##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211368_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13908004 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.913643179855285 31.0 31.0 34.0 30.0 34.0 2 32.061255878269804 33.0 31.0 34.0 30.0 34.0 3 32.135107812738624 33.0 31.0 34.0 30.0 34.0 4 35.8169271449735 37.0 35.0 37.0 35.0 37.0 5 35.663065814476326 37.0 35.0 37.0 33.0 37.0 6 35.69573685771157 37.0 35.0 37.0 33.0 37.0 7 35.618499750215776 37.0 35.0 37.0 33.0 37.0 8 35.60088852433462 37.0 35.0 37.0 33.0 37.0 9 37.1579037509624 39.0 37.0 39.0 33.0 39.0 10-11 37.12668194515906 39.0 37.0 39.0 33.0 39.0 12-13 37.03411898644838 39.0 37.0 39.0 33.0 39.0 14-15 38.197685555741856 40.0 38.0 41.0 33.0 41.0 16-17 38.18414410867297 40.0 38.0 41.0 33.0 41.0 18-19 38.17731530707066 40.0 37.5 41.0 33.0 41.0 20-21 38.133607345813246 40.0 37.0 41.0 33.0 41.0 22-23 38.02495243026965 40.0 37.0 41.0 33.0 41.0 24-25 37.898268651633984 40.0 37.0 41.0 33.0 41.0 26-27 37.76726056449222 40.0 37.0 41.0 32.5 41.0 28-29 37.6903255492305 40.0 37.0 41.0 32.0 41.0 30-31 37.75094848261476 40.0 37.0 41.0 33.0 41.0 32-33 37.60321427143679 40.0 36.5 41.0 32.0 41.0 34-35 37.49317695048117 40.0 36.0 41.0 32.0 41.0 36-37 37.348461432711694 39.5 36.0 41.0 31.5 41.0 38-39 37.22896599684613 39.0 36.0 41.0 31.0 41.0 40-41 37.1690565375161 39.0 36.0 41.0 31.0 41.0 42-43 37.0416741683422 39.0 36.0 41.0 31.0 41.0 44-45 36.89216479230233 39.0 35.5 41.0 30.5 41.0 46-47 36.68367366014563 39.0 35.0 40.5 30.0 41.0 48-49 36.4779177874841 39.0 35.0 40.0 30.0 41.0 50-51 36.295179308260195 39.0 35.0 40.0 29.5 41.0 52-53 36.08236113535774 38.0 35.0 40.0 29.0 41.0 54-55 35.87645225727574 38.0 34.5 40.0 28.0 41.0 56-57 35.63632480980017 38.0 34.0 40.0 28.0 41.0 58-59 35.36303397669428 38.0 34.0 40.0 28.0 41.0 60-61 35.0986465778986 37.0 34.0 40.0 27.0 41.0 62-63 34.799982981023014 37.0 33.5 40.0 26.5 41.0 64-65 34.44899160224573 36.0 33.0 39.0 26.0 41.0 66-67 34.106107102068705 36.0 33.0 39.0 26.0 40.0 68-69 33.745781134374134 35.0 32.5 38.5 26.0 40.0 70-71 33.388572292616544 35.0 32.0 38.0 25.0 40.0 72-73 33.039142676404175 35.0 32.0 37.0 25.0 39.5 74-75 32.67388293100865 35.0 31.0 37.0 24.5 39.0 76-77 31.70565255086208 34.0 30.5 35.5 23.5 38.0 78-79 32.2178591550592 34.0 31.0 36.0 24.5 38.0 80-81 32.113541130704306 34.0 31.0 35.5 24.5 37.0 82-83 31.838636910084297 34.0 31.0 35.0 24.0 37.0 84-85 31.556899394046766 34.0 31.0 35.0 23.5 36.5 86-87 31.291189195804087 34.0 31.0 35.0 23.0 36.0 88-89 31.046846010398042 34.0 30.0 35.0 22.5 36.0 90-91 30.814802828644574 34.0 30.0 35.0 20.0 35.0 92-93 30.60900755421123 34.0 30.0 35.0 20.0 35.0 94-95 30.41700843629323 34.0 29.0 35.0 20.0 35.0 96-97 30.219738540483597 34.0 29.0 35.0 19.0 35.0 98-99 29.99720168328971 33.5 29.0 35.0 19.0 35.0 100 28.14745178387927 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34324173540408864 1101 2 -0.3127596848035381 1101 3 -0.4023832802683849 1101 4 -0.2207766912014577 1101 5 -0.2565588238821377 1101 6 -0.3091958785516553 1101 7 -0.34943879400124445 1101 8 -0.4156649794817042 1101 9 -0.5120698938308692 1101 10-11 -0.498929628964099 1101 12-13 -0.5398586860011605 1101 14-15 -0.7112800868755826 1101 16-17 -0.7375562693655482 1101 18-19 -0.7468371080742386 1101 20-21 -0.7484034807104507 1101 22-23 -0.8385639920006867 1101 24-25 -0.8720507664690089 1101 26-27 -0.8796188000412357 1101 28-29 -0.8436296005210338 1101 30-31 -0.8032369516167961 1101 32-33 -0.7101139761697439 1101 34-35 -0.7799417296345155 1101 36-37 -0.853098866442842 1101 38-39 -0.9095009220542778 1101 40-41 -0.8383933843572606 1101 42-43 -0.8387253936413472 1101 44-45 -0.8930802722897155 1101 46-47 -0.949321012696899 1101 48-49 -0.9168702238163817 1101 50-51 -0.9862643761522634 1101 52-53 -0.9918116829431796 1101 54-55 -1.01010059481208 1101 56-57 -0.9908026323126435 1101 58-59 -0.9999572538444355 1101 60-61 -0.956189439490899 1101 62-63 -1.1208419380757846 1101 64-65 -1.063524636355524 1101 66-67 -1.1441915453972413 1101 68-69 -1.1112547886068924 1101 70-71 -1.0940473639718604 1101 72-73 -1.1649433460642342 1101 74-75 -1.2195405473390366 1101 76-77 -1.0183868551717126 1101 78-79 -0.9782526408462466 1101 80-81 -0.9379131244310415 1101 82-83 -1.0214384826402458 1101 84-85 -1.0262934583672134 1101 86-87 -1.2013490508133131 1101 88-89 -1.225927582122086 1101 90-91 -1.3095033974564814 1101 92-93 -1.3390502699596993 1101 94-95 -1.4766351504999697 1101 96-97 -1.5259169826352341 1101 98-99 -1.679869867251277 1101 100 -1.666890192990504 1102 1 -0.40333004170421205 1102 2 -0.35363704180510425 1102 3 -0.36689386115916633 1102 4 -0.2070709552372847 1102 5 -0.30123900744899856 1102 6 -0.4015365070886787 1102 7 -0.34487250042027995 1102 8 -0.4283009121246337 1102 9 -0.5647317036039681 1102 10-11 -0.5384019009344243 1102 12-13 -0.5407899933894669 1102 14-15 -0.706676580697966 1102 16-17 -0.7686224848481586 1102 18-19 -0.7585294143939265 1102 20-21 -0.8029425570166566 1102 22-23 -0.7963714863782272 1102 24-25 -0.8665458805481805 1102 26-27 -0.9273385170961745 1102 28-29 -0.9015638530556416 1102 30-31 -0.8701872423201209 1102 32-33 -0.9027952559990382 1102 34-35 -0.935585998574382 1102 36-37 -0.8815734604096193 1102 38-39 -0.9398615204033618 1102 40-41 -0.9432477793552394 1102 42-43 -0.9935193900472896 1102 44-45 -0.9652845068888141 1102 46-47 -1.110455955763065 1102 48-49 -1.0803835807852948 1102 50-51 -1.153283206790256 1102 52-53 -1.17613692893665 1102 54-55 -1.2127729606027557 1102 56-57 -1.158487585824325 1102 58-59 -1.1722948664933668 1102 60-61 -1.2706514025385616 1102 62-63 -1.188088312947805 1102 64-65 -1.1753969669246942 1102 66-67 -1.2557527103109223 1102 68-69 -1.3229287045165705 1102 70-71 -1.2629246348636656 1102 72-73 -1.357827652991439 1102 74-75 -1.3183390603330878 1102 76-77 -1.110324468319952 1102 78-79 -1.0838681291116359 1102 80-81 -0.999120161915247 1102 82-83 -1.0133643621052784 1102 84-85 -1.0540968238552217 1102 86-87 -1.2112469876188534 1102 88-89 -1.2127280275109236 1102 90-91 -1.2950084311226746 1102 92-93 -1.3944227979285415 1102 94-95 -1.5373330496775068 1102 96-97 -1.5662691376026885 1102 98-99 -1.5463403784119762 1102 100 -1.5227309059695315 1103 1 -0.4197988639838961 1103 2 -0.3767053991258962 1103 3 -0.3923614993881941 1103 4 -0.2349209823429419 1103 5 -0.24475241717482987 1103 6 -0.35728175993483546 1103 7 -0.3574661762415019 1103 8 -0.399373758903252 1103 9 -0.5477096975737297 1103 10-11 -0.5360459100633719 1103 12-13 -0.5438031751435375 1103 14-15 -0.769114257261343 1103 16-17 -0.8745963747070391 1103 18-19 -0.7870158336480557 1103 20-21 -0.7469102715391145 1103 22-23 -0.8458966146918883 1103 24-25 -0.8277701244145774 1103 26-27 -0.9004132816116623 1103 28-29 -0.882840829537578 1103 30-31 -0.8401991517407907 1103 32-33 -0.8118831965952893 1103 34-35 -0.8039680396097566 1103 36-37 -0.818071962729114 1103 38-39 -0.8780885284589317 1103 40-41 -0.9395168881547349 1103 42-43 -0.9325341051671145 1103 44-45 -0.8912698966543715 1103 46-47 -0.9256655053521143 1103 48-49 -0.9880136852148595 1103 50-51 -0.9678522421713325 1103 52-53 -1.1008067491100704 1103 54-55 -0.9658577198170448 1103 56-57 -1.083278315256642 1103 58-59 -1.1286652725665647 1103 60-61 -1.0789572932186786 1103 62-63 -1.1820793966874632 1103 64-65 -1.0778330475612208 1103 66-67 -1.1923173809390946 1103 68-69 -1.1336476488310865 1103 70-71 -1.065695982621186 1103 72-73 -1.1196054285438706 1103 74-75 -1.2352255774640852 1103 76-77 -1.0402517314337558 1103 78-79 -0.9280104076384745 1103 80-81 -0.9255901039818397 1103 82-83 -0.9298087589110509 1103 84-85 -0.9685430178251018 1103 86-87 -1.0099424765870104 1103 88-89 -1.0231133508362866 1103 90-91 -1.1583566635643834 1103 92-93 -1.145562284915929 1103 94-95 -1.2172647629331017 1103 96-97 -1.2692565593450524 1103 98-99 -1.3688928638553683 1103 100 -1.4399944485350389 1104 1 -0.48654514008009997 1104 2 -0.40539406638790965 1104 3 -0.45652930553029947 1104 4 -0.31076524507807335 1104 5 -0.35162222638027174 1104 6 -0.41145756091842145 1104 7 -0.37714297437469213 1104 8 -0.4273230045336831 1104 9 -0.5665791440497046 1104 10-11 -0.5365546822154315 1104 12-13 -0.6315853730780816 1104 14-15 -0.8132224452086447 1104 16-17 -0.8078811337448073 1104 18-19 -0.8790182506411455 1104 20-21 -0.7883581033427731 1104 22-23 -0.8187359806119545 1104 24-25 -0.9409416157752801 1104 26-27 -0.9255742374158302 1104 28-29 -1.0058321073863752 1104 30-31 -0.9686006928387485 1104 32-33 -0.856757897743563 1104 34-35 -0.9567909579225073 1104 36-37 -0.9163484471154462 1104 38-39 -1.0560214795812897 1104 40-41 -1.1732935391877248 1104 42-43 -1.0012813152014388 1104 44-45 -1.051916510319053 1104 46-47 -1.0385631282505088 1104 48-49 -1.0738096476547767 1104 50-51 -1.1230741230401478 1104 52-53 -1.0047483530409806 1104 54-55 -1.0031410721990568 1104 56-57 -1.1268746474852023 1104 58-59 -1.2249039902522512 1104 60-61 -1.1406164821652993 1104 62-63 -1.1496066077900124 1104 64-65 -1.1597147474641076 1104 66-67 -1.187312062260716 1104 68-69 -1.152011645428324 1104 70-71 -1.1136550123465625 1104 72-73 -1.06249943807763 1104 74-75 -1.0920794897878707 1104 76-77 -0.9958063294740995 1104 78-79 -0.946199203367172 1104 80-81 -0.9813225535154757 1104 82-83 -1.0486320388676411 1104 84-85 -0.9610674777518007 1104 86-87 -0.9294405893886122 1104 88-89 -1.0097851881287134 1104 90-91 -0.9915810049689853 1104 92-93 -1.208451277424576 1104 94-95 -1.2783669315555173 1104 96-97 -1.3672987776826737 1104 98-99 -1.235717605115184 1104 100 -1.282283524415739 1105 1 -0.3578228429610668 1105 2 -0.3499435112496023 1105 3 -0.3829659656975757 1105 4 -0.2159882209878532 1105 5 -0.2741781229931277 1105 6 -0.307144256370826 1105 7 -0.37439341836361706 1105 8 -0.3191230660244244 1105 9 -0.4849650261330751 1105 10-11 -0.5344320220428997 1105 12-13 -0.5694713591859681 1105 14-15 -0.7524981135980724 1105 16-17 -0.7477942524891859 1105 18-19 -0.7195724506484353 1105 20-21 -0.733633334364356 1105 22-23 -0.7812035383335711 1105 24-25 -0.7218961622536355 1105 26-27 -0.7659787131043316 1105 28-29 -0.754909130517909 1105 30-31 -0.7905852483695526 1105 32-33 -0.7005374300151033 1105 34-35 -0.7651059551277584 1105 36-37 -0.9013318007173226 1105 38-39 -0.8171587271499945 1105 40-41 -0.8121088024034435 1105 42-43 -0.8065890082248117 1105 44-45 -0.8408034333323613 1105 46-47 -0.9668057455848853 1105 48-49 -0.9085721379540601 1105 50-51 -0.923929385436189 1105 52-53 -0.9159340456150034 1105 54-55 -0.9182271038530772 1105 56-57 -0.9118838546692132 1105 58-59 -0.9205606675411815 1105 60-61 -1.015484257350458 1105 62-63 -0.9710575027946788 1105 64-65 -0.978925587010977 1105 66-67 -0.9383745432811494 1105 68-69 -1.0205030395624064 1105 70-71 -1.0487892659561382 1105 72-73 -1.1050495353968302 1105 74-75 -1.1147005447062242 1105 76-77 -1.039557089034389 1105 78-79 -0.9588277224472534 1105 80-81 -0.8455869522339974 1105 82-83 -0.8568253167472513 1105 84-85 -0.6884219847717539 1105 86-87 -0.7954592368240192 1105 88-89 -0.8813914409212948 1105 90-91 -0.9434519278690772 1105 92-93 -1.0324675918328339 1105 94-95 -0.9594700302001513 1105 96-97 -1.0386075101346286 1105 98-99 -1.195096849606891 1105 100 -1.1401925160902238 1106 1 -0.4377405812632631 1106 2 -0.3963086568292553 1106 3 -0.4456229285618143 1106 4 -0.2831249227841113 1106 5 -0.3783963692011767 1106 6 -0.37575897827672833 1106 7 -0.43562834411044093 1106 8 -0.4178177261140448 1106 9 -0.5125409035078263 1106 10-11 -0.543353298269146 1106 12-13 -0.6151336543202603 1106 14-15 -0.7614954632337927 1106 16-17 -0.8200200847382533 1106 18-19 -0.804315432793949 1106 20-21 -0.7754883022234651 1106 22-23 -0.7792401657874279 1106 24-25 -0.8971270182693942 1106 26-27 -0.8775672301946642 1106 28-29 -0.9098911487305443 1106 30-31 -0.8671995974324886 1106 32-33 -0.9404662216596051 1106 34-35 -0.8361281718934066 1106 36-37 -0.9235278067863604 1106 38-39 -0.9469694160436433 1106 40-41 -0.9276792841738555 1106 42-43 -0.9044245524360477 1106 44-45 -0.979089274950887 1106 46-47 -1.0224839415225944 1106 48-49 -1.0537632052047812 1106 50-51 -1.0847453058179468 1106 52-53 -1.1044009931558634 1106 54-55 -1.140339528131527 1106 56-57 -1.2319525038919679 1106 58-59 -1.1730307364323025 1106 60-61 -1.1948229109739472 1106 62-63 -1.0869007084448938 1106 64-65 -1.0951455720218632 1106 66-67 -1.07582453871958 1106 68-69 -1.1107246015218166 1106 70-71 -1.1178673492241487 1106 72-73 -1.175022221854121 1106 74-75 -1.1427231738987587 1106 76-77 -1.1459069991977096 1106 78-79 -0.9779785113487733 1106 80-81 -0.8338543904648432 1106 82-83 -0.8854869311635447 1106 84-85 -0.9354737262512138 1106 86-87 -1.0091725943534016 1106 88-89 -1.0647917360289476 1106 90-91 -0.9884390027721892 1106 92-93 -1.0435227405465355 1106 94-95 -1.0578064745374753 1106 96-97 -1.0190923039978408 1106 98-99 -1.150963669272791 1106 100 -1.2485328635290927 1107 1 -0.4352122722340397 1107 2 -0.4115292252703462 1107 3 -0.45635126975788154 1107 4 -0.2847453520643768 1107 5 -0.3074642490874382 1107 6 -0.4035064484476152 1107 7 -0.4232758102114431 1107 8 -0.4710784192605004 1107 9 -0.5802384782945182 1107 10-11 -0.5730717108942329 1107 12-13 -0.6371941208767993 1107 14-15 -0.780140862762174 1107 16-17 -0.7669480162974125 1107 18-19 -0.7961192621194186 1107 20-21 -0.814734456790049 1107 22-23 -0.8638133630099034 1107 24-25 -0.8600465050513222 1107 26-27 -0.8274632763697767 1107 28-29 -0.9039893480737717 1107 30-31 -0.9270403041321629 1107 32-33 -0.8939174396110587 1107 34-35 -0.9435207476289094 1107 36-37 -0.9504397603484236 1107 38-39 -0.9554479966522393 1107 40-41 -0.9243368806241179 1107 42-43 -0.9111066135854173 1107 44-45 -0.9571908194497425 1107 46-47 -1.052495214628422 1107 48-49 -1.0430071225575617 1107 50-51 -1.011769904627279 1107 52-53 -1.0265880308494246 1107 54-55 -1.0721849304058608 1107 56-57 -1.0094432706347902 1107 58-59 -1.019884012473355 1107 60-61 -1.0643722674654512 1107 62-63 -1.091738104068149 1107 64-65 -1.0755176985199313 1107 66-67 -0.9841550402918742 1107 68-69 -0.9958785978353788 1107 70-71 -1.0107722861482031 1107 72-73 -1.0652969186105175 1107 74-75 -1.046088102820331 1107 76-77 -1.062801910707016 1107 78-79 -0.8717314135973595 1107 80-81 -0.810871296936309 1107 82-83 -0.8423231332791978 1107 84-85 -0.8448135054572141 1107 86-87 -0.8448096817437509 1107 88-89 -0.850581738515011 1107 90-91 -0.8790256468141848 1107 92-93 -1.004808429929561 1107 94-95 -0.987462171623875 1107 96-97 -1.01927400253469 1107 98-99 -1.0668010038476865 1107 100 -1.0601565193007119 1108 1 -0.4899893461874747 1108 2 -0.48073606878655895 1108 3 -0.5062223750345929 1108 4 -0.3503539422484465 1108 5 -0.3624552275149284 1108 6 -0.4253630693857531 1108 7 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68-69 1.117087474807569 2115 70-71 1.1685009167349847 2115 72-73 1.1602718030703514 2115 74-75 0.8322504239364363 2115 76-77 0.8048140029917903 2115 78-79 0.6684227733864248 2115 80-81 0.8322893587824645 2115 82-83 0.9483278470157366 2115 84-85 0.9963558676734685 2115 86-87 1.053376733643919 2115 88-89 1.0513362621954059 2115 90-91 1.1321310793306978 2115 92-93 1.167868406343466 2115 94-95 1.1686133484309558 2115 96-97 1.2507359108320308 2115 98-99 1.3249686376752052 2115 100 1.2256821565329616 2116 1 0.2025001243977549 2116 2 0.32868279858133675 2116 3 0.3973709467985671 2116 4 0.2690627209766987 2116 5 0.38644537279647295 2116 6 0.30369660549801125 2116 7 0.4276773362868198 2116 8 0.48088555615264994 2116 9 0.5779582378372226 2116 10-11 0.6245939442316484 2116 12-13 0.5178988986581032 2116 14-15 0.7086528788488451 2116 16-17 0.7997009801922275 2116 18-19 0.6897336802853076 2116 20-21 0.8119166964376348 2116 22-23 0.8709454978410704 2116 24-25 0.9338482817639431 2116 26-27 0.9506797900705095 2116 28-29 0.9356531435218827 2116 30-31 0.7005827516582386 2116 32-33 0.7491806731832042 2116 34-35 0.7890624601849652 2116 36-37 0.8404055576228302 2116 38-39 0.769785980207871 2116 40-41 0.865774654385973 2116 42-43 0.9566079810881121 2116 44-45 1.0148818973591531 2116 46-47 0.9021516355537926 2116 48-49 0.7736378434909881 2116 50-51 0.9469472056886374 2116 52-53 0.9565585012943529 2116 54-55 0.9678730906249129 2116 56-57 0.9960793853548964 2116 58-59 1.005179454363791 2116 60-61 0.9902153890036942 2116 62-63 1.0203636992968015 2116 64-65 0.8776095373755908 2116 66-67 0.8555173013119699 2116 68-69 0.8237312073113046 2116 70-71 0.8129085357321131 2116 72-73 0.9633439477513974 2116 74-75 0.8992635652036469 2116 76-77 0.8070321106849434 2116 78-79 0.8293687293210326 2116 80-81 0.7835476099608591 2116 82-83 0.7466132602505162 2116 84-85 0.7361230953904006 2116 86-87 0.7663751748602898 2116 88-89 0.7557875451727263 2116 90-91 0.5648888382465742 2116 92-93 0.761212459194688 2116 94-95 0.8630736219858726 2116 96-97 0.999410527541766 2116 98-99 1.0721107178627207 2116 100 0.6387685796673175 2201 1 0.7372799931987863 2201 2 0.6799902689232873 2201 3 0.6420366403629316 2201 4 0.4664288629765707 2201 5 0.5394492270684097 2201 6 0.5456512802753508 2201 7 0.5961334742225262 2201 8 0.5991616445321526 2201 9 0.7471617502696191 2201 10-11 0.7098729842189186 2201 12-13 0.7298452471605827 2201 14-15 0.8887782347876012 2201 16-17 0.8641364557927389 2201 18-19 0.8970215393055341 2201 20-21 0.8898421431276233 2201 22-23 0.8851342184206104 2201 24-25 0.8301443864303693 2201 26-27 0.904483366474679 2201 28-29 0.9241771324297758 2201 30-31 1.0667089407803232 2201 32-33 0.8853161548269952 2201 34-35 1.037697721420102 2201 36-37 1.0565144877046961 2201 38-39 1.1122791924703819 2201 40-41 1.091662236582188 2201 42-43 0.1605921497710412 2201 44-45 0.8401935752753857 2201 46-47 1.0465504887308725 2201 48-49 1.1330416534310643 2201 50-51 1.1240884175252503 2201 52-53 1.1464516934969708 2201 54-55 1.129225318515644 2201 56-57 1.1364241551415617 2201 58-59 1.161413607907619 2201 60-61 1.178086295088491 2201 62-63 1.2240381532831819 2201 64-65 1.221901990685005 2201 66-67 1.210215872745799 2201 68-69 1.2323372205103809 2201 70-71 1.2886021158478016 2201 72-73 1.2408613473745191 2201 74-75 1.1912695068034864 2201 76-77 1.2491940413987166 2201 78-79 1.0191351074137032 2201 80-81 0.9381245297110148 2201 82-83 0.9412720312073652 2201 84-85 0.902217977218772 2201 86-87 0.8710448963060493 2201 88-89 0.8409159121022078 2201 90-91 0.2119929664613842 2201 92-93 0.5622245996896353 2201 94-95 0.7424863809035109 2201 96-97 0.6780560497070951 2201 98-99 0.6467404683718527 2201 100 0.842267239412827 2202 1 0.6925508364701507 2202 2 0.6627225461253978 2202 3 0.651633308065307 2202 4 0.43209983322245193 2202 5 0.4631132190366287 2202 6 0.5132692448487362 2202 7 0.5528361913409583 2202 8 0.5525971911976839 2202 9 0.7729396397612405 2202 10-11 0.7835836604168023 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1.0995621714862551 2202 80-81 1.0500755909191888 2202 82-83 1.0055957208525577 2202 84-85 0.9354798559376896 2202 86-87 0.956844565160285 2202 88-89 0.9698830980786255 2202 90-91 0.9992595761520562 2202 92-93 0.925849530350213 2202 94-95 0.8861764416396944 2202 96-97 0.6615985223908574 2202 98-99 0.6048484168426782 2202 100 0.8516192750759899 2203 1 0.7291250568813474 2203 2 0.7152574099868723 2203 3 0.6787626208078095 2203 4 0.44086692690655127 2203 5 0.5646784620933758 2203 6 0.5233067567191654 2203 7 0.5621976501637462 2203 8 0.5663782593840025 2203 9 0.7590792560393851 2203 10-11 0.7235966825456188 2203 12-13 0.7532829417591955 2203 14-15 0.9519911891465043 2203 16-17 0.9208474783659142 2203 18-19 0.9228681052222001 2203 20-21 0.8997496329939736 2203 22-23 0.8756102114419022 2203 24-25 0.9255525527855823 2203 26-27 0.9367555387868549 2203 28-29 0.9781392809947604 2203 30-31 1.05268052697798 2203 32-33 1.1429965818955736 2203 34-35 1.1163931792303217 2203 36-37 1.131018190737656 2203 38-39 1.1304314240045201 2203 40-41 1.0806514046527766 2203 42-43 1.1562548504966799 2203 44-45 1.1520644921539471 2203 46-47 1.1983667570696 2203 48-49 1.1469092234699545 2203 50-51 1.129153256133499 2203 52-53 1.201114995350693 2203 54-55 1.2191209382026003 2203 56-57 1.2614611761914958 2203 58-59 1.294656408168116 2203 60-61 1.2920377217127168 2203 62-63 1.24707794485564 2203 64-65 1.2479721847446612 2203 66-67 1.266430334613581 2203 68-69 1.244944939311381 2203 70-71 1.2681169965027763 2203 72-73 1.2855864631483271 2203 74-75 1.3033225545492186 2203 76-77 1.3114836429086267 2203 78-79 1.0978747291875095 2203 80-81 1.08404909087238 2203 82-83 1.0831834470632522 2203 84-85 1.070308623406067 2203 86-87 1.0527093251039794 2203 88-89 1.0327763434277237 2203 90-91 1.0757512291641262 2203 92-93 1.0372798602001225 2203 94-95 1.0099506338563309 2203 96-97 0.9977450992614827 2203 98-99 1.0163979596789865 2203 100 1.2164058040256442 2204 1 0.728062822062423 2204 2 0.7262345876985172 2204 3 0.6891758579006435 2204 4 0.456231297777137 2204 5 0.5676608346793017 2204 6 0.5671918991108384 2204 7 0.6207599699377937 2204 8 0.6068512105819721 2204 9 0.7997597838666408 2204 10-11 0.7568261900018385 2204 12-13 0.8178341106250855 2204 14-15 1.017673260232037 2204 16-17 0.9836467813598233 2204 18-19 0.9908250277129724 2204 20-21 0.9381761845615415 2204 22-23 0.9617411099852049 2204 24-25 0.97562717184055 2204 26-27 0.953410508339033 2204 28-29 0.9903652791248234 2204 30-31 1.105852719532237 2204 32-33 1.174532589172017 2204 34-35 1.1665355945562723 2204 36-37 1.20460942051038 2204 38-39 1.209907155113342 2204 40-41 1.228750196774655 2204 42-43 1.2637891428783163 2204 44-45 1.241843178528029 2204 46-47 1.2484207207594409 2204 48-49 1.296997296284509 2204 50-51 1.329263907991404 2204 52-53 1.258844375238347 2204 54-55 1.2572295354091167 2204 56-57 1.3112384283184042 2204 58-59 1.3198860475565297 2204 60-61 1.2810175469554608 2204 62-63 1.3143915700056112 2204 64-65 1.3299960102867487 2204 66-67 1.314971825276082 2204 68-69 1.33637169924679 2204 70-71 1.390208202411337 2204 72-73 1.4079113715862022 2204 74-75 1.3973013729577914 2204 76-77 1.31413888629924 2204 78-79 1.1690036468297578 2204 80-81 1.1008081444437856 2204 82-83 1.065012587896934 2204 84-85 1.110642589451313 2204 86-87 1.098693158886153 2204 88-89 1.1139834049236335 2204 90-91 1.206042850791782 2204 92-93 1.1698122352971438 2204 94-95 1.1852288455206512 2204 96-97 1.1298243030802304 2204 98-99 1.162107238943328 2204 100 1.3936946030970638 2205 1 0.7445479929608361 2205 2 0.5601629600133471 2205 3 0.6347645448224029 2205 4 0.4663021038834714 2205 5 0.5591355221758576 2205 6 0.596108108938104 2205 7 0.644440695976165 2205 8 0.6536964682994437 2205 9 0.8447974428857137 2205 10-11 0.7988257781431329 2205 12-13 0.8392653112988455 2205 14-15 1.0268218160644267 2205 16-17 1.0112044490062075 2205 18-19 0.9857906824899132 2205 20-21 0.9854327587025935 2205 22-23 1.0102620889749687 2205 24-25 1.0284416884652359 2205 26-27 1.07891005142136 2205 28-29 1.1003342837862036 2205 30-31 1.1875740744496142 2205 32-33 1.2210513821883282 2205 34-35 1.1981812561922283 2205 36-37 1.2219586228381232 2205 38-39 1.2548499683977639 2205 40-41 1.292356123302575 2205 42-43 1.3277884962806183 2205 44-45 1.2996854508961846 2205 46-47 1.3042518767003486 2205 48-49 1.343403514409978 2205 50-51 1.3158925745747112 2205 52-53 1.3495072224280165 2205 54-55 1.3267109329397115 2205 56-57 1.3704733091976067 2205 58-59 1.3765815557127894 2205 60-61 1.3978028441129595 2205 62-63 1.4179422019590433 2205 64-65 1.4456198118979486 2205 66-67 1.4432909724966763 2205 68-69 1.4556286431777536 2205 70-71 1.4762488418610147 2205 72-73 1.4635944255840272 2205 74-75 1.4575405527745318 2205 76-77 1.4040344744198237 2205 78-79 1.3582177235132136 2205 80-81 1.2658090552087806 2205 82-83 1.2177058821560856 2205 84-85 1.212867179812715 2205 86-87 1.263435924735493 2205 88-89 1.292202928225361 2205 90-91 1.2889705559251468 2205 92-93 1.3104164943563887 2205 94-95 1.3643318058967715 2205 96-97 1.342661740477407 2205 98-99 1.419852712982852 2205 100 1.6292333380591977 2206 1 0.7640277149388375 2206 2 0.7405311286033474 2206 3 0.7166844446423042 2206 4 0.4035418582888468 2206 5 0.56516121611795 2206 6 0.5785022038035663 2206 7 0.6341944629175273 2206 8 0.644577297327416 2206 9 0.8372196198415054 2206 10-11 0.8348235135190336 2206 12-13 0.866650140898102 2206 14-15 1.0652502768735914 2206 16-17 1.0555514801346177 2206 18-19 1.0407896525783897 2206 20-21 1.0347247863609468 2206 22-23 1.0424719196966947 2206 24-25 1.1122312707826296 2206 26-27 1.1342107950160027 2206 28-29 1.1741435343029991 2206 30-31 1.1679544297040394 2206 32-33 1.227758412596586 2206 34-35 1.284167428711811 2206 36-37 1.3164518225027493 2206 38-39 1.2969088368419364 2206 40-41 1.2787833617253241 2206 42-43 1.3476837940907558 2206 44-45 1.3549895375923668 2206 46-47 1.327510574645217 2206 48-49 1.354885363793045 2206 50-51 1.3268520357505977 2206 52-53 1.4042938408824739 2206 54-55 1.3989305752425523 2206 56-57 1.417333195807629 2206 58-59 1.3996624205312642 2206 60-61 1.3843093794789496 2206 62-63 1.4540203423954026 2206 64-65 1.4847101336850272 2206 66-67 1.5140607437294165 2206 68-69 1.5570308487075195 2206 70-71 1.5680589203645 2206 72-73 1.5494313866512641 2206 74-75 1.5089147326885737 2206 76-77 1.4388607892705565 2206 78-79 1.2893471339458387 2206 80-81 1.2547010650757535 2206 82-83 1.271637559113298 2206 84-85 1.2734738961528116 2206 86-87 1.3217018793856603 2206 88-89 1.4291745433884095 2206 90-91 1.3743777268850508 2206 92-93 1.4262465679594847 2206 94-95 1.4561745747957318 2206 96-97 1.4193667506623555 2206 98-99 1.4349229815457285 2206 100 1.6368621382454833 2207 1 0.7236910953391096 2207 2 0.7138001636373055 2207 3 0.664782950985483 2207 4 0.45420979776688597 2207 5 0.5529677177727095 2207 6 0.5941658137265478 2207 7 0.6290208806298949 2207 8 0.6427961119740502 2207 9 0.8359509077964518 2207 10-11 0.8309838177717879 2207 12-13 0.8347355589180552 2207 14-15 1.056580647701871 2207 16-17 1.020470864678451 2207 18-19 0.9979036126684306 2207 20-21 0.994882694171281 2207 22-23 1.0314720284897447 2207 24-25 1.0653687763531465 2207 26-27 1.0785677902030884 2207 28-29 1.1065641209463308 2207 30-31 1.1866837034861817 2207 32-33 1.2288058633025827 2207 34-35 1.2247235444925053 2207 36-37 1.2415207808369004 2207 38-39 1.2859637445175736 2207 40-41 1.347909855317134 2207 42-43 1.3652725477342287 2207 44-45 1.3845823498924048 2207 46-47 1.3948607726473057 2207 48-49 1.4319226827895548 2207 50-51 1.4007823244189197 2207 52-53 1.415660686031785 2207 54-55 1.4444770973628565 2207 56-57 1.4447699967581684 2207 58-59 1.3613621388086088 2207 60-61 1.3717095352554551 2207 62-63 1.487038448096719 2207 64-65 1.4966860049498223 2207 66-67 1.5017763702911395 2207 68-69 1.528456666380869 2207 70-71 1.5613621292021236 2207 72-73 1.5398750915159027 2207 74-75 1.519392695181395 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1.50159114684093 2311 60-61 1.4756288982454109 2311 62-63 1.533076802799478 2311 64-65 1.594903256869486 2311 66-67 1.623730642367569 2311 68-69 1.6079241319626831 2311 70-71 1.6402594241660182 2311 72-73 1.6268866893927338 2311 74-75 1.5241907864764244 2311 76-77 1.3572084885129811 2311 78-79 1.340240375075787 2311 80-81 1.3161011586201177 2311 82-83 1.3599016348840145 2311 84-85 1.4168579075186791 2311 86-87 1.4747523853055249 2311 88-89 1.4775048842559464 2311 90-91 1.5617310051990358 2311 92-93 1.581811544790142 2311 94-95 1.645027232575913 2311 96-97 1.7424550215129564 2311 98-99 1.7648736083693777 2311 100 1.8577115349328324 2312 1 0.20540822772767697 2312 2 0.22967062519790105 2312 3 0.40863664297982183 2312 4 0.31210881225312903 2312 5 0.40004355369684674 2312 6 0.4717012618016909 2312 7 0.5068823059187153 2312 8 0.3838517055210673 2312 9 0.4703768729855824 2312 10-11 0.3961626268628322 2312 12-13 0.5525707198610661 2312 14-15 0.8845666713942606 2312 16-17 0.9424460679651645 2312 18-19 0.9636571117998329 2312 20-21 0.9721754664391398 2312 22-23 0.6214950682884677 2312 24-25 0.613181897189996 2312 26-27 0.9098183894473095 2312 28-29 1.0708081432527479 2312 30-31 1.031004416972067 2312 32-33 0.8467202565771785 2312 34-35 1.0222428746356016 2312 36-37 1.1040606475444648 2312 38-39 1.0947147072565855 2312 40-41 1.1086813134713651 2312 42-43 1.0087768109324955 2312 44-45 0.9350300201713182 2312 46-47 1.073656989087496 2312 48-49 1.1678685744148183 2312 50-51 1.2176332568804114 2312 52-53 1.2469390723044071 2312 54-55 1.2874581201907134 2312 56-57 1.2634751238550024 2312 58-59 1.2820417882716768 2312 60-61 1.3030929074423483 2312 62-63 1.3117315124209554 2312 64-65 1.391661965385751 2312 66-67 1.379109721955082 2312 68-69 1.345627022305628 2312 70-71 1.1613520260190953 2312 72-73 1.2960019311347182 2312 74-75 1.3439915397912259 2312 76-77 1.265461993550943 2312 78-79 1.1833451206848054 2312 80-81 1.0654382717371753 2312 82-83 1.0738286932617207 2312 84-85 0.9437102987237758 2312 86-87 1.1596207319654361 2312 88-89 0.8675192519394166 2312 90-91 1.2045501775173655 2312 92-93 1.3680067049627347 2312 94-95 1.4767320204531806 2312 96-97 1.554605317205354 2312 98-99 1.6039883882720147 2312 100 1.6970837219365258 2313 1 -0.25974499766423165 2313 2 0.0866921812003163 2313 3 0.3228465808559733 2313 4 0.1602637234780957 2313 5 0.30577272373228226 2313 6 0.43449199892827295 2313 7 0.2156534094701641 2313 8 0.19754835735548681 2313 9 0.3548847902109671 2313 10-11 0.49829578540580854 2313 12-13 0.6913893006940697 2313 14-15 0.7354922632798022 2313 16-17 0.8775401402645002 2313 18-19 0.7769976240938234 2313 20-21 0.9647026211571159 2313 22-23 1.0366620779883746 2313 24-25 0.8640739800724049 2313 26-27 1.0708356387541826 2313 28-29 1.0045707596250466 2313 30-31 1.0159685674167065 2313 32-33 1.1705812185545028 2313 34-35 1.1656100324310046 2313 36-37 1.0380188229355554 2313 38-39 0.8683011610803817 2313 40-41 1.012842422727168 2313 42-43 1.0238418092354848 2313 44-45 1.2485274463439922 2313 46-47 1.208152253912445 2313 48-49 1.2455110544861512 2313 50-51 1.1946727139018023 2313 52-53 1.1300134988017305 2313 54-55 1.1959435241716463 2313 56-57 1.2218390775576538 2313 58-59 1.1922850354926595 2313 60-61 1.2283649617110513 2313 62-63 1.2932091322252859 2313 64-65 1.3601232970401185 2313 66-67 1.3848450997517858 2313 68-69 1.3765029644413787 2313 70-71 1.2899985556245142 2313 72-73 1.358698532699968 2313 74-75 1.0287072741121435 2313 76-77 1.1418966934910344 2313 78-79 0.9867386015723625 2313 80-81 1.087974214625259 2313 82-83 1.0901614067692726 2313 84-85 1.1818497442331726 2313 86-87 1.306689348088998 2313 88-89 1.2475652370320454 2313 90-91 1.3811622373031867 2313 92-93 1.1341399942282706 2313 94-95 1.179558350399187 2313 96-97 1.4850665293995 2313 98-99 1.6204506470409505 2313 100 1.5612842882420814 2314 1 -0.26469880927205836 2314 2 -0.06298427826315844 2314 3 0.2070430490975923 2314 4 -0.06475551838070004 2314 5 0.26995113866061615 2314 6 0.24722936365434833 2314 7 0.18914379388379388 2314 8 0.26277494015664615 2314 9 0.34521053836432714 2314 10-11 0.5169451402852374 2314 12-13 0.6049901074829904 2314 14-15 0.6816732075615377 2314 16-17 0.8784738379311392 2314 18-19 0.9522647365471499 2314 20-21 0.5946476355524055 2314 22-23 0.792147931374906 2314 24-25 0.9643828951126352 2314 26-27 0.9941068553489174 2314 28-29 0.9779626893509814 2314 30-31 0.9546575112135685 2314 32-33 1.007642924659116 2314 34-35 1.0592680749491592 2314 36-37 0.9372424311866112 2314 38-39 0.9755746682341098 2314 40-41 1.0690711894076443 2314 42-43 0.8955200057814565 2314 44-45 0.9214234960873711 2314 46-47 1.0214652568841132 2314 48-49 0.9257546777210663 2314 50-51 1.052525741345093 2314 52-53 1.146196013015441 2314 54-55 1.2133811317244394 2314 56-57 1.1070415674060428 2314 58-59 1.1332564976551964 2314 60-61 1.1750791617798555 2314 62-63 0.934573789437458 2314 64-65 1.0502397320614563 2314 66-67 1.121738447940409 2314 68-69 1.2079723461901253 2314 70-71 1.2761748683773462 2314 72-73 1.2733367339939932 2314 74-75 1.3093269411368524 2314 76-77 1.0218398781774312 2314 78-79 1.0580747823919054 2314 80-81 1.0851741217038757 2314 82-83 1.0694845813779388 2314 84-85 0.9495290281549593 2314 86-87 1.072587072983044 2314 88-89 1.1497885938825014 2314 90-91 1.2820874296062925 2314 92-93 1.3525942701369047 2314 94-95 1.419022543942262 2314 96-97 1.5174933383304143 2314 98-99 1.548440628960364 2314 100 1.5409226615148306 2315 1 0.05630783084321678 2315 2 0.23126693522906194 2315 3 0.22338985293332314 2315 4 0.036087383709634935 2315 5 0.3253858770986824 2315 6 0.4455445155975042 2315 7 0.4943265090963962 2315 8 0.45811727454588436 2315 9 0.6612578575161336 2315 10-11 0.666454234016399 2315 12-13 0.7556199767070382 2315 14-15 0.8687536385205163 2315 16-17 0.85758397876684 2315 18-19 0.911847693727033 2315 20-21 0.8896306578509794 2315 22-23 1.0069511848220074 2315 24-25 0.9007659982959879 2315 26-27 0.9874954409483792 2315 28-29 1.0592061682715794 2315 30-31 1.0444524178815158 2315 32-33 1.0757333982438269 2315 34-35 1.0753792076449287 2315 36-37 1.1163347152257685 2315 38-39 1.1026157493780318 2315 40-41 1.0953086532763407 2315 42-43 1.1469303699393336 2315 44-45 0.9910856248162219 2315 46-47 0.9869898819100058 2315 48-49 1.1065552986840217 2315 50-51 1.0622935398089908 2315 52-53 1.084497223808313 2315 54-55 1.0062270902811505 2315 56-57 1.1151083079793196 2315 58-59 1.1738810615588307 2315 60-61 1.1866028545472602 2315 62-63 1.2184334795213516 2315 64-65 1.2805642522610299 2315 66-67 1.2833982917460887 2315 68-69 1.1794271782740324 2315 70-71 0.8227895532293061 2315 72-73 1.0906642809912839 2315 74-75 1.238945542002277 2315 76-77 1.131785516703438 2315 78-79 1.0044726186335708 2315 80-81 1.0105225194488234 2315 82-83 1.050322241672088 2315 84-85 1.030484521959952 2315 86-87 1.0338797209663149 2315 88-89 1.1523569137434428 2315 90-91 1.2009998596145337 2315 92-93 1.2450403173749613 2315 94-95 1.349167781657453 2315 96-97 1.382343402206935 2315 98-99 1.3254413420139066 2315 100 1.2271690237184707 2316 1 0.535495118637531 2316 2 0.532335906239016 2316 3 0.5270747157347913 2316 4 0.3856341588941916 2316 5 0.4589952516694211 2316 6 0.5035933271620223 2316 7 0.5414967273226097 2316 8 0.5592790290176666 2316 9 0.5964719384761992 2316 10-11 0.5888845630665074 2316 12-13 0.7258517465435048 2316 14-15 0.9121170242367498 2316 16-17 0.9006338126161637 2316 18-19 0.9458091736131351 2316 20-21 0.9768547891312096 2316 22-23 0.9879794165774101 2316 24-25 1.0318167469875519 2316 26-27 1.0952318517919508 2316 28-29 1.0629456409165883 2316 30-31 0.9373904832735604 2316 32-33 0.9127700397958307 2316 34-35 0.8613423822729516 2316 36-37 0.8401281745597444 2316 38-39 0.7938112008883635 2316 40-41 0.8546959332450612 2316 42-43 0.9661702348687058 2316 44-45 0.9106384728956627 2316 46-47 0.9964975231367106 2316 48-49 0.772540399865953 2316 50-51 0.7949939584867423 2316 52-53 0.975488023642697 2316 54-55 1.0521741730678187 2316 56-57 1.0796625336973236 2316 58-59 0.9413321155899652 2316 60-61 1.050894606301199 2316 62-63 1.1289365548533894 2316 64-65 1.1129113195199167 2316 66-67 1.114939887637668 2316 68-69 1.0751786334366855 2316 70-71 0.923877297379974 2316 72-73 0.9695543393320278 2316 74-75 1.045688274255248 2316 76-77 0.8607170241835149 2316 78-79 0.8118489353870331 2316 80-81 0.8356646847757752 2316 82-83 0.7480310453752175 2316 84-85 0.8484977102532874 2316 86-87 0.8605756676716716 2316 88-89 0.8652569430513815 2316 90-91 0.9306380500495521 2316 92-93 0.9769826063345803 2316 94-95 0.9192408784664217 2316 96-97 1.10069222051035 2316 98-99 1.2159267980250945 2316 100 0.8308500429676542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 15.0 12 86.0 13 407.0 14 1614.0 15 4542.0 16 10387.0 17 21070.0 18 33766.0 19 46806.0 20 63558.0 21 84814.0 22 100230.0 23 107126.0 24 116852.0 25 137016.0 26 166894.0 27 207845.0 28 255214.0 29 311189.0 30 383518.0 31 473924.0 32 596307.0 33 769378.0 34 1049333.0 35 1412535.0 36 2068366.0 37 2897184.0 38 2292785.0 39 295221.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74447708935714 20.428018111380297 13.257906176305262 24.5695986229573 2 15.57604527579946 20.994349728400998 39.95748778904579 23.47211720675375 3 17.662843640252046 27.213315440518997 30.707166894688843 24.416674024540114 4 12.177559940193746 17.3000040487376 39.010794883690124 31.511641127378535 5 12.652479744207138 38.40407584308988 35.04608267925594 13.897361733447047 6 29.836689721975922 38.11011989930403 18.65188563362507 13.401304745094983 7 26.556326727034367 32.73398476228303 23.50341950946323 17.206269001219372 8 25.90024493159136 35.76177391875807 20.484075132487032 17.853906017163546 9 27.102301296160253 14.844765563079806 20.538992193644834 37.5139409471151 10-11 24.958483997213094 25.988367034826993 28.916481939388362 20.13666702857155 12-13 25.332636658352968 23.89356949915329 28.61554973379289 22.158244108700853 14-15 22.796351206757294 25.23583287038021 26.12962809773702 25.83818782512548 16-17 22.26435028585548 27.658897067760947 27.009511331598286 23.06724131478529 18-19 21.86920207960826 27.451739300621426 28.658706166607374 22.020352453162943 20-21 22.976609757815293 26.693758027978216 28.07233146130531 22.25730075290118 22-23 22.856667488495653 26.55911492109974 27.880156482566186 22.704061107838417 24-25 22.38374402636757 26.619735626882235 28.395962178588647 22.600558168161548 26-27 22.495933546172427 26.747795019450134 27.994857636817365 22.761413797560074 28-29 22.191991572551302 27.286068950879056 28.037930134100534 22.484009342469108 30-31 23.09587303833373 26.774660816459274 28.015313329857605 22.114152815349396 32-33 23.06421635173596 26.793841545770604 27.689381621861376 22.452560480632062 34-35 23.01850041122573 26.83961304795217 28.13864549522998 22.003241045592116 36-37 22.771964396957802 26.763071224316953 27.99714924793937 22.467815130785876 38-39 22.71115310998419 26.77636714781985 28.148263213553843 22.364216528642118 40-41 22.64905308685711 26.224284459405002 28.544038850786468 22.582623602951426 42-43 22.460111542832788 26.521598405033338 28.025051353556325 22.993238698577553 44-45 22.07290569591861 27.021113128792955 28.20594223383303 22.7000389414554 46-47 22.169253880101046 26.456133626631555 27.983077344582025 23.39153514868537 48-49 22.45527493221443 26.41600814135136 28.060313187915664 23.068403738518555 50-51 22.284791047500576 26.608922794191535 27.798498293063783 23.3077878652441 52-53 21.962807102550947 26.84593222841872 27.71993507964192 23.471325589388407 54-55 21.879914907031676 26.68393271948889 27.88540637184799 23.55074600163145 56-57 22.32402135017015 26.421426962598016 27.586504302408375 23.66804738482346 58-59 21.850758544378866 26.522990532783417 28.04763313113777 23.57861779169995 60-61 22.24318331856881 26.920778955628094 27.456310320798604 23.379727405004488 62-63 22.181886174668108 26.676787787814817 27.31173001606751 23.829596021449568 64-65 21.631386696094918 26.442551318965858 27.447786061854774 24.47827592308445 66-67 21.819198608204164 26.40025682563543 27.278052521648082 24.50249204451232 68-69 21.537677354294654 26.536182114136498 27.423384318732687 24.50275621283616 70-71 21.717435510800897 26.238468201192244 27.457454928613245 24.58664135939361 72-73 21.796147278677907 26.608094668679367 27.234713124630982 24.36104492801174 74-75 21.953185573364152 26.71566107588984 26.91944784178955 24.41170550895646 76-77 21.74674419010251 26.569066538072267 26.94870050792271 24.735488763902516 78-79 21.677626244885 26.50444525382369 27.02183274541479 24.796095755876518 80-81 21.575372888867303 26.929539433449577 26.889015205537326 24.606072472145797 82-83 21.948140820323946 26.525905316919808 27.14022590715087 24.385727955605372 84-85 22.08561999623428 26.677423131350498 26.588002525587218 24.64895434682801 86-87 21.53244101131587 26.939992571396367 26.792218963900776 24.735347453386986 88-89 21.755332326125867 26.38219296721902 26.84459450199399 25.017880204661125 90-91 21.952441877555547 26.230556879265727 26.824746610945333 24.992254632233397 92-93 21.97398334960553 26.372561503137575 26.677863444499337 24.97559170275755 94-95 21.38377652865787 26.17596948767516 27.05751247066593 25.382741513001044 96-97 21.44524155817729 26.057515503811175 27.007843088614088 25.489399849397454 98-99 21.315587016983095 26.256043975999035 26.88478828351891 25.543580723498966 100 21.406610169869385 26.102988846507863 26.79030078464152 25.70010019898124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 500.0 1 398.5 2 638.0 3 965.5 4 756.0 5 647.5 6 935.5 7 1711.0 8 2781.0 9 3454.0 10 3515.0 11 3587.0 12 4150.5 13 4991.0 14 5991.0 15 7099.5 16 8164.0 17 9272.5 18 10671.0 19 12165.5 20 13721.0 21 15794.5 22 18671.0 23 22334.5 24 27351.0 25 34241.5 26 44146.5 27 56299.0 28 71131.0 29 93811.0 30 122479.5 31 150561.5 32 187606.0 33 225273.5 34 259271.0 35 296301.5 36 335272.5 37 383418.5 38 411205.5 39 413914.0 40 413106.0 41 414852.5 42 429063.5 43 451625.5 44 478687.0 45 520063.0 46 557621.5 47 587720.5 48 617411.0 49 626579.0 50 624404.0 51 621506.0 52 599396.0 53 562925.5 54 526149.0 55 471291.0 56 412372.0 57 351504.5 58 292032.0 59 248801.0 60 200556.5 61 154276.0 62 120523.0 63 93622.5 64 69668.5 65 48650.0 66 34764.5 67 26742.0 68 20770.5 69 15894.0 70 12518.0 71 9612.5 72 7438.0 73 5919.5 74 4610.0 75 3443.0 76 2447.5 77 1581.0 78 998.0 79 649.0 80 441.0 81 297.0 82 188.5 83 110.0 84 71.0 85 57.5 86 32.0 87 23.0 88 16.0 89 8.5 90 4.5 91 3.0 92 4.0 93 2.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049683621028581815 2 0.0 3 0.0 4 0.017507904081707198 5 0.0036022422771808233 6 0.0 7 1.0785156518505458E-4 8 2.8041406948114197E-4 9 0.0016752943125411814 10-11 8.196718954064149E-4 12-13 2.58843756444131E-4 14-15 6.11158869381976E-5 16-17 1.0425651301221944E-4 18-19 0.0 20-21 2.5165365209846072E-5 22-23 7.190104345670306E-6 24-25 6.471093911103275E-5 26-27 1.0785156518505458E-5 28-29 8.44837260616261E-4 30-31 8.70002625826107E-4 32-33 0.014250786813118548 34-35 0.02323841724520643 36-37 0.030949804155937833 38-39 0.02062840936772811 40-41 1.6537239995041702E-4 42-43 0.0018155013472817521 44-45 0.008847423397347311 46-47 0.012899047196132529 48-49 0.0052523712245121585 50-51 0.009796517170975792 52-53 0.014685788126031598 54-55 0.009577218988432848 56-57 0.0027789753296015734 58-59 0.008419612188779929 60-61 0.001642938842985665 62-63 8.951679910359532E-4 64-65 0.018302410611903762 66-67 0.042888972421923376 68-69 0.051962884106159304 70-71 0.04554931102982139 72-73 0.03800329651904041 74-75 0.02706714780927587 76-77 0.027476983756979077 78-79 0.011496976848726819 80-81 0.001973683642886499 82-83 0.026020987626980834 84-85 0.045200590969056376 86-87 0.045538525873302885 88-89 0.05648186468741309 90-91 0.03258914794675066 92-93 0.024507470662217236 94-95 0.006960021006608856 96-97 0.0038574909814521195 98-99 0.022623663323651618 100 0.019219148915976727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3908004E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.147425630825275 #Duplication Level Percentage of deduplicated Percentage of total 1 70.7236477269523 38.295034556358345 2 15.97830643062185 17.303683183172673 3 5.927599933965636 9.628928297810672 4 2.7102671686957045 5.870159598264721 5 1.4385583525030785 3.8947115703881483 6 0.8573795598540884 2.7854937572753373 7 0.5473312209098236 2.0745603605750427 8 0.3726860839563161 1.6143993611734502 9 0.24162712323136554 1.1775138017004556 >10 1.1170132123075858 10.31091798711799 >50 0.05413126364144517 2.0088510478852135 >100 0.028903492903858376 2.932407715960814 >500 0.0016735282340176244 0.6293546755250675 >1k 7.971332414335799E-4 0.7207748085630498 >5k 1.944224534858726E-5 0.09768444368996364 >10k+ 5.832673604576178E-5 0.6555248345389219 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25884 0.1861086608833302 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21482 0.15445782155368953 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14902 0.10714693495917889 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.4380208691340613E-5 0.0 0.0 0.0 0.0 7 1.4380208691340613E-5 0.0 0.0 0.0 0.0 8 1.4380208691340613E-5 0.0 0.0 0.0 0.0 9 1.4380208691340613E-5 0.0 0.0 1.4380208691340613E-5 7.190104345670306E-6 10-11 1.4380208691340613E-5 0.0 0.0 3.5950521728351534E-5 7.190104345670306E-6 12-13 1.4380208691340613E-5 0.0 0.0 7.549609562953822E-5 7.190104345670306E-6 14-15 1.4380208691340613E-5 0.0 0.0 1.0066146083938429E-4 7.190104345670306E-6 16-17 2.157031303701092E-5 0.0 0.0 1.617773477775819E-4 1.4380208691340613E-5 18-19 3.5950521728351534E-5 0.0 0.0 2.1929818254294434E-4 1.4380208691340613E-5 20-21 3.5950521728351534E-5 0.0 0.0 4.278112085673832E-4 1.4380208691340613E-5 22-23 3.5950521728351534E-5 0.0 0.0 0.0010677304953320404 2.5165365209846072E-5 24-25 3.5950521728351534E-5 0.0 0.0 0.0024338503210093983 3.5950521728351534E-5 26-27 3.5950521728351534E-5 0.0 0.0 0.0045944766768833255 4.314062607402184E-5 28-29 3.5950521728351534E-5 0.0 0.0 0.013362808926428264 4.314062607402184E-5 30-31 3.954557390118669E-5 0.0 0.0 0.0497123814459645 4.314062607402184E-5 32-33 4.673567824685699E-5 0.0 0.0 0.13025593032616326 4.314062607402184E-5 34-35 5.0330730419692145E-5 0.0 0.0 0.24352523913568042 4.314062607402184E-5 36-37 5.0330730419692145E-5 0.0 0.0 0.43056142347960213 4.673567824685699E-5 38-39 5.0330730419692145E-5 0.0 0.0 0.7522035512788177 5.0330730419692145E-5 40-41 5.0330730419692145E-5 0.0 0.0 1.152771454480456 6.83059912838679E-5 42-43 5.0330730419692145E-5 0.0 0.0 1.5281883726809395 7.190104345670307E-5 44-45 5.39257825925273E-5 0.0 0.0 1.9328330650465733 7.549609562953822E-5 46-47 5.752083476536245E-5 0.0 0.0 2.3926294527956706 8.628125214804368E-5 48-49 5.752083476536245E-5 0.0 0.0 2.9000494966783155 9.347135649371398E-5 50-51 5.752083476536245E-5 0.0 0.0 3.4570812605460857 9.347135649371398E-5 52-53 5.752083476536245E-5 0.0 0.0 4.005287171329545 1.0425651301221944E-4 54-55 5.752083476536245E-5 0.0 0.0 4.574775790976188 1.1863672170356004E-4 56-57 5.752083476536245E-5 0.0 0.0 5.184036472810908 1.222317738763952E-4 58-59 7.909114780237337E-5 0.0 0.0 5.866434896049785 1.294218782220655E-4 60-61 7.909114780237337E-5 0.0 0.0 6.557044418451419 1.4380208691340614E-4 62-63 7.909114780237337E-5 0.0 0.0 7.2541681753902285 1.5818229560474675E-4 64-65 7.909114780237337E-5 0.0 0.0 7.914129878018442 1.5818229560474675E-4 66-67 7.909114780237337E-5 0.0 0.0 8.612425621965595 1.689674521232522E-4 68-69 7.909114780237337E-5 0.0 0.0 9.375195031580375 1.7256250429608736E-4 70-71 7.909114780237337E-5 0.0 0.0 10.186192066093739 1.7256250429608736E-4 72-73 7.909114780237337E-5 0.0 0.0 10.967098513920474 1.7975260864175766E-4 74-75 8.268619997520853E-5 0.0 0.0 11.699033161048845 1.7975260864175766E-4 76-77 8.628125214804368E-5 0.0 0.0 12.474870585311882 1.7975260864175766E-4 78-79 8.628125214804368E-5 0.0 0.0 13.307765082609984 1.7975260864175766E-4 80-81 8.628125214804368E-5 0.0 0.0 14.146785548810598 1.7975260864175766E-4 82-83 8.628125214804368E-5 0.0 0.0 14.982113896429711 1.8694271298742797E-4 84-85 8.628125214804368E-5 0.0 0.0 15.784080159884912 1.8694271298742797E-4 86-87 8.628125214804368E-5 0.0 0.0 16.569839928144972 1.8694271298742797E-4 88 8.628125214804368E-5 0.0 0.0 17.16551850287072 1.8694271298742797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18730 0.0 47.221916 1 GTATCAA 32985 0.0 39.45865 1 CAACGCA 40690 0.0 31.144829 5 ATCAACG 41035 0.0 30.876968 3 TACCTGG 9380 0.0 30.6129 2 TATCAAC 43620 0.0 29.574865 2 AACGCAG 44560 0.0 29.028986 6 TCAACGC 44590 0.0 28.56333 4 GTACATG 51935 0.0 26.95326 1 TACATGG 51605 0.0 26.610592 2 GTACTAG 6890 0.0 26.001497 1 ACGCAGA 49280 0.0 25.724213 7 ACATGGG 51430 0.0 25.485971 3 CGCAGAG 50620 0.0 25.00603 8 ACCTGGG 11265 0.0 24.86476 3 CATGGGG 38715 0.0 23.744698 4 TGGGGGG 27775 0.0 23.192856 6 ATGGGGG 29465 0.0 23.17138 5 GCAGAGT 56570 0.0 21.968845 9 TAGGCAT 8935 0.0 21.035755 5 >>END_MODULE