FastQCFastQC Report
Mon 6 Jun 2016
ERR1211369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601299
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113160.3142199523005449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110230.30608399913475665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85170.236497996972759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.051.8760761
TACCTGG27200.043.8845522
GTATCAA108200.035.9380381
ACCTGGG33100.035.2108543
GTACATG146300.030.5641481
TACATGG147550.030.0344262
ACATGGG147600.029.2605193
ATGGGGG84350.028.2425165
TGGGGGG82900.028.1110786
CATGGGG107650.027.8962444
TCTAACG7350.027.493372
CTGGGGG24200.027.1827375
ATCAACG140350.027.088753
CCTGGGG32800.027.0798874
CAACGCA140000.027.051265
TATCAAC146500.026.239922
CTAACGC7450.025.8630923
AACGCAG149800.025.6561726
TAACGCC7550.025.520894
TCAACGC151050.025.2324264
Produced by FastQC (version 0.11.5)