FastQCFastQC Report
Mon 6 Jun 2016
ERR1211365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594903
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160790.4472721517103521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110890.30846451211618225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84100.23394233446632634No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68590.1907979158269361No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.14022631486857923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54650.049.9940721
GTGGTAT22900.042.5076831
GTATCAA103400.042.0681761
TATCAAC132500.032.5792582
ATCAACG132200.032.270973
TGGTATC30150.032.1287842
CAACGCA133350.031.8512845
TCAACGC142750.029.9522114
AACGCAG145650.029.419546
ACGCAGA164750.025.8102877
CGCAGAG170750.024.7647158
GCAGAGT179550.023.0566649
CGTATCA11850.022.22483394
GTACATG106200.021.2544171
TACATGG109900.020.1956882
GAGTACT111100.020.06952912-13
CCGTATC14750.019.76826394
TATACCG10300.019.614755
ATACCGA11050.019.5590216
TAGTACT9850.019.5574054
Produced by FastQC (version 0.11.5)