##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211367_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7990371 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08630562961344 33.0 31.0 34.0 30.0 34.0 2 32.20618241631083 33.0 31.0 34.0 30.0 34.0 3 32.27648628580575 34.0 31.0 34.0 30.0 34.0 4 35.92196069494145 37.0 35.0 37.0 35.0 37.0 5 35.82677788052645 37.0 35.0 37.0 35.0 37.0 6 35.845399168574275 37.0 35.0 37.0 35.0 37.0 7 35.76089270448143 37.0 35.0 37.0 33.0 37.0 8 35.740690013016916 37.0 35.0 37.0 33.0 37.0 9 37.36069501653928 39.0 37.0 39.0 34.0 39.0 10-11 37.305883982107964 39.0 37.0 39.0 34.0 39.0 12-13 37.22974865622635 39.0 37.0 39.0 33.0 39.0 14-15 38.460683365015214 40.0 38.0 41.0 34.0 41.0 16-17 38.43894069499402 40.0 38.0 41.0 34.0 41.0 18-19 38.43844284577024 40.0 38.0 41.0 34.0 41.0 20-21 38.40356768165083 40.0 38.0 41.0 34.0 41.0 22-23 38.290710969490654 40.0 38.0 41.0 34.0 41.0 24-25 38.19866386679667 40.0 38.0 41.0 33.0 41.0 26-27 38.098417269986584 40.0 37.0 41.0 33.0 41.0 28-29 37.98332580552267 40.0 37.0 41.0 33.0 41.0 30-31 37.9837976859898 40.0 37.0 41.0 33.0 41.0 32-33 37.910221940883595 40.0 37.0 41.0 33.0 41.0 34-35 37.76861205818854 40.0 37.0 41.0 33.0 41.0 36-37 37.6119187081551 40.0 37.0 41.0 32.5 41.0 38-39 37.49262456023631 40.0 36.0 41.0 32.0 41.0 40-41 37.37867340577803 40.0 36.0 41.0 32.0 41.0 42-43 37.269903550160564 40.0 36.0 41.0 31.0 41.0 44-45 37.11175232038663 39.0 36.0 41.0 31.0 41.0 46-47 36.87299238045392 39.0 35.5 41.0 30.5 41.0 48-49 35.30381223850557 39.0 35.0 40.5 24.0 41.0 50-51 36.053101914792194 39.0 35.0 40.0 28.0 41.0 52-53 36.15559997151571 39.0 35.0 40.0 29.0 41.0 54-55 35.98478762750816 38.0 35.0 40.0 28.5 41.0 56-57 35.773289625725766 38.0 34.5 40.0 28.0 41.0 58-59 35.535729379774736 38.0 34.0 40.0 28.0 41.0 60-61 35.27189325752208 37.5 34.0 40.0 28.0 41.0 62-63 34.94108083341813 37.0 34.0 40.0 27.0 41.0 64-65 34.60441286143034 36.5 33.5 39.0 26.0 41.0 66-67 34.261740975481615 36.0 33.0 39.0 26.0 40.0 68-69 33.89567399310995 35.5 33.0 39.0 26.0 40.0 70-71 33.523691703426536 35.0 32.5 38.0 26.0 40.0 72-73 33.13881783461619 35.0 32.0 37.0 25.0 39.5 74-75 32.77478392430089 35.0 32.0 37.0 25.0 39.0 76-77 31.843075496744767 34.0 30.5 36.0 23.5 38.0 78-79 32.284793284316834 34.5 31.0 36.0 25.0 38.0 80-81 32.162321937241714 34.5 31.0 35.5 25.0 37.0 82-83 31.890177001793784 34.0 31.0 35.0 24.0 37.0 84-85 31.61295120088917 34.0 31.0 35.0 24.0 36.0 86-87 31.352883364239283 34.0 31.0 35.0 23.0 36.0 88-89 31.12984835122174 34.0 31.0 35.0 23.0 35.5 90-91 30.908002519532573 34.0 30.0 35.0 20.5 35.0 92-93 30.716978723516092 34.0 30.0 35.0 20.0 35.0 94-95 30.51698481835199 34.0 30.0 35.0 20.0 35.0 96-97 30.32667463125304 34.0 29.0 35.0 19.5 35.0 98-99 30.1023852960019 34.0 29.0 35.0 19.0 35.0 100 27.683964111303467 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3361051124707153 1101 2 -0.34010625174125053 1101 3 -0.3180061220293986 1101 4 -0.21684238339093298 1101 5 -0.24418582445678538 1101 6 -0.28955388869840704 1101 7 -0.30914369152897336 1101 8 -0.3147671183598675 1101 9 -0.40221700872283606 1101 10-11 -0.3439753297087478 1101 12-13 -0.45556437474036926 1101 14-15 -0.5813588933909131 1101 16-17 -0.5620001294878634 1101 18-19 -0.5273078479082614 1101 20-21 -0.645714556337623 1101 22-23 -0.7093899311213008 1101 24-25 -0.6345127905209509 1101 26-27 -0.675790739887745 1101 28-29 -0.6653290493837929 1101 30-31 -0.5808096193631656 1101 32-33 -0.5625946089803833 1101 34-35 -0.6306230585046322 1101 36-37 -0.6345108958460983 1101 38-39 -0.6344747467081078 1101 40-41 -0.6783824137230923 1101 42-43 -0.7297556302209003 1101 44-45 -0.6916168974159973 1101 46-47 -0.6916380633618289 1101 48-49 0.5895520343306231 1101 50-51 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1105 68-69 -0.7564057004648959 1105 70-71 -0.7969480177009416 1105 72-73 -0.8094868137353117 1105 74-75 -0.8432462571450614 1105 76-77 -0.8067596422011398 1105 78-79 -0.6469631347808971 1105 80-81 -0.6419287773181814 1105 82-83 -0.6091032306578548 1105 84-85 -0.606775727820839 1105 86-87 -0.6234052331079702 1105 88-89 -0.7361682467891697 1105 90-91 -0.6900678856049858 1105 92-93 -0.7348365075808942 1105 94-95 -0.7594524006966061 1105 96-97 -0.6856636072949449 1105 98-99 -0.7778452255140529 1105 100 -0.8280635471999531 1106 1 -0.372830689608989 1106 2 -0.31163865169208904 1106 3 -0.3697587206878623 1106 4 -0.19495891389888698 1106 5 -0.26764385135216884 1106 6 -0.36965215906238313 1106 7 -0.3476269611490821 1106 8 -0.40414633491366203 1106 9 -0.5224422365703631 1106 10-11 -0.5350092303161205 1106 12-13 -0.49150109651735363 1106 14-15 -0.6308019282747068 1106 16-17 -0.6833969367117163 1106 18-19 -0.6460970218356508 1106 20-21 -0.6987705673726197 1106 22-23 -0.7710134296562146 1106 24-25 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2208 54-55 1.089162850150096 2208 56-57 1.1150077627560009 2208 58-59 1.0893728350354976 2208 60-61 1.1446704682832731 2208 62-63 1.0910016005980268 2208 64-65 1.0795513124917306 2208 66-67 1.067048299760522 2208 68-69 1.066347663395213 2208 70-71 1.15003932432748 2208 72-73 1.134593075780252 2208 74-75 1.151243603534077 2208 76-77 1.1309694735471219 2208 78-79 0.9989222568990002 2208 80-81 1.030915881740924 2208 82-83 1.002355985682616 2208 84-85 0.9876792675339914 2208 86-87 1.0600308910462068 2208 88-89 1.0968798948080298 2208 90-91 1.0387562974058824 2208 92-93 1.0110004991330541 2208 94-95 1.1068264956945129 2208 96-97 1.0414449491502182 2208 98-99 1.1614377142758627 2208 100 1.423243944259191 2209 1 0.36348000713164197 2209 2 0.38937821548459794 2209 3 0.4203082613194695 2209 4 0.25777721897004824 2209 5 0.37211380275663686 2209 6 0.407780531929113 2209 7 0.4622949540131245 2209 8 0.48839861634034776 2209 9 0.6162632646539876 2209 10-11 0.6328289573369261 2209 12-13 0.6618530685650086 2209 14-15 0.8114175123251499 2209 16-17 0.8161586154639835 2209 18-19 0.7706823664440208 2209 20-21 0.8386494542246652 2209 22-23 0.7953679905935189 2209 24-25 0.8349696574442618 2209 26-27 0.9032540721656872 2209 28-29 0.9104428518277672 2209 30-31 0.8942834996178846 2209 32-33 0.879565999904365 2209 34-35 0.945256407167939 2209 36-37 0.951363599338741 2209 38-39 0.9398243368582229 2209 40-41 1.0146472538755376 2209 42-43 1.017921210412844 2209 44-45 1.0214397195457963 2209 46-47 1.0608285411737413 2209 48-49 2.3310779740634615 2209 50-51 1.484635708363136 2209 52-53 1.1651822323387293 2209 54-55 1.0984398149711225 2209 56-57 1.1497352412208741 2209 58-59 1.1682377946904694 2209 60-61 1.1655075951953648 2209 62-63 1.1186756411444847 2209 64-65 1.1238137388812888 2209 66-67 1.147554763260409 2209 68-69 1.2530927609697713 2209 70-71 1.1984293974343387 2209 72-73 1.1950116214631592 2209 74-75 1.2225588754039336 2209 76-77 1.15929606098506 2209 78-79 1.097691300822369 2209 80-81 1.0744885205787966 2209 82-83 1.0242374388148399 2209 84-85 1.1111719272256977 2209 86-87 1.0697309514748916 2209 88-89 1.0953756707242412 2209 90-91 1.1160923875512623 2209 92-93 1.086588245142984 2209 94-95 1.0776662056368096 2209 96-97 1.1736503237488414 2209 98-99 1.1492321974920898 2209 100 1.4771710980679735 2210 1 0.5550650240899486 2210 2 0.49234701278045634 2210 3 0.5110799804405701 2210 4 0.32208734484345314 2210 5 0.38069614601797497 2210 6 0.43961534283182147 2210 7 0.5060579800292544 2210 8 0.48025379443843264 2210 9 0.6110187865131138 2210 10-11 0.6280446652348104 2210 12-13 0.6469471534053426 2210 14-15 0.8013971853357447 2210 16-17 0.8003470321241863 2210 18-19 0.7741527044900707 2210 20-21 0.8226495690988571 2210 22-23 0.8182659622023678 2210 24-25 0.8267942273121847 2210 26-27 0.8824205105198857 2210 28-29 0.9172052144454028 2210 30-31 0.9492760241794187 2210 32-33 0.899400983118376 2210 34-35 0.9367846705285316 2210 36-37 0.9329648020716022 2210 38-39 0.9354443272662252 2210 40-41 0.9878933402551908 2210 42-43 0.9401327569928881 2210 44-45 0.9770833625512481 2210 46-47 1.0400385513113903 2210 48-49 2.3122303747906088 2210 50-51 1.5007639883804913 2210 52-53 1.2174663564373347 2210 54-55 1.1912761508288057 2210 56-57 1.1897276308052298 2210 58-59 1.1197216936356327 2210 60-61 1.0602508197715466 2210 62-63 1.1818579263943505 2210 64-65 1.159844617065822 2210 66-67 1.19642749126416 2210 68-69 1.1756174201523848 2210 70-71 1.1058707699512667 2210 72-73 1.2057523188069794 2210 74-75 1.1633648614984153 2210 76-77 1.083320494727058 2210 78-79 0.9904254618243016 2210 80-81 0.9497131714330322 2210 82-83 1.0051366625524345 2210 84-85 1.0337812946421963 2210 86-87 1.0783973883931672 2210 88-89 1.1405346192807038 2210 90-91 1.176843595884236 2210 92-93 1.1988341965454552 2210 94-95 1.1504837997690345 2210 96-97 1.2153572050005117 2210 98-99 1.2637001834889254 2210 100 1.4764079313309466 2211 1 0.5267369783886195 2211 2 0.4711262116010104 2211 3 0.46227288007356293 2211 4 0.32636585271657736 2211 5 0.38285267696919334 2211 6 0.4366355072051391 2211 7 0.4490283189570121 2211 8 0.4753385337013185 2211 9 0.600555007524683 2211 10-11 0.5966326557722041 2211 12-13 0.5598342151251003 2211 14-15 0.7211979657108714 2211 16-17 0.723389349957877 2211 18-19 0.7431383826105602 2211 20-21 0.7477088369156277 2211 22-23 0.6988661616969551 2211 24-25 0.7214838821953293 2211 26-27 0.8562777575051612 2211 28-29 0.863136331021245 2211 30-31 0.8232585611455718 2211 32-33 0.8293316866437337 2211 34-35 0.8995659196879799 2211 36-37 0.8799069735400096 2211 38-39 0.9317455814110502 2211 40-41 0.9202081719364585 2211 42-43 0.8591917742097195 2211 44-45 0.9307340589980271 2211 46-47 1.0573597904681478 2211 48-49 2.2724478514596456 2211 50-51 1.4253297294518958 2211 52-53 1.1394511478708083 2211 54-55 1.1881818841079266 2211 56-57 1.089048840534474 2211 58-59 1.039567763757283 2211 60-61 1.0635823541648577 2211 62-63 1.1040916699552525 2211 64-65 1.1351053801466193 2211 66-67 1.1753444401553494 2211 68-69 1.189708523348692 2211 70-71 1.1248431457691481 2211 72-73 1.1934629186249808 2211 74-75 1.1933421963188309 2211 76-77 0.9041097167460812 2211 78-79 0.8174412500196837 2211 80-81 0.8796068908287467 2211 82-83 0.921702430121929 2211 84-85 1.0166934449840497 2211 86-87 1.0703841159175305 2211 88-89 1.0236792336856944 2211 90-91 1.071470517560975 2211 92-93 1.0817174420090225 2211 94-95 1.1131251683589305 2211 96-97 1.1264886357450479 2211 98-99 1.1873902728606929 2211 100 1.4852519985172954 2212 1 0.557880828516673 2212 2 0.5171893862093597 2212 3 0.507152195374303 2212 4 0.32973270124080045 2212 5 0.41251552253153534 2212 6 0.3065012135894065 2212 7 0.259979965131393 2212 8 0.35572644215315563 2212 9 0.533431193809534 2212 10-11 0.5612311681572137 2212 12-13 0.5902576788116747 2212 14-15 0.7611185158991773 2212 16-17 0.7147081678035576 2212 18-19 0.7770978522302059 2212 20-21 0.7806641800064966 2212 22-23 0.8006823796186566 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0.4262685847558174 2311 8 0.24664111411124168 2311 9 0.5004859123963783 2311 10-11 0.38133704790450196 2311 12-13 0.4822659140768266 2311 14-15 0.4849220801704419 2311 16-17 0.5036590028172654 2311 18-19 0.6337137201341037 2311 20-21 0.6935301347970722 2311 22-23 0.7530866405577115 2311 24-25 0.6874658496884294 2311 26-27 0.6946704988682129 2311 28-29 0.7768150033194416 2311 30-31 0.6651872459818691 2311 32-33 0.6365803100500997 2311 34-35 0.7551235084498629 2311 36-37 0.809174103190351 2311 38-39 0.8173015707172837 2311 40-41 0.7618736582138936 2311 42-43 0.7676317040324747 2311 44-45 0.846077639239418 2311 46-47 0.8708620143440484 2311 48-49 -5.98018587929775 2311 50-51 -1.0130954246086574 2311 52-53 0.4532017900251404 2311 54-55 0.8512785736865638 2311 56-57 0.978097833125922 2311 58-59 0.9944782168324551 2311 60-61 0.9123157456930784 2311 62-63 0.7671171069477722 2311 64-65 0.8447634998916271 2311 66-67 0.9727668428359308 2311 68-69 0.9641563892275613 2311 70-71 0.9742167770259442 2311 72-73 0.5532852536675676 2311 74-75 0.843817554309986 2311 76-77 0.926366413111122 2311 78-79 0.8196513961258844 2311 80-81 0.8656005899223445 2311 82-83 0.8843456638597864 2311 84-85 0.9257986794511481 2311 86-87 0.9256753772419266 2311 88-89 1.003291680839272 2311 90-91 1.033744938966933 2311 92-93 1.040135582637319 2311 94-95 1.0764787705002163 2311 96-97 1.1313608758368936 2311 98-99 1.165594380118197 2311 100 1.3271782604493936 2312 1 0.0011067362229439937 2312 2 0.1755437826265407 2312 3 0.16513396891683385 2312 4 -0.07619766930405092 2312 5 0.13717530520396792 2312 6 0.07339619483611415 2312 7 0.24610938261495363 2312 8 0.31089531776618173 2312 9 0.24945160259823496 2312 10-11 0.21263998095896852 2312 12-13 0.2532750732468543 2312 14-15 0.26129280494059515 2312 16-17 0.4048630206565704 2312 18-19 0.22988049370514574 2312 20-21 0.2782846957654215 2312 22-23 0.5701898675886312 2312 24-25 0.6634025154991647 2312 26-27 0.6129808225381055 2312 28-29 0.6999010344198098 2312 30-31 0.7382873302201318 2312 32-33 0.7605534440414132 2312 34-35 0.3889835831628332 2312 36-37 0.6332363382193549 2312 38-39 0.733493202574337 2312 40-41 0.5635831976933687 2312 42-43 0.5527928852372668 2312 44-45 0.7319402515738815 2312 46-47 0.8125225186157436 2312 48-49 -4.799282102548588 2312 50-51 -0.8051897864229787 2312 52-53 0.5150728473632071 2312 54-55 0.801479610672331 2312 56-57 0.8743915510740337 2312 58-59 0.9495163645989564 2312 60-61 0.9020426026437605 2312 62-63 0.6831250984758341 2312 64-65 0.9229046092792501 2312 66-67 0.8700025181018631 2312 68-69 0.7786956552025899 2312 70-71 0.8401757264405134 2312 72-73 0.9538756350830795 2312 74-75 0.6308606417371934 2312 76-77 0.828838942449206 2312 78-79 0.4688847462362915 2312 80-81 0.6284255284528157 2312 82-83 0.6885983666718296 2312 84-85 0.7699886967511205 2312 86-87 0.8621863675208594 2312 88-89 0.8415735851741211 2312 90-91 0.6288019002135314 2312 92-93 0.7544499178393025 2312 94-95 0.8975580975287016 2312 96-97 1.0183291915930646 2312 98-99 1.111219428817904 2312 100 0.6553622025582904 2313 1 0.06308663887730148 2313 2 0.23417529655421987 2313 3 0.27863335890831564 2313 4 0.09131115812272839 2313 5 0.17069484517448785 2313 6 0.2657735610926437 2313 7 0.19631906268256216 2313 8 0.2570114427940169 2313 9 0.42526237969570246 2313 10-11 0.25408540335589436 2313 12-13 0.07160121917402762 2313 14-15 0.18926429711990522 2313 16-17 0.18691241090400723 2313 18-19 0.43025532967958924 2313 20-21 0.38452115532069797 2313 22-23 0.5089874069792799 2313 24-25 0.3470454316395646 2313 26-27 0.4744400480111963 2313 28-29 0.458420576081636 2313 30-31 0.5799577547779862 2313 32-33 0.4645994937139548 2313 34-35 0.42417147122001353 2313 36-37 -0.28689360562503907 2313 38-39 0.17221166219351858 2313 40-41 0.41348764348629885 2313 42-43 0.5196538155410124 2313 44-45 0.5358636861636796 2313 46-47 0.39983418027686213 2313 48-49 -5.3507993460881025 2313 50-51 -1.3198138275349507 2313 52-53 0.12497810768848439 2313 54-55 0.23966184212702046 2313 56-57 0.28441417892725696 2313 58-59 0.3630383583143839 2313 60-61 0.7083573842659874 2313 62-63 0.7506490923958893 2313 64-65 0.8451285405962281 2313 66-67 0.8695740204109583 2313 68-69 0.8818844005483157 2313 70-71 0.8571692798408606 2313 72-73 0.6206377852978662 2313 74-75 0.8013800479593414 2313 76-77 0.7987210729420262 2313 78-79 0.735352799981797 2313 80-81 -0.19615706937907262 2313 82-83 0.20765375584183587 2313 84-85 0.6071886984739976 2313 86-87 0.8262942145366203 2313 88-89 0.78960440809783 2313 90-91 0.8264665387502532 2313 92-93 0.8638386367871433 2313 94-95 0.7723966427603841 2313 96-97 0.7545474709755418 2313 98-99 0.959951986476792 2313 100 1.034781939809367 2314 1 0.10577935349198242 2314 2 0.17933959236338382 2314 3 0.24690427537672832 2314 4 0.07850350306863874 2314 5 0.19295050731379604 2314 6 0.32353031729262227 2314 7 0.11982087637767336 2314 8 0.27070130530631786 2314 9 0.4822131713586657 2314 10-11 0.3799230768694173 2314 12-13 0.2729701627875656 2314 14-15 0.3052517442699241 2314 16-17 0.5333320646437798 2314 18-19 0.5632888089729491 2314 20-21 0.6730067810559461 2314 22-23 0.4206617163396018 2314 24-25 0.5341650052698483 2314 26-27 0.5877923232503193 2314 28-29 0.7040166581626863 2314 30-31 0.5921446555641694 2314 32-33 0.5505660914826009 2314 34-35 0.35917122796077905 2314 36-37 0.44454714501911496 2314 38-39 0.47064298905552704 2314 40-41 0.24422215914929524 2314 42-43 0.29427102782872794 2314 44-45 0.5422709751978871 2314 46-47 0.711001452331395 2314 48-49 -6.317279524477016 2314 50-51 -1.5711199265602218 2314 52-53 -0.8307562957700654 2314 54-55 -0.3879303442605462 2314 56-57 -0.0939043068405283 2314 58-59 0.41871461967300405 2314 60-61 0.6398760373350925 2314 62-63 0.5057103264015339 2314 64-65 0.702868697270425 2314 66-67 0.7251146725979609 2314 68-69 0.7704064164295517 2314 70-71 0.6160079887321217 2314 72-73 0.7636688097823594 2314 74-75 0.7608354874670766 2314 76-77 0.7697219684681436 2314 78-79 0.5170032506374937 2314 80-81 0.5511672641253966 2314 82-83 0.5721534730193092 2314 84-85 0.6376327718059756 2314 86-87 0.6644788136954354 2314 88-89 0.5616484021596122 2314 90-91 0.7769060143928463 2314 92-93 0.8518305954984235 2314 94-95 0.8418699443183115 2314 96-97 0.8400606056953386 2314 98-99 0.8782333536673228 2314 100 -0.5158688414903878 2315 1 0.12427781954259842 2315 2 0.19910110625858124 2315 3 0.28941829836381316 2315 4 0.11541039228935546 2315 5 0.22352644167292368 2315 6 0.28673292227566805 2315 7 0.34054238392478453 2315 8 0.3099829305815973 2315 9 0.4527806141202433 2315 10-11 0.2189647939034174 2315 12-13 0.4167377269460175 2315 14-15 0.3299285817887778 2315 16-17 0.43582966607663565 2315 18-19 0.5523111777370602 2315 20-21 0.4630342550839188 2315 22-23 0.4761389513352796 2315 24-25 0.5263938151786363 2315 26-27 0.5789546596144532 2315 28-29 0.664774321984055 2315 30-31 0.656314055721424 2315 32-33 0.7545915363897606 2315 34-35 0.7595222277330294 2315 36-37 0.6388782307584862 2315 38-39 0.7111423702200526 2315 40-41 0.7739258755629166 2315 42-43 0.7947026926249734 2315 44-45 0.8561837080598593 2315 46-47 0.7366637376841183 2315 48-49 -4.691560405191712 2315 50-51 -0.7289199458312012 2315 52-53 0.46831771818954593 2315 54-55 0.3714220709497269 2315 56-57 0.7012099458525185 2315 58-59 0.8659482679374335 2315 60-61 0.7370467328731891 2315 62-63 0.5520172930153322 2315 64-65 0.7465255821113814 2315 66-67 -0.057556852004488235 2315 68-69 0.7346132120778108 2315 70-71 0.6687405260812369 2315 72-73 0.7285944670825018 2315 74-75 0.9066038327700525 2315 76-77 0.8679201704960313 2315 78-79 0.7871896020432914 2315 80-81 0.7074916879797755 2315 82-83 0.6977647727180845 2315 84-85 0.7051553997493087 2315 86-87 0.7351280164230509 2315 88-89 0.7294049226038517 2315 90-91 0.7919445280128912 2315 92-93 0.8629626779699713 2315 94-95 0.5833209589302513 2315 96-97 0.6366691966363298 2315 98-99 0.587912563989974 2315 100 0.6355418045408712 2316 1 0.21145566865704524 2316 2 0.24712775560062994 2316 3 0.18523934947568677 2316 4 -0.015524606415546316 2316 5 0.15843784407544348 2316 6 0.13123942537753663 2316 7 0.23232919768087612 2316 8 0.3182296620457379 2316 9 0.4624095785071134 2316 10-11 0.43210248217065583 2316 12-13 0.3686587852764873 2316 14-15 0.49749250878758033 2316 16-17 0.4445916139074555 2316 18-19 0.18314394844487225 2316 20-21 0.4690354737520437 2316 22-23 0.580390489532256 2316 24-25 0.633436899555484 2316 26-27 0.6391502091403609 2316 28-29 0.7025755814159922 2316 30-31 0.6806910275646771 2316 32-33 0.5699494723820848 2316 34-35 0.5391013201437431 2316 36-37 0.47895663740298744 2316 38-39 0.5277697167931308 2316 40-41 0.1870046898455371 2316 42-43 0.13505096844311737 2316 44-45 0.38613563011605123 2316 46-47 0.445324083836816 2316 48-49 -4.788235171542883 2316 50-51 -0.9154208927405776 2316 52-53 0.37639179792525823 2316 54-55 0.6593655260224764 2316 56-57 0.5591876255423713 2316 58-59 0.35299568346929533 2316 60-61 0.5627347071240436 2316 62-63 0.46621688919882587 2316 64-65 0.4912134841763205 2316 66-67 0.5378541830990997 2316 68-69 0.22948076153578256 2316 70-71 -0.13363965591219795 2316 72-73 -0.051991916105826874 2316 74-75 0.1890312116144841 2316 76-77 0.23527257152701608 2316 78-79 0.3169305819883519 2316 80-81 0.2505759692840499 2316 82-83 0.2085272364658941 2316 84-85 0.29724947073778907 2316 86-87 0.35864477629447933 2316 88-89 0.24888239210530472 2316 90-91 0.22364523883186394 2316 92-93 0.3371947259693613 2316 94-95 0.6162704224190385 2316 96-97 0.6442740679118515 2316 98-99 0.6658183019211279 2316 100 -0.14879465826427563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 7.0 11 10.0 12 36.0 13 168.0 14 757.0 15 2303.0 16 5288.0 17 11092.0 18 18965.0 19 27120.0 20 36302.0 21 48548.0 22 60217.0 23 65805.0 24 69186.0 25 76118.0 26 90198.0 27 110086.0 28 135151.0 29 165147.0 30 203392.0 31 250896.0 32 314777.0 33 408997.0 34 563715.0 35 790730.0 36 1214861.0 37 1777759.0 38 1375350.0 39 167375.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8970483022051 18.682159780339934 13.049195547459503 24.371596369995466 2 16.085074929660948 20.052696875820924 39.72225069450467 24.139977500013455 3 19.12626497694786 25.091300607994903 29.8164050869779 25.966029328079337 4 12.861163645067075 16.07614160584161 37.04292328807318 34.01977146101813 5 13.32978904873629 37.20018287106826 34.280265556024034 15.189762524171407 6 31.891684547576986 35.91553076736795 17.78446917005669 14.408315514998376 7 28.251736559350388 31.98068825106826 21.590454708662392 18.177120480918965 8 26.66154614607401 35.403043251294456 19.66933218257205 18.266078420059493 9 26.868676855666184 15.797117176248063 19.94487331463652 37.38933265344923 10-11 25.681104146607137 25.750444649652117 27.701540352875014 20.86691085086573 12-13 25.7243583818986 23.330224797629697 28.093330410646473 22.852086409825233 14-15 23.06603832618305 24.847572574952817 25.817616477855815 26.268772621008313 16-17 22.660950350763976 27.576451803798946 26.62562922036716 23.13696862506992 18-19 22.381233761313073 26.782856940113103 28.64044915063701 22.195460147936817 20-21 23.207142850438355 26.112685054848857 28.511391997927504 22.168780096785284 22-23 23.04296818926523 25.89184107385493 28.367363744755707 22.697826992124128 24-25 22.59543131151352 25.869742536873304 28.7009702620061 22.83385588960708 26-27 22.63260673119396 26.08333123184415 28.408537757127633 22.87552427983426 28-29 22.479088063479026 26.543995320321795 28.437561676399103 22.53935493980008 30-31 23.125303772286255 26.15962557419885 28.455776418340843 22.25929423517405 32-33 22.911556637573423 26.242725072935453 28.277501787010838 22.568216502480286 34-35 22.971146451274368 26.368199060295687 28.351896181900475 22.308758306529462 36-37 22.915509297797655 26.30961790116147 28.1968604019745 22.578012399066377 38-39 22.839194119546434 26.431247573526797 28.240183153624365 22.48937515330241 40-41 22.93071925053359 26.06236955185695 28.433000256758305 22.57391094085116 42-43 22.74478701206882 26.267546761587322 28.13150209609634 22.85616413024752 44-45 22.549715666856642 26.541243716331092 28.237561312844434 22.671479303967836 46-47 22.51235973845005 26.28233186043243 27.90341987288485 23.301888528232674 48-49 22.78937538398422 26.464372789774494 27.803979612742452 22.942272213498835 50-51 22.666803271686042 26.57347734272862 27.75093208204137 23.00878730354397 52-53 22.629877510229175 26.783703462999718 27.485244548944838 23.101174477826266 54-55 22.452769449629635 26.571579338137724 27.75384817184458 23.22180304038806 56-57 22.687623458615374 26.5562192165046 27.451182120642358 23.304975204237667 58-59 22.439056626822094 26.662475443420234 27.767289984920605 23.131177944837063 60-61 22.777005627691267 26.7176035409461 27.61277754380344 22.89261328755919 62-63 22.769074742857228 26.665267795138377 27.51665613549621 23.049001326508193 64-65 22.484949011396395 26.431940591154362 27.55187734295475 23.531233054494493 66-67 22.475681538474827 26.470521591769327 27.444868702278036 23.608928167477814 68-69 22.237560397979134 26.543307089571343 27.59119019806694 23.627942314382583 70-71 22.367924847756356 26.32254477803032 27.600187750418133 23.709342623795195 72-73 22.363679004048343 26.660387286577386 27.316292299989968 23.659641409384307 74-75 22.46653678583299 26.692282366214258 27.196629781986815 23.64455106596593 76-77 22.38131992977287 26.598464768872006 27.177263413871227 23.842951887483892 78-79 22.250374435140035 26.581518721318936 27.314211926440557 23.853894917100472 80-81 22.267839416823023 26.800649723794802 27.091156884191808 23.84035397519037 82-83 22.551218091076844 26.647714098051225 27.252971730423752 23.54809608044818 84-85 22.64523542920162 26.743936123137086 26.910143900697193 23.7006845469641 86-87 22.229413770519386 26.86038907715849 27.16274079481818 23.747456357503946 88-89 22.231335441385134 26.564050413969525 27.208140027787426 23.996474116857915 90-91 22.533109543531015 26.47407825495527 27.144615240951758 23.84819696056196 92-93 22.450595903229832 26.58315750013221 27.057082607235827 23.90916398940213 94-95 22.138106472587094 26.452930906087097 27.194947339436908 24.214015281888894 96-97 22.107303060639964 26.408747067137533 27.24849660096462 24.235453271257878 98-99 22.045523844142267 26.488140388718072 27.07666489926565 24.38967086787401 100 21.999084586372504 26.47875231325975 27.13425206875718 24.387911031610564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2294.0 1 1765.5 2 2896.0 3 4070.5 4 2696.0 5 1955.5 6 2785.5 7 4945.0 8 7412.5 9 8566.0 10 7547.5 11 6182.0 12 6253.0 13 6814.0 14 7592.0 15 8430.5 16 9150.5 17 9956.5 18 10728.5 19 11551.0 20 12748.5 21 13992.0 22 15827.0 23 18619.0 24 22171.5 25 26566.5 26 32287.0 27 39778.0 28 48178.0 29 58672.0 30 72144.0 31 85404.5 32 100608.0 33 117205.0 34 132947.0 35 149269.0 36 169023.0 37 190563.5 38 204832.5 39 213149.5 40 218882.0 41 224933.5 42 234545.5 43 247594.0 44 261762.0 45 279868.0 46 296947.5 47 310402.0 48 323868.5 49 332840.5 50 336685.0 51 335284.0 52 328703.5 53 319269.0 54 307490.0 55 285796.0 56 257516.5 57 229003.5 58 200613.5 59 173606.0 60 143644.0 61 114599.0 62 91833.0 63 71952.5 64 54142.0 65 40115.0 66 29640.5 67 22732.0 68 17542.5 69 12844.0 70 9711.0 71 7583.5 72 6008.0 73 4805.0 74 3734.5 75 2706.0 76 1825.5 77 1129.5 78 710.5 79 400.0 80 242.5 81 164.5 82 100.5 83 58.0 84 29.0 85 19.0 86 14.5 87 12.0 88 11.5 89 8.5 90 3.5 91 2.0 92 2.5 93 2.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029936031756222583 2 0.0018021691358261088 3 1.0012050754589493E-4 4 0.02361592471738797 5 5.256326646159484E-4 6 7.50903806594212E-5 7 2.5030126886473733E-5 8 0.0037294889060845863 9 0.009686659105065335 10-11 0.0011451283050561733 12-13 8.134791238103964E-5 14-15 1.3766569787560553E-4 16-17 3.3164918124577695E-4 18-19 6.257531721618433E-6 20-21 4.380272205132903E-5 22-23 2.252711419782636E-4 24-25 4.380272205132903E-5 26-27 6.883284893780277E-5 28-29 9.761749485724756E-4 30-31 0.0027095112354607815 32-33 0.009661628978178861 34-35 0.018459718578774376 36-37 0.021338183170718856 38-39 0.020868868291597474 40-41 0.004586770751946311 42-43 0.0049997678455731285 44-45 0.00890446763986303 46-47 0.01153888849466439 48-49 1.2238981143679062 50-51 0.017539861415696467 52-53 0.018259477563682586 54-55 0.009173541503892622 56-57 0.007490265470777264 58-59 0.008247426809093095 60-61 0.005206266392386537 62-63 0.007477750407334027 64-65 0.019110501877822695 66-67 0.02883470617321774 68-69 0.028959856807650106 70-71 0.02636298114317846 72-73 0.02240822109511561 74-75 0.009254889416273662 76-77 0.014398580491444015 78-79 0.008378834975247083 80-81 0.0025843606010284128 82-83 0.014123249095692804 84-85 0.02347825901951236 86-87 0.022865020910793753 88-89 0.0323514390007673 90-91 0.02033072056353829 92-93 0.012871742751369118 94-95 0.006520348053926406 96-97 0.003879669667403428 98-99 0.015506163606170478 100 0.03410354788282046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7990371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.288640078593026 #Duplication Level Percentage of deduplicated Percentage of total 1 75.268394484053 45.37829144342628 2 14.870647505848572 17.930622304314632 3 4.661573316788126 8.431197476874374 4 1.9095664056419819 4.605006469436881 5 0.9861735321603035 2.972753056772366 6 0.5865926584065527 2.121892419325064 7 0.36091700856531006 1.5231436939345517 8 0.2517330491386054 1.2141314556323324 9 0.19168020341672065 1.0400524914583953 >10 0.8490773172878994 8.876248924781226 >50 0.04250949915077923 1.7567756614592005 >100 0.019591576306035214 2.1590629504993486 >500 9.477427296948844E-4 0.3776802415117465 >1k 4.8739132382485473E-4 0.5574828141996137 >5k 2.7077295658587417E-5 0.09949803060138268 >10k+ 8.123188697576224E-5 0.9561605657726312 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24328 0.30446646344706646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20511 0.2566964662842314 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13733 0.17186936626597188 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 2.5030126886473733E-5 3 0.0 0.0 0.0 0.0 2.5030126886473733E-5 4 0.0 0.0 0.0 0.0 3.75451903297106E-5 5 0.0 1.2515063443236866E-5 0.0 0.0 3.75451903297106E-5 6 0.0 1.2515063443236866E-5 0.0 0.0 3.75451903297106E-5 7 0.0 1.2515063443236866E-5 0.0 0.0 3.75451903297106E-5 8 0.0 1.2515063443236866E-5 0.0 0.0 3.75451903297106E-5 9 0.0 1.2515063443236866E-5 0.0 0.0 3.75451903297106E-5 10-11 0.0 1.2515063443236866E-5 0.0 6.257531721618433E-6 3.75451903297106E-5 12-13 0.0 1.87725951648553E-5 0.0 1.2515063443236866E-5 4.380272205132903E-5 14-15 0.0 2.5030126886473733E-5 0.0 1.87725951648553E-5 7.50903806594212E-5 16-17 0.0 2.5030126886473733E-5 0.0 3.128765860809216E-5 8.760544410265806E-5 18-19 0.0 2.5030126886473733E-5 0.0 4.380272205132903E-5 1.0012050754589493E-4 20-21 0.0 2.5030126886473733E-5 0.0 1.3766569787560553E-4 1.0012050754589493E-4 22-23 1.2515063443236866E-5 2.5030126886473733E-5 0.0 4.567998156781456E-4 1.0637803926751336E-4 24-25 1.2515063443236866E-5 2.5030126886473733E-5 0.0 0.001145128305056173 1.1889310271075023E-4 26-27 1.2515063443236866E-5 2.5030126886473733E-5 0.0 0.002296514141833965 1.2515063443236865E-4 28-29 1.2515063443236866E-5 2.5030126886473733E-5 0.0 0.006482802863596697 1.2515063443236865E-4 30-31 2.5030126886473733E-5 2.5030126886473733E-5 0.0 0.02187633089877804 1.314081661539871E-4 32-33 2.5030126886473733E-5 2.5030126886473733E-5 0.0 0.056981083857057455 1.7521088820531612E-4 34-35 2.5030126886473733E-5 2.5030126886473733E-5 0.0 0.108630750687296 1.7521088820531612E-4 36-37 2.5030126886473733E-5 2.5030126886473733E-5 0.0 0.1893529098961738 1.7521088820531612E-4 38-39 2.5030126886473733E-5 3.128765860809216E-5 0.0 0.30827730026553213 1.7521088820531612E-4 40-41 2.5030126886473733E-5 3.75451903297106E-5 0.0 0.4442659796397439 1.7521088820531612E-4 42-43 2.5030126886473733E-5 3.75451903297106E-5 0.0 0.5721448979027381 1.7521088820531612E-4 44-45 2.5030126886473733E-5 3.75451903297106E-5 0.0 0.7227323987834857 1.8146841992693454E-4 46-47 2.5030126886473733E-5 3.75451903297106E-5 0.0 0.8948645813817657 1.9398348337017142E-4 48-49 2.5030126886473733E-5 3.75451903297106E-5 0.0 1.0820649003657028 2.252711419782636E-4 50-51 2.5030126886473733E-5 3.75451903297106E-5 0.0 1.2859978591732473 2.252711419782636E-4 52-53 2.5030126886473733E-5 3.75451903297106E-5 0.0 1.4871900190867233 2.565588005863557E-4 54-55 2.5030126886473733E-5 3.75451903297106E-5 0.0 1.7159653788290932 2.878464591944479E-4 56-57 2.5030126886473733E-5 5.0060253772947465E-5 0.0 1.97194848649706 2.878464591944479E-4 58-59 2.5030126886473733E-5 7.50903806594212E-5 0.0 2.2577237026916523 2.878464591944479E-4 60-61 2.5030126886473733E-5 9.38629758242765E-5 0.0 2.5412274348712973 2.878464591944479E-4 62-63 2.5030126886473733E-5 1.126355709891318E-4 0.0 2.8378094083491243 3.003615226376848E-4 64-65 2.5030126886473733E-5 1.2515063443236865E-4 0.0 3.1298797014556645 3.003615226376848E-4 66-67 2.5030126886473733E-5 1.2515063443236865E-4 0.0 3.4612848389642985 3.1287658608092166E-4 68-69 2.5030126886473733E-5 1.2515063443236865E-4 0.0 3.8317119442889447 3.1287658608092166E-4 70-71 2.5030126886473733E-5 1.314081661539871E-4 0.0 4.220692631168189 3.1287658608092166E-4 72-73 2.5030126886473733E-5 1.3766569787560553E-4 0.0 4.587415277713638 3.6293683985386913E-4 74-75 2.5030126886473733E-5 1.3766569787560553E-4 0.0 4.951497245872563 3.6293683985386913E-4 76-77 2.5030126886473733E-5 1.3766569787560553E-4 0.0 5.355790112874608 3.6919437157548754E-4 78-79 3.128765860809216E-5 1.3766569787560553E-4 0.0 5.8007444210037304 3.7545190329710596E-4 80-81 3.75451903297106E-5 1.6269582476207927E-4 0.0 6.246937720413733 3.817094350187244E-4 82-83 3.75451903297106E-5 1.6269582476207927E-4 0.0 6.698424891660224 4.380272205132903E-4 84-85 3.75451903297106E-5 1.6269582476207927E-4 0.0 7.1512261445682554 4.380272205132903E-4 86-87 3.75451903297106E-5 1.6269582476207927E-4 0.0 7.62109168648114 4.442847522349088E-4 88 3.75451903297106E-5 1.6269582476207927E-4 0.0 7.989729137733405 4.505422839565272E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11970 0.0 54.22461 1 GTATCAA 21765 0.0 40.969753 1 TACCTGG 7280 0.0 34.738327 2 ATCAACG 27275 0.0 31.929884 3 CAACGCA 27355 0.0 31.829332 5 TATCAAC 28285 0.0 31.30165 2 TCAACGC 28185 0.0 30.899168 4 AACGCAG 29005 0.0 30.32578 6 GTACATG 28420 0.0 28.66778 1 GTACCTG 9110 0.0 28.076733 1 TACATGG 28855 0.0 27.821493 2 ACCTGGG 8900 0.0 27.62647 3 ACGCAGA 33095 0.0 26.49296 7 ACATGGG 29630 0.0 26.224838 3 GTGGTAT 5570 0.0 25.614553 1 CGCAGAG 34135 0.0 25.589588 8 CATGGGG 21495 0.0 24.90778 4 ATGGGGG 14390 0.0 23.73323 5 TGGTATC 6010 0.0 22.717997 2 GCAGAGT 38350 0.0 22.470554 9 >>END_MODULE