##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211364_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1551758 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77027216872734 31.0 31.0 34.0 30.0 34.0 2 31.787452038268853 33.0 31.0 34.0 30.0 34.0 3 31.837822005750898 33.0 31.0 34.0 30.0 34.0 4 35.49095219744316 37.0 35.0 37.0 33.0 37.0 5 35.53449764718468 37.0 35.0 37.0 33.0 37.0 6 35.60052791736856 37.0 35.0 37.0 33.0 37.0 7 35.55632450420749 37.0 35.0 37.0 33.0 37.0 8 35.56753630398554 37.0 35.0 37.0 33.0 37.0 9 37.18661994976021 39.0 37.0 39.0 33.0 39.0 10-11 37.14369025324825 39.0 37.0 39.0 33.0 39.0 12-13 37.09831913223583 39.0 37.0 39.0 33.0 39.0 14-15 38.277922846217 40.0 38.0 41.0 33.0 41.0 16-17 38.27583456956562 40.0 38.0 41.0 33.0 41.0 18-19 38.27201180854232 40.0 38.0 41.0 33.5 41.0 20-21 38.23717841312885 40.0 38.0 41.0 34.0 41.0 22-23 38.09703252697908 40.0 38.0 41.0 33.0 41.0 24-25 37.98455944805826 40.0 37.0 41.0 33.0 41.0 26-27 37.84373562114711 40.0 37.0 41.0 33.0 41.0 28-29 37.697109343080555 40.0 37.0 41.0 33.0 41.0 30-31 37.48666512432995 40.0 36.5 41.0 32.0 41.0 32-33 37.30487614692497 39.5 36.0 41.0 32.0 41.0 34-35 37.131258546757934 39.0 35.5 41.0 31.5 41.0 36-37 37.013352597505545 39.0 35.0 41.0 31.0 41.0 38-39 36.85312497180617 39.0 35.0 41.0 31.0 41.0 40-41 36.69234152490272 39.0 35.0 41.0 30.0 41.0 42-43 36.5059838583078 39.0 35.0 41.0 30.0 41.0 44-45 36.357363068210375 39.0 35.0 41.0 30.0 41.0 46-47 36.1638960456463 39.0 35.0 41.0 28.5 41.0 48-49 35.91357930811377 39.0 35.0 41.0 27.5 41.0 50-51 35.20844648456783 38.0 34.0 39.5 26.5 40.5 52-53 35.41366598400008 38.0 34.5 40.0 27.0 40.5 54-55 35.776721950201 39.0 35.0 40.5 27.5 41.0 56-57 35.69144673331795 39.0 35.0 41.0 27.0 41.0 58-59 35.48479112078043 38.5 35.0 40.5 26.5 41.0 60-61 35.225327982842686 38.0 34.5 40.0 26.0 41.0 62-63 34.936293867987146 38.0 34.0 40.0 26.0 41.0 64-65 34.63889923557669 37.0 34.0 40.0 25.5 41.0 66-67 34.3781875137747 37.0 34.0 40.0 25.0 41.0 68-69 33.96824762624069 36.0 33.5 39.0 23.5 41.0 70-71 33.53488623870474 35.5 33.0 39.0 23.5 40.5 72-73 33.06105784535991 35.0 33.0 38.5 21.5 40.0 74-75 32.56574188758814 35.0 32.0 37.0 20.0 39.5 76-77 32.07594515381909 35.0 31.5 37.0 19.5 39.0 78-79 31.577130583505934 35.0 31.0 36.0 18.0 39.0 80-81 31.083032921370474 35.0 30.5 36.0 16.0 37.0 82-83 30.621975849327022 34.5 30.0 35.0 12.0 37.0 84-85 30.18449332950112 34.0 29.0 35.0 10.5 36.5 86-87 29.78105864445358 34.0 29.0 35.0 10.0 36.0 88-89 29.34130096316565 34.0 28.5 35.0 10.0 36.0 90-91 28.92787180733078 34.0 27.0 35.0 8.0 35.0 92-93 28.523141817216345 34.0 25.5 35.0 7.5 35.0 94-95 28.11920254318006 34.0 25.0 35.0 7.0 35.0 96-97 27.73746196249673 33.0 24.0 35.0 7.0 35.0 98-99 27.312449814984035 33.0 23.0 35.0 7.0 35.0 100 25.44873169656609 31.0 18.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3112661007778428 1101 2 -0.37308138487283315 1101 3 -0.33442276072879196 1101 4 -0.3387920005793532 1101 5 -0.35520165623506017 1101 6 -0.3991639646347167 1101 7 -0.4251355820443621 1101 8 -0.3479274010400246 1101 9 -0.5379844928228366 1101 10-11 -0.3837177441972912 1101 12-13 -0.48374162081302785 1101 14-15 -0.5510912601830924 1101 16-17 -0.3772255035106724 1101 18-19 -0.5409074767948852 1101 20-21 -0.460401821540934 1101 22-23 -0.5123597129954334 1101 24-25 -0.641433956045951 1101 26-27 -0.6377238586206246 1101 28-29 -0.6924828740716222 1101 30-31 -0.5773959916805111 1101 32-33 -0.41427899762350506 1101 34-35 -0.38771879077253857 1101 36-37 -0.6615451492030928 1101 38-39 -0.7785757149157888 1101 40-41 -0.8281606176157226 1101 42-43 -0.7914734888519277 1101 44-45 -1.1266289786350612 1101 46-47 -0.8880417016788726 1101 48-49 -0.8770697651387067 1101 50-51 -0.8816953413809188 1101 52-53 -0.8197904480412106 1101 54-55 -0.7672685892092446 1101 56-57 -0.8423835104106558 1101 58-59 -1.0349393583379722 1101 60-61 -0.9038842662801514 1101 62-63 -0.8448687139773341 1101 64-65 -0.9806342242879182 1101 66-67 -1.0210873344019546 1101 68-69 -0.9045231819346711 1101 70-71 -1.1278748501883769 1101 72-73 -1.1818421275680997 1101 74-75 -1.1315048233245406 1101 76-77 -1.1064876999632496 1101 78-79 -1.2467702438919552 1101 80-81 -1.2832863884891665 1101 82-83 -1.4055067889475232 1101 84-85 -1.4911607194209147 1101 86-87 -1.5145188818881152 1101 88-89 -1.5828515398233556 1101 90-91 -1.494715725246511 1101 92-93 -1.5231501206748383 1101 94-95 -1.4729849903968564 1101 96-97 -1.4609979114459044 1101 98-99 -1.634549031369513 1101 100 -1.732415193290997 1102 1 -0.3705534054728439 1102 2 -0.2776346626698043 1102 3 -0.43246715142836933 1102 4 -0.23553147874230262 1102 5 -0.2403188072453304 1102 6 -0.3747836301369105 1102 7 -0.3779680644173311 1102 8 -0.346916485713777 1102 9 -0.4569049248291819 1102 10-11 -0.3455644881541886 1102 12-13 -0.29028169912697166 1102 14-15 -0.5048218896378458 1102 16-17 -0.5377355038945595 1102 18-19 -0.5734463138583692 1102 20-21 -0.5502741135035123 1102 22-23 -0.5503858048479771 1102 24-25 -0.5888655593064769 1102 26-27 -0.5016885661589825 1102 28-29 -0.6537433831122783 1102 30-31 -0.6405797991203386 1102 32-33 -0.669969395207545 1102 34-35 -0.5887197888914741 1102 36-37 -0.611156938523223 1102 38-39 -0.7775384070216873 1102 40-41 -0.7271026755322083 1102 42-43 -0.634263359224434 1102 44-45 -0.45190378125064257 1102 46-47 -0.6029467844075711 1102 48-49 -0.7934988986946934 1102 50-51 -0.5197548638194007 1102 52-53 -0.3381020914824191 1102 54-55 -0.46592624721274944 1102 56-57 -0.4134043109532257 1102 58-59 -0.4478966695591353 1102 60-61 -0.7469252659346424 1102 62-63 -0.46275311772923544 1102 64-65 -0.6354866178538856 1102 66-67 -0.4597542058109667 1102 68-69 -0.37064472210895616 1102 70-71 -0.5207122271830187 1102 72-73 -0.7058681618368539 1102 74-75 -0.5922062897915836 1102 76-77 -0.8842006959835693 1102 78-79 -0.8505714134826015 1102 80-81 -0.9157994728046219 1102 82-83 -0.9568691253135988 1102 84-85 -1.04392746030668 1102 86-87 -1.261802846723615 1102 88-89 -1.4402524957141765 1102 90-91 -1.3433767423041054 1102 92-93 -1.5327978038872487 1102 94-95 -1.4822255882445 1102 96-97 -1.5933838315707938 1102 98-99 -1.7572600759393637 1102 100 -1.8052618796641156 1103 1 -0.5013245803100084 1103 2 -0.29705799305996905 1103 3 -0.36658650341885135 1103 4 -0.3453709479477709 1103 5 -0.22142972641049852 1103 6 -0.5672926196054746 1103 7 -0.34838119607945117 1103 8 -0.4524595647827141 1103 9 -0.5591833232321903 1103 10-11 -0.589858095074483 1103 12-13 -0.6123965915732654 1103 14-15 -0.7451702075515172 1103 16-17 -0.5731319362592089 1103 18-19 -0.6483636171457619 1103 20-21 -0.7593784297280664 1103 22-23 -0.8000059118258491 1103 24-25 -0.7916532542915675 1103 26-27 -0.8208378937083438 1103 28-29 -0.6559331664692891 1103 30-31 -0.7901152899261277 1103 32-33 -0.8590889391439731 1103 34-35 -0.694005340480139 1103 36-37 -1.001091932828821 1103 38-39 -0.7263096330444441 1103 40-41 -0.7495787462706929 1103 42-43 -0.821078752009825 1103 44-45 -0.9515558792198604 1103 46-47 -0.8946206490472903 1103 48-49 -0.8799937417468939 1103 50-51 -0.9873239963516767 1103 52-53 -0.9301705650002745 1103 54-55 -0.9781604020747423 1103 56-57 -0.8255706449135829 1103 58-59 -0.8737551478116572 1103 60-61 -0.8220129212509093 1103 62-63 -0.7684798250884484 1103 64-65 -0.8008096628844044 1103 66-67 -0.8226224221212561 1103 68-69 -0.928280491876194 1103 70-71 -1.1081380080831096 1103 72-73 -1.1869590866324273 1103 74-75 -1.1209054081198637 1103 76-77 -1.0856543666299174 1103 78-79 -1.2445772614358148 1103 80-81 -1.3169121194833195 1103 82-83 -1.4669102977194513 1103 84-85 -1.4385291404735447 1103 86-87 -1.4651767766249577 1103 88-89 -1.7864334111683853 1103 90-91 -1.5053151404511844 1103 92-93 -1.4928138633648977 1103 94-95 -1.8965961015079635 1103 96-97 -2.1433809523815768 1103 98-99 -2.142955464118053 1103 100 -2.0365087605424605 1104 1 -0.42276864746714793 1104 2 -0.5087680477666261 1104 3 -0.6417845792535992 1104 4 -0.34945036692532483 1104 5 -0.2924493453503203 1104 6 -0.47813962960358936 1104 7 -0.33768038491174934 1104 8 -0.30027601450917984 1104 9 -0.4796039985764651 1104 10-11 -0.48603098698166747 1104 12-13 -0.5811595608243465 1104 14-15 -0.559393938922959 1104 16-17 -0.5625537434653936 1104 18-19 -0.7240592406130304 1104 20-21 -0.5861611122408021 1104 22-23 -0.5694951591376736 1104 24-25 -0.7011891909073 1104 26-27 -0.5359034473774571 1104 28-29 -0.5906860432849825 1104 30-31 -0.7348332676661684 1104 32-33 -0.7124609444259917 1104 34-35 -0.8584365798689362 1104 36-37 -0.7244554491464896 1104 38-39 -0.6084686596432007 1104 40-41 -0.699161372190332 1104 42-43 -0.7267309874371009 1104 44-45 -0.7363535589029269 1104 46-47 -0.7258293832483886 1104 48-49 -0.5873343378608311 1104 50-51 -0.41719100257692077 1104 52-53 -0.29932261178390007 1104 54-55 -0.307223314732731 1104 56-57 -0.5669897167868072 1104 58-59 -0.6123445649027701 1104 60-61 -0.33634512272232797 1104 62-63 -0.240263450819441 1104 64-65 -0.27577665024528386 1104 66-67 -0.48109488014804214 1104 68-69 -0.3503824537701732 1104 70-71 -0.4473876779567192 1104 72-73 -0.5728508994979293 1104 74-75 -0.5720749987631351 1104 76-77 -0.4166673830419114 1104 78-79 -0.3692094063141411 1104 80-81 -0.7392569600794303 1104 82-83 -0.8343669096794599 1104 84-85 -0.7202566447180274 1104 86-87 -0.7451546109958258 1104 88-89 -0.7523643675916993 1104 90-91 -0.7324420976290789 1104 92-93 -0.9547219939063076 1104 94-95 -0.968339640462883 1104 96-97 -1.027313229310458 1104 98-99 -1.1636756112600999 1104 100 -1.173171654336084 1105 1 -0.4801663896325543 1105 2 -0.46098564600082526 1105 3 -0.4445819642734108 1105 4 -0.3587596555209345 1105 5 -0.3849822433645613 1105 6 -0.3690165778736798 1105 7 -0.1499316539537645 1105 8 -0.30955515776850717 1105 9 -0.44494503685338316 1105 10-11 -0.4906828211210055 1105 12-13 -0.6862423710850081 1105 14-15 -0.7571412137079179 1105 16-17 -0.6697112170818471 1105 18-19 -0.7491222775768378 1105 20-21 -0.563638203062574 1105 22-23 -0.5968648252652997 1105 24-25 -0.5561418397371227 1105 26-27 -0.720961323868444 1105 28-29 -0.7093718088104666 1105 30-31 -0.7859669111388143 1105 32-33 -0.7444770798449767 1105 34-35 -0.6202046293890433 1105 36-37 -0.7703885111718023 1105 38-39 -0.7004632845764576 1105 40-41 -0.707047443256684 1105 42-43 -0.7978341279169214 1105 44-45 -0.7141346577770804 1105 46-47 -0.6955305101219338 1105 48-49 -0.506519034248754 1105 50-51 -0.6903877111149512 1105 52-53 -0.7098318380450905 1105 54-55 -0.7008841081682036 1105 56-57 -0.6874614657528042 1105 58-59 -0.7082006030391739 1105 60-61 -0.4761870385350946 1105 62-63 -0.682997275164098 1105 64-65 -0.5116610965291031 1105 66-67 -0.5881887419955447 1105 68-69 -0.47908771157666763 1105 70-71 -0.6051382227442588 1105 72-73 -0.5324929364029671 1105 74-75 -0.7990647825597677 1105 76-77 -0.9119184707009751 1105 78-79 -0.7217997337486963 1105 80-81 -0.7739350819738888 1105 82-83 -0.8327155583764387 1105 84-85 -0.7994315821919997 1105 86-87 -0.9212475710379309 1105 88-89 -1.0459983367872532 1105 90-91 -1.0248889026066337 1105 92-93 -1.0672090607906348 1105 94-95 -1.0146599934187854 1105 96-97 -1.0763550930075532 1105 98-99 -1.082348858806963 1105 100 -1.1806328805359563 1106 1 -0.5386094952871723 1106 2 -0.45998121128805636 1106 3 -0.3340330376217935 1106 4 -0.3855250676370048 1106 5 -0.40585302047659155 1106 6 -0.33852304918566745 1106 7 -0.48797579978371886 1106 8 -0.47911873191283405 1106 9 -0.4992712953238083 1106 10-11 -0.5505855922516218 1106 12-13 -0.512398908845789 1106 14-15 -0.6236882485714474 1106 16-17 -0.7634368893239056 1106 18-19 -0.809330846698991 1106 20-21 -0.7567030332638041 1106 22-23 -0.6117053994979571 1106 24-25 -0.6563413915415097 1106 26-27 -0.4916893438996155 1106 28-29 -0.7294096651126267 1106 30-31 -0.7830876343318067 1106 32-33 -0.7734362266711443 1106 34-35 -0.7391710886642429 1106 36-37 -0.712422974168959 1106 38-39 -0.577580762356682 1106 40-41 -0.5661508260859875 1106 42-43 -0.5152482836679795 1106 44-45 -0.5419832264062663 1106 46-47 -0.7686126354975684 1106 48-49 -0.7236083919651364 1106 50-51 -0.6134657797246135 1106 52-53 -0.420079896446083 1106 54-55 -0.7482227156396348 1106 56-57 -0.5965598674897166 1106 58-59 -0.6053739101353273 1106 60-61 -0.780969811555444 1106 62-63 -0.7211632266759906 1106 64-65 -0.6362664604980353 1106 66-67 -0.5827457431519818 1106 68-69 -0.5369805862523833 1106 70-71 -0.5988404787169443 1106 72-73 -0.6161105856970934 1106 74-75 -0.4675841246036754 1106 76-77 -0.6020185239010942 1106 78-79 -0.5157971579590637 1106 80-81 -0.557423941786432 1106 82-83 -0.5530464775060935 1106 84-85 -0.5612529468675298 1106 86-87 -0.8052312535954798 1106 88-89 -0.7480614847144018 1106 90-91 -0.8241549874269367 1106 92-93 -0.9008012911081273 1106 94-95 -1.0674559781448032 1106 96-97 -1.0322087495401782 1106 98-99 -1.045268734084516 1106 100 -1.0699406832888059 1107 1 -0.5508056634870542 1107 2 -0.4859997034477992 1107 3 -0.4413407944237626 1107 4 -0.37245927657266975 1107 5 -0.2585660098344391 1107 6 -0.40259241623281383 1107 7 -0.5058001437644108 1107 8 -0.4111984564412694 1107 9 -0.5721275579733103 1107 10-11 -0.539921500656547 1107 12-13 -0.4649796024382695 1107 14-15 -0.5843831474339254 1107 16-17 -0.5892266667706494 1107 18-19 -0.6457072101155532 1107 20-21 -0.5880834523206389 1107 22-23 -0.6056648352960039 1107 24-25 -0.7294267588694296 1107 26-27 -0.7281653434784587 1107 28-29 -0.7668395200844955 1107 30-31 -0.7409616209091965 1107 32-33 -0.7001763498222218 1107 34-35 -0.6630820127652797 1107 36-37 -0.632554158532912 1107 38-39 -0.6708994849859735 1107 40-41 -0.6724212748971752 1107 42-43 -0.8534131259464672 1107 44-45 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0.7868322307929176 2310 88-89 0.7180212377581832 2310 90-91 0.5290563026980735 2310 92-93 0.632735608749389 2310 94-95 0.7478537395376001 2310 96-97 0.8333793207845623 2310 98-99 1.0258772019991405 2310 100 1.0750831386686563 2311 1 0.5573777153086468 2311 2 0.4560029418205005 2311 3 0.5069581086757218 2311 4 0.44118370995356315 2311 5 0.402279895650679 2311 6 0.15762256527169427 2311 7 0.3339564671154065 2311 8 0.4174845298405998 2311 9 0.5479213458898187 2311 10-11 0.6460937814188554 2311 12-13 0.6543048921963575 2311 14-15 0.7694249239944284 2311 16-17 0.7578533052493768 2311 18-19 0.6559056857201284 2311 20-21 0.7352669968556924 2311 22-23 0.8265146550163891 2311 24-25 0.7783918264313101 2311 26-27 0.9259089348000984 2311 28-29 0.876118369443482 2311 30-31 0.9465882069650817 2311 32-33 0.9883131439418449 2311 34-35 0.9423720967170652 2311 36-37 0.9701748816867735 2311 38-39 1.0233520965240288 2311 40-41 1.0023279438933912 2311 42-43 1.03041805339722 2311 44-45 1.01595778360538 2311 46-47 1.0749968908521197 2311 48-49 1.0736574337841134 2311 50-51 1.168255650659006 2311 52-53 1.1329886498162978 2311 54-55 1.0735330952124968 2311 56-57 0.9532739222982798 2311 58-59 0.9820946205143457 2311 60-61 0.9502502234430281 2311 62-63 1.0244343169257348 2311 64-65 1.0615472734745524 2311 66-67 1.0475617620034683 2311 68-69 1.0457659155268146 2311 70-71 1.135981861757024 2311 72-73 1.051202273313713 2311 74-75 1.1695352344949512 2311 76-77 1.1491020126553977 2311 78-79 1.0693872020177828 2311 80-81 1.1447841169693618 2311 82-83 1.2881959356970434 2311 84-85 1.2914477251291387 2311 86-87 1.2290605803114047 2311 88-89 1.2707656436545278 2311 90-91 1.3181588473776777 2311 92-93 1.354258199787715 2311 94-95 1.2667216878865126 2311 96-97 1.4456155230055394 2311 98-99 1.4583433293290717 2311 100 1.3233383742244804 2312 1 0.4558421076278947 2312 2 0.4676711095884656 2312 3 0.4959256457285015 2312 4 0.3797126643659823 2312 5 0.387260145536672 2312 6 0.4143519413398806 2312 7 0.45591894758319285 2312 8 0.34498796889946703 2312 9 0.5207875188141671 2312 10-11 0.574237820194007 2312 12-13 0.6157470105302991 2312 14-15 0.6954846846687772 2312 16-17 0.6381990048783805 2312 18-19 0.6116941997056315 2312 20-21 0.680141314886221 2312 22-23 0.7404754971152698 2312 24-25 0.7974511285644752 2312 26-27 0.857675827874111 2312 28-29 0.7502259194566889 2312 30-31 0.7445821840329714 2312 32-33 0.6868041581768338 2312 34-35 0.8683300119842059 2312 36-37 0.803098991226328 2312 38-39 0.7886469081873884 2312 40-41 0.7825712928356552 2312 42-43 0.7776743854972565 2312 44-45 0.7222266289532087 2312 46-47 0.8653606731726384 2312 48-49 0.8011993372479296 2312 50-51 0.8329745699671207 2312 52-53 0.8029282120673713 2312 54-55 0.7646857136342291 2312 56-57 0.7326346110448156 2312 58-59 0.7576526070259106 2312 60-61 0.790844476393552 2312 62-63 0.8139490717198896 2312 64-65 0.7777499145706983 2312 66-67 0.9006165241999682 2312 68-69 0.9727287054783247 2312 70-71 0.9043880900852699 2312 72-73 0.9545631401712953 2312 74-75 0.9293986069074478 2312 76-77 0.9750357376645837 2312 78-79 0.9766063518596901 2312 80-81 0.8953167131840623 2312 82-83 1.0327430220657412 2312 84-85 1.034058169762499 2312 86-87 1.1063491808260295 2312 88-89 1.0459263541280883 2312 90-91 1.2063712757385154 2312 92-93 1.2147528874049556 2312 94-95 1.2404542573213995 2312 96-97 1.2723616427744737 2312 98-99 1.3771583288048426 2312 100 1.3596000761828186 2313 1 0.4436925538204335 2313 2 0.424414610955381 2313 3 0.44580873627273476 2313 4 0.3395891965748916 2313 5 0.3506127736826272 2313 6 0.29838138493879285 2313 7 0.28540935779993504 2313 8 0.38688468224675177 2313 9 0.5228090781534362 2313 10-11 0.6160145350145427 2313 12-13 0.5856166873778292 2313 14-15 0.6980450492741994 2313 16-17 0.7081331957853365 2313 18-19 0.7438333694821679 2313 20-21 0.574117864085018 2313 22-23 0.48047002589531473 2313 24-25 0.44309830081701307 2313 26-27 0.5683559640783713 2313 28-29 0.6969740484895581 2313 30-31 0.7193131378311648 2313 32-33 0.7442326610348147 2313 34-35 0.7436785720612988 2313 36-37 0.7955380444498275 2313 38-39 0.7244018058500359 2313 40-41 0.830637385885602 2313 42-43 0.685455963228371 2313 44-45 0.8706100052365073 2313 46-47 1.0120658522377681 2313 48-49 0.9599860511536136 2313 50-51 0.9128417577363024 2313 52-53 0.7759604738495014 2313 54-55 0.809504420810498 2313 56-57 0.8144333592582029 2313 58-59 0.8945779061608548 2313 60-61 0.9091814393852857 2313 62-63 0.9441137607870687 2313 64-65 1.0129684128782444 2313 66-67 0.9749783340153684 2313 68-69 0.9754913448280931 2313 70-71 0.8998493834317642 2313 72-73 0.9563599992989609 2313 74-75 0.8592413494321889 2313 76-77 0.8987898915739763 2313 78-79 1.0387597967084865 2313 80-81 1.0979899179882793 2313 82-83 1.1165976361279029 2313 84-85 1.1752242584164598 2313 86-87 1.2996922031493199 2313 88-89 1.452215227413209 2313 90-91 1.5135370590496855 2313 92-93 1.525395806244365 2313 94-95 1.517968374969854 2313 96-97 1.7167151477413434 2313 98-99 1.7334250439461591 2313 100 1.687371002559562 2314 1 0.36754191580432405 2314 2 0.2838567183237757 2314 3 0.39060167058187645 2314 4 0.2811050455662638 2314 5 0.26565007838799914 2314 6 0.3193741093065654 2314 7 0.41106570705740353 2314 8 0.42663851525861674 2314 9 0.5442941703954389 2314 10-11 0.5658026162656924 2314 12-13 0.5815139042422928 2314 14-15 0.685903929805356 2314 16-17 0.6749730810294423 2314 18-19 0.6833978258888251 2314 20-21 0.6927256974516851 2314 22-23 0.6886590104141135 2314 24-25 0.642390765639405 2314 26-27 0.7162305902121204 2314 28-29 0.6965806909078864 2314 30-31 0.7410579731944225 2314 32-33 0.8174338940166237 2314 34-35 0.8767871054208385 2314 36-37 0.7983666880003497 2314 38-39 0.7984543013613816 2314 40-41 0.986771815026998 2314 42-43 0.8302986992909425 2314 44-45 0.7680230541433559 2314 46-47 0.6086909995526568 2314 48-49 0.6625188785033984 2314 50-51 0.8919647101582555 2314 52-53 0.9051005447414369 2314 54-55 0.8069232323706075 2314 56-57 0.774908761203271 2314 58-59 0.7972327096713272 2314 60-61 0.49827883839478915 2314 62-63 0.7345044517291939 2314 64-65 0.6957637290840708 2314 66-67 0.8443241542273725 2314 68-69 0.812632266558829 2314 70-71 0.9554314453091095 2314 72-73 0.9953473146821921 2314 74-75 1.08000944331598 2314 76-77 1.1521696594633468 2314 78-79 1.1639753312397723 2314 80-81 1.229992106666586 2314 82-83 1.2718966217879384 2314 84-85 1.3907133099402316 2314 86-87 1.428190472658443 2314 88-89 1.508896006930705 2314 90-91 1.6038679200917798 2314 92-93 1.909529752904799 2314 94-95 1.889302169315446 2314 96-97 1.9289151084197087 2314 98-99 1.9695797045202355 2314 100 2.039803294208575 2315 1 0.2905444690351189 2315 2 0.29875661232401285 2315 3 0.3651446840539627 2315 4 0.17857364148724741 2315 5 0.25025482242715924 2315 6 0.27140234438853383 2315 7 0.30362933990237195 2315 8 0.2831380380397732 2315 9 0.2743312483206566 2315 10-11 0.30455855950963695 2315 12-13 0.1552318459116364 2315 14-15 0.2963182415326244 2315 16-17 0.17130556072014969 2315 18-19 0.31129577582702694 2315 20-21 0.266428382412478 2315 22-23 0.22714055282061452 2315 24-25 0.39067390485976006 2315 26-27 0.40290823987631086 2315 28-29 0.4434548524761439 2315 30-31 0.4355172314599898 2315 32-33 0.3664076535775038 2315 34-35 0.18931198347713263 2315 36-37 0.28227553370252423 2315 38-39 0.34759176128159197 2315 40-41 0.48290155917612054 2315 42-43 0.5574297178563015 2315 44-45 0.5804825070350361 2315 46-47 0.5157797061796217 2315 48-49 0.39840779756972466 2315 50-51 0.2812151631862889 2315 52-53 -0.11520874585185226 2315 54-55 0.08400883817464688 2315 56-57 0.10847978764163457 2315 58-59 0.14689241634667383 2315 60-61 0.30752558582946676 2315 62-63 0.40144912952950307 2315 64-65 0.4456063513246704 2315 66-67 0.3834967842970016 2315 68-69 0.32354246254843844 2315 70-71 0.2794142489368525 2315 72-73 0.40704464687489406 2315 74-75 0.20585199848203928 2315 76-77 0.5801710526344976 2315 78-79 0.7888053082804909 2315 80-81 0.7689434457382767 2315 82-83 0.786075601947072 2315 84-85 0.8928891090069051 2315 86-87 0.953379298480165 2315 88-89 0.984274565729784 2315 90-91 1.334813115795491 2315 92-93 1.446990882659943 2315 94-95 1.4500673028707496 2315 96-97 1.5165980239849581 2315 98-99 1.8071647572342258 2315 100 1.8411463793734448 2316 1 0.16680180214007123 2316 2 0.19943853755846064 2316 3 0.2615359399953867 2316 4 0.15264817152621646 2316 5 0.07389418353708521 2316 6 0.08392696649276132 2316 7 0.13214708922794216 2316 8 0.12252377006563364 2316 9 0.24353006645965536 2316 10-11 0.2027520464278112 2316 12-13 0.29220942909152825 2316 14-15 0.11689536224516672 2316 16-17 0.27384939877094183 2316 18-19 0.3612101337253719 2316 20-21 0.42176040613534127 2316 22-23 0.339940835439549 2316 24-25 0.4383682589042053 2316 26-27 0.2675377842603268 2316 28-29 0.3186320245739509 2316 30-31 -0.2871110175872431 2316 32-33 -0.12100831011024837 2316 34-35 -0.13446681367954483 2316 36-37 0.14600530075600204 2316 38-39 0.17604394087307895 2316 40-41 0.4926246140298858 2316 42-43 0.4711723312250271 2316 44-45 0.23781213277905522 2316 46-47 0.13919559493053413 2316 48-49 0.21265730512763525 2316 50-51 0.3892652531104943 2316 52-53 -0.03855692381613807 2316 54-55 0.23443731631449793 2316 56-57 0.1851348212561561 2316 58-59 -0.0754288592924297 2316 60-61 0.33700065327261797 2316 62-63 0.34638018763766354 2316 64-65 -0.07866167633772392 2316 66-67 -0.24072494310419046 2316 68-69 -0.2611862125682549 2316 70-71 0.04915716205293563 2316 72-73 -0.10932182638335775 2316 74-75 0.05987664199678733 2316 76-77 0.4683104244496832 2316 78-79 0.45711425145695017 2316 80-81 0.4615155992089228 2316 82-83 0.7824408976264259 2316 84-85 0.9641278149547503 2316 86-87 1.1833585837968243 2316 88-89 1.324635279556702 2316 90-91 0.8848160597427359 2316 92-93 0.8766669414104236 2316 94-95 1.5339408951287048 2316 96-97 1.6590165569357573 2316 98-99 1.7722548955462187 2316 100 1.5453883397211534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 45.0 1 109.0 2 74.0 3 62.0 4 60.0 5 57.0 6 71.0 7 133.0 8 389.0 9 738.0 10 1074.0 11 1377.0 12 1700.0 13 1801.0 14 2074.0 15 2985.0 16 4535.0 17 6766.0 18 9039.0 19 11438.0 20 13895.0 21 16509.0 22 17334.0 23 16922.0 24 16449.0 25 17292.0 26 19333.0 27 22466.0 28 26774.0 29 31926.0 30 38509.0 31 47949.0 32 59485.0 33 75584.0 34 100331.0 35 147801.0 36 198694.0 37 290120.0 38 290228.0 39 59611.0 40 19.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.46400892645921 21.520256964449512 14.645826229273604 22.36990787981768 2 15.980517385975098 23.13534610670279 39.32668860073544 21.55744790658667 3 18.04358671257546 28.607421807020643 30.76316708798426 22.585824392419635 4 13.200916809112401 19.381708162849637 38.24726001438638 29.17011501365159 5 13.204584626213222 37.20432422398201 35.115756526492056 14.475334623312703 6 27.35690610157012 38.17451275832078 19.764130476129687 14.704450663979415 7 24.725017536982403 33.44831232334069 25.078400627205017 16.74826951247189 8 23.576102583277997 34.13872467169096 23.538452611966758 18.746720133064283 9 25.16459423725164 18.367898176876675 23.421084694234736 33.046422891636944 10-11 22.83734391978161 27.706682491326685 29.482895280999998 19.97307830789171 12-13 23.930410352383575 26.344606264009506 29.159594089399093 20.565389294207826 14-15 21.92877052836776 27.868073086017375 27.6791308787392 22.524025506875667 16-17 20.2604972185135 29.96894560097064 28.97784158710736 20.7927155934085 18-19 19.91386407401462 28.951399378397873 31.466689617336264 19.66804693025124 20-21 20.54520901494937 28.52276416238153 31.275469430558257 19.65655739211084 22-23 21.04507785166227 27.91031818617023 30.912520966386403 20.132082995781094 24-25 20.533173407105753 28.352437424026984 31.06318698148918 20.05120218737808 26-27 20.16079085163504 28.55720581403109 31.121309204818438 20.160694129515434 28-29 20.088333384377563 28.12102963198184 31.616807199493213 20.173829784147383 30-31 20.536190661329325 28.276173555789175 31.242788738197223 19.944847044684273 32-33 20.032384388442452 28.675721572915485 31.123731187010655 20.168162851631404 34-35 20.391937149247326 28.325648702047886 31.223350533626 20.05906361507879 36-37 20.558511692796973 27.94963202027974 30.719174626854606 20.77268166006868 38-39 19.93480486590898 28.102249077390855 31.253400952653582 20.709545104046587 40-41 19.895247110042764 27.485156890761935 31.954370657326642 20.66522534186866 42-43 19.789686602926345 27.543659196479926 31.41147325769436 21.25518094289937 44-45 19.27231319149156 27.69122541403654 31.68668728534081 21.349774109131097 46-47 19.512688954416227 27.53912887040998 31.50273046103491 21.44545171413888 48-49 19.325006311199697 27.733458772793263 31.408849994631854 21.532684921375186 50-51 18.87902104958748 27.936846401620237 31.24340900730583 21.94072354148646 52-53 18.931009745205987 28.217118997912316 31.08235464811665 21.769516608765045 54-55 19.0703435211832 28.047691091092965 30.778284589468026 22.103680798255816 56-57 19.090132408270115 27.935694780721974 30.597175161278884 22.376997649729024 58-59 18.894139804306594 27.35929318000331 30.933110845538515 22.813456170151582 60-61 19.45339921753909 27.530876774012185 30.256820743208916 22.758903265239805 62-63 19.42532463554868 27.164543266363317 30.556492291860238 22.853639806227765 64-65 19.595327382410822 26.941249900058544 30.249892321143484 23.21353039638715 66-67 19.777800846061545 27.153372350254777 29.51904992485256 23.549776878831118 68-69 19.85284919702513 26.849460105309447 29.69043587485121 23.607254822814213 70-71 20.09479922868428 26.79806287686836 29.528070456550942 23.579067437896413 72-73 20.405552406689868 26.623347732139646 29.323591409206596 23.64750845196389 74-75 20.57865581658802 26.765998917442236 29.220290833430855 23.435054432538887 76-77 20.602544614576164 26.748402966621477 29.371519208728998 23.277533210073358 78-79 21.02109722054743 26.52315974033595 29.20952969905976 23.246213340056862 80-81 21.122460593230315 26.55201687150438 29.092511802927625 23.233010732337682 82-83 21.327755241389383 26.028556073912192 29.348073130732583 23.29561555396585 84-85 21.46468334608454 26.01339860146173 29.07626769733824 23.44565035511549 86-87 21.76123423802167 25.840939747911513 29.165269613975 23.23255640009182 88-89 22.352513302361395 25.251739713179067 29.169661169034377 23.22608581542516 90-91 22.79505416799139 25.077242349766777 28.75143731068289 23.376266171558953 92-93 23.326047755895964 24.659314876250523 28.61877576501788 23.395861602835634 94-95 23.429183206869975 24.087932074181804 28.72816451308114 23.754720205867084 96-97 23.972248036718856 23.860323115092097 28.221461079728854 23.945967768460196 98-99 24.15831232587621 23.806348062793727 28.068746869843203 23.96659274148686 100 25.075061995110083 23.32164734380878 27.85375929442693 23.749531366654214 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7617.0 1 4718.0 2 3556.5 3 4657.0 4 3017.5 5 2199.5 6 2837.0 7 4455.5 8 6050.5 9 5973.5 10 4688.0 11 3648.0 12 3353.5 13 3347.5 14 3354.5 15 3303.0 16 3299.5 17 3332.5 18 3369.5 19 3445.5 20 3614.5 21 3955.5 22 4213.0 23 4462.0 24 4788.5 25 5314.0 26 6474.5 27 8054.5 28 8791.5 29 9604.0 30 11554.0 31 13591.5 32 16707.0 33 21621.5 34 27406.0 35 33116.0 36 40533.0 37 48947.0 38 54333.0 39 57153.0 40 58067.0 41 58606.0 42 59817.0 43 62405.0 44 65971.5 45 68860.5 46 68714.0 47 66134.5 48 63742.5 49 61386.5 50 59081.5 51 54910.5 52 49420.0 53 44847.5 54 40840.0 55 36505.5 56 32159.0 57 27599.5 58 23382.0 59 19592.0 60 15808.5 61 12649.5 62 10026.5 63 7637.0 64 5601.5 65 4018.5 66 2928.0 67 2267.0 68 1774.5 69 1357.0 70 1063.0 71 844.0 72 715.5 73 629.0 74 497.0 75 377.0 76 278.0 77 179.5 78 123.0 79 89.5 80 54.0 81 36.0 82 29.5 83 19.0 84 15.0 85 12.0 86 8.0 87 8.0 88 5.0 89 4.0 90 3.5 91 2.5 92 3.0 93 2.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026743860833970245 2 0.10684655725957269 3 0.017786278530544066 4 0.018688481064702098 5 0.02010622790409329 6 0.047107860890680114 7 0.048332278615608876 8 0.040470227960803164 9 0.03344593680200134 10-11 0.03818250010633101 12-13 0.03731251909125005 14-15 0.031577088695531136 16-17 0.03924581023587441 18-19 0.03898803808325783 20-21 0.04875115836361082 22-23 0.04920225963068983 24-25 0.0672140887947734 26-27 0.05951314573535307 28-29 0.05255329761470538 30-31 0.051457765966084916 32-33 0.04523901278420991 34-35 0.0463023229137533 36-37 0.04211352543373387 38-39 0.04652787354729281 40-41 0.05606544319410629 42-43 0.05683875965195603 44-45 0.057902069781499435 46-47 0.0593520381399677 48-49 0.06080200649843597 50-51 0.05622655078949166 52-53 0.06415304448245152 54-55 0.05004001912669372 56-57 0.05625877230856874 58-59 0.05229552546208881 60-61 0.04859005076822546 62-63 0.055743228003335574 64-65 0.054776582431023395 66-67 0.052005531790395146 68-69 0.05799873433873066 70-71 0.05809539889596187 72-73 0.06025424067412573 74-75 0.05165109508054735 76-77 0.05384215837778829 78-79 0.06347639258183299 80-81 0.0629930697956769 82-83 0.05216663938578051 84-85 0.05957758877350721 86-87 0.05632321534672288 88-89 0.06441081663506809 90-91 0.07211175969448844 92-93 0.07771830401389908 94-95 0.08010269642560244 96-97 0.07507613944957912 98-99 0.08483925972993211 100 0.13139935479630202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1551758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.12122792037796 #Duplication Level Percentage of deduplicated Percentage of total 1 81.79042437003253 38.54065228444738 2 10.494990217893479 9.890736521589915 3 2.8308890075749518 4.001848984296956 4 1.2502405172547213 2.3565147347540383 5 0.6876597034039869 1.6201684807879386 6 0.43887597677593315 1.240822495826235 7 0.33295836428703124 1.0982584880095803 8 0.25423897784475497 0.9584042257013297 9 0.19166278249966254 0.8128247092218386 >10 1.3384357923910524 13.061651406661834 >50 0.2204412955384186 7.299668127443305 >100 0.162782271164863 13.690635873078854 >500 0.004509600534049546 1.397413705461204 >1k 0.0011637678797547216 1.1903310603028454 >5k 5.818839398773608E-4 2.0647257050032826 >10k+ 1.454709849693402E-4 0.7753431974135032 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11311 0.728915204561536 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9759 0.6288996093463027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7678 0.4947936469475266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6457 0.41610869736131534 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6227 0.4012867985858619 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4759 0.3066844185755769 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3342 0.21536863351115315 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2317 0.14931451940315435 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.139390291527416 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.444303815414517E-5 0.0 14-15 0.0 0.0 0.0 1.6110759538536293E-4 0.0 16-17 0.0 0.0 0.0 3.5443670984779845E-4 6.444303815414517E-5 18-19 0.0 0.0 0.0 5.155443052331614E-4 6.444303815414517E-5 20-21 0.0 0.0 0.0 0.0013533038012370484 6.444303815414517E-5 22-23 0.0 0.0 0.0 0.005155443052331614 9.666455723121776E-5 24-25 0.0 0.0 0.0 0.017528506377927487 1.2888607630829035E-4 26-27 0.0 0.0 0.0 0.03769917732017493 1.2888607630829035E-4 28-29 0.0 0.0 0.0 0.07894272173882783 1.2888607630829035E-4 30-31 0.0 0.0 0.0 0.21736636769393164 1.2888607630829035E-4 32-33 0.0 0.0 0.0 0.5864638687217981 1.2888607630829035E-4 34-35 0.0 0.0 0.0 1.1573969652484473 2.577721526165807E-4 36-37 0.0 3.222151907707259E-5 0.0 1.962580505465414 2.577721526165807E-4 38-39 0.0 6.444303815414517E-5 0.0 3.053536698376938 2.577721526165807E-4 40-41 0.0 6.444303815414517E-5 0.0 4.205874885130284 3.2221519077072587E-4 42-43 0.0 6.444303815414517E-5 0.0 5.348063293374354 3.2221519077072587E-4 44-45 0.0 6.444303815414517E-5 0.0 6.669918891992179 3.2221519077072587E-4 46-47 0.0 6.444303815414517E-5 0.0 8.069041693356825 3.2221519077072587E-4 48-49 0.0 6.444303815414517E-5 0.0 9.32310321583649 3.8665822892487104E-4 50-51 0.0 6.444303815414517E-5 0.0 10.514075003963246 4.1887974800194363E-4 52-53 0.0 6.444303815414517E-5 0.0 11.663674361595042 4.5110126707901616E-4 54-55 0.0 6.444303815414517E-5 0.0 12.90471839036757 5.155443052331614E-4 56-57 0.0 6.444303815414517E-5 0.0 14.26517536883973 5.155443052331614E-4 58-59 0.0 6.444303815414517E-5 0.0 15.624246821991573 5.155443052331614E-4 60-61 0.0 6.444303815414517E-5 0.0 16.960344332041466 5.155443052331614E-4 62-63 0.0 6.444303815414517E-5 0.0 18.262190367312428 5.155443052331614E-4 64-65 0.0 6.444303815414517E-5 0.0 19.671495168705427 5.155443052331614E-4 66-67 0.0 6.444303815414517E-5 0.0 21.162707071592347 5.155443052331614E-4 68-69 0.0 6.444303815414517E-5 0.0 22.68804156318189 5.155443052331614E-4 70-71 0.0 6.444303815414517E-5 0.0 24.290901029670863 5.155443052331614E-4 72-73 0.0 6.444303815414517E-5 0.0 25.835665097263877 5.155443052331614E-4 74-75 0.0 6.444303815414517E-5 0.0 27.404305310493 5.155443052331614E-4 76-77 0.0 6.444303815414517E-5 0.0 28.998174973159472 5.155443052331614E-4 78-79 0.0 6.444303815414517E-5 0.0 30.636091452404305 5.155443052331614E-4 80-81 0.0 6.444303815414517E-5 0.0 32.27684342532792 5.155443052331614E-4 82-83 0.0 6.444303815414517E-5 0.0 33.872743043696246 6.444303815414517E-4 84-85 0.0 6.444303815414517E-5 0.0 35.437130016407195 6.444303815414517E-4 86-87 0.0 1.2888607630829035E-4 0.0 36.998198172653204 6.444303815414517E-4 88 0.0 1.2888607630829035E-4 0.0 38.15536958726812 6.444303815414517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4920 0.0 35.7515 1 GTATCAA 8070 0.0 33.801983 1 TACCTGG 780 0.0 31.957222 2 ATCAACG 9730 0.0 27.527802 3 TCAACGC 9870 0.0 27.184946 4 CAACGCA 9870 0.0 27.137337 5 TATCAAC 10165 0.0 26.419006 2 AACGCAG 10315 0.0 26.10327 6 CCGTATC 625 0.0 24.826162 94 ACGCAGA 11225 0.0 23.863054 7 CGCAGAG 11320 0.0 23.581282 8 GCAGAGT 11665 0.0 22.321972 9 CGTATCA 530 0.0 22.178888 94 GTGGTAT 3295 0.0 21.12487 1 ACCTGGG 1140 0.0 21.02204 3 GAGTACT 6985 0.0 19.748869 12-13 GCGGTAT 310 2.9649527E-10 19.722837 1 GTACATG 6820 0.0 19.584917 1 TGGTATC 3520 0.0 19.507336 2 CGCCGTT 170 8.602795E-5 19.360865 94 >>END_MODULE