FastQCFastQC Report
Mon 6 Jun 2016
ERR1211369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601299
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128100.35570498311859133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120630.33496246770956817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86750.2408853027754707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61800.053.8133281
TACCTGG28950.042.7545132
GTATCAA107400.038.0266841
ACCTGGG33500.036.066023
GTACATG151550.033.600321
TACATGG153750.032.2932782
ACATGGG152750.031.6697083
CATGGGG110700.031.1290784
GTACTAG20100.030.4663851
ATGGGGG87800.029.8778695
CAACGCA135600.029.1225595
ATCAACG136650.028.9331873
GTACACG5250.028.7120571
TGGGGGG93550.028.6444856
TATCAAC143200.028.0337242
AACGCAG144750.027.8662576
GCATCGT20100.027.5968598
TCAACGC145750.027.1267244
CCTGGGG35100.026.787544
CTGGGGG26750.025.6589575
Produced by FastQC (version 0.11.5)