FastQCFastQC Report
Mon 6 Jun 2016
ERR1211370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13144819
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367200.2793496053464106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283100.21537002525481713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201210.15307171593614183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212600.048.5213051
GTATCAA373700.039.3804241
TACCTGG97450.031.6829872
CAACGCA469800.030.5655175
ATCAACG470700.030.5015013
TATCAAC490600.029.6849732
AACGCAG504350.028.9359466
TCAACGC497800.028.8416714
GTACATG462550.026.9266571
TACATGG461850.026.5571942
ACGCAGA553100.025.969297
CGCAGAG562000.025.4827888
ACATGGG464950.025.41053
ACCTGGG118750.025.169683
CATGGGG345100.023.4096574
GCAGAGT622000.022.5561189
ATGGGGG253400.022.5485365
GTACTAG58350.022.4813561
TGGGGGG247350.022.2630166
AGAGTAC584300.020.55295410-11
Produced by FastQC (version 0.11.5)