FastQCFastQC Report
Mon 6 Jun 2016
ERR1211364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551758
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113110.728915204561536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97590.6288996093463027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76780.4947936469475266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64570.41610869736131534No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62270.4012867985858619No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.3066844185755769No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33420.21536863351115315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.14931451940315435No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.139390291527416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49200.035.75151
GTATCAA80700.033.8019831
TACCTGG7800.031.9572222
ATCAACG97300.027.5278023
TCAACGC98700.027.1849464
CAACGCA98700.027.1373375
TATCAAC101650.026.4190062
AACGCAG103150.026.103276
CCGTATC6250.024.82616294
ACGCAGA112250.023.8630547
CGCAGAG113200.023.5812828
GCAGAGT116650.022.3219729
CGTATCA5300.022.17888894
GTGGTAT32950.021.124871
ACCTGGG11400.021.022043
GAGTACT69850.019.74886912-13
GCGGTAT3102.9649527E-1019.7228371
GTACATG68200.019.5849171
TGGTATC35200.019.5073362
CGCCGTT1708.602795E-519.36086594
Produced by FastQC (version 0.11.5)