##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211364_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1551758 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02700936615116 33.0 31.0 34.0 30.0 34.0 2 32.15692137562687 33.0 31.0 34.0 30.0 34.0 3 32.22194762327631 34.0 31.0 34.0 30.0 34.0 4 35.89016199690931 37.0 35.0 37.0 35.0 37.0 5 35.7778390702674 37.0 35.0 37.0 35.0 37.0 6 35.80055266349521 37.0 35.0 37.0 35.0 37.0 7 35.719356368712134 37.0 35.0 37.0 33.0 37.0 8 35.70892497412612 37.0 35.0 37.0 33.0 37.0 9 37.307259894906295 39.0 37.0 39.0 34.0 39.0 10-11 37.27023833613231 39.0 37.0 39.0 34.0 39.0 12-13 37.21905219757205 39.0 37.0 39.0 33.0 39.0 14-15 38.41634745881768 40.0 38.0 41.0 33.5 41.0 16-17 38.39402084603398 40.0 38.0 41.0 33.5 41.0 18-19 38.39167479722998 40.0 38.0 41.0 33.5 41.0 20-21 38.36672309728708 40.0 38.0 41.0 34.0 41.0 22-23 38.23181385241771 40.0 37.5 41.0 33.5 41.0 24-25 38.12101371476738 40.0 37.0 41.0 33.0 41.0 26-27 37.99513261732822 40.0 37.0 41.0 33.0 41.0 28-29 37.85693097763955 40.0 37.0 41.0 33.0 41.0 30-31 37.812387305237024 40.0 37.0 41.0 33.0 41.0 32-33 37.68938004508435 40.0 36.0 41.0 33.0 41.0 34-35 37.5107004442703 40.0 36.0 41.0 32.5 41.0 36-37 37.3276064953427 40.0 36.0 41.0 32.0 41.0 38-39 37.195003022378486 40.0 35.0 41.0 31.5 41.0 40-41 37.08103164282059 39.0 35.0 41.0 31.0 41.0 42-43 36.94083549110106 39.0 35.0 41.0 31.0 41.0 44-45 36.769726336194175 39.0 35.0 41.0 30.5 41.0 46-47 36.50462797678504 39.0 35.0 41.0 30.0 41.0 48-49 34.935967141783706 39.0 35.0 40.5 20.5 41.0 50-51 35.67317423206454 38.5 34.5 40.0 27.0 41.0 52-53 35.78842126156269 39.0 35.0 40.0 27.5 41.0 54-55 35.62290608458278 38.0 35.0 40.0 27.0 41.0 56-57 35.41455980893928 38.0 34.5 40.0 26.5 41.0 58-59 35.19280454813186 38.0 34.0 40.0 26.0 41.0 60-61 34.93265476962259 38.0 34.0 40.0 26.0 41.0 62-63 34.59907601571895 37.0 34.0 40.0 25.5 41.0 64-65 34.250568387596516 36.5 33.0 39.5 25.0 41.0 66-67 33.883795991385256 36.0 33.0 39.0 23.5 40.5 68-69 33.463165970467045 35.5 33.0 39.0 23.0 40.0 70-71 33.05845080225138 35.0 32.0 38.0 22.5 40.0 72-73 32.64252770083995 35.0 32.0 37.0 22.0 39.5 74-75 32.229825140260274 35.0 31.0 37.0 21.0 39.0 76-77 31.173659488141837 34.0 30.0 35.5 20.0 38.0 78-79 31.562221686628973 34.5 31.0 36.0 20.0 37.5 80-81 31.386401745633016 34.0 31.0 35.0 20.0 37.0 82-83 31.088735808031924 34.0 31.0 35.0 19.5 37.0 84-85 30.79000527144052 34.0 30.0 35.0 18.5 36.0 86-87 30.502364415069877 34.0 30.0 35.0 18.0 36.0 88-89 30.238228512435576 34.0 30.0 35.0 17.0 35.0 90-91 30.01049358211783 34.0 29.0 35.0 16.5 35.0 92-93 29.812299340489947 34.0 29.0 35.0 15.0 35.0 94-95 29.61189663594452 34.0 29.0 35.0 15.0 35.0 96-97 29.40590543113037 33.0 29.0 35.0 13.5 35.0 98-99 29.144928526226384 33.0 27.5 35.0 10.5 35.0 100 26.547761957727946 30.0 23.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2861106840610077 1101 2 -0.21833122246783887 1101 3 -0.21019611040942365 1101 4 -0.12344158390128968 1101 5 -0.07008007303911512 1101 6 -0.11231806088793661 1101 7 -0.12751327052394146 1101 8 -0.04869958505793193 1101 9 -0.2148827349600282 1101 10-11 -0.11826131021538089 1101 12-13 -0.2108456871968869 1101 14-15 -0.34864856300953306 1101 16-17 -0.4403821803721044 1101 18-19 -0.3447544264424707 1101 20-21 -0.3809208327355904 1101 22-23 -0.4663971082730498 1101 24-25 -0.4533426226424382 1101 26-27 -0.38838477952763384 1101 28-29 -0.5371812808051359 1101 30-31 -0.39095792602626034 1101 32-33 -0.32946489742967344 1101 34-35 -0.2922822603932289 1101 36-37 -0.402632787271358 1101 38-39 -0.3673796792124264 1101 40-41 -0.3214974470548597 1101 42-43 -0.46900857437324817 1101 44-45 -0.5523970925845489 1101 46-47 -0.4829819574804972 1101 48-49 0.6691655495619244 1101 50-51 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1105 68-69 -0.9103524854511065 1105 70-71 -0.8393848021223107 1105 72-73 -0.8036432450546798 1105 74-75 -0.770931502783732 1105 76-77 -0.9320546361364634 1105 78-79 -0.7788570033440543 1105 80-81 -0.7078054516433205 1105 82-83 -0.639061896330805 1105 84-85 -0.7323641249041088 1105 86-87 -0.615520838607118 1105 88-89 -0.8676009059624121 1105 90-91 -0.7215416237049936 1105 92-93 -0.7466461810381162 1105 94-95 -0.6186661160446825 1105 96-97 -0.7889393651716894 1105 98-99 -0.6682197851789979 1105 100 -1.078259873531909 1106 1 -0.258227575713768 1106 2 -0.27176963357726436 1106 3 -0.2784819185898151 1106 4 -0.21862671291017222 1106 5 -0.27801315020850836 1106 6 -0.2602106068541943 1106 7 -0.3237378015922445 1106 8 -0.3705645259885486 1106 9 -0.47507030763811997 1106 10-11 -0.5128949283625701 1106 12-13 -0.45204953134135195 1106 14-15 -0.5656706150175594 1106 16-17 -0.5453124936673532 1106 18-19 -0.560600989295331 1106 20-21 -0.6030608971136289 1106 22-23 -0.5325131067225826 1106 24-25 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0.5909030657667493 2310 92-93 0.6123049958104723 2310 94-95 0.7445729721348506 2310 96-97 0.813633660670984 2310 98-99 0.796517610398709 2310 100 0.943395543670924 2311 1 0.33983482304762447 2311 2 0.39223040545448384 2311 3 0.3814685761376424 2311 4 0.298475699902653 2311 5 0.3278068093285782 2311 6 0.3266480526653268 2311 7 0.4348654471506066 2311 8 0.21906538794644348 2311 9 0.4177159740019931 2311 10-11 0.4020662675703264 2311 12-13 0.3773727661976878 2311 14-15 0.3629329915163666 2311 16-17 0.49218319657666143 2311 18-19 0.560482824887508 2311 20-21 0.6256399635365071 2311 22-23 0.6545205451532183 2311 24-25 0.5901548296036765 2311 26-27 0.6676633307782254 2311 28-29 0.699238398943514 2311 30-31 0.6338991753426626 2311 32-33 0.6697463549299698 2311 34-35 0.802434285486143 2311 36-37 0.7688356694234244 2311 38-39 0.8803605092949667 2311 40-41 0.8303183226176287 2311 42-43 0.8954934915515338 2311 44-45 0.8923674711951008 2311 46-47 0.9369774830697111 2311 48-49 -5.988720111732015 2311 50-51 -0.880916197168446 2311 52-53 0.6570290308256688 2311 54-55 0.9619010059152728 2311 56-57 1.163558900769587 2311 58-59 1.2170026831539005 2311 60-61 1.0599838766426757 2311 62-63 0.9304692540113848 2311 64-65 0.94898660917341 2311 66-67 1.1947500843830312 2311 68-69 1.2393730301724588 2311 70-71 1.2350091521420836 2311 72-73 0.7702294141454544 2311 74-75 1.0140010989130488 2311 76-77 1.0336161129355794 2311 78-79 0.8573812091930897 2311 80-81 0.9664692000623951 2311 82-83 1.0258746955257294 2311 84-85 1.0402997411423662 2311 86-87 1.0858088660406153 2311 88-89 1.1299838809099612 2311 90-91 1.1763428676059462 2311 92-93 1.0775050251002973 2311 94-95 1.250653135349861 2311 96-97 1.45281081864918 2311 98-99 1.2303396545253449 2311 100 1.3539769971531292 2312 1 0.09522773473360502 2312 2 0.2731549659560244 2312 3 0.30888295967766055 2312 4 0.11847207452195363 2312 5 0.2801422113605625 2312 6 0.16182397205302834 2312 7 0.20209800584829907 2312 8 0.2942876162039454 2312 9 0.14388035912100605 2312 10-11 0.16536033015677276 2312 12-13 0.214646700973681 2312 14-15 0.17919282394040437 2312 16-17 0.39392022131388416 2312 18-19 0.2187649565608254 2312 20-21 0.31018320978069625 2312 22-23 0.587360209849713 2312 24-25 0.753077322248707 2312 26-27 0.558988754802499 2312 28-29 0.671474393542077 2312 30-31 0.653984510740905 2312 32-33 0.6981844348171933 2312 34-35 0.28678230434822893 2312 36-37 0.5655364466696753 2312 38-39 0.5907456459920226 2312 40-41 0.47491843175800597 2312 42-43 0.36722010121425086 2312 44-45 0.5614760065034048 2312 46-47 0.6929329919430458 2312 48-49 -5.070639188687352 2312 50-51 -0.9175195944154311 2312 52-53 0.44619530063735624 2312 54-55 0.7086102150138416 2312 56-57 0.9138889114688808 2312 58-59 0.862392449474747 2312 60-61 0.8518012850386469 2312 62-63 0.6117381309391803 2312 64-65 0.79213726079454 2312 66-67 0.7980505387556533 2312 68-69 0.763105062747826 2312 70-71 0.9363384162908659 2312 72-73 0.872971060927263 2312 74-75 0.6495273349215438 2312 76-77 0.7778128195235432 2312 78-79 0.39582603456216603 2312 80-81 0.6788865079077446 2312 82-83 0.9050473571235997 2312 84-85 0.8910180591678376 2312 86-87 0.927078140876052 2312 88-89 0.8905289070013325 2312 90-91 0.9423614922097769 2312 92-93 1.0019886294108353 2312 94-95 1.1728317175607366 2312 96-97 1.1727068952798732 2312 98-99 1.0119006777669384 2312 100 0.8137981155641256 2313 1 0.09717236432428678 2313 2 0.17983710173970735 2313 3 0.2561828146101348 2313 4 -0.03693284921957485 2313 5 0.13683783212719902 2313 6 0.2451706715588955 2313 7 0.1054596873484499 2313 8 0.23000939546130894 2313 9 0.47173029442873116 2313 10-11 0.2036135733842812 2313 12-13 0.00374765656591336 2313 14-15 0.12117814016028206 2313 16-17 0.07655174148136012 2313 18-19 0.3989017548813294 2313 20-21 0.3346712259674334 2313 22-23 0.3875623657836371 2313 24-25 0.07656528140643815 2313 26-27 0.2827353642500654 2313 28-29 0.18073878475153293 2313 30-31 0.38384465899255105 2313 32-33 0.3457269303350756 2313 34-35 0.29917348885249595 2313 36-37 -0.4466321168053895 2313 38-39 0.04063378597921741 2313 40-41 0.2457586709471471 2313 42-43 0.37411169977283976 2313 44-45 0.3987566676121119 2313 46-47 0.2193008860401946 2313 48-49 -5.524233619805226 2313 50-51 -1.5613461454557935 2313 52-53 -0.105837426881628 2313 54-55 -0.051479463093080824 2313 56-57 -0.01103240632880187 2313 58-59 0.16791879746809713 2313 60-61 0.4377971599407786 2313 62-63 0.56804455846423 2313 64-65 0.6920348759129311 2313 66-67 0.8115217100006831 2313 68-69 0.8215854833842542 2313 70-71 0.8325089484546311 2313 72-73 0.4810441400300576 2313 74-75 0.706402478236619 2313 76-77 0.818205838460738 2313 78-79 0.7190762243618174 2313 80-81 -0.10223038729077061 2313 82-83 0.23454890581745502 2313 84-85 0.6207904362198384 2313 86-87 0.8472914471681321 2313 88-89 0.9769061633597715 2313 90-91 0.9625081755574243 2313 92-93 1.0536496288203487 2313 94-95 0.9231057240976881 2313 96-97 1.063776153439477 2313 98-99 1.3371779218879034 2313 100 1.4347426703042458 2314 1 0.07880673188439147 2314 2 0.28602867719261127 2314 3 0.2685708242235947 2314 4 0.08026341658111136 2314 5 0.27597427028771193 2314 6 0.1930717663500232 2314 7 0.19912907376146904 2314 8 0.3378224233514189 2314 9 0.48222425550709147 2314 10-11 0.39875579792663274 2314 12-13 0.2703233354573129 2314 14-15 0.2560401867609343 2314 16-17 0.5718260833242113 2314 18-19 0.5884930471732659 2314 20-21 0.692992440779939 2314 22-23 0.2642718116762808 2314 24-25 0.38083238490482074 2314 26-27 0.5289527755984267 2314 28-29 0.7142383028355326 2314 30-31 0.5596219674718981 2314 32-33 0.5532523622798351 2314 34-35 0.39140715540278137 2314 36-37 0.5553305750994397 2314 38-39 0.7000174758626017 2314 40-41 0.36494107474191395 2314 42-43 0.40066905681666753 2314 44-45 0.6816906547241146 2314 46-47 0.7139509404464164 2314 48-49 -6.2669413210828395 2314 50-51 -1.4048437822029953 2314 52-53 -0.6605089047988031 2314 54-55 -0.26774167059604537 2314 56-57 0.07164302409054812 2314 58-59 0.5876371280161479 2314 60-61 0.804781281006214 2314 62-63 0.5784465713251166 2314 64-65 0.7375263624401853 2314 66-67 1.0274439492095482 2314 68-69 0.996736103321453 2314 70-71 0.763977300753865 2314 72-73 1.0240746346351983 2314 74-75 1.0123632085794725 2314 76-77 0.9515904989557136 2314 78-79 0.866268795284828 2314 80-81 0.772504361169414 2314 82-83 0.7914140054944099 2314 84-85 0.8005799920322652 2314 86-87 0.8821635501636962 2314 88-89 0.8056466055473628 2314 90-91 0.9370366831771229 2314 92-93 1.1651610654272062 2314 94-95 1.0462481158699752 2314 96-97 1.1010672996312074 2314 98-99 1.1310315719637742 2314 100 -0.1057023986159571 2315 1 0.21023736581575037 2315 2 0.21343560852147903 2315 3 0.26254566137541246 2315 4 0.20025591742778914 2315 5 0.24884784247970515 2315 6 0.24821610855772036 2315 7 0.30088857085637244 2315 8 0.2673859110514911 2315 9 0.44452534101503005 2315 10-11 0.24927922673305858 2315 12-13 0.4206819093426688 2315 14-15 0.0873350772205228 2315 16-17 0.2317412670204817 2315 18-19 0.2323639462799676 2315 20-21 0.18324051760997406 2315 22-23 0.31524557453590774 2315 24-25 0.24677910902833844 2315 26-27 0.41761027871024936 2315 28-29 0.32927484746318925 2315 30-31 0.3383023803871552 2315 32-33 0.43850638235381467 2315 34-35 0.4339392852063071 2315 36-37 0.5422345463519562 2315 38-39 0.5242534583836331 2315 40-41 0.4358336389245494 2315 42-43 0.49984865823996216 2315 44-45 0.34163745142008395 2315 46-47 0.23668027489106436 2315 48-49 -5.3519728681075875 2315 50-51 -1.3332503715616681 2315 52-53 -0.19495511651683728 2315 54-55 -0.07988361901242769 2315 56-57 0.2760491065416488 2315 58-59 0.41679910313039414 2315 60-61 0.17113553793028302 2315 62-63 -0.025277742425380723 2315 64-65 0.012243329879346732 2315 66-67 -0.6610847103113002 2315 68-69 0.1382056514809733 2315 70-71 0.1563819064655405 2315 72-73 0.34871732526983834 2315 74-75 0.45110175092879956 2315 76-77 0.39276695337362 2315 78-79 0.4509055921567331 2315 80-81 0.28996147195016064 2315 82-83 0.26164857945013864 2315 84-85 0.41574106438919856 2315 86-87 0.4426650587910217 2315 88-89 0.6422913776918584 2315 90-91 0.7041164996787792 2315 92-93 0.5818459668386282 2315 94-95 -0.028495186572417452 2315 96-97 0.19754234020580697 2315 98-99 0.46741010767162194 2315 100 0.7302805622520978 2316 1 0.1435366207294635 2316 2 0.24143682892142948 2316 3 0.21786724746713304 2316 4 -5.330604337245859E-4 2316 5 0.19651463047268436 2316 6 0.10787707288778137 2316 7 0.2552814754082462 2316 8 0.242889058398724 2316 9 0.40996500615441533 2316 10-11 0.3976629996958465 2316 12-13 0.34041063798566995 2316 14-15 0.4177412348876288 2316 16-17 0.3856146986994915 2316 18-19 0.12691898203554075 2316 20-21 0.3317037771483555 2316 22-23 0.49683653715312914 2316 24-25 0.5438686157298349 2316 26-27 0.5702943557024938 2316 28-29 0.49161988878550034 2316 30-31 0.42569586302322193 2316 32-33 0.37580432052021706 2316 34-35 0.2696268842299858 2316 36-37 0.310606918209956 2316 38-39 0.3188545267688525 2316 40-41 0.03809940777652088 2316 42-43 -0.10303426900706825 2316 44-45 0.17423684978432163 2316 46-47 0.2720384194549297 2316 48-49 -4.962088806102706 2316 50-51 -1.0391829605224032 2316 52-53 0.15188014019536666 2316 54-55 0.45897728750920663 2316 56-57 0.357882810990759 2316 58-59 0.18932458165563304 2316 60-61 0.3264598003376875 2316 62-63 0.18254325487689016 2316 64-65 0.2833808710946002 2316 66-67 0.40524991479040295 2316 68-69 0.018117226011007403 2316 70-71 -0.4420543222845126 2316 72-73 -0.2405929346539466 2316 74-75 0.14449473041751304 2316 76-77 -0.003345430474279709 2316 78-79 0.09918579170282982 2316 80-81 -0.03609808142627813 2316 82-83 -0.15349026780717523 2316 84-85 0.07996862615856415 2316 86-87 0.23769192900041958 2316 88-89 0.08909773019474798 2316 90-91 0.13080900171278032 2316 92-93 0.20697817683306496 2316 94-95 0.5104436007354245 2316 96-97 0.5331831037051451 2316 98-99 0.6012767415088192 2316 100 -0.11574136212592734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 20.0 13 131.0 14 424.0 15 1179.0 16 2563.0 17 4834.0 18 7171.0 19 9511.0 20 12046.0 21 15045.0 22 16731.0 23 16060.0 24 16061.0 25 17220.0 26 20104.0 27 23594.0 28 27922.0 29 33355.0 30 40115.0 31 48921.0 32 59945.0 33 75993.0 34 102112.0 35 147282.0 36 211914.0 37 329718.0 38 281047.0 39 30726.0 40 9.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.70502254553425 22.248365086169386 15.30408269166927 22.7425296766271 2 15.536872441561922 23.523452563851396 39.08945382180382 21.85022117278286 3 17.619985113629408 28.584280379312453 31.117541106682435 22.678193400375704 4 13.047648453025463 19.608568612650775 38.17322995030885 29.170552984014908 5 12.928331744801183 37.5780974604752 35.07775429886444 14.415816495859183 6 27.451703165055374 38.42699699308784 19.48029267450208 14.641007167354703 7 24.87902422866467 33.51890792179446 24.643678101661536 16.95838974787934 8 23.819451515569437 34.496363329367774 23.11009773834274 18.57408741672005 9 24.979166787508966 18.273804805205433 23.234203977935163 33.51282442935044 10-11 22.883768198878165 27.899833993235994 29.262880990678873 19.95351681720696 12-13 23.830791587589538 26.42994544886274 29.035833620642432 20.70342934290529 14-15 21.782122388403078 27.941239492149705 27.51382062842978 22.762817491017437 16-17 20.18801353310778 30.105783792492346 28.82236184952473 20.88384082487514 18-19 19.904456751632665 29.11497797981386 31.280972935212837 19.699592333340636 20-21 20.471014317636616 28.680512257875346 31.11152999099408 19.73694343349396 22-23 20.92306512501337 28.12997420982965 30.70660684618825 20.240353818968728 24-25 20.502423702664977 28.51723657941509 30.8754328961088 20.104906821811134 26-27 20.116854711694366 28.713341530498354 30.977228378688405 20.192575379118882 28-29 20.02608686966264 28.360029734391457 31.39800939397972 20.215874001966185 30-31 20.51958145543257 28.364569293047897 31.058048521293113 20.05780073022642 32-33 19.9301172532336 28.82890489101888 31.024639742613907 20.21633811313361 34-35 20.373344264947256 28.507373844115573 31.065313492711883 20.05396839822529 36-37 20.496644987463018 28.11610083730284 30.578054801760977 20.809199373473163 38-39 19.813375196556144 28.277528415435977 31.142196265763573 20.766900122244305 40-41 19.83461141125194 27.6355161086797 31.72801848836003 20.801853991708334 42-43 19.67952134429866 27.622594621696884 31.334058139602327 21.363825894402126 44-45 19.218947778181658 27.72403796825495 31.578384286793348 21.478629966770043 46-47 19.420244926264964 27.6877148228664 31.34182237464141 21.550217876227226 48-49 19.40116776102812 27.916279473310485 31.103958521357907 21.57859424430349 50-51 18.706972175366925 27.942199221768817 31.09998472413529 22.250843878728972 52-53 18.679381125673718 28.226633974921718 30.943748541698092 22.150236357706472 54-55 18.851517896474263 27.977554847256503 30.611731275592458 22.559195980676776 56-57 18.672456295818897 27.917860307741883 30.26243454442923 23.147248852009987 58-59 18.32206276393998 27.45913512276784 30.46972252969904 23.749079583593144 60-61 18.88545170978178 27.829034711212508 29.692909529149425 23.592604049856284 62-63 18.724728189065694 27.48248615971591 29.856989101783288 23.93579654943511 64-65 18.682883675511576 27.191012322284045 29.57325611562031 24.55284788658407 66-67 18.888317334096552 27.501411697363025 28.79488746386957 24.815383504670848 68-69 18.848863960869224 27.264681292145802 28.904906881985625 24.981547864999346 70-71 18.92269066273894 27.03925252922426 28.597718783136138 25.44033802490065 72-73 18.806243662969568 26.858695743255918 28.49541413319737 25.839646460577143 74-75 18.661932685128466 27.10875772620654 28.357421621358604 25.871887967306396 76-77 18.51865100350394 26.90503651697976 28.483115307805768 26.093197171710532 78-79 18.468941089124225 26.497874496014518 28.44753137341844 26.585653041442814 80-81 18.172708006610765 26.737251093389457 28.378825574212733 26.71121532578704 82-83 18.162626044530093 26.202599361277702 28.560812356795047 27.073962237397158 84-85 18.144042758977655 25.852821995193352 28.39192635524127 27.61120889058772 86-87 18.037321129302565 25.958843625112806 28.651895062524176 27.351940183060464 88-89 18.258446612894126 25.295407846707977 28.708379330072603 27.737766210325294 90-91 18.217623500148733 24.88727448047643 28.612510075281406 28.282591944093426 92-93 18.294904144551758 24.690777961464466 28.687671400627114 28.326646493356666 94-95 18.000901624051128 24.14000114072521 29.100286824720484 28.758810410503177 96-97 18.156485156379144 23.970616619841483 28.96902642177724 28.903871802002136 98-99 18.068473580626794 23.910387569219147 28.87408890795283 29.147049942201225 100 17.73139212402285 23.75841124086029 29.34841329088872 29.161783344228137 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6793.0 1 4404.0 2 3289.0 3 4143.5 4 2851.0 5 2049.5 6 2705.5 7 4407.0 8 5701.0 9 5546.0 10 4522.0 11 3489.0 12 3161.5 13 3206.0 14 3356.5 15 3409.0 16 3244.0 17 3162.0 18 3265.0 19 3459.5 20 3666.5 21 3886.5 22 4126.0 23 4381.5 24 4732.5 25 5366.0 26 6417.0 27 7931.5 28 8757.0 29 9584.5 30 11560.0 31 13821.5 32 16998.0 33 21775.0 34 27318.0 35 32896.0 36 40162.0 37 48182.5 38 53287.0 39 55315.0 40 55957.5 41 56424.5 42 57509.5 43 61037.0 44 65355.5 45 69324.5 46 71151.0 47 69578.5 48 67593.5 49 65373.5 50 61888.5 51 56547.5 52 50847.0 53 45617.5 54 40699.0 55 36162.0 56 31948.5 57 27278.5 58 22887.0 59 19451.0 60 15806.5 61 12457.0 62 9691.5 63 7191.0 64 5239.5 65 3806.0 66 2784.5 67 2192.5 68 1671.0 69 1239.5 70 957.0 71 741.5 72 643.0 73 548.0 74 403.0 75 280.0 76 201.5 77 128.0 78 75.5 79 54.0 80 32.5 81 15.5 82 9.5 83 10.5 84 8.0 85 2.5 86 0.5 87 1.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02919269628382776 2 0.001933291144624355 3 1.933291144624355E-4 4 0.024166139307804438 5 7.088734196955969E-4 6 0.0 7 6.444303815414517E-5 8 0.004124354441865291 9 0.00960201268496763 10-11 9.022025341580323E-4 12-13 1.933291144624355E-4 14-15 9.666455723121775E-5 16-17 5.155443052331614E-4 18-19 0.0 20-21 3.222151907707259E-5 22-23 2.577721526165807E-4 24-25 0.0 26-27 1.2888607630829035E-4 28-29 0.0012566392440058307 30-31 0.003061044312321896 32-33 0.010246443066509082 34-35 0.018978474736395752 36-37 0.02242617727764252 38-39 0.022973943101952755 40-41 0.005091000014177468 42-43 0.005091000014177468 44-45 0.009408683570505194 46-47 0.012179734211133436 48-49 1.2131079717327056 50-51 0.018752924102856245 52-53 0.019075139293626968 54-55 0.009892006356661284 56-57 0.008570924074501308 58-59 0.009698677242198849 60-61 0.006315417739106226 62-63 0.008248708883730582 64-65 0.019365132965320626 66-67 0.027968278558899003 68-69 0.02880603805490289 70-71 0.025326113994579055 72-73 0.023263936773646406 74-75 0.00898980382250325 76-77 0.014145246874834864 78-79 0.008119822807422291 80-81 0.0029966012741677503 82-83 0.014370797508374373 84-85 0.023489487407185913 86-87 0.02307060765918397 88-89 0.030320449451525303 90-91 0.020138449423170366 92-93 0.01308193674529147 94-95 0.007314284830495476 96-97 0.004156575960942363 98-99 0.016400753210229945 100 0.0360881013663213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1551758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.396061118189984 #Duplication Level Percentage of deduplicated Percentage of total 1 82.38863297301714 39.8728531680626 2 10.134240946898807 9.809146885051563 3 2.7176304827656166 3.945678328217428 4 1.2100543539791893 2.3424745788603496 5 0.6883346238123211 1.6656342261893702 6 0.456690786364625 1.326122112540998 7 0.3031798214800843 1.0270896419106463 8 0.22757210410916384 0.8810874767409749 9 0.18057346610744143 0.786514005185323 >10 1.3230616073767645 13.081466942100045 >50 0.21462173577345792 7.3395668888746295 >100 0.14967781252155624 12.98125832528972 >500 0.004010500055752011 1.318511303915343 >1k 0.0010026250139380027 1.0403352951559575 >5k 7.161607242414305E-4 2.582260821904966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9761 0.629028495422611 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9353 0.6027357358557197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6857 0.4418859126229734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6232 0.4016090137766327 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5049 0.325372899640279 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4437 0.2859337602899421 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2817 0.18153603848022695 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2290 0.14757455737299244 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2002 0.12901496238459864 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 5 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 6 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 7 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 8 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 9 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 10-11 0.0 0.0 0.0 6.444303815414517E-5 6.444303815414517E-5 12-13 0.0 0.0 0.0 1.2888607630829035E-4 6.444303815414517E-5 14-15 0.0 0.0 0.0 2.255506335395081E-4 9.666455723121776E-5 16-17 0.0 0.0 0.0 4.1887974800194363E-4 1.2888607630829035E-4 18-19 0.0 0.0 0.0 6.444303815414517E-4 1.9332911446243552E-4 20-21 0.0 0.0 0.0 0.0015788544347765567 1.9332911446243552E-4 22-23 0.0 0.0 0.0 0.005380993685871122 1.9332911446243552E-4 24-25 0.0 0.0 0.0 0.0179796076450065 1.9332911446243552E-4 26-27 0.0 0.0 0.0 0.03837582922079345 1.9332911446243552E-4 28-29 0.0 0.0 0.0 0.07929715844867563 1.9332911446243552E-4 30-31 0.0 0.0 0.0 0.21881633605239992 1.9332911446243552E-4 32-33 0.0 0.0 0.0 0.5895893560722741 1.9332911446243552E-4 34-35 0.0 0.0 0.0 1.1627135158961643 1.9332911446243552E-4 36-37 0.0 0.0 0.0 1.9580694927946238 1.9332911446243552E-4 38-39 0.0 0.0 0.0 3.0066866096388742 1.9332911446243552E-4 40-41 0.0 0.0 0.0 4.109886979799685 1.9332911446243552E-4 42-43 0.0 0.0 0.0 5.203292008161066 1.9332911446243552E-4 44-45 0.0 0.0 0.0 6.466439999020466 2.255506335395081E-4 46-47 0.0 0.0 0.0 7.804954123001138 2.577721526165807E-4 48-49 0.0 0.0 0.0 9.014421063078135 2.577721526165807E-4 50-51 0.0 0.0 0.0 10.20529618664766 2.577721526165807E-4 52-53 0.0 0.0 0.0 11.35144784173821 2.577721526165807E-4 54-55 0.0 0.0 0.0 12.592266319877197 2.577721526165807E-4 56-57 0.0 0.0 0.0 13.952078867967813 2.577721526165807E-4 58-59 0.0 0.0 0.0 15.312600289478127 2.577721526165807E-4 60-61 0.0 0.0 0.0 16.64837558433725 3.2221519077072587E-4 62-63 0.0 0.0 0.0 17.950930493027908 3.8665822892487104E-4 64-65 0.0 0.0 0.0 19.358849769100594 4.1887974800194363E-4 66-67 0.0 0.0 0.0 20.850512773254593 4.5110126707901616E-4 68-69 0.0 0.0 0.0 22.376588359782904 4.5110126707901616E-4 70-71 0.0 0.0 0.0 23.978932281966646 4.5110126707901616E-4 72-73 0.0 0.0 0.0 25.52414745082674 6.444303815414517E-4 74-75 0.0 0.0 0.0 27.095365385582028 6.444303815414517E-4 76-77 0.0 0.0 0.0 28.692102763446364 6.444303815414517E-4 78-79 0.0 0.0 0.0 30.33279029333182 6.444303815414517E-4 80-81 0.0 0.0 0.0 31.976828861201298 6.766519006185243E-4 82-83 0.0 0.0 0.0 33.57907611882781 7.088734196955969E-4 84-85 0.0 0.0 0.0 35.14774855357601 7.088734196955969E-4 86-87 0.0 0.0 0.0 36.717355412377444 7.410949387726695E-4 88 0.0 0.0 0.0 37.88251776372347 7.733164578497421E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4510 0.0 37.157864 1 GTATCAA 7370 0.0 34.20313 1 TATCACG 55 0.0027412344 34.12073 2 TACCTGG 785 0.0 30.480461 2 ATCAACG 9360 0.0 26.870762 3 CAACGCA 9490 0.0 26.397823 5 TCAACGC 9565 0.0 26.343918 4 CATATAG 290 0.0 25.888865 3 TATCAAC 9785 0.0 25.84336 2 AACGCAG 9830 0.0 25.43623 6 ACCTGGG 990 0.0 24.646723 3 GTACGCA 135 1.4719446E-5 24.340155 1 ACCGTCC 135 1.4793144E-5 24.324465 8 GTACACG 140 1.9505105E-5 23.470863 1 ACGCAGA 10945 0.0 22.844965 7 GACCGAC 125 2.4550932E-4 22.517504 7 CGCAGAG 11125 0.0 22.433168 8 GTGGTAT 3025 0.0 22.035456 1 GTATAGT 505 0.0 21.3794 1 GCAGAGT 11670 0.0 21.14433 9 >>END_MODULE