##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211366_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5409877 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.039465407439025 33.0 31.0 34.0 30.0 34.0 2 32.17240595303738 33.0 31.0 34.0 30.0 34.0 3 32.24397634179113 34.0 31.0 34.0 30.0 34.0 4 35.901094793837274 37.0 35.0 37.0 35.0 37.0 5 35.78415073022917 37.0 35.0 37.0 35.0 37.0 6 35.78681881307098 37.0 35.0 37.0 33.0 37.0 7 35.70045806956424 37.0 35.0 37.0 33.0 37.0 8 35.68209572971807 37.0 35.0 37.0 33.0 37.0 9 37.282394590486994 39.0 37.0 39.0 34.0 39.0 10-11 37.242578528125506 39.0 37.0 39.0 33.0 39.0 12-13 37.17520370611014 39.0 37.0 39.0 33.0 39.0 14-15 38.39573589196205 40.0 38.0 41.0 33.0 41.0 16-17 38.375071928622404 40.0 38.0 41.0 33.0 41.0 18-19 38.36727655730435 40.0 38.0 41.0 33.5 41.0 20-21 38.330502523439996 40.0 38.0 41.0 34.0 41.0 22-23 38.21698228998552 40.0 38.0 41.0 33.0 41.0 24-25 38.12097345281603 40.0 37.0 41.0 33.0 41.0 26-27 38.001623234687216 40.0 37.0 41.0 33.0 41.0 28-29 37.86443906210806 40.0 37.0 41.0 33.0 41.0 30-31 37.863458633163006 40.0 37.0 41.0 33.0 41.0 32-33 37.792732256204715 40.0 37.0 41.0 33.0 41.0 34-35 37.64618881353495 40.0 37.0 41.0 32.5 41.0 36-37 37.48270635358253 40.0 36.0 41.0 32.0 41.0 38-39 37.359899864636475 40.0 36.0 41.0 31.5 41.0 40-41 37.2341434010422 40.0 36.0 41.0 31.0 41.0 42-43 37.1050647731917 39.0 36.0 41.0 31.0 41.0 44-45 36.93781559174081 39.0 35.5 41.0 31.0 41.0 46-47 36.693586841253506 39.0 35.0 41.0 30.0 41.0 48-49 35.115062320270866 39.0 35.0 40.5 23.0 41.0 50-51 35.85155531262541 38.5 34.5 40.0 27.5 41.0 52-53 35.951806667693184 38.0 35.0 40.0 28.5 41.0 54-55 35.77576246188222 38.0 35.0 40.0 28.0 41.0 56-57 35.55445992579868 38.0 34.0 40.0 28.0 41.0 58-59 35.30310901708116 38.0 34.0 40.0 27.0 41.0 60-61 35.0281680156499 37.0 34.0 40.0 27.0 41.0 62-63 34.69397853222911 37.0 34.0 40.0 26.0 41.0 64-65 34.337937997481276 36.0 33.0 39.0 26.0 41.0 66-67 33.9745543198117 36.0 33.0 39.0 26.0 40.0 68-69 33.58895350116093 35.0 33.0 38.5 25.5 40.0 70-71 33.19890045559261 35.0 32.0 37.5 25.0 40.0 72-73 32.80209587020185 35.0 32.0 37.0 24.0 39.0 74-75 32.42271275298866 35.0 31.5 36.5 24.0 39.0 76-77 31.412470745638025 34.0 30.5 35.0 22.5 37.0 78-79 31.83374400194311 34.0 31.0 35.0 23.0 37.0 80-81 31.69449444044661 34.0 31.0 35.0 23.0 37.0 82-83 31.42402322640607 34.0 31.0 35.0 22.5 36.5 84-85 31.159493367409276 34.0 31.0 35.0 20.5 36.0 86-87 30.906417280836514 34.0 30.0 35.0 20.0 35.5 88-89 30.67666714049136 34.0 30.0 35.0 20.0 35.0 90-91 30.470654138717016 34.0 30.0 35.0 19.0 35.0 92-93 30.292561549920634 34.0 29.5 35.0 19.0 35.0 94-95 30.106232174964422 34.0 29.0 35.0 18.0 35.0 96-97 29.92036232986443 34.0 29.0 35.0 18.0 35.0 98-99 29.681007534921775 33.0 29.0 35.0 17.5 35.0 100 27.170607206041836 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26334074650529615 1101 2 -0.18714935471573924 1101 3 -0.26181182305118966 1101 4 -0.15106859846621035 1101 5 -0.08854576822827909 1101 6 -0.16010513362583367 1101 7 -0.1701852860590094 1101 8 -0.16929339529255572 1101 9 -0.273989954998477 1101 10-11 -0.31656744390456737 1101 12-13 -0.3688570344592108 1101 14-15 -0.46897943860662394 1101 16-17 -0.4318902783473959 1101 18-19 -0.46500850925761483 1101 20-21 -0.4937096193175634 1101 22-23 -0.6134648794891859 1101 24-25 -0.5452636411873613 1101 26-27 -0.6287553259312446 1101 28-29 -0.6342096152478902 1101 30-31 -0.44858293952705 1101 32-33 -0.406334491858658 1101 34-35 -0.5284929761483923 1101 36-37 -0.4689273498904072 1101 38-39 -0.4672300876060902 1101 40-41 -0.46948242808245766 1101 42-43 -0.5006047383267713 1101 44-45 -0.4562629653049939 1101 46-47 -0.4897586437425758 1101 48-49 0.7439517108251721 1101 50-51 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1105 68-69 -0.6562319967810097 1105 70-71 -0.6996571702321859 1105 72-73 -0.6099818767069465 1105 74-75 -0.784794306341638 1105 76-77 -0.8175175303314255 1105 78-79 -0.6799197397758867 1105 80-81 -0.6439383881089356 1105 82-83 -0.5514856027507378 1105 84-85 -0.5556175495619442 1105 86-87 -0.553266375200316 1105 88-89 -0.6248618846593352 1105 90-91 -0.6873571485091077 1105 92-93 -0.6480009670655029 1105 94-95 -0.6727812930069561 1105 96-97 -0.7092751635236318 1105 98-99 -0.8163631498640136 1105 100 -0.94773634732638 1106 1 -0.33613735208902185 1106 2 -0.24580640535505793 1106 3 -0.3333196868034456 1106 4 -0.24796216963786577 1106 5 -0.21394404873871764 1106 6 -0.36758898754879965 1106 7 -0.306037136849838 1106 8 -0.3527785606366649 1106 9 -0.450773583242416 1106 10-11 -0.5143525108920883 1106 12-13 -0.47826560580593735 1106 14-15 -0.5581930792963306 1106 16-17 -0.5168083781872852 1106 18-19 -0.5958235638630214 1106 20-21 -0.5609358681119758 1106 22-23 -0.6407431156277497 1106 24-25 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2311 4 0.2665071110620829 2311 5 0.3330730680301315 2311 6 0.37276112925128047 2311 7 0.4083469418598469 2311 8 0.24161213556319439 2311 9 0.49204676797401703 2311 10-11 0.3806505688718289 2311 12-13 0.5046839905179752 2311 14-15 0.49798666317924045 2311 16-17 0.43980037920736237 2311 18-19 0.5681167834587626 2311 20-21 0.6668372775539027 2311 22-23 0.6880572270960386 2311 24-25 0.596854927924376 2311 26-27 0.7009985187718826 2311 28-29 0.7553565987021145 2311 30-31 0.6111951399065916 2311 32-33 0.6501052751312599 2311 34-35 0.7686112457477421 2311 36-37 0.7161495040097776 2311 38-39 0.7886124527614768 2311 40-41 0.698230887883895 2311 42-43 0.7536736330596483 2311 44-45 0.8718455589839493 2311 46-47 0.9087994156105879 2311 48-49 -5.846882223845512 2311 50-51 -1.0444070914050485 2311 52-53 0.48618975024435684 2311 54-55 0.8627089083086119 2311 56-57 0.9612706987854907 2311 58-59 0.9300610416097541 2311 60-61 0.7888175408168365 2311 62-63 0.6652654194043421 2311 64-65 0.7954069416191771 2311 66-67 0.8904420572023923 2311 68-69 0.936275600892472 2311 70-71 0.9896835605147345 2311 72-73 0.46206737148733623 2311 74-75 0.8386688755655243 2311 76-77 0.8680197591238148 2311 78-79 0.7819610223224771 2311 80-81 0.8323400558556635 2311 82-83 0.80820190363767 2311 84-85 0.8862263428138135 2311 86-87 0.9131553565330179 2311 88-89 0.96051657715525 2311 90-91 1.0566079007886628 2311 92-93 1.089247445621453 2311 94-95 1.1482256936980413 2311 96-97 1.184727421034058 2311 98-99 1.224850477362068 2311 100 1.4016291421926503 2312 1 0.038810228018107296 2312 2 0.19978889401018307 2312 3 0.21065583787202513 2312 4 -0.032587891602751995 2312 5 0.20858888048489632 2312 6 0.11577961007610327 2312 7 0.29893846407274793 2312 8 0.35472558530031506 2312 9 0.32295833189454726 2312 10-11 0.20109801425992657 2312 12-13 0.3007607185111709 2312 14-15 0.27844866409399316 2312 16-17 0.4189998889063631 2312 18-19 0.25943200694194957 2312 20-21 0.41601367535840694 2312 22-23 0.6229039269371981 2312 24-25 0.7478419519960013 2312 26-27 0.6411282730029271 2312 28-29 0.7719016551992013 2312 30-31 0.7569014970241383 2312 32-33 0.7525669968297137 2312 34-35 0.2519176755848136 2312 36-37 0.6464202599472415 2312 38-39 0.7697011442081632 2312 40-41 0.5928429151640202 2312 42-43 0.5775281135667569 2312 44-45 0.6728221642351642 2312 46-47 0.7549045199837252 2312 48-49 -4.767871172518827 2312 50-51 -0.9640623097990826 2312 52-53 0.38886178608994726 2312 54-55 0.7506183927093844 2312 56-57 0.8135385708015477 2312 58-59 0.8499961836947776 2312 60-61 0.9149939782350955 2312 62-63 0.6458893566796107 2312 64-65 0.8589514005751866 2312 66-67 0.8317384591016648 2312 68-69 0.7243930094781845 2312 70-71 0.6930440292666589 2312 72-73 0.8828683443282657 2312 74-75 0.565094728024107 2312 76-77 0.770404418436474 2312 78-79 0.5468630303353663 2312 80-81 0.7497318335166234 2312 82-83 0.7519960181460625 2312 84-85 0.7372097364671681 2312 86-87 0.806844747243229 2312 88-89 0.8484871243322942 2312 90-91 0.6881723134531796 2312 92-93 0.8167140432470141 2312 94-95 0.9649126497795244 2312 96-97 1.070589660278067 2312 98-99 1.0608310601571276 2312 100 0.7091877184987361 2313 1 0.05555217146662983 2313 2 0.22444745485457673 2313 3 0.273652209940316 2313 4 0.07680716999225012 2313 5 0.15837668089422863 2313 6 0.28730647872004056 2313 7 0.19004383876194453 2313 8 0.22976039999908693 2313 9 0.4228768925108355 2313 10-11 0.23407913889074905 2313 12-13 0.014103967030699494 2313 14-15 0.09465367835101546 2313 16-17 0.05883626532448005 2313 18-19 0.4104479533745433 2313 20-21 0.32304817532429553 2313 22-23 0.42679563001424015 2313 24-25 0.25652879458093736 2313 26-27 0.38503777659533966 2313 28-29 0.35213968169399124 2313 30-31 0.5666233490900794 2313 32-33 0.5303639326960337 2313 34-35 0.4692048267200235 2313 36-37 -0.44717160453794236 2313 38-39 0.14011422010880636 2313 40-41 0.37171049055434935 2313 42-43 0.467155564276446 2313 44-45 0.4341080541378801 2313 46-47 0.460591385978546 2313 48-49 -5.392593742888039 2313 50-51 -1.3435266321359762 2313 52-53 0.06807456222403374 2313 54-55 0.28483341329107503 2313 56-57 0.2683939357366185 2313 58-59 0.437511745264878 2313 60-61 0.7279438306627881 2313 62-63 0.8245529759596693 2313 64-65 0.9608272500588342 2313 66-67 0.9529605015479845 2313 68-69 0.9279092965545956 2313 70-71 0.9124517131908121 2313 72-73 0.6888916723773661 2313 74-75 0.787592241474286 2313 76-77 0.8114621405067304 2313 78-79 0.8096420631430163 2313 80-81 -0.22935562470824422 2313 82-83 0.2709985785270561 2313 84-85 0.6796767335333271 2313 86-87 0.8089011529577199 2313 88-89 0.8113010743820048 2313 90-91 0.7967410761848015 2313 92-93 0.9184644022485848 2313 94-95 0.7790574942328554 2313 96-97 0.8562964231733581 2313 98-99 1.0657495367379397 2313 100 1.0980272365432562 2314 1 0.04831085821040659 2314 2 0.12254436124506896 2314 3 0.22229916179286846 2314 4 0.08550846588772032 2314 5 0.1756485422135441 2314 6 0.25826794518562224 2314 7 0.06255197418305158 2314 8 0.22745234532600023 2314 9 0.39110055509810593 2314 10-11 0.23816975725077327 2314 12-13 0.13272633308879733 2314 14-15 0.2250680773080731 2314 16-17 0.41384015824451126 2314 18-19 0.5347447021006104 2314 20-21 0.523007992158746 2314 22-23 0.23165214102665743 2314 24-25 0.39770183668047565 2314 26-27 0.460224411287399 2314 28-29 0.6496160746653388 2314 30-31 0.487902966741494 2314 32-33 0.4870470527741162 2314 34-35 0.3829830770598974 2314 36-37 0.434674498611308 2314 38-39 0.5062921673536422 2314 40-41 0.265921095411187 2314 42-43 0.18911169437946995 2314 44-45 0.4480437437988627 2314 46-47 0.5559457833682018 2314 48-49 -6.419053560982569 2314 50-51 -1.6458964381780063 2314 52-53 -0.8749047967239463 2314 54-55 -0.4304609435053379 2314 56-57 -0.10316472161665757 2314 58-59 0.35568882028814386 2314 60-61 0.5882992805269396 2314 62-63 0.48923350021944856 2314 64-65 0.5482711693657123 2314 66-67 0.6610394318257775 2314 68-69 0.6565488660380865 2314 70-71 0.46306138623275217 2314 72-73 0.695373143822593 2314 74-75 0.6534777028389556 2314 76-77 0.5676317293693067 2314 78-79 0.4172340364829097 2314 80-81 0.4687958762735569 2314 82-83 0.512497299274461 2314 84-85 0.5537159055501846 2314 86-87 0.6244230610285335 2314 88-89 0.4900360051749857 2314 90-91 0.7198338420430055 2314 92-93 0.7459340428919781 2314 94-95 0.8044619017166283 2314 96-97 0.8039500876918595 2314 98-99 0.9351243003348948 2314 100 -0.4858524117562979 2315 1 0.16485977857681888 2315 2 0.23534212512833363 2315 3 0.2846198715577515 2315 4 0.12052203446572207 2315 5 0.2260489891548474 2315 6 0.32872155099534695 2315 7 0.34656775815052043 2315 8 0.3162246882596804 2315 9 0.4881994787611461 2315 10-11 0.29024179042361453 2315 12-13 0.47202718352639295 2315 14-15 0.3307081258656055 2315 16-17 0.42379660624416005 2315 18-19 0.5070815297315505 2315 20-21 0.41388239049310016 2315 22-23 0.5032027082392716 2315 24-25 0.5248802293336112 2315 26-27 0.5608201869438787 2315 28-29 0.6580891614182249 2315 30-31 0.63105497185785 2315 32-33 0.7210790285461712 2315 34-35 0.6726404079419623 2315 36-37 0.5680984817306012 2315 38-39 0.6858537593403895 2315 40-41 0.7065080919684164 2315 42-43 0.6821677887301334 2315 44-45 0.7409357631549938 2315 46-47 0.6621730067189162 2315 48-49 -4.985306637847771 2315 50-51 -0.9558616731114498 2315 52-53 0.20851653856339425 2315 54-55 0.1925001248937548 2315 56-57 0.5497888278210539 2315 58-59 0.6789574950258412 2315 60-61 0.5115664691490664 2315 62-63 0.31033603296436496 2315 64-65 0.5307315167049609 2315 66-67 -0.17402897613091284 2315 68-69 0.5732775623202926 2315 70-71 0.5786516273940592 2315 72-73 0.6613324668070959 2315 74-75 0.8326693818060633 2315 76-77 0.8339028403817288 2315 78-79 0.6399160432246873 2315 80-81 0.500405652583293 2315 82-83 0.5764597383030186 2315 84-85 0.645597008861003 2315 86-87 0.6059920205740426 2315 88-89 0.5569841042654531 2315 90-91 0.6437057107736095 2315 92-93 0.7809102994412598 2315 94-95 0.3853856202363488 2315 96-97 0.571351246377251 2315 98-99 0.5133398608855728 2315 100 0.7070123874465963 2316 1 0.20458965277455832 2316 2 0.22180522898452892 2316 3 0.1651939918839176 2316 4 -0.04331912687255368 2316 5 0.17132755370368358 2316 6 0.17585272495360726 2316 7 0.26404962767720264 2316 8 0.2738897868675991 2316 9 0.5120987861886519 2316 10-11 0.4201112825492359 2316 12-13 0.3304791841784436 2316 14-15 0.5063128396532122 2316 16-17 0.45042584227199 2316 18-19 0.13047662598641807 2316 20-21 0.47814476794175675 2316 22-23 0.556796016130555 2316 24-25 0.6305537014977602 2316 26-27 0.639021902995232 2316 28-29 0.6723144735966926 2316 30-31 0.5345365958040844 2316 32-33 0.5020724856650389 2316 34-35 0.48007891575201 2316 36-37 0.3682450538661257 2316 38-39 0.5033829095712647 2316 40-41 0.0507951870819312 2316 42-43 -0.02492442916908999 2316 44-45 0.24797391772164445 2316 46-47 0.2824205021192867 2316 48-49 -5.34889834050886 2316 50-51 -1.1764158951243289 2316 52-53 0.23578534056939304 2316 54-55 0.49212602840319164 2316 56-57 0.37657077427529373 2316 58-59 0.20244645221612956 2316 60-61 0.422045012503105 2316 62-63 0.32959715993455774 2316 64-65 0.29942398532956815 2316 66-67 0.4144518266511241 2316 68-69 0.05222166255266103 2316 70-71 -0.2703900316363601 2316 72-73 -0.1183632360256226 2316 74-75 0.05974376229570311 2316 76-77 0.1617669443326939 2316 78-79 0.2478831982186236 2316 80-81 0.13589863678528502 2316 82-83 -0.023080104659729983 2316 84-85 0.14245824324029854 2316 86-87 0.14855140280278079 2316 88-89 -0.027889409233917917 2316 90-91 0.07466820674375185 2316 92-93 0.2982832124742245 2316 94-95 0.46578158612717147 2316 96-97 0.5499991832269053 2316 98-99 0.5781601272131986 2316 100 -0.1526597907218985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 6.0 11 10.0 12 40.0 13 209.0 14 856.0 15 2529.0 16 5657.0 17 10618.0 18 16608.0 19 23411.0 20 31148.0 21 39884.0 22 47204.0 23 49317.0 24 51641.0 25 57313.0 26 67086.0 27 80429.0 28 97865.0 29 119104.0 30 143984.0 31 175642.0 32 217080.0 33 278649.0 34 378799.0 35 531912.0 36 814320.0 37 1191260.0 38 888506.0 39 88784.0 40 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.43003427357621 19.652445671870076 14.306111701978164 24.61140835257555 2 16.372658751981366 21.394494225730277 38.125522780522296 24.10732424176606 3 19.064835372229762 24.608202302790584 30.202439207884996 26.12452311709466 4 14.270793330721396 17.344251207497237 35.84878693745273 32.536168524328644 5 14.839092848815469 35.99951495963204 33.56494134135474 15.59645085019776 6 31.80901591996118 35.24220060537443 17.723773654659304 15.225009820005084 7 27.440639098064025 32.102540650669496 21.94942063345653 18.507399617809952 8 25.6484691100963 33.79222813098928 21.027683050185743 19.531619708728677 9 26.066982935741624 16.95570495208453 21.00686260264233 35.97044950953151 10-11 25.0941943941604 25.87320324528178 27.748061258196433 21.28454110236139 12-13 25.576832199587773 23.733654992296028 27.641132999394348 23.048379808721855 14-15 23.238247869172458 25.538025867534706 25.742522463571216 25.48120379972162 16-17 22.706356791705957 27.827330820698766 26.690933470638196 22.775378916957084 18-19 22.425671124439823 27.10506673350739 28.61493498187389 21.8543271601789 20-21 23.03167958209736 26.50061531024992 28.408063221453002 22.05964188619972 22-23 23.104331687297645 26.133824189561793 28.073853578457065 22.687990544683494 24-25 22.80524544370333 26.095598124615055 28.28553741342685 22.813619018254762 26-27 22.74408710594262 26.349348006755058 28.266389666890458 22.64017522041187 28-29 22.57638827401014 26.70677760794288 28.157793896997486 22.5590402210495 30-31 23.134359327757405 26.333891800969862 28.09596240561128 22.43578646566145 32-33 22.84889917152478 26.450201364862778 27.903217308597085 22.79768215501536 34-35 23.048294009887705 26.384509354457524 28.050957705614614 22.516238930040156 36-37 22.920508674521503 26.078818241293238 28.106113418151864 22.894559666033395 38-39 22.684017364132522 26.065953862968527 28.24025134895208 23.009777423946872 40-41 22.82463865366023 25.692995639258136 28.44629854537186 23.036067161709767 42-43 22.52235065679649 25.780661278311438 28.273420254274395 23.42356781061768 44-45 22.105673165008614 25.961078347703825 28.495580521741253 23.437667965546307 46-47 22.031221634300575 25.837757535131413 28.353646024171137 23.77737480639688 48-49 22.10579584280502 25.99677149407622 28.279155438121688 23.61827722499707 50-51 21.746205253045527 26.092689765079502 28.20431663587669 23.95678834599828 52-53 21.707158007514504 26.221906691274384 27.962093763533897 24.108841537677215 54-55 21.39465009195166 26.024584405856217 28.101630801698686 24.47913470049344 56-57 21.480193205636894 25.879182792323718 27.826508680749562 24.814115321289822 58-59 21.304480163877347 25.807779644791307 27.79732139069102 25.09041880064033 60-61 21.569027429849474 25.77572517152148 27.618625001721476 25.03662239690757 62-63 21.47353820924584 25.737052404122068 27.451213099547005 25.338196287085086 64-65 21.207579548644816 25.63032640335658 27.36163888608741 25.800455161911202 66-67 21.3603844872351 25.852155169066478 26.96201389482108 25.825446448877347 68-69 21.259930893416385 26.065394704910265 26.796417551685487 25.878256849987867 70-71 21.291706611765076 26.028993438988696 26.539988702894448 26.13931124635178 72-73 21.293817757178683 26.421897729785538 26.20028058747355 26.08400392556223 74-75 21.35870918043221 26.588976774465124 26.043876278002454 26.00843776710021 76-77 21.240524774334244 26.5194470213747 25.97147291992429 26.26855528436677 78-79 21.12335194491782 26.628131594658193 25.888637918366662 26.359878542057324 80-81 20.951529664642322 26.7994539100508 25.7949955556944 26.454020869612478 82-83 21.056242441004244 26.476571596330494 25.90929796687741 26.557887995787855 84-85 21.074103173742554 26.396371935918157 25.676113540722863 26.85341134961643 86-87 20.865633176457514 26.420384975457278 25.87778921115059 26.83619263693462 88-89 20.72479617892635 26.10516587715846 25.948456887970266 27.221581055944927 90-91 20.849010460909167 25.878365180308116 25.829805026313352 27.442819332469366 92-93 20.88525543915169 25.751867032821675 25.848249398777206 27.51462812924943 94-95 20.703276389155263 25.547627685361274 26.09742514956223 27.651670775921232 96-97 20.653610042787083 25.44804063298595 26.112431323512137 27.785918000714826 98-99 20.557480016246938 25.52649565475736 25.97022676385496 27.94579756514074 100 20.292920685852245 25.698452534962385 26.11057854821876 27.89804823096661 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3561.0 1 2737.0 2 2428.0 3 2941.0 4 2453.0 5 2165.0 6 2920.5 7 4707.5 8 6643.0 9 7011.5 10 6005.5 11 5105.5 12 5003.0 13 5244.0 14 5619.5 15 6084.5 16 6536.0 17 7100.5 18 7744.5 19 8449.0 20 9359.0 21 10332.0 22 11501.5 23 13092.0 24 14745.5 25 16643.5 26 19151.0 27 21922.0 28 24572.0 29 27874.0 30 32450.0 31 37467.0 32 44098.0 33 51732.0 34 60010.0 35 70264.5 36 83374.0 37 98281.0 38 112056.0 39 123785.5 40 133941.0 41 144924.5 42 158530.0 43 174754.0 44 190707.0 45 206844.5 46 223721.0 47 238607.5 48 250840.0 49 259391.5 50 261865.0 51 259067.5 52 252981.5 53 243824.5 54 229555.5 55 208912.0 56 183900.0 57 157299.5 58 134266.5 59 112945.0 60 91517.0 61 73467.5 62 58896.0 63 45590.5 64 33732.0 65 24531.5 66 17864.5 67 13963.0 68 10619.5 69 7767.5 70 6103.5 71 4663.5 72 3585.0 73 2850.0 74 2198.5 75 1595.0 76 1077.0 77 631.5 78 348.5 79 228.5 80 146.5 81 88.0 82 62.5 83 33.5 84 15.0 85 7.5 86 6.5 87 6.0 88 3.5 89 1.5 90 1.5 91 1.5 92 2.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030111590337451297 2 0.0018854402789564345 3 1.1090825170331968E-4 4 0.025434959057294645 5 3.3272475510995905E-4 6 3.6969417234439894E-5 7 5.545412585165984E-5 8 0.003937242935467849 9 0.01033295211702595 10-11 9.98174265329877E-4 12-13 1.2015060601192965E-4 14-15 1.2939296032053963E-4 16-17 3.234824008013491E-4 18-19 1.8484708617219947E-5 20-21 8.318118877748976E-5 22-23 1.9408944048080942E-4 24-25 1.8484708617219947E-5 26-27 9.242354308609974E-5 28-29 8.872660136265574E-4 30-31 0.002467708600398863 32-33 0.010018712070533212 34-35 0.019196369898982917 36-37 0.022043015026034788 38-39 0.02110029488655657 40-41 0.004565723028453327 42-43 0.004593450091379157 44-45 0.008715540113019206 46-47 0.011368095799590269 48-49 1.2299447843268896 50-51 0.016996689573533744 52-53 0.01856788980599744 54-55 0.009482655520633832 56-57 0.007532518761517128 58-59 0.008604631861315885 60-61 0.005129506641278535 62-63 0.007532518761517128 64-65 0.019085461647279596 66-67 0.028300088892963742 68-69 0.02891932663164061 70-71 0.026100408567514565 72-73 0.022643768056094436 74-75 0.009380989623239123 76-77 0.014251710343876579 78-79 0.007994636476947627 80-81 0.0026895251038055023 82-83 0.01402989384046994 84-85 0.023336944629240183 86-87 0.023041189291364665 88-89 0.03259778364646738 90-91 0.02073060071421217 92-93 0.012800660717424814 94-95 0.006275558575546172 96-97 0.00339194403125986 98-99 0.015859879993574716 100 0.03817092329455919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5409877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.9644600014809 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25855270272153 47.1002594601737 2 13.871560719401845 16.91344417272175 3 3.9807230182414397 7.280478876676637 4 1.6043373855912535 3.9123024949103367 5 0.8515105327163239 2.5955939906312016 6 0.5112837821088736 1.870208381026938 7 0.36334147423427327 1.5505641740984117 8 0.2697873644863288 1.3157952792905392 9 0.19541817292957953 1.0722207048415044 >10 1.0272279099580697 11.065535742099634 >50 0.04922381759702484 2.021489876792534 >100 0.016096799654646637 1.6153704320864166 >500 4.6816038675634404E-4 0.19886587976147888 >1k 3.2411074994656726E-4 0.44805167295328285 >5k 3.6012305549507096E-5 0.1303890085096893 >10k+ 1.080369166485213E-4 0.9094298534259739 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18660 0.34492466279732426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12339 0.2280828196278769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10424 0.19268460262590073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5939 0.10978068447766925 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.6969417234439894E-5 5 0.0 1.8484708617219947E-5 0.0 0.0 3.6969417234439894E-5 6 1.8484708617219947E-5 1.8484708617219947E-5 0.0 0.0 3.6969417234439894E-5 7 1.8484708617219947E-5 1.8484708617219947E-5 0.0 1.8484708617219947E-5 3.6969417234439894E-5 8 1.8484708617219947E-5 1.8484708617219947E-5 0.0 5.5454125851659844E-5 5.5454125851659844E-5 9 1.8484708617219947E-5 1.8484708617219947E-5 0.0 5.5454125851659844E-5 9.242354308609974E-5 10-11 1.8484708617219947E-5 3.6969417234439894E-5 0.0 5.5454125851659844E-5 9.242354308609974E-5 12-13 1.8484708617219947E-5 3.6969417234439894E-5 0.0 1.2939296032053963E-4 1.1090825170331969E-4 14-15 1.8484708617219947E-5 3.6969417234439894E-5 0.0 2.0333179478941943E-4 1.386353146291496E-4 16-17 1.8484708617219947E-5 3.6969417234439894E-5 0.0 3.142400464927391E-4 1.6636237755497953E-4 18-19 1.8484708617219947E-5 3.6969417234439894E-5 0.0 4.5287536112188873E-4 2.2181650340663938E-4 20-21 1.8484708617219947E-5 3.6969417234439894E-5 0.0 7.578730533060179E-4 2.3105885771524935E-4 22-23 1.8484708617219947E-5 3.6969417234439894E-5 0.0 0.0021996803254491736 2.9575533787551915E-4 24-25 1.8484708617219947E-5 3.6969417234439894E-5 0.0 0.006543586850495862 3.51209463727179E-4 26-27 1.8484708617219947E-5 3.6969417234439894E-5 0.0 0.015333065797983946 3.7893652665300893E-4 28-29 1.8484708617219947E-5 3.6969417234439894E-5 0.0 0.040962114295759404 4.4363300681327875E-4 30-31 1.8484708617219947E-5 3.6969417234439894E-5 0.0 0.14562253448645876 4.4363300681327875E-4 32-33 1.8484708617219947E-5 3.6969417234439894E-5 0.0 0.4253423876365396 4.8060242404771866E-4 34-35 1.8484708617219947E-5 7.393883446887979E-5 0.0 0.8360910978197841 5.175718412821585E-4 36-37 1.8484708617219947E-5 1.1090825170331968E-4 0.0 1.4178326050666217 5.175718412821585E-4 38-39 1.8484708617219947E-5 1.2939296032053963E-4 0.0 2.1827113629385657 5.452989042079885E-4 40-41 1.8484708617219947E-5 1.2939296032053963E-4 0.0 3.0122403891992366 5.545412585165984E-4 42-43 1.8484708617219947E-5 1.2939296032053963E-4 0.0 3.8306416208723415 5.545412585165984E-4 44-45 1.8484708617219947E-5 1.2939296032053963E-4 0.0 4.784702129087224 5.545412585165984E-4 46-47 1.8484708617219947E-5 1.2939296032053963E-4 0.0 5.766609850833946 5.822683214424284E-4 48-49 1.8484708617219947E-5 1.2939296032053963E-4 0.0 6.699274308824396 6.284800929854782E-4 50-51 1.8484708617219947E-5 1.4787766893775958E-4 0.0 7.654536323099398 6.377224472940881E-4 52-53 1.8484708617219947E-5 1.4787766893775958E-4 0.0 8.584132319459389 7.02418927454358E-4 54-55 1.8484708617219947E-5 1.4787766893775958E-4 0.0 9.59359149940008 7.763577619232378E-4 56-57 1.8484708617219947E-5 1.4787766893775958E-4 0.0 10.68762746361886 7.763577619232378E-4 58-59 1.8484708617219947E-5 1.4787766893775958E-4 0.0 11.798050122026805 7.763577619232378E-4 60-61 1.8484708617219947E-5 1.4787766893775958E-4 0.0 12.918278918356183 7.856001162318477E-4 62-63 1.8484708617219947E-5 1.4787766893775958E-4 0.0 14.05490365122904 8.133271791576777E-4 64-65 1.8484708617219947E-5 1.4787766893775958E-4 0.0 15.250078698646938 8.318118877748977E-4 66-67 1.8484708617219947E-5 1.4787766893775958E-4 0.0 16.53161430472449 8.318118877748977E-4 68-69 1.8484708617219947E-5 1.4787766893775958E-4 0.0 17.86453740075791 8.318118877748977E-4 70-71 1.8484708617219947E-5 1.4787766893775958E-4 0.0 19.249300862108328 8.318118877748977E-4 72-73 1.8484708617219947E-5 1.4787766893775958E-4 0.0 20.618731257660755 9.057507222437775E-4 74-75 1.8484708617219947E-5 1.4787766893775958E-4 0.0 22.01255222623361 9.057507222437775E-4 76-77 1.8484708617219947E-5 1.4787766893775958E-4 0.0 23.4547199501948 9.057507222437775E-4 78-79 1.8484708617219947E-5 1.4787766893775958E-4 0.0 24.93751706369664 9.057507222437775E-4 80-81 1.8484708617219947E-5 1.4787766893775958E-4 0.0 26.43776374213314 9.519624937868273E-4 82-83 1.8484708617219947E-5 1.4787766893775958E-4 0.0 27.936365281502702 0.0010166589739470972 84-85 1.8484708617219947E-5 1.4787766893775958E-4 0.0 29.435050002061047 0.0010166589739470972 86-87 1.8484708617219947E-5 1.4787766893775958E-4 0.0 30.93790302441257 0.0010166589739470972 88 1.8484708617219947E-5 1.4787766893775958E-4 0.0 32.06777159628583 0.0010166589739470972 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7310 0.0 49.56538 1 GTATCAA 14290 0.0 44.43694 1 TATCAAC 18385 0.0 34.399506 2 ATCAACG 18615 0.0 33.80024 3 TCAACGC 19170 0.0 32.919575 4 CAACGCA 19190 0.0 32.82726 5 GTGGTAT 3480 0.0 31.288683 1 AACGCAG 20485 0.0 30.888836 6 ACGCAGA 23365 0.0 26.981064 7 CGCAGAG 24330 0.0 25.910913 8 TGGTATC 4050 0.0 25.833055 2 GCAGAGT 25975 0.0 23.655561 9 GTACATG 13315 0.0 22.840857 1 TACATGG 13900 0.0 21.905634 2 ACATGGG 14490 0.0 20.432194 3 CATGGGG 10695 0.0 19.917238 4 AGAGTAC 23910 0.0 19.664831 10-11 GAGTACT 17105 0.0 19.563578 12-13 AGTACTT 18095 0.0 19.193045 12-13 CAGAGTA 25065 0.0 19.03935 10-11 >>END_MODULE