FastQCFastQC Report
Mon 6 Jun 2016
ERR1211371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10331823
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280380.27137514841282123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222200.21506369205124787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171170.16567260201805625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215500.037.262181
GTATCAA339150.033.1725231
TACCTGG70800.030.663812
ATCAACG419250.026.1390443
CAACGCA420350.026.1123265
TATCAAC438350.025.5565742
GTACATG336100.025.4723551
TCAACGC435150.025.2380644
GTACTAG41600.025.0892281
AACGCAG445650.024.9349446
TACATGG339900.024.5809122
ACCTGGG89150.024.1422823
ACATGGG340350.023.334323
ACGCAGA492150.022.3689697
CATGGGG259050.022.0046024
CGCAGAG500550.021.843398
CTAACGC16550.021.295973
ATGGGGG188550.021.2821315
TCTAACG16800.021.2579632
AGAGTAC441350.021.15270610-11
Produced by FastQC (version 0.11.5)