##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211365_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3594903 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685918646483646 31.0 31.0 34.0 30.0 34.0 2 31.715946160438822 31.0 31.0 34.0 30.0 34.0 3 31.76638201364543 33.0 31.0 34.0 30.0 34.0 4 35.41714978123193 37.0 35.0 37.0 33.0 37.0 5 35.44861627698995 37.0 35.0 37.0 33.0 37.0 6 35.50381581923073 37.0 35.0 37.0 33.0 37.0 7 35.448015148113875 37.0 35.0 37.0 33.0 37.0 8 35.45715280773918 37.0 35.0 37.0 33.0 37.0 9 37.05844191067186 39.0 37.0 39.0 33.0 39.0 10-11 37.01282746711108 39.0 37.0 39.0 33.0 39.0 12-13 36.94304936739601 39.0 37.0 39.0 33.0 39.0 14-15 38.117081184109836 40.0 38.0 41.0 33.0 41.0 16-17 38.11855424193643 40.0 38.0 41.0 33.0 41.0 18-19 38.10468877741624 40.0 38.0 41.0 33.0 41.0 20-21 38.063220481887825 40.0 38.0 41.0 33.0 41.0 22-23 37.94237897378594 40.0 37.5 41.0 33.0 41.0 24-25 37.82963239898267 40.0 37.0 41.0 32.5 41.0 26-27 37.680646042466236 40.0 37.0 41.0 32.0 41.0 28-29 37.53788780949027 40.0 37.0 41.0 32.0 41.0 30-31 37.335464545218606 39.5 36.0 41.0 31.5 41.0 32-33 37.1985747598753 39.0 36.0 41.0 31.0 41.0 34-35 37.0446859066851 39.0 36.0 41.0 31.0 41.0 36-37 36.92873590747789 39.0 35.0 41.0 31.0 41.0 38-39 36.76037183757114 39.0 35.0 41.0 30.0 41.0 40-41 36.56264883920373 39.0 35.0 40.0 30.0 41.0 42-43 36.37310923271087 39.0 35.0 40.0 30.0 41.0 44-45 36.19997632759493 39.0 35.0 40.0 29.0 41.0 46-47 35.99480305866389 38.5 35.0 40.0 28.0 41.0 48-49 35.72252742285397 38.0 35.0 40.0 27.0 41.0 50-51 34.97758367889203 37.5 33.5 39.5 26.5 40.5 52-53 35.179840040190236 38.0 34.0 40.0 27.0 40.5 54-55 35.51949830078864 38.0 34.0 40.0 27.0 41.0 56-57 35.38619748571797 38.0 34.0 40.0 27.0 41.0 58-59 35.12779093065932 37.5 34.0 40.0 26.0 41.0 60-61 34.82766600378369 37.0 34.0 40.0 26.0 41.0 62-63 34.52387088052167 36.5 33.5 40.0 26.0 41.0 64-65 34.20600333305238 36.0 33.0 39.0 26.0 41.0 66-67 33.942292740582985 35.5 33.0 39.0 25.0 41.0 68-69 33.5446597585526 35.0 33.0 39.0 24.5 40.5 70-71 33.14427663277702 35.0 32.5 37.5 24.0 40.0 72-73 32.71716496940251 35.0 32.0 37.0 23.0 39.5 74-75 32.284555522082236 35.0 31.5 36.5 21.5 39.0 76-77 31.861428667199085 35.0 31.0 36.0 20.0 39.0 78-79 31.418767210130564 35.0 31.0 35.5 19.5 37.5 80-81 31.003430690619467 34.0 30.0 35.0 18.0 37.0 82-83 30.608677063052884 34.0 30.0 35.0 17.5 36.5 84-85 30.232408357054418 34.0 29.0 35.0 15.0 36.0 86-87 29.881547012534135 34.0 29.0 35.0 11.5 36.0 88-89 29.504214160994053 34.0 28.5 35.0 10.0 35.0 90-91 29.122308167981167 34.0 27.0 35.0 10.0 35.0 92-93 28.754127162819138 34.0 26.5 35.0 10.0 35.0 94-95 28.39365206794175 33.0 25.0 35.0 8.0 35.0 96-97 28.0472452246973 33.0 25.0 35.0 8.0 35.0 98-99 27.649576636699237 33.0 24.0 35.0 7.0 35.0 100 25.701017245806074 30.0 19.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4638340895857773 1101 2 -0.4979483007164447 1101 3 -0.3846248519555644 1101 4 -0.438113578906723 1101 5 -0.4005763692381805 1101 6 -0.3946803495481319 1101 7 -0.5001235467147822 1101 8 -0.6121336348643851 1101 9 -0.5895432524171653 1101 10-11 -0.670082695738536 1101 12-13 -0.6337585009896998 1101 14-15 -0.6455728754005676 1101 16-17 -0.7758090665933466 1101 18-19 -0.7094788563152576 1101 20-21 -0.8215430702448572 1101 22-23 -0.8944733382995693 1101 24-25 -0.6871053170434038 1101 26-27 -0.861377209577249 1101 28-29 -0.9185555689429492 1101 30-31 -1.011359022069243 1101 32-33 -0.8767960472084582 1101 34-35 -1.008014699126015 1101 36-37 -0.9918221446425051 1101 38-39 -0.9633952333443929 1101 40-41 -0.9873470270906353 1101 42-43 -1.1396667625429586 1101 44-45 -1.210736129337242 1101 46-47 -1.3595820621412358 1101 48-49 -1.1056748007197044 1101 50-51 -0.9897485158780768 1101 52-53 -0.8528649336647547 1101 54-55 -0.8439140376330982 1101 56-57 -0.981314251107797 1101 58-59 -1.040397990959704 1101 60-61 -0.9410408550308347 1101 62-63 -0.9406275054738273 1101 64-65 -1.076725575043973 1101 66-67 -1.0390473782817793 1101 68-69 -0.9792593057442716 1101 70-71 -1.0824106462766352 1101 72-73 -1.1611918977595437 1101 74-75 -1.3055354109059074 1101 76-77 -1.4992914194225193 1101 78-79 -1.5853369362222445 1101 80-81 -1.6340465920739788 1101 82-83 -1.6847932618689896 1101 84-85 -1.9586991242079748 1101 86-87 -1.9624345052282521 1101 88-89 -1.8613621101156035 1101 90-91 -2.0008907728519247 1101 92-93 -2.114141496361974 1101 94-95 -2.134991648658872 1101 96-97 -2.2151142373312034 1101 98-99 -2.3532627829565804 1101 100 -2.416105367476728 1102 1 -0.4413075611519339 1102 2 -0.3813984782971609 1102 3 -0.45613711355787956 1102 4 -0.29067881929272943 1102 5 -0.388947589409284 1102 6 -0.35819137629632536 1102 7 -0.488252163130781 1102 8 -0.4170609966235119 1102 9 -0.41640275837892204 1102 10-11 -0.493755147060817 1102 12-13 -0.4418571194664622 1102 14-15 -0.6192629962456166 1102 16-17 -0.612143351021949 1102 18-19 -0.5304048842065399 1102 20-21 -0.7155081735411244 1102 22-23 -0.7790636830927369 1102 24-25 -0.7457194810777494 1102 26-27 -0.6787343661767125 1102 28-29 -0.7512664943429428 1102 30-31 -0.5633885720317338 1102 32-33 -0.6207474053064885 1102 34-35 -0.6055504648160692 1102 36-37 -0.672978323099052 1102 38-39 -0.8783255876525331 1102 40-41 -0.8282890722634377 1102 42-43 -0.8150420587791487 1102 44-45 -0.8849054001454206 1102 46-47 -0.8711156519442724 1102 48-49 -0.8860406705271231 1102 50-51 -0.9014632384142445 1102 52-53 -0.6927475621491581 1102 54-55 -0.6602107713071064 1102 56-57 -0.5501671287847927 1102 58-59 -0.6787332985301475 1102 60-61 -0.7852500010012164 1102 62-63 -0.7323236548863505 1102 64-65 -0.8424186972929206 1102 66-67 -0.6669814146125717 1102 68-69 -0.7541894829546933 1102 70-71 -0.7487523989966931 1102 72-73 -0.919640867855783 1102 74-75 -0.9419294356287509 1102 76-77 -0.8938260025827773 1102 78-79 -0.9111949847502423 1102 80-81 -0.9639323559930553 1102 82-83 -1.0281748784814013 1102 84-85 -1.1459152647334072 1102 86-87 -1.158057253269714 1102 88-89 -1.2720798529729294 1102 90-91 -1.3742035145500644 1102 92-93 -1.37878783019762 1102 94-95 -1.2945145353195464 1102 96-97 -1.4302428907767215 1102 98-99 -1.5461716593359291 1102 100 -1.5633553819665416 1103 1 -0.4721512915741144 1103 2 -0.5302392703496857 1103 3 -0.43846968833194566 1103 4 -0.3781462000710505 1103 5 -0.3579993769174976 1103 6 -0.3865286432103048 1103 7 -0.49470460971067354 1103 8 -0.3716318106283367 1103 9 -0.6503785374440909 1103 10-11 -0.5945589249071972 1103 12-13 -0.7231956564560278 1103 14-15 -0.7649233199087391 1103 16-17 -0.8660536854952952 1103 18-19 -0.8824005017921053 1103 20-21 -0.8656639916035473 1103 22-23 -0.9081863756127646 1103 24-25 -0.9283259818937921 1103 26-27 -0.8572803458293592 1103 28-29 -0.7843647849197453 1103 30-31 -0.9968588348659821 1103 32-33 -0.9089823622516349 1103 34-35 -1.0417590830281327 1103 36-37 -1.0393791420614846 1103 38-39 -1.0266365781807707 1103 40-41 -1.0349905562306176 1103 42-43 -0.9781036750433572 1103 44-45 -0.9248936748236574 1103 46-47 -0.862468377981024 1103 48-49 -0.9180080105785535 1103 50-51 -0.9661950593888946 1103 52-53 -0.9036645305802864 1103 54-55 -0.8525082606200698 1103 56-57 -0.7425793070297928 1103 58-59 -0.6498829230885619 1103 60-61 -0.7103339436456935 1103 62-63 -1.0001105456548132 1103 64-65 -0.9067351542747701 1103 66-67 -0.8271194435719025 1103 68-69 -0.7595441136020256 1103 70-71 -0.8306794143995049 1103 72-73 -1.023805016326918 1103 74-75 -0.9605345346353147 1103 76-77 -0.8859169412018275 1103 78-79 -0.8757263389243448 1103 80-81 -0.9362934295337517 1103 82-83 -1.128872062493528 1103 84-85 -0.9711383827237299 1103 86-87 -1.183012126870544 1103 88-89 -1.4114145867749421 1103 90-91 -1.3290055165029457 1103 92-93 -1.2291726238832439 1103 94-95 -1.5228124831871206 1103 96-97 -1.6255322980647229 1103 98-99 -1.5584494086775145 1103 100 -1.4950933944339972 1104 1 -0.531092911987006 1104 2 -0.4943250428210355 1104 3 -0.5578044325718565 1104 4 -0.5126029344878518 1104 5 -0.46509417386674556 1104 6 -0.4267574496632065 1104 7 -0.47598181292115527 1104 8 -0.44838779034322584 1104 9 -0.5065079947774791 1104 10-11 -0.6706021305934229 1104 12-13 -0.663801813865291 1104 14-15 -0.8709324841462518 1104 16-17 -0.777928760366521 1104 18-19 -0.8239894687689144 1104 20-21 -0.8157034237802065 1104 22-23 -0.7684033024985197 1104 24-25 -0.8503401175804157 1104 26-27 -0.8562975362618133 1104 28-29 -0.7995806136942534 1104 30-31 -1.083625596036768 1104 32-33 -0.9734345045668533 1104 34-35 -1.0170720447339932 1104 36-37 -1.1000428005688505 1104 38-39 -1.0314647736845046 1104 40-41 -1.1045559038327468 1104 42-43 -1.010274341497059 1104 44-45 -1.0705406620057047 1104 46-47 -1.0813863390879206 1104 48-49 -0.9166288826785305 1104 50-51 -0.8587858033093525 1104 52-53 -0.7768716143961214 1104 54-55 -0.7974030415967803 1104 56-57 -0.8490861153195439 1104 58-59 -0.8447432633033927 1104 60-61 -0.8729597272978111 1104 62-63 -0.8426069518361814 1104 64-65 -0.9176997950925667 1104 66-67 -0.8867518757884909 1104 68-69 -0.8457114058593405 1104 70-71 -0.8950536906443674 1104 72-73 -0.9650101435081666 1104 74-75 -1.0750325979663842 1104 76-77 -1.0225788826625148 1104 78-79 -1.02643262411382 1104 80-81 -1.2376890291024907 1104 82-83 -1.2526985808819049 1104 84-85 -1.3526137451004416 1104 86-87 -1.2189458209167867 1104 88-89 -1.371594577191427 1104 90-91 -1.396936674910485 1104 92-93 -1.464586212510806 1104 94-95 -1.470096654412611 1104 96-97 -1.5547974619827833 1104 98-99 -1.5215329050637258 1104 100 -1.4823812593064183 1105 1 -0.47430194380363133 1105 2 -0.5645774298455741 1105 3 -0.516774046604759 1105 4 -0.33115719070033833 1105 5 -0.3533899783017844 1105 6 -0.2862651161328955 1105 7 -0.31589954999078884 1105 8 -0.3989289529097064 1105 9 -0.4879786196025364 1105 10-11 -0.4864046995605449 1105 12-13 -0.5109680969492985 1105 14-15 -0.696862801690493 1105 16-17 -0.7792855544448329 1105 18-19 -0.7024490992855021 1105 20-21 -0.7697881903649773 1105 22-23 -0.721314383890622 1105 24-25 -0.6018652474283286 1105 26-27 -0.5453665966916361 1105 28-29 -0.6856223516347271 1105 30-31 -0.7831691845044091 1105 32-33 -0.8392798378172444 1105 34-35 -0.9114749775862876 1105 36-37 -0.9091286556990141 1105 38-39 -0.8216270447012093 1105 40-41 -0.850927937546551 1105 42-43 -0.903082166583367 1105 44-45 -0.9405255778766914 1105 46-47 -0.8898594986686774 1105 48-49 -0.9987141468840548 1105 50-51 -0.9426858281903918 1105 52-53 -0.8255393125891359 1105 54-55 -0.8703950641141205 1105 56-57 -0.9007706393141888 1105 58-59 -0.7777930564797657 1105 60-61 -0.8128160393060142 1105 62-63 -0.8367253078216308 1105 64-65 -0.8172772062206022 1105 66-67 -0.8665325338919345 1105 68-69 -0.8602866398966071 1105 70-71 -0.8864732315892212 1105 72-73 -0.988672787740434 1105 74-75 -0.9505838684543662 1105 76-77 -1.0016033904844903 1105 78-79 -1.0772023812126896 1105 80-81 -1.0607630812904674 1105 82-83 -0.9907241927999202 1105 84-85 -1.0060049527638029 1105 86-87 -1.041877584671333 1105 88-89 -1.1825597281882203 1105 90-91 -1.1982017201628707 1105 92-93 -1.1488594529549303 1105 94-95 -1.1384468083640336 1105 96-97 -1.2888584360754045 1105 98-99 -1.218720570289367 1105 100 -1.334179691801051 1106 1 -0.40381749630117625 1106 2 -0.4475300778168503 1106 3 -0.46076129399157395 1106 4 -0.35597770213215085 1106 5 -0.3837554316725189 1106 6 -0.33669087705229117 1106 7 -0.3747842722564485 1106 8 -0.404323717152657 1106 9 -0.4332154407707449 1106 10-11 -0.5473337455097678 1106 12-13 -0.5293400734670257 1106 14-15 -0.5695977406315862 1106 16-17 -0.6504595511815481 1106 18-19 -0.6396349656859002 1106 20-21 -0.5218311791356882 1106 22-23 -0.47246579390185417 1106 24-25 -0.5546905237362125 1106 26-27 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2208 50-51 0.8747146292670251 2208 52-53 0.8711699804301389 2208 54-55 0.8642231387844959 2208 56-57 0.8469216035610359 2208 58-59 0.9362851180019476 2208 60-61 0.8957975973553687 2208 62-63 0.8364367936002921 2208 64-65 0.9467971124465393 2208 66-67 0.8447950602822445 2208 68-69 0.8872310492110032 2208 70-71 0.9530363772793393 2208 72-73 0.9236026668883923 2208 74-75 1.0145519753169623 2208 76-77 0.9881562210637753 2208 78-79 1.0147646767323089 2208 80-81 1.005637023039835 2208 82-83 0.9576998955902383 2208 84-85 0.9885516494030782 2208 86-87 1.0124375184501204 2208 88-89 0.9804502665595329 2208 90-91 1.054181887831497 2208 92-93 1.1152053378306555 2208 94-95 1.1352968213539079 2208 96-97 1.1885222242306064 2208 98-99 1.0762020514025217 2208 100 1.299893233413414 2209 1 0.576188637686947 2209 2 0.39747089120274737 2209 3 0.5113094914787801 2209 4 0.42424248169933776 2209 5 0.4157140348022281 2209 6 0.4418954080276265 2209 7 0.4238006957094598 2209 8 0.4198638398830852 2209 9 0.5678633132393998 2209 10-11 0.6666092234533707 2209 12-13 0.6334409939597947 2209 14-15 0.7962756094479175 2209 16-17 0.7563487616894875 2209 18-19 0.7404403356039353 2209 20-21 0.8064935459167657 2209 22-23 0.7649685808923508 2209 24-25 0.7242621071990243 2209 26-27 0.7993639520389095 2209 28-29 0.7429886229762488 2209 30-31 0.8482255738903532 2209 32-33 0.8484400639026575 2209 34-35 0.9087125736012567 2209 36-37 0.9045187644484045 2209 38-39 0.8621035040293421 2209 40-41 0.9108714072527917 2209 42-43 0.8821299546287591 2209 44-45 0.8770694262183056 2209 46-47 0.8978497560405785 2209 48-49 0.940490603320697 2209 50-51 0.91757092856637 2209 52-53 0.8842588037089811 2209 54-55 0.861757679538627 2209 56-57 0.8044205973770531 2209 58-59 0.8738633726766665 2209 60-61 0.8867470237570458 2209 62-63 0.8546932015968807 2209 64-65 0.8780913441479399 2209 66-67 0.9089715611121676 2209 68-69 0.9179869063769388 2209 70-71 0.8512282426122582 2209 72-73 0.8595000214323747 2209 74-75 0.9537625688920741 2209 76-77 0.9163474694663734 2209 78-79 0.9375203870100783 2209 80-81 1.0170190644916772 2209 82-83 0.9669138088380791 2209 84-85 0.9712579464990938 2209 86-87 0.9540957978020472 2209 88-89 1.0178866272581324 2209 90-91 1.0808769039157546 2209 92-93 1.112369867274392 2209 94-95 1.1565007755835524 2209 96-97 1.220683742466779 2209 98-99 1.1704202978515 2209 100 1.2012052385838778 2210 1 0.6593084364881605 2210 2 0.6124218743661167 2210 3 0.6484919837055152 2210 4 0.5982230141223468 2210 5 0.5461664566528768 2210 6 0.5807849588817788 2210 7 0.6218980625855863 2210 8 0.6074146795401205 2210 9 0.6803831250866423 2210 10-11 0.7936446579224423 2210 12-13 0.7871350645388233 2210 14-15 1.000942203636633 2210 16-17 1.0133654533610397 2210 18-19 0.9946329385044947 2210 20-21 0.9471673009268002 2210 22-23 1.0074708675716195 2210 24-25 1.0727952839799855 2210 26-27 1.0508913668229667 2210 28-29 1.0427321090206192 2210 30-31 1.0549703427944124 2210 32-33 1.0605973120599117 2210 34-35 1.0986221066258324 2210 36-37 1.0529631122555898 2210 38-39 1.117896449025828 2210 40-41 1.1766834429409343 2210 42-43 1.0654877710131174 2210 44-45 1.1289132797258858 2210 46-47 1.1109314169452489 2210 48-49 1.2139225646464595 2210 50-51 1.1850416076122698 2210 52-53 1.0870210253030592 2210 54-55 1.009009330223904 2210 56-57 1.0557031816507774 2210 58-59 0.9888881963740488 2210 60-61 1.0258738674148589 2210 62-63 1.0477683974685377 2210 64-65 1.0561300908592628 2210 66-67 0.9783651091251784 2210 68-69 0.9993489409779173 2210 70-71 1.0425414125802916 2210 72-73 0.9826286625433198 2210 74-75 0.9912552957299354 2210 76-77 0.9405863857760792 2210 78-79 0.9650871428246255 2210 80-81 0.9506739389961076 2210 82-83 0.9332065762635935 2210 84-85 1.0793215241555956 2210 86-87 1.0777993629923408 2210 88-89 1.1346300480723492 2210 90-91 1.2045210951705627 2210 92-93 1.2193185601345853 2210 94-95 1.1785527132815083 2210 96-97 1.2968349604587033 2210 98-99 1.255282495527858 2210 100 1.3127702549276563 2211 1 0.5883176235071446 2211 2 0.5593446405729345 2211 3 0.6067014154692778 2211 4 0.5165951773014399 2211 5 0.5050966015624638 2211 6 0.5067718206095009 2211 7 0.49031678294763736 2211 8 0.4984596795145535 2211 9 0.6026819989084444 2211 10-11 0.6499396776389332 2211 12-13 0.7101315575529696 2211 14-15 0.8887231729691152 2211 16-17 0.8506029904377712 2211 18-19 0.8046061745156123 2211 20-21 0.8249438446283435 2211 22-23 0.8700062257494707 2211 24-25 0.8602074033913567 2211 26-27 0.8429594317769116 2211 28-29 0.8384451838342386 2211 30-31 0.8887773625609086 2211 32-33 0.9407924715899867 2211 34-35 0.9471605689946188 2211 36-37 0.9061089177630279 2211 38-39 0.8822085929230283 2211 40-41 0.8793694998173933 2211 42-43 0.9720311998601687 2211 44-45 0.9929034433492419 2211 46-47 1.0519493745535016 2211 48-49 1.024928665654251 2211 50-51 1.0025750313519453 2211 52-53 0.992956853086362 2211 54-55 0.8745038106838052 2211 56-57 0.925092302552315 2211 58-59 0.953062969595976 2211 60-61 0.9149243792598156 2211 62-63 0.8431243539757745 2211 64-65 0.9787564272833364 2211 66-67 0.9151851938768658 2211 68-69 0.8816717836532675 2211 70-71 0.8549410476219705 2211 72-73 0.9232622038263898 2211 74-75 0.8530387511421225 2211 76-77 0.982035657762637 2211 78-79 0.9316640564839069 2211 80-81 0.9662362242123983 2211 82-83 0.8889063230538738 2211 84-85 0.9307768409063542 2211 86-87 0.9348617950668583 2211 88-89 1.050830555855267 2211 90-91 1.1428224880228584 2211 92-93 1.158552261285763 2211 94-95 1.1280117523344337 2211 96-97 1.1502880036054783 2211 98-99 1.2793796298892168 2211 100 1.318275705725604 2212 1 0.5707213997163478 2212 2 0.4836231310378274 2212 3 0.5715184536334128 2212 4 0.4685519708325998 2212 5 0.3775482863026056 2212 6 0.4526374417852139 2212 7 0.5329361833933532 2212 8 0.5653799060473972 2212 9 0.667050063490926 2212 10-11 0.729661055917795 2212 12-13 0.8171961494149755 2212 14-15 0.9490642903127551 2212 16-17 0.902687758811858 2212 18-19 0.918733262551612 2212 20-21 0.9079876605011918 2212 22-23 0.9659627060304175 2212 24-25 0.9963089935222058 2212 26-27 1.0113600964409102 2212 28-29 1.0158268918971771 2212 30-31 0.9501351643385973 2212 32-33 0.9497218673093784 2212 34-35 0.9753281324870713 2212 36-37 1.1306852165243058 2212 38-39 1.1519870621552926 2212 40-41 1.1498533886643116 2212 42-43 1.1899165458720447 2212 44-45 1.3133341679002797 2212 46-47 1.2860624049349099 2212 48-49 1.3099674662147862 2212 50-51 1.289020534915437 2212 52-53 1.2137651323776026 2212 54-55 1.1715770996510528 2212 56-57 1.1488286716184106 2212 58-59 1.1207837489492576 2212 60-61 1.1478023600313279 2212 62-63 1.1164018225170196 2212 64-65 1.1211514788124433 2212 66-67 1.1000121644991765 2212 68-69 1.0990741287281551 2212 70-71 1.1328595827139907 2212 72-73 1.0887147318052044 2212 74-75 1.1295718434345758 2212 76-77 1.2070345961312086 2212 78-79 1.0880216569652639 2212 80-81 1.1336572926051822 2212 82-83 1.1742455239459737 2212 84-85 1.1516375399727252 2212 86-87 1.256316594109684 2212 88-89 1.2992515652378849 2212 90-91 1.2263048541869814 2212 92-93 1.415422009312369 2212 94-95 1.506111233541354 2212 96-97 1.4919599876208949 2212 98-99 1.5527058231767263 2212 100 1.607049987442327 2213 1 0.5446550742040159 2213 2 0.4980800204701019 2213 3 0.5470287089701493 2213 4 0.38785736470662613 2213 5 0.3880991517949397 2213 6 0.37157396575681645 2213 7 0.4068432733849434 2213 8 0.4638101383104001 2213 9 0.5395794620694758 2213 10-11 0.5296855564031304 2213 12-13 0.6126078524177956 2213 14-15 0.7127988161989691 2213 16-17 0.6798531728900983 2213 18-19 0.6110364230607814 2213 20-21 0.6348863345627223 2213 22-23 0.6961968125762752 2213 24-25 0.6271052218902469 2213 26-27 0.7396892301363422 2213 28-29 0.6920764634058614 2213 30-31 0.7443375800276755 2213 32-33 0.7593905817392539 2213 34-35 0.6998871037124985 2213 36-37 0.6699226769915541 2213 38-39 0.7036761929438029 2213 40-41 0.8484072722316967 2213 42-43 0.8902434950967262 2213 44-45 0.8853425370737042 2213 46-47 0.7943760314529342 2213 48-49 0.796475260014212 2213 50-51 0.7959322634340324 2213 52-53 0.6951939782390966 2213 54-55 0.6647373496575284 2213 56-57 0.7033879928507716 2213 58-59 0.7412895330142106 2213 60-61 0.6647416535997763 2213 62-63 0.7555662357357349 2213 64-65 0.7051569367831618 2213 66-67 0.7298476639123095 2213 68-69 0.7204709876964657 2213 70-71 0.7888070688493087 2213 72-73 0.88038686454707 2213 74-75 0.9894955953592728 2213 76-77 0.9874038561176945 2213 78-79 1.020302128475361 2213 80-81 0.9440140779170711 2213 82-83 1.0219993477717217 2213 84-85 1.0388851041515679 2213 86-87 1.1356715553778045 2213 88-89 1.1594900027855601 2213 90-91 1.1752152238236917 2213 92-93 1.1430518772475402 2213 94-95 1.1633469589360637 2213 96-97 1.31397411454196 2213 98-99 1.3408951731871355 2213 100 1.4999547591055524 2214 1 0.484388956075648 2214 2 0.4388930648017286 2214 3 0.499960266762276 2214 4 0.4153195752453058 2214 5 0.34350286034064936 2214 6 0.457502962395651 2214 7 0.3750522157700473 2214 8 0.4198696746716095 2214 9 0.48502569848972854 2214 10-11 0.5342840742525041 2214 12-13 0.6040686685300756 2214 14-15 0.6237434655137832 2214 16-17 0.7309619895477937 2214 18-19 0.7140523361930207 2214 20-21 0.7039245558541438 2214 22-23 0.751235003507766 2214 24-25 0.7991892635110531 2214 26-27 0.7649335943074718 2214 28-29 0.7354779317080897 2214 30-31 0.7954946263683809 2214 32-33 0.8573160395816899 2214 34-35 0.8347230229220557 2214 36-37 0.8860647870441838 2214 38-39 1.004581185261948 2214 40-41 0.9775250578527519 2214 42-43 1.045498759549055 2214 44-45 1.087925022215309 2214 46-47 1.0853944181207638 2214 48-49 1.0793511925501846 2214 50-51 1.0584601166751781 2214 52-53 1.0053235965108698 2214 54-55 0.8996671982386957 2214 56-57 0.9224242300939451 2214 58-59 0.9361674128469417 2214 60-61 0.8984717788291761 2214 62-63 0.9762196720407346 2214 64-65 0.9778552876795388 2214 66-67 0.9970809072932525 2214 68-69 0.9563718481342462 2214 70-71 0.9975673476174123 2214 72-73 1.0882787693372578 2214 74-75 1.090802563842736 2214 76-77 1.075674056325468 2214 78-79 1.1200355735900693 2214 80-81 1.189869933748934 2214 82-83 1.2287155242098038 2214 84-85 1.2356292410226715 2214 86-87 1.2263050366847423 2214 88-89 1.3875614359794604 2214 90-91 1.3880621093606216 2214 92-93 1.5666706534279875 2214 94-95 1.6522071005784689 2214 96-97 1.6825233278601708 2214 98-99 1.7043432253444344 2214 100 1.7169623935634348 2215 1 0.4096846648665178 2215 2 0.22864059913194978 2215 3 0.41507079346792963 2215 4 0.35181840366766437 2215 5 0.332826260129643 2215 6 0.3499152447310081 2215 7 0.4028608633898756 2215 8 0.4238997322607503 2215 9 0.4702551155299375 2215 10-11 0.5113598947230358 2215 12-13 0.5616728696790148 2215 14-15 0.7358487093437986 2215 16-17 0.6566327224544537 2215 18-19 0.631390278066533 2215 20-21 0.6508426883355227 2215 22-23 0.7958824117871828 2215 24-25 0.7644202802496096 2215 26-27 0.748675338964226 2215 28-29 0.7692032922731968 2215 30-31 0.7438156336196542 2215 32-33 0.8660320415452674 2215 34-35 0.8836060746150096 2215 36-37 0.9280313404799685 2215 38-39 1.029972839335386 2215 40-41 1.1093229388435617 2215 42-43 1.0133242118483352 2215 44-45 1.0960506580666376 2215 46-47 1.1045991943659672 2215 48-49 1.0632987845909057 2215 50-51 0.7123941243579139 2215 52-53 0.8651943892205125 2215 54-55 0.9742289656364846 2215 56-57 0.9500775374833594 2215 58-59 0.958873373224634 2215 60-61 0.9643820448540907 2215 62-63 0.8943731566629154 2215 64-65 0.8562830258839327 2215 66-67 0.8887680157124151 2215 68-69 0.8720553210845949 2215 70-71 0.9432374485961432 2215 72-73 1.0357583917540367 2215 74-75 1.0547594454332483 2215 76-77 1.001923829814885 2215 78-79 1.0475501968626695 2215 80-81 1.1182332132121537 2215 82-83 1.1977082907274976 2215 84-85 1.1922777558037154 2215 86-87 1.3805568915606123 2215 88-89 1.4303493649423906 2215 90-91 1.5309340303336398 2215 92-93 1.6456081817025776 2215 94-95 1.7666348103251828 2215 96-97 1.8188236953409067 2215 98-99 1.7560665524407923 2215 100 1.841759789518175 2216 1 0.299174283619962 2216 2 0.11671810013416817 2216 3 0.241234303508854 2216 4 0.23401418368156612 2216 5 0.16427088146433277 2216 6 0.17694758392190835 2216 7 0.20338237718587493 2216 8 0.2952650702316859 2216 9 0.3178127322414497 2216 10-11 0.330263814476389 2216 12-13 0.23947334757109928 2216 14-15 0.4359092389762793 2216 16-17 0.1687552324117476 2216 18-19 0.4331694310698708 2216 20-21 0.42138076396187785 2216 22-23 0.5713764186686063 2216 24-25 0.485716652653565 2216 26-27 0.4617827262471579 2216 28-29 0.5495832914656518 2216 30-31 0.4733173943505946 2216 32-33 0.5702436638115245 2216 34-35 0.5202377501790707 2216 36-37 0.5125424600510513 2216 38-39 0.6770636316210457 2216 40-41 0.6640981103742334 2216 42-43 0.5834751357634147 2216 44-45 0.5933455711260365 2216 46-47 0.5054569558583069 2216 48-49 0.5470305896501557 2216 50-51 0.59863176246931 2216 52-53 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0.4861636028846128 2311 4 0.3979802841267954 2311 5 0.34908787934848107 2311 6 0.2692031807147117 2311 7 0.3991158633004872 2311 8 0.43905715231777975 2311 9 0.5616445205378469 2311 10-11 0.5926055260013996 2311 12-13 0.6276411386427014 2311 14-15 0.7713248820144329 2311 16-17 0.7057007430414615 2311 18-19 0.6986809914997565 2311 20-21 0.7231047508038273 2311 22-23 0.8173724069513426 2311 24-25 0.8251990093773074 2311 26-27 0.9283297907978536 2311 28-29 0.9476374454181737 2311 30-31 0.9002416497684962 2311 32-33 0.8605471292511027 2311 34-35 0.8616099291193038 2311 36-37 0.7668211517163073 2311 38-39 0.8465774209170505 2311 40-41 0.8307916708971987 2311 42-43 0.7635443103879354 2311 44-45 0.7857732281634924 2311 46-47 0.8345969628567502 2311 48-49 0.8419676550046447 2311 50-51 0.8612285123618832 2311 52-53 0.7390698317613911 2311 54-55 0.6306977968925338 2311 56-57 0.6412668316025929 2311 58-59 0.6320371529194091 2311 60-61 0.6366960632233258 2311 62-63 0.6352163106717157 2311 64-65 0.6393790219873452 2311 66-67 0.6612191296355903 2311 68-69 0.6612300540338865 2311 70-71 0.6306659083947466 2311 72-73 0.5425782305795579 2311 74-75 0.6572773988896614 2311 76-77 0.7250583447979295 2311 78-79 0.7915398388226329 2311 80-81 0.871209885165257 2311 82-83 0.8574695519455489 2311 84-85 1.1305981924600346 2311 86-87 1.0263877357491609 2311 88-89 1.0682427079590404 2311 90-91 1.188627084769383 2311 92-93 1.2103639191994766 2311 94-95 1.3380234827614323 2311 96-97 1.3563564375877455 2311 98-99 1.400254234030264 2311 100 1.364481502549797 2312 1 0.5253356247994532 2312 2 0.5030966313629683 2312 3 0.5047865029730296 2312 4 0.38532555379965316 2312 5 0.3971748498492502 2312 6 0.4263968012703998 2312 7 0.5402804936892522 2312 8 0.5617060551390978 2312 9 0.7032445392930384 2312 10-11 0.6627281715550808 2312 12-13 0.6482207745240629 2312 14-15 0.8684792841257334 2312 16-17 0.862954648140196 2312 18-19 0.773051185482025 2312 20-21 0.8166002160665329 2312 22-23 0.8498883823552532 2312 24-25 0.874213692012674 2312 26-27 0.8832294170336894 2312 28-29 0.9615765714262636 2312 30-31 0.9915526376312087 2312 32-33 0.921113000161796 2312 34-35 0.9718159347994444 2312 36-37 1.016950930940915 2312 38-39 1.0398538523400376 2312 40-41 1.0524964507916295 2312 42-43 1.0200826714521654 2312 44-45 1.0328636616763376 2312 46-47 1.01496078007402 2312 48-49 1.0124950442820335 2312 50-51 1.0262683336517 2312 52-53 0.9614310433467423 2312 54-55 0.8991850567591015 2312 56-57 0.8711870550572982 2312 58-59 0.8076895656407856 2312 60-61 0.9527134988528303 2312 62-63 0.9203965279640016 2312 64-65 0.9982972828452645 2312 66-67 0.9737693667547944 2312 68-69 0.981963917871191 2312 70-71 0.9460746375965812 2312 72-73 1.0011100005337354 2312 74-75 0.9954703362205279 2312 76-77 1.1053868285746944 2312 78-79 1.1069904322904698 2312 80-81 1.243054156794006 2312 82-83 1.2055759474657393 2312 84-85 1.1703826417272403 2312 86-87 1.2893723564924002 2312 88-89 1.3144422698913623 2312 90-91 1.3336725326165535 2312 92-93 1.4393949456442954 2312 94-95 1.3854607198538424 2312 96-97 1.5010669713069014 2312 98-99 1.5493789359566925 2312 100 1.4649791849440312 2313 1 0.5321560232884011 2313 2 0.4883893977468965 2313 3 0.5441218452457441 2313 4 0.42698112692558254 2313 5 0.45222973934413346 2313 6 0.5250722773289667 2313 7 0.5376515486468776 2313 8 0.552841307587741 2313 9 0.6072599752266115 2313 10-11 0.6018933232250774 2313 12-13 0.6804137254865736 2313 14-15 0.849742349851482 2313 16-17 0.8293104043207578 2313 18-19 0.8203822252538231 2313 20-21 0.8358232491257382 2313 22-23 0.7944383274926494 2313 24-25 0.8386930710254035 2313 26-27 0.8989978766854776 2313 28-29 0.8815816094625433 2313 30-31 0.9255790147093919 2313 32-33 0.9864626954592097 2313 34-35 1.0422327448977526 2313 36-37 1.0855311437396793 2313 38-39 1.0392463409148078 2313 40-41 1.1561758981136734 2313 42-43 1.0118793342461814 2313 44-45 1.1102000397944138 2313 46-47 1.1394257688594536 2313 48-49 1.1512881813470983 2313 50-51 1.110612916059452 2313 52-53 1.0768960890566106 2313 54-55 0.9801947975602019 2313 56-57 1.0548917194541545 2313 58-59 1.0138413934080717 2313 60-61 1.0091282435355566 2313 62-63 1.0280518890800394 2313 64-65 1.0711236324025037 2313 66-67 1.0360085004635593 2313 68-69 1.0172144839779378 2313 70-71 0.9711379716291404 2313 72-73 1.0426164831955376 2313 74-75 1.1684903521055787 2313 76-77 1.155503635714723 2313 78-79 1.275910880621911 2313 80-81 1.2595592723730142 2313 82-83 1.3452539344744139 2313 84-85 1.417495407680871 2313 86-87 1.3513753882786474 2313 88-89 1.4526313132381965 2313 90-91 1.4577131654548694 2313 92-93 1.435293024915378 2313 94-95 1.5848723812045726 2313 96-97 1.7469904312545133 2313 98-99 1.6764033950849502 2313 100 1.7938148158729135 2314 1 0.41146978874981954 2314 2 0.2980103529847469 2314 3 0.4061042675981845 2314 4 0.33079754728051824 2314 5 0.2975758620297242 2314 6 0.366259270533277 2314 7 0.41538414211380115 2314 8 0.46012345091881457 2314 9 0.6591046067717414 2314 10-11 0.63479090029643 2314 12-13 0.5891103460629168 2314 14-15 0.7098587731747372 2314 16-17 0.6676966529151755 2314 18-19 0.6657531748169561 2314 20-21 0.6837135538317369 2314 22-23 0.7202925575997128 2314 24-25 0.7752491040330369 2314 26-27 0.785953136796941 2314 28-29 0.7564630406605559 2314 30-31 0.7701783667442612 2314 32-33 0.9195783780139237 2314 34-35 0.91653513692102 2314 36-37 0.8889656776098747 2314 38-39 0.8524780517453081 2314 40-41 0.8995949203690259 2314 42-43 0.8076963273108007 2314 44-45 0.9286121105240497 2314 46-47 0.7391147944847276 2314 48-49 0.6856232073460191 2314 50-51 0.8625359896247176 2314 52-53 0.7736638805972618 2314 54-55 0.8226054011578015 2314 56-57 0.8053631573046829 2314 58-59 0.7753315997232448 2314 60-61 0.5141618247520725 2314 62-63 0.6752090315380812 2314 64-65 0.78742903071511 2314 66-67 0.753506189108542 2314 68-69 0.796722291949088 2314 70-71 0.7725993416624561 2314 72-73 0.8242772998131969 2314 74-75 0.8866417242516391 2314 76-77 1.0137388364948663 2314 78-79 1.0408832884121573 2314 80-81 1.0523839446364107 2314 82-83 1.1171687556947063 2314 84-85 1.2046237029441436 2314 86-87 1.277912057413083 2314 88-89 1.0696881311025486 2314 90-91 1.1200763611749132 2314 92-93 1.3930279122766507 2314 94-95 1.395897937501239 2314 96-97 1.4647734925798481 2314 98-99 1.453337501605457 2314 100 1.352424144056517 2315 1 0.3955843817278577 2315 2 0.29763000091024594 2315 3 0.3568046414900543 2315 4 0.3708405717813008 2315 5 0.34123144212608736 2315 6 0.34666298453714006 2315 7 0.386319982873907 2315 8 0.3791070912553707 2315 9 0.31046411029181087 2315 10-11 0.4119269165130035 2315 12-13 0.39043896373196674 2315 14-15 0.5511618414954356 2315 16-17 0.42586767288580774 2315 18-19 0.5937081000975013 2315 20-21 0.4845024187643432 2315 22-23 0.4082037113532877 2315 24-25 0.5888447073945287 2315 26-27 0.7158987290898224 2315 28-29 0.5548544775517854 2315 30-31 0.5607113246986799 2315 32-33 0.6473148665193946 2315 34-35 0.5365751196573498 2315 36-37 0.7267727685088587 2315 38-39 0.7739209339112918 2315 40-41 0.8678374949285583 2315 42-43 0.8634416321985015 2315 44-45 0.8407566207442159 2315 46-47 0.8630357098716885 2315 48-49 0.7056506905453048 2315 50-51 0.7100076531321236 2315 52-53 0.5178294481183343 2315 54-55 0.6263923137216381 2315 56-57 0.5349910162055309 2315 58-59 0.6008097596230471 2315 60-61 0.725353234750358 2315 62-63 0.7326789148083961 2315 64-65 0.7844391898506302 2315 66-67 0.5934943071832919 2315 68-69 0.6689931474477007 2315 70-71 0.6289390479359653 2315 72-73 0.8096815388397047 2315 74-75 0.5777562526276938 2315 76-77 0.8886317013635079 2315 78-79 0.9535730225465819 2315 80-81 1.0090359078633604 2315 82-83 1.0589249374943712 2315 84-85 1.12160218290785 2315 86-87 1.1609817290750755 2315 88-89 1.0741855971054903 2315 90-91 1.2855374047052877 2315 92-93 1.4977383912235602 2315 94-95 1.5448033547248414 2315 96-97 1.6973102557384507 2315 98-99 1.8772099812863452 2315 100 1.8532452415916225 2316 1 0.23972733704954763 2316 2 0.29058297641546105 2316 3 0.28085992241285496 2316 4 0.18623591710157683 2316 5 0.2010074723220754 2316 6 0.0016651812313597247 2316 7 0.030540801166544895 2316 8 0.052160644406306744 2316 9 0.1353769400737832 2316 10-11 0.21212645022846033 2316 12-13 0.26861097468952266 2316 14-15 0.03332035459329319 2316 16-17 0.2488389465570222 2316 18-19 0.3444090796481447 2316 20-21 0.3615764504336312 2316 22-23 0.31940747719287543 2316 24-25 0.2797365158558236 2316 26-27 0.21921216159781665 2316 28-29 0.18351565429573213 2316 30-31 -0.46805683087004013 2316 32-33 -0.24401547682961677 2316 34-35 -0.2899747609602912 2316 36-37 -0.0436672466055299 2316 38-39 -0.04008356583068462 2316 40-41 0.18552325063419772 2316 42-43 0.2041325381156227 2316 44-45 0.0665354067786339 2316 46-47 -0.02162770197775643 2316 48-49 0.12151950872695494 2316 50-51 0.2684641797363483 2316 52-53 -0.10799683443345032 2316 54-55 0.016189884184662162 2316 56-57 -0.020681323297203846 2316 58-59 -0.35610253376722767 2316 60-61 0.19365780875588 2316 62-63 0.17119499097917412 2316 64-65 -0.08320844620845236 2316 66-67 -0.4861126986765072 2316 68-69 -0.5364682531851344 2316 70-71 -0.33590404462066203 2316 72-73 -0.502507305505766 2316 74-75 -0.27701514535733907 2316 76-77 0.11609793004701885 2316 78-79 0.32600625194877253 2316 80-81 0.37884834259768496 2316 82-83 0.619187319627315 2316 84-85 0.7330658790743243 2316 86-87 0.9173182106093662 2316 88-89 0.9537852359869312 2316 90-91 0.3451138223665069 2316 92-93 0.3951458874340581 2316 94-95 0.9172968283544485 2316 96-97 1.3221663251065898 2316 98-99 1.4379317223692105 2316 100 1.300054528124992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 96.0 1 261.0 2 160.0 3 124.0 4 137.0 5 146.0 6 164.0 7 286.0 8 787.0 9 1481.0 10 2139.0 11 2585.0 12 3267.0 13 3739.0 14 4583.0 15 6507.0 16 9324.0 17 12700.0 18 17458.0 19 23142.0 20 29819.0 21 36197.0 22 38030.0 23 37978.0 24 38116.0 25 41681.0 26 47243.0 27 55656.0 28 66547.0 29 80733.0 30 98860.0 31 121541.0 32 153006.0 33 196027.0 34 261334.0 35 364041.0 36 520259.0 37 688452.0 38 533828.0 39 96448.0 40 21.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.031950161865424 19.90253294695238 14.118309921856632 23.947206969325563 2 16.920570362311558 21.15164579104815 37.98252446054804 23.945259386092246 3 19.63738868886634 24.61179690049598 29.580696416036762 26.170117994600922 4 14.573544071466745 17.12548274525657 35.81369545615027 32.487277727126404 5 15.12589564262715 35.96164605989583 33.389145366418795 15.523312931058229 6 31.715266825684797 35.24188482957592 17.549851793657144 15.49299655108214 7 27.383765506397367 31.93693775177241 22.04626698485365 18.633029756976573 8 25.358760336213027 33.51221840211156 21.053413680881466 20.07560758079395 9 25.857607159155414 16.86049553109313 21.55671560388678 35.72518170586467 10-11 25.25219751047136 25.799084597892037 27.1842786589092 21.764439232727405 12-13 25.52658604004543 23.850445933215134 27.453112697461503 23.169855329277933 14-15 23.336515695090974 25.676539408370104 25.44302399126042 25.5439209052785 16-17 22.54296655313922 28.06274350913623 26.557686255183242 22.83660368254131 18-19 22.258012945602506 27.21844858971239 28.75403619598364 21.769502268701466 20-21 22.950825831258133 26.61231071337527 28.484568749881724 21.952294705484874 22-23 23.276797133286404 26.13581573305566 28.157471098187475 22.42991603547046 24-25 22.948532398926062 26.261165198664305 28.29452255836717 22.495779844042467 26-27 22.66950081293368 26.572333980405254 28.261076195328418 22.49708901133264 28-29 22.48318439939751 26.719410468755044 28.30699854053278 22.490406591314667 30-31 22.82925724183919 26.562891369907387 28.190906692404543 22.416944695848876 32-33 22.745342662386925 26.709833298175916 27.88573764624883 22.65908639318833 34-35 22.898828897537335 26.40310564857297 28.151389653617326 22.546675800272368 36-37 22.86189391687844 25.966582848288738 28.192389335808926 22.9791338990239 38-39 22.624904596550376 25.975341556525112 28.42478033725337 22.974973509671145 40-41 22.567569824239133 25.646432597169454 28.795836290513364 22.990161288078042 42-43 22.31640603799422 25.775087916079283 28.52029619403743 23.388209851889066 44-45 21.907071409933028 25.987161868313475 28.674569018004696 23.431197703748804 46-47 21.904732745387566 25.894631805574303 28.513291406129316 23.687344042908816 48-49 21.961617472053824 25.80700963564307 28.568595488503316 23.662777403799787 50-51 21.70454327223479 26.042802285260063 28.403863800637865 23.848790641867286 52-53 21.55682405721201 26.29879036158328 28.281767882753517 23.862617698451192 54-55 21.446493698817633 26.212899156173275 28.211607512241187 24.128999632767904 56-57 21.65488589328405 25.92912665580367 28.010811787202677 24.40517566370961 58-59 21.442034110204812 25.75439027153068 28.122828243020248 24.68074737524426 60-61 21.65560229431671 25.57429234239305 28.03059831103134 24.739507052258894 62-63 21.77598122310601 25.328169112282144 28.038675436305194 24.857174228306647 64-65 21.617404305570904 25.419311528363835 27.888325350621894 25.074958815443367 66-67 22.074977713864104 25.202161622981755 27.488428325755258 25.234432337398875 68-69 21.870158325074986 25.27427199091969 27.50471459889761 25.35085508510771 70-71 21.96003559343876 25.0675170347782 27.443437522353754 25.52900984942929 72-73 22.308224148138375 25.006575744645676 27.3728696326963 25.31233047451965 74-75 22.403353458201586 25.050799219719533 27.47395191907081 25.07189540300807 76-77 22.44449302602991 25.143447490627363 27.315007252216404 25.09705223112632 78-79 22.791900750627377 25.064463549703735 27.147351538997665 24.996284160671223 80-81 22.792805443611382 24.879265207657163 27.353888320682408 24.974041028049047 82-83 22.844439323430574 24.533622447964305 27.651206631935615 24.970731596669506 84-85 23.09686123043018 24.48514977423159 27.32128975370744 25.096699241630787 86-87 23.258073242974096 24.38107410188599 27.409963155296058 24.95088949984386 88-89 23.686242112094444 23.935434585131762 27.290897175016067 25.087426127757734 90-91 24.141305945387415 23.788731422056145 27.006593305036812 25.06336932751963 92-93 24.41768537277768 23.42322555286276 27.025372588384826 25.133716485974734 94-95 24.391850040296035 23.10691463226216 27.086019155578878 25.41521617186293 96-97 24.836042471190257 22.885393621248678 26.79930156507603 25.479262342485036 98-99 25.138568944270222 22.827005517423586 26.578483286885007 25.455942251421188 100 25.983637034612894 22.503631185723137 26.21352625385574 25.299205525808226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4805.0 1 3324.0 2 1967.5 3 2051.5 4 1871.5 5 1876.0 6 2676.0 7 4300.0 8 5769.0 9 5857.5 10 4757.0 11 3873.0 12 3748.0 13 3887.5 14 4041.0 15 4199.5 16 4445.5 17 4731.5 18 5108.5 19 5585.0 20 5989.5 21 6448.5 22 7070.0 23 7924.0 24 8819.0 25 9910.0 26 11464.0 27 12953.5 28 14362.0 29 16401.5 30 19328.5 31 22609.0 32 26655.5 33 32373.5 34 39662.0 35 47241.5 36 56282.5 37 67189.0 38 76077.5 39 83039.0 40 87629.0 41 92392.5 42 100295.5 43 110229.5 44 121877.5 45 135113.0 46 147217.0 47 157396.5 48 165223.5 49 169190.0 50 169152.5 51 166962.0 52 164663.0 53 159786.5 54 152590.0 55 141930.0 56 128518.0 57 110948.0 58 93620.5 59 80359.0 60 66436.0 61 52855.5 62 41710.5 63 32703.5 64 24566.5 65 17795.0 66 12841.5 67 9788.0 68 7752.0 69 5936.5 70 4550.5 71 3544.5 72 2750.0 73 2161.0 74 1723.0 75 1331.0 76 961.0 77 645.5 78 443.5 79 309.5 80 212.5 81 132.0 82 93.5 83 61.5 84 45.5 85 38.5 86 22.5 87 19.0 88 19.0 89 17.5 90 19.5 91 15.5 92 6.0 93 6.0 94 7.5 95 4.5 96 3.5 97 2.5 98 3.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02414529682720229 2 0.10325730624720611 3 0.01630085707458588 4 0.0171353719418855 5 0.019360744921351147 6 0.044563093913799624 7 0.04528634013212596 8 0.03749753470399619 9 0.03290770293384829 10-11 0.035911956456126916 12-13 0.03503571584546231 14-15 0.03160029630841222 16-17 0.03760880335296947 18-19 0.036927282878008116 20-21 0.046593746757562025 22-23 0.04616258074279056 24-25 0.06460535931011212 26-27 0.057066908342172235 28-29 0.05037688082265363 30-31 0.048819119737027676 32-33 0.042866247016957064 34-35 0.0439372077633249 36-37 0.03947255322327195 38-39 0.04479953979286785 40-41 0.05432691786120516 42-43 0.055300518539721386 44-45 0.058151777669661744 46-47 0.060307607743519086 48-49 0.05976517307977433 50-51 0.05463290664588169 52-53 0.06399338174075907 54-55 0.050279520754802014 56-57 0.05486935252494991 58-59 0.0529082425867958 60-61 0.04905556561609589 62-63 0.054980621173923196 64-65 0.053687123129608785 66-67 0.05179555609706298 68-69 0.057678885911525285 70-71 0.05858294368443322 72-73 0.05991816747211261 74-75 0.05107230987873664 76-77 0.051837281840427955 78-79 0.060502327879222335 80-81 0.0616011057878335 82-83 0.05186509900267128 84-85 0.057358988545727095 86-87 0.05521706705299142 88-89 0.06393774741627242 90-91 0.06873620790324524 92-93 0.07235243899487692 94-95 0.07616339022221183 96-97 0.07192127298010545 98-99 0.08118438800713121 100 0.1245652525255897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3594903.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.05609050881367 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25261368425181 45.19377778446216 2 14.553400608834352 17.480406883503598 3 4.582527269699475 8.256260174045353 4 1.9343250275148927 4.6467199570359154 5 1.0513937027618243 3.157129768673043 6 0.6238972906295899 2.2481299292552563 7 0.40835093997543387 1.7166772707366658 8 0.30553279352372054 1.4679284081016992 9 0.2157175446815053 1.1659637148958384 >10 1.027094501475799 10.28820542446995 >50 0.03416822349416076 1.3789463829864665 >100 0.01008565843833413 0.9815514555300617 >500 3.150899027810909E-4 0.11675532763737037 >1k 3.150898997895921E-4 0.40416518421227554 >5k 1.5754494989479606E-4 0.6405458362349837 >10k+ 1.050299665965307E-4 0.8568364982192042 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16079 0.4472721517103521 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11089 0.30846451211618225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8410 0.23394233446632634 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6859 0.1907979158269361 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5041 0.14022631486857923 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.563432448664123E-5 5 0.0 0.0 0.0 0.0 5.563432448664123E-5 6 0.0 0.0 0.0 0.0 1.6690297345992367E-4 7 0.0 0.0 0.0 0.0 1.6690297345992367E-4 8 0.0 0.0 0.0 0.0 1.6690297345992367E-4 9 0.0 0.0 0.0 2.7817162243320614E-5 2.225372979465649E-4 10-11 0.0 0.0 0.0 2.7817162243320614E-5 2.225372979465649E-4 12-13 0.0 0.0 0.0 4.1725743364980924E-5 2.225372979465649E-4 14-15 0.0 0.0 0.0 5.563432448664123E-5 3.0598878467652676E-4 16-17 0.0 0.0 0.0 1.1126864897328245E-4 3.0598878467652676E-4 18-19 0.0 0.0 0.0 1.1126864897328245E-4 3.3380594691984734E-4 20-21 0.0 0.0 0.0 4.311660147714695E-4 3.4771452804150765E-4 22-23 0.0 0.0 0.0 0.002308824466195611 4.0334885252814887E-4 24-25 0.0 0.0 0.0 0.006453581640450382 4.450745958931298E-4 26-27 0.0 0.0 0.0 0.01723273200973712 4.728917581364504E-4 28-29 0.0 0.0 0.0 0.047163998583550096 4.728917581364504E-4 30-31 0.0 0.0 0.0 0.16547038960439267 4.8680033925811066E-4 32-33 0.0 0.0 0.0 0.48184888437879964 5.563432448664122E-4 34-35 0.0 0.0 0.0 0.9834757711126003 6.537033127180344E-4 36-37 0.0 0.0 0.0 1.7068193495067878 6.676118938396947E-4 38-39 0.0 0.0 0.0 2.5967460039951007 6.676118938396947E-4 40-41 0.0 0.0 0.0 3.4880496080144585 6.676118938396947E-4 42-43 0.0 0.0 0.0 4.393233975993233 6.676118938396947E-4 44-45 0.0 0.0 0.0 5.502707583486954 6.81520474961355E-4 46-47 0.0 0.0 0.0 6.688497575595225 7.371547994479961E-4 48-49 0.0 0.0 0.0 7.7657728177923016 8.901491917862596E-4 50-51 0.0 0.0 0.0 8.787302466853765 8.901491917862596E-4 52-53 0.0 0.0 0.0 9.763768313081048 9.457835162729008E-4 54-55 0.0 0.0 0.0 10.841850252983182 9.736006785162214E-4 56-57 0.0 0.0 0.0 12.060005513361556 9.736006785162214E-4 58-59 0.0 0.0 0.0 13.292403160808512 9.736006785162214E-4 60-61 0.0 0.0 0.0 14.494994718911748 9.736006785162214E-4 62-63 0.0 0.0 0.0 15.697182928162455 9.736006785162214E-4 64-65 0.0 0.0 0.0 16.97565970486547 9.875092596378816E-4 66-67 0.0 0.0 0.0 18.363332751954644 0.001001417840759542 68-69 0.0 0.0 0.0 19.808740319279824 0.001001417840759542 70-71 0.0 0.0 0.0 21.3107140860268 0.0010292350030028626 72-73 0.0 0.0 0.0 22.785733022559995 0.0011126864897328245 74-75 0.0 0.0 0.0 24.262782611936956 0.0011126864897328245 76-77 0.0 0.0 0.0 25.800974323924734 0.0011265950708544848 78-79 0.0 0.0 0.0 27.383951667124258 0.0011405036519761451 80-81 0.0 0.0 0.0 28.994148103578873 0.0011544122330978054 82-83 0.0 2.7817162243320614E-5 0.0 30.56259097950626 0.0011683208142194658 84-85 0.0 2.7817162243320614E-5 0.0 32.11090813855061 0.0011683208142194658 86-87 0.0 5.563432448664123E-5 0.0 33.67869731116528 0.0011683208142194658 88 0.0 5.563432448664123E-5 0.0 34.849285224107575 0.0011683208142194658 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5465 0.0 49.994072 1 GTGGTAT 2290 0.0 42.507683 1 GTATCAA 10340 0.0 42.068176 1 TATCAAC 13250 0.0 32.579258 2 ATCAACG 13220 0.0 32.27097 3 TGGTATC 3015 0.0 32.128784 2 CAACGCA 13335 0.0 31.851284 5 TCAACGC 14275 0.0 29.952211 4 AACGCAG 14565 0.0 29.41954 6 ACGCAGA 16475 0.0 25.810287 7 CGCAGAG 17075 0.0 24.764715 8 GCAGAGT 17955 0.0 23.056664 9 CGTATCA 1185 0.0 22.224833 94 GTACATG 10620 0.0 21.254417 1 TACATGG 10990 0.0 20.195688 2 GAGTACT 11110 0.0 20.069529 12-13 CCGTATC 1475 0.0 19.768263 94 TATACCG 1030 0.0 19.61475 5 ATACCGA 1105 0.0 19.559021 6 TAGTACT 985 0.0 19.557405 4 >>END_MODULE