FastQCFastQC Report
Mon 6 Jun 2016
ERR1211366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5409877
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213650.39492579960690416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136390.25211294083026287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109620.20262937586196508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61200.11312641673738608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79800.048.0404851
GTATCAA146650.042.4035381
TATCAAC187900.033.092782
ATCAACG189850.032.522763
CAACGCA194450.031.7041745
TCAACGC195900.031.5180634
GTGGTAT37600.030.524931
AACGCAG207150.029.9875586
ACGCAGA231850.026.7127727
CGCAGAG242900.025.3418528
TGGTATC45100.024.3000952
GCAGAGT256650.023.3627579
GTACATG139450.023.1734621
CCGTATC18350.022.29560794
TACATGG144450.022.011912
GAGTACT162650.020.4411412-13
ACATGGG151600.020.3332713
AGAGTAC235150.019.35617310-11
CAGAGTA247000.019.30278410-11
CATGGGG108450.019.1943234
Produced by FastQC (version 0.11.5)