FastQCFastQC Report
Mon 6 Jun 2016
ERR1211363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9121319
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236280.2590414829258795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188290.20642847816198512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123950.13589043426723701No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133750.046.2847331
GTATCAA237350.040.6753961
TACCTGG72900.034.0470242
CAACGCA295550.031.7281485
ATCAACG297850.031.475853
TCAACGC309800.030.4133474
TATCAAC313550.030.3916382
AACGCAG318550.029.8054566
GTACCTG97800.026.2983471
GTACATG350400.026.1994111
ACCTGGG94250.025.9877513
ACGCAGA366050.025.7199347
TACATGG363550.025.0502682
CGCAGAG377250.024.8444358
ACATGGG359900.024.5237273
GTACTAG28900.023.5481571
CATGGGG238550.022.2663444
GCAGAGT417850.022.1040339
CTAACGC15250.021.8458063
TCTAACG15950.021.1798482
Produced by FastQC (version 0.11.5)