##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211371_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10331823 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.739290345953467 31.0 31.0 34.0 30.0 34.0 2 31.89564406978323 31.0 31.0 34.0 30.0 34.0 3 31.96067663954367 31.0 31.0 34.0 30.0 34.0 4 35.684909816980024 37.0 35.0 37.0 33.0 37.0 5 35.517495702355724 37.0 35.0 37.0 33.0 37.0 6 35.55178742415545 37.0 35.0 37.0 33.0 37.0 7 35.471223326222294 37.0 35.0 37.0 33.0 37.0 8 35.45272087994539 37.0 35.0 37.0 33.0 37.0 9 36.973071547973674 39.0 37.0 39.0 33.0 39.0 10-11 36.93440305742752 39.0 37.0 39.0 33.0 39.0 12-13 36.842087499950395 39.0 37.0 39.0 33.0 39.0 14-15 37.943344654665495 40.0 37.0 41.0 33.0 41.0 16-17 37.92901523767877 40.0 37.0 41.0 33.0 41.0 18-19 37.93253644589149 40.0 37.0 41.0 33.0 41.0 20-21 37.88781916802098 40.0 37.0 41.0 33.0 41.0 22-23 37.773368068732886 39.5 37.0 41.0 32.0 41.0 24-25 37.633226101531164 39.0 36.5 41.0 32.0 41.0 26-27 37.48778294014522 39.0 36.0 41.0 32.0 41.0 28-29 37.405805684050144 39.0 36.0 41.0 32.0 41.0 30-31 37.447756025243564 39.0 36.0 41.0 32.0 41.0 32-33 37.283771605456266 39.0 36.0 41.0 31.5 41.0 34-35 37.1497076072635 39.0 36.0 41.0 31.0 41.0 36-37 36.987935768934484 39.0 36.0 40.0 31.0 41.0 38-39 36.863605725727204 39.0 35.0 40.0 30.0 41.0 40-41 36.79800370176686 39.0 35.0 40.0 30.0 41.0 42-43 36.65673802193476 39.0 35.0 40.0 30.0 41.0 44-45 36.49051348440638 39.0 35.0 40.0 30.0 41.0 46-47 36.25972071917995 38.5 35.0 40.0 29.5 41.0 48-49 36.03060602180274 38.0 35.0 40.0 28.5 41.0 50-51 35.84003868436383 38.0 34.5 40.0 28.0 41.0 52-53 35.619002570988684 38.0 34.0 40.0 27.5 41.0 54-55 35.409579558225104 38.0 34.0 40.0 27.0 41.0 56-57 35.16067662986484 38.0 34.0 40.0 26.5 41.0 58-59 34.881458722240986 37.0 33.5 40.0 26.0 41.0 60-61 34.61084258799246 37.0 33.0 40.0 26.0 41.0 62-63 34.31458427036545 36.5 33.0 39.0 26.0 41.0 64-65 33.952403027035984 36.0 32.5 39.0 25.0 40.0 66-67 33.598561309073915 35.5 32.0 39.0 24.5 40.0 68-69 33.23414667479302 35.0 31.5 38.0 23.5 40.0 70-71 32.86824595233581 35.0 31.0 38.0 23.0 40.0 72-73 32.51331110685888 35.0 31.0 37.0 23.0 39.5 74-75 32.1472813655441 34.5 31.0 36.5 22.0 39.0 76-77 31.24178685600789 34.0 29.5 35.5 21.5 38.0 78-79 31.785487420758177 34.0 31.0 36.0 23.0 38.0 80-81 31.69099204467595 34.0 31.0 35.5 23.0 37.0 82-83 31.41909888506607 34.0 30.5 35.0 22.0 37.0 84-85 31.136385660110513 34.0 30.0 35.0 21.0 36.5 86-87 30.871039215441456 34.0 30.0 35.0 20.0 36.0 88-89 30.625271116239603 34.0 29.5 35.0 20.0 35.5 90-91 30.391583169785235 34.0 29.0 35.0 19.5 35.0 92-93 30.19211377314536 34.0 29.0 35.0 19.0 35.0 94-95 29.996335157890336 33.5 29.0 35.0 19.0 35.0 96-97 29.794950465179284 33.0 29.0 35.0 18.0 35.0 98-99 29.572764748292727 33.0 29.0 35.0 18.0 35.0 100 27.726475085761727 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4470813039917054 1101 2 -0.41558313484810583 1101 3 -0.4715187234190239 1101 4 -0.3118354388319986 1101 5 -0.37090560430873154 1101 6 -0.42457997470663855 1101 7 -0.44300829782257267 1101 8 -0.4992900243537335 1101 9 -0.6761056225741982 1101 10-11 -0.6283984124325599 1101 12-13 -0.6894006312317202 1101 14-15 -0.8316226351303442 1101 16-17 -0.9175841389864772 1101 18-19 -0.8835022809030662 1101 20-21 -0.857305413214938 1101 22-23 -0.9253415376705547 1101 24-25 -1.0126089852704965 1101 26-27 -0.9725479497267884 1101 28-29 -1.0586279914940704 1101 30-31 -1.0479502776510046 1101 32-33 -0.8973580400251535 1101 34-35 -1.1044237145662592 1101 36-37 -1.0700039354915702 1101 38-39 -1.0893165081960703 1101 40-41 -1.091917719980188 1101 42-43 -1.0616097737233332 1101 44-45 -1.098382443744093 1101 46-47 -1.258666206998356 1101 48-49 -1.1337455940689125 1101 50-51 -1.0925058238011403 1101 52-53 -1.1597401321421543 1101 54-55 -1.2334427794235268 1101 56-57 -1.2279606096267344 1101 58-59 -1.2306480587262456 1101 60-61 -1.210460363340239 1101 62-63 -1.3040264370824417 1101 64-65 -1.272565367057254 1101 66-67 -1.3568058943891543 1101 68-69 -1.3602913366810334 1101 70-71 -1.3632919643254908 1101 72-73 -1.411457824032965 1101 74-75 -1.472732882491858 1101 76-77 -1.177096474371794 1101 78-79 -1.0863235094812431 1101 80-81 -1.0039066160664625 1101 82-83 -1.122318052637219 1101 84-85 -1.1428202382564514 1101 86-87 -1.2362083545383662 1101 88-89 -1.2015866465280354 1101 90-91 -1.3986673911590977 1101 92-93 -1.5114521908517595 1101 94-95 -1.571821745811782 1101 96-97 -1.51088146038974 1101 98-99 -1.6933018668807236 1101 100 -1.450008280062768 1102 1 -0.4054280142501945 1102 2 -0.3858179545419631 1102 3 -0.4855131578387315 1102 4 -0.30712385780922347 1102 5 -0.3606211007857567 1102 6 -0.4260894764349814 1102 7 -0.39145148094541327 1102 8 -0.47630895017942976 1102 9 -0.6270892562326509 1102 10-11 -0.6415440794896412 1102 12-13 -0.6502801283045372 1102 14-15 -0.8185859437347887 1102 16-17 -0.8725015064169597 1102 18-19 -0.8605862928255021 1102 20-21 -0.9129213250411112 1102 22-23 -0.8898046574586687 1102 24-25 -0.941246107892141 1102 26-27 -1.0344672401609003 1102 28-29 -1.0535959429924802 1102 30-31 -1.0279167899364623 1102 32-33 -0.9975318270351963 1102 34-35 -1.0061832722380117 1102 36-37 -1.021971278802667 1102 38-39 -1.0671095283289134 1102 40-41 -1.1133949101501415 1102 42-43 -1.0644054711897795 1102 44-45 -1.0350351874966108 1102 46-47 -1.15889053598724 1102 48-49 -1.116298135863957 1102 50-51 -1.1707827807507911 1102 52-53 -1.2398817291845106 1102 54-55 -1.3452418061367695 1102 56-57 -1.3292893211885826 1102 58-59 -1.3675194786694078 1102 60-61 -1.52048101505234 1102 62-63 -1.4000760664295697 1102 64-65 -1.3800391730307737 1102 66-67 -1.438025007137167 1102 68-69 -1.4218910014180715 1102 70-71 -1.4658040573386337 1102 72-73 -1.4262834185471718 1102 74-75 -1.4965120183701615 1102 76-77 -1.2830709735291919 1102 78-79 -1.2084810463623903 1102 80-81 -1.1475539698437167 1102 82-83 -1.163220619206374 1102 84-85 -1.1644587406986737 1102 86-87 -1.221903226661194 1102 88-89 -1.1801765826064035 1102 90-91 -1.2319691011685592 1102 92-93 -1.3493988583664418 1102 94-95 -1.3660647478580152 1102 96-97 -1.4935184843358584 1102 98-99 -1.5131061385957096 1102 100 -1.5798955888701691 1103 1 -0.4787696744223524 1103 2 -0.46682481234656237 1103 3 -0.5064235737360434 1103 4 -0.32938851855399776 1103 5 -0.3382831562030191 1103 6 -0.45085042380249973 1103 7 -0.46131526525878996 1103 8 -0.5653108064209249 1103 9 -0.6895304279494425 1103 10-11 -0.7080364895526756 1103 12-13 -0.7428810234875627 1103 14-15 -0.9984483805104105 1103 16-17 -0.9855631832622507 1103 18-19 -0.9336575982351647 1103 20-21 -0.9622068711619676 1103 22-23 -1.0240484816832662 1103 24-25 -0.9975494875431821 1103 26-27 -1.0959435315449753 1103 28-29 -1.0660613143786861 1103 30-31 -1.0197631157521698 1103 32-33 -1.021572161763011 1103 34-35 -1.0794792770968655 1103 36-37 -1.062802555215086 1103 38-39 -1.0499148077587321 1103 40-41 -1.1895297694503455 1103 42-43 -1.1958501305018245 1103 44-45 -1.2080890168054168 1103 46-47 -1.2928087837005506 1103 48-49 -1.3487069553785247 1103 50-51 -1.4465263999030142 1103 52-53 -1.4396924667305626 1103 54-55 -1.3981189310268363 1103 56-57 -1.4730300742226135 1103 58-59 -1.5669702687840328 1103 60-61 -1.557824072559498 1103 62-63 -1.59652526679794 1103 64-65 -1.5055192245299693 1103 66-67 -1.5906257267590362 1103 68-69 -1.5208625542983611 1103 70-71 -1.4571229466173463 1103 72-73 -1.5748018824501493 1103 74-75 -1.705993716190541 1103 76-77 -1.439194988986479 1103 78-79 -1.190732343279226 1103 80-81 -1.168802517463476 1103 82-83 -1.1812327816877009 1103 84-85 -1.1815591971670827 1103 86-87 -1.2568184216813982 1103 88-89 -1.2800602415719453 1103 90-91 -1.463340686092291 1103 92-93 -1.365145752463718 1103 94-95 -1.4958369518489683 1103 96-97 -1.6140392627278857 1103 98-99 -1.6280366592103093 1103 100 -1.6245486739126491 1104 1 -0.5536204256631834 1104 2 -0.5182124059048938 1104 3 -0.49807398223677524 1104 4 -0.39711224578361737 1104 5 -0.4509333464066785 1104 6 -0.44030421506261774 1104 7 -0.5185514779429425 1104 8 -0.5392856211812429 1104 9 -0.7898862857032327 1104 10-11 -0.7488082487961734 1104 12-13 -0.800314910002264 1104 14-15 -0.931198574666368 1104 16-17 -0.9520287416459112 1104 18-19 -1.0043696296431293 1104 20-21 -0.9392322257790227 1104 22-23 -0.9967978957683528 1104 24-25 -1.066840687385202 1104 26-27 -1.1634046459328715 1104 28-29 -1.205641751466743 1104 30-31 -1.1227539910478797 1104 32-33 -1.0128279308994266 1104 34-35 -1.181825059281529 1104 36-37 -1.1138796550683665 1104 38-39 -1.2566312392008214 1104 40-41 -1.3345954576034202 1104 42-43 -1.2328901808607853 1104 44-45 -1.3015472796848684 1104 46-47 -1.2133859176228938 1104 48-49 -1.2172093061919753 1104 50-51 -1.2521370950523973 1104 52-53 -1.2780203824591112 1104 54-55 -1.2758645196009297 1104 56-57 -1.3310845396962847 1104 58-59 -1.4117193081328878 1104 60-61 -1.2747126139842067 1104 62-63 -1.4168190489484118 1104 64-65 -1.4906562891676671 1104 66-67 -1.559238793805516 1104 68-69 -1.3813555203681602 1104 70-71 -1.3999184656296109 1104 72-73 -1.3789908252477687 1104 74-75 -1.307560985757867 1104 76-77 -1.1842338539399222 1104 78-79 -1.163956882854432 1104 80-81 -1.2774795876249492 1104 82-83 -1.2631000626397189 1104 84-85 -1.1796977813847285 1104 86-87 -1.1414774119533178 1104 88-89 -1.2154385591810133 1104 90-91 -1.1760791749354134 1104 92-93 -1.4151067536421849 1104 94-95 -1.3126463741606855 1104 96-97 -1.5172370598493004 1104 98-99 -1.5145693542139789 1104 100 -1.5526387709648937 1105 1 -0.518602210779072 1105 2 -0.46945612217633226 1105 3 -0.5361646492869205 1105 4 -0.40678735013936773 1105 5 -0.4454173587784709 1105 6 -0.49800657270451865 1105 7 -0.5942170385191972 1105 8 -0.4968674164184179 1105 9 -0.6960182063917202 1105 10-11 -0.7426457352543707 1105 12-13 -0.7223838524627411 1105 14-15 -0.9624476973544773 1105 16-17 -0.9710832919550043 1105 18-19 -0.947678782562285 1105 20-21 -1.0066772966085722 1105 22-23 -0.9640179854722817 1105 24-25 -0.9746202141256717 1105 26-27 -1.0314339463755289 1105 28-29 -1.0975999642853793 1105 30-31 -1.0975925141366645 1105 32-33 -0.9896427958203091 1105 34-35 -1.0358064466445356 1105 36-37 -1.1900227366525868 1105 38-39 -1.1351678108273688 1105 40-41 -1.0862057728907075 1105 42-43 -1.1349224645646245 1105 44-45 -1.1407190697766794 1105 46-47 -1.2241517625340776 1105 48-49 -1.2419141916370862 1105 50-51 -1.3217566055988073 1105 52-53 -1.2661272275413396 1105 54-55 -1.2813428844312327 1105 56-57 -1.2383364427618204 1105 58-59 -1.3193495344215407 1105 60-61 -1.384246856111531 1105 62-63 -1.3054378777962796 1105 64-65 -1.3968934300365348 1105 66-67 -1.3943094584879177 1105 68-69 -1.3880651559491142 1105 70-71 -1.3872111423737543 1105 72-73 -1.4631562070985602 1105 74-75 -1.4195339202422161 1105 76-77 -1.3360906180175007 1105 78-79 -1.2503857007726857 1105 80-81 -1.1849021214139093 1105 82-83 -1.2408575584040058 1105 84-85 -1.0510162829852412 1105 86-87 -1.1320260178374504 1105 88-89 -1.1374784315611812 1105 90-91 -1.12795017064348 1105 92-93 -1.1825167777935945 1105 94-95 -1.1778039307900947 1105 96-97 -1.306505265516627 1105 98-99 -1.3358589918237698 1105 100 -1.4186317689568924 1106 1 -0.46566880070498584 1106 2 -0.44842349438284757 1106 3 -0.4806075485573231 1106 4 -0.3099544340216127 1106 5 -0.3770566596941478 1106 6 -0.4638132143334204 1106 7 -0.4685944074169086 1106 8 -0.5741323661724493 1106 9 -0.6774511559432241 1106 10-11 -0.6754511859592682 1106 12-13 -0.7363774882291381 1106 14-15 -0.8599942701330434 1106 16-17 -0.9643964416227817 1106 18-19 -0.9791511098891732 1106 20-21 -0.8746363000062587 1106 22-23 -0.8679664410683472 1106 24-25 -0.9391944797067353 1106 26-27 -0.9845168046421975 1106 28-29 -1.030418334603084 1106 30-31 -0.9940123204774167 1106 32-33 -1.0029672547494854 1106 34-35 -0.9534180033469042 1106 36-37 -1.007327545949174 1106 38-39 -1.0696756282246724 1106 40-41 -1.0230846598568633 1106 42-43 -0.9582300315322101 1106 44-45 -1.0543155375757252 1106 46-47 -1.0921861479971113 1106 48-49 -1.0982758435498141 1106 50-51 -1.1484204783132412 1106 52-53 -1.193469120643222 1106 54-55 -1.1209901037257453 1106 56-57 -1.2390603202470913 1106 58-59 -1.2608934610492923 1106 60-61 -1.3311239286443595 1106 62-63 -1.265360006600531 1106 64-65 -1.3315911997556213 1106 66-67 -1.287465838663337 1106 68-69 -1.3448338230610446 1106 70-71 -1.2489004924308027 1106 72-73 -1.2571005665584316 1106 74-75 -1.2402810396480994 1106 76-77 -1.1294644959990343 1106 78-79 -1.0014090378200144 1106 80-81 -1.0191492213595161 1106 82-83 -1.0643648022530243 1106 84-85 -0.9797451469838201 1106 86-87 -1.0478125244979388 1106 88-89 -1.0486347988002542 1106 90-91 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2115 80-81 1.004545471274458 2115 82-83 1.0583853727478818 2115 84-85 1.100208593448631 2115 86-87 1.201523249594871 2115 88-89 1.1299376818487978 2115 90-91 1.2697413810317428 2115 92-93 1.3191392238528863 2115 94-95 1.3602009808925075 2115 96-97 1.4004662630815297 2115 98-99 1.4649233847829102 2115 100 1.373503936136082 2116 1 0.25907060968832596 2116 2 0.4224040733426797 2116 3 0.47685313326051926 2116 4 0.33569789117803595 2116 5 0.46292314981987204 2116 6 0.4168627596869001 2116 7 0.5320334618931071 2116 8 0.5642546686274059 2116 9 0.7327633880878466 2116 10-11 0.7435810662321458 2116 12-13 0.6370914661725067 2116 14-15 0.819217111742816 2116 16-17 0.9280522112562508 2116 18-19 0.8105218271518311 2116 20-21 0.9293397316852605 2116 22-23 0.9951459262196778 2116 24-25 1.0432852254721254 2116 26-27 1.0329395347642816 2116 28-29 1.0971822855365332 2116 30-31 0.9260820816066442 2116 32-33 0.9791613749997055 2116 34-35 0.9830734765883093 2116 36-37 1.046506411541145 2116 38-39 0.93988383914769 2116 40-41 1.063091899707004 2116 42-43 1.167269268416348 2116 44-45 1.1611643323216398 2116 46-47 1.1058603401734644 2116 48-49 1.0445662390043324 2116 50-51 1.1597092804909508 2116 52-53 1.200658291744098 2116 54-55 1.2209527495691717 2116 56-57 1.254674402738523 2116 58-59 1.2538433882347206 2116 60-61 1.3273066331798447 2116 62-63 1.2589673848838245 2116 64-65 1.1114910671128442 2116 66-67 1.1971895164679083 2116 68-69 1.148038461683818 2116 70-71 1.047001900105677 2116 72-73 1.2012070160861654 2116 74-75 1.2074156989229152 2116 76-77 1.095776428807838 2116 78-79 1.0514228726149177 2116 80-81 1.0024870111290376 2116 82-83 0.9885759708867674 2116 84-85 0.9562367276870063 2116 86-87 0.923702513093442 2116 88-89 1.0217790038651877 2116 90-91 0.8371662277562208 2116 92-93 0.9778539352452214 2116 94-95 1.0401105646241469 2116 96-97 1.1618837343491037 2116 98-99 1.3063875675184349 2116 100 0.8171775368513536 2201 1 0.7734951172949813 2201 2 0.7248938382516421 2201 3 0.726232651037158 2201 4 0.5149773711503869 2201 5 0.6139413018322841 2201 6 0.634364460712959 2201 7 0.6713791363735879 2201 8 0.6754947964720088 2201 9 0.8302088804133092 2201 10-11 0.7801213122193147 2201 12-13 0.8178129475831355 2201 14-15 1.0030928537090205 2201 16-17 1.0026340416548791 2201 18-19 0.9806454801166922 2201 20-21 0.9724972514279031 2201 22-23 0.9460429635909477 2201 24-25 0.9300790791703051 2201 26-27 0.9754196509270585 2201 28-29 0.9959183110538135 2201 30-31 1.1285356072043342 2201 32-33 1.0178258934323878 2201 34-35 1.1454997258130604 2201 36-37 1.1746505658207056 2201 38-39 1.2372113210515465 2201 40-41 1.220251325439051 2201 42-43 0.2904690436605577 2201 44-45 1.0457225289190717 2201 46-47 1.2382665537929611 2201 48-49 1.3090075127350929 2201 50-51 1.3046590589068572 2201 52-53 1.330016012933406 2201 54-55 1.3667854082233717 2201 56-57 1.3497820205975941 2201 58-59 1.3302157926954266 2201 60-61 1.320100155881839 2201 62-63 1.388419352487432 2201 64-65 1.4020986336128374 2201 66-67 1.4109769732620165 2201 68-69 1.3927549937874701 2201 70-71 1.3842557799418955 2201 72-73 1.4188250436181917 2201 74-75 1.3792541775679439 2201 76-77 1.310592941681044 2201 78-79 1.1867306133796625 2201 80-81 1.1108208786413094 2201 82-83 1.0862078589711608 2201 84-85 0.9862272686856279 2201 86-87 1.0110621506218713 2201 88-89 1.0065962855943873 2201 90-91 0.37438514530269984 2201 92-93 0.7485203898261545 2201 94-95 1.0468537251583818 2201 96-97 1.0308541617579152 2201 98-99 1.086734759901752 2201 100 1.207118966912919 2202 1 0.778772056207341 2202 2 0.7493075712526114 2202 3 0.7397476326003556 2202 4 0.5423759196080411 2202 5 0.5404783096417702 2202 6 0.5933656159579215 2202 7 0.6671526865897377 2202 8 0.6887157052781063 2202 9 0.8922683504632474 2202 10-11 0.8631407688354997 2202 12-13 0.8901269821178914 2202 14-15 1.0660142914692585 2202 16-17 1.0674037722607395 2202 18-19 1.044029568529389 2202 20-21 1.0277340409165276 2202 22-23 1.0456267999085753 2202 24-25 1.035216370158274 2202 26-27 1.0926522907672975 2202 28-29 1.0705354875266124 2202 30-31 1.2501838672136145 2202 32-33 0.8249187563922149 2202 34-35 1.2041011160035993 2202 36-37 1.2850946900708848 2202 38-39 1.3197565293473374 2202 40-41 1.350154591956425 2202 42-43 1.4010272814268774 2202 44-45 1.3747984085945149 2202 46-47 1.351576027750653 2202 48-49 1.3440053319269225 2202 50-51 1.359971400712432 2202 52-53 1.3548787961928426 2202 54-55 1.412621569098853 2202 56-57 1.4100774030119254 2202 58-59 1.4173105805052728 2202 60-61 1.3750497949278042 2202 62-63 1.4164817802078602 2202 64-65 1.4838295209351955 2202 66-67 1.4700266670500852 2202 68-69 1.4559953743941847 2202 70-71 1.4340190842394733 2202 72-73 1.4560826914358458 2202 74-75 1.4014228058175462 2202 76-77 1.36610451634904 2202 78-79 1.1787505106793041 2202 80-81 1.1484411175683427 2202 82-83 1.1562513045888956 2202 84-85 1.1248033717731865 2202 86-87 1.1991460394709392 2202 88-89 1.2066051380622262 2202 90-91 1.2134912422762838 2202 92-93 1.208787233045335 2202 94-95 1.1446790633301909 2202 96-97 1.0365889364064316 2202 98-99 0.9846399726501573 2202 100 1.2336497139355984 2203 1 0.776255025373711 2203 2 0.7253841531907419 2203 3 0.7060676366870737 2203 4 0.46447896537034694 2203 5 0.6182253037091172 2203 6 0.6244738303668811 2203 7 0.6345698707835723 2203 8 0.6342687466361951 2203 9 0.8258580369369071 2203 10-11 0.8550982953152015 2203 12-13 0.8153842331858812 2203 14-15 1.014249178179952 2203 16-17 0.9573950913210538 2203 18-19 0.9792755252923584 2203 20-21 1.0226286867092682 2203 22-23 0.9761403028656588 2203 24-25 0.9597302996205812 2203 26-27 1.0086324490242333 2203 28-29 1.0579933963859958 2203 30-31 1.15570700153868 2203 32-33 1.2056858374556256 2203 34-35 1.250900647631191 2203 36-37 1.223204765252639 2203 38-39 1.2260248811346486 2203 40-41 1.2578848991526854 2203 42-43 1.3372437500786134 2203 44-45 1.3956829769304733 2203 46-47 1.40134203321756 2203 48-49 1.4067544131031582 2203 50-51 1.3509468943288923 2203 52-53 1.3713555188660322 2203 54-55 1.3588793899238212 2203 56-57 1.4508970072187424 2203 58-59 1.4555214235881024 2203 60-61 1.4356473705814352 2203 62-63 1.4382734221554756 2203 64-65 1.4755447736137128 2203 66-67 1.5237912839999765 2203 68-69 1.4444961710289448 2203 70-71 1.4822140912949706 2203 72-73 1.51026920938736 2203 74-75 1.5457504071640003 2203 76-77 1.4386229463664648 2203 78-79 1.2603566145207488 2203 80-81 1.2764908945114435 2203 82-83 1.1957296215316155 2203 84-85 1.2300105772281178 2203 86-87 1.2182210231394883 2203 88-89 1.2746071585869778 2203 90-91 1.3607636505299538 2203 92-93 1.442490491743584 2203 94-95 1.4517183245611385 2203 96-97 1.4072980400929538 2203 98-99 1.3558354364612555 2203 100 1.5635612553519813 2204 1 0.7795592143629371 2204 2 0.7766676179159511 2204 3 0.7481562531581432 2204 4 0.5048183895464433 2204 5 0.6367067918087983 2204 6 0.6497579400037168 2204 7 0.6704143913113114 2204 8 0.693746333956355 2204 9 0.8927110902734157 2204 10-11 0.8727314271386533 2204 12-13 0.9080705493917307 2204 14-15 1.0950651658279469 2204 16-17 1.077907876366048 2204 18-19 1.0450704472565562 2204 20-21 1.0453382539074596 2204 22-23 1.0675173865186807 2204 24-25 1.0196716920802515 2204 26-27 1.065060949602227 2204 28-29 1.1350941505445604 2204 30-31 1.2129987596252576 2204 32-33 1.2812461672975246 2204 34-35 1.2794281492291901 2204 36-37 1.3565768959141522 2204 38-39 1.3773928327643858 2204 40-41 1.3097812361502434 2204 42-43 1.3793497821509106 2204 44-45 1.4138939682685674 2204 46-47 1.4120628498801793 2204 48-49 1.3704630371595385 2204 50-51 1.4189371309318304 2204 52-53 1.4414029408219733 2204 54-55 1.4497726357001213 2204 56-57 1.4818075926856267 2204 58-59 1.4678672693115544 2204 60-61 1.413897867518699 2204 62-63 1.4522035669046076 2204 64-65 1.4980706617131148 2204 66-67 1.4911162590231086 2204 68-69 1.5261985387874972 2204 70-71 1.5459473952177305 2204 72-73 1.5467746526670965 2204 74-75 1.5180924729757663 2204 76-77 1.4505943532377898 2204 78-79 1.2767472130776945 2204 80-81 1.2231284886339644 2204 82-83 1.1798298798456734 2204 84-85 1.2213080040312398 2204 86-87 1.245621121475292 2204 88-89 1.2575095051179552 2204 90-91 1.3521344838867506 2204 92-93 1.3188058612062505 2204 94-95 1.3524518639302165 2204 96-97 1.3003690163686699 2204 98-99 1.3363520066248 2204 100 1.518463898509971 2205 1 0.773969016031959 2205 2 0.5824241872488791 2205 3 0.6863100200952914 2205 4 0.5163998924740056 2205 5 0.6331644119779583 2205 6 0.668709641005222 2205 7 0.711427766080547 2205 8 0.7063006491342563 2205 9 0.8919258755271287 2205 10-11 0.9143896812837298 2205 12-13 0.9058123314809592 2205 14-15 1.12006807825329 2205 16-17 1.070607845977328 2205 18-19 1.125961972782882 2205 20-21 1.1167525815476438 2205 22-23 1.1017547601702091 2205 24-25 1.122118585403392 2205 26-27 1.1591913657287307 2205 28-29 1.2034156068484307 2205 30-31 1.2593692350533061 2205 32-33 1.3266197848282957 2205 34-35 1.333510436749627 2205 36-37 1.3211256316728068 2205 38-39 1.3706771530540607 2205 40-41 1.3422363298681503 2205 42-43 1.4315322562738686 2205 44-45 1.4101450238931932 2205 46-47 1.4254276691916488 2205 48-49 1.4439957655652051 2205 50-51 1.4403485357821637 2205 52-53 1.4580992223285065 2205 54-55 1.4802793065015365 2205 56-57 1.4893030976006827 2205 58-59 1.481413052845177 2205 60-61 1.5067683746621157 2205 62-63 1.5038383806897286 2205 64-65 1.535730083315812 2205 66-67 1.5506918367715983 2205 68-69 1.5621445007564034 2205 70-71 1.6153659341627602 2205 72-73 1.6657247368061334 2205 74-75 1.592118344433537 2205 76-77 1.516442839129006 2205 78-79 1.3780402262833142 2205 80-81 1.2895543809865764 2205 82-83 1.2867416974105232 2205 84-85 1.3504399686144914 2205 86-87 1.4356013211287895 2205 88-89 1.4152216903324515 2205 90-91 1.4178161012798824 2205 92-93 1.4816827819041194 2205 94-95 1.5566808713549314 2205 96-97 1.486366655749471 2205 98-99 1.4944486112550806 2205 100 1.6868932907479532 2206 1 0.8265771962685342 2206 2 0.8104605956566964 2206 3 0.7770374403971019 2206 4 0.4674155759062941 2206 5 0.6491818964867662 2206 6 0.6470309604633968 2206 7 0.6968932637824921 2206 8 0.7085456838540694 2206 9 0.8981620947568985 2206 10-11 0.918403646857044 2206 12-13 0.9299946830640664 2206 14-15 1.1634819881223422 2206 16-17 1.1575120535300627 2206 18-19 1.1684469813459017 2206 20-21 1.160673624549176 2206 22-23 1.1558867170728746 2206 24-25 1.1787610102342256 2206 26-27 1.265702017639093 2206 28-29 1.3062113496409893 2206 30-31 1.363877024994899 2206 32-33 1.3748447939098583 2206 34-35 1.423386236677537 2206 36-37 1.435488106405792 2206 38-39 1.4892367218506237 2206 40-41 1.4667822223038627 2206 42-43 1.5027808626493737 2206 44-45 1.5382963725833676 2206 46-47 1.5389447294039797 2206 48-49 1.5745940526341258 2206 50-51 1.5142119800598621 2206 52-53 1.5721358536479428 2206 54-55 1.5330439793122679 2206 56-57 1.6083040109183955 2206 58-59 1.5951159734366058 2206 60-61 1.5538690670146877 2206 62-63 1.6487928026707834 2206 64-65 1.7011307493555634 2206 66-67 1.7016211360402806 2206 68-69 1.730891211818367 2206 70-71 1.7219864986293132 2206 72-73 1.677850980593746 2206 74-75 1.6782391959979606 2206 76-77 1.5937191874678405 2206 78-79 1.4818783028717668 2206 80-81 1.439551764677656 2206 82-83 1.4299014720076322 2206 84-85 1.4062056147394237 2206 86-87 1.4868098877332478 2206 88-89 1.4902957947871123 2206 90-91 1.5599335749163004 2206 92-93 1.6024968485258029 2206 94-95 1.6139736564706766 2206 96-97 1.544396525486441 2206 98-99 1.6507098942514702 2206 100 1.8420766071869181 2207 1 0.8101425609097923 2207 2 0.7733752275853654 2207 3 0.7423000307857706 2207 4 0.5259295582484 2207 5 0.6200878113855666 2207 6 0.6565046565842678 2207 7 0.703228037111451 2207 8 0.7144123322284273 2207 9 0.9049941444461922 2207 10-11 0.8844772251622359 2207 12-13 0.9071156488519705 2207 14-15 1.135691989715582 2207 16-17 1.1398373244436542 2207 18-19 1.1187007622740595 2207 20-21 1.1112702487954422 2207 22-23 1.1187152829465532 2207 24-25 1.1777778116325024 2207 26-27 1.190506235043415 2207 28-29 1.229546649188194 2207 30-31 1.301070891036261 2207 32-33 1.3125772406842486 2207 34-35 1.3454891101287174 2207 36-37 1.388577948497634 2207 38-39 1.3922837299721849 2207 40-41 1.415034829946869 2207 42-43 1.489286636343266 2207 44-45 1.5360376624746692 2207 46-47 1.5286479709748164 2207 48-49 1.5362938527675851 2207 50-51 1.572246358917667 2207 52-53 1.5685292315171893 2207 54-55 1.5879643175303855 2207 56-57 1.5967771116224654 2207 58-59 1.5246806311104422 2207 60-61 1.5252628554394434 2207 62-63 1.6428999036258176 2207 64-65 1.6582753542357551 2207 66-67 1.651825237787115 2207 68-69 1.670965186980787 2207 70-71 1.6549811806323902 2207 72-73 1.6969158558816488 2207 74-75 1.6781561616367782 2207 76-77 1.561674956534958 2207 78-79 1.4453285624148826 2207 80-81 1.4319828104061578 2207 82-83 1.4376550191090338 2207 84-85 1.4510813489495327 2207 86-87 1.4720604419700898 2207 88-89 1.4957600267325653 2207 90-91 1.586365053646631 2207 92-93 1.5908055305870832 2207 94-95 1.6512717865874116 2207 96-97 1.6211526482049052 2207 98-99 1.6772262735043952 2207 100 1.8623783742623665 2208 1 0.8465493192221238 2208 2 0.7827509175345035 2208 3 0.7345413799625575 2208 4 0.5346578350269411 2208 5 0.6335505397167154 2208 6 0.647681374088954 2208 7 0.7161612131376245 2208 8 0.7096230581945946 2208 9 0.8729911102384591 2208 10-11 0.8834332826281539 2208 12-13 0.9334815278176762 2208 14-15 1.1270000869811199 2208 16-17 1.1337765273576466 2208 18-19 1.1057648227168144 2208 20-21 1.0859364366492343 2208 22-23 1.1608471066649457 2208 24-25 1.1729902554693368 2208 26-27 1.1855861040789364 2208 28-29 1.2264311787022564 2208 30-31 1.2733668655125854 2208 32-33 1.3393633257526076 2208 34-35 1.3888919340690862 2208 36-37 1.3421710915944658 2208 38-39 1.3630630487214432 2208 40-41 1.3856596897163342 2208 42-43 1.4600780548772008 2208 44-45 1.4920809372798942 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52-53 1.4785680941219468 2310 54-55 1.5250484247165375 2310 56-57 1.5132910391963819 2310 58-59 1.4926264613874238 2310 60-61 1.5069051619746716 2310 62-63 1.5887061099789364 2310 64-65 1.6201495524584004 2310 66-67 1.5997776756318487 2310 68-69 1.5472892806931284 2310 70-71 1.5542394838307345 2310 72-73 1.66826093604854 2310 74-75 1.6938949508891525 2310 76-77 1.5681992144266417 2310 78-79 1.386332426466364 2310 80-81 1.3729706055970077 2310 82-83 1.3870862232477883 2310 84-85 1.4329211065088394 2310 86-87 1.5055081346410866 2310 88-89 1.5516829800208818 2310 90-91 1.5986977539452383 2310 92-93 1.6520466104751321 2310 94-95 1.665003228947679 2310 96-97 1.7093693670731582 2310 98-99 1.728153947118507 2310 100 1.7753039096990122 2311 1 0.5084612154801214 2311 2 0.5848677867414445 2311 3 0.6345401028833635 2311 4 0.4406008431494328 2311 5 0.08608489693052945 2311 6 0.29466691302509673 2311 7 0.507439853224362 2311 8 0.3445500843890841 2311 9 0.534934247601619 2311 10-11 0.6867811228640832 2311 12-13 0.749182397925928 2311 14-15 0.9139285851075911 2311 16-17 1.0413710056021088 2311 18-19 1.0694940380839384 2311 20-21 1.1110803580965083 2311 22-23 1.0893463601596665 2311 24-25 1.053981992406328 2311 26-27 1.0696345039763102 2311 28-29 1.0348707137070932 2311 30-31 1.1100134218421474 2311 32-33 1.2525998664440223 2311 34-35 1.2310852484764396 2311 36-37 1.3269773526597035 2311 38-39 1.3530569497183862 2311 40-41 1.3067467478956445 2311 42-43 1.4361278225051421 2311 44-45 1.412575032714237 2311 46-47 1.4456593695846394 2311 48-49 1.3863591824989072 2311 50-51 1.4297465389841975 2311 52-53 1.481499627568887 2311 54-55 1.4357293515381215 2311 56-57 1.5043184709396442 2311 58-59 1.52962051893725 2311 60-61 1.5610327597725302 2311 62-63 1.5542705893744824 2311 64-65 1.6342030750825174 2311 66-67 1.7110810312570166 2311 68-69 1.6968455789210992 2311 70-71 1.672591111202287 2311 72-73 1.6984768847460074 2311 74-75 1.5925671093815055 2311 76-77 1.4088220654072998 2311 78-79 1.3387703592448297 2311 80-81 1.3670895809557244 2311 82-83 1.3840810128389514 2311 84-85 1.3839984314486848 2311 86-87 1.4987028155103168 2311 88-89 1.5213922208697888 2311 90-91 1.5598437919305042 2311 92-93 1.6038940296906574 2311 94-95 1.6330023935059863 2311 96-97 1.6912379590072533 2311 98-99 1.6836328892129373 2311 100 1.7438974837932406 2312 1 0.30792571022202964 2312 2 0.36916163237802735 2312 3 0.5000325139082449 2312 4 0.4108639321997245 2312 5 0.49173146635524745 2312 6 0.602346343385868 2312 7 0.6352652924039504 2312 8 0.450658398646695 2312 9 0.6137359420064499 2312 10-11 0.4873864948248041 2312 12-13 0.649439285208814 2312 14-15 1.0405503922979449 2312 16-17 1.0777456056606738 2312 18-19 1.1232166292260857 2312 20-21 1.141845229580369 2312 22-23 0.7509672034108377 2312 24-25 0.7681126684021464 2312 26-27 1.0800333144945427 2312 28-29 1.2071439451269441 2312 30-31 1.2058749540840026 2312 32-33 1.0804391009436287 2312 34-35 1.2073454638794843 2312 36-37 1.2983303602394543 2312 38-39 1.3278246783426013 2312 40-41 1.390590292421706 2312 42-43 1.298024990542885 2312 44-45 1.2523031710111354 2312 46-47 1.3902079383232007 2312 48-49 1.464311736832336 2312 50-51 1.5239491282836752 2312 52-53 1.4951969378935175 2312 54-55 1.516187484281474 2312 56-57 1.4818049454756164 2312 58-59 1.5320248942091084 2312 60-61 1.5765266456249876 2312 62-63 1.5828235496327707 2312 64-65 1.652282645401442 2312 66-67 1.6720377583176287 2312 68-69 1.6413082991083172 2312 70-71 1.555247210654251 2312 72-73 1.6257159552102465 2312 74-75 1.6875986209804736 2312 76-77 1.526573806887896 2312 78-79 1.3992284238020112 2312 80-81 1.2527541413722254 2312 82-83 1.2477093414844695 2312 84-85 1.1848110601731072 2312 86-87 1.297797895466477 2312 88-89 0.9640322116238984 2312 90-91 1.3494421536541914 2312 92-93 1.5823555696856815 2312 94-95 1.655685492397179 2312 96-97 1.6929131866363818 2312 98-99 1.6709221959994096 2312 100 1.7521243869109355 2313 1 -0.16334013429016636 2313 2 0.1880445689864345 2313 3 0.4014651778898255 2313 4 0.2645964900734441 2313 5 0.42065238062293275 2313 6 0.493013546326857 2313 7 0.3061053659711561 2313 8 0.24217575721494455 2313 9 0.44337437549722125 2313 10-11 0.5909411005411727 2313 12-13 0.7505602226802921 2313 14-15 0.8374939016117864 2313 16-17 0.9795104229926395 2313 18-19 0.8478765017058336 2313 20-21 1.0324012787664856 2313 22-23 1.0786751491692712 2313 24-25 0.9340202972561187 2313 26-27 1.1812614598636841 2313 28-29 1.0863252600644913 2313 30-31 1.0926666239061902 2313 32-33 1.2502740929670608 2313 34-35 1.2540418121538721 2313 36-37 1.1552789591720298 2313 38-39 0.9248322255384878 2313 40-41 1.1026483701575245 2313 42-43 1.2095131613937369 2313 44-45 1.366719173593303 2313 46-47 1.3963491738022427 2313 48-49 1.3551062181520308 2313 50-51 1.3352394458299628 2313 52-53 1.299323818641021 2313 54-55 1.3267092442528252 2313 56-57 1.4301688705017241 2313 58-59 1.377090751102898 2313 60-61 1.4207899423328882 2313 62-63 1.4692467604272679 2313 64-65 1.5571807387265935 2313 66-67 1.5762152382863448 2313 68-69 1.5357878035172448 2313 70-71 1.464977393128244 2313 72-73 1.5426005296558358 2313 74-75 1.1851603244883506 2313 76-77 1.289604234192069 2313 78-79 1.1615552153611297 2313 80-81 1.2352898784859612 2313 82-83 1.227635420067319 2313 84-85 1.2277563514853433 2313 86-87 1.3176242483056733 2313 88-89 1.3347424661732 2313 90-91 1.442624415016173 2313 92-93 1.1741129848548972 2313 94-95 1.201551242469126 2313 96-97 1.523981998102112 2313 98-99 1.57140705497131 2313 100 1.5414624507573436 2314 1 -0.24289381450389058 2314 2 -0.03623187757883173 2314 3 0.23652461056446583 2314 4 0.0031585824624400516 2314 5 0.3662660268099458 2314 6 0.38470582495619254 2314 7 0.3611080035132517 2314 8 0.37955130708119356 2314 9 0.4638181555901326 2314 10-11 0.6377924219082729 2314 12-13 0.7052692943374836 2314 14-15 0.8197961164615535 2314 16-17 1.0170082347939413 2314 18-19 1.0602929991227512 2314 20-21 0.6815411281119736 2314 22-23 0.911784820502092 2314 24-25 1.1444927188441412 2314 26-27 1.1271988137778024 2314 28-29 1.1292455297145096 2314 30-31 1.1162782171856875 2314 32-33 1.197315212524508 2314 34-35 1.1953123547728808 2314 36-37 1.0937013801295876 2314 38-39 1.1233066933937366 2314 40-41 1.1895861272133175 2314 42-43 1.0686553857806302 2314 44-45 1.1229098116872862 2314 46-47 1.1987879514691997 2314 48-49 1.1068526423293719 2314 50-51 1.232852228311792 2314 52-53 1.323358030940895 2314 54-55 1.3653096112930356 2314 56-57 1.293996207005918 2314 58-59 1.2387460368777496 2314 60-61 1.3347381304815826 2314 62-63 1.0862557928100642 2314 64-65 1.2399257915865576 2314 66-67 1.3711535585713506 2314 68-69 1.3742668442989014 2314 70-71 1.457622886497198 2314 72-73 1.5193726746193121 2314 74-75 1.494822869708429 2314 76-77 1.201708092460592 2314 78-79 1.1826349320855876 2314 80-81 1.1709851196679075 2314 82-83 1.1458917763706644 2314 84-85 1.0730513674840303 2314 86-87 1.2197853518047204 2314 88-89 1.2815582872715545 2314 90-91 1.38077934583454 2314 92-93 1.4856433719841817 2314 94-95 1.5701589034125831 2314 96-97 1.6393004096476638 2314 98-99 1.6437736216347538 2314 100 1.6265114902774052 2315 1 0.08155656092973729 2315 2 0.26276091858335704 2315 3 0.2613771822785722 2315 4 0.09301472554719226 2315 5 0.42867247824445087 2315 6 0.536901274450031 2315 7 0.5980538798820376 2315 8 0.5530856212376563 2315 9 0.7561836953608463 2315 10-11 0.765018985613743 2315 12-13 0.8399102067072306 2315 14-15 0.9609743508487867 2315 16-17 0.9704188264004046 2315 18-19 1.0617352700307237 2315 20-21 1.0260631920308967 2315 22-23 1.1370585241203983 2315 24-25 1.0678808775337316 2315 26-27 1.2160781965429521 2315 28-29 1.2357884043652376 2315 30-31 1.2059657136418807 2315 32-33 1.201568695494096 2315 34-35 1.2067769652364646 2315 36-37 1.2503103314793478 2315 38-39 1.2476145403422336 2315 40-41 1.2636701399530992 2315 42-43 1.3085953520733398 2315 44-45 1.205420425217902 2315 46-47 1.2263516019714373 2315 48-49 1.3200179636323384 2315 50-51 1.2448457072644246 2315 52-53 1.2876516929124548 2315 54-55 1.1895731643211533 2315 56-57 1.3290711668586184 2315 58-59 1.383852612471216 2315 60-61 1.4704102215197707 2315 62-63 1.4531213720231833 2315 64-65 1.4579028804082057 2315 66-67 1.4441010130964784 2315 68-69 1.354504174294462 2315 70-71 0.9850788718583416 2315 72-73 1.3012174123253217 2315 74-75 1.467825553287426 2315 76-77 1.3444789293526185 2315 78-79 1.1887154984025976 2315 80-81 1.1862320929666375 2315 82-83 1.1546349335645125 2315 84-85 1.0520993374825416 2315 86-87 1.1214063314077727 2315 88-89 1.2186348403322107 2315 90-91 1.343588367115629 2315 92-93 1.3783383649675258 2315 94-95 1.5143230498350206 2315 96-97 1.5790265544535451 2315 98-99 1.4718661121696215 2315 100 1.4275684873887577 2316 1 0.5656137528060903 2316 2 0.5881871702387222 2316 3 0.5945979433166002 2316 4 0.4275218083470236 2316 5 0.5165616436445859 2316 6 0.5526135479979999 2316 7 0.6131223710964235 2316 8 0.656361650500088 2316 9 0.6956487995801055 2316 10-11 0.650502531429261 2316 12-13 0.8059781163028319 2316 14-15 0.9885595114652403 2316 16-17 0.9800955118632544 2316 18-19 0.9896878231173716 2316 20-21 1.0718826670201125 2316 22-23 1.0903878082223812 2316 24-25 1.1290101257888594 2316 26-27 1.1718751443597242 2316 28-29 1.1476925846227743 2316 30-31 1.080388894358343 2316 32-33 1.0749863474871901 2316 34-35 0.9678661182172945 2316 36-37 0.9452076724307474 2316 38-39 0.8854921808413891 2316 40-41 0.9715456246206955 2316 42-43 1.1712135546589053 2316 44-45 1.0833924054029183 2316 46-47 1.126277781312865 2316 48-49 0.894478882977026 2316 50-51 1.0058846048288004 2316 52-53 1.1615108261119573 2316 54-55 1.2282001926802693 2316 56-57 1.303163188233512 2316 58-59 1.2302029536688508 2316 60-61 1.2726482395799437 2316 62-63 1.301454333114485 2316 64-65 1.328828379185829 2316 66-67 1.3062928260276152 2316 68-69 1.2172581216431126 2316 70-71 1.1216035276098069 2316 72-73 1.1439151074828189 2316 74-75 1.2290893222875638 2316 76-77 1.087739220143007 2316 78-79 0.9997703246522853 2316 80-81 1.0309920971393858 2316 82-83 0.9438525186624993 2316 84-85 0.9973207233607795 2316 86-87 0.9372435589849992 2316 88-89 0.8860771875877624 2316 90-91 0.9970427039481748 2316 92-93 1.006982933645915 2316 94-95 0.950563749841276 2316 96-97 1.131051414242549 2316 98-99 1.1672786769997785 2316 100 0.8368209198977468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 17.0 12 63.0 13 367.0 14 1429.0 15 3987.0 16 9099.0 17 17926.0 18 28512.0 19 41174.0 20 55779.0 21 73873.0 22 88162.0 23 95083.0 24 102492.0 25 118107.0 26 142629.0 27 176283.0 28 214902.0 29 261848.0 30 320907.0 31 393394.0 32 489231.0 33 625523.0 34 829520.0 35 1091792.0 36 1527024.0 37 2000956.0 38 1460653.0 39 161081.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.76789913311957 20.95827564766197 14.067766788140254 24.206058431078205 2 15.231484317917566 21.521313324860483 39.85608348110493 23.391118876117023 3 17.480158148276445 27.332320733717562 31.27190622603581 23.915614891970176 4 12.137194712018516 17.812732536466026 39.13740157781392 30.91267117370154 5 12.54536748570846 38.333283485601214 35.18181306945589 13.939535959234428 6 29.51083269622408 38.609149614738854 18.41504640565368 13.464971283383386 7 26.02449639017682 33.088100359588566 23.55823879034531 17.329164459889306 8 25.749313236404053 35.60239393081201 20.57632972550556 18.071963107278368 9 26.581004895543696 15.211513834055983 20.6887173102511 37.518763960149215 10-11 24.606418760247223 26.27081548485222 29.05351134944414 20.069254405456416 12-13 25.23529795503085 24.13976701432491 28.831212662583088 21.793722368061154 14-15 22.584458623188297 25.483280074724497 26.56572252351099 25.366538778576224 16-17 21.86937250954322 28.015582354870965 27.347882600873252 22.76716253471256 18-19 21.58241580406478 27.559545880722112 29.244955125537864 21.61308318967524 20-21 22.61617663448793 26.75102836416599 28.726841631942634 21.90595336940345 22-23 22.421178721315687 26.684017912424558 28.5971217276951 22.297681638564658 24-25 22.09454750505272 26.718654323106843 28.993018310486985 22.193779861353452 26-27 22.122285020119637 26.753360963504836 28.772714472467413 22.351639543908107 28-29 21.860839600146715 27.360445934588267 28.73841331365352 22.040301151611498 30-31 22.560912047640375 26.82307367170741 28.60867785981085 22.007336420841366 32-33 22.604817778665772 26.97536804949739 28.34779743367249 22.072016738164354 34-35 22.616323096014103 27.026683480707902 28.69970865859229 21.65728476468571 36-37 22.391426654156497 26.894613562278096 28.67459224877412 22.03936753479129 38-39 22.352602007195344 26.896086512173547 28.80265489818376 21.948656582447356 40-41 22.300343976615125 26.52573262353817 29.051491186391694 22.122432213455014 42-43 22.169962121267115 26.712554764943274 28.60186969077074 22.51561342301887 44-45 21.923071673749078 27.125969401005307 28.637319762001578 22.313639163244037 46-47 22.024910164616408 26.769976309130133 28.367759663027048 22.837353863226415 48-49 22.211847091501973 26.72603882811205 28.410734959655237 22.65137912073074 50-51 22.04433869466762 26.985894530246483 28.12721483907576 22.842551936010135 52-53 21.786092123715356 27.21609956486642 28.040037400326863 22.95777091109136 54-55 21.702143064703133 26.999510013932948 28.235689106066786 23.06265781529713 56-57 22.14870310397672 26.850479303547104 27.956014937928497 23.044802654547674 58-59 21.80336573380345 26.97564793398181 28.27085042292489 22.95013590928985 60-61 21.989056654122546 27.31217659803479 27.837377445352114 22.86138930249055 62-63 21.935130809448857 27.0580935690831 27.70495906500218 23.30181655646587 64-65 21.470033301360296 26.928574509040775 27.824426163704686 23.77696602589424 66-67 21.63000346932446 26.73486798896052 27.708341261073784 23.926787280641236 68-69 21.379253612365243 26.8660175048263 27.823046394452 23.931682488356454 70-71 21.582769479845915 26.50731345267153 27.869746152760538 24.04017091472202 72-73 21.623742173805613 26.738319479913898 27.6685357357804 23.969402610500094 74-75 21.59932858203859 26.88727749998076 27.43248026986061 24.080913648120042 76-77 21.477538820372622 26.656184708617154 27.528466207907947 24.337810263102277 78-79 21.298744807783883 26.65000827385987 27.67624277416641 24.375004144189834 80-81 21.28471107120782 26.77324116602524 27.533105567109057 24.408942195657886 82-83 21.560093394360276 26.429471259838806 27.775339464587084 24.235095881213837 84-85 21.690918644451994 26.539017730387094 27.269987880345365 24.500075744815547 86-87 21.16236719044575 26.72311336987438 27.45365669634376 24.660862743336114 88-89 21.2826351656234 26.348828503830052 27.42056729206148 24.94796903848507 90-91 21.466992089812607 26.052377713142384 27.559091138812548 24.921539058232465 92-93 21.489115906921917 26.213928719340903 27.330534030858093 24.966421342879087 94-95 20.92242001714615 26.04312110239457 27.636907602933757 25.397551277525526 96-97 20.937626222711817 25.89389031017067 27.68795756564839 25.48052590146912 98-99 20.876853986732506 26.100421505120686 27.505538610878784 25.51718589726803 100 20.94897466855529 25.867518147270722 27.505012619642656 25.678494564531327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1114.0 1 832.5 2 1207.0 3 1900.5 4 1474.5 5 1167.0 6 1631.5 7 3019.0 8 4779.5 9 5660.0 10 5349.5 11 5109.5 12 5688.0 13 6492.0 14 7501.5 15 8833.5 16 10174.0 17 11265.0 18 12540.0 19 13830.0 20 15091.5 21 17089.0 22 20131.5 23 24390.0 24 30127.5 25 37169.5 26 46544.0 27 57749.5 28 71579.0 29 90853.5 30 112803.5 31 133230.5 32 159034.0 33 184629.0 34 206131.0 35 230409.5 36 256909.0 37 289844.0 38 308243.5 39 305470.5 40 304093.0 41 306212.5 42 314125.5 43 327763.0 44 344455.5 45 371390.5 46 395230.0 47 414412.0 48 436233.5 49 440883.0 50 432279.0 51 426730.0 52 416115.0 53 398985.0 54 376676.5 55 340053.5 56 301429.5 57 261243.0 58 219609.0 59 187528.0 60 152247.5 61 117240.0 62 91033.0 63 69516.5 64 50339.5 65 34831.5 66 24796.0 67 18484.5 68 13834.0 69 10110.5 70 7538.5 71 5588.0 72 4138.5 73 3075.5 74 2286.0 75 1704.5 76 1167.0 77 697.5 78 443.0 79 297.0 80 201.5 81 126.0 82 75.5 83 51.5 84 39.5 85 25.5 86 16.5 87 8.5 88 4.5 89 3.5 90 2.5 91 2.5 92 2.0 93 2.0 94 2.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048626462145160636 2 0.0 3 0.0 4 0.014073024673380487 5 0.0034843802492551413 6 0.0 7 1.4518251038563086E-4 8 2.8068618674555304E-4 9 0.001761547792678988 10-11 5.952482925810867E-4 12-13 2.8068618674555304E-4 14-15 3.387591908998054E-5 16-17 8.227008921852417E-5 18-19 0.0 20-21 1.451825103856309E-5 22-23 0.0 24-25 1.1130659129565035E-4 26-27 1.451825103856309E-5 28-29 8.081826411466785E-4 30-31 7.936643901081154E-4 32-33 0.013734265482480681 34-35 0.022851727134698303 36-37 0.030585115521239572 38-39 0.020190047777628402 40-41 1.548613444113396E-4 42-43 0.0016260441163190658 44-45 0.008033432241338241 46-47 0.012205009706418702 48-49 0.00478134400870011 50-51 0.009069067482089075 52-53 0.013860090324814894 54-55 0.008875490801574902 56-57 0.002613285186941356 58-59 0.00824152717289098 60-61 0.0015147375250234155 62-63 7.452702199795719E-4 64-65 0.017078302638363047 66-67 0.04030266488305113 68-69 0.04908136734436895 70-71 0.04307565083141668 72-73 0.035685861052788065 74-75 0.02553760357683247 76-77 0.025610194832025288 78-79 0.0105934838411382 80-81 0.0017131536225504446 82-83 0.02425031865141321 84-85 0.0424271689516942 86-87 0.04298370190817245 88-89 0.0534223244048993 90-91 0.0301689256581341 92-93 0.02269686579028696 94-95 0.006194453776453584 96-97 0.003697314597820733 98-99 0.02218872700393725 100 0.018341390478718033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0331823E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.71310019615426 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95639382286198 44.00922260864093 2 14.490206583164111 17.01529901960577 3 4.80560810817526 8.464564510762361 4 2.0884491459891272 4.904772958521297 5 1.0857877325452157 3.18749819663412 6 0.6236173246048778 2.1968703878150313 7 0.4189404502420514 1.7218104841898476 8 0.28607661690739405 1.343715605780853 9 0.20411868182863419 1.078599655630038 >10 0.9681127015538339 9.998041753664717 >50 0.05056097094668869 2.0316478617126155 >100 0.020456442638092667 2.0620720296922546 >500 0.0010029411075982491 0.39077843967707 >1k 5.793471192779552E-4 0.6052885388277324 >5k 2.2282579022573545E-5 0.10836477398671733 >10k+ 6.684773706772064E-5 0.8814531748587564 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28038 0.27137514841282123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22220 0.21506369205124787 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17117 0.16567260201805625 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.935766805141745E-5 0.0 4 0.0 0.0 0.0 2.9036502077126176E-5 0.0 5 0.0 0.0 0.0 2.9036502077126176E-5 9.678834025708725E-6 6 9.678834025708725E-6 0.0 0.0 2.9036502077126176E-5 2.9036502077126176E-5 7 9.678834025708725E-6 0.0 0.0 2.9036502077126176E-5 2.9036502077126176E-5 8 9.678834025708725E-6 0.0 0.0 2.9036502077126176E-5 3.87153361028349E-5 9 9.678834025708725E-6 0.0 0.0 4.839417012854363E-5 6.775183817996108E-5 10-11 9.678834025708725E-6 0.0 0.0 5.323358714139799E-5 7.259125519281544E-5 12-13 9.678834025708725E-6 4.839417012854362E-6 0.0 8.227008921852415E-5 1.1130659129565034E-4 14-15 9.678834025708725E-6 1.4518251038563088E-5 0.0 9.678834025708726E-5 1.5002192739848523E-4 16-17 1.935766805141745E-5 1.935766805141745E-5 0.0 1.0646717428279598E-4 1.548613444113396E-4 18-19 1.935766805141745E-5 1.935766805141745E-5 0.0 1.7421901246275704E-4 2.0325551453988323E-4 20-21 1.935766805141745E-5 1.935766805141745E-5 0.0 3.3391977388695106E-4 2.1293434856559196E-4 22-23 1.935766805141745E-5 1.935766805141745E-5 0.0 9.8724107062229E-4 2.3713143362986375E-4 24-25 2.9036502077126176E-5 2.9036502077126176E-5 0.0 0.00231808074915724 2.6132851869413556E-4 26-27 2.9036502077126176E-5 3.87153361028349E-5 0.0 0.004336117643517509 2.6132851869413556E-4 28-29 2.9036502077126176E-5 3.87153361028349E-5 0.0 0.012185652038367285 2.8068618674555304E-4 30-31 2.9036502077126176E-5 3.87153361028349E-5 0.0 0.04208840976079439 2.8068618674555304E-4 32-33 2.9036502077126176E-5 3.87153361028349E-5 0.0 0.10963215300920273 2.903650207712618E-4 34-35 2.9036502077126176E-5 4.839417012854363E-5 0.0 0.2008938790376103 3.2908035687409667E-4 36-37 3.87153361028349E-5 7.259125519281544E-5 0.0 0.3482734847470771 3.3391977388695106E-4 38-39 3.87153361028349E-5 9.194892324423289E-5 0.0 0.598887534174753 3.484380249255141E-4 40-41 3.87153361028349E-5 9.678834025708726E-5 0.0 0.896923030911389 3.484380249255141E-4 42-43 3.87153361028349E-5 9.678834025708726E-5 0.0 1.189272212657921 3.629562759640772E-4 44-45 3.87153361028349E-5 9.678834025708726E-5 0.0 1.5142245468200528 3.8715336102834903E-4 46-47 3.87153361028349E-5 9.678834025708726E-5 0.0 1.8901407815445541 4.161898631054752E-4 48-49 3.87153361028349E-5 9.678834025708726E-5 0.0 2.2990763585477607 4.839417012854363E-4 50-51 3.87153361028349E-5 9.678834025708726E-5 0.0 2.7459723225997967 4.93620535311145E-4 52-53 3.87153361028349E-5 9.678834025708726E-5 0.0 3.193889403641545 5.274964544011256E-4 54-55 3.87153361028349E-5 9.678834025708726E-5 0.0 3.655289100481106 5.613723734911061E-4 56-57 3.87153361028349E-5 9.678834025708726E-5 0.0 4.153661943298874 5.710512075168149E-4 58-59 3.87153361028349E-5 9.678834025708726E-5 0.0 4.7144874626675275 5.807300415425235E-4 60-61 4.355475311568926E-5 9.678834025708726E-5 0.0 5.295788555417568 5.807300415425235E-4 62-63 4.839417012854363E-5 9.678834025708726E-5 0.0 5.889967336838813 5.904088755682322E-4 64-65 4.839417012854363E-5 9.678834025708726E-5 0.0 6.453009309199354 5.952482925810866E-4 66-67 4.839417012854363E-5 1.161460083085047E-4 0.0 7.048281798865505 6.049271266067954E-4 68-69 4.839417012854363E-5 1.161460083085047E-4 0.0 7.699009168081954 6.242847946582128E-4 70-71 5.323358714139799E-5 1.161460083085047E-4 0.0 8.377219586514403 6.484818797224845E-4 72-73 5.807300415425235E-5 1.161460083085047E-4 0.0 9.04406705380067 7.549490540052805E-4 74-75 5.807300415425235E-5 1.2582484233421344E-4 0.0 9.681563456903977 7.694673050438437E-4 76-77 5.807300415425235E-5 1.2582484233421344E-4 0.0 10.348962617729708 7.743067220566981E-4 78-79 5.807300415425235E-5 1.2582484233421344E-4 0.0 11.06037627628735 7.839855560824067E-4 80-81 6.291242116710671E-5 1.2582484233421344E-4 0.0 11.796340297351204 8.033432241338242E-4 82-83 6.775183817996108E-5 1.2582484233421344E-4 0.0 12.530281442103682 8.468979772495134E-4 84-85 6.775183817996108E-5 1.2582484233421344E-4 0.0 13.235079617604754 8.517373942623679E-4 86-87 6.775183817996108E-5 1.2582484233421344E-4 0.0 13.932899353773289 8.565768112752222E-4 88 6.775183817996108E-5 1.2582484233421344E-4 0.0 14.464281860035737 8.614162282880765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21550 0.0 37.26218 1 GTATCAA 33915 0.0 33.172523 1 TACCTGG 7080 0.0 30.66381 2 ATCAACG 41925 0.0 26.139044 3 CAACGCA 42035 0.0 26.112326 5 TATCAAC 43835 0.0 25.556574 2 GTACATG 33610 0.0 25.472355 1 TCAACGC 43515 0.0 25.238064 4 GTACTAG 4160 0.0 25.089228 1 AACGCAG 44565 0.0 24.934944 6 TACATGG 33990 0.0 24.580912 2 ACCTGGG 8915 0.0 24.142282 3 ACATGGG 34035 0.0 23.33432 3 ACGCAGA 49215 0.0 22.368969 7 CATGGGG 25905 0.0 22.004602 4 CGCAGAG 50055 0.0 21.84339 8 CTAACGC 1655 0.0 21.29597 3 ATGGGGG 18855 0.0 21.282131 5 TCTAACG 1680 0.0 21.257963 2 AGAGTAC 44135 0.0 21.152706 10-11 >>END_MODULE