##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211365_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3594903 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.974556476210903 31.0 31.0 34.0 30.0 34.0 2 32.11176156908824 33.0 31.0 34.0 30.0 34.0 3 32.17626539575616 34.0 31.0 34.0 30.0 34.0 4 35.85243523956001 37.0 35.0 37.0 35.0 37.0 5 35.72576506236747 37.0 35.0 37.0 33.0 37.0 6 35.73209541397918 37.0 35.0 37.0 33.0 37.0 7 35.63831040781907 37.0 35.0 37.0 33.0 37.0 8 35.62303906391911 37.0 35.0 37.0 33.0 37.0 9 37.21088023793688 39.0 37.0 39.0 33.0 39.0 10-11 37.16663662413144 39.0 37.0 39.0 33.0 39.0 12-13 37.09823867292108 39.0 37.0 39.0 33.0 39.0 14-15 38.29418735359479 40.0 38.0 41.0 33.0 41.0 16-17 38.272703463765225 40.0 38.0 41.0 33.0 41.0 18-19 38.262032243985445 40.0 38.0 41.0 33.0 41.0 20-21 38.222490982371426 40.0 38.0 41.0 33.0 41.0 22-23 38.10368569054575 40.0 37.0 41.0 33.0 41.0 24-25 37.99748866659267 40.0 37.0 41.0 33.0 41.0 26-27 37.869131378510076 40.0 37.0 41.0 33.0 41.0 28-29 37.731966620517994 40.0 37.0 41.0 32.5 41.0 30-31 37.72435264595457 40.0 37.0 41.0 33.0 41.0 32-33 37.64601311913006 40.0 36.5 41.0 32.5 41.0 34-35 37.49458344216798 40.0 36.0 41.0 32.0 41.0 36-37 37.323109135350805 40.0 36.0 41.0 31.5 41.0 38-39 37.1895215253374 39.0 36.0 41.0 31.0 41.0 40-41 37.045293572594304 39.0 35.0 41.0 31.0 41.0 42-43 36.89648761037502 39.0 35.0 41.0 31.0 41.0 44-45 36.6997700633369 39.0 35.0 41.0 30.0 41.0 46-47 36.430381848967826 39.0 35.0 41.0 30.0 41.0 48-49 34.822100206876236 38.0 34.0 40.0 22.0 41.0 50-51 35.5312666294473 38.0 34.0 40.0 27.0 41.0 52-53 35.61557057311421 38.0 34.5 40.0 28.0 41.0 54-55 35.4132025259096 38.0 34.0 40.0 27.0 41.0 56-57 35.161469029901504 38.0 34.0 40.0 26.5 41.0 58-59 34.88003862691149 37.0 34.0 40.0 26.0 41.0 60-61 34.595610924689765 37.0 33.5 40.0 26.0 41.0 62-63 34.24435360286495 36.0 33.0 39.0 26.0 41.0 64-65 33.87624339238083 35.5 33.0 39.0 25.0 40.5 66-67 33.50011488488006 35.0 32.5 38.5 24.5 40.0 68-69 33.095883115622314 35.0 32.0 38.0 23.5 40.0 70-71 32.71563238284872 35.0 32.0 37.0 23.5 39.5 72-73 32.32515161048852 35.0 31.0 36.5 23.0 39.0 74-75 31.95433145205865 35.0 31.0 36.0 22.0 39.0 76-77 30.957824592207356 33.5 29.5 35.0 20.0 37.0 78-79 31.394392004457423 34.0 31.0 35.0 21.0 37.0 80-81 31.26462716796531 34.0 31.0 35.0 20.0 37.0 82-83 31.00917563005177 34.0 30.0 35.0 20.0 36.0 84-85 30.75973065198143 34.0 30.0 35.0 19.5 36.0 86-87 30.505456336374028 34.0 30.0 35.0 19.0 35.0 88-89 30.276144585820536 34.0 29.0 35.0 18.0 35.0 90-91 30.066671339949924 34.0 29.0 35.0 18.0 35.0 92-93 29.88622238207818 34.0 29.0 35.0 18.0 35.0 94-95 29.706682767240174 33.0 29.0 35.0 17.0 35.0 96-97 29.51964851346476 33.0 29.0 35.0 16.5 35.0 98-99 29.26637742381366 33.0 27.5 35.0 15.0 35.0 100 26.628189689680084 30.0 23.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2931328520169174 1101 2 -0.2707707147948568 1101 3 -0.27336916996974736 1101 4 -0.21428648617666113 1101 5 -0.19945383508699877 1101 6 -0.2761165831154031 1101 7 -0.2938764616946088 1101 8 -0.18108225502307818 1101 9 -0.37746180138917396 1101 10-11 -0.3826775604788253 1101 12-13 -0.4561826071521651 1101 14-15 -0.5449794605807057 1101 16-17 -0.5283451779967194 1101 18-19 -0.6357905479245716 1101 20-21 -0.48229622927558324 1101 22-23 -0.7016500317090362 1101 24-25 -0.6150236714883164 1101 26-27 -0.7211829037754782 1101 28-29 -0.7354777603875036 1101 30-31 -0.6391307371048285 1101 32-33 -0.5792564369638455 1101 34-35 -0.673668548618771 1101 36-37 -0.5557665471228646 1101 38-39 -0.5627621873597022 1101 40-41 -0.6121454651255718 1101 42-43 -0.6010162662805811 1101 44-45 -0.5172019555336291 1101 46-47 -0.5977964383789143 1101 48-49 0.6730146287260013 1101 50-51 -0.23686407533662646 1101 52-53 -0.5864035791455251 1101 54-55 -0.6139329344642732 1101 56-57 -0.665471858500986 1101 58-59 -0.8179872821802334 1101 60-61 -0.7987517442267489 1101 62-63 -0.8449122922644392 1101 64-65 -0.9995252534279331 1101 66-67 -0.9222382954262827 1101 68-69 -0.9054882113268476 1101 70-71 -0.8412652213692517 1101 72-73 -0.9571079120857959 1101 74-75 -1.0528309658435049 1101 76-77 -1.0663204058378888 1101 78-79 -0.8776867511124493 1101 80-81 -0.7104756788675424 1101 82-83 -0.8631179067472949 1101 84-85 -1.015538123372938 1101 86-87 -0.9721511561810026 1101 88-89 -0.9657014127194259 1101 90-91 -1.075042883378181 1101 92-93 -1.081861913605227 1101 94-95 -1.0133564457799658 1101 96-97 -1.285892826041696 1101 98-99 -1.5473363202069308 1101 100 -1.3528952775281518 1102 1 -0.24584755885578602 1102 2 -0.2247479472726468 1102 3 -0.27780154359442477 1102 4 -0.22246447663270175 1102 5 -0.17149543775698817 1102 6 -0.3251005050571649 1102 7 -0.25179160290589664 1102 8 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68-69 -0.8384453161589605 1105 70-71 -0.7980799906840232 1105 72-73 -0.6991781072809893 1105 74-75 -0.9473334228459649 1105 76-77 -0.8124685426110219 1105 78-79 -0.7003218862475826 1105 80-81 -0.6179115238033894 1105 82-83 -0.5369465649509557 1105 84-85 -0.6217992140090267 1105 86-87 -0.6249204027002477 1105 88-89 -0.7559007029187157 1105 90-91 -0.8177145322998314 1105 92-93 -0.7913341699524814 1105 94-95 -0.7209646221381405 1105 96-97 -0.8502441091429809 1105 98-99 -0.9156691416647504 1105 100 -0.9144227140555863 1106 1 -0.27536337676141187 1106 2 -0.2550552122060985 1106 3 -0.3023430323125176 1106 4 -0.19661334448341705 1106 5 -0.2391468887217627 1106 6 -0.3257293476750007 1106 7 -0.37122241423460167 1106 8 -0.3537980422025413 1106 9 -0.40419539391173487 1106 10-11 -0.48860474014571764 1106 12-13 -0.4188790276596279 1106 14-15 -0.5550720427200133 1106 16-17 -0.5329189642134367 1106 18-19 -0.5617940731645561 1106 20-21 -0.622236310387855 1106 22-23 -0.5794943707843601 1106 24-25 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2209 74-75 1.1967902462777076 2209 76-77 1.1011985335560546 2209 78-79 1.0339496125239123 2209 80-81 0.9267919979001285 2209 82-83 0.9843278003234097 2209 84-85 0.9925136443038305 2209 86-87 1.0718715710917266 2209 88-89 1.0433793953613808 2209 90-91 1.1087576216723214 2209 92-93 1.164149450031136 2209 94-95 1.0520349683614505 2209 96-97 1.2176248507259793 2209 98-99 1.36339474039913 2209 100 1.4704986618657898 2210 1 0.5776010657302741 2210 2 0.5165677348776612 2210 3 0.5222740446447034 2210 4 0.3895963840225818 2210 5 0.4324009933939337 2210 6 0.4723089089494721 2210 7 0.5213748314327873 2210 8 0.5470784781155302 2210 9 0.6830892212746775 2210 10-11 0.6829452953818631 2210 12-13 0.6459070000535974 2210 14-15 0.8626019442352089 2210 16-17 0.816637815354639 2210 18-19 0.8475961095494782 2210 20-21 0.8625128924970298 2210 22-23 0.9017237563992637 2210 24-25 0.8630169445500897 2210 26-27 0.9063817963832435 2210 28-29 0.9213672005227309 2210 30-31 1.042568346834301 2210 32-33 0.9128048872324399 2210 34-35 1.0188935453537127 2210 36-37 1.0627233476391567 2210 38-39 1.044913129248144 2210 40-41 1.0657794428878375 2210 42-43 1.0638574016351257 2210 44-45 1.0981412260429622 2210 46-47 1.0553354998747082 2210 48-49 2.335563641938734 2210 50-51 1.4649251032224697 2210 52-53 1.1869383216853109 2210 54-55 1.1655977149502235 2210 56-57 1.0805292084348181 2210 58-59 1.0744121818453394 2210 60-61 1.055302090883501 2210 62-63 1.1096577368354517 2210 64-65 1.0053816924358685 2210 66-67 0.9694770069637997 2210 68-69 0.9715107078457379 2210 70-71 1.0787817866202403 2210 72-73 1.0388535184718748 2210 74-75 1.1042880900587306 2210 76-77 1.1478402678084088 2210 78-79 1.029523355212703 2210 80-81 0.9876770696170603 2210 82-83 1.016292112181528 2210 84-85 1.0431134863019018 2210 86-87 1.1523974420954737 2210 88-89 1.151263198714215 2210 90-91 1.2341475167967317 2210 92-93 1.2598674238376084 2210 94-95 1.219587605471613 2210 96-97 1.229358421786987 2210 98-99 1.3026425932399022 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1.117532853859501 2211 60-61 1.1615726247600264 2211 62-63 1.0720092239667522 2211 64-65 1.114373307598008 2211 66-67 1.1466922511319382 2211 68-69 1.1841088429450863 2211 70-71 1.1121054809028763 2211 72-73 1.1937608557589172 2211 74-75 1.1179987685551112 2211 76-77 0.8348737656873446 2211 78-79 0.8653924974299798 2211 80-81 0.8992879311039026 2211 82-83 1.0119454494992546 2211 84-85 1.0553144792566869 2211 86-87 1.0747821015747014 2211 88-89 1.0781977931156455 2211 90-91 1.1692139665942278 2211 92-93 1.108757238125861 2211 94-95 1.1425006705136163 2211 96-97 1.1802539445142166 2211 98-99 1.1850570163654481 2211 100 1.523294170395264 2212 1 0.5679980471770172 2212 2 0.5246161847321034 2212 3 0.4887514704957354 2212 4 0.35088732354817154 2212 5 0.40304205778647884 2212 6 0.3054847066571824 2212 7 0.25495994040392134 2212 8 0.4110661561844182 2212 9 0.5343227055093891 2212 10-11 0.5367578620656346 2212 12-13 0.6323080502343288 2212 14-15 0.7337359791661981 2212 16-17 0.7201711188819857 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0.9367760782556083 2212 86-87 0.9712982408541286 2212 88-89 1.0648739602346318 2212 90-91 1.1082245922839427 2212 92-93 1.1688255024053547 2212 94-95 1.1499853099477342 2212 96-97 1.1461861920237943 2212 98-99 1.2855817931884594 2212 100 1.3830349558480997 2213 1 0.4141088689906276 2213 2 0.38345908318494537 2213 3 0.394349951625955 2213 4 0.24865703127635186 2213 5 0.3088771008546942 2213 6 0.39490934067951144 2213 7 0.4378969688129999 2213 8 0.3962208392103932 2213 9 0.529066296040817 2213 10-11 0.5240083652340033 2213 12-13 0.5311124158627933 2213 14-15 0.6157880243956413 2213 16-17 0.6677802375511703 2213 18-19 0.5820864019539655 2213 20-21 0.43506608053134244 2213 22-23 0.6766807444065535 2213 24-25 0.7180515426786727 2213 26-27 0.7011811641186512 2213 28-29 0.7309943179912253 2213 30-31 0.7018434829437581 2213 32-33 0.7600983450333416 2213 34-35 0.63058642197322 2213 36-37 0.6878539937286874 2213 38-39 0.6925412425085966 2213 40-41 0.594209220975948 2213 42-43 0.7262988149110896 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3 0.3606559284950066 2311 4 0.29452761638744107 2311 5 0.28186046842557744 2311 6 0.3888930959029011 2311 7 0.37228113549746666 2311 8 0.19985744703731 2311 9 0.5155155517174848 2311 10-11 0.38627146224175135 2311 12-13 0.5050581994550214 2311 14-15 0.44360499136581666 2311 16-17 0.47002923255441686 2311 18-19 0.6217948384323719 2311 20-21 0.6823337408500691 2311 22-23 0.667073677531917 2311 24-25 0.5965206510473848 2311 26-27 0.6641295208688405 2311 28-29 0.76656647172139 2311 30-31 0.695991456314772 2311 32-33 0.6499897718082224 2311 34-35 0.7426912529780978 2311 36-37 0.8097612440514794 2311 38-39 0.8008620356395255 2311 40-41 0.8342280846962566 2311 42-43 0.8012616521611307 2311 44-45 0.7945340216118311 2311 46-47 0.8587744093557248 2311 48-49 -6.102535970100458 2311 50-51 -1.0624843363017078 2311 52-53 0.37743992887901356 2311 54-55 0.8806812277145326 2311 56-57 1.0130461632282461 2311 58-59 0.9996444395463229 2311 60-61 0.8555310539138077 2311 62-63 0.7767582426604065 2311 64-65 0.8776320121991645 2311 66-67 0.8770953929160186 2311 68-69 0.8703424594391649 2311 70-71 0.9082642242808845 2311 72-73 0.35013016832039057 2311 74-75 0.6909145128157661 2311 76-77 0.8047673785042093 2311 78-79 0.748790220192106 2311 80-81 0.848681878134844 2311 82-83 0.8500704255143141 2311 84-85 0.9246817979892263 2311 86-87 0.945678499795207 2311 88-89 1.0403236924436108 2311 90-91 1.0863430247749655 2311 92-93 1.0491451899234292 2311 94-95 1.0901814196113513 2311 96-97 1.1410130028037884 2311 98-99 1.0106134527212909 2311 100 1.3148565109132946 2312 1 0.09379678922306667 2312 2 0.2531647606492413 2312 3 0.266131439573968 2312 4 0.042886537160157445 2312 5 0.2759576069192704 2312 6 0.23353815391002541 2312 7 0.33930883959413904 2312 8 0.4179468289197956 2312 9 0.32922794086091045 2312 10-11 0.3098783869262647 2312 12-13 0.35747661437343936 2312 14-15 0.3489708180678477 2312 16-17 0.5265845067821004 2312 18-19 0.36443258861321937 2312 20-21 0.40841584839770206 2312 22-23 0.6429272061871671 2312 24-25 0.7561013720505159 2312 26-27 0.6996253947267874 2312 28-29 0.814553805269064 2312 30-31 0.8338915112406937 2312 32-33 0.8179810238512033 2312 34-35 0.36726340017955295 2312 36-37 0.6883665946395823 2312 38-39 0.8358304197458324 2312 40-41 0.5793709925546793 2312 42-43 0.6087538486619479 2312 44-45 0.7418595648425494 2312 46-47 0.8665452544110579 2312 48-49 -4.7023772208037755 2312 50-51 -0.8412702056048218 2312 52-53 0.5822332198791997 2312 54-55 0.8547898537628171 2312 56-57 0.8896537510427294 2312 58-59 0.9001967129434192 2312 60-61 0.9378328517328427 2312 62-63 0.7256696042872832 2312 64-65 0.959782261375274 2312 66-67 1.058789656506839 2312 68-69 0.9559439987044982 2312 70-71 0.981836485353142 2312 72-73 1.1260240541280666 2312 74-75 0.8041113016591055 2312 76-77 0.9810988296201408 2312 78-79 0.6528013192463114 2312 80-81 0.8856580724386198 2312 82-83 0.8474413496093725 2312 84-85 0.8879961416915023 2312 86-87 0.9878801917813824 2312 88-89 1.0634587231217445 2312 90-91 0.729761627245292 2312 92-93 0.9266444866037595 2312 94-95 1.1364498063969783 2312 96-97 1.2647754951007606 2312 98-99 1.2219241726526988 2312 100 0.9202020753105806 2313 1 0.07783968335079905 2313 2 0.2876620865456445 2313 3 0.38182440712076726 2313 4 0.10152945871918462 2313 5 0.20430577198040112 2313 6 0.283114739233028 2313 7 0.23057488376749546 2313 8 0.35618471746265357 2313 9 0.576977744987083 2313 10-11 0.30654141625623765 2313 12-13 0.05445318750474826 2313 14-15 0.13337373681203246 2313 16-17 0.13181906681452205 2313 18-19 0.4475610276108526 2313 20-21 0.45223029178433194 2313 22-23 0.5866464804410043 2313 24-25 0.37008699885574714 2313 26-27 0.49045152958479576 2313 28-29 0.46808712040451184 2313 30-31 0.5939677518795392 2313 32-33 0.5413053165091171 2313 34-35 0.4332265458684432 2313 36-37 -0.21426853589216677 2313 38-39 0.2274837293412304 2313 40-41 0.44415927625170326 2313 42-43 0.48372902826525177 2313 44-45 0.5281316891726746 2313 46-47 0.462850242515195 2313 48-49 -4.985259147879546 2313 50-51 -1.1975241387692677 2313 52-53 0.23460378627281386 2313 54-55 0.3960061114700295 2313 56-57 0.45258908847461754 2313 58-59 0.6126065303011572 2313 60-61 0.8579831143023853 2313 62-63 0.9818478660571941 2313 64-65 1.1170269821945524 2313 66-67 1.141532454893948 2313 68-69 1.081623562116519 2313 70-71 1.0305811671769476 2313 72-73 0.7499322928413434 2313 74-75 0.8870938437876674 2313 76-77 0.9180807388566024 2313 78-79 0.8509685430552452 2313 80-81 -0.04824179345279944 2313 82-83 0.39398200261776495 2313 84-85 0.8044898499730628 2313 86-87 0.9610420593150941 2313 88-89 1.0767404367533082 2313 90-91 1.0068353573822542 2313 92-93 1.1170094775406731 2313 94-95 1.0146770182473013 2313 96-97 1.159570966067509 2313 98-99 1.3611335416072805 2313 100 1.4190166352354225 2314 1 0.03600743141441143 2314 2 0.19478031792035466 2314 3 0.21428488927946177 2314 4 0.08446831934120524 2314 5 0.22022334718346315 2314 6 0.29550918953648875 2314 7 0.03097890164023198 2314 8 0.28355949833135696 2314 9 0.3998589810622022 2314 10-11 0.3119906703367903 2314 12-13 0.21628601564295025 2314 14-15 0.19356385503816398 2314 16-17 0.4362553515525036 2314 18-19 0.5293555492411173 2314 20-21 0.5821766242597661 2314 22-23 0.3281500681703591 2314 24-25 0.5085299618600985 2314 26-27 0.4936661464280476 2314 28-29 0.6144475656339097 2314 30-31 0.49958736744815724 2314 32-33 0.4613513668202671 2314 34-35 0.3629794142185503 2314 36-37 0.3987577066084782 2314 38-39 0.47736859810690646 2314 40-41 0.28428973576392735 2314 42-43 0.32889148662157197 2314 44-45 0.5323785039115734 2314 46-47 0.5417638096234967 2314 48-49 -6.475108392531347 2314 50-51 -1.6928005388523815 2314 52-53 -0.866998709554089 2314 54-55 -0.3951346437210219 2314 56-57 -0.022630195603348113 2314 58-59 0.4459628535049802 2314 60-61 0.6620891742867343 2314 62-63 0.4102960410688894 2314 64-65 0.579835282905556 2314 66-67 0.764684571300684 2314 68-69 0.8241554736573207 2314 70-71 0.6147548675799399 2314 72-73 0.6904120637923867 2314 74-75 0.7188680673993701 2314 76-77 0.737155114288754 2314 78-79 0.4975252570286628 2314 80-81 0.5928007891710507 2314 82-83 0.5968855796087986 2314 84-85 0.6334070183427265 2314 86-87 0.6802226283056534 2314 88-89 0.6010929299611121 2314 90-91 0.7933012388138856 2314 92-93 0.8831496761731543 2314 94-95 0.9824434373799953 2314 96-97 0.9795225214632026 2314 98-99 1.0789286617017098 2314 100 -0.23689855658739845 2315 1 0.1312395945329463 2315 2 0.23286129387747678 2315 3 0.3425394509791957 2315 4 0.16436745396921282 2315 5 0.25782443771546326 2315 6 0.25980638728337624 2315 7 0.2716798457971805 2315 8 0.24150434296674206 2315 9 0.41566879113657507 2315 10-11 0.23206360113733382 2315 12-13 0.4051803242712495 2315 14-15 0.2287080973810447 2315 16-17 0.37258996436987957 2315 18-19 0.4598044372873531 2315 20-21 0.374217496693781 2315 22-23 0.4611222318696804 2315 24-25 0.4757765453199667 2315 26-27 0.5508218052251905 2315 28-29 0.636787463188206 2315 30-31 0.5798562011361383 2315 32-33 0.6422694609733384 2315 34-35 0.709161379070018 2315 36-37 0.5143397578624729 2315 38-39 0.6655135131942274 2315 40-41 0.7171399448050053 2315 42-43 0.7641341522972596 2315 44-45 0.6633665239259798 2315 46-47 0.6523484659998573 2315 48-49 -4.746744375710421 2315 50-51 -0.8973241349902281 2315 52-53 0.2891094606820275 2315 54-55 0.24278130783464746 2315 56-57 0.5199458264126662 2315 58-59 0.6877438818170631 2315 60-61 0.582235939684324 2315 62-63 0.28293449132830517 2315 64-65 0.5234350412057509 2315 66-67 -0.12092882980230257 2315 68-69 0.48027035134703766 2315 70-71 0.5473620563709289 2315 72-73 0.608937545592731 2315 74-75 0.699467139277175 2315 76-77 0.6913857656787705 2315 78-79 0.6409236707237795 2315 80-81 0.49155235582178136 2315 82-83 0.5074014148808956 2315 84-85 0.5503342323179687 2315 86-87 0.5661153488193769 2315 88-89 0.5992314143101751 2315 90-91 0.7197779991443163 2315 92-93 0.7407528036667479 2315 94-95 0.3901145232151997 2315 96-97 0.5282653500116403 2315 98-99 0.5992900708751066 2315 100 0.6407508612792796 2316 1 0.2347203656947947 2316 2 0.3036971027940325 2316 3 0.17659363946633277 2316 4 9.33719019961643E-4 2316 5 0.14017873272953096 2316 6 0.1033337112326933 2316 7 0.2107505209197953 2316 8 0.25504129705959144 2316 9 0.3931366093428821 2316 10-11 0.3370971652903947 2316 12-13 0.32870935492140063 2316 14-15 0.4319213610528152 2316 16-17 0.39296733126784034 2316 18-19 0.03629290452445133 2316 20-21 0.3716504042504525 2316 22-23 0.587316679427488 2316 24-25 0.6518450947908221 2316 26-27 0.6263189094299477 2316 28-29 0.6049174786909148 2316 30-31 0.43568086158648356 2316 32-33 0.46606641948322647 2316 34-35 0.4452032802569832 2316 36-37 0.28255310114175103 2316 38-39 0.4164478241812901 2316 40-41 -0.04959120131344008 2316 42-43 -0.12983498279583472 2316 44-45 0.1752066033253996 2316 46-47 0.28459071936201497 2316 48-49 -5.308443029618445 2316 50-51 -1.2212281563988157 2316 52-53 0.06813247849587611 2316 54-55 0.3022185568641049 2316 56-57 0.23677155307812114 2316 58-59 0.1812682447875602 2316 60-61 0.39223536745610943 2316 62-63 0.28218778344019313 2316 64-65 0.2712051771885058 2316 66-67 0.2612031684790139 2316 68-69 -0.0647931977741294 2316 70-71 -0.5805249570912494 2316 72-73 -0.40236267406618254 2316 74-75 -0.049250877750839805 2316 76-77 -0.01561318954497537 2316 78-79 0.06504242746578726 2316 80-81 0.05727917575837793 2316 82-83 -0.10210863954699434 2316 84-85 0.11100277036528539 2316 86-87 0.08166332510989704 2316 88-89 0.0026588142886012633 2316 90-91 0.13479196934189375 2316 92-93 0.24138926961057905 2316 94-95 0.47856949602805443 2316 96-97 0.6035261276461235 2316 98-99 0.4884787884265762 2316 100 -0.12685076532011763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 2.0 11 4.0 12 25.0 13 207.0 14 837.0 15 2303.0 16 4628.0 17 8196.0 18 12722.0 19 18255.0 20 24419.0 21 31024.0 22 35254.0 23 35655.0 24 37015.0 25 41396.0 26 48892.0 27 58954.0 28 70387.0 29 85093.0 30 102513.0 31 124532.0 32 153523.0 33 195232.0 34 261823.0 35 368599.0 36 556824.0 37 762050.0 38 507595.0 39 46941.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.38900045048191 20.567017322824128 14.736935537680349 24.30704668901361 2 16.408638415322464 21.53111165881186 37.84747857059006 24.21277135527561 3 19.185279598720633 24.68565207062668 30.020456746804225 26.10861158384846 4 14.423015390299943 17.387583254917388 35.80491485725702 32.38448649752566 5 14.910358870507858 36.26341835216099 33.40633510343849 15.419887673892665 6 31.803055604003777 35.593728120063325 17.262385104688498 15.340831171244398 7 27.432195610448133 32.06392389218059 21.88277838048346 18.621102116887815 8 25.533256091166155 33.82606046865503 20.69289840336824 19.947785036810576 9 25.753144861422456 16.86593812820029 21.40620053335648 35.97471647702077 10-11 25.32827741581843 26.028123181867684 26.98923060639159 21.654368795922295 12-13 25.503818048852 23.908043035423248 27.417446220324965 23.170692695399786 14-15 23.179462571976966 25.743859356310328 25.384405685832707 25.692272385879996 16-17 22.489212125258266 28.168046749659414 26.488455496235286 22.854285628847038 18-19 22.186758411427743 27.35521784883645 28.68339072480716 21.774633014928646 20-21 22.905862293469482 26.671170270871347 28.44453049230736 21.97843694335181 22-23 23.2102787938401 26.22270210004655 28.106080383677252 22.460938722436097 24-25 22.87375606814262 26.328078808278892 28.250301155678397 22.547863967900096 26-27 22.647449450283965 26.651105739614838 28.2077269711006 22.493717839000595 28-29 22.469471088114148 26.76460434880695 28.25838534957165 22.507539213507243 30-31 22.804546151887138 26.617768536704407 28.14781711632055 22.429868195087906 32-33 22.7437205249699 26.76053144633792 27.82519345857416 22.670554570118025 34-35 22.870644665647905 26.453500787025863 28.154631280295973 22.52122326703026 36-37 22.841849171435353 25.99766784906515 28.187168606833353 22.973314372666145 38-39 22.578380276360658 26.02747691800885 28.415945493558407 22.978197312072083 40-41 22.490145350858892 25.67175742402114 28.778065234021838 23.06003199109813 42-43 22.259551455738105 25.819224756171515 28.519920773129627 23.401303014960746 44-45 21.881398899046836 25.98310666309651 28.659834683154305 23.47565975470235 46-47 21.871057798438873 25.93514984973644 28.47411370232583 23.719678649498853 48-49 22.030743093520794 25.93426228531651 28.39005111550143 23.644943505661264 50-51 21.540207464510143 25.997304344112482 28.32836922556441 24.134118965812966 52-53 21.386211116893495 26.174399920094604 28.224716112580357 24.214672850431544 54-55 21.269261691940898 26.092578528168875 28.072606737384444 24.56555304250578 56-57 21.33260650578351 25.878515012865073 27.755794289071687 25.033084192279738 58-59 21.034019612179705 25.812057687594432 27.80932970901098 25.34459299121489 60-61 21.23926215333198 25.774267613448508 27.570354550799532 25.41611568241998 62-63 21.2238976956028 25.562175659863694 27.44684674759904 25.767079896934465 64-65 20.90535095064159 25.533323474941298 27.378127251438105 26.183198322979006 66-67 21.407323211595155 25.46804807570574 26.907856752316068 26.216771960383035 68-69 21.247779578353615 25.53322118070782 26.646256224415065 26.572743016523496 70-71 21.231532678958853 25.312884536594492 26.480700219183685 26.97488256526297 72-73 21.504158711592364 25.32724082687885 26.2952604543727 26.873340007156088 74-75 21.5538819577061 25.36472574229372 26.30690695962218 26.774485340378007 76-77 21.46544719716437 25.27553590587815 26.190444063821978 27.0685728331355 78-79 21.481694454018935 25.073018824515852 26.11426907036931 27.3310176510959 80-81 21.317632037209094 25.125833838690113 26.16359094474828 27.392943179352514 82-83 21.454169619737247 24.616708247291463 26.374473229009666 27.554648903961628 84-85 21.51037092487835 24.466582204414696 26.189484157484866 27.833562713222086 86-87 21.30516020584231 24.528043865095412 26.415033705836255 27.751762223226024 88-89 21.235862607051292 24.05991867190452 26.382700054927348 28.321518666116834 90-91 21.275467157270196 23.76890626249839 26.25406817209611 28.701558408135302 92-93 21.443198987783234 23.50936308852974 26.424869529365385 28.62256839432164 94-95 21.159349210590484 23.23416503914604 26.788194388903268 28.81829136136021 96-97 21.34727892869075 23.05030434416135 26.693560299534408 28.908856427613493 98-99 21.21398862103023 23.111049285684476 26.548777943160793 29.1261841501245 100 21.125640434855683 23.016558086314422 26.66863680734807 29.189164671481826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4230.0 1 3008.0 2 1811.0 3 1870.5 4 1742.5 5 1789.0 6 2497.0 7 3881.0 8 5075.0 9 5236.5 10 4448.5 11 3658.0 12 3511.0 13 3548.0 14 3691.0 15 3916.0 16 4179.0 17 4504.5 18 4844.0 19 5273.0 20 5765.0 21 6328.5 22 6974.0 23 7783.0 24 8747.0 25 9861.5 26 11231.5 27 12839.5 28 14472.5 29 16467.5 30 19252.5 31 22602.5 32 26908.0 33 32222.0 34 38994.0 35 46651.5 36 55656.0 37 65569.5 38 73696.0 39 80036.0 40 83761.0 41 87401.0 42 94006.0 43 104062.0 44 116455.5 45 130071.5 46 143483.0 47 155025.0 48 163713.0 49 169075.0 50 171676.0 51 171611.0 52 169736.5 53 165416.5 54 159270.5 55 148940.5 56 135046.0 57 117228.5 58 99078.5 59 84584.5 60 69456.5 61 54860.5 62 42709.5 63 32682.5 64 24064.0 65 17273.0 66 12457.0 67 9464.0 68 7327.5 69 5506.5 70 4252.5 71 3303.0 72 2549.0 73 2020.0 74 1559.5 75 1131.5 76 803.0 77 486.0 78 272.0 79 164.5 80 101.0 81 50.5 82 27.0 83 27.0 84 21.0 85 11.5 86 8.5 87 4.5 88 3.5 89 2.5 90 1.5 91 1.0 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027093916024994277 2 0.0021419214927356875 3 2.7817162243320614E-5 4 0.02283789020176622 5 3.6162310916316797E-4 6 0.0 7 8.345148672996183E-5 8 0.004116940012011451 9 0.008595503133186069 10-11 0.0010987779086111642 12-13 1.1126864897328245E-4 14-15 8.345148672996183E-5 16-17 2.9208020355486644E-4 18-19 5.563432448664123E-5 20-21 1.2517723009494274E-4 22-23 1.8081155458158398E-4 24-25 4.172574336498092E-5 26-27 4.172574336498092E-5 28-29 8.901491917862596E-4 30-31 0.0022949158850739506 32-33 0.009318749351512405 34-35 0.018790493095363073 36-37 0.022142461145683207 38-39 0.021113226142680346 40-41 0.0042560258232280535 42-43 0.00453419744566126 44-45 0.008080885631684638 46-47 0.010723516044800097 48-49 1.2354853524559635 50-51 0.01673202308935735 52-53 0.018150698363766697 54-55 0.009263115027025764 56-57 0.007955708401589694 58-59 0.008345148672996183 60-61 0.005549523867542462 62-63 0.008261697186266221 64-65 0.018707041608633113 66-67 0.027483356296400765 68-69 0.02756680778313073 70-71 0.024632097166460403 72-73 0.021168860467166987 74-75 0.008859766174497616 76-77 0.013866855378295325 78-79 0.007844439752616412 80-81 0.002308824466195611 82-83 0.013366146457915553 84-85 0.022045101077831586 86-87 0.022045101077831586 88-89 0.029625277789136456 90-91 0.01920775052901288 92-93 0.011571939493221375 94-95 0.006189318599138836 96-97 0.003449328118171756 98-99 0.015382890720556298 100 0.034799269966394084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3594903.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.12755608361442 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21294409278727 46.587110143292236 2 13.98103513356013 17.09253018467361 3 4.456817051513232 8.173030028123524 4 1.8286323726252194 4.471193116558441 5 0.999926123792959 3.056152010581264 6 0.5969328956051175 2.18934294325537 7 0.4056347917466791 1.7356824437372267 8 0.2854397730178769 1.395858858691555 9 0.2059064410665299 1.132790177184458 >10 0.9839189465119493 10.016797331369103 >50 0.03158291729489575 1.3029368657686577 >100 0.010350934670437836 1.0165650663051693 >500 3.617459216664092E-4 0.15046074829915887 >1k 3.100679315970224E-4 0.4653132092653904 >5k 1.550339657985112E-4 0.7713206130775992 >10k+ 5.167798859950374E-5 0.4429162598173821 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14021 0.3900244318135983 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9923 0.27602970094047047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7884 0.2193105071263397 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6610 0.18387144242834924 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4513 0.12553885320410593 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7817162243320614E-5 2 0.0 0.0 0.0 0.0 2.7817162243320614E-5 3 0.0 0.0 0.0 0.0 2.7817162243320614E-5 4 0.0 0.0 0.0 0.0 5.563432448664123E-5 5 0.0 0.0 0.0 0.0 5.563432448664123E-5 6 0.0 0.0 0.0 0.0 1.1126864897328245E-4 7 0.0 0.0 0.0 0.0 1.3908581121660306E-4 8 0.0 0.0 0.0 0.0 1.9472013570324427E-4 9 0.0 0.0 0.0 2.7817162243320614E-5 2.503544601898855E-4 10-11 0.0 0.0 0.0 2.7817162243320614E-5 2.503544601898855E-4 12-13 0.0 0.0 0.0 2.7817162243320614E-5 2.781716224332061E-4 14-15 0.0 0.0 0.0 2.7817162243320614E-5 4.0334885252814887E-4 16-17 0.0 0.0 0.0 5.563432448664123E-5 4.172574336498092E-4 18-19 0.0 0.0 0.0 5.563432448664123E-5 5.00708920379771E-4 20-21 0.0 0.0 0.0 3.4771452804150765E-4 5.00708920379771E-4 22-23 0.0 0.0 0.0 0.002225372979465649 5.563432448664122E-4 24-25 0.0 0.0 0.0 0.006384038734842081 6.119775693530535E-4 26-27 0.0 0.0 0.0 0.017093646198520517 6.397947315963741E-4 28-29 0.0 0.0 0.0 0.04705272993457682 6.676118938396947E-4 30-31 0.0 0.0 0.0 0.1656372925778526 6.676118938396947E-4 32-33 0.0 0.0 0.0 0.48300329661189745 7.232462183263359E-4 34-35 0.0 0.0 0.0 0.9856455097675791 8.345148672996184E-4 36-37 0.0 0.0 0.0 1.701700991654017 8.345148672996184E-4 38-39 0.0 0.0 0.0 2.5556044210372297 8.62332029542939E-4 40-41 0.0 0.0 0.0 3.4082004437950064 8.62332029542939E-4 42-43 0.0 0.0 0.0 4.273953984293874 8.62332029542939E-4 44-45 0.0 0.0 0.0 5.337668359897332 8.762406106645993E-4 46-47 0.0 0.0 0.0 6.4729145682094895 9.179663540295802E-4 48-49 0.0 0.0 0.0 7.513137350298464 0.0010292350030028626 50-51 0.0 0.0 0.0 8.534333193413007 0.0010292350030028626 52-53 0.0 0.0 0.0 9.509547267339341 0.0011126864897328245 54-55 0.0 0.0 0.0 10.584861399598264 0.0011683208142194658 56-57 0.0 0.0 0.0 11.802474225312894 0.001182229395341126 58-59 0.0 0.0 0.0 13.036457451007719 0.0012100465575844467 60-61 0.0 0.0 0.0 14.237741602485519 0.001223955138706107 62-63 0.0 0.0 0.0 15.440611332211189 0.0012517723009494277 64-65 0.0 0.0 0.0 16.717905879518863 0.0012517723009494277 66-67 0.0 0.0 0.0 18.10370126815661 0.0012795894631927483 68-69 0.0 0.0 0.0 19.550569236499566 0.0013074066254360687 70-71 0.0 0.0 0.0 21.055157816497413 0.0013491323688010497 72-73 0.0 0.0 0.0 22.532875017768212 0.0015855782478692748 74-75 0.0 0.0 0.0 24.014472713171955 0.0016133954101125955 76-77 0.0 0.0 0.0 25.55817222328391 0.0016133954101125955 78-79 0.0 0.0 0.0 27.144584986020483 0.0016133954101125955 80-81 0.0 0.0 0.0 28.758022121876444 0.0016690297345992367 82-83 0.0 0.0 0.0 30.3328629451198 0.0017524812213291986 84-85 0.0 0.0 0.0 31.88539440424401 0.001766389802450859 86-87 0.0 0.0 0.0 33.45933116971445 0.00180811554581584 88 0.0 0.0 0.0 34.63587195537682 0.00180811554581584 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5545 0.0 50.523182 1 GTATCAA 10635 0.0 43.948067 1 GTGGTAT 2270 0.0 39.89784 1 ATCAACG 13655 0.0 33.53587 3 TATCAAC 13830 0.0 33.514896 2 CAACGCA 14105 0.0 32.45918 5 TGGTATC 2910 0.0 31.276047 2 TCAACGC 14775 0.0 31.088991 4 AACGCAG 15430 0.0 30.006271 6 ACGCAGA 17300 0.0 26.35609 7 CGCAGAG 18040 0.0 25.144949 8 GCAGAGT 19105 0.0 23.250889 9 GTACATG 11180 0.0 21.490479 1 TACATGG 11655 0.0 20.488426 2 GAGTACT 11900 0.0 20.277512 12-13 ACATGGG 11930 0.0 19.42844 3 CAGAGTA 18485 0.0 19.181856 10-11 CATGGGG 7865 0.0 18.910889 4 AGTACTT 12655 0.0 18.826857 12-13 AGAGTAC 18025 0.0 18.656588 10-11 >>END_MODULE