FastQCFastQC Report
Mon 6 Jun 2016
ERR1211368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908004
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287700.2068593020249347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220680.15867122270025233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144160.10365254424718312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188700.046.8890761
GTATCAA330600.040.2590561
CAACGCA388850.033.1002465
ATCAACG394550.032.551033
TATCAAC421150.031.1674422
TACCTGG93650.031.0075072
AACGCAG429900.030.4539646
TCAACGC425950.030.3496654
GTACATG523150.029.899221
TACATGG519450.029.2377992
ACATGGG518650.028.1250613
ACGCAGA471200.027.1865167
CATGGGG394350.026.7140564
CGCAGAG484250.026.3938188
GTACTAG64900.025.916211
ATGGGGG304550.025.761885
ACCTGGG118100.024.0819443
TGGGGGG309600.023.8084536
GCAGAGT536850.023.2724869
CTGTGCG85900.021.7239179
Produced by FastQC (version 0.11.5)