FastQCFastQC Report
Mon 6 Jun 2016
ERR1211366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5409877
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186600.34492466279732426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123390.2280828196278769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104240.19268460262590073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59390.10978068447766925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73100.049.565381
GTATCAA142900.044.436941
TATCAAC183850.034.3995062
ATCAACG186150.033.800243
TCAACGC191700.032.9195754
CAACGCA191900.032.827265
GTGGTAT34800.031.2886831
AACGCAG204850.030.8888366
ACGCAGA233650.026.9810647
CGCAGAG243300.025.9109138
TGGTATC40500.025.8330552
GCAGAGT259750.023.6555619
GTACATG133150.022.8408571
TACATGG139000.021.9056342
ACATGGG144900.020.4321943
CATGGGG106950.019.9172384
AGAGTAC239100.019.66483110-11
GAGTACT171050.019.56357812-13
AGTACTT180950.019.19304512-13
CAGAGTA250650.019.0393510-11
Produced by FastQC (version 0.11.5)