FastQCFastQC Report
Mon 6 Jun 2016
ERR1211363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9121319
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267990.2938061918457188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202970.22252264173635417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125840.1379625030108036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131100.049.1911281
GTATCAA241350.041.6884271
TACCTGG73400.033.9635242
CAACGCA294150.033.0825085
ATCAACG296950.032.8032953
TATCAAC309100.031.8799742
TCAACGC308600.031.6564674
AACGCAG315050.031.349526
GTACATG358800.028.2387371
ACGCAGA359200.027.288347
TACATGG367800.027.02222
GTACCTG97650.026.590031
CGCAGAG370550.026.3369168
ACATGGG373100.025.7554743
ACCTGGG95700.025.6797773
GCAGAGT406700.023.3738549
CATGGGG253450.023.0082384
GTACTAG30850.022.5662061
TAACGCC14000.021.1454224
TCTAACG14500.021.0852282
Produced by FastQC (version 0.11.5)