FastQCFastQC Report
Mon 6 Jun 2016
ERR1211367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7990371
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272530.34107302401853434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218500.27345413623472553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139150.174147107812641No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115050.052.164161
GTATCAA213450.041.690121
TACCTGG67800.033.296452
ATCAACG263250.032.9514353
CAACGCA263900.032.9056785
TATCAAC274300.031.9942722
TCAACGC272600.031.9072114
AACGCAG280900.031.282466
GTACATG279850.030.1176361
TACATGG287500.028.5786342
ACGCAGA318000.027.5158277
GTACCTG85250.027.254661
ACATGGG294700.027.0590633
CGCAGAG327400.026.7388348
GTGGTAT53350.025.6546881
ACCTGGG87950.025.0046633
CATGGGG218100.024.1953774
GCAGAGT360150.023.7604059
GTACTAG22700.023.2060031
ATGGGGG150800.022.9654225
Produced by FastQC (version 0.11.5)