881537030241 1105 70-71 -0.6261319218191232 1105 72-73 -0.5684617499758815 1105 74-75 -0.5468750523855093 1105 76-77 -0.6724029381398182 1105 78-79 -0.6634869116787812 1105 80-81 -0.6868492547392435 1105 82-83 -0.7465458709121222 1105 84-85 -0.6241851147133168 1105 86-87 -0.7157026401018598 1105 88-89 -0.7677967237765664 1105 90-91 -0.8823376267673133 1105 92-93 -0.7639496722302077 1105 94-95 -0.8465735300772259 1105 96-97 -0.8403231529690522 1105 98-99 -0.9282667865197887 1105 100 -0.9585659339284618 1106 1 -0.4138848840711553 1106 2 -0.4634047750069499 1106 3 -0.4321161215929976 1106 4 -0.2980787385791146 1106 5 -0.29810385742949563 1106 6 -0.3241603297040214 1106 7 -0.3745286130481702 1106 8 -0.35183535974269375 1106 9 -0.5453371243606924 1106 10-11 -0.5732064339288385 1106 12-13 -0.5220947796516953 1106 14-15 -0.6651241093506499 1106 16-17 -0.62994158611545 1106 18-19 -0.6336156838135096 1106 20-21 -0.6971752828686064 1106 22-23 -0.6439048129876568 1106 24-25 -0.6480281496768114 1106 26-27 -0.6977757723227214 1106 28-29 -0.7322517127747901 1106 30-31 -0.7225864833196383 1106 32-33 -0.8350014811224256 1106 34-35 -0.7998730358892416 1106 36-37 -0.7559477217470274 1106 38-39 -0.8638454869629157 1106 40-41 -0.8730895210649408 1106 42-43 -0.84487567229516 1106 44-45 -0.8973418371020045 1106 46-47 -0.9044124154230957 1106 48-49 -0.8491117588801274 1106 50-51 -0.9076535433583288 1106 52-53 -0.7818203337324192 1106 54-55 -0.7528623742525298 1106 56-57 -0.8231710198017197 1106 58-59 -0.725579591017123 1106 60-61 -0.6102037477721822 1106 62-63 -0.7349184180488706 1106 64-65 -0.7387139899409547 1106 66-67 -0.710498201529326 1106 68-69 -0.677364422307086 1106 70-71 -0.7021476169461494 1106 72-73 -0.6454931151378958 1106 74-75 -0.6961942551455849 1106 76-77 -0.7401584116338711 1106 78-79 -0.7314566433177419 1106 80-81 -0.678750525478236 1106 82-83 -0.7205695591364929 1106 84-85 -0.7194238721688571 1106 86-87 -0.6683818343507184 1106 88-89 -0.7168811319506823 1106 90-91 -0.7728944157980102 1106 92-93 -0.9046235295693528 1106 94-95 -0.8161735138844932 1106 96-97 -0.7843037397592418 1106 98-99 -0.8696421559722012 1106 100 -0.8891164856355189 1107 1 -0.4292086184046653 1107 2 -0.44954925193259854 1107 3 -0.4580577778958279 1107 4 -0.302134542060962 1107 5 -0.30902850910731416 1107 6 -0.3609036006424162 1107 7 -0.4016712023395854 1107 8 -0.3548911368759491 1107 9 -0.4242703895768116 1107 10-11 -0.4567007426494527 1107 12-13 -0.4960380997081728 1107 14-15 -0.617646161511793 1107 16-17 -0.6605751934664426 1107 18-19 -0.7416376890759082 1107 20-21 -0.6917912388394356 1107 22-23 -0.6791191126158651 1107 24-25 -0.715057676137647 1107 26-27 -0.7089425133689318 1107 28-29 -0.7094596475636834 1107 30-31 -0.7534851210116926 1107 32-33 -0.7776678407955657 1107 34-35 -0.7651706980876085 1107 36-37 -0.7221324312248925 1107 38-39 -0.7415322230454251 1107 40-41 -0.7569796192739631 1107 42-43 -0.7772922867785326 1107 44-45 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0.7213109933135833 2206 90-91 0.7806750313865756 2206 92-93 0.7755133224187638 2206 94-95 0.719499374812802 2206 96-97 0.7313936807895054 2206 98-99 0.8084803440827137 2206 100 0.8778726433253539 2207 1 0.5572403247282516 2207 2 0.5549977377809903 2207 3 0.6335211419435289 2207 4 0.46891829771987403 2207 5 0.4938587831544865 2207 6 0.49728949649059473 2207 7 0.5356033380303771 2207 8 0.5174746146976545 2207 9 0.6845514894954334 2207 10-11 0.6744603958061717 2207 12-13 0.7088195908043531 2207 14-15 0.8786414262576372 2207 16-17 0.9044833730943793 2207 18-19 0.8908191820489364 2207 20-21 0.8475921278528347 2207 22-23 0.8898000896504143 2207 24-25 0.9048130563005401 2207 26-27 0.9425604914191794 2207 28-29 0.934739957413619 2207 30-31 0.9245667253246879 2207 32-33 0.9486540905227727 2207 34-35 0.9866763005513235 2207 36-37 1.0590448988735588 2207 38-39 1.0121346037299617 2207 40-41 0.9787914086296041 2207 42-43 1.0411705203134005 2207 44-45 1.0461258436740053 2207 46-47 1.0712663172528636 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0.9026208746308058 2209 34-35 0.9181201253702014 2209 36-37 0.9220053532580224 2209 38-39 0.9016252893633734 2209 40-41 0.960400388801375 2209 42-43 1.0515384302056603 2209 44-45 1.0892777009640753 2209 46-47 1.0185883657417847 2209 48-49 0.9647269164061285 2209 50-51 0.9737835281649652 2209 52-53 0.9228915355831688 2209 54-55 0.8840062122133787 2209 56-57 0.9265196661584696 2209 58-59 0.9240231450580723 2209 60-61 0.9330670599284758 2209 62-63 0.9343028397778141 2209 64-65 0.887865956493691 2209 66-67 0.8638724489850418 2209 68-69 0.8686647527278595 2209 70-71 0.8421785694098531 2209 72-73 0.8375849025750028 2209 74-75 0.8803394640901558 2209 76-77 0.8385253785369216 2209 78-79 0.852562403309733 2209 80-81 0.7828361246530804 2209 82-83 0.8423436862424651 2209 84-85 0.8492101710440885 2209 86-87 0.9753873538132858 2209 88-89 0.9071477919961559 2209 90-91 0.9214242197799827 2209 92-93 0.9322456674805615 2209 94-95 0.9627979819742905 2209 96-97 0.9285758594460951 2209 98-99 1.0347765323639102 2209 100 1.1263560459118338 2210 1 0.5577391766853914 2210 2 0.5782821505439948 2210 3 0.6440038666319161 2210 4 0.48560889184988554 2210 5 0.4661197620571187 2210 6 0.5367784272657516 2210 7 0.5404103218389267 2210 8 0.5554432205980717 2210 9 0.7080459608252596 2210 10-11 0.7197987297416475 2210 12-13 0.7971403047776775 2210 14-15 0.9532625780559982 2210 16-17 0.912170565880011 2210 18-19 0.8974566762255307 2210 20-21 0.957274342220721 2210 22-23 0.948457830264033 2210 24-25 0.9199317765298645 2210 26-27 0.9682501787617142 2210 28-29 0.9826050849580881 2210 30-31 1.0074449317632386 2210 32-33 1.090427133383038 2210 34-35 1.1003135285150805 2210 36-37 1.0652104297780127 2210 38-39 1.0785594604028717 2210 40-41 1.0857207267444409 2210 42-43 1.1084408107230814 2210 44-45 1.1150550619590263 2210 46-47 1.2038385274849404 2210 48-49 1.19613881255993 2210 50-51 1.1366448240989158 2210 52-53 1.0841655750270505 2210 54-55 1.0292931819051887 2210 56-57 1.0162157695745577 2210 58-59 0.9884468670241162 2210 60-61 1.0005842778874552 2210 62-63 1.0168780905555792 2210 64-65 0.9938931373356965 2210 66-67 0.898147957722415 2210 68-69 0.9545746728131874 2210 70-71 0.9170149498669886 2210 72-73 0.8849439561559933 2210 74-75 0.9061980949540285 2210 76-77 0.9354804206649945 2210 78-79 0.952900478377984 2210 80-81 0.9681369828629016 2210 82-83 0.9961421242223025 2210 84-85 1.0730232238182786 2210 86-87 1.1156364451080485 2210 88-89 1.090512372661582 2210 90-91 1.0862176333537015 2210 92-93 1.0948680091371443 2210 94-95 1.099819006258624 2210 96-97 1.1446894788068818 2210 98-99 1.162843406231758 2210 100 1.2924414891892475 2211 1 0.5583829658868602 2211 2 0.5358615620232392 2211 3 0.5845909198032793 2211 4 0.4147699180731266 2211 5 0.41426768562969585 2211 6 0.4295910457201586 2211 7 0.43226519911736005 2211 8 0.47013178146276147 2211 9 0.6059255670004831 2211 10-11 0.6182474937428495 2211 12-13 0.642179339169445 2211 14-15 0.8111974999553553 2211 16-17 0.8377452908720713 2211 18-19 0.7785186363818255 2211 20-21 0.7626810991151132 2211 22-23 0.8480084528486884 2211 24-25 0.8728398310066083 2211 26-27 0.8069542598684833 2211 28-29 0.8394654711630452 2211 30-31 0.8476017966238132 2211 32-33 0.9047203349741721 2211 34-35 0.9346535777737657 2211 36-37 0.9429103077986483 2211 38-39 1.011435685880329 2211 40-41 0.9648737995091707 2211 42-43 0.9985651480920552 2211 44-45 1.0471988262844292 2211 46-47 1.0841334459577254 2211 48-49 1.0770007797457524 2211 50-51 1.073321629672904 2211 52-53 0.9663044826579466 2211 54-55 0.9360224921572069 2211 56-57 0.8618390824116489 2211 58-59 0.8492890864134566 2211 60-61 0.8266382627931819 2211 62-63 0.8478482265613749 2211 64-65 0.7943504784046596 2211 66-67 0.8175626233187998 2211 68-69 0.7897513453665042 2211 70-71 0.7984642858902049 2211 72-73 0.793398498489438 2211 74-75 0.7911768148328377 2211 76-77 0.7718694731428855 2211 78-79 0.774054359920612 2211 80-81 0.7704920954699048 2211 82-83 0.8146685990313145 2211 84-85 0.7788767596499007 2211 86-87 0.8162160516451884 2211 88-89 0.8672337153093714 2211 90-91 0.8574517459348101 2211 92-93 0.954707686223415 2211 94-95 0.9530565991182165 2211 96-97 0.914383050057328 2211 98-99 0.9578031054801528 2211 100 1.029478256622319 2212 1 0.5012899183162354 2212 2 0.5012062350952711 2212 3 0.565652177235787 2212 4 0.42907939004604856 2212 5 0.4220069050997495 2212 6 0.4655038541684533 2212 7 0.4822366023204765 2212 8 0.4614453236126863 2212 9 0.6341854779357519 2212 10-11 0.649851325671257 2212 12-13 0.6379268173936978 2212 14-15 0.7997278913921733 2212 16-17 0.815355097975015 2212 18-19 0.8226143333640081 2212 20-21 0.8078742273124746 2212 22-23 0.8270751203186961 2212 24-25 0.8973796248852537 2212 26-27 0.8757820819456086 2212 28-29 0.8748019124184836 2212 30-31 0.8691610969247492 2212 32-33 0.924393514333083 2212 34-35 0.9881786081287842 2212 36-37 0.9137150887858425 2212 38-39 0.9603959079670972 2212 40-41 0.9995545163318553 2212 42-43 1.030428692111208 2212 44-45 1.035368775184594 2212 46-47 1.0643676063806993 2212 48-49 1.0690451842026505 2212 50-51 0.9920743074269964 2212 52-53 0.9360792685799524 2212 54-55 0.8827193838537042 2212 56-57 0.857748075742677 2212 58-59 0.8398639908314607 2212 60-61 0.8333684642627333 2212 62-63 0.8255068235345817 2212 64-65 0.7868150137610215 2212 66-67 0.8006837050550715 2212 68-69 0.776617934054137 2212 70-71 0.8082031161836483 2212 72-73 0.8218941319342434 2212 74-75 0.7862625343786718 2212 76-77 0.7741348763656433 2212 78-79 0.8151267616375435 2212 80-81 0.8101404152107037 2212 82-83 0.8222631347943192 2212 84-85 0.8492316328081237 2212 86-87 0.8563121032014855 2212 88-89 0.8066409342313072 2212 90-91 0.8704195149006253 2212 92-93 0.9314654025634219 2212 94-95 0.9481472842494192 2212 96-97 0.9088869577703313 2212 98-99 0.9428064020208033 2212 100 0.9529151580631527 2213 1 0.4115038093942651 2213 2 0.41576567160812417 2213 3 0.45484705611337617 2213 4 0.2978989962321279 2213 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68-69 0.740889595275128 2213 70-71 0.7219435811344468 2213 72-73 0.7499595112332145 2213 74-75 0.7514702169660623 2213 76-77 0.7437746625281321 2213 78-79 0.7668889042013376 2213 80-81 0.7193093450806671 2213 82-83 0.8243810231149311 2213 84-85 0.7894124135121494 2213 86-87 0.7707170883183316 2213 88-89 0.8353883817402625 2213 90-91 0.8762460322891528 2213 92-93 0.9414934462158016 2213 94-95 0.9346555772907514 2213 96-97 0.9170776699286556 2213 98-99 0.9654039092241717 2213 100 1.0300214211562917 2214 1 0.4619604180373962 2214 2 0.4303749159678354 2214 3 0.4455018043772405 2214 4 0.33476857412664884 2214 5 0.30934269226305844 2214 6 0.34179541172041894 2214 7 0.3188985913651692 2214 8 0.32691899272418823 2214 9 0.46397405077216547 2214 10-11 0.500066721852896 2214 12-13 0.5650058393439892 2214 14-15 0.7490458964014195 2214 16-17 0.7176874255207863 2214 18-19 0.7272734020408294 2214 20-21 0.7316203413306681 2214 22-23 0.7551721758714294 2214 24-25 0.7944008063015957 2214 26-27 0.8209503554888613 2214 28-29 0.8657246364945266 2214 30-31 0.8579481819557131 2214 32-33 0.8365502783749506 2214 34-35 0.8513238370126004 2214 36-37 0.8381372222899373 2214 38-39 0.8596958676145974 2214 40-41 0.9707842745144575 2214 42-43 1.050859555663365 2214 44-45 1.0618583007854099 2214 46-47 1.046946284901452 2214 48-49 1.043202951708885 2214 50-51 0.9657783295362989 2214 52-53 0.8431407374586044 2214 54-55 0.8123319290849906 2214 56-57 0.7581694929147957 2214 58-59 0.7821305776113192 2214 60-61 0.7937278232593314 2214 62-63 0.8236403225898599 2214 64-65 0.8009544449986734 2214 66-67 0.7593510624209472 2214 68-69 0.7975515689583048 2214 70-71 0.7851701742355743 2214 72-73 0.7867353942945954 2214 74-75 0.768866839696031 2214 76-77 0.7983489890813473 2214 78-79 0.7542247213306581 2214 80-81 0.7878087970452512 2214 82-83 0.8701444123613769 2214 84-85 0.8694167633906282 2214 86-87 0.9041989289610548 2214 88-89 0.9643470908359753 2214 90-91 0.9825765070812977 2214 92-93 1.0455044174889458 2214 94-95 1.025241037576503 2214 96-97 1.0395078359268943 2214 98-99 1.0680922989173887 2214 100 1.1953156587406433 2215 1 0.3996868571785477 2215 2 0.38011118685386336 2215 3 0.39854799450006695 2215 4 0.3185439810913877 2215 5 0.28539010089772887 2215 6 0.3192595012074122 2215 7 0.3516975606899848 2215 8 0.3422175797355038 2215 9 0.47126694541185543 2215 10-11 0.48291189598660367 2215 12-13 0.500801202394122 2215 14-15 0.6486107454139116 2215 16-17 0.5700502624725203 2215 18-19 0.5851869347510572 2215 20-21 0.6007274620391954 2215 22-23 0.7000060420269278 2215 24-25 0.6698223966322558 2215 26-27 0.6795509179739057 2215 28-29 0.7488209919325044 2215 30-31 0.7547569970828363 2215 32-33 0.769130675673388 2215 34-35 0.8393769583506767 2215 36-37 0.836943810847643 2215 38-39 0.8429155542188838 2215 40-41 0.9002395346923606 2215 42-43 0.9132346029986635 2215 44-45 0.9549548327794 2215 46-47 0.9979928001292109 2215 48-49 1.079435998238587 2215 50-51 1.010546269475121 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0.9352177693522634 2311 28-29 0.9277806077135509 2311 30-31 0.9424637909402591 2311 32-33 0.9299021541032673 2311 34-35 0.9509937710668126 2311 36-37 0.9972532790281861 2311 38-39 1.010735336847162 2311 40-41 1.035249714215766 2311 42-43 1.0696340008323375 2311 44-45 1.0662866412423995 2311 46-47 1.1262273862920367 2311 48-49 1.1277749170979092 2311 50-51 1.1050618894490114 2311 52-53 0.9333120003566435 2311 54-55 0.8970930485968367 2311 56-57 0.8805502876262636 2311 58-59 0.8633697681886048 2311 60-61 0.8434468951407013 2311 62-63 0.8692640920047623 2311 64-65 0.8264660478048214 2311 66-67 0.8574002784755024 2311 68-69 0.8687156086134635 2311 70-71 0.821203334178719 2311 72-73 0.8430591491431159 2311 74-75 0.8493477302031422 2311 76-77 0.8602215464980816 2311 78-79 0.9034763012591469 2311 80-81 0.9234088696839109 2311 82-83 0.9409987480562236 2311 84-85 0.8974206251353571 2311 86-87 0.9389636085277537 2311 88-89 0.9550984994773977 2311 90-91 1.0404582992324798 2311 92-93 1.0342151980923369 2311 94-95 1.055942064095536 2311 96-97 1.128579075733807 2311 98-99 1.184845728188911 2311 100 1.3109874289767838 2312 1 0.4603241544634038 2312 2 0.49828113184202394 2312 3 0.5517486002821279 2312 4 0.396891145342245 2312 5 0.3972081703938031 2312 6 0.24203538787242707 2312 7 0.38265435511254253 2312 8 0.44846218922387493 2312 9 0.5898484179486019 2312 10-11 0.6191353032496849 2312 12-13 0.6617193217561095 2312 14-15 0.8818206251734253 2312 16-17 0.8787565967050881 2312 18-19 0.872274373657163 2312 20-21 0.883397610579344 2312 22-23 0.9134536146311234 2312 24-25 0.8981465423591075 2312 26-27 0.9137451972533555 2312 28-29 0.9961144737328524 2312 30-31 0.8696827325158623 2312 32-33 0.9303999893573263 2312 34-35 0.9956843765573709 2312 36-37 1.054366211800719 2312 38-39 1.1112080733674503 2312 40-41 1.1081492286578367 2312 42-43 1.11701583333614 2312 44-45 1.1356226218076415 2312 46-47 1.1487652245076774 2312 48-49 1.151365942827347 2312 50-51 1.0710248593538054 2312 52-53 0.9386925275205869 2312 54-55 0.9019825242056712 2312 56-57 0.860725236968193 2312 58-59 0.8560687749014377 2312 60-61 0.8382664728107798 2312 62-63 0.8695493755911201 2312 64-65 0.8688132900467664 2312 66-67 0.8844391660154045 2312 68-69 0.9137954149428893 2312 70-71 0.9754666353299157 2312 72-73 1.0002137250023253 2312 74-75 0.9638449817987791 2312 76-77 0.9171804921615561 2312 78-79 0.9490749637384539 2312 80-81 0.8087095609964834 2312 82-83 0.9271446571480126 2312 84-85 1.0150146749787403 2312 86-87 1.0471185999924835 2312 88-89 1.061100690272493 2312 90-91 1.0995094335171025 2312 92-93 1.163791695036931 2312 94-95 1.189461524943777 2312 96-97 1.2704333440022673 2312 98-99 1.154837021690657 2312 100 1.2496762690422294 2313 1 0.4684951054000841 2313 2 0.4688012820047369 2313 3 0.5074393724063277 2313 4 0.3447904448928796 2313 5 0.3167888331231694 2313 6 0.3772755965579151 2313 7 0.4305709936885407 2313 8 0.42921342584414646 2313 9 0.5789643221364358 2313 10-11 0.4738758333472006 2313 12-13 0.6019448357408166 2313 14-15 0.7543055139935788 2313 16-17 0.7473649193437524 2313 18-19 0.7424675313600702 2313 20-21 0.7762658183548226 2313 22-23 0.8230239282837388 2313 24-25 0.8767035380202017 2313 26-27 0.8804978056797879 2313 28-29 0.8829552061350014 2313 30-31 0.8293177569629293 2313 32-33 0.8885096319720986 2313 34-35 0.9403453657557819 2313 36-37 0.957896779696199 2313 38-39 0.9510728197447449 2313 40-41 0.958198232476434 2313 42-43 0.9973034257325395 2313 44-45 0.9451033483897646 2313 46-47 0.9887574704086006 2313 48-49 1.020664600923773 2313 50-51 0.9801701956557665 2313 52-53 0.8512125036991094 2313 54-55 0.8026695507035981 2313 56-57 0.7665962257737249 2313 58-59 0.8209261429364645 2313 60-61 0.8136675061691747 2313 62-63 0.8409808955584452 2313 64-65 0.8358872346778199 2313 66-67 0.8019021544750942 2313 68-69 0.8392538726865624 2313 70-71 0.7987272683030326 2313 72-73 0.8628030326404286 2313 74-75 0.8239740616871813 2313 76-77 0.8261154533017887 2313 78-79 0.8704115076495214 2313 80-81 0.9033787312932233 2313 82-83 0.9158378207080915 2313 84-85 0.9421015428134005 2313 86-87 0.9767265925691895 2313 88-89 1.0253228005715194 2313 90-91 1.032402094739549 2313 92-93 1.1171293019852975 2313 94-95 1.1504926372664563 2313 96-97 1.1600986783192333 2313 98-99 1.2484700490600993 2313 100 1.4045577733392207 2314 1 0.36455367315605613 2314 2 0.3808259527203681 2314 3 0.4306346777273937 2314 4 0.3286290387126485 2314 5 0.3700119112816722 2314 6 0.36620378392837694 2314 7 0.4171735044443068 2314 8 0.41912393186693464 2314 9 0.5058847841452803 2314 10-11 0.4846954137091686 2314 12-13 0.24147217053195646 2314 14-15 0.6411758586064309 2314 16-17 0.6673758877638107 2314 18-19 0.7072685701617942 2314 20-21 0.7058170959311596 2314 22-23 0.5786700968982004 2314 24-25 0.6071296860624713 2314 26-27 0.6984028878341277 2314 28-29 0.7866658675367546 2314 30-31 0.7343802707174234 2314 32-33 0.7502340832743712 2314 34-35 0.8293548769043539 2314 36-37 0.8765665614900868 2314 38-39 0.9188573145108165 2314 40-41 0.9480512489086763 2314 42-43 1.0039250309415735 2314 44-45 1.0411507015792978 2314 46-47 0.984987319323082 2314 48-49 0.9989003681553683 2314 50-51 0.9705649250485422 2314 52-53 0.7963786883157908 2314 54-55 0.7509885709070261 2314 56-57 0.7791765441923815 2314 58-59 0.7345472754964177 2314 60-61 0.7504562938547679 2314 62-63 0.773047562934309 2314 64-65 0.7161263278051209 2314 66-67 0.7489002692563318 2314 68-69 0.7401312637504205 2314 70-71 0.7946756720084309 2314 72-73 0.8047826936203677 2314 74-75 0.792550684534767 2314 76-77 0.8617957211957616 2314 78-79 0.8560463416426742 2314 80-81 0.904861587596109 2314 82-83 0.7841867938936744 2314 84-85 0.7932251674051223 2314 86-87 0.9749162186911597 2314 88-89 1.0930247827144122 2314 90-91 1.0826819313119138 2314 92-93 1.1745988854305587 2314 94-95 1.2019406095904834 2314 96-97 1.2448955594895459 2314 98-99 1.3103594227397437 2314 100 1.2041652056117194 2315 1 0.3351375101714247 2315 2 0.37786858065097206 2315 3 0.4315434968474712 2315 4 0.3080273343999522 2315 5 0.30831583484159353 2315 6 -0.422951651471422 2315 7 0.1470911644814663 2315 8 0.17296367524239997 2315 9 0.4033769380310943 2315 10-11 0.44797073267088905 2315 12-13 0.5089406043023175 2315 14-15 0.6322738298645945 2315 16-17 0.5258147511495395 2315 18-19 0.629337568256723 2315 20-21 0.6457354607791714 2315 22-23 0.5636180015916636 2315 24-25 0.6713884168789619 2315 26-27 0.7357819725243999 2315 28-29 0.7927067038576538 2315 30-31 0.8518035032921887 2315 32-33 0.8765382595998901 2315 34-35 0.8603015256770661 2315 36-37 0.8743422780817482 2315 38-39 0.9764050241543458 2315 40-41 0.9975785125105219 2315 42-43 0.9792941134933244 2315 44-45 0.9991956168061051 2315 46-47 1.0660206938578725 2315 48-49 1.05390483432884 2315 50-51 0.9943753230206269 2315 52-53 0.7276433823068231 2315 54-55 0.599600959621938 2315 56-57 0.6067151972494926 2315 58-59 0.5333564793587087 2315 60-61 0.4757334243517448 2315 62-63 0.6872976499640089 2315 64-65 0.7776245867660876 2315 66-67 0.7748303279100242 2315 68-69 0.8103202746962737 2315 70-71 0.8261025863223352 2315 72-73 0.8710151188564907 2315 74-75 0.8793174208213586 2315 76-77 0.8891828329557612 2315 78-79 0.8055749578323201 2315 80-81 0.7939336826387802 2315 82-83 0.9144564349352251 2315 84-85 0.927195937304699 2315 86-87 0.9354776737690251 2315 88-89 1.0803771937557407 2315 90-91 1.195240437167147 2315 92-93 1.278698574202771 2315 94-95 1.3195376836028139 2315 96-97 1.3001507024427887 2315 98-99 1.4228422920548347 2315 100 1.5281824804949586 2316 1 0.07055757520437922 2316 2 -0.02743241197590507 2316 3 0.11887217772604686 2316 4 0.04769107439409481 2316 5 0.09732395992115528 2316 6 0.12783921348116678 2316 7 0.14240619718675163 2316 8 0.18022639781369776 2316 9 0.2739004026161709 2316 10-11 0.12586891169388537 2316 12-13 0.23262395351701315 2316 14-15 0.41304659190045356 2316 16-17 0.47560421333719205 2316 18-19 0.42002478233441565 2316 20-21 0.39368664398913467 2316 22-23 0.27562286266768865 2316 24-25 0.3309972189934527 2316 26-27 0.38813733425549657 2316 28-29 0.5278953417382866 2316 30-31 0.5157802160709295 2316 32-33 0.6006518520849227 2316 34-35 0.6305420934235642 2316 36-37 0.6603017878072492 2316 38-39 0.6965060855212002 2316 40-41 0.6728190430307848 2316 42-43 0.7280575688730977 2316 44-45 0.6716953429708994 2316 46-47 0.6450974682566937 2316 48-49 0.6549579699835135 2316 50-51 0.45802440167863523 2316 52-53 0.1846256140362712 2316 54-55 0.17321447339572416 2316 56-57 0.28187654434006504 2316 58-59 0.3374977815660145 2316 60-61 0.4000356374751419 2316 62-63 0.5093661166005461 2316 64-65 0.51413728138116 2316 66-67 0.5063531930857081 2316 68-69 0.5034339702815274 2316 70-71 0.3935189982858063 2316 72-73 0.544008720172485 2316 74-75 0.6060759463997556 2316 76-77 0.6449461577451743 2316 78-79 0.7302218761380175 2316 80-81 0.8136821878044103 2316 82-83 0.809069321760731 2316 84-85 0.8649815199460242 2316 86-87 0.9182920608032497 2316 88-89 1.0594820840144514 2316 90-91 1.017789402490461 2316 92-93 1.1093148148500127 2316 94-95 1.2178027168692331 2316 96-97 1.2445822402356228 2316 98-99 1.336003086198744 2316 100 1.214065047066768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 28.0 1 506.0 2 269.0 3 192.0 4 167.0 5 127.0 6 181.0 7 1138.0 8 3912.0 9 7209.0 10 9513.0 11 11258.0 12 12417.0 13 13677.0 14 15026.0 15 17093.0 16 21339.0 17 27747.0 18 36395.0 19 46083.0 20 58387.0 21 72244.0 22 80800.0 23 81836.0 24 79377.0 25 80929.0 26 89167.0 27 104826.0 28 129016.0 29 158506.0 30 199353.0 31 258776.0 32 341756.0 33 467627.0 34 679250.0 35 966024.0 36 1463164.0 37 2151393.0 38 2060027.0 39 584249.0 40 839.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.000015517995536 19.362811705611986 12.668396923169434 23.96877585322304 2 15.607611727390072 20.51153469130301 40.68856517632199 23.19228840498493 3 18.08732138813573 27.096570766442476 30.17303630173569 24.64307154368611 4 12.12970962271903 16.926731694056574 39.2304597391805 31.713098944043892 5 12.811828111058787 37.973459031426586 35.058003184792305 14.156709672722315 6 29.626417469617007 37.84777029971433 18.886263496828548 13.63954873384012 7 26.380714244140187 32.809757929581615 23.634747032117716 17.17478079416049 8 25.775193929818734 35.0576983879894 20.88746042359763 18.279647258594235 9 26.919172959813487 15.29748583648608 20.91305489113864 36.870286312561795 10-11 24.688576174418547 25.922535848495365 29.15491227787334 20.23397569921275 12-13 25.337471007587165 24.019646384064917 28.794028913692514 21.848853694655404 14-15 22.738651545881964 25.262795529709724 26.70218462140454 25.296368303003764 16-17 21.959474225635965 27.78936033973852 27.430384548532665 22.820780886092855 18-19 21.610825366636032 27.471062896359438 29.234876150208034 21.6832355867965 20-21 22.706815656119385 26.72164683578928 28.70895540340887 21.862582104682463 22-23 22.551509307952884 26.586537607461867 28.564882082676572 22.297071001908677 24-25 22.15015426549503 26.59209343414823 28.976530085680142 22.2812222146766 26-27 22.125931348112733 26.685555366946062 28.718447862293704 22.470065422647494 28-29 21.910951930460676 27.319426727005585 28.666824943798307 22.10279639873543 30-31 22.62552398211158 26.775948746133587 28.59201187591949 22.006515395835343 32-33 22.555971268181565 26.95649484245649 28.301207104837662 22.186326784524287 34-35 22.609955545246237 26.936769383716097 28.65516407133478 21.798110999702885 36-37 22.41053367797221 26.828925559948225 28.630220196903817 22.130320565175747 38-39 22.380137480292124 26.93874879884325 28.690670891666358 21.99044282919827 40-41 22.34555042856022 26.510909960701497 28.89856976530923 22.244969845429054 42-43 22.176443807509596 26.716029386244 28.442627335267535 22.66489947097887 44-45 21.951526041528997 27.10350471400198 28.580083909264285 22.36488533520474 46-47 21.994171981872327 26.74635146108828 28.358335049217263 22.901141507822132 48-49 22.17392617807399 26.743157556560888 28.394652789887697 22.68826347547743 50-51 22.061205376857984 27.028620801141944 28.149610368084904 22.760563453915168 52-53 21.84433659331644 27.248936880462203 27.97552555638425 22.931200969837107 54-55 21.72695753169225 27.008889008309495 28.2522850689303 23.011868391067953 56-57 22.142876335854208 26.855032388269006 28.027773615452638 22.97431766042415 58-59 21.94650943780359 26.90571802192704 28.297612551195943 22.850159989073422 60-61 22.03368843821796 27.13954682413709 28.030214176742973 22.79655056090198 62-63 22.01889401548486 26.82508540431713 27.974074979987655 23.181945600210355 64-65 21.6635031438053 26.728284527691493 28.066451793662278 23.54176053484093 66-67 21.90886161884092 26.544110876952605 27.86675897892777 23.68026852527871 68-69 21.653295250313157 26.697391272775683 28.01027757438594 23.63903590252522 70-71 21.905780731405258 26.365667966425637 28.038407076415673 23.690144225753432 72-73 22.07965594696285 26.576547854982014 27.868102351227584 23.475693846827554 74-75 22.118387541826216 26.769499562750674 27.66093539117235 23.451177504250754 76-77 22.0267223700151 26.534595803594392 27.68378230330913 23.75489952308138 78-79 21.867664443166728 26.514613623544665 27.870252720815913 23.747469212472698 80-81 21.999126596210285 26.619957344360696 27.770837703821243 23.610078355607776 82-83 22.40105402966927 26.327663051264878 27.95126855297448 23.320014366091378 84-85 22.534788620801976 26.51063030674389 27.455245844749655 23.499335227704478 86-87 22.050899465045333 26.754640130491246 27.683806057237398 23.51065434722603 88-89 22.30373042493032 26.22904932208942 27.62386863241502 23.843351620565237 90-91 22.647602947313004 26.019940108714685 27.697868035007367 23.634588908964947 92-93 22.818320062510004 26.169641204946842 27.46491447880764 23.547124253735515 94-95 22.444472680759812 26.05843223102792 27.633931499505664 23.863163588706605 96-97 22.50845074765392 25.908053909944435 27.616239318183812 23.967256024217836 98-99 22.50011557442182 26.076711887125786 27.408753691422827 24.014418847029567 100 22.869079167242724 25.736779674210187 27.189504212852754 24.20463694569434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1052.0 1 835.5 2 1629.0 3 2528.5 4 1846.5 5 1399.5 6 1966.5 7 3637.0 8 5872.5 9 7012.5 10 6746.0 11 6425.0 12 6920.0 13 7739.5 14 8674.0 15 9829.0 16 10996.0 17 11954.0 18 12930.0 19 13941.5 20 15017.5 21 16842.0 22 19610.0 23 23594.0 24 29253.5 25 36247.0 26 45233.0 27 56275.0 28 69792.0 29 88442.0 30 110693.5 31 131319.5 32 156420.0 33 181767.5 34 203966.5 35 227294.0 36 253875.0 37 288640.5 38 308131.5 39 305835.0 40 304037.5 41 304972.0 42 311413.5 43 326036.5 44 343186.0 45 368061.5 46 390336.0 47 409519.5 48 432170.5 49 437787.0 50 429561.0 51 425325.5 52 414095.5 53 396057.0 54 375427.5 55 340285.0 56 303384.5 57 263444.5 58 221969.0 59 191436.0 60 157365.0 61 122495.0 62 95981.5 63 74255.0 64 54130.5 65 37415.0 66 26963.0 67 20405.0 68 15462.5 69 11576.0 70 8918.5 71 6700.5 72 4949.0 73 3808.5 74 2985.0 75 2304.0 76 1701.0 77 1125.5 78 735.5 79 536.5 80 401.0 81 290.5 82 234.0 83 199.5 84 151.5 85 111.0 86 91.0 87 84.5 88 64.5 89 53.5 90 45.5 91 29.5 92 23.5 93 20.5 94 21.0 95 12.0 96 4.0 97 3.5 98 1.5 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2053171061873592 2 0.13710068397416408 3 0.022958194308981095 4 0.021554763375253333 5 0.026471611060313364 6 0.05152043351884754 7 0.058324653838920784 8 0.05108488598769065 9 0.03923799314022317 10-11 0.04159478922548325 12-13 0.039886475019945654 14-15 0.01958996006803446 16-17 0.01818168971729384 18-19 0.017320273489005764 20-21 0.02031103320294976 22-23 0.023263077580790922 24-25 0.020001310514127082 26-27 0.017784857522239784 28-29 0.01781389402431691 30-31 0.01621688641007497 32-33 0.023601836771690726 34-35 0.023655070358832124 36-37 0.022875924219762575 38-39 0.02126439835448207 40-41 0.022208084671988673 42-43 0.027492728050025634 44-45 0.03641177360471622 46-47 0.04160446805950896 48-49 0.038424971082063636 50-51 0.0269023191744574 52-53 0.041769008237946006 54-55 0.04046236564447533 56-57 0.0425433149600027 58-59 0.04560182651212666 60-61 0.023553442601562183 62-63 0.024908479365161405 64-65 0.025368223981382568 66-67 0.030120531488005555 68-69 0.03397754684725048 70-71 0.037578073104814126 72-73 0.04205937325871727 74-75 0.023964793047654802 76-77 0.01803650720690821 78-79 0.017300915820954346 80-81 0.020538485802553914 82-83 0.018805974511952053 84-85 0.011788819843313227 86-87 0.012790579164974081 88-89 0.017876806445484016 90-91 0.018772098592862073 92-93 0.01893663877129912 94-95 0.02260491686704273 96-97 0.023340508252996592 98-99 0.028847764813624853 100 0.02860095454596928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0331823E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.96403322231603 #Duplication Level Percentage of deduplicated Percentage of total 1 74.12513425152221 42.965917443638695 2 14.974461489651564 17.359603665449104 3 4.91458581629009 8.546076465880857 4 2.166921800665101 5.024141089756511 5 1.150574147619407 3.334595905867463 6 0.6724265633345055 2.3385933394013443 7 0.42140142334638225 1.7098288271946673 8 0.28954341386797017 1.3426483248596657 9 0.20199382847473218 1.0537539285970965 >10 1.0073544997032648 10.217962529709654 >50 0.05254547754496786 2.0582310656325147 >100 0.02148537032177835 2.1080633753025175 >500 0.0010252342609728595 0.4096323844795904 >1k 4.7834797377074617E-4 0.571730276894737 >5k 0.0 0.0 >10k+ 6.83354231986157E-5 0.9592213773354561 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31986 0.3095871851463193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23863 0.2309660163554873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16801 0.1626140904659323 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.935766805141745E-5 2 0.0 0.0 0.0 1.935766805141745E-5 1.935766805141745E-5 3 0.0 0.0 0.0 2.9036502077126176E-5 1.935766805141745E-5 4 0.0 0.0 0.0 4.839417012854363E-5 1.935766805141745E-5 5 0.0 9.678834025708725E-6 0.0 4.839417012854363E-5 1.935766805141745E-5 6 9.678834025708725E-6 9.678834025708725E-6 0.0 5.807300415425235E-5 4.839417012854363E-5 7 9.678834025708725E-6 9.678834025708725E-6 0.0 5.807300415425235E-5 4.839417012854363E-5 8 9.678834025708725E-6 9.678834025708725E-6 0.0 6.775183817996108E-5 4.839417012854363E-5 9 9.678834025708725E-6 9.678834025708725E-6 0.0 9.678834025708726E-5 6.775183817996108E-5 10-11 9.678834025708725E-6 9.678834025708725E-6 0.0 1.0162775726994163E-4 6.775183817996108E-5 12-13 9.678834025708725E-6 1.4518251038563088E-5 0.0 1.3550367635992215E-4 9.194892324423289E-5 14-15 9.678834025708725E-6 1.935766805141745E-5 0.0 1.548613444113396E-4 1.8873726350132018E-4 16-17 9.678834025708725E-6 1.935766805141745E-5 0.0 1.645401784370483E-4 2.7584676973269864E-4 18-19 9.678834025708725E-6 1.935766805141745E-5 0.0 2.468102676555725E-4 3.097226888226792E-4 20-21 1.4518251038563088E-5 1.935766805141745E-5 0.0 4.161898631054752E-4 3.145621058355336E-4 22-23 1.935766805141745E-5 1.935766805141745E-5 0.0 0.0010743505768536685 3.4359860791265974E-4 24-25 1.935766805141745E-5 1.935766805141745E-5 0.0 0.0023858325873372006 3.6779569297693156E-4 26-27 1.935766805141745E-5 1.935766805141745E-5 0.0 0.004437745400787451 3.823139440154947E-4 28-29 1.935766805141745E-5 1.935766805141745E-5 0.0 0.012417944054984295 3.968321950540577E-4 30-31 1.935766805141745E-5 1.935766805141745E-5 0.0 0.04266430038532406 3.968321950540577E-4 32-33 1.935766805141745E-5 1.935766805141745E-5 0.0 0.11074037950514637 4.161898631054752E-4 34-35 1.935766805141745E-5 1.935766805141745E-5 0.0 0.20286352176184203 4.258686971311839E-4 36-37 1.935766805141745E-5 3.87153361028349E-5 0.0 0.3513465145502396 4.3554753115689266E-4 38-39 1.935766805141745E-5 5.807300415425235E-5 0.0 0.6036688781834532 4.4522636518260134E-4 40-41 2.4197085064271815E-5 5.807300415425235E-5 0.0 0.9035143168828967 4.500657821954557E-4 42-43 2.9036502077126176E-5 5.807300415425235E-5 0.0 1.1979347691109303 4.6942345024687316E-4 44-45 2.9036502077126176E-5 5.807300415425235E-5 0.0 1.5251858263541682 4.839417012854363E-4 46-47 2.9036502077126176E-5 6.775183817996108E-5 0.0 1.904141214962742 4.887811182982906E-4 48-49 2.9036502077126176E-5 6.775183817996108E-5 0.0 2.3167160335596146 5.226570373882711E-4 50-51 2.9036502077126176E-5 6.775183817996108E-5 0.0 2.767923918170104 5.371752884268343E-4 52-53 2.9036502077126176E-5 6.775183817996108E-5 0.0 3.219780284660316 5.807300415425235E-4 54-55 2.9036502077126176E-5 6.775183817996108E-5 0.0 3.685448347305214 6.291242116710672E-4 56-57 2.9036502077126176E-5 6.775183817996108E-5 0.0 4.188931614488556 6.436424627096302E-4 58-59 2.9036502077126176E-5 6.775183817996108E-5 0.0 4.755607989025751 6.630001307610477E-4 60-61 3.387591908998054E-5 6.775183817996108E-5 0.0 5.343539083083401 6.775183817996108E-4 62-63 3.87153361028349E-5 6.775183817996108E-5 0.0 5.945441574057163 7.452702199795718E-4 64-65 3.87153361028349E-5 6.775183817996108E-5 0.0 6.515084511223237 7.549490540052806E-4 66-67 3.87153361028349E-5 6.775183817996108E-5 0.0 7.118037155688787 7.549490540052806E-4 68-69 3.87153361028349E-5 6.775183817996108E-5 0.0 7.776972176159038 7.549490540052806E-4 70-71 4.355475311568926E-5 7.259125519281544E-5 0.0 8.463966136469818 7.694673050438437E-4 72-73 4.839417012854363E-5 7.74306722056698E-5 0.0 9.138251787704842 8.033432241338242E-4 74-75 4.839417012854363E-5 7.74306722056698E-5 0.0 9.782097505929011 8.13022058159533E-4 76-77 4.839417012854363E-5 7.74306722056698E-5 0.0 10.456044397973137 8.227008921852417E-4 78-79 4.839417012854363E-5 7.74306722056698E-5 0.0 11.17400578774917 8.27540309198096E-4 80-81 4.839417012854363E-5 7.74306722056698E-5 0.0 11.917214416081267 8.66255645300931E-4 82-83 5.807300415425235E-5 7.74306722056698E-5 0.0 12.658787321463016 9.436863175066007E-4 84-85 5.807300415425235E-5 7.74306722056698E-5 0.0 13.372717476867344 9.533651515323095E-4 86-87 6.291242116710671E-5 8.227008921852415E-5 0.0 14.07940302500343 9.8724107062229E-4 88 6.775183817996108E-5 8.710950623137852E-5 0.0 14.617894634857759 0.0010356352407508336 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20765 0.0 37.1389 1 GTATCAA 33365 0.0 35.56717 1 TACCTGG 7885 0.0 33.542713 2 CAACGCA 40160 0.0 29.076187 5 ATCAACG 40350 0.0 28.928043 3 TATCAAC 41815 0.0 28.192865 2 TCAACGC 41935 0.0 27.979988 4 GTACATG 34945 0.0 27.932962 1 AACGCAG 42650 0.0 27.78728 6 ACCTGGG 9625 0.0 27.057951 3 TACATGG 35395 0.0 26.897848 2 ACATGGG 35355 0.0 25.954634 3 ACGCAGA 46750 0.0 25.109106 7 CGCAGAG 47655 0.0 24.572247 8 CATGGGG 26695 0.0 24.266039 4 ATGGGGG 19935 0.0 23.13302 5 GTACTAG 4235 0.0 22.915329 1 GCAGAGT 51475 0.0 22.272434 9 GTACCTG 12580 0.0 21.794888 1 AGAGTAC 42180 0.0 21.313072 10-11 >>END_MODULE