##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13144819 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.219409487494655 31.0 31.0 34.0 28.0 34.0 2 31.273802020400584 31.0 31.0 34.0 28.0 34.0 3 31.338807251739258 31.0 31.0 34.0 28.0 34.0 4 34.96212051303255 37.0 35.0 37.0 32.0 37.0 5 35.015009639919725 37.0 35.0 37.0 32.0 37.0 6 35.07247258406525 37.0 35.0 37.0 32.0 37.0 7 34.99441011702025 37.0 35.0 37.0 32.0 37.0 8 34.993136383239666 37.0 35.0 37.0 32.0 37.0 9 36.54561093614146 39.0 37.0 39.0 32.0 39.0 10-11 36.46496528404081 39.0 37.0 39.0 32.0 39.0 12-13 36.371018878236356 39.0 37.0 39.0 32.0 39.0 14-15 37.53310186317515 40.0 37.0 41.0 32.0 41.0 16-17 37.52676031522382 40.0 37.0 41.0 32.0 41.0 18-19 37.53144067636077 40.0 37.0 41.0 32.0 41.0 20-21 37.51742005728645 40.0 37.0 41.0 31.5 41.0 22-23 37.39034109180203 40.0 37.0 41.0 31.0 41.0 24-25 37.29724072275168 40.0 37.0 41.0 31.0 41.0 26-27 37.15692692307137 40.0 36.0 41.0 30.5 41.0 28-29 37.03355786032505 40.0 36.0 41.0 30.0 41.0 30-31 36.878329286998934 39.0 36.0 41.0 30.0 41.0 32-33 36.68732129365951 39.0 35.5 41.0 30.0 41.0 34-35 36.56635964329368 39.0 35.0 41.0 30.0 41.0 36-37 36.43331091131799 39.0 35.0 41.0 29.0 41.0 38-39 36.278311439663035 39.0 35.0 40.5 29.0 41.0 40-41 36.12063753026953 39.0 35.0 40.0 28.0 41.0 42-43 35.92980614643686 38.5 35.0 40.0 27.5 41.0 44-45 35.79232296770309 38.0 35.0 40.0 27.0 41.0 46-47 35.57254789890983 38.0 34.0 40.0 26.0 41.0 48-49 35.33320184933699 38.0 34.0 40.0 26.0 41.0 50-51 34.67988182264054 37.5 33.5 39.5 25.0 40.5 52-53 34.989403657821384 38.0 33.5 39.5 26.0 40.5 54-55 35.399614327135275 38.0 34.0 40.0 26.0 41.0 56-57 35.319364077968665 38.0 34.0 40.0 26.0 41.0 58-59 35.115602999174044 38.0 34.0 40.0 26.0 41.0 60-61 34.89880005194442 38.0 34.0 40.0 26.0 41.0 62-63 34.625776779429216 37.0 33.0 40.0 25.0 41.0 64-65 34.42287212170818 37.0 33.0 40.0 25.0 41.0 66-67 34.225210860644026 36.0 33.0 39.5 25.0 41.0 68-69 33.900726818680425 36.0 33.0 39.0 24.0 41.0 70-71 33.55053002251306 35.0 32.5 39.0 23.5 41.0 72-73 33.17920653757196 35.0 32.0 38.5 23.0 40.0 74-75 32.80297667088455 35.0 32.0 37.5 22.0 40.0 76-77 32.4381148953059 35.0 31.0 37.0 21.5 39.0 78-79 32.07508578094533 35.0 31.0 36.5 20.5 39.0 80-81 31.7145349053494 35.0 31.0 36.0 20.0 38.5 82-83 31.378719174451927 35.0 30.5 35.5 19.5 37.0 84-85 31.06749309366679 34.0 30.0 35.0 19.0 37.0 86-87 30.769084153992534 34.0 30.0 35.0 18.0 36.5 88-89 30.5008981485405 34.0 29.5 35.0 18.0 36.0 90-91 30.246979931789095 34.0 29.0 35.0 17.0 36.0 92-93 30.000601872114025 34.0 29.0 35.0 16.0 35.5 94-95 29.757849993978617 34.0 29.0 35.0 15.0 35.0 96-97 29.50944307411156 34.0 28.5 35.0 12.0 35.0 98-99 29.25413225545365 34.0 27.0 35.0 10.0 35.0 100 28.05144840716331 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.29176740302331083 1101 2 -0.3255085186747593 1101 3 -0.4021833004082467 1101 4 -0.2689294796658288 1101 5 -0.2685583663864648 1101 6 -0.2790740547905841 1101 7 -0.3078471593354948 1101 8 -0.317385172305535 1101 9 -0.5101314384369928 1101 10-11 -0.4221470813600732 1101 12-13 -0.3880257502321314 1101 14-15 -0.591918924080737 1101 16-17 -0.5972193269714197 1101 18-19 -0.6286973842488379 1101 20-21 -0.6613516096026402 1101 22-23 -0.6315597440030913 1101 24-25 -0.6629701000496198 1101 26-27 -0.7782346435812642 1101 28-29 -0.7048164706440119 1101 30-31 -0.7153251007672168 1101 32-33 -0.7031009558011618 1101 34-35 -0.7470751532065663 1101 36-37 -0.8029814407992362 1101 38-39 -0.8167615899558456 1101 40-41 -0.9761906779891305 1101 42-43 -0.9707029868711103 1101 44-45 -1.0397978798809078 1101 46-47 -1.0028136456421564 1101 48-49 -1.0383356550409175 1101 50-51 -0.9070181507592778 1101 52-53 -0.7549784280980347 1101 54-55 -0.7585892883775145 1101 56-57 -0.7894505896376316 1101 58-59 -0.7094341058469595 1101 60-61 -0.732345204243174 1101 62-63 -0.8425405836044533 1101 64-65 -0.8335242936882707 1101 66-67 -0.8788874089539078 1101 68-69 -0.9110665689285824 1101 70-71 -0.8899433993631689 1101 72-73 -0.9221165625312366 1101 74-75 -1.003040558571012 1101 76-77 -0.9682309478626863 1101 78-79 -0.9027622733114207 1101 80-81 -1.025034949261844 1101 82-83 -1.0439553958469006 1101 84-85 -1.0501944010913817 1101 86-87 -1.1764879569977857 1101 88-89 -1.268283937113587 1101 90-91 -1.3605632256580762 1101 92-93 -1.4103283279722625 1101 94-95 -1.4332075463865657 1101 96-97 -1.6007956636704996 1101 98-99 -1.7142543439220788 1101 100 -1.6668242534372588 1102 1 -0.4197458359624413 1102 2 -0.3960601584420864 1102 3 -0.40508445832783124 1102 4 -0.4093555833474767 1102 5 -0.3579500504365285 1102 6 -0.3216319130591998 1102 7 -0.41977215757105313 1102 8 -0.39409465196182936 1102 9 -0.5321257327995141 1102 10-11 -0.4214820931845722 1102 12-13 -0.5449307849727916 1102 14-15 -0.743761284639497 1102 16-17 -0.7465221039717207 1102 18-19 -0.7225420776556959 1102 20-21 -0.8019922401506534 1102 22-23 -0.7528812002178213 1102 24-25 -0.7719102885790576 1102 26-27 -0.8305682894203699 1102 28-29 -0.8881908023220362 1102 30-31 -0.8136214146104876 1102 32-33 -0.8967667523045861 1102 34-35 -0.9356352625233839 1102 36-37 -0.9270966727892258 1102 38-39 -1.032833315672569 1102 40-41 -1.1865633823906094 1102 42-43 -1.103433272602338 1102 44-45 -1.0387949369050062 1102 46-47 -1.1880841583462498 1102 48-49 -1.2360242002717712 1102 50-51 -1.0765872152174865 1102 52-53 -0.8257487891921684 1102 54-55 -0.8678801971124344 1102 56-57 -0.8600643232476415 1102 58-59 -0.8439180005196576 1102 60-61 -0.9603604107292583 1102 62-63 -0.9116427207263627 1102 64-65 -0.9759336134501524 1102 66-67 -1.0192667008742191 1102 68-69 -0.9134410885975655 1102 70-71 -0.9653760892182603 1102 72-73 -0.9272542265431483 1102 74-75 -1.0285685315305777 1102 76-77 -1.0051380685955777 1102 78-79 -1.105303520408718 1102 80-81 -1.1341652022477966 1102 82-83 -1.2623276723184453 1102 84-85 -1.3311151206132408 1102 86-87 -1.2433844766671847 1102 88-89 -1.366606486119803 1102 90-91 -1.2877167253567947 1102 92-93 -1.3514255437416551 1102 94-95 -1.4454128524993202 1102 96-97 -1.5492650710250544 1102 98-99 -1.58964579589977 1102 100 -1.61747310243695 1103 1 -0.4976407962816758 1103 2 -0.49408366134724346 1103 3 -0.5431118194073292 1103 4 -0.3668728207141214 1103 5 -0.39041255308040945 1103 6 -0.39554457084730643 1103 7 -0.4398502316260462 1103 8 -0.41266278685716884 1103 9 -0.5321369395578941 1103 10-11 -0.5602833762257617 1103 12-13 -0.6629550962892807 1103 14-15 -0.7404618167577368 1103 16-17 -0.7935360868347416 1103 18-19 -0.8399888826043096 1103 20-21 -0.813088127858876 1103 22-23 -0.8360241482606696 1103 24-25 -0.8416075432617518 1103 26-27 -0.9213912823463559 1103 28-29 -0.8951853214090804 1103 30-31 -0.9158481456597443 1103 32-33 -0.9418871970546334 1103 34-35 -0.9548756733195418 1103 36-37 -0.9462038603862766 1103 38-39 -1.0095808167636662 1103 40-41 -0.9908645103363583 1103 42-43 -1.002812073599351 1103 44-45 -1.1582516015443929 1103 46-47 -1.1611332233336142 1103 48-49 -1.0630315243381645 1103 50-51 -0.9910572505106927 1103 52-53 -0.8506088785315313 1103 54-55 -0.8410106902537109 1103 56-57 -0.8087899501491265 1103 58-59 -0.7743634528506504 1103 60-61 -0.8441325351573994 1103 62-63 -0.8873388220430201 1103 64-65 -0.8090950183379135 1103 66-67 -0.859822546026642 1103 68-69 -0.8439295001690255 1103 70-71 -0.9040195368843129 1103 72-73 -0.9020873769699804 1103 74-75 -0.9026065266192447 1103 76-77 -0.9749855761759214 1103 78-79 -0.9796559603654984 1103 80-81 -0.976181486231873 1103 82-83 -0.9887897898767619 1103 84-85 -1.037967446520291 1103 86-87 -1.1601736781824492 1103 88-89 -1.1541983830632496 1103 90-91 -1.1209062157533154 1103 92-93 -1.1545245143299994 1103 94-95 -1.2002711957189938 1103 96-97 -1.337205864089075 1103 98-99 -1.4246821372063394 1103 100 -1.4152653832339759 1104 1 -0.4290275305035749 1104 2 -0.4379090783838535 1104 3 -0.4314981274216265 1104 4 -0.32227072671452106 1104 5 -0.3380255025256318 1104 6 -0.2696301960433516 1104 7 -0.314204784072615 1104 8 -0.35922776180750304 1104 9 -0.45520626785116747 1104 10-11 -0.42204788013251004 1104 12-13 -0.4989861310256458 1104 14-15 -0.7295224365929585 1104 16-17 -0.6933617050129826 1104 18-19 -0.6987603524800292 1104 20-21 -0.7782753512891318 1104 22-23 -0.7239498795921548 1104 24-25 -0.7555597725667553 1104 26-27 -0.761897186783699 1104 28-29 -0.827685567298083 1104 30-31 -0.8676226556982201 1104 32-33 -0.829487424513033 1104 34-35 -0.7965591424013283 1104 36-37 -0.8928835392330896 1104 38-39 -0.914702640365114 1104 40-41 -0.8852686193852506 1104 42-43 -0.995304262746906 1104 44-45 -1.019017286604445 1104 46-47 -0.9382731997459928 1104 48-49 -0.9336228896025105 1104 50-51 -0.8654721454474199 1104 52-53 -0.7521399806104654 1104 54-55 -0.6666772488178268 1104 56-57 -0.698027776206807 1104 58-59 -0.7641013639726353 1104 60-61 -0.776409908141332 1104 62-63 -0.7691169960491493 1104 64-65 -0.7511398224153325 1104 66-67 -0.7328009286175288 1104 68-69 -0.808892156053787 1104 70-71 -0.741859384282499 1104 72-73 -0.7447982411878584 1104 74-75 -0.8703496665376136 1104 76-77 -0.8695211693842495 1104 78-79 -0.8076752004045531 1104 80-81 -0.8652060423468448 1104 82-83 -0.9565030576042517 1104 84-85 -0.9965137396660566 1104 86-87 -0.9598104381008561 1104 88-89 -0.9116706286190812 1104 90-91 -0.9148724540857742 1104 92-93 -0.9897872234685607 1104 94-95 -1.0151929476360415 1104 96-97 -1.0699058892539277 1104 98-99 -1.1877926397237104 1104 100 -1.1253138920390207 1105 1 -0.5016316736018851 1105 2 -0.495151463887165 1105 3 -0.5088595727314278 1105 4 -0.3229776555172279 1105 5 -0.42466279316335687 1105 6 -0.4025920203968383 1105 7 -0.38688237441236595 1105 8 -0.3887789600908178 1105 9 -0.5520254036785914 1105 10-11 -0.47896306874961425 1105 12-13 -0.6175629242208629 1105 14-15 -0.7571190170270441 1105 16-17 -0.7272920794655917 1105 18-19 -0.73344968534537 1105 20-21 -0.7251997399887102 1105 22-23 -0.6766457518070013 1105 24-25 -0.701073149786339 1105 26-27 -0.7715492189172437 1105 28-29 -0.7615786015889441 1105 30-31 -0.8006163286969112 1105 32-33 -0.8394749123148415 1105 34-35 -0.8235221803369512 1105 36-37 -0.8612726888853715 1105 38-39 -0.9842717778414354 1105 40-41 -0.8798629454299132 1105 42-43 -0.8900034832096466 1105 44-45 -0.8693467740122429 1105 46-47 -0.8732296561779052 1105 48-49 -0.9086458557555588 1105 50-51 -0.8787994108700445 1105 52-53 -0.7360008759083243 1105 54-55 -0.6965751259667243 1105 56-57 -0.7125950346668262 1105 58-59 -0.6267566749573561 1105 60-61 -0.628012470313756 1105 62-63 -0.7048511437041256 1105 64-65 -0.6357538333243795 1105 66-67 -0.6600286526319721 1105 68-69 -0.6377446377174252 1105 70-71 -0.6583880963110929 1105 72-73 -0.6160353049384852 1105 74-75 -0.639854303786386 1105 76-77 -0.7091023896307647 1105 78-79 -0.7546286884065232 1105 80-81 -0.8380442631298983 1105 82-83 -0.8612387020200956 1105 84-85 -0.8250078246001742 1105 86-87 -0.7977910461651554 1105 88-89 -0.8321240723161232 1105 90-91 -0.896903279989786 1105 92-93 -0.9492630682516356 1105 94-95 -0.9743738886611659 1105 96-97 -1.0121300738927665 1105 98-99 -0.9709020288692045 1105 100 -0.9744740986462155 1106 1 -0.43661971226940466 1106 2 -0.4717008606263171 1106 3 -0.5111045571004098 1106 4 -0.34768046604247616 1106 5 -0.3545240084275392 1106 6 -0.36897338509279365 1106 7 -0.4098409316951077 1106 8 -0.39743836218834616 1106 9 -0.5920647808483324 1106 10-11 -0.7347223058686652 1106 12-13 -0.6044683766277075 1106 14-15 -0.8001566676230212 1106 16-17 -0.7296958418381294 1106 18-19 -0.8079358646603438 1106 20-21 -0.8063406261369224 1106 22-23 -0.7306375387484536 1106 24-25 -0.7860160509789225 1106 26-27 -0.7622071894549691 1106 28-29 -0.790955413243438 1106 30-31 -0.840755444536434 1106 32-33 -0.9076659611816993 1106 34-35 -0.8585950580071184 1106 36-37 -0.7872270068038532 1106 38-39 -0.9170645398580248 1106 40-41 -0.9120850426059945 1106 42-43 -0.886242575056805 1106 44-45 -0.8921859737463649 1106 46-47 -0.9142625412414063 1106 48-49 -0.8720475821494347 1106 50-51 -0.8976799396556103 1106 52-53 -0.7999856146691826 1106 54-55 -0.747851750022015 1106 56-57 -0.8282381224017783 1106 58-59 -0.766115206763665 1106 60-61 -0.7504035529318287 1106 62-63 -0.7902266439855339 1106 64-65 -0.7483598455382321 1106 66-67 -0.742314123283812 1106 68-69 -0.7183438685449488 1106 70-71 -0.7293528133596467 1106 72-73 -0.7498092441867499 1106 74-75 -0.7577893853098523 1106 76-77 -0.7539412950254949 1106 78-79 -0.7422890179317321 1106 80-81 -0.7785275272706116 1106 82-83 -0.7562311265470001 1106 84-85 -0.7956531052648188 1106 86-87 -0.795302638730174 1106 88-89 -0.8191207047271405 1106 90-91 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84-85 0.7303421702631709 2116 86-87 0.7886528764310441 2116 88-89 0.7848055796663154 2116 90-91 0.8465785851476824 2116 92-93 0.9050680125869164 2116 94-95 1.0135173789040195 2116 96-97 1.0633866239445169 2116 98-99 1.0994421980891929 2116 100 0.9639038513710503 2201 1 0.7208778175120223 2201 2 0.7401890375998512 2201 3 0.8219327568508561 2201 4 0.6567537908119832 2201 5 0.6495129302762592 2201 6 0.6522251373370267 2201 7 0.6830275860516934 2201 8 0.7120710822737948 2201 9 0.9010647903866911 2201 10-11 0.9215216688582402 2201 12-13 0.5849418098773427 2201 14-15 0.8646091023377096 2201 16-17 1.004280132229134 2201 18-19 1.084458023311818 2201 20-21 1.0929675910668522 2201 22-23 1.0958121585895455 2201 24-25 1.0742715456009577 2201 26-27 1.065941319434188 2201 28-29 1.0592512504688472 2201 30-31 1.0742629452508865 2201 32-33 1.064509734932635 2201 34-35 1.0355521299448824 2201 36-37 0.5215383276165255 2201 38-39 0.8572253921762112 2201 40-41 0.9485219020509206 2201 42-43 0.9939164123127924 2201 44-45 0.9755197578122932 2201 46-47 0.9834411007146926 2201 48-49 0.9795285076948446 2201 50-51 1.10658103252441 2201 52-53 1.0891666018191444 2201 54-55 1.0345741762285101 2201 56-57 1.0026995401744898 2201 58-59 0.9750970715280758 2201 60-61 0.9373431305238498 2201 62-63 0.8723757977017499 2201 64-65 0.8916492617150951 2201 66-67 0.9031767149056051 2201 68-69 0.25913526089560435 2201 70-71 0.6551776487233454 2201 72-73 0.7226041361890978 2201 74-75 0.7192606850364243 2201 76-77 0.6653244276278016 2201 78-79 0.6368276230041587 2201 80-81 0.5995118028672302 2201 82-83 0.6149953719627952 2201 84-85 0.604155120702984 2201 86-87 0.5985768102901758 2201 88-89 0.512824798768424 2201 90-91 0.5134620051234045 2201 92-93 0.48829913635831446 2201 94-95 0.4600411719261217 2201 96-97 0.428029255030804 2201 98-99 0.3581638772424327 2201 100 0.3642596315743525 2202 1 0.7853619481651464 2202 2 0.8061651874822715 2202 3 0.8906570434263301 2202 4 0.6929799014654989 2202 5 0.7168900936116813 2202 6 0.7219309390411226 2202 7 0.7686084142385994 2202 8 0.7960428649356217 2202 9 0.9724195269401577 2202 10-11 0.968384954671329 2202 12-13 1.0095527700899893 2202 14-15 1.2335930129391315 2202 16-17 1.1367109057597489 2202 18-19 1.1533552076538527 2202 20-21 1.1536328472540447 2202 22-23 0.669652118922123 2202 24-25 0.8512122272698903 2202 26-27 1.0629957834194101 2202 28-29 1.1409265571807694 2202 30-31 1.1677008476113286 2202 32-33 1.1653319833485583 2202 34-35 1.149820184619145 2202 36-37 1.1364564329845663 2202 38-39 1.0838501160549114 2202 40-41 1.0671860376386988 2202 42-43 0.3702432606810575 2202 44-45 0.8502542692631678 2202 46-47 1.0017069110200865 2202 48-49 0.95014698242219 2202 50-51 1.0979448617304755 2202 52-53 1.0373228961167769 2202 54-55 0.9901356858307437 2202 56-57 0.8866002749305579 2202 58-59 0.8924057758013149 2202 60-61 0.8728485652461586 2202 62-63 0.8360462653895553 2202 64-65 0.8774359822156157 2202 66-67 0.8454868456844693 2202 68-69 0.8290842950192072 2202 70-71 0.7970888730508605 2202 72-73 0.7273470032646614 2202 74-75 0.6578702224417796 2202 76-77 0.6188828195227174 2202 78-79 0.6243189516990597 2202 80-81 0.5883730392672142 2202 82-83 0.5683364859157614 2202 84-85 0.537946173025631 2202 86-87 0.5776427079822462 2202 88-89 0.535641756433435 2202 90-91 0.5570500075769935 2202 92-93 0.4776494792744188 2202 94-95 0.45004447138082426 2202 96-97 0.4510848882223151 2202 98-99 0.44503793845643 2202 100 0.4493613916181438 2203 1 -0.13637870220433612 2203 2 0.057993381856071835 2203 3 0.469268412706743 2203 4 0.49755461682229907 2203 5 0.5435408495411593 2203 6 0.6065820884729192 2203 7 0.6595374246632986 2203 8 0.7151843468725829 2203 9 0.82934780666546 2203 10-11 0.8554170990830201 2203 12-13 0.912761020575374 2203 14-15 1.0903279999498423 2203 16-17 1.0613165046678645 2203 18-19 1.0562448965405693 2203 20-21 1.0524899093100117 2203 22-23 1.0696816259989035 2203 24-25 1.0491562429647558 2203 26-27 1.064826673653478 2203 28-29 1.03660621443003 2203 30-31 1.0810446045334672 2203 32-33 1.0654186981659635 2203 34-35 1.0181880698637826 2203 36-37 0.9769257675180896 2203 38-39 1.0072969684570765 2203 40-41 0.9834598308676377 2203 42-43 1.0098844987685993 2203 44-45 1.0155104032267914 2203 46-47 0.9836620420733126 2203 48-49 1.0075497834601848 2203 50-51 1.1490635611855495 2203 52-53 1.10984000741351 2203 54-55 1.0159411435802568 2203 56-57 0.9978487173716388 2203 58-59 0.917724278632086 2203 60-61 0.9267012774522883 2203 62-63 0.879898054286862 2203 64-65 0.9385007409582329 2203 66-67 0.9193451381347941 2203 68-69 0.9077222171239896 2203 70-71 0.8684103493315547 2203 72-73 0.769378361212766 2203 74-75 0.7625099430118567 2203 76-77 0.7066723546819702 2203 78-79 0.6673665766732171 2203 80-81 0.591723762702717 2203 82-83 0.6208253796710892 2203 84-85 0.6360585442612212 2203 86-87 0.6576358315796433 2203 88-89 0.6582581635397524 2203 90-91 0.6083791030677403 2203 92-93 0.6214606021923856 2203 94-95 0.6405518189433117 2203 96-97 0.6314324526133142 2203 98-99 0.5335008789877485 2203 100 0.570534158556395 2204 1 0.8011225731449301 2204 2 0.8358288119342774 2204 3 0.8585088503562233 2204 4 0.7534080146945001 2204 5 0.6974467310852788 2204 6 0.740111568236685 2204 7 0.7958782357051035 2204 8 0.8096907085018117 2204 9 0.9526288915482652 2204 10-11 0.992852827092797 2204 12-13 1.0175462607394152 2204 14-15 1.2431082064693655 2204 16-17 1.2202603213979373 2204 18-19 1.2110108062763274 2204 20-21 1.161302858367037 2204 22-23 1.2362394212853545 2204 24-25 1.23951139449521 2204 26-27 1.2129832432874963 2204 28-29 1.1805920364876101 2204 30-31 1.1933584340991032 2204 32-33 1.2239927945738387 2204 34-35 1.2212769820009584 2204 36-37 1.1270316445819546 2204 38-39 1.1013938463072819 2204 40-41 1.151469815536501 2204 42-43 1.1478567091513057 2204 44-45 1.1670360651997882 2204 46-47 1.198497189117532 2204 48-49 1.1065256673975838 2204 50-51 1.152674377424546 2204 52-53 1.164875500414766 2204 54-55 1.0756914250800875 2204 56-57 1.0629133324900621 2204 58-59 1.0732698664375064 2204 60-61 1.0533190003093793 2204 62-63 0.9915982693537799 2204 64-65 1.0070950987681755 2204 66-67 0.9588721346033253 2204 68-69 0.9352844792386179 2204 70-71 0.9224433741546747 2204 72-73 0.9203952056017926 2204 74-75 0.8937089948355137 2204 76-77 0.8677034036554332 2204 78-79 0.8796808602507298 2204 80-81 0.8932616805015279 2204 82-83 0.8695228021658998 2204 84-85 0.903646876635225 2204 86-87 0.8528287788894708 2204 88-89 0.827380277714358 2204 90-91 0.8528896131680348 2204 92-93 0.8922400891779354 2204 94-95 0.8411283990455516 2204 96-97 0.8786363756350504 2204 98-99 0.81994876448735 2204 100 0.8903701016181209 2205 1 0.7722917932115863 2205 2 0.8024270977681169 2205 3 0.8732020213311955 2205 4 0.699765619878292 2205 5 0.7210430821723008 2205 6 0.7284550638740868 2205 7 0.813175769569412 2205 8 0.8105182917109417 2205 9 1.022683907888478 2205 10-11 1.0067493992275232 2205 12-13 1.0663672288750945 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1.0843675939301782 2205 82-83 1.0723362644501258 2205 84-85 1.041636309207945 2205 86-87 1.0041539601253184 2205 88-89 1.0261849104215095 2205 90-91 1.0724839017999805 2205 92-93 1.0445294970932615 2205 94-95 1.079882968457138 2205 96-97 1.1111528727110844 2205 98-99 1.081513071002469 2205 100 1.0817501245032872 2206 1 0.7547575151674124 2206 2 0.8106730253034584 2206 3 0.8332788575586605 2206 4 0.7184955110463065 2206 5 0.7398664642287827 2206 6 0.7456483387299357 2206 7 0.807704838118056 2206 8 0.7976138301858171 2206 9 0.9360720117985295 2206 10-11 0.9857428875962313 2206 12-13 0.9919645743469161 2206 14-15 1.193468423687115 2206 16-17 1.1932667676636655 2206 18-19 1.2181374164034011 2206 20-21 1.1600494724022425 2206 22-23 1.2252566142420775 2206 24-25 1.234764154326534 2206 26-27 1.244612026657009 2206 28-29 1.2206631976994018 2206 30-31 1.2827139334981226 2206 32-33 1.2505767512196329 2206 34-35 1.3080264280953386 2206 36-37 1.2523120886553443 2206 38-39 1.2837846064923681 2206 40-41 1.2902506565276042 2206 42-43 1.274084909520603 2206 44-45 1.3264607029111914 2206 46-47 1.3433446483030593 2206 48-49 1.3586356969354654 2206 50-51 1.3645354834107053 2206 52-53 1.286198896967207 2206 54-55 1.2526710180812088 2206 56-57 1.1980462057699768 2206 58-59 1.1957850204345633 2206 60-61 1.1631579666073435 2206 62-63 1.1422784968778004 2206 64-65 1.1654477945922395 2206 66-67 1.131318119119861 2206 68-69 1.1203571979920497 2206 70-71 1.1165142745929657 2206 72-73 1.1007226278669222 2206 74-75 1.1031769670955143 2206 76-77 1.0711795894151734 2206 78-79 1.020904067655792 2206 80-81 1.0019035150137192 2206 82-83 0.9881438336705735 2206 84-85 0.9957057085932774 2206 86-87 1.004819837007421 2206 88-89 1.0221071140009705 2206 90-91 1.0667205028463798 2206 92-93 0.9951222987710935 2206 94-95 1.0442039528883988 2206 96-97 1.0339899639936796 2206 98-99 1.065009799081139 2206 100 1.1461024912030062 2207 1 0.7175009193426014 2207 2 0.7237462507327095 2207 3 0.8131232304969558 2207 4 0.6721979094064494 2207 5 0.7197077329266861 2207 6 0.6869754855351218 2207 7 0.7573982214896873 2207 8 0.765694606979956 2207 9 0.9872031729868169 2207 10-11 0.9361440806535697 2207 12-13 0.9790165647138238 2207 14-15 1.1792128256783556 2207 16-17 1.1827114928046143 2207 18-19 1.1891145781123669 2207 20-21 1.1835899306018476 2207 22-23 1.1927287798390722 2207 24-25 1.2322398752220423 2207 26-27 1.2477740042056311 2207 28-29 1.2107616312756946 2207 30-31 1.2219096248124615 2207 32-33 1.2609982264575663 2207 34-35 1.2664878070572385 2207 36-37 1.3441439918085294 2207 38-39 1.2953776752209336 2207 40-41 1.277905152513874 2207 42-43 1.3195016700862539 2207 44-45 1.3625990004222714 2207 46-47 1.3937673052104103 2207 48-49 1.414229469520528 2207 50-51 1.426341509890392 2207 52-53 1.30216778699031 2207 54-55 1.2729468787190044 2207 56-57 1.231552707695812 2207 58-59 1.2374598850399465 2207 60-61 1.1867191854864814 2207 62-63 1.199666297453163 2207 64-65 1.231848980103365 2207 66-67 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2311 54-55 1.1797886495815888 2311 56-57 1.1643410389364917 2311 58-59 1.1674267465157584 2311 60-61 1.1859555859619917 2311 62-63 1.1163739570835673 2311 64-65 1.1068374159072505 2311 66-67 1.1603164645973791 2311 68-69 1.1940061751552093 2311 70-71 1.169751518167132 2311 72-73 1.1460109550657975 2311 74-75 1.1807561559981465 2311 76-77 1.1401422438993478 2311 78-79 1.1190502385063184 2311 80-81 1.1475120904408591 2311 82-83 1.1772785392138552 2311 84-85 1.1527071578119035 2311 86-87 1.1987600522365511 2311 88-89 1.2774260756978535 2311 90-91 1.3033003591556636 2311 92-93 1.3193825305429137 2311 94-95 1.382670697933559 2311 96-97 1.4383249828536435 2311 98-99 1.4525917779346749 2311 100 1.5566506110703564 2312 1 0.5719113950253494 2312 2 0.6002883782169768 2312 3 0.6644418588777938 2312 4 0.5562178287263748 2312 5 0.575673088183585 2312 6 0.45355283201898544 2312 7 0.5805447814017484 2312 8 0.5965072014590547 2312 9 0.7853759222404264 2312 10-11 0.8283579878959273 2312 12-13 0.8724261718740394 2312 14-15 1.0932437742513557 2312 16-17 1.0721224654400956 2312 18-19 1.0481464364186195 2312 20-21 1.0729105191177837 2312 22-23 1.1232354117001933 2312 24-25 1.1370931152494776 2312 26-27 1.1195040400892537 2312 28-29 1.162628459795421 2312 30-31 1.042238216960861 2312 32-33 1.1860559366084757 2312 34-35 1.1815939125255497 2312 36-37 1.2097107953526773 2312 38-39 1.2780656231812273 2312 40-41 1.2875603026713733 2312 42-43 1.3582227639182705 2312 44-45 1.3316697533602024 2312 46-47 1.3009733436978408 2312 48-49 1.345463946902349 2312 50-51 1.3015427488110518 2312 52-53 1.1622171442277462 2312 54-55 1.1408134522305957 2312 56-57 1.089850197135057 2312 58-59 1.1166040364820518 2312 60-61 1.1258189078057228 2312 62-63 1.1162698686309014 2312 64-65 1.0616492185733364 2312 66-67 1.087978706258447 2312 68-69 1.0902946769061401 2312 70-71 1.0829913029200853 2312 72-73 1.1556804660532478 2312 74-75 1.1674434993685097 2312 76-77 1.0831380255988066 2312 78-79 1.0958846711191725 2312 80-81 0.9617730318005222 2312 82-83 1.088064854310499 2312 84-85 1.1269570162952647 2312 86-87 1.1756195566294387 2312 88-89 1.2289873033044962 2312 90-91 1.2608479239929977 2312 92-93 1.2900901363934665 2312 94-95 1.3157324149908192 2312 96-97 1.2805428865887336 2312 98-99 1.243062046426445 2312 100 1.4159164412922536 2313 1 0.5849811929641788 2313 2 0.5885107723248382 2313 3 0.6367223419460437 2313 4 0.476974233675989 2313 5 0.5106562062042102 2313 6 0.5557662827265659 2313 7 0.5924478393551666 2313 8 0.6414363448129237 2313 9 0.7975409057341807 2313 10-11 0.6824255520196587 2313 12-13 0.8024966068736035 2313 14-15 1.004729286524416 2313 16-17 0.982970534398163 2313 18-19 0.98380184464461 2313 20-21 1.041895309300756 2313 22-23 1.0665394925844396 2313 24-25 1.0949258382589093 2313 26-27 1.1024959126971652 2313 28-29 1.1153628247095781 2313 30-31 1.1571489921533384 2313 32-33 1.178396059384788 2313 34-35 1.1716347103303377 2313 36-37 1.185955521229836 2313 38-39 1.2172444134345923 2313 40-41 1.245811615709691 2313 42-43 1.2428022766426636 2313 44-45 1.2462337494912106 2313 46-47 1.2429639711297398 2313 48-49 1.3247114149147592 2313 50-51 1.255583176152939 2313 52-53 1.1353400575860206 2313 54-55 1.1029184520243334 2313 56-57 1.1290218381186747 2313 58-59 1.1363340389334624 2313 60-61 1.086980587381234 2313 62-63 1.130311276992927 2313 64-65 1.0881075442483876 2313 66-67 1.0720211706904408 2313 68-69 1.0762609200453852 2313 70-71 1.054027822819343 2313 72-73 1.1175891600699401 2313 74-75 1.1208732229503866 2313 76-77 1.0881497100924449 2313 78-79 1.133826247804457 2313 80-81 1.117186988275595 2313 82-83 1.1592790046257697 2313 84-85 1.2208563416510003 2313 86-87 1.2862928001792966 2313 88-89 1.2900409396121013 2313 90-91 1.3287391168447868 2313 92-93 1.4166380432406385 2313 94-95 1.3789427124007645 2313 96-97 1.380891357120376 2313 98-99 1.4328699895117758 2313 100 1.5725743365166345 2314 1 0.4522106340076064 2314 2 0.4379383035958675 2314 3 0.5033386564170463 2314 4 0.38740606087858254 2314 5 0.4324917269100439 2314 6 0.45597341522258716 2314 7 0.48564239286731237 2314 8 0.4873577270627294 2314 9 0.6325347902011771 2314 10-11 0.5734181428075757 2314 12-13 0.3543937088166871 2314 14-15 0.730886855108956 2314 16-17 0.7360969348911794 2314 18-19 0.7788946294843768 2314 20-21 0.8421601134531116 2314 22-23 0.7668832356592361 2314 24-25 0.7809216778534918 2314 26-27 0.8931110249636021 2314 28-29 0.9449056588377118 2314 30-31 0.8695833586169286 2314 32-33 0.8315529925922007 2314 34-35 0.8749874765695367 2314 36-37 0.9479241598506007 2314 38-39 0.9951915957718782 2314 40-41 0.9993502134658456 2314 42-43 1.075878258263252 2314 44-45 1.0946357040793941 2314 46-47 1.0893034785068139 2314 48-49 1.1408118478792133 2314 50-51 1.0751266305997476 2314 52-53 0.9189268019603389 2314 54-55 0.8982519755737428 2314 56-57 0.9151992838711323 2314 58-59 0.9374357956939221 2314 60-61 0.8909496604498273 2314 62-63 0.8638734221358106 2314 64-65 0.8608480671950076 2314 66-67 0.8978583592893372 2314 68-69 0.8929360467949152 2314 70-71 0.9268181342058313 2314 72-73 0.9389434505849508 2314 74-75 0.9368686400090525 2314 76-77 0.9887463678783703 2314 78-79 1.0414720350540279 2314 80-81 1.0689434172268584 2314 82-83 0.9340433734312903 2314 84-85 0.9407262163241512 2314 86-87 1.135811890261703 2314 88-89 1.2404396910184943 2314 90-91 1.2756331801253822 2314 92-93 1.2966900443751896 2314 94-95 1.2972162891177774 2314 96-97 1.3716888958936657 2314 98-99 1.4499210271296086 2314 100 1.400734039806526 2315 1 0.3864564054420576 2315 2 0.42575724685231364 2315 3 0.4923278500067667 2315 4 0.3905079262314004 2315 5 0.37823025127832466 2315 6 -0.3018726442964663 2315 7 0.20521298916111164 2315 8 0.27132474650520066 2315 9 0.5058212060207268 2315 10-11 0.6349046760724022 2315 12-13 0.6404157716449319 2315 14-15 0.7816049093574193 2315 16-17 0.6752592176977217 2315 18-19 0.793250500266673 2315 20-21 0.8050195841723564 2315 22-23 0.6823571713238223 2315 24-25 0.7925647861489509 2315 26-27 0.8142058541835695 2315 28-29 0.9435331447441584 2315 30-31 0.945305976446356 2315 32-33 0.9861653565470618 2315 34-35 0.9821040448712139 2315 36-37 1.0140316612570288 2315 38-39 1.046533157828982 2315 40-41 1.0635628326325914 2315 42-43 1.062869048545977 2315 44-45 0.9972763426868596 2315 46-47 1.0797866689641609 2315 48-49 1.095735855228753 2315 50-51 1.0480106948489905 2315 52-53 0.9151678712254068 2315 54-55 0.7384411605966292 2315 56-57 0.7589395948222091 2315 58-59 0.6986393246805633 2315 60-61 0.6833899360627242 2315 62-63 0.7797230282981502 2315 64-65 0.8614181256960052 2315 66-67 0.863200701326555 2315 68-69 0.9207211728580802 2315 70-71 0.9453009639348195 2315 72-73 0.9411973646182972 2315 74-75 0.9444823335660217 2315 76-77 0.9135837226002295 2315 78-79 0.8537907525595259 2315 80-81 0.8702636711586997 2315 82-83 1.0362876342145988 2315 84-85 0.9879812566218824 2315 86-87 1.0551978981108157 2315 88-89 1.1744644120548209 2315 90-91 1.2480424576302056 2315 92-93 1.29127472986913 2315 94-95 1.2803804576233695 2315 96-97 1.3909533089025992 2315 98-99 1.46215571995765 2315 100 1.5185269127507048 2316 1 0.1003003495842556 2316 2 0.04031673760887955 2316 3 0.11785588922525747 2316 4 0.11591596000528881 2316 5 0.11202803078344914 2316 6 0.14796385591635897 2316 7 0.20084729249845168 2316 8 0.18417435230595913 2316 9 0.3221372389099386 2316 10-11 0.18942847518149364 2316 12-13 0.3208279408116539 2316 14-15 0.4790674631482048 2316 16-17 0.5257880378080628 2316 18-19 0.4760060599425415 2316 20-21 0.4455475093687795 2316 22-23 0.3080650008893073 2316 24-25 0.4124151952014401 2316 26-27 0.4641882700401041 2316 28-29 0.5999349639376703 2316 30-31 0.5793528363735234 2316 32-33 0.7014607741580932 2316 34-35 0.6969109846230239 2316 36-37 0.7350530091214154 2316 38-39 0.8234971367696176 2316 40-41 0.786720945836656 2316 42-43 0.8401838750091386 2316 44-45 0.6678758648492078 2316 46-47 0.7164090050523697 2316 48-49 0.7532021229974859 2316 50-51 0.45555596123816855 2316 52-53 0.3003688489316403 2316 54-55 0.2932259935520065 2316 56-57 0.3485702615743449 2316 58-59 0.41058794094465867 2316 60-61 0.5179199194796666 2316 62-63 0.6044192532179977 2316 64-65 0.5530449427994881 2316 66-67 0.5307134669079474 2316 68-69 0.5323912266987207 2316 70-71 0.47987212420883196 2316 72-73 0.5613537958524404 2316 74-75 0.6547888228010237 2316 76-77 0.7262465429966696 2316 78-79 0.7629157894666676 2316 80-81 0.7948955865969118 2316 82-83 0.8755738935793396 2316 84-85 0.8903851669904341 2316 86-87 0.9345802313261693 2316 88-89 1.0393298416435819 2316 90-91 1.0474666587993227 2316 92-93 1.0652344848664583 2316 94-95 1.1167050197927146 2316 96-97 1.2255330056433174 2316 98-99 1.3398461646626707 2316 100 1.2643384059317988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 53.0 1 760.0 2 360.0 3 277.0 4 259.0 5 183.0 6 256.0 7 1273.0 8 4515.0 9 8136.0 10 12182.0 11 17531.0 12 23692.0 13 29397.0 14 34927.0 15 40813.0 16 49487.0 17 61983.0 18 75448.0 19 90248.0 20 106137.0 21 125922.0 22 137810.0 23 141193.0 24 142852.0 25 150862.0 26 167554.0 27 192801.0 28 228168.0 29 268405.0 30 322045.0 31 393794.0 32 494723.0 33 635753.0 34 870150.0 35 1173005.0 36 1703153.0 37 2446374.0 38 2335149.0 39 656459.0 40 730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55926518655872 19.304025477842984 12.458951898143523 23.677757437454776 2 15.743931837545805 20.580824174656232 40.56915362783877 23.106090359959193 3 17.761728192991917 27.761474802524745 30.230548039446425 24.24624896503691 4 11.989264260819537 17.17576736817107 39.548348377962725 31.286619993046667 5 12.645693658878685 38.28913213271896 35.118343186260816 13.94683102214154 6 29.183766752006928 38.25350967699212 19.195850385211003 13.366873185789952 7 26.301591452068223 32.95116103722802 23.913366667313706 16.833880843390048 8 25.76166519317125 35.131459514016726 21.071131579778644 18.035743713033376 9 27.28938372923656 15.153011700299302 21.00580988882075 36.55179468164339 10-11 24.536679640999072 26.05582273684207 29.336413928619788 20.07108369353907 12-13 25.213862913720092 24.266925879680574 28.81911977069025 21.70009143590909 14-15 22.865319985093656 25.462200268108703 26.581960244809462 25.09051950198818 16-17 21.870086354423044 27.834742518076087 27.544990917299316 22.750180210201545 18-19 21.567056190812618 27.492050013249187 29.359171242058334 21.58172255387986 20-21 22.675459146452237 26.887053159119723 28.790995983271078 21.646491711156955 22-23 22.571578815483846 26.698059894415966 28.59124152383041 22.139119766269776 24-25 22.08541023374929 26.736986136572018 29.001038245709072 22.17656538396962 26-27 22.047896466822735 26.85695767428065 28.748764858788718 22.346381000107897 28-29 21.85008108580486 27.37220587249475 28.748446518027997 22.02926652367239 30-31 22.692999562525205 26.83860870461085 28.715002349443942 21.753389383420004 32-33 22.56363258792677 27.04521863278235 28.371635513638218 22.019513265652666 34-35 22.639505247573112 27.086784507585804 28.73596306866401 21.537747176177078 36-37 22.42774872611894 26.873238457035413 28.654411932002148 22.044600884843497 38-39 22.318077848737776 27.055645423904394 28.740447099358 21.885829627999822 40-41 22.30235245063698 26.53462839190696 29.03842292793488 22.124596229521178 42-43 22.15606686191376 26.8172117121318 28.487519143168356 22.539202282786086 44-45 21.835675366396806 27.216481726860337 28.737618816419484 22.21022409032337 46-47 21.82429086435503 26.827797426968115 28.44046612451975 22.907445584157106 48-49 22.101701875810516 26.816904727140216 28.481720283785876 22.59967311326339 50-51 22.00809326930899 27.058731182905344 28.167889717733974 22.765285830051692 52-53 21.70886551149134 27.38146598103081 28.004787562748568 22.904880944729275 54-55 21.691574511288394 27.10964671356299 28.269220487367352 22.929558287781262 56-57 22.075245638137808 26.955167086906012 28.031125187334354 22.938462087621826 58-59 21.743523592412217 26.94825583211862 28.436270325690167 22.871950249779 60-61 22.101972425895823 27.231948510116744 27.94745483650956 22.718624227477875 62-63 22.030676239687534 26.928534728783347 27.97447542783952 23.066313603689597 64-65 21.689454222225873 26.666422398103194 28.091855024552988 23.552268355117942 66-67 21.90039846094839 26.519143780782557 27.872815161606507 23.70764259666255 68-69 21.618625201227122 26.746047927081907 27.99547306039428 23.639853811296692 70-71 21.852204604745708 26.38012096265846 28.151195446070492 23.616478986525337 72-73 22.06814710900624 26.62701310277778 27.82306264853628 23.4817771396797 74-75 22.160509583996518 26.7729140842712 27.662034927690136 23.404541404042146 76-77 21.98801231259204 26.554626816973993 27.732588725976647 23.72477214445732 78-79 21.781189091514904 26.579075689419707 27.89956774675637 23.74016747230902 80-81 21.92131507094477 26.731920898086692 27.70308712163149 23.643676909337046 82-83 22.436221845066687 26.37037383671803 27.923819654723875 23.269584663491408 84-85 22.51799973370361 26.609392654023928 27.369588952504138 23.503018659768323 86-87 22.005278163058346 26.8661588762004 27.718946167583486 23.409616793157767 88-89 22.317590688286618 26.195570269093338 27.67937330028063 23.807465742339417 90-91 22.72639525824453 26.03414117506156 27.694137566820377 23.545325999873537 92-93 22.896343008438368 26.155947970014566 27.422197829055506 23.525511192491557 94-95 22.452380063711256 25.980914016510958 27.660545395669416 23.906160524108362 96-97 22.61608781132864 25.824559056591468 27.625561968046515 23.933791164033373 98-99 22.49232002848525 26.090256887375475 27.36737878140406 24.05004430273522 100 22.922362017150203 25.773025399601668 27.10874711471934 24.19586546852879 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 884.0 1 719.0 2 1601.5 3 2506.5 4 1897.5 5 1610.0 6 2189.5 7 3768.0 8 6197.0 9 7674.0 10 7462.0 11 7355.5 12 8165.5 13 9297.0 14 10574.0 15 11880.0 16 13231.0 17 14590.0 18 15886.0 19 17248.0 20 18938.5 21 21093.0 22 24371.5 23 29031.5 24 35007.0 25 43128.0 26 55674.5 27 70413.5 28 86203.0 29 108560.5 30 138306.5 31 167288.5 32 203878.5 33 242421.0 34 277712.5 35 312163.0 36 346483.0 37 390617.0 38 411816.0 39 407170.0 40 400635.5 41 395580.0 42 400330.5 43 416219.5 44 439906.5 45 473075.0 46 497317.0 47 512640.5 48 529772.5 49 536901.0 50 533844.5 51 530264.5 52 517924.5 53 495392.5 54 468684.0 55 424738.5 56 378491.0 57 329004.0 58 277770.5 59 236624.5 60 191108.5 61 148116.5 62 116495.0 63 91142.0 64 67642.0 65 47935.0 66 35366.5 67 26940.5 68 20580.5 69 15881.5 70 12548.5 71 9607.0 72 7403.5 73 5933.5 74 4759.0 75 3665.5 76 2715.0 77 1939.0 78 1361.0 79 990.0 80 747.0 81 554.0 82 406.5 83 289.0 84 217.5 85 162.5 86 132.0 87 114.5 88 81.0 89 63.5 90 52.5 91 40.5 92 38.5 93 31.5 94 18.5 95 15.5 96 14.5 97 9.0 98 5.0 99 4.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22144846574152144 2 0.14811919433808865 3 0.023172627938049205 4 0.02221407537068407 5 0.02698401552733438 6 0.05488093826168318 7 0.06142343991195314 8 0.05419625785642237 9 0.04181875764131861 10-11 0.04412384833903 12-13 0.04230564148505962 14-15 0.021008277101419198 16-17 0.01922810804774109 18-19 0.0190303114862213 20-21 0.02211137330989495 22-23 0.025435877055439107 24-25 0.022362422791823912 26-27 0.019756833471803605 28-29 0.019536214230108456 30-31 0.01785874723721947 32-33 0.025865704198741728 34-35 0.02631074646216125 36-37 0.024998442352078033 38-39 0.023313367799130593 40-41 0.024538184968541595 42-43 0.030692701055830437 44-45 0.04011846796825426 46-47 0.044759079603910866 48-49 0.04112266589597011 50-51 0.028726146780720222 52-53 0.043929855557539435 54-55 0.04223336966450432 56-57 0.044717238023589366 58-59 0.047961862388519766 60-61 0.024998442352078033 62-63 0.02619282928125522 64-65 0.0269726041872467 66-67 0.03240059828895324 68-69 0.03663800924151181 70-71 0.040392340130358584 72-73 0.04391844421745176 74-75 0.025519560216082093 76-77 0.019026507706192075 78-79 0.01831520084072668 80-81 0.02157504032577398 82-83 0.01965793519104371 84-85 0.01193626173171346 86-87 0.01368980432518698 88-89 0.019597074710576083 90-91 0.020403476076772148 92-93 0.020578449958116578 94-95 0.02423388256620346 96-97 0.02495279699172731 98-99 0.03029710793279086 100 0.030300911712820084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3144819E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.23543761400235 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62295239576252 41.40218946410568 2 14.924519007554938 16.78573715136696 3 5.274479111534917 8.89837923069241 4 2.3114307601503525 5.1993728124608465 5 1.2030423335338254 3.382680604722262 6 0.6966767613573551 2.3506753530262046 7 0.45094553448926544 1.7751383630458824 8 0.29098004907562525 1.309071231736933 9 0.20123793637500942 1.0185033074928673 >10 0.939087528953608 9.14658691907173 >50 0.049376087856964734 1.9055971111793597 >100 0.031190414187313158 3.3868585617984586 >500 0.002741897616153128 1.055528829982893 >1k 0.0012601707599281106 1.3067396360326413 >5k 2.0002698075225347E-5 0.05949387077244686 >10k+ 6.000809422567605E-5 1.0174475525124957 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40763 0.3101069706627379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29407 0.22371551863894054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20281 0.1542889255454944 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 7.6075600584534485E-6 3 0.0 0.0 0.0 0.0 7.6075600584534485E-6 4 0.0 0.0 0.0 0.0 7.6075600584534485E-6 5 7.6075600584534485E-6 3.0430240233813794E-5 0.0 0.0 7.6075600584534485E-6 6 7.6075600584534485E-6 3.0430240233813794E-5 0.0 0.0 1.5215120116906897E-5 7 7.6075600584534485E-6 3.0430240233813794E-5 0.0 0.0 1.5215120116906897E-5 8 7.6075600584534485E-6 3.0430240233813794E-5 0.0 0.0 1.5215120116906897E-5 9 7.6075600584534485E-6 3.0430240233813794E-5 0.0 0.0 3.0430240233813794E-5 10-11 2.2822680175360346E-5 3.0430240233813794E-5 0.0 7.6075600584534485E-6 3.423402026304052E-5 12-13 2.2822680175360346E-5 4.944914037994742E-5 0.0 4.944914037994742E-5 6.086048046762759E-5 14-15 2.2822680175360346E-5 5.325292040917414E-5 0.0 6.466426049685431E-5 9.50945007306681E-5 16-17 3.0430240233813794E-5 5.325292040917414E-5 0.0 6.846804052608104E-5 9.889828075989483E-5 18-19 3.0430240233813794E-5 5.325292040917414E-5 0.0 1.1791718090602846E-4 1.0650584081834828E-4 20-21 3.0430240233813794E-5 6.086048046762759E-5 0.0 2.700683820750974E-4 1.10309620847575E-4 22-23 3.0430240233813794E-5 6.086048046762759E-5 0.0 9.50945007306681E-4 1.445436411106155E-4 24-25 3.0430240233813794E-5 9.889828075989483E-5 0.0 0.0024458305587927837 1.5975876122752242E-4 26-27 3.0430240233813794E-5 9.889828075989483E-5 0.0 0.004800370396884126 1.6736632128597586E-4 28-29 3.0430240233813794E-5 1.0650584081834828E-4 0.0 0.01340071704296575 1.711701013152026E-4 30-31 3.0430240233813794E-5 1.0650584081834828E-4 0.0 0.04877587131477429 1.7877766137365604E-4 32-33 3.0430240233813794E-5 1.0650584081834828E-4 0.0 0.1267952034942436 2.1301168163669656E-4 34-35 3.0430240233813794E-5 1.10309620847575E-4 0.0 0.23130405979724789 2.1301168163669656E-4 36-37 3.0430240233813794E-5 1.1791718090602846E-4 0.0 0.3988415511845389 2.1301168163669656E-4 38-39 3.0430240233813794E-5 1.2172096093525518E-4 0.0 0.688902601093252 2.168154616659233E-4 40-41 3.0430240233813794E-5 1.2172096093525518E-4 0.0 1.0381961136170836 2.2822680175360344E-4 42-43 3.0430240233813794E-5 1.2172096093525518E-4 0.0 1.365503777571985 2.358343618120569E-4 44-45 3.423402026304052E-5 1.2172096093525518E-4 0.0 1.7190727388486673 2.5865704198741726E-4 46-47 4.564536035072069E-5 1.2172096093525518E-4 0.0 2.12719170952449 2.6246082201664394E-4 48-49 5.325292040917414E-5 1.2172096093525518E-4 0.0 2.5768974072598487 2.814797221627776E-4 50-51 5.325292040917414E-5 1.2172096093525518E-4 0.0 3.0657896468563015 2.8528350219200434E-4 52-53 5.325292040917414E-5 1.2932852099370863E-4 0.0 3.548432275864734 3.3853642260117846E-4 54-55 5.705670043840086E-5 1.445436411106155E-4 0.0 4.041866228816083 3.803780029226724E-4 56-57 6.466426049685431E-5 1.5215120116906898E-4 0.0 4.567856735037584 3.9939690306880604E-4 58-59 6.846804052608104E-5 1.5975876122752242E-4 0.0 5.176480558614006 4.2221958324416644E-4 60-61 6.846804052608104E-5 1.6736632128597586E-4 0.0 5.79921260231883 4.336309233318466E-4 62-63 6.846804052608104E-5 1.6736632128597586E-4 0.0 6.433896122875485 4.678649435948871E-4 64-65 7.607560058453449E-5 1.749738813444293E-4 0.0 7.032124215632029 4.716687236241138E-4 66-67 7.987938061376121E-5 1.749738813444293E-4 0.0 7.664681423152347 4.716687236241138E-4 68-69 8.368316064298793E-5 1.749738813444293E-4 0.0 8.360274873317008 4.7927628368256723E-4 70-71 8.368316064298793E-5 1.7877766137365604E-4 0.0 9.094229445076422 4.944914037994741E-4 72-73 8.368316064298793E-5 1.8258144140288277E-4 0.0 9.7938206680518 5.09706523916381E-4 74-75 8.368316064298793E-5 1.8258144140288277E-4 0.0 10.455944657739296 5.135103039456078E-4 76-77 8.368316064298793E-5 1.8258144140288277E-4 0.0 11.152051618207905 5.287254240625147E-4 78-79 8.368316064298793E-5 1.8258144140288277E-4 0.0 11.90305853583834 5.401367641501948E-4 80-81 8.368316064298793E-5 1.8258144140288277E-4 0.0 12.663955281544766 5.439405441794215E-4 82-83 8.368316064298793E-5 1.901890014613362E-4 0.0 13.434338654644083 5.857821245009155E-4 84-85 8.368316064298793E-5 1.901890014613362E-4 0.0 14.161701275612849 5.93389684559369E-4 86-87 8.368316064298793E-5 1.9779656151978965E-4 0.0 14.887120164986676 5.93389684559369E-4 88 8.368316064298793E-5 1.9779656151978965E-4 0.0 15.43774775445748 5.93389684559369E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20940 0.0 44.87483 1 GTATCAA 37870 0.0 40.791336 1 CAACGCA 46310 0.0 32.8678 5 ATCAACG 46295 0.0 32.807373 3 TACCTGG 9645 0.0 32.697296 2 TATCAAC 48470 0.0 31.609468 2 AACGCAG 49550 0.0 31.193565 6 TCAACGC 49100 0.0 31.124502 4 GTACATG 46255 0.0 28.96493 1 TACATGG 46055 0.0 28.494118 2 ACGCAGA 54230 0.0 28.085505 7 ACATGGG 46430 0.0 27.487783 3 CGCAGAG 55630 0.0 27.29243 8 CATGGGG 34575 0.0 25.750443 4 GTACTAG 5805 0.0 25.653059 1 ACCTGGG 12265 0.0 25.218945 3 GCAGAGT 60425 0.0 24.324934 9 ATGGGGG 26435 0.0 24.166222 5 TGGGGGG 28150 0.0 21.959589 6 TAGGCAT 7520 0.0 21.065931 5 >>END_MODULE