FastQCFastQC Report
Mon 6 Jun 2016
ERR1211370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13144819
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407630.3101069706627379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294070.22371551863894054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202810.1542889255454944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209400.044.874831
GTATCAA378700.040.7913361
CAACGCA463100.032.86785
ATCAACG462950.032.8073733
TACCTGG96450.032.6972962
TATCAAC484700.031.6094682
AACGCAG495500.031.1935656
TCAACGC491000.031.1245024
GTACATG462550.028.964931
TACATGG460550.028.4941182
ACGCAGA542300.028.0855057
ACATGGG464300.027.4877833
CGCAGAG556300.027.292438
CATGGGG345750.025.7504434
GTACTAG58050.025.6530591
ACCTGGG122650.025.2189453
GCAGAGT604250.024.3249349
ATGGGGG264350.024.1662225
TGGGGGG281500.021.9595896
TAGGCAT75200.021.0659315
Produced by FastQC (version 0.11.5)