FastQCFastQC Report
Mon 6 Jun 2016
ERR1211368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908004
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258840.1861086608833302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214820.15445782155368953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149020.10714693495917889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187300.047.2219161
GTATCAA329850.039.458651
CAACGCA406900.031.1448295
ATCAACG410350.030.8769683
TACCTGG93800.030.61292
TATCAAC436200.029.5748652
AACGCAG445600.029.0289866
TCAACGC445900.028.563334
GTACATG519350.026.953261
TACATGG516050.026.6105922
GTACTAG68900.026.0014971
ACGCAGA492800.025.7242137
ACATGGG514300.025.4859713
CGCAGAG506200.025.006038
ACCTGGG112650.024.864763
CATGGGG387150.023.7446984
TGGGGGG277750.023.1928566
ATGGGGG294650.023.171385
GCAGAGT565700.021.9688459
TAGGCAT89350.021.0357555
Produced by FastQC (version 0.11.5)