FastQCFastQC Report
Mon 6 Jun 2016
ERR1211365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594903
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140210.3900244318135983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99230.27602970094047047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78840.2193105071263397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66100.18387144242834924No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45130.12553885320410593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.050.5231821
GTATCAA106350.043.9480671
GTGGTAT22700.039.897841
ATCAACG136550.033.535873
TATCAAC138300.033.5148962
CAACGCA141050.032.459185
TGGTATC29100.031.2760472
TCAACGC147750.031.0889914
AACGCAG154300.030.0062716
ACGCAGA173000.026.356097
CGCAGAG180400.025.1449498
GCAGAGT191050.023.2508899
GTACATG111800.021.4904791
TACATGG116550.020.4884262
GAGTACT119000.020.27751212-13
ACATGGG119300.019.428443
CAGAGTA184850.019.18185610-11
CATGGGG78650.018.9108894
AGTACTT126550.018.82685712-13
AGAGTAC180250.018.65658810-11
Produced by FastQC (version 0.11.5)