FastQCFastQC Report
Mon 6 Jun 2016
ERR1211364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551758
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97610.629028495422611No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93530.6027357358557197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68570.4418859126229734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62320.4016090137766327No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.325372899640279No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44370.2859337602899421No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.18153603848022695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.14757455737299244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.12901496238459864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45100.037.1578641
GTATCAA73700.034.203131
TATCACG550.002741234434.120732
TACCTGG7850.030.4804612
ATCAACG93600.026.8707623
CAACGCA94900.026.3978235
TCAACGC95650.026.3439184
CATATAG2900.025.8888653
TATCAAC97850.025.843362
AACGCAG98300.025.436236
ACCTGGG9900.024.6467233
GTACGCA1351.4719446E-524.3401551
ACCGTCC1351.4793144E-524.3244658
GTACACG1401.9505105E-523.4708631
ACGCAGA109450.022.8449657
GACCGAC1252.4550932E-422.5175047
CGCAGAG111250.022.4331688
GTGGTAT30250.022.0354561
GTATAGT5050.021.37941
GCAGAGT116700.021.144339
Produced by FastQC (version 0.11.5)