FastQCFastQC Report
Mon 6 Jun 2016
ERR1211367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7990371
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243280.30446646344706646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205110.2566964662842314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137330.17186936626597188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119700.054.224611
GTATCAA217650.040.9697531
TACCTGG72800.034.7383272
ATCAACG272750.031.9298843
CAACGCA273550.031.8293325
TATCAAC282850.031.301652
TCAACGC281850.030.8991684
AACGCAG290050.030.325786
GTACATG284200.028.667781
GTACCTG91100.028.0767331
TACATGG288550.027.8214932
ACCTGGG89000.027.626473
ACGCAGA330950.026.492967
ACATGGG296300.026.2248383
GTGGTAT55700.025.6145531
CGCAGAG341350.025.5895888
CATGGGG214950.024.907784
ATGGGGG143900.023.733235
TGGTATC60100.022.7179972
GCAGAGT383500.022.4705549
Produced by FastQC (version 0.11.5)