##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7990371 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.279041986911498 31.0 31.0 34.0 28.0 34.0 2 31.27364987182698 31.0 31.0 34.0 28.0 34.0 3 31.330313823976383 31.0 31.0 34.0 28.0 34.0 4 34.93743569603964 37.0 35.0 37.0 32.0 37.0 5 34.98075421028636 37.0 35.0 37.0 32.0 37.0 6 35.046382577229515 37.0 35.0 37.0 32.0 37.0 7 34.975310908592355 37.0 35.0 37.0 32.0 37.0 8 34.97464160299941 37.0 35.0 37.0 32.0 37.0 9 36.5217666363677 39.0 37.0 39.0 32.0 39.0 10-11 36.44094667193801 39.0 37.0 39.0 32.0 39.0 12-13 36.34941037155846 39.0 37.0 39.0 32.0 39.0 14-15 37.462884201497026 40.0 37.0 41.0 31.0 41.0 16-17 37.4550637260773 40.0 37.0 41.0 31.0 41.0 18-19 37.44814008010391 40.0 37.0 41.0 31.0 41.0 20-21 37.40723290570613 40.0 37.0 41.0 31.0 41.0 22-23 37.27823357638838 40.0 37.0 41.0 31.0 41.0 24-25 37.16359215360588 40.0 36.0 41.0 31.0 41.0 26-27 37.01951824264481 40.0 36.0 41.0 30.0 41.0 28-29 36.8667508054382 39.0 36.0 41.0 30.0 41.0 30-31 36.67085408174414 39.0 36.0 41.0 30.0 41.0 32-33 36.52923537592935 39.0 35.0 41.0 30.0 41.0 34-35 36.368218634654134 39.0 35.0 41.0 29.0 41.0 36-37 36.26234664197695 39.0 35.0 40.5 28.5 41.0 38-39 36.10175053198406 39.0 35.0 40.0 28.0 41.0 40-41 35.928627156361074 38.5 35.0 40.0 27.0 41.0 42-43 35.77301898747881 38.0 35.0 40.0 27.0 41.0 44-45 35.63532769629846 38.0 34.5 40.0 26.5 41.0 46-47 35.46732066633702 38.0 34.0 40.0 25.5 41.0 48-49 35.22715903929868 38.0 34.0 40.0 24.5 41.0 50-51 34.563521706313765 37.5 33.5 39.5 24.0 40.5 52-53 34.813718286672795 38.0 33.5 40.0 24.5 40.5 54-55 35.172571461325134 38.0 34.0 40.0 25.0 41.0 56-57 35.074691463012165 38.0 34.0 40.0 25.0 41.0 58-59 34.85274388385721 38.0 33.5 40.0 25.0 41.0 60-61 34.57419048502254 37.0 33.0 40.0 24.0 41.0 62-63 34.28690351173932 37.0 33.0 40.0 24.0 41.0 64-65 34.00750772398428 36.0 33.0 39.5 23.0 41.0 66-67 33.78368232213498 36.0 32.5 39.0 23.0 41.0 68-69 33.435661748371885 35.5 32.0 39.0 22.5 41.0 70-71 33.08074036862619 35.0 32.0 38.5 22.0 40.0 72-73 32.708143339026435 35.0 31.0 37.5 21.5 40.0 74-75 32.33795445793443 35.0 31.0 37.0 20.5 39.0 76-77 31.97842878634797 35.0 31.0 36.5 20.0 39.0 78-79 31.61202708860452 35.0 30.5 36.0 20.0 39.0 80-81 31.27445746887097 34.0 30.0 35.5 19.5 37.5 82-83 30.959546684377983 34.0 30.0 35.0 18.5 37.0 84-85 30.656531467687795 34.0 29.5 35.0 18.0 36.5 86-87 30.394659834943837 34.0 29.0 35.0 18.0 36.0 88-89 30.13006817831112 34.0 29.0 35.0 17.0 36.0 90-91 29.868642832729545 34.0 29.0 35.0 16.0 35.5 92-93 29.621686214570012 34.0 29.0 35.0 15.0 35.0 94-95 29.387971597313815 34.0 27.5 35.0 12.0 35.0 96-97 29.173801504335657 34.0 27.0 35.0 11.0 35.0 98-99 28.92448141644487 33.5 26.5 35.0 10.0 35.0 100 27.26539606233553 31.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3728224741983297 1101 2 -0.39994078521470655 1101 3 -0.39790275606889125 1101 4 -0.272342204678246 1101 5 -0.3332641557534757 1101 6 -0.3755355313469906 1101 7 -0.4464167546296096 1101 8 -0.4908109059066206 1101 9 -0.5442580866003794 1101 10-11 -0.549198164545821 1101 12-13 -0.43772104203566187 1101 14-15 -0.5833401380216685 1101 16-17 -0.617797755931683 1101 18-19 -0.5997259696797457 1101 20-21 -0.6457986978667307 1101 22-23 -0.6931088244865862 1101 24-25 -0.6177869080682612 1101 26-27 -0.6791710731339151 1101 28-29 -0.7405618427110099 1101 30-31 -0.7300981614047046 1101 32-33 -0.8334376491965756 1101 34-35 -0.7450329113944107 1101 36-37 -0.8030005382064189 1101 38-39 -0.8002643584368911 1101 40-41 -0.7442370762008963 1101 42-43 -0.8431736779761749 1101 44-45 -0.9328536239773229 1101 46-47 -0.7981716303459834 1101 48-49 -0.7995297601703584 1101 50-51 -0.7834173600329493 1101 52-53 -0.6071244803529581 1101 54-55 -0.548127245244757 1101 56-57 -0.5433112861317042 1101 58-59 -0.6222906441140452 1101 60-61 -0.5917891907345307 1101 62-63 -0.5505527952654106 1101 64-65 -0.6576802908505002 1101 66-67 -0.5888443100802121 1101 68-69 -0.6469154593117352 1101 70-71 -0.6243732658674119 1101 72-73 -0.6667222683675327 1101 74-75 -0.6225363660054484 1101 76-77 -0.6949760032524708 1101 78-79 -0.6497881031000894 1101 80-81 -0.6840063867322606 1101 82-83 -0.8418929177777237 1101 84-85 -0.8186299340335843 1101 86-87 -0.9465263177051781 1101 88-89 -0.8822807646826938 1101 90-91 -0.8958252715662347 1101 92-93 -1.0788412403326895 1101 94-95 -1.0362537026035596 1101 96-97 -1.1320454977539498 1101 98-99 -1.1092902755099807 1101 100 -1.18787599799154 1102 1 -0.30131282500990864 1102 2 -0.3662568273021236 1102 3 -0.35252850718465467 1102 4 -0.24530671212083632 1102 5 -0.26656195912944014 1102 6 -0.2739980815252636 1102 7 -0.33442352402148856 1102 8 -0.30520365530198745 1102 9 -0.37677111425141163 1102 10-11 -0.3773403841084999 1102 12-13 -0.4087683342789106 1102 14-15 -0.48988964932892287 1102 16-17 -0.5158320766238305 1102 18-19 -0.4779317691661191 1102 20-21 -0.5471457195836393 1102 22-23 -0.6001523268677218 1102 24-25 -0.6143035697482659 1102 26-27 -0.5717571553639473 1102 28-29 -0.6520776005587692 1102 30-31 -0.6385352796134924 1102 32-33 -0.6703894281877822 1102 34-35 -0.7304648497094206 1102 36-37 -0.757216448147318 1102 38-39 -0.9062703237297356 1102 40-41 -0.8916265593335169 1102 42-43 -0.873595580636561 1102 44-45 -0.7539884944488122 1102 46-47 -0.8824982441540428 1102 48-49 -0.9369080544830695 1102 50-51 -0.8292631600179874 1102 52-53 -0.7495840260681703 1102 54-55 -0.698175397686974 1102 56-57 -0.7372792967034272 1102 58-59 -0.6796151136580093 1102 60-61 -0.8011059870543846 1102 62-63 -0.8406203021843979 1102 64-65 -0.8001350057381771 1102 66-67 -0.7063587090629326 1102 68-69 -0.6944571914283202 1102 70-71 -0.8298423859711335 1102 72-73 -0.8493875450025357 1102 74-75 -0.7791828055297216 1102 76-77 -0.8311423831861475 1102 78-79 -0.8210646259015952 1102 80-81 -0.8664862219231217 1102 82-83 -0.9482284701750601 1102 84-85 -0.9620748127330678 1102 86-87 -0.9953203249857054 1102 88-89 -1.0149966870902496 1102 90-91 -1.0255702350389484 1102 92-93 -0.9953997587958021 1102 94-95 -1.0556907709457448 1102 96-97 -1.0541222237618797 1102 98-99 -1.0516393355244382 1102 100 -1.070627762022756 1103 1 -0.44499703274927427 1103 2 -0.44018486415416547 1103 3 -0.41005430807647514 1103 4 -0.31477510476533865 1103 5 -0.21745655178382606 1103 6 -0.3248460007199867 1103 7 -0.34566606791199206 1103 8 -0.346226827448163 1103 9 -0.49581696565733324 1103 10-11 -0.5013655123470429 1103 12-13 -0.6476450027686411 1103 14-15 -0.6551595811061404 1103 16-17 -0.7184854827975826 1103 18-19 -0.6100427960222206 1103 20-21 -0.7180703803635495 1103 22-23 -0.694714182601885 1103 24-25 -0.6919081006291989 1103 26-27 -0.6890586902280731 1103 28-29 -0.722751398247091 1103 30-31 -0.697056028122212 1103 32-33 -0.7259410033332898 1103 34-35 -0.8409974361377195 1103 36-37 -0.8359234085228593 1103 38-39 -0.8485976294975259 1103 40-41 -0.7762613084428693 1103 42-43 -0.873668524802504 1103 44-45 -0.859918876752964 1103 46-47 -0.8358769584844765 1103 48-49 -0.9369769894215239 1103 50-51 -0.9442050753891422 1103 52-53 -0.7576649644824371 1103 54-55 -0.7419010377125943 1103 56-57 -0.7610189376862948 1103 58-59 -0.8407242021444716 1103 60-61 -0.7064141394132406 1103 62-63 -0.8004497446759231 1103 64-65 -0.8574577732981865 1103 66-67 -0.8697860279822791 1103 68-69 -0.7940103624841228 1103 70-71 -0.7890399050481918 1103 72-73 -0.9173178423672645 1103 74-75 -0.9116395553127532 1103 76-77 -0.8806345134832299 1103 78-79 -0.7786376513867701 1103 80-81 -0.8218171382650254 1103 82-83 -0.9504549422504667 1103 84-85 -0.9858357642189262 1103 86-87 -1.0085978922340146 1103 88-89 -1.0491517931872707 1103 90-91 -0.9455924468076375 1103 92-93 -1.0916132217384877 1103 94-95 -1.2644261103841252 1103 96-97 -1.2880616744847444 1103 98-99 -1.2977938001450617 1103 100 -1.3033061864244502 1104 1 -0.3813007631981016 1104 2 -0.31542437874706764 1104 3 -0.372015343042289 1104 4 -0.2963024182112335 1104 5 -0.20274228238641712 1104 6 -0.2241124520147224 1104 7 -0.2400768450275521 1104 8 -0.30045178674255624 1104 9 -0.3407437101302122 1104 10-11 -0.31663018064734416 1104 12-13 -0.3099478969324636 1104 14-15 -0.47204811221111953 1104 16-17 -0.46447049281005093 1104 18-19 -0.5121060696054087 1104 20-21 -0.5042333250246784 1104 22-23 -0.4584300614910575 1104 24-25 -0.5731994825962801 1104 26-27 -0.6098177711530326 1104 28-29 -0.5354191206187338 1104 30-31 -0.6033475049317047 1104 32-33 -0.6278501285298361 1104 34-35 -0.5628814252021854 1104 36-37 -0.6888164974646998 1104 38-39 -0.6121438101315206 1104 40-41 -0.745613567791608 1104 42-43 -0.6912837630992499 1104 44-45 -0.6118872961380859 1104 46-47 -0.7152054562195005 1104 48-49 -0.8154973119423019 1104 50-51 -0.7697860258721221 1104 52-53 -0.6267799742036217 1104 54-55 -0.6361888840618022 1104 56-57 -0.664541573397365 1104 58-59 -0.681362355031851 1104 60-61 -0.6101774712285888 1104 62-63 -0.6665825018017841 1104 64-65 -0.6114226093334523 1104 66-67 -0.58538985273983 1104 68-69 -0.6280816590707374 1104 70-71 -0.7570735334017868 1104 72-73 -0.8047381230352109 1104 74-75 -0.7593745460298784 1104 76-77 -0.776228527356114 1104 78-79 -0.8086583390347535 1104 80-81 -0.8064850750555301 1104 82-83 -0.9381705139574059 1104 84-85 -1.008175395509788 1104 86-87 -0.9263266992260242 1104 88-89 -0.9242219095913775 1104 90-91 -0.9617735371258433 1104 92-93 -0.925073443856629 1104 94-95 -0.9712671823472654 1104 96-97 -1.0234453821190925 1104 98-99 -1.0659501899418338 1104 100 -1.087437896018578 1105 1 -0.3895586188496196 1105 2 -0.4409832259621602 1105 3 -0.3634815652636547 1105 4 -0.29340742352493265 1105 5 -0.2397917389317925 1105 6 -0.2518232949329544 1105 7 -0.27356272175837404 1105 8 -0.2849618438892989 1105 9 -0.37322086218110684 1105 10-11 -0.5128078795884576 1105 12-13 -0.5233701321868836 1105 14-15 -0.5539367341383965 1105 16-17 -0.6425046413254805 1105 18-19 -0.6466109171657877 1105 20-21 -0.6221759041464097 1105 22-23 -0.6606085993356743 1105 24-25 -0.5746056831038402 1105 26-27 -0.5996585308334375 1105 28-29 -0.701028112315079 1105 30-31 -0.7696380286573188 1105 32-33 -0.7213545370291854 1105 34-35 -0.83807217173581 1105 36-37 -0.7387340400243545 1105 38-39 -0.6904064446015425 1105 40-41 -0.8398139965189841 1105 42-43 -0.8062032110012325 1105 44-45 -0.7690157991821849 1105 46-47 -0.8451638731997306 1105 48-49 -0.8431893013088256 1105 50-51 -0.88323415095104 1105 52-53 -0.7085773612485227 1105 54-55 -0.6551432963129642 1105 56-57 -0.6757109007052264 1105 58-59 -0.5810786826630618 1105 60-61 -0.6089594482593554 1105 62-63 -0.7126754184289368 1105 64-65 -0.6611497966502284 1105 66-67 -0.650800182095665 1105 68-69 -0.6664118445437524 1105 70-71 -0.6281235228981004 1105 72-73 -0.7269936828093932 1105 74-75 -0.7599368114456055 1105 76-77 -0.7483937143802635 1105 78-79 -0.6962752109190795 1105 80-81 -0.7389013368962019 1105 82-83 -0.800793990053954 1105 84-85 -0.7075211176021128 1105 86-87 -0.7348695875658819 1105 88-89 -0.7455810267344418 1105 90-91 -0.8044069383843322 1105 92-93 -0.8338743195842184 1105 94-95 -0.8014265427886542 1105 96-97 -0.8203217403877261 1105 98-99 -0.9739914843113446 1105 100 -0.9832092372795387 1106 1 -0.48432877436844635 1106 2 -0.5902110165874852 1106 3 -0.5416528773503977 1106 4 -0.4216002044065732 1106 5 -0.38540372327978645 1106 6 -0.3798294627437002 1106 7 -0.5406051872844131 1106 8 -0.47717110228563797 1106 9 -0.5344679197817044 1106 10-11 -0.5299934801688195 1106 12-13 -0.5495896100729709 1106 14-15 -0.6712820474130652 1106 16-17 -0.7351482077457305 1106 18-19 -0.8095103149859924 1106 20-21 -0.802793730176127 1106 22-23 -0.7632365015737648 1106 24-25 -0.7301748843811708 1106 26-27 -0.7023423096878361 1106 28-29 -0.871631820524577 1106 30-31 -0.974420003719338 1106 32-33 -0.8106989833578595 1106 34-35 -0.8872771610338006 1106 36-37 -0.8714565922559245 1106 38-39 -0.913114902424887 1106 40-41 -0.9614575950917015 1106 42-43 -0.9596296786618197 1106 44-45 -0.9291899925825504 1106 46-47 -0.9193901537516922 1106 48-49 -1.0350290599717837 1106 50-51 -1.0013939478504668 1106 52-53 -0.7743634340331269 1106 54-55 -0.8167407631200305 1106 56-57 -0.8476827568397951 1106 58-59 -0.8787918989733896 1106 60-61 -0.8614775053420018 1106 62-63 -0.7594195246750459 1106 64-65 -0.8595263975523508 1106 66-67 -0.9208752417162529 1106 68-69 -0.8517436357553052 1106 70-71 -0.8180088974044892 1106 72-73 -0.758308282830761 1106 74-75 -0.7230628490357418 1106 76-77 -0.7523483019089952 1106 78-79 -0.7200147054713852 1106 80-81 -0.8252556428723388 1106 82-83 -0.8043173189704866 1106 84-85 -0.7195073248963979 1106 86-87 -0.8196152849688758 1106 88-89 -0.8692340276404096 1106 90-91 -0.8606252136745312 1106 92-93 -0.9279226541840089 1106 94-95 -0.9767611606078184 1106 96-97 -0.950524635717322 1106 98-99 -0.9498584146958713 1106 100 -1.043525937512495 1107 1 -0.5397639698122028 1107 2 -0.5243080754662444 1107 3 -0.5852102056810047 1107 4 -0.4464768878748586 1107 5 -0.4007799000341876 1107 6 -0.43959201898045563 1107 7 -0.42957207551599197 1107 8 -0.46810666330240736 1107 9 -0.6524191298677664 1107 10-11 -0.6299635407382453 1107 12-13 -0.6377020963147615 1107 14-15 -0.8056630689942921 1107 16-17 -0.7698556478683756 1107 18-19 -0.7188778509511735 1107 20-21 -0.756651116114007 1107 22-23 -0.8286155026598792 1107 24-25 -0.8464847261398418 1107 26-27 -0.8523310956522607 1107 28-29 -0.8300530766419456 1107 30-31 -0.7773899285996819 1107 32-33 -0.8479987889711467 1107 34-35 -0.8518664395980196 1107 36-37 -0.8188891157152938 1107 38-39 -0.8950324782790133 1107 40-41 -0.9557381340680848 1107 42-43 -0.9081257182369882 1107 44-45 -0.9214025982791867 1107 46-47 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0.9731799957880867 2311 28-29 0.9907653580747535 2311 30-31 0.9606608229484479 2311 32-33 0.9770226493183003 2311 34-35 1.0666631047808366 2311 36-37 1.0808547454821849 2311 38-39 1.1443216790608588 2311 40-41 1.1577593431124313 2311 42-43 1.1594334706534042 2311 44-45 1.1689427415635407 2311 46-47 1.1625959949003501 2311 48-49 1.1487510362215971 2311 50-51 1.1370361469885566 2311 52-53 1.0461144558847124 2311 54-55 1.0109487619787814 2311 56-57 1.016853108415205 2311 58-59 0.9891534884708335 2311 60-61 1.0562069690411704 2311 62-63 1.0394692265792642 2311 64-65 0.9881760822744567 2311 66-67 1.0219509007513707 2311 68-69 0.9917389780044843 2311 70-71 1.0283010594901967 2311 72-73 0.9237781496900652 2311 74-75 0.8989350491117634 2311 76-77 0.9968137495185232 2311 78-79 1.080653095310982 2311 80-81 1.0291666626948945 2311 82-83 1.0045745731928548 2311 84-85 1.0987254009408964 2311 86-87 1.14438920513102 2311 88-89 1.1633942040795482 2311 90-91 1.140220579561742 2311 92-93 1.1591851392573957 2311 94-95 1.1212746755816028 2311 96-97 1.1700201963460017 2311 98-99 1.218149106137762 2311 100 1.3634899158606189 2312 1 0.49212106355609464 2312 2 0.4629565235792903 2312 3 0.4705514638311641 2312 4 0.41468777602197804 2312 5 0.3947512816225398 2312 6 0.4505870173488731 2312 7 0.4758855877616526 2312 8 0.4653430079855525 2312 9 0.5699545240553476 2312 10-11 0.602795656589997 2312 12-13 0.6081373180213845 2312 14-15 0.7768658271751292 2312 16-17 0.7802587209905312 2312 18-19 0.727966887445632 2312 20-21 0.6902872998877498 2312 22-23 0.7568871241500688 2312 24-25 0.814330328296542 2312 26-27 0.8297645526901221 2312 28-29 0.8078279161763078 2312 30-31 0.8305138727038042 2312 32-33 0.7865111415708341 2312 34-35 0.8089594243434419 2312 36-37 0.7916272928557078 2312 38-39 0.8633879257370509 2312 40-41 0.8613205993133377 2312 42-43 0.8836461179941111 2312 44-45 0.8350341541755313 2312 46-47 0.8841330847682727 2312 48-49 0.8684588587091753 2312 50-51 0.8307849464005486 2312 52-53 0.7554378036252913 2312 54-55 0.7164119970249345 2312 56-57 0.6431647432509209 2312 58-59 0.6998903102587377 2312 60-61 0.7340005193529606 2312 62-63 0.7071968054077544 2312 64-65 0.7432449029422585 2312 66-67 0.6943204865382953 2312 68-69 0.6943182006990725 2312 70-71 0.6261862809180414 2312 72-73 0.7384617797180155 2312 74-75 0.7150692599164259 2312 76-77 0.7532368104300673 2312 78-79 0.8013622700534064 2312 80-81 0.8290568445006024 2312 82-83 0.8076651931434071 2312 84-85 0.8040858155878787 2312 86-87 0.8667115960842509 2312 88-89 0.9522850086660029 2312 90-91 0.9493096220159174 2312 92-93 1.000650269232807 2312 94-95 0.9561313548677042 2312 96-97 0.9720344391609714 2312 98-99 1.0925979808566275 2312 100 1.1455518161924516 2313 1 0.4564010121034485 2313 2 0.4889547975915356 2313 3 0.5055911033911222 2313 4 0.437256613984907 2313 5 0.45048874765070934 2313 6 0.4082431175027921 2313 7 0.43852782338868224 2313 8 0.46490138576994156 2313 9 0.543448531638667 2313 10-11 0.5504656678380755 2313 12-13 0.5711656052282876 2313 14-15 0.7682899932542213 2313 16-17 0.7235064495739181 2313 18-19 0.693860780444183 2313 20-21 0.7239631164152769 2313 22-23 0.6779632289616231 2313 24-25 0.7236629320085299 2313 26-27 0.7521395908093069 2313 28-29 0.8080921318011534 2313 30-31 0.8601454413620999 2313 32-33 0.8745695650091747 2313 34-35 0.8952496901653504 2313 36-37 0.8878364125827858 2313 38-39 0.9100035144746954 2313 40-41 0.8836887696077866 2313 42-43 0.7946389289793245 2313 44-45 0.8442899546122149 2313 46-47 0.9561326092635838 2313 48-49 0.9151816761666822 2313 50-51 0.8727156435881582 2313 52-53 0.7879361394378179 2313 54-55 0.7168381438022706 2313 56-57 0.7928639120062186 2313 58-59 0.7771091109464408 2313 60-61 0.7487435268396041 2313 62-63 0.7883372456471633 2313 64-65 0.7712758828190474 2313 66-67 0.7705333048105558 2313 68-69 0.7437169666735315 2313 70-71 0.7506719523309542 2313 72-73 0.7757134654583595 2313 74-75 0.8005111898367403 2313 76-77 0.7990986466642767 2313 78-79 0.7893879847858223 2313 80-81 0.8094911830560179 2313 82-83 0.7728168514237197 2313 84-85 0.7980576898216469 2313 86-87 0.9060609168776992 2313 88-89 0.8712831144723587 2313 90-91 0.9489883304941316 2313 92-93 0.9016853826809061 2313 94-95 0.9948883751071875 2313 96-97 1.0904244679228512 2313 98-99 1.0984004189197485 2313 100 1.1216099883254635 2314 1 0.303415859938287 2314 2 0.2546297138432614 2314 3 0.3048806203496355 2314 4 0.23339013404439157 2314 5 0.19850606142510685 2314 6 0.22475492856917612 2314 7 0.2815468128242742 2314 8 0.2865109477789929 2314 9 0.3916041284166383 2314 10-11 0.4037571875113102 2314 12-13 0.4138164238248052 2314 14-15 0.44145876732235223 2314 16-17 0.5208177123824456 2314 18-19 0.48780890011533984 2314 20-21 0.5660997525268741 2314 22-23 0.6051505155066934 2314 24-25 0.59080922667885 2314 26-27 0.6031190873766832 2314 28-29 0.5587135592957395 2314 30-31 0.581011063207896 2314 32-33 0.61559768066072 2314 34-35 0.5998055373926263 2314 36-37 0.6399628831959703 2314 38-39 0.722006395480939 2314 40-41 0.7339125243482556 2314 42-43 0.6573222581409013 2314 44-45 0.7022744084168977 2314 46-47 0.5911065466392316 2314 48-49 0.5738177584146911 2314 50-51 0.6594679238181342 2314 52-53 0.6208348881203634 2314 54-55 0.588486496010411 2314 56-57 0.5805071572514251 2314 58-59 0.607708150961102 2314 60-61 0.3762641436211567 2314 62-63 0.5738635464366766 2314 64-65 0.6537030541669466 2314 66-67 0.6320563895064666 2314 68-69 0.6101747019291182 2314 70-71 0.5651869730728407 2314 72-73 0.6265959668922179 2314 74-75 0.6724021280575627 2314 76-77 0.6771858510173416 2314 78-79 0.7254150910461696 2314 80-81 0.7293367868207348 2314 82-83 0.6796902074322233 2314 84-85 0.7835998159262481 2314 86-87 0.782917025267551 2314 88-89 0.6149480907117173 2314 90-91 0.5807620343678366 2314 92-93 0.7640345501710435 2314 94-95 0.8330902705813834 2314 96-97 0.7698353716906183 2314 98-99 0.8880598939097588 2314 100 0.8472585154378969 2315 1 0.2695297225743296 2315 2 0.31276546810632055 2315 3 0.34884962807966957 2315 4 0.14597343981094468 2315 5 0.16387137015753694 2315 6 0.14044001190328714 2315 7 0.19073296430654096 2315 8 0.2541474239308954 2315 9 0.020929149465288788 2315 10-11 0.2105520242283987 2315 12-13 0.2237562561157489 2315 14-15 0.3755750743842938 2315 16-17 0.20558892359959913 2315 18-19 0.3773876024879428 2315 20-21 0.26790491261512983 2315 22-23 0.21519566833998738 2315 24-25 0.44625072791910014 2315 26-27 0.49774708275164414 2315 28-29 0.3810842685399294 2315 30-31 0.4210386789342522 2315 32-33 0.43883770947444845 2315 34-35 0.28949651640993324 2315 36-37 0.5007749463141025 2315 38-39 0.5179584720578347 2315 40-41 0.5872371805879908 2315 42-43 0.5556955993681214 2315 44-45 0.5306865569662733 2315 46-47 0.6059020475352739 2315 48-49 0.5623034123552983 2315 50-51 0.527308162242413 2315 52-53 0.2159716966193841 2315 54-55 0.39634638928834676 2315 56-57 0.49518257521013 2315 58-59 0.42697907929675694 2315 60-61 0.5153277723775815 2315 62-63 0.5483872724136418 2315 64-65 0.5366017728013688 2315 66-67 0.4315394609296632 2315 68-69 0.49158172305815384 2315 70-71 0.4521694390574993 2315 72-73 0.5398857034981148 2315 74-75 0.27546245240773004 2315 76-77 0.5207114845121623 2315 78-79 0.5589276728318957 2315 80-81 0.5817777604277374 2315 82-83 0.5423049315944262 2315 84-85 0.5987014626160203 2315 86-87 0.5886718602739549 2315 88-89 0.5541697904533649 2315 90-91 0.6933336693404399 2315 92-93 0.8123765631743147 2315 94-95 0.7999573889309666 2315 96-97 0.7763278627069425 2315 98-99 0.9641434227868579 2315 100 0.9274196084631399 2316 1 -0.07002338290627819 2316 2 -0.024884667985364928 2316 3 -0.06686375986018689 2316 4 -0.11807022417634983 2316 5 -0.13401188398359665 2316 6 -0.2538729267853981 2316 7 -0.18469938687369591 2316 8 -0.14472135905700867 2316 9 -0.12213255250878063 2316 10-11 -0.06908081484371564 2316 12-13 -0.11579236959178019 2316 14-15 -0.31937093479606204 2316 16-17 -0.11635510683738204 2316 18-19 -0.0035557825220138284 2316 20-21 0.02125762999028069 2316 22-23 -0.11524827504926805 2316 24-25 -0.1279639105356125 2316 26-27 -0.24522029982946947 2316 28-29 -0.18611624482932143 2316 30-31 -0.8360608803070306 2316 32-33 -0.5817138121622207 2316 34-35 -0.69176193889637 2316 36-37 -0.3807229008470827 2316 38-39 -0.35518364290460624 2316 40-41 -0.17552611754409497 2316 42-43 -0.11337911517904331 2316 44-45 -0.27215005233382783 2316 46-47 -0.3730535284021883 2316 48-49 -0.27549917402364343 2316 50-51 -0.13785272742779142 2316 52-53 -0.5173709147273939 2316 54-55 -0.3461728461354099 2316 56-57 -0.32852251559550893 2316 58-59 -0.6762635333238904 2316 60-61 -0.2276299712604981 2316 62-63 -0.24332059330356515 2316 64-65 -0.523375286938851 2316 66-67 -0.7903969369081594 2316 68-69 -0.842813560653731 2316 70-71 -0.5974189419822338 2316 72-73 -0.7695373824073712 2316 74-75 -0.5913048711507827 2316 76-77 -0.2905561320362473 2316 78-79 -0.3138443406880995 2316 80-81 -0.3054456507389993 2316 82-83 -0.15885325959634145 2316 84-85 -0.15110518528230799 2316 86-87 -0.09024743162466109 2316 88-89 -0.09543431225445786 2316 90-91 -0.6093556169956145 2316 92-93 -0.6557508810620654 2316 94-95 -0.2362770220956456 2316 96-97 0.07372728012412821 2316 98-99 0.10853476812004104 2316 100 -0.035865105532010944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 232.0 1 554.0 2 322.0 3 330.0 4 311.0 5 253.0 6 357.0 7 1010.0 8 2571.0 9 4077.0 10 6659.0 11 11243.0 12 17108.0 13 22230.0 14 26215.0 15 29755.0 16 34806.0 17 41621.0 18 50633.0 19 58616.0 20 69073.0 21 80252.0 22 89842.0 23 92369.0 24 94197.0 25 99015.0 26 107825.0 27 122023.0 28 141823.0 29 167289.0 30 201059.0 31 245506.0 32 306734.0 33 394391.0 34 536247.0 35 732427.0 36 1068442.0 37 1506874.0 38 1330166.0 39 295632.0 40 282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.91031814742729 17.891442711981867 12.190030709636456 24.00820843095439 2 16.366336173452623 19.649430879885717 40.07996033931088 23.904272607350784 3 19.48788018827728 25.18451463925454 29.170586606597915 26.15701856587026 4 12.875919626735596 15.676479147825598 37.13835629727218 34.309244928166635 5 13.485010187980063 37.06767427404227 34.16403111045352 15.283284427524144 6 31.709472456396544 35.585213068197824 18.17088652051151 14.534427954894127 7 28.270515710718197 31.91129803192665 21.75026246612356 18.067923791231596 8 26.61165267091184 34.95295478315782 19.98439546577106 18.450997080159283 9 27.1123773177965 15.760571761006423 20.098354831291118 37.028696089905964 10-11 25.644228708327578 25.56401623729771 27.888913427119448 20.902841627255263 12-13 25.81407834749997 23.288196532966417 28.100119854426982 22.79760526510664 14-15 23.22379114842527 24.731124979844544 25.889431430892078 26.155652440838107 16-17 22.714847491239336 27.470368993203053 26.650165698267646 23.164617817289958 18-19 22.439213770059858 26.71836705107059 28.653144016481125 22.189275162388423 20-21 23.32840204180636 26.070912560632443 28.501262371816626 22.09942302574457 22-23 23.173139201730066 25.884581044072135 28.321968881698645 22.620310872499154 24-25 22.67167481272049 25.864508806667086 28.66241499803951 22.80140138257291 26-27 22.687993260119274 26.06713108532065 28.352922864779888 22.891952789780184 28-29 22.541134296438642 26.506210329503222 28.39328403551371 22.559371338544423 30-31 23.207730565842883 26.152193777754185 28.39490328466285 22.245172371740086 32-33 22.948419249499953 26.255607327999947 28.232749756588266 22.56322366591183 34-35 23.03910999794913 26.35984012736924 28.29117532827052 22.30987454641112 36-37 22.991130185657568 26.295374063578887 28.131830214334492 22.581665536429053 38-39 22.893948571913654 26.46776410501172 28.151151606604962 22.487135716469663 40-41 23.01180972215335 26.05350124440718 28.324957563937087 22.609731469502382 42-43 22.78022676432059 26.292147972506484 28.033567425517532 22.89405783765539 44-45 22.603375361518864 26.570803851659807 28.145427005496792 22.68039378132454 46-47 22.607145100004015 26.28209747867254 27.80852214430264 23.302235277020813 48-49 22.765829757379894 26.38864235237472 27.810865325946317 23.034662564299072 50-51 22.706892172353488 26.61981699351643 27.700555858825144 22.972734975304938 52-53 22.68265016446971 26.82972003394712 27.438321198002196 23.04930860358098 54-55 22.500238999795716 26.619975674923214 27.731388896523384 23.148396428757685 56-57 22.74356850619272 26.624475091420543 27.42315694017562 23.208799462211122 58-59 22.590949205481046 26.6897364158445 27.697729437821366 23.02158494085309 60-61 22.872618152960587 26.70456494753557 27.56753413299403 22.85528276650981 62-63 22.818957545371273 26.667641282210052 27.559051371421813 22.954349800996862 64-65 22.61701836464354 26.42787316709493 27.567870219495948 23.38723824876558 66-67 22.662211891522478 26.45470172713108 27.41251260825286 23.470573773093584 68-69 22.36311924482485 26.56643195169059 27.582916596542688 23.487532206941875 70-71 22.53185337492377 26.29701307694523 27.606285398725987 23.56484814940501 72-73 22.566212101878047 26.650595629350725 27.314548072671425 23.468644196099795 74-75 22.68250840306462 26.6388855916053 27.266761428092757 23.41184457723732 76-77 22.606955500861975 26.512982462994184 27.225307501134445 23.6547545350094 78-79 22.525276659818026 26.492258684427796 27.37243831936494 23.610026336389236 80-81 22.623675551620387 26.640510227139885 27.1498182058161 23.585996015423625 82-83 22.94282627932378 26.503075450749524 27.341047034368444 23.213051235558254 84-85 23.045550115558118 26.606554614977306 26.956650663486357 23.39124460597822 86-87 22.626011368524125 26.746259274725976 27.248176046210375 23.37955331053952 88-89 22.744874815814452 26.352216052771187 27.26200328132507 23.64090585008929 90-91 23.112235326472323 26.279382183602156 27.189567072167332 23.41881541775819 92-93 23.097385874587754 26.345696165609578 27.106661662069726 23.450256297732942 94-95 22.89915812462 26.22360055290479 27.19468451919821 23.682556803277 96-97 22.912566801437915 26.16000133754727 27.189856382113714 23.7375754789011 98-99 22.90920695931515 26.2053350674674 26.998045091159774 23.887412882057674 100 23.16062507283369 26.154669313114077 26.76307654523332 23.92162906881891 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2417.0 1 1825.5 2 3314.0 3 4660.5 4 2948.5 5 2201.5 6 3082.0 7 5328.5 8 8335.5 9 9586.0 10 8271.0 11 6905.0 12 6942.5 13 7503.5 14 8148.0 15 8652.5 16 9280.0 17 10041.0 18 10714.5 19 11541.0 20 12634.5 21 13832.0 22 15604.5 23 18402.0 24 21977.5 25 26468.5 26 32185.5 27 39175.0 28 47518.5 29 58189.5 30 71229.0 31 84143.5 32 99309.5 33 116233.5 34 132769.5 35 149409.0 36 168399.5 37 189218.0 38 203682.5 39 212390.0 40 218219.5 41 223191.5 42 232005.5 43 245414.5 44 259903.5 45 276834.5 46 293327.0 47 306067.5 48 318477.5 49 328234.0 50 332281.5 51 332136.5 52 326203.0 53 316022.5 54 304519.0 55 284501.0 56 259689.0 57 231592.5 58 202428.5 59 177168.5 60 148173.0 61 118656.0 62 95727.5 63 76277.5 64 58027.0 65 43119.0 66 32260.0 67 25071.0 68 19418.0 69 14584.0 70 11251.0 71 8642.5 72 6838.5 73 5509.0 74 4327.5 75 3316.5 76 2382.5 77 1589.0 78 1049.0 79 727.5 80 500.0 81 351.0 82 270.0 83 195.0 84 133.5 85 107.5 86 82.5 87 62.0 88 50.5 89 45.0 90 38.0 91 24.5 92 18.0 93 19.5 94 15.0 95 12.5 96 9.5 97 2.5 98 2.5 99 5.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025017611823030497 2 0.1084305096722042 3 0.016307127666537635 4 0.017471028566758667 5 0.019335773019800957 6 0.044578655984809715 7 0.04537962004517688 8 0.03690692209410552 9 0.0320135322877999 10-11 0.03548020486157651 12-13 0.034597892888828315 14-15 0.030774541006919454 16-17 0.03705710285542436 18-19 0.035161070743773976 20-21 0.04529827213279584 22-23 0.0453545899182904 24-25 0.06400203444871333 26-27 0.0559798787815985 28-29 0.04853967356459419 30-31 0.046768792087376165 32-33 0.042419807540851354 34-35 0.04396541787609111 36-37 0.03895313496707475 38-39 0.0441656588911829 40-41 0.053051353935881077 42-43 0.05524774757016915 44-45 0.05780707804431108 46-47 0.05902729673002668 48-49 0.059302628125777884 50-51 0.05332042779991067 52-53 0.0627004678506167 54-55 0.049590938893826084 56-57 0.05428408768503991 58-59 0.05200008860664918 60-61 0.047769997162835115 62-63 0.054015013821010316 64-65 0.0526884170960272 66-67 0.05049202346173913 68-69 0.05650551144621445 70-71 0.05708120436460335 72-73 0.059872063512445166 74-75 0.05004773870950423 76-77 0.05079864251609844 78-79 0.0605979371921529 80-81 0.06044149889911244 82-83 0.05083618770642815 84-85 0.0570999769597682 86-87 0.053639561917713206 88-89 0.06104222194438781 90-91 0.06689301410410105 92-93 0.07148604238776898 94-95 0.0750215478104834 96-97 0.07016570319450749 98-99 0.07968340894308912 100 0.12341104061375874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7990371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.69316502372857 #Duplication Level Percentage of deduplicated Percentage of total 1 75.8454433851441 46.033000116724125 2 14.527398501506733 17.634275892348303 3 4.572683751568503 8.325919486058082 4 1.8496735368708197 4.490501648532973 5 0.9520800083272657 2.8892374530599803 6 0.5720354382469182 2.0831184751764655 7 0.3602997224657074 1.5307411359530347 8 0.23858111712265107 1.1584194490456459 9 0.17811877769765586 0.9729533131765784 >10 0.838903304560835 8.854347773060873 >50 0.04339408782172532 1.7929367375729934 >100 0.019731152286814362 2.126724342354323 >500 0.0011226454435541123 0.44712076119868766 >1k 4.276567495262188E-4 0.5151261225120677 >5k 2.6728546812931146E-5 0.12327402539366239 >10k+ 8.018564043879343E-5 1.022303267832322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27253 0.34107302401853434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21850 0.27345413623472553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13915 0.174147107812641 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.2515063443236866E-5 0.0 0.0 0.0 6 0.0 1.2515063443236866E-5 0.0 0.0 0.0 7 0.0 1.2515063443236866E-5 0.0 0.0 0.0 8 0.0 1.2515063443236866E-5 0.0 0.0 0.0 9 0.0 1.2515063443236866E-5 0.0 0.0 0.0 10-11 6.257531721618433E-6 1.2515063443236866E-5 0.0 6.257531721618433E-6 0.0 12-13 1.2515063443236866E-5 1.2515063443236866E-5 0.0 1.2515063443236866E-5 0.0 14-15 1.2515063443236866E-5 1.2515063443236866E-5 0.0 1.87725951648553E-5 2.5030126886473733E-5 16-17 1.2515063443236866E-5 1.2515063443236866E-5 0.0 2.5030126886473733E-5 3.75451903297106E-5 18-19 1.2515063443236866E-5 1.2515063443236866E-5 0.0 4.380272205132903E-5 6.883284893780277E-5 20-21 1.2515063443236866E-5 1.2515063443236866E-5 0.0 1.2515063443236865E-4 7.50903806594212E-5 22-23 1.2515063443236866E-5 1.2515063443236866E-5 0.0 4.505422839565272E-4 8.760544410265806E-5 24-25 1.2515063443236866E-5 1.2515063443236866E-5 0.0 0.0011326132416129365 1.0012050754589493E-4 26-27 1.2515063443236866E-5 1.2515063443236866E-5 0.0 0.002271484014947491 1.0637803926751336E-4 28-29 1.2515063443236866E-5 1.2515063443236866E-5 0.0 0.006413970014658894 1.126355709891318E-4 30-31 1.2515063443236866E-5 3.75451903297106E-5 0.0 0.021713635074015962 1.126355709891318E-4 32-33 1.2515063443236866E-5 3.75451903297106E-5 0.0 0.056674464802698145 1.3766569787560553E-4 34-35 1.2515063443236866E-5 3.75451903297106E-5 0.0 0.10799873998341253 1.501807613188424E-4 36-37 2.5030126886473733E-5 3.75451903297106E-5 0.0 0.1896970741408628 1.501807613188424E-4 38-39 3.75451903297106E-5 3.75451903297106E-5 0.0 0.3138527610294941 1.501807613188424E-4 40-41 3.75451903297106E-5 3.75451903297106E-5 0.0 0.45601136668122166 1.501807613188424E-4 42-43 3.75451903297106E-5 3.75451903297106E-5 0.0 0.5898975153969697 1.501807613188424E-4 44-45 3.75451903297106E-5 3.75451903297106E-5 0.0 0.7470303944585301 1.501807613188424E-4 46-47 3.75451903297106E-5 3.75451903297106E-5 0.0 0.9269907492405547 1.6269582476207927E-4 48-49 3.75451903297106E-5 3.75451903297106E-5 0.0 1.121287109196807 1.6269582476207927E-4 50-51 4.380272205132903E-5 3.75451903297106E-5 0.0 1.3254891418683814 1.689533564836977E-4 52-53 5.0060253772947465E-5 3.75451903297106E-5 0.0 1.527012950963103 2.0024101509178986E-4 54-55 5.0060253772947465E-5 3.75451903297106E-5 0.0 1.7554629190559488 2.3778620542150045E-4 56-57 6.257531721618433E-5 3.75451903297106E-5 0.0 2.011339648684648 2.503012688647373E-4 58-59 7.50903806594212E-5 4.380272205132903E-5 0.0 2.297183697728178 2.628163323079742E-4 60-61 7.50903806594212E-5 5.0060253772947465E-5 0.0 2.580743747693317 2.878464591944479E-4 62-63 7.50903806594212E-5 5.0060253772947465E-5 0.0 2.877707430606163 2.878464591944479E-4 64-65 7.50903806594212E-5 5.63177854945659E-5 0.0 3.1703471590993706 2.878464591944479E-4 66-67 7.50903806594212E-5 6.257531721618433E-5 0.0 3.5020714307258074 2.878464591944479E-4 68-69 7.50903806594212E-5 6.257531721618433E-5 0.0 3.8720104485761677 2.878464591944479E-4 70-71 7.50903806594212E-5 8.134791238103962E-5 0.0 4.260309064497756 3.0661905435930325E-4 72-73 7.50903806594212E-5 1.0012050754589493E-4 0.0 4.627200664399688 3.2539164952415854E-4 74-75 8.760544410265806E-5 1.0012050754589493E-4 0.0 4.991295147622056 3.2539164952415854E-4 76-77 8.760544410265806E-5 1.0012050754589493E-4 0.0 5.394812080690621 3.3164918124577695E-4 78-79 1.0012050754589493E-4 1.0012050754589493E-4 0.0 5.838846531656666 3.3790671296739537E-4 80-81 1.126355709891318E-4 1.0012050754589493E-4 0.0 6.284132488967034 3.5042177641063225E-4 82-83 1.126355709891318E-4 1.0012050754589493E-4 0.0 6.735250465841949 3.6293683985386913E-4 84-85 1.126355709891318E-4 1.126355709891318E-4 0.0 7.186650031644338 3.6293683985386913E-4 86-87 1.126355709891318E-4 1.126355709891318E-4 0.0 7.654362982644987 3.6293683985386913E-4 88 1.126355709891318E-4 1.126355709891318E-4 0.0 8.0211044017856 3.6293683985386913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11505 0.0 52.16416 1 GTATCAA 21345 0.0 41.69012 1 TACCTGG 6780 0.0 33.29645 2 ATCAACG 26325 0.0 32.951435 3 CAACGCA 26390 0.0 32.905678 5 TATCAAC 27430 0.0 31.994272 2 TCAACGC 27260 0.0 31.907211 4 AACGCAG 28090 0.0 31.28246 6 GTACATG 27985 0.0 30.117636 1 TACATGG 28750 0.0 28.578634 2 ACGCAGA 31800 0.0 27.515827 7 GTACCTG 8525 0.0 27.25466 1 ACATGGG 29470 0.0 27.059063 3 CGCAGAG 32740 0.0 26.738834 8 GTGGTAT 5335 0.0 25.654688 1 ACCTGGG 8795 0.0 25.004663 3 CATGGGG 21810 0.0 24.195377 4 GCAGAGT 36015 0.0 23.760405 9 GTACTAG 2270 0.0 23.206003 1 ATGGGGG 15080 0.0 22.965422 5 >>END_MODULE